Coat color was an economic trait in sheep, and identifying the causal variations affecting its diverse phenotypes had long been of interest. We performed whole-genome sequencing (WGS) to investigate the genetic composition and conducted a genome-wide association study (GWAS) for coat color using our lab data for 115 sheep (80 newly sequenced) from four Liangshan populations. Additionally, we retrieved public data for 104 sheep (from six other breeds) and 44 wild sheep. Our study revealed that the Liangshan sheep population exhibited moderate genetic diversity and a closer genetic relationship with Yunnan sheep. Using WGS data, we performed a GWAS to identify genetic loci and causal variants influencing the black coat pigmentation in 115 Liangshan sheep (i.e., 85 white coat LSS vs. 30 black coat LSB). GWAS identified a 1.526 Mb region (including the MC1R gene) on CHI14, spanning from 12,982,495 bp to 14,508,891 bp, as significantly associated with black coat (− log10P > 8.67). MC1R was highly expressed in the LSB group (mean = 15.394), significantly higher than in the LSS group (mean = 2.371; P = 0.032). A contingency table analysis revealed a highly significant difference in genotype frequencies at the MC1R mutation site c.361G > A between the LSB and LSS populations (Fisher’s exact test, P = 8.268 × 10–25). Molecular docking results further demonstrated that the c.361G > A mutation in MC1R of the LSB population disrupted a hydrogen bond between MC1R residue Asn121 and ASIP residue Phe119, reducing their molecular interaction. Haplotype analysis showed that 96.67

Understanding the genetic makeup of local sheep breeds is essential for their scientific conservation and sustainable utilization. The Liangshan semi-fine-wool sheep (LSS), a Chinese semi-fine-wool breed renowned for its soft wool, was analyzed using whole-genome sequencing data including 35 LSS, 84 sheep from other domestic breeds, and 20 Asiatic mouflons. We investigated the genetic composition of LSS by conducting analyses of the population structure, runs of homozygosity, genomic inbreeding coefficients, and selection signature. Our findings indicated that LSS shares greater genetic similarity with Border Leicester and Romney sheep than with Tibetan (TIB), Yunnan (YNS), and Chinese Merino sheep. Genomic analysis indicated low to moderate inbreeding coefficients, ranging from 0.014 to 0.154. In identifying selection signals across the LSS genome, we pinpointed 195 candidate regions housing 74 annotated genes (e.g., IRF2BP2, BVES, and ALOX5). We also found the overlaps between the candidate regions and several known quantitative trait loci related to wool traits, such as the wool staple length and wool fiber diameter. A selective sweep region, marked by the highest value of cross-population extended haplotype homozygosity, encompassed IRF2BP2—an influential candidate gene affecting fleece fiber traits. Furthermore, notable differences in genotype frequency at a mutation site (c.1051 + 46T > C, Chr25: 6,784,190 bp) within IRF2BP2 were observed between LSS and TIB and YNS sheep (Fisher’s exact test, p < 2.2 × 10−16). Taken together, these findings offer insights crucial for the conservation and breeding enhancement of LSS.

MicroRNAs (miRNAs) are important regulators of gene expression and are involved in bacterial pathogenesis and host-pathogen interactions. In this study, we investigated the function of miRNAs in the regulation of host responses to Pasteurella multocida infection. Using next-generation sequencing, we analyzed miRNA expression pattern and identified differentially expressed miRNAs in Pasteurella multocida-infected goat lungs. In addition, we investigated the function of differentially expressed miRNAs andtheir targeted signaling pathways in bacterial infection processes. The results showed that Pasteurella multocida infection led to 69 significantly differentially expressed miRNAs, including 28 known annotated miRNAs with miR-497-3p showing the most significant difference. Gene target prediction and functional enrichment analyses showed that the target genes were mainly involved in cell proliferation, regulation of the cellular metabolic process, positive regulation of cellular process, cellular senescence, PI3K-Akt signaling pathway, FoxO signaling pathway and infection-related pathways. In conclusion, these data provide a new perspective on the roles of miRNAs in Pasteurella multocida infection.

Urea, as a feed additive, causes poisoning in excessive dosage. In this study, we explored the mechanism of lung damage in urea poisoning on Jianzhou Da'er goat by adding different levels of urea. We used pathological histological analysis and Real-time fluorescence quantitative PCR to investigate the extent of lung damage. 9 Jianzhou Da'er goats of similar age and body condition were selected and equally randomized into three groups: 0 % urea group with 0 urea diet, 5 % urea group with 5 % urea diet and 10 % urea group with 10 % urea diet. Equal feeding was used 3 times daily (8:00 a.m., 2:00 p.m., 8:00 p.m.) in 7-d pre-feeding stage and 21-d formal experiment. After the experiment, we found the lungs of 5 % urea group and 10 % urea group both had collagen fibrosis proliferation and hemosiderin production. The nuclei of alveolar epithelial cells in the 5 % urea group were constricted and the mitochondria swelled; the alveolar epithelial cells in the 10 % urea group were necrotic and disintegrated. The expressions of RIP3 (Receptor interacting protein kinase 3) and TNF-alpha (tumor necrosis factor-alpha) mRNA in the lung of the 5 % urea group and 10 % urea group were increased significantly (P < 0.01) while the mRNA expressions of cIAP2 (cellular inhibitor of apoptosis 2) and TRADD (TNFR1-associated death domain) in the 10 % urea group were significantly decreased (P < 0.01); the mRNA expression of NEMO (NF-kappa B essential modulator) and Caspase-8 (cysteine aspartate protease-8) in lung of the 5 % urea group and 10 % urea group were significantly decreased (P < 0.01) and the mRNA expression of HMBG-1 (High mobility group box-1) and HSP70(Heat Shock Protein 70) were significantly up-regulated (P < 0.01). The expression of RIP3 protein in 5 % urea group and 10 % urea group were increased significantly (P < 0.01). In summary, evidence of lung damage from urea feeding includes disruption of alveolar structure, lysis of organelles, and a significant rise in genes associated with necroptosis. We expect to provide scientific value for the study of necroptosis and lung injury caused by urea toxicity.

The main aim of the current work was to explore the differential metabolites and differentially expressed genes of longissimus dorsi muscle (LDM) between castrated and uncastrated fattening male South Sichuan black goats ( Capra hircus ). Then, the key genes regulating important differential metabolites (DMs) in castrated male goats were observed by integrated metabolomics and transcriptomics analyses. In addition, we evaluated the effects of castration on blood constituents, dressing percentage, and water holding capacity of LDM in male black goats. The results showed that the concentrations of alkaline phosphatase (ALP), total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) were significantly increased and testosterone was significantly decreased in castrated male goats compared with the uncastrated male goats, while dressing percentage of black goats and water holding capacity of longissimus dorsi muscle were not significant differences. Through metabolomics and transcriptomics analyses, 23 important KEGG pathways, 13 important DMs, 32 important differentially expressed genes (DEGs), and 13 key genes related to the “Metabolism” and “Organismal systems” pathways were screened. Lipid accumulation may be elevated in the blood of fattening South Sichuan black goats after castration. Castration might play a positive role in energy provision, intercellular signaling, muscle function, softening of meat, disease reduction, and anti-oxidation of LDM. P4HA2, AKR1B1, GPT2, L2HGDH, ENSCHIG00000021660, ENSCHIG00000023861, DGAT2, ULK1, SLC38A3, PLA2G4A, SLC6A1, ENSCHIG00000026624, and ND2 might be the key genes regulating important DMs in the KEGG pathways related to “Metabolism” and “Organismal systems” of castrated male goats compared with the uncastrated male goats.

为了探究从川南黑山羊体内分离获得的1株细菌LZS-01的致病性和生物学特性,本试验采用革兰染色、细菌形态观察、生化鉴定、16S rDNA基因序列分析、小鼠致病性试验和药敏试验对其进行研究.结果显示:菌株LZS-01为短杆状革兰阴性菌,16S rDNA基因扩增后得到的DNA片段长度为1 446 bp,与GenBank中波氏希瓦氏菌(Shewanella baltica)的同源性为100％,在系统进化树中与波氏希瓦氏菌(NCTC10737)聚为一支,综上结果推测该菌株为波氏希瓦氏菌;该菌株对小鼠致病性较强,可导致其肺泡壁增厚、肺泡腔狭窄,纤维样物质充满肺泡,肾小管上皮细胞水肿严重、管腔狭窄等;该菌株对环丙沙星、四环素等抗菌药物敏感,对氟苯尼考、青霉素和氧氟沙星等抗菌药物耐药.结果表明,波氏希瓦氏菌对动物具有一定致病性和耐药性.本试验结果为波氏希瓦氏菌感染性疾病的研究和临床防治积累了科学资料.

The aim of the present study was to evaluate the effects of Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09 on the expressions of spleen genes in weanling Jintang black goats. Bacillus amyloliquefaciens fsznc-06 (BA-treated group) and Bacillus pumilus fsznc-09 (BP-treated group) were directly fed to goats, and the spleens were harvested for transcriptome analysis. The KEGG pathway analysis showed that the differentially expressed genes (DEGs) in BA-treated vs CON group were mainly involved in digestive system and immune system, while those in BP-treated vs CON group were mainly involved in immune system, and those in BA-treated vs BP-treated group were mainly involved in digestive system. In conclusion, Bacillus amyloliquefaciens fsznc-06 might promote the expressions of genes related to immune system and digestive system, reduce the expressions of disease genes related to digestive system and might promote mutual accommodation of some immune genes in weanling black goat. Bacillus pumilus fsznc-09 might promote the expressions of genes related to immune system and mutual accommodation of some immune genes in weanling black goat. Bacillus amyloliquefaciens fsznc-06 has advantages over Bacillus pumilus fsznc-09 in promoting the expressions of genes related to digestive system and mutual accommodation of some immune genes.

[Objectives]The purpose of this study was to explore the pathological and molecular mechanisms of kidney injury in goats caused by urea. [Methods]Nine healthy and disease-free Jianzhou Da’er goats were selected and randomly divided into 3 groups, including control group（C group）,5% urea group（U5 group）and 10% urea group（U10 group）. The feeding method was crude concentrate mixed feeding, and the urea was used to replace part of soybean meal concentrate feeding. Urea was not added to control group. The trial period was 21 days. At the end of the trial, blood samples were collected, serum blood ammonia levels were measured, the indicators of oxidative damage and iron ion content in kidney tissue were determined by biochemical and iron kit. The pathological damage of kidney tissue were observed by HE and Masson stain. The ultrastructure of renal tubular epithelial cells was observed by transmission electron microscope. The mRNA relative transcription level of ferroptosis-related genes in kidney tissue were detected by RT-qPCR. The expression level of recombinant glutathione peroxidase 4（GPX4）protein was detected by Western blot. [Results]Compared with the control group, the content of malonic dialdehyde（MDA）in the 5% urea group and the 10% urea group significantly increased（P＜0.01）,but the content of L-glutathione（GSH）,the activities of SOD（superoxide dismutase）and GSH-Px（glutathione peroxidase）significantly decreased（P＜0.01）. Meanwhile, the content of iron ions significantly increased（P＜0.01）. The epithelial cells of renal tubules were swollen, denaturated and necrotic. Collagen fibers were hyperplasia, and the mitochondria of renal tubular epithelial cells atrophied, cristae reduced, and membrane density was increased. The mRNA relative transcription level of FTH1（ferritin, heavy polypeptide 1）,VDAC3（voltage dependent anion channel protein 3）,p53（Tumor protein 53）and NCOA4（nuclear receptor coactivator 4）genes significantly increased（P＜0.01）. The mRNA levels and protein expression level of GPX4 significantly decreased（P＜0.05）. [Conclisions]The expression of NRF2 was inhibited when urea was added at high dose, initiating the mechanism of cellular iron death, causing oxidative stress in kidney and iron ion metabolism imbalance.

为了监测凉山半细毛羊种群生产性能的变化情况,了解不同年龄段羊只的羊毛品质指标和生长发育性能,本试验对108只不同性别和年龄的凉山半细毛羊的体高、体尺和羊毛品质进行了检测,结果显示:(1)育成公羊的体重和体高、体长和胸围分别显著和极显著高于育成母羊,除了管围以外,成年公羊的体重和体尺均极显著高于成年母羊(P＜0.01);(2)同一年龄同一部位的羊毛纤维直径母羊均大于公羊,育成母羊肩部的纤维直径极显著高于育成公羊(P＜0.01),成年母羊侧部的羊毛纤维直径显著高于成年公羊(P＜0.05);(3)育成羊的毛丛自然长度、卷曲度和伸直率之间无显著差异(P＞0.05),成年母羊各部位的毛丛自然长度均显著高于成年公羊(P＜0.05),而成年公羊肩部的卷曲度显著高于成年母羊(P＜0.05);(4)育成羊的羊毛白度范围在65.30～67.19,光泽度为66.03～68.14,育成期公母羊的羊毛白度(Wht)和光泽度(L)无显著差异(P＞0.05),育成公羊肩部和股部的羊毛黄度(YI)显著高于育成母羊(P＜0.05),两者分别相差2.56和2.86,成年公羊各个部位的羊毛白度和光泽度以及侧部和股部的羊毛黄度极显著高于成年母羊(P＜0.01).本研究表明,凉山半细毛羊公羊的体重和体尺优于母羊,该品种羊的羊毛品质较好,但其卷曲度和伸直率仍有改善的空间.

近年来四川省肉羊生产有了长足的发展,当年羔羊育肥出栏占的比例越来越大.但与国内外发达地区相比,四川省肉羊生产存在肉羊胴体重低、屠宰率低、饲养成本高、收益少等关键技术问题.项目系统开展肥羔生产的优良品种选择及杂交组合筛选、饲草饲料供给技术及相关饲料配方研制、肥羔羊肉品质、场舍建设设计、主要疫病综合防控技术等研究,创新集成了四川山羊肥羔生产技术,并进行了推广应用.

Aspergillus nidulans (A. nidulans) is an ascomycetous fungus that can cause disseminated infection in humans or animals. The present study aimed to explore the pathogenesis and host spleen immune response after infection with A. nidulans. Thirty KM mice were divided into control group (C) and treated group (T). Serum was collected for the detection of inflammatory markers. Spleens were collected for histopathological examination, fungal culture and transcriptomic analysis. Compared with the control group, the concentrations of serum total protein (TP), globulin (GLO) and C-reactive protein (CRP) were significantly increased (P < 0.01), but the concentration of albumin (ALB) and the ratio of albumin/globulin (A/G) were significantly decreased in the treated group (P < 0.01). In addition, the splenic red pulp was hyperemic, and the white pulp was infiltrated by inflammatory cells in treated group. The chromatin was aggregated, and the mitochondria were swollen in the spleen lymphocytes of treated group. Transcriptome sequencing results showed that 47.2 million and 44.9 million clean reads were obtained in the control group and treated group, respectively. 946 differentially expressed genes (DEGs) were obtained, including 372 up-regulated genes and 574 down-regulated genes. GO analysis showed that these DEGs were mainly involved in the immune responses, antigen binding, immunoglobulin receptor binding, bacterial defence responses, endopeptidase activity and so on. Moreover, KEGG analysis showed that these DEGs were mainly enriched in the following pathways: complement and coagulation cascades, retinol metabolism, cytochrome p450, IL-17 signalling pathway and so on. Additionally, 9 DEGs was validated by qRT-PCR approach. In summary, this study revealed the immune response mechanism of spleen in A. nidulan-infected mice. It will benefit for a better understanding of the pathogenic mechanisms of A. nidulans.

The present study aimed to explore the toxic effects of excessive dietary Mn in livers and cerebrums of Jianzhou Da'er goat (Capra hircus). Three-month old goats were assigned into three groups: control group, fed on basal diet; Mn I group, fed on the basal diet mixed with MnCl2 (2.5 g/kg); Mn II group, fed on the basal diet mixed with MnCl2 (5 g/kg). Compared with the control group, the activities of serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and the concentrations of interferon-γ (IFN-γ) in Mn I and Mn II groups were significantly increased, but the concentrations of IgG in Mn I and Mn II groups were significantly decreased (p < 0.05). The activities of superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and the concentrations of glutathione (GSH) in Mn I and Mn II groups were significantly decreased, whereas the concentrations of malondialdehyde (MDA) in Mn I and Mn II groups were significantly increased in livers and cerebrums (p < 0.05). Moreover, the hepatocytes necrosed, inflammatory cells infiltrated, chromatin concentrated, mitochondrial cristae reduced in Mn I and Mn II groups. The nerve cells necrosed, blood vessels congested, inflammatory cells infiltrated, mitochondrial electron density and mitochondrial cristae decreased, and vacuolization increased in Mn I and Mn II groups. Furthermore, the mRNA expressions of tumor necrosis factor alpha (TNF-α), tumor necrosis factor receptor type 1 (TNFR1), fas-associated protein via a death domain (FADD), Bcl2-associated X (Bax), cysteinyl aspartate specific proteinase 3, 8, 9 (Caspase-3, 8, 9) in Mn I and Mn II groups were significantly increased (p < 0.05), but the mRNA expressions of B-cell lymphoma-2 (Bcl-2) in Mn I and Mn II groups were significantly decreased (p < 0.05) in livers. The mRNA expressions of Bcl-2, Bax, Caspase-3, 9, 7, 12 in Mn I and Mn II groups were significantly increased (p < 0.05), however, the ratio of Bcl-2/Bax in Mn I and Mn II groups was significantly decreased (p < 0.05) in cerebrums. In summary, our results provided new insights for better understanding the mechanisms of Mn toxicity in Capra hircus.

Inflammatory chemokine CCL5 can mediate the occurrence of inflammatory reactions and participate in various disease processes. (Ch)CCL5 gene of Jintang black goat (Capra hircus, C. hircus) was cloned. The CDS (coding sequences) was 276 bp in length and encoded 91 amino acids. The 26.5 kDa recombinant protein was expressed by Escherichia coli system and purified by Ni-Agarose. The viabilities of primary goat lung fibroblasts could be enhanced after treating with ChCCL5 protein (12.5, 25, 50 μg/mL) (P < 0.05). The expression levels of interleukin-1beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor (TNF-α), C-C motif chemokine ligand 2 (CCL2) and heat-shock proteins (Hsp70) genes were upregulated after treating with ChCCL5 protein (12.5, 25, 50 μg/mL). Besides, the viabilities and phagocytic abilities of primary mouse peritoneal macrophages could be enhanced after treating with ChCCL5 protein (12.5, 25, 50 μg/mL) (P < 0.05). The expression levels of IL-1β, IL-6, toll-like receptor 4 (TLR4), inducible nitric oxide synthase (iNOs) and TNF-α genes were upregulated after treating with ChCCL5 protein (12.5, 25, 50 μg/mL) (P < 0.05). These results indicated that goat CCL5 might play a role in the inflammatory response by regulating the inflammatory cytokines produced by lung fibroblasts and macrophages.

肥羔型黑山羊是四川省畜牧科学研究院以川南黑山羊(自贡型)为主要育种材料培育的专门化从事羔羊肉生产的肉用山羊新品种.肌纤维数目与大小是决定肌肉生长的主要因素,而肌纤维类型及组成是肉品质的生化和分子生物学基础,分析研究肌纤维不同阶段的生长发育状况,将为后续发育生物学的研究,从而进行营养调控提供理论依据.本研究通过对不同月龄阶段组(0～6月龄)的肥羔型黑山羊体质量进行记录,采用组织切片和苏木素-伊红染色对肌纤维直径、面积大小、数量进行测定,绘制出肥羔型黑山羊体质量和肌纤维生长发育曲线图.结果表明,肥羔型黑山羊在整个养殖周期都保持2.95～4.35 kg/30 d的较高绝对生长强度,体质量持续显著提高,而相对生长系数由30日龄时的93.05％降到180日龄时的13.83％.本研究发现,肥羔型黑山羊的肌纤维直径和面积呈现明显的"三段式"生长发育特点,并且在3个阶段表现出不同性别优势的趋势.对肥羔型黑山羊肌纤维生长发育规律的研究有助于进一步优化生产关键配套技术和促进四川省肉羊产业的多元化全面发展.

为分析山羊KLF16对肌内前体脂肪细胞分化的调控作用,利用RT-PCR克隆得到山羊KLF16基因序列,利用生物信息学分析山羊KLF16基因序列特征,通过过表达、油红O染色和qPCR等方法从形态学及分子水平分析过表达山羊KLF16后肌内脂肪细胞脂滴积聚及分化标志基因表达水平的变化.结果表明:1)克隆得到包含CDS区的山羊KLF16基因序列986 bp,编码251个氨基酸,具有典型锌指结构;2)KLF16在山羊各组织广泛表达,其中腹部皮下脂肪组织表达量极显著高于其他组织(P＜0.01);3)过表达山羊KLF16与对照组相比脂肪细胞脂滴积聚减少,分化标志基因PPARγ和PPARα极显著下调(P＜0.01),C/EBPβ显著下调(P＜0.05);4)过表达山羊KLF16后KLFs部分成员发生显著的上调或下调,相关性分析结合转录因子结合位点预测结果推测KLF16可能通过拮抗KLF8来发挥其对分化的调控作用.综上,山羊KLF16可能通过拮抗KLF8抑制分化标志基因PPARγ、PPARα 和 C/EBPβ的表达,从而抑制肌内前体脂肪细胞分化,为揭示山羊KLF16调控脂肪细胞分化提供重要的数据支持.

从孕羊流产死胎中分离鉴定菌株AEV001,采用生理生化、16SrDNA基因扩增、动物回归、耐药表型和耐药基因型检测等方法,探究该菌株的生物学特性.结果显示:菌株AEV001为革兰阳性菌,蔗糖、硝酸盐还原和乳糖反应为阳性,木糖苷、蕈糖和蜜二糖等反应为阴性.PCR扩增得到16S rDNA基因片段为1 466 bp,系统进化树中菌株AEV001与绿色气球菌聚为一类,从而判断菌株AEV001为绿色气球菌.在该菌株中检出lytA和Fbps2个毒力基因.试验组小鼠肺脏出现肺泡壁增厚,炎性细胞浸润,肾小管上皮细胞肿胀变性.菌株AEV001检出Sul1、Sul2、aac(6')-Ⅰb、ant(3")-Ia和TEM耐药基因,并对诺氟沙星、阿莫西林和氨苄西林等药物敏感,对阿米卡星耐药.研究结果为绿色气球菌感染性疾病的研究积累了科学资料.

旨在明确miR-301b对山羊肌内脂肪细胞分化的调控作用，探讨其发挥调控作用的可能机制。利用化学合成的miR-301b mimics和miR-301b siRNA，通过脂质体转染法在山羊肌内脂肪细胞过表达和干扰miR-301b，并通过油红O染色、qPCR和生物信息学分析等方法，从细胞形态学和mRNA水平明确miR-301b对山羊肌内脂肪细胞分化的调控作用，并初步探究其可能的作用机制。结果表明，过表达miR-301b效率约29 290倍，细胞形态学结果显示，过表达miR-301b后山羊肌内脂肪细胞脂滴积聚减少，甘油三酯合成降低。qPCR结果显示，过表达miR-301b后成脂标志基因CEBPα、PPARγ、SREBP1、LPL表达水平极显著下调（P＜0.01）。miR-301b干扰效率约60%，干扰miR-301b表达后，细胞形态学结果显示，山羊肌内脂肪细胞脂滴积聚增加，甘油三酯合成升高。qPCR结果显示，干扰miR-301b后成脂标志基因AP2、CEBPα、CEBPβ、PPARγ、SREBP1、LPL表达水平极显著上调（P＜0.01）。通过对miR-301b生物信息学分析，推测KLF3为miR-301b的靶标基因，qPCR验证发现过表达miR-301b后显著抑制KLF3的mRNA表达水平（P＜0.05），干扰miR-301b后显著促进KLF3的表达水平（P＜0.05）。过表达miR-301b抑制山羊肌内前体脂肪细胞分化，同时KLF3表达水平下调；干扰miR-301b促进山羊肌内前体脂肪细胞分化，KLF3表达水平上调，推测miR-301b可能通过KLF3抑制山羊肌内脂肪细胞分化。

为确定四川成都某羊场黑山羊发病的病原并探讨其病理变化特点,本试验观察了患病羊的临床症状及剖检变化,制备了其唇、舌、心脏、肝脏、脾脏、肺脏的病理组织切片,观察其病理变化;并对患病羊的口唇病料样品进行羊传染性脓疱病毒(CEV)F1L基因的PCR检测.剖检结果显示,患病金堂黑山羊口、唇出现大小不等的丘疹、溃疡和脓疱;心包积液、出血;肾脏、脾脏肿大、淤血.病理组织学检查结果显示,病变唇、舌部表皮棘细胞大量增生,棘细胞空泡变性、网状变性、气球样变性,胞浆内可见嗜酸性病毒包涵体;心肌细胞排列紊乱,心肌纤维肿胀,成纤维细胞增多;肝细胞水肿,空泡变性;脾淋巴鞘增厚,血窦扩张、充血;肺泡扩张、破裂,肺泡壁变薄.PCR检测结果显示,患病羊口唇病料样品扩增出大小为230 bp的F1L基因片段;测序后BLAST比对显示,该基因序列与GenBank中登录的羊传染性脓疱病毒F1L基因序列同源性达99％.上述结果表明,该羊场此次发病由CEV感染引起,且CEV主要侵害金堂黑山羊唇、舌部的上皮组织,并且可造成心肌细胞肿胀、肝细胞变性、脾窦充血、肺泡扩张等病理损伤.本试验结果为CEV的诊断与治疗提供了科学依据.

为制备羊源解淀粉芽孢杆菌fsznc-06菌.剂,采用响应面法,分析优化冻干保护剂配方,从离心条件、保护剂筛选与复配、稳定性检测等方面探究对菌体存活率的影响.结果 表明,最佳离心条件为6000 r/min离心10 min,优化后的冻干保护剂配方为丙三醇(3.102 g/100 mL)、蔗糖(2.926 g/100 mL)和葡萄糖(7.096 g/100 mL),在该条件下解淀粉芽孢杆菌存活率达到96.47％,与理论预测值接近.冻干菌剂在4℃条件下贮存稳定性更高,保藏180天后的存活率约为66.30％,说明响应面法可用于优化解淀粉芽孢杆菌冻干保护剂.

为了探究肥羔型黑山羊繁殖及生长发育规律,给新品种选育提供参考,本试验测定了 177只母羊的繁殖性状和初生、1月龄、2月龄、4月龄、6月龄羊的体重体尺数据,并进行统计分析.研究表明,肥羔型黑山羊多胎性好、早期生长发育较快,具有进一步选育的价值.