This study aimed to identify proteins associated with high-fat diet patients and investigate their relationship with this dietary pattern. Five hyperlipidemia female patients and five normal individuals were included as the experiment and control groups, respectively. Blood samples were collected from both groups, and bioinformatics tools were employed for gene ontology annotation, KEGG pathway annotation, GO enrichment analysis, pathway enrichment analysis, and protein clustering to pinpoint genes, proteins, and pathways relevant to high-fat diet patients. Mass spectrometry analysis was subsequently used to confirm these proteins. The results indicated that bioinformatics analysis identified several proteins (P09871, P01019, P48740, P02654, P02649) potentially involved in the high-fat diet process by regulating downstream pathways. Label-free analysis revealed 3915 peptides in both groups, with 16 protein expression levels up-regulated in the experiment group, 13 of which showed significant differences. In contrast, 12 protein expression levels were down-regulated in the experiment group, with two showing significant differences. Notably, the proteins highlighted by bioinformatics analysis aligned with those identified through mass spectrometry. In conclusion, label-free analysis combined with bioinformatics can effectively identify proteins linked to high-fat diet patients. This research provides a fresh perspective on addressing high-fat diet-related issues using this approach.

目的 探讨失眠的认知行为治疗(CBT-I)对围产期失眠、焦虑抑郁情绪及疲劳状况的疗效.方法 收集2019年6月至12月在无锡市妇幼保健院门诊就诊的伴有焦虑抑郁症状的妊娠期失眠患者68例,使用随机数字表法,将其分为CBT-I组34例,控制组34例,共治疗8周.在治疗前、治疗后及12周随访时记录睡眠日记,包括入睡潜伏期(SOL)、觉醒时间(WASO)、入睡后总觉醒次数(NOA)、总睡眠时间(TST)、总卧床时间(TIB),并采用匹兹堡睡眠质量指数(PSQI)、失眠严重程度指数(ISI)、爱丁堡产后抑郁量表(EPDS)、状态-特质焦虑量表(STAI)、多维疲劳量表(MFI-20)进行评估分析.结果 CBT-I治疗后,CBT-I组SOL、WASO、NOA、TIB、ISI、PSQI、EPDS、STAI和MFI-20得分均显著低于控制组(t值分别为-4.82、-11.06、-0.29、-11.35、-2.41、-0.41、-0.94、-10.88、-7.12,均P<0.05),T S T得分显著高于控制组(t=7.82,P<0.05);治疗结束12周随访时,CB T-I组上述各指标得分与控制组比较差异仍均有统计学意义(t值分别为-6.47、-5.35、-0.35、6.94、-11.18、-3.82、-1.47、-1.41、-4.94、-8.06,均P<0.05).结论 CBT-I对孕妇客观睡眠质量和主观睡眠感受及孕妇焦虑抑郁情绪和疲劳症状的改善均有一定的疗效.

目的 分析对绝经后阴道壁病变激光治疗前雌激素预处理的临床价值.方法 采用前瞻性研究方法,选取2016年4月至2018年6月期间106例南京医科大学附属无锡市妇幼保健院收治的择期实施激光治疗的绝经后阴道壁病变患者为研究对象,按照随机数字表法将其分为常规组和研究组,每组各53例.常规组患者在激光治疗后给予阴道雌激素给药处理,研究组患者分别在激光治疗前和治疗后给予阴道雌激素给药处理.比较两组患者的手术治疗效果、术后康复情况及并发症发生率.结果 研究组患者的治疗有效率为92.45％,较常规组(77.63％)高,差异具有统计学意义(χ2=4.711,P<0.05);研究组患者的术后创面愈合时间[(18.69±5.36)d]、阴道流液时间[(6.35±1.20)d]均较常规组[(24.42±3.56)d、(7.43±1.58)d]短,治疗次数[(2.02±0.37)次]较常规组[(2.31±0.54)次]少,差异具有统计学意义(t=6.483、3.963、3.225,P<0.05);研究组患者的术后创面感染、创面出血发生率分别为5.66％、7.55％,较常规组(20.75％、22.64％)低,差异具有统计学意义(χ2=5.267、4.711,P<0.05).结论 对绝经后阴道壁病变激光治疗前实施雌激素预处理,可提升手术治疗效果,提升术后康复速度,降低术后并发症发生率,效果显著.

目的 比较不同类型阴道炎患者宫颈人乳头瘤病毒(HPV)感染情况.方法 回顾性分析2018年1月至2019年12月南京医科大学附属无锡市妇幼保健院收治的1453例同时进行HPV检测及液基薄层细胞(TCT)检查者的临床资料,分析霉菌性阴道炎、滴虫性阴道炎、细菌性阴道炎与正常对照组HPV的感染情况.结果 细菌性阴道炎HPV阳性率为29.31％,较正常对照组12.54％高,差异具有统计学意义(P<0.05);滴虫性阴道炎、霉菌性阴道炎HPV感染率分别为17.65％、11.63％,均与正常对照组相近,差异无统计学意义(P>0.05);细菌性阴道炎各个年龄段发病率均与HPV感染呈正相关(P<0.05).滴虫性阴道炎、霉菌性阴道炎与HPV感染未见相关性(P>0.05).结论 阴道炎会增加宫颈HPV感染风险,其中细菌性阴道炎宫颈HPV感染率更高.

Objective To investigate the serum markers of CA153, CA125,CEA, CA199 in the early diagnosis of breast cancer and clinical application value. Chemiluminescence immunoassay detectionmethod For the determination of 37 cases of benign breast lesions and 52 cases of I, IIbreast cancer patients before treatment the serum CA153 detection,CA125, CEA, CA199 parameters by using the method. The results of determination of CA153, CA199 values were significantly higher than that ofbenign tumor group (P&lt; 0.05), breast cancer in the group of CA153,CA199 positive detection rate and a four positive detection rate compared with the control group with significant difference (P&lt; 0.05).Conclusion The combined four detection sensitivity and accuracy is beter than the single detection of breast cancer, 1 phase II, deficiencies can compensate each other single indicator.

Objective To investigate the value of joint detection used in cervical cancer screening of outpatients. Methods The study objects were the 812 women that in cervical cancer screening in cervical clinic of our hospital. There were three methods of screening,they were TCT,HPV-DNA and visual inspection; the positive women of the three methods of screening than to deteted with colposcopy. The histopathological results were served as the gold standard. Results The susceptibility,specificity,positive predictive value and negative predictive value of the TCT were 75. 0%,97. 2%,35. 3%,99. 5% respectively; The susceptibility,specificity,positive predictive value and negative predictive value of the HPV-DNA were 81. 3%,77. 2%,6. 7%,99. 5% respectively; The susceptibility,specificity,positive predictive value and negative predictive value of the visual inspection were 75. 0%,84. 5%,8. 9%,99. 4% respectively.Conclusions The TCT were better than the other two methods,the TCT can be the first choice of cervical cancer screening. HPV-DNA and visual inspection had the same value.

目的:探讨口服避孕药对青春期原发性痛经的疗效,为治疗原发性痛经寻找新途径.方法:选取青春期中、重度原发性痛经患者90例,分为2组,观察组使用口服避孕药进行治疗,对照组使用安慰剂维生素E治疗,就诊后第2个月经周期的第5天开始服用药物,治疗前及治疗1个月后进行疼痛及经期不适评估,用药3个月后比较疗效,治疗前及治疗3个月检测所有患者静脉血中前列腺素PGE2和PGF2α水平.结果:用药1个月后观察组患者目测类比疼痛评分(VAS)出现了明显的下降(P＜0.05),达到(3.32±1.95)分,而对照组的下降程度则无显著性(P＞0.05),治疗后观察组MDQ显著低于对照组(P=0.011);用药3个月后观察组的疗效显著高于对照组(P＜0.01);而治疗3个月后观察组的前列腺素E2 (PGE2)和前列腺素F22α(PGF2α)水平分别达到(294.7±126.7) ng·mL-1和(317.3±128.4) ng·mL-1,均显著低于对照组(P=0.048;P=0.038).结论:口服避孕药使青春期原发性患者的血液内前列腺素水平明显降低,疼痛感和经期不适反应都得到减轻,疗效具有显著性,值得临床推广使用.

<正>异位妊娠(EP)是孕妇死亡的重要原因之一,延误诊治是导致死亡的潜在因素,因此EP的早期诊断是妇产科关心的问题,探讨阴道B超在EP早期诊断中的应用价值尤为重要。现对本院2007年1～12月临床疑诊EP145例病人进行腹部超声及阴道超声检查,现报道如下。