Background:Lateral epicondylitis (LE) is a prevalent musculoskeletal disorder characterized by pain and functional limitations of the elbow. Acupuncture is increasingly employed as an adjunctive therapy for LE, owing to its potential to alleviate symptoms. Significant heterogeneity persists in clinical acupuncture protocols, and the optimal selection of acupoints remains inadequately defined, thereby limiting the generalizability and standardization of treatment approaches. Objective:This study seeks to identify and synthesize the most frequently used and effective acupoint combinations for LE treatment through the application of data mining techniques to analyze existing clinical literature. Methods:Eight electronic databases were searched for studies on acupuncture treatments for LE published from January 2015 to March 2025. Randomized controlled trials and clinical controlled trials that reported acupuncture prescriptions for LE were included. Reviews, meta-analyses, protocols, animal experiments, guidelines, and case reports were excluded. Literature screening and data extraction were performed based on predefined criteria. Microsoft Excel 2019 was used for descriptive statistical analysis. Association rule analysis was performed using R Version 4.5.0. A visual association network of acupoints was generated using Cytoscape 3.10.1. OriginPro2024 was used for clustering and correlation analyses of acupoints. Results:Forty-two articles were included according to the established criteria. Regarding treatment parameters, the most frequently used needle type was 0.30 mm × 40 mm, and a 30-minute retention time was predominant. The most common treatment frequency was 7 days per week, with total duration most commonly being 20 days. Regarding acupoint selection, the most frequently used acupoints were Ashi point, Quchi (LI11), Shousanli (LI10), Hegu (LI4) and Waiguan (TE5). The Large Intestine Meridian of Hand Yangming was the most frequently used meridian. Among specific point categories, five-shu points were the most frequently employed. The frequency of acupoints used was highest in the elbow. The association analysis showed the most supported acupoint pair was LI11 and LI10. Core acupoints identified for LE treatment included Ashi point, LI4, LI11, LI10 and TE5. By hierarchical cluster analysis, the acupuncture points were categorized into four categories. Conclusion:This review identified 0.30 mm × 40 mm needles, 30‑minute retention, 7 sessions weekly, and 20 days total duration as the predominant treatment parameters for lateral epicondylitis. The core acupoints included Ashi, LI11, LI10, LI4, and TE5. Notably, the combination of LI11 and LI10 constituted the most significant association rule. The Large Intestine Meridian and five‑shu points were most frequently used, and acupoint distribution was highest in the elbow. These findings provide evidence-based support for clinical acupuncture decision-making, yet further prospective trials are needed to confirm the validity of these patterns.

Background Endoscopic submucosal dissection (ESD) has been widely accepted as the gold standard curative approach for early oesophageal cancer and precancerous lesions. However, post-ESD oesophageal stricture remains a challenging clinical complication. In this study, a novel oesophageal catheter for stricture prevention after oesophageal ESD is introduced, and a preliminary description of the feasibility and safety of this new method are provided. Methods In total, five patients who underwent oesophageal ESD and received the novel oesophageal catheter for stricture prevention or a nasogastric tube between September 2024 and October 2025 at Jiangyin Hospital Affiliated to Nantong University were included. The patients’ clinical characteristics, endoscopic treatment and follow-up data were analysed. The primary outcome was the proportion of patients who developed oesophageal stricture after ESD. Secondary outcomes included the number of endoscopic balloon dilation or endoscopic radial incision (ERI) sessions required for stricture resolution and procedure-related adverse events. Results Four patients who underwent catheter placement immediately after ESD did not develop stricture during follow-up (average follow-up period: 17.25 months). The fifth patient with delayed catheter placement (due to concerns about pharyngeal pain symptoms) developed stricture, which was temporarily resolved by ERI but recurred six months later. Subsequent placement of the same catheter controlled the stricture, and this patient was followed up for 6 months after catheter removal. Conclusion The novel oesophageal catheter for stricture prevention represents potentially safe, low-cost and readily available prophylactic strategy for patients undergoing oesophageal ESD. This simple and accessible approach may offer preliminary insights for clinical practice, suggesting the possibility of reducing post-ESD stricture rates and improving long-term quality of life, although further validation in larger cohorts is needed. Trail registration This study was retrospectively registered at the Chinese Clinical Trial Registry site. [Registration number: ChiCTR2500112721(https://www.chictr.org.cn/); registration date: November 19, 2025].

目的 分析结直肠腺瘤患者血清胆汁酸谱的组成变化,初步探讨血清胆汁酸谱对结直肠腺瘤的诊断价值.方法 纳入2021年11月至2022年4月南通大学附属江阴医院诊断的55例结直肠腺瘤患者作为观察组,并选择同期40例健康体检人群作为对照组.应用液相色谱串联质谱法测定外周血16型胆汁酸水平,采用多因素Logistic回归分析结直肠腺瘤患病危险因素.将组间有差异的指标纳入二元Logistic回归方程,建立诊断模型.采用受试者工作特征曲线(ROC)评估单项胆汁酸指标及诊断模型的诊断效能.结果 观察组患者的脱氧胆酸(DCA)、甘氨脱氧胆酸(GDCA)、牛磺脱氧胆酸(TDCA)水平分别为140.9(124.7,157.0)ng/mL、69.0(58.2,75.0)ng/mL、16.0(10.4,24.4)ng/mL,明显高于对照组的126.2(114.0,145.8)ng/mL、59.0(55.0,71.7)ng/mL、9.3(5.3,18.4)ng/mL,差异均有统计学意义(P<0.05);经Logistic回归分析结果显示,DCA≥122.1 ng/mL、TDCA≥14.6 ng/mL诊断结直肠腺瘤的OR值分别为4.426(1.460～13.421)、2.777(1.065～7.240);经ROC曲线分析结果显示,TDCA在单项指标中诊断结直肠腺瘤价值最高[曲线下面积(AUC)为0.671,敏感性为61.8％,特异性为72.5％],联合应用DCA及TDCA的诊断模型诊断结直肠腺瘤的AUC为0.724,敏感性为96.4％,特异性为42.5％.结论 DCA、GDCA、TDCA在结直肠腺瘤患者中高表达;TDCA联合DCA组成的诊断模型对结直肠腺瘤具有较高的诊断敏感性,为结直肠腺瘤的血清学筛查提供了新的思路.

目的 分析窄带成像联合放大内镜诊断慢性萎缩性胃炎及肠上皮化生的临床价值.方法 选择该院2020年1-12月期间收治的疑似慢性胃炎患者60例.以上对象分别接受普通白光内镜检查、窄带成像联合放大内镜诊断.经内镜检查完成,所有患者接受病理化验,并将其作为临床检验的金标准,分析窄带成像联合放大内镜诊断的临床价值.结果 经诊断,该组患者普通白光内镜诊断萎缩性胃炎的准确率70.00％(42/60)、灵敏度78.95％(30/38)、特异性 54.55％(12/22)都较窄带成像联合放大内镜 91.67％(55/60)、94.74％(36/38)、86.36％(19/22)低(P＜0.05);该组患者窄带成像联合放大内镜诊断肠上皮化生的准确率93.33％(56/60)、灵敏度97.50％(39/40)、特异性 85.00％(17/20)也较普通白光内镜 68.33％(41/60)、77.50％(31/40)、50.00％(10/20)高,差异有统计学意义(P＜0.05).结论 针对慢性萎缩性胃炎及肠上皮化生患者,临床选择窄带成像联合放大内镜检查,具有较高的临床诊断价值,临床优势较高.

Background/Aims Endoscopic wound suturing is an important factor that affects the ability to remove large and full-thickness lesions during endoscopic resection. We aimed to evaluate the effect of a traction metal clip with a fishhook-like device on wound sutures after endoscopic resection. Methods From July 2020 to April 2021, patients who met the enrollment criteria were treated with a fishhook-like device during the operation to suture the postoperative wound (group A). Patients with similar conditions and similar size wounds who were treated with a “purse-string suture” to suture the wounds were retrospectively analyzed as the control group (group B). Difference in the suture rate, adverse events, time required for suturing, and number of metal clips were compared between the two groups. Results The time required for suturing was 7.72±0.51 minutes in group A and 11.50±0.91 minutes in group B. This difference was statistically significant (F=13.071, p=0.001). The number of metal clamps used in group A averaged 8.1 pieces/case, and the number of metal clamps used in group B averaged 7.3 pieces/case. This difference was not statistically significant (F=0.971, p=0.331). Conclusions The traction metal clip with the fishhook-like device is ingeniously designed and easy to operate. It has a good suture effect on the wound after endoscopic submucosal dissection and effectively prevents postoperative adverse events.

Objective The emergence of multi-drug resistance (MDR) in esophageal carcinoma has severely affected the effect of chemotherapy and shortened the survival of patients. To this end, we intend to develop a biomimetic nano-targeting drug modified by cancer cell membrane, and investigate its therapeutic effect. Methods The degradable poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) co-loaded with doxorubicin (DOX) and curcumin (Cur) were prepared by solvent evaporation method. TE10 cell membrane and Distearoyl phosphatidylethanolamine-polyethylene glycol (DSPE-PEG) were then coated on the PLGA NPs by membrane extrusion to prepare the PEG-TE10@PLGA@DOX-Cur NPs (PMPNs). Size and zeta potential of the PMPNs were analyzed by lazer particle analyzer, and the morphology of PMPNs was observed by transmission electron microscope. The TE10 cell membrane protein on PMPNs was analyzed by gel electrophoresis. The DOX-resistant esophageal cancer cell model TE10/DOX was established through high-dose induction. The In vitro homologous targeting ability of PMPNs was evaluated by cell uptake assay, and the in vitro anti-tumor effect of PMPNs was assessed through CCK-8, clone formation and flow cytometry. A Balb/c mouse model of TE10/DOX xenograft was constructed to evaluate the anti-tumor effect in vivo and the bio-safety of PMPNs. Results The prepared cell membrane coated PMPNs had a regular spherical structure with an average diameter of 177 nm. PMPNs could directly target TE10 and TE10/DOX cells or TE10/DOX xenografted tumor and effectively inhibit the growth of DOX-resistant esophageal carcinoma. Besides, the PMPNs was confirmed to have high biosafety. Conclusion In this study, a targeted biomimetic nano-drug delivery system PMPNs was successfully prepared, which overcome the MDR of esophageal carcinoma by co-delivering DOX and sensitizer curcumin.

PURPOSE:Esophageal cancer is one of the most common malignancies worldwide. Ubiquitin-dependent degradation of regulatory proteins reportedly plays a central role in diverse cellular processes. This study investigated the expression levels of ubiquitin in esophageal squamous cell carcinoma tissues and the functions of ubiquitin in the context of esophageal squamous cell carcinoma progression.METHODS:The expression of ubiquitin in esophageal squamous cell carcinoma and normal esophageal samples was determined via immunohistochemistry. Serum ubiquitin levels were determined by enzyme-linked immunosorbent assay. The association between serum ubiquitin level and clinicopathological factors was analyzed. Real-time PCR analysis was employed to measure the mRNA levels of the ubiquitin coding genes ubiquitin B and ubiquitin C. Proliferation assays, colony formation assays, and Transwell-based assays were used to determine the influence of ubiquitin on cell growth and cell invasion. Proteomic analysis was performed to identify the proteins associated with ubiquitin.RESULTS:Ubiquitin expression in esophageal squamous cell carcinoma tissues was markedly higher than that in normal and tumor adjacent tissues. The levels of ubiquitin in esophageal squamous cell carcinoma serum samples were significantly higher than those in healthy controls. Serum ubiquitin levels were correlated with tumor stage and lymph node metastasis. To silence the expression of ubiquitin, we knocked down the ubiquitin coding genes ubiquitin B and ubiquitin C in TE-1 and Eca-109 cells. Silencing ubiquitin resulted in the suppression of cell growth, chemoresistance, colony formation and cell migration in esophageal squamous cell carcinoma cells. Proteomic analysis in esophageal squamous cell carcinoma cells showed that knockdown of ubiquitin coding genes deregulated the expression of 159 proteins (92 were upregulated and 67 were downregulated) involved in multiple pathways. These proteins included ferritin light chain, ferritin heavy chain, cellular retinoic acid-binding protein 2, and DNA replication factor 1.CONCLUSION:Ubiquitin expression is upregulated in esophageal squamous cell carcinoma tissues and serum samples. Serum ubiquitin levels were correlated with tumor stage and lymph node metastasis. Downregulation of ubiquitin suppresses the aggressive phenotypes of esophageal squamous cell carcinoma cells by complex mechanisms; ubiquitin may represent a novel target for the treatment of esophageal squamous cell carcinoma.

目的:研究血清胰蛋白酶原2(TAT-2)、胃蛋白酶原(PG)和高尔基体蛋白(GP73)单独和联合检测对早期胃癌和胃癌前病变的诊断价值.方法:165例胃部不适患者根据内镜和病理诊断结果,分为胃癌前病变组49例,早期胃癌组36例,进展期胃癌组80例,另选90例活检为正常胃黏膜或慢性浅表性胃炎作为对照组,采用时间分辨荧光免疫法(TRFIA)检测血清TAT-2、PG和GP73水平,运用ROC曲线和logistic回归评价对早期胃癌和胃癌前病变的诊断效果.结果:与对照组相比,胃癌前病变组、早期胃癌组和进展期胃癌组血清TAT-2、GP73水平较高,胃癌前病变组、早期胃癌组PGⅠ、PGR(PGⅠ/PGⅡ)较高,进展期胃癌组较低(P<0.05),PGⅡ组间差异无统计学意义.TAT-2、PGⅠ、PGR和GP73单独和联合诊断胃癌前病变的ROC曲线下面积分别为0.743、0.676、0.810、0.824和0.925,最大Youden指数分别为0.43、0.36、0.62、0.65和0.67;四项指标单独和联合诊断早期胃癌的ROC曲线下面积分别为0.641、0.730、0.857、0.908和0.950,最大Youden指数分别为0.32、0.41、0.69、0.74和0.78;四项指标单独和联合诊断进展期胃癌的ROC曲线下面积分别为0.727、0.694、0.900、0.911和0.976,最大Youden指数分别为0.42、0.40、0.68、0.70和0.86,联合检测曲线下面积大于单独检测.结论:TAT-2、PGⅠ、PGR和GP73单独检测对早期胃癌、胃癌前病变和胃癌具有一定的诊断价值,而联合检测具有较高的临床诊断价值.

目的 泛素-蛋白酶系统(UPS)在食管癌的发生、发展中具有重要作用.泛素分子(UB)作为UPS的执行者,在食管癌发病过程中的表达情况及其临床诊断价值鲜有报道.文中探讨血清UB在食管鳞癌(ESCC)中的表达及其诊断价值.方法 纳入2018年2月至2019年5月南通大学附属江阴医院收治的88例ESCC患者(ESCC组)及40例进行食管癌筛查无异常者(正常对照组).采用ELISA法测定血清UB,化学发光法测定血清鳞状细胞癌抗原(SCC)和癌胚抗原(CEA).采用ROC曲线分析各指标诊断价值,并分析血清UB与临床病理特征的关系. 结果 与正常对照组相比,ESCC组患者血清UB水平显著升高[(41.96±3.273) ng/mL vs(80.86±7.993) ng/mL,P＜0.05].在有无淋巴转移及肿瘤不同分期患者中,血清UB表达差异有统计学意义(P＜0.05).UB、SCC、CEA以及三者联合诊断ESCC的灵敏度分别为65.9％、52.3％、51.1％、76.1％;ROC曲线下面积分别为:0.690、0.667、0.635、0.795. 结论 血清UB在ESCC中高表达,且与肿瘤进展密切相关.联合血清UB与SCC、CEA可提高诊断ESCC的灵敏度,并可作为有效的血清学筛查指标.

背景:我国急性胰腺炎(AP)发病率逐年上升,病死率居高不下,探索可用于判断AP病情严重程度的血液生化指标对AP的诊断和治疗具有重要的临床意义.目的:探讨血清胰蛋白酶原-2(TAT-2)和血细胞比容(HCT)在AP病情严重程度中的预测价值.方法:收集2016年9月—2018年6月江阴市人民医院收治的175例AP患者,根据病情严重程度分为轻度急性胰腺炎(MAP)组、中重度急性胰腺炎(MSAP)组、重度急性胰腺炎(SAP)组,并选取同期健康体检者作为对照组.测定血清TAT-2、HCT,并行BISAP评分、APACHEⅡ评分、Balthazar CT评分.采用ROC曲线分析各指标诊断SAP的价值.结果:四组间TAT-2、HCT、BISAP评分、APACHEⅡ评分、Balthazar CT评分相比差异有统计学意义(P<0.05).SAP组TAT-2、HCT明显高于MAP组、MSAP组、对照组(P<0.05),MSAP组TAT-2、HCT明显高于MAP组、对照组(P<0.05),MAP组TAT-2、HCT显著高于对照组(P<0.05).ROC曲线分析示,TAT-2、HCT以及两者联合预测SAP的敏感性分别为69.7％、84.7％、88.5％,特异性分别为95.3％、67.8％、65.1％,AUC分别为0.858、0.783、0.875.结论:TAT-2、HCT可有效评估AP病情的严重程度,两者联合检测更有助于进一步预测SAP的发生,对于AP的治疗起有重要指导作用.

According to CSA diagnostic criteria, inclusion criteria and exclusion criteria, the qualified CSA subjects are determined as research objects, which were randomly divided into treatment group and control group; treatment group adopts the laser needle-knife combined with supine restoration massotherapy method, while the control group only adopts supine restoration therapy.In the treatment group, He-Ne laser radiation was performed for 30 minutes after the needle was released, 1 times every 10 days, 3 times for a course, 2 courses of treatment in total, which is combined with supine restoration massotherapy for 20 minutes, 3 times a week, 10 times for 1 course, 2 courses of treatment in total.In control group, supine restoration massotherapy was only adopted for 20 minutes of treatment, 3 times a week, 10 times for 1 course of treatment and 2 courses of treatment in total.Color Doppler ultrasound was used to respectively observe the patients from two groups before treatment and on the sixtieth day after treatment for the changes in vertebral artery diameter (D) of C5-6 segment of bilateral vertebral artery, systolic peak velocity (PSV), end diastolic velocity (EDV), resistance index (RI) and pulsatility index (PI), so as to analyze the effects of laser needle-knife combined with supine recumbent massage on vertebral artery morphology and hemodynamics in patients.Results: There was significant difference between the improvement of PSV before and after treatment in the vertebral artery C5-6 segment and the control group (P <0.05), compared with the control group, the EDV improvement was statistically significant, PI was significantly improved before and after treatment, compared with the control group, it was statistically significant (P <0.05).Conclusions: The vertebral artery diameter (D) hemodynamics index (PSV, EDV, RI, PI) in vertebral artery C5-6 segment of patients with CSA can be improved by laser needle-knife compound therapy to varying degrees.The clinical study registration number is No.2013临审第 (03) 号.

Esophageal squamous cell carcinoma (ESCC) has become one of the most common causes of cancer‑associated mortality worldwide. Transforming growth factor‑activated kinase (TAK1)‑binding protein 3 (TAB3) is essential for activation of the NF (NF)‑κB pathway in response to TAK1 activation. The NF‑κB pathway serves important roles in tumor cell proliferation and migration; however, the clinical relevance of TAB3 and its biological function in ESCC progression remain elusive. The present study investigated the expression and function of TAB3 in ESCC tissues, and its association with the clinical prognosis of patients. The results demonstrated that TAB3 expression was significantly increased in human ESCC cell lines and tissue samples, and the expression of TAB3 was associated with ESCC lymph node metastasis, T stage, pathological grade and Ki‑67 expression in 80 ESCC samples, as determined by immunohistochemistry. Patients with ESCC and high TAB3 expression exhibited worse overall survival. Furthermore, knockdown of TAB3 by small interfering RNA inhibited the proliferation of ESCC cells, and reduced the migration and invasion of ESCC cells. In addition, knockdown of TAB3 decreased the expression of the NF‑κB pathway in TE‑1 cells. Taken together, these results demonstrated that TAB3 may be a promising therapeutic target for the treatment of ESCC.

结肠癌为威胁我国人民健康的十大恶性肿瘤之一,且呈上升趋势,而直肠、乙状结肠部癌的发生率最高. 早期发现、切除结肠腺瘤等癌前病变,有助于降低结肠、直肠癌的发病率和死亡率[1]. ESD可完整剥离黏膜病变,对黏膜内癌的治疗效果与外科手术相当,且创伤小、恢复快、费用低,目前已成为结肠癌前病变、黏膜内癌的重要治疗方法[1-2]. 与EMR相比,ESD切除病变范围更大,切除标本更有助于精确的病理评估,但ESD技术操作难度增加,并发症风险增加.肠道管壁较食管、胃更薄,且肠道走行不固定,导致肠道ESD操作更为困难[2-3]. 因此,有多项研究通过辅助牵引技术的应用,以改善 ESD 操作视野,降低难度,取得了较好的效果[2-7]. 但针对直肠、乙状结肠ESD的体外牵引技术报道很少. 本研究对体外异物钳牵引法在直肠、乙状结肠ESD中的辅助作用进行了探讨,现总结报道如下.

Background:Epstein-Barr virus (EBV)is associated with various human lymphoid and epithelial malignancies such as Burkitt's lymphoma,nasopharyngeal carcinoma and gastric carcinoma. LMP2A,a virus-encoded latent membrane protein is expressed in a portion of EBV-associated gastric carcinoma (EBVaGC)and has been shown to be related with the tumorigenesis and progression of EBVaGC. Aims:To explore the effect of LMP2A silencing on growth of EBVaGC cells in vitro by using a lentivirus-mediated RNA interference (RNAi)to inhibit LMP2A gene expression. Methods:A lentivirus vector pGCSIL-LMP2A-shRNA-LV and a negative control vector were constructed and transfected into the EBVaGC cell line GT38. Real-time PCR and Western blotting were used to determine the inhibitory effect of the lentivirus vector;CCK-8 assay,colony formation assay and flow cytometry were employed to assess the cell growth,cell cycle and apoptosis of GT38 cells. Results:In GT38 cells transfected with LMP2A-shRNA-LV,the expression level of LMP2A mRNA was decreased by 65. 4%,and that of LMP2A protein was reduced by 50. 8%;the cell proliferation was inhibited,the colony formation ability was suppressed,the percentage of cells in G0 / G1 phase and apoptotic rate were increased when compared with those transfected with negative control vector or without transfection (P < 0. 05). Conclusions:Lentivirus-mediated RNAi is effective for silencing LMP2A gene expression,subsequently inhibiting the growth of EBVaGC cells,inducing G0 / G1 phase arrest and enhancing cell apoptosis in vitro. LMP2A is supposed to be a potential target for gene therapy of EBVaGC.

In this study, lentivirus-mediated RNA interference (RNAi) was applied to inhibit latent membrane protein 2A (LMP2A) gene expression, in order to explore the effects of LMP2A silencing on the growth of an Epstein-Barr virus-associated gastric carcinoma (EBVaGC) cell line in vitro. Lentivirus-mediated RNAi technology was employed to specifically knock down the LMP2A gene in the EBV-positive gastric carcinoma cell line GT38. After infection, reverse transcription-quantitative polymerase chain reaction, western blotting, flow cytometry and colony formation assays were conducted to evaluate the expression of LMP2A and the biological behavior of the GT38 cell line in vitro. The results showed that the expression of the LMP2A gene was clearly downregulated in the infected cells, which indicated that a highly efficient and stable lentivirus vector was successfully constructed. In the GT38 cells in which the expression of LMP2A was downregulated, the proliferation and colony formation of the cells was significantly inhibited. In addition, it was found that the cell cycle of the GT38 cells was arrested in the G0/G1 phase and the apoptosis rate was increased. These results indicate that lentivirus-mediated RNAi knockdown of LMP2A inhibits the growth of the EBVaGC cell line GT38 in vitro, and suggests that LMP2A is a potential target for gene therapy in the treatment of EBVaGC.

Objective: To discuss and explore the clinical effect of the treatment of 45 cases of primary dysmenorrhea (PD) by a combination of laser acupuncture with manipulation. Method: 90 cases of patient with PD were randomly divided into the treatment group and control group. The treatment group was treated by a combination of laser acupuncture with manipulation, the control group was treated by a manipulation therapy, the patients in both groups were analyzed in terms of clinical effect. Result: the post-treatment overall effective rate of treatment group was 93.33%, the control group, 82.22%; and the difference in VAS score between the two groups was statistically significant. Conclusion: using laser acupuncture to treat the patients with PD can improve the patient's clinical symptoms significantly, and it does a better job in treating part of the symptoms than the manipulation therapy alone, it is a safe and effective therapy. Dysmenorrhea is a common disease that is encountered in gynecological clinic. The incidence rate of dysmenorrhea in women in China is 33.19%, among the female college students, its incidence rate can be up to 56.4%, and primary dysmenorrhea (PD) accounts for 36.06% of overall incidence rate[11. The author treated the PD with laser acupuncture combined with manipulation and achieved a good curative effect, the process of which is now reported as follows:

目的 观察急诊胃镜下止血联合奥美拉唑治疗上消化道溃疡出血患者的临床疗效.方法 选取2015年4月-2017年4月在医院进行治疗的上消化道溃疡出血患者94例,随机分为观察组和对照组,每组47例.对照组患者在常规药物止血治疗的基础上给予奥美拉唑40 mg静脉滴注,持续7 d;观察组患者在对照组治疗的基础上加用急诊胃镜下止血治疗.评价2组患者的疗效,并统计其再出血率和转手术率.结果 治疗后观察组患者疗效评价总有效率为93.62%(44/47)高于对照组的78.72%(37/47),差异有统计学意义(χ2=4.3741,P<0.05).观察组患者再出血率和转手术率均低于对照组,差异均有统计学意义(P<0.05).结论 急诊胃镜下止血联合奥美拉唑治疗上消化道溃疡出血患者不仅具有显著的治疗效果,而且再出血率和转手术率均较低,安全性高,值得临床上进一步推广应用.

Cancer stem-like cells (CSCs) have been reported to play major roles in tumorigenesis, tumor relapse, and metastasis after therapy against colorectal carcinoma (CRC). Therefore, identification of colorectal CSC regulators could provide promising targets for CRC. Ligand-of-Numb protein X1 (LNX1) is one E3 ubiquitin ligase which mediates the ubiquitination and degradation of Numb. Although several studies indicate LNX1 could be a potential suppressor of cancer diseases, the functions of LNX1 in mediating cancer stemness remain poorly understood. In this study, LNX1 was identified as a negative regulator of cancer stemness in CRC, which was downregulated in colonospheres or side population (SP) cells. Furthermore, the coxsackievirus and adenovirus receptor (CXADR) was found to be one critical downstream mediator of cancer stemness regulated by LNX1. Interestingly, the anti-breast cancer drug tamoxifen was found to be an agonist of LNX1 and suppress cancer stemness in CRC. In sum, this study provided the evidences that LNX1 signaling plays important roles in regulating the stemness of colon cancer cells.

Objective: To investigate the effectiveness of combined laser needle-knife and massage on cervical spondylotic vertebral arteriopathy patients. Summary of background data: With the recent rise of electronic businesses, the incidence of cervical spondylosis has also risen rapidly. Methods: Cervical spondylotic vertebral arteriopathy patients were treated using laser needle-knife with massage, and compared to patients who only received the massage. A 3D anatomical and hemodynamic model was developed. Results: The symptomatic and functional overall scores were reduced by 71.43% after the combined treatment. Results from the finite element analysis indicated that the maximum flow rate of the left vertebral artery was improved by 47.52% and the right was improved by 38.67%. Conclusion: A combined treatment of cervical spondylotic vertebral arteriopathy using laser needle-knife and massage is an effective approach with a therapeutic mechanism closely related to hemodynamics.