Objectives To evaluate the performance characteristics of the new real-time PCR COBAS Taqman 48 analyzer.Methods According to the evaluation protocols of National Committee for Clinical Laboratory Standards(NCCLS), COBAS Taqman was assessed by tests of analytical precision,carryover,calibration verification and reference range.Results The assay showed low intra-assay (6.9%～8.6%),interassay (10.5%～18.7%) and total(10.8%～20.2%)variations, with good linearity and correlation with expected HBV DNA titers over a wide range (6.0 to 107 IU/ml).Compared with another analyzer, results among clinical specimens yielded good correlation(r2=0.998).Conclusion The performance characteristics of the COBAS Taqman HBV DNA test demonstrated that it provides a sensitive and reliable method for quantitation of HBV DNA levels in the routine molecular laboratory.Through this validation,a set of explicit nucleic acid evaluation protocols has been established in favor of the implementation of the clinical laboratory standardization operation.

Objective To describe the clinicopathological behavior of oxyphil cell adenoma of parotid and analyze their immunophenotypes and morphological features.Methods With review of the literatures,histopathology,clinical data and immunohistochemistry was used to investigate the clinical appearance,pathological features and differential diagnoses in 12 cases with oxyphil cell adenoma of parotid.Results The tumor cells were oval or polygonal,with abundant,eosinophilic cytoplasm.Immunohistochemistry showed reactivity for EMA,CK,but negative for Vimentin,Actin,S-100 in tumor cells.Conclusions Oxyphil cell adenoma of parotid is a rare type tumor.The definite diagnosis of this disease depends on pathological examination,it should be differentiated from other parotid neoplasms.

Objective To study the relations between the expressions of GLUT1 to the occurrence,development or biology behaviors of human oral squamous cell carcinoma(OSCC).Methods Immunohistochemistry S-P method was used to detect the expression of GLUT1 in 52 cases of OSCC tissues,the tissues of atypical hyperplasia of tumor-adjacent and normal oral mucosa.Results The positive signal of GLUT1 protein was located in cell membrane,the positive rate of its expressions was high,low and lower respectively in the tumor tissues,the tissues of atypical hyperplasia of tumor-adjacent and normal oral mucosa(88.46%,54.84% and 19.23%).There was a significant difference in the expressions of GLUT1 among the three groups(P<0.01).Conclusion The GLUT1 protein might serve as a significant predicting factor for proliferative status of carcinoma cell.

Objective To study the relations between cellular biologic behavior of human oral squamous cell carcinoma(OSCC)and the expressions of IGF-Ⅰ.Methods The expressions of IGF-Ⅰ were analyzed in 52 cases with OSCC by immunohistochemistry method(S-P method).Results The positive signal of IGF-Ⅰ was located in cytoplasm,the increased expressions of IGF-Ⅰ were related to the development and extent of OSCC.The expressions of IGF-Ⅰ were related to the histological grades and clinical stages.Conclusions The expressions of IGF-Ⅰ in OSCC might be closely related with the invasion and lymph metastasis.IGF-Ⅰ could be used in diagnosis,therapy and prognosis as an assistant marker.

Objective To explore the relationship between expressions of COX-2 and HIF-1α and progression as well as metastases of oral squamous cell carcinoma. Methods The expressions of COX-2 and HIF-1α were detected in 52 oral squamous cell carcinoma patients by immunohistochemical staining.The relationship between the expressions of COX-2 and HIF-1α and progression as well as metastases of oral squamous cell carcinoma was evaluated.Results Among the 52 cases with oral squamous cell carcinoma,31 cases were positive for COX-2(59.6%),and 37 cases for HIF-1α(71.2%) respectively.The expressions of COX-2 and HIF-1α were closely correlated with stage and lymph node metastases of oral squamous cell carcinoma(P<0.05).Conclusions COX-2 and HIF-1α might take part in progression and metastases of oral squamous cell carcinoma.The expressions of COX-2 and HIF-1α would be helpful to the diagnosis and treatment of oral squamous cell carcinoma.

OBJECTIVE:To analyze the correlation of hepatitis B virus (HBV) DNA with the serological markers of HB in the serum of chronic HB patients after treatment PCR method and to analyze the status of these markers and the multiplication of virus.METHODS:Peripheral blood samples were collected from 480 chronic HB patients, aged 15 - 50, who had been treated by anti-nucleotide drugs or traditional Chinese herbs and showed normal ALT/AST. Both COBAS AMPLICOR HBV MONIORTM kit (internal-standard PCR method) and Light Cycler real time fluorescent quantitative PCR instrument (external-quantitative standard PCR method) were used to measure the HBV DNAS level. 42 of the 312 patients with the HBV DNA level lower than the minimum test limit measured by COBAS AMPLICOR HBV MONIORTM kit and HBeAg positive (>4 S/CO) underwent microparticle enzyme immunoassay (MEIA) to test the HBsAg, anti-HBs, HBeAg, anti-HBe, and HBcIgG.RESULTS:Seven of the 42 patients with HBV DNA negative measured by COBAS AMPLICOR HBV MONIORTM kit lower then the minimum test limit were shown as HBV DNA positive by Light Cycler real time fluorescent quantitative PCR. The 42 patients were HBsAg (+), anti-HBs (-), HBeAg (+), anti-HBe (-), and anti-HBcIgG (-), with an average HBeAg level of 42.26 S/CO and a positive HBeAg rate of 13.46%.CONCLUSION:HBeAg positivity does not necessarily means an active multiplication of HBV. The changes of the serological markers of HBV may be not consistent with that of HBV DNA. It is more objective to undergo both internal-standard and external-quantitative standard methods.

牙髓病和根尖周病是口腔科常见病,为避免在治疗时给病人带来的痛苦,在治疗前一般先采用止痛措施.我们根据该病变的临床及组织病理学特点和冷冻疗法的作用机理,将成熟的冷冻术应用于牙髓病和根尖周病的治疗过程中,通过临床实践,取得良好的效果,下面是研究过程及结果:

临床上可遇到因使用皮圈牵引不当而引起前牙脱位,甚至最终脱落的患者,近年来也常有此类文献报道.其主要原因是用橡皮管剪成皮圈单独套入上中切牙,以关闭正中间隙,而皮圈滑入牙根尖,致使牙槽骨受压吸收,牙齿逐渐伸长,造成脱位甚至脱落.有关专家早就提出禁止单独使用皮圈来关闭牙间隙,以防发生不良后果,但仍有此类事故发生.近年来,我科尝试联合外科进行脱位牙齿的保存治疗,取得较好的疗效.

复发性口疮是口腔黏膜病中患病率最高的一种慢性病,以口腔黏膜上出现周期性反复发作、有较强烈疼痛和自限性溃疡为特点.笔者近十年来将该病分为五型,以六味地黄丸为治疗基本方,根据临床表现进行加减,配合"AT"药膜(由洋金花提取液、甲硝唑、地塞米松等药物配制而成)局部用药及西医改善微循环抗炎治疗,取得较好疗效,现介绍如下.

目的了解开封市12岁儿童恒牙龋发病情况.方法按照全国第二次口腔流行病学调查标准对随机抽取的1033名12岁儿童进行检查并记录.结果 12岁儿童恒牙患龋率为19.65%,龋均为0.32,已达到我国2000年口腔健康目标的要求.恒牙龋中,牙牙合面患龋率显著高于其他牙面,第一磨牙患龋率最高.结论儿童口腔保健知识普及较好,但有待于进一步加强.应重视对年轻恒牙的保护,建议推广应用窝沟封闭防龋并提高龋齿的充填率.