The study aimed to develop aneuploid lines of tetraploid wheat (Triticum turgidum var durum, AABB) to provide genetic materials to immunologists in our project for understanding the precise role of gliadins in the induction of Celiac disease occurring in genetically susceptible individuals. The genes for gliadins are coded at gli loci located on the short arms of chromosome group 1 (1AS, 1BS and 1DS) and 6 (6AS, 6BS and 6DS) in hexaploid wheat. An interspecific crossing program was implemented with four hexaploid cytogenetic stocks as female parents crossed with the Indian durum wheat variety HI8663 as the male parent. Molecular marker (EST-SSR) based screening was employed to identify plants in BC1F2 lacking specific chromosomes. The identified plants were selfed and the progenies were reconfirmed for the absence of 1A/1AS or 6A/6AS. Multiplex SSR and gene based markers were employed to ascertain the presence of D chromosomes. The study resulted in the development of nine durum aneuploid lines lacking whole chromosome 1A, three lacking short arm of chromosome 1A, five lacking whole chromosome 6A and four aneuploid lines lacking short arm of chromosome 6A. Interestingly, all the developed lines lacking chromosome 1A or its short arm possessed chromosome 1D. In a few aneuploid lines, one or more chromosomes of D genome were retained. The aneuploid cytogenetic stocks lacking specific chromosomes developed in the present study can be used for further investigation into the role of celiac epitopes present on chromosomes 1A and 6A.

India has increased its wheat production phenomenally in the last two decades and it now has a buffer stock of 9.7 million tonnes. However, despite the release of several wheat cultivars, the end-use quality traits of Indian wheat varieties have not been explored in-depth to determine the increasing demand of the domestic processing industry as well as export. In this study, 55 wheat genotypes including 47 released varieties, and 8 genetic stocks were grown along with 10 Australian varieties grown during cropping seasons: 2019–2020 and 2020–2021 and diversity in different physiochemical and rheological traits was evaluated. They showed considerable diversity in all the quality traits studied. However, very few genotypes could be found suitable for any one end-use. Five genotypes were found to possess four to five traits for superior bread-making quality. Two varieties and three advanced breeding lines had up to four good chapati quality traits. None of the released varieties investigated had suitable traits for biscuit making; however, two breeding lines possessed requisite quality traits suitable for biscuit making. It is, therefore, concluded that systematic breeding efforts are required to develop genotypes that bring together the most important quality traits in a single genotype to be suitable for domestic industry as well as for export.

Understanding the impact of genotype, environment and their interaction on the expression of quality attributes aids in precise selection for improving wheat quality in breeding programmes. The present study analyzed 41 diverse wheat genotypes grown at three different environments during rabi 2019-20 for 15 quality traits. Location Indore showed high genotypic performance for most of the traits, followed by Delhi, indicating favorable environments for quality trait expression. AMMI and pooled ANOVA analyses revealed significant E+GEI effects for Fe (89.14%), Zn (87.68%), test weight (76.97%), and grain protein content (75.43%). Polyphenol oxidase activity (87.42%) and sedimentation value (66.35%) showed strong genotypic effects, highlighting substantial genetic diversity influencing these traits. GGE biplot analysis identified C306 (G34), C273 (G38), C518 (G39), and C591 (G40) as the best-performing and stable genotypes across locations for grain protein content, gluten components traits, Fe, Zn, and grain hardness. AEC view of GGE biplot highlighted ideal genotypes C273 (G38) and C518 (G39) for falling number, GW322 (G35) and C518 (G39) for damaged starch, and C306 (G34), CS46 (G10), and C591 (G40) for total sugars. C273 (G38), C518 (G39), C591 (G40), and C306 (G34) were identified as highly desirable for multiple quality traits, showcasing their value as parents for simultaneous improvement in wheat breeding programme

Biotechnology has a significant role in transforming horticultural crops, offering innovative solutions to enhance crop productivity, quality, and sustainability. One of the key contributions of biotechnology in horticulture is the development of efficient micropropagation techniques. This method allows for the rapid clonal propagation of elite horticultural varieties, ensuring the production of disease-free and genetically uniform plants on a large scale. Genetic improvement through biotechnology is another significant aspect of horticultural crop development. Techniques such as genetic engineering and marker-assisted breeding enable the incorporation of desirable traits into horticultural crops. These traits may include enhanced disease resistance, extended shelf life, improved flavour, and increased nutritional content, ultimately benefitting both growers and consumers. Biotechnology has facilitated the creation of transgenic horticultural crops with specific genetic modifications, conferring resistance to pests, diseases, and adverse environmental conditions. Overall, the role of biotechnology in horticultural crops is integral to advancing agricultural practices, improving crop productivity and quality, and contributing to sustainable and environmentally conscious approaches in horticulture.

The study of polymorphism of glutenin makes it possible to identify and isolate desirable genotypes with higher grain quality. In the last few years, only a part of the genetic diversity among the modern and popular wheat germplasm and varieties based on the polymorphism of glutenin subunits are captured. To address this 107 wheat varieties released across different agricultural zones in India, were used to investigate HMW-GS and LMW-GS allele polymorphism, gene diversity and genetic variation in the Glu-1 and Glu-3 loci. Among the different HMW-GS, the highest genetic variation was observed at the Glu-D1 locus with both Glu-D1a and Glu-D1d possessing genetic variation of 0.490, 0.484 respectively. The highest genetic variation at the Glu-A3 locus was observed at the Glu-A3c and GluA3b possessing a genetic variation of 0.463, 0.411 respectively. This was followed by the Glu-B3j having a genetic variation of 0.386 at the Glu-B3 locus. Over 20 years a remarkable increase in the Glu-D1d allele is observed in the newly released varieties in India. Among all the zones, Glu-A1-null is the least frequent allele at the Glu-1 locus, however, it is present as the predominant allele in the NHZ of India. This study elucidates the relationships of these HMW and LMW allelic frequencies and genetic variation with their geographical distribution over the two different periods. This study provides reference data that can be used to assist the breeding, quality evaluation and development of good-quality wheat varieties.

Background and Purpose: Myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) is increasingly recognized in children. Some children have isolated disease while others relapse. The study evaluates clinical, demographic and imaging features children with positive anti-MOG antibodies comparing to previously reported findings and correlate patterns on MR imaging with a relapsing course in MOGAD.Material and methods: All pediatrics patients with serum anti-MOG antibodies were reviewed. Demographic, clinical, and imaging data were evaluated. Patients with a relapsing course were compared to those with a single event. We assessed initial MR images of the brain, orbits and spine obtained at the onset of clinical symptoms, whether performed at our institution or elsewhere.Results: Thirty patients were included, fourteen with a single event and sixteen with more than one clinical event. The mean age was 8.1 years, with a mean follow-up of 58 months (range of 0.67 to 238 months). The relapsing patients had a mean of 3.5 relapses (range 2-12). 55% of patients had long segment optic nerve lesions, 53% of patients had cortical or peripheral white matter lesions, and 46% of patients had thalamic lesions. 43% of patients had spinal cord lesions, with 39% involving the central cord and 26% with long segment involvement. The imaging features between the groups were not statistically significant.Conclusion: There were no distinguishing features in relapsing versus non-relapsing patients. In the absence of any predictive characteristics for future relapse, patients should have regular clinical and imaging follow up.& COPY; 2022 Elsevier Masson SAS. All rights reserved.

Polyphenol oxidase (PPO) activity is a primary cause of the development of unattractive dark brown discoloration of wheat-based end products. The present study aims to evaluate a set of 41 diverse wheat genotypes grown at three different locations in India for grain phenol color reaction, PPO activity and molecular marker-based characterization of alleles of PPO genes. Relationships among these parameters were analyzed along with the effects of grain PPO activity on dough and chapati color at different time intervals. The mean PPO activity ranged from 7.42 to 27.57 min−1 g−1 10−3 among the genotypes and it showed a significant negative correlation with color brightness (L*) of dough rested for 0 min (r = -0.406), 15 min (r = -0.406), 2 h (r = -0.502) and 4 h (r = -0.551) and whole wheat flour-derived chapati rested for 2 h (r = -0.267) and 4 h (r = -0.424). The overall quality color score was negatively correlated with PPO activity (r = -0.863) and showed a positive correlation with both dough and chapati visual color measured at different time intervals. PPO activity in the genotypes carrying different alleles was found to be Ppo-A1a>Ppo-A1b; Ppo-B2d>Ppo-B2a; and Ppo-D1b>Ppo-D1a. The allelic constitution Ppo-A1bPpo-B2aPpo-D1a and Ppo-A1bPpo-B2dPpo-D1 was found to produce the lowest PPO activity, and thus these alleles are recommended to be used in marker assisted breeding for low PPO activity genotypes to minimize the discoloration of wheat-based end-products.

Celiac disease (CD) is a gluten related disorder which affects all age-groups and occurs in genetically susceptible population after introduction of gluten in diet. The worldwide prevalence of CD is ~1% and it is higher in certain "at-risk groups". The clinical features are variable, ranging from classical diarrhea to an asymptomatic state. Diagnosis requires serology and duodenal histology although a non-biopsy diagnosis is recommended by European Society of Pediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) for a select group of children. Treatment of CD is with a life-long strict gluten free diet (GFD) along with correction of nutritional deficiencies. Regular follow-up to assess compliance and efficacy of GFD is mandatory. Non-responsive CD needs evaluation by a specialist as it can be due to incorrect diagnosis, poor dietary compliance, coexisting conditions like small bowel bacterial overgrowth, pancreatic insufficiency etc. and lastly, refractory CD. Most patients diagnosed as CD in childhood receive no medical or dietary supervision after transition to adulthood and nearly a third are non-compliant to GFD. No requirement of medications, patient's perception of understanding GFD and absence of symptoms with intermittent non-compliance leads to neglect of care after transition. Poor dietary adherence leads to nutritional deficiencies, osteoporosis, fertility issues and risk of malignancy. It is mandatory that the patients know about CD, need of strict GFD, regular follow-up, disease complications, and are capable of communicating with the health-care personnel before transition. Formulating a phased transition care program with joint pediatric and adult clinics is required for a successful transition and improving the long-term outcome.

Background and Aims Crohn's disease [CD] and intestinal tuberculosis [ITB] are often difficult to differentiate. Mesenteric fat hypertrophy is a feature of CD. We evaluated the utility of fat indices (visceral fat [VF] and subcutaneous fat [SF]) in differentiating CD and ITB in children. Methods Symptomatic children diagnosed to have CD or ITB based on recommended criteria were enrolled. Clinical, anthropometric, and laboratory details were noted. Abdominal fat was measured on computed tomography in supine position at the level of L4 vertebrae. VF and SF area was measured separately by a radiologist, blinded to the diagnosis. The sum of VF and SF was taken as total fat [TF]. VF/SF and VF/TF ratios were calculated. Results Thirty-four (age 14 years [10.8-17.0], 14 boys) children were recruited: 12 had CD [seven boys, age 13.0 years] and 22 had ITB [seven boys, age 14.5 years]. VF area was higher in CD compared to ITB (18.34 cm(2) [15.62-40.01] vs 6.48 cm(2) [2.65-21.96]; p = 0.012). The SF and TF area was similar in ITB and CD. The ratios of VF/SF (0.82 [0.57-1.5] vs 0.33 [0.16-0.48]; p = 0.004) and VF/TF (0.45 [0.36-0.60] vs 0.25 [0.13-0.32]; p = 0.004) were significantly higher in CD. On comparing CD and ITB in boys and girls separately, the difference was significant for boys but not for girls. A VF/SF ratio of 0.609 predicted CD with a good sensitivity [75%] and specificity [86.4%] [area under the curve 0.795, 95% confidence interval 0.636-0.955; p = 0.005]. Conclusion The VF/SF ratio is a simple, non-invasive, objective parameter to differentiate CD and ITB in children, particularly boys. Larger studies are needed to validate this in girls.

The present study is the analysis of large scale data (31949 ha area and 79873 farmers) generated through the CFLD on pulses across the major pulses growing states under the ICAR-ATARIs of Kanpur (Uttar Pradesh), Jodhpur (Rajasthan), Pune (Maharashtra), Patna (Bihar), Jabalpur (Madhya Pradesh), Kolkata (West Bengal), Guwahati (Assam), Shillong (Meghalaya), Hyderabad (Andhra Pradesh), Bengaluru (Karnataka) and Patna (Bihar). The pulse crops included in this analysis were from all three growing seasons: kharif (pigeonpea-5556 ha, blackgram-6067 ha, and greengram-2689 ha), rabi (chickpea-8376 ha, lentil-3747 ha and field pea-1890 ha), and summer (greengram-3624 ha). The average performance data of CFLD were obtained for the above states for all the crops representing all three growing seasons during the cropping seasons of 2016–17 and 2017–18. Thus, CFLD data were analyzed from across minimum of 21 states (greengram) and maximum of 24 states (blackgram). The major variables analyzed were average yield obtained from the check plots and demonstrations plots. These yields were computed for yield advantages and also compared with the reported district level, state level, national level yields and the potential yields of the respective crops in the given states (data procured from secondary sources for the year 2017–18). Accordingly, the yield advantages (absolute as well as per cent) at various level were analyzed and their degree of variation was computed for all the crops across the seasons. The paper brings out the results of above analyses in objective manner.

OBJECTIVE:Task-based functional MRI (tb-fMRI) is now considered the standard, noninvasive technique in establishing language laterality in children for surgical planning. The evaluation can be limited due to several factors such as age, language barriers, and developmental and cognitive delays. Resting-state functional MRI (rs-fMRI) offers a potential path to establish language dominance without active task participation. The authors sought to compare the ability of rs-fMRI for language lateralization in the pediatric population with conventional tb-fMRI used as the gold standard.METHODS:The authors performed a retrospective evaluation of all pediatric patients at a dedicated quaternary pediatric hospital who underwent tb-fMRI and rs-fMRI from 2019 to 2021 as part of the surgical workup for patients with seizures and brain tumors. Task-based fMRI language laterality was based on a patient's adequate performance on one or more of the following: sentence completion, verb generation, antonym generation, or passive listening tasks. Resting-state fMRI data were postprocessed using statistical parametric mapping, FMRIB Software Library, and FreeSurfer as described in the literature. The laterality index (LI) was calculated from the independent component (IC) with the highest Jaccard Index (JI) for the language mask. Additionally, the authors visually inspected the activation maps for two ICs with the highest JIs. The rs-fMRI LI of IC1 and the authors' image-based subjective interpretation of language lateralization were compared with tb-fMRI, which was considered the gold standard for this study.RESULTS:A retrospective search yielded 33 patients with language fMRI data. Eight patients were excluded (5 with suboptimal tb-fMRI and 3 with suboptimal rs-fMRI data). Twenty-five patients (age range 7-19 years, male/female ratio 15:10) were included in the study. The language laterality concordance between tb-fMRI and rs-fMRI ranged from 68% to 80% for assessment based on LI of independent component analysis with highest JI and for subjective evaluation by visual inspection of activation maps, respectively.CONCLUSIONS:The concordance rates between tb-fMRI and rs-fMRI of 68% to 80% show the limitation of rs-fMRI in determining language dominance. Resting-state fMRI should not be used as the sole method for language lateralization in clinical practice.

In the last three decades, the use of herbal medications has been increasing for the treatment of various chronic disorders. Studies in the past have shown that many of these medicines could contain high levels of heavy metals, including lead. Therefore, we planned this study to evaluate the possibility of lead toxicity as the underlying cause in patients consuming these unnamed herbal medicines among patients presenting with significant abdominal pain. (Unexplained abdominal pain means pain in abdomen in which no etiology could be ascertained after all possible routine and specialized investigations including computerized axial tomography [CT] of the abdomen and upper gastrointestinal [UGI] endoscopy/colonoscopy). This is an observational case series of prospectively maintained data of all patients having unexplained abdominal pain and found to have an elevated blood lead level from 2011 to 2019. Lead toxicity was diagnosed when its blood lead level was >25 μg/dL. Total sixty-six patients with unexplained abdominal pain from 2011 to 2019 were recruited. Out of the sixty-six patients, seventeen had elevated blood lead levels. All seventeen patients had a history of ingestion of herbal medicines for more than 6 months. Among the seventeen patients, eight were taking it for infertility and sexual dysfunction, six for diabetes, two for arthritis and one for hypertension. Basophilic stippling was seen in one patient. Fourteen patients had low hemoglobin with a median value of 9.7 g/dL. Mean serum blood lead level was 87.1 μg/dL. None of them required anti-chelating agent. Lead toxicity owing to herbal medicine is not uncommon cause of unexplained abdominal pain. Most of these patients do not require a chelating agent for treatment. There is a need to bring these herbal medicines under strict regulations for displaying its constituents and their concentrations.

Replacement of natural aggregates (NAs) with recycled aggregates has been identified as a promising way of managing the waste associated with civil engineering. Therefore, the objectives of this study are also oriented towards managing the demolition waste in self compacting concrete (SCC). Efforts were made to estimate the impacts of replacing the NAs with the recycled-demolition waste aggregates (rDWAs) and addition of steel fibers (SFs) on the compressive strength (CS), split tensile strength (STS) and flexural strength (FS) of M30 grade fiber reinforced self-compacting concrete (FRSCC). A novel approach of central composite design (CCD) and response surface methodology (RSM) was adopted to design the experiments. The three levels of process parameters i.e., % replacement of NAs with rDWAs (X-1: %) and volumetric proportion of SFs (X-2: %) were 25, 50 and 75 and, 0.35, 0.70 and 1.05, respectively. The findings of this study revealed that at optimized conditions i.e., X-1 = 0.92 and X-2 = 0.89, the values of CS, STS and FS of FRSCC improved and were observed to be 35.67 MPa, 2.56 MPa and 4.96 MPa, respectively. From the findings of this study it was made evident that the replacement of NAs with rDWAs, in FRSCC-based structures, can prove efficient in the management of construction-associated waste. (C) 2021 Elsevier Ltd. All rights reserved.

We report a case of multifocal cystic meningioangiomatosis (MA), an exceptionally uncommon diagnosis even in patients with type 2 neurofibromatosis (NF2). A 2-year-old male with personal as well as family history of genetically-confirmed NF2 presented with incidental findings of MA after imaging for closed-head injury. Computed tomography and magnetic resonance imaging revealed multifocal subcortical and basal ganglia cysts, enhancing tumor-like vascular encasement, and a cerebellar ependymoma with atypical features. Given the paucity of available literature describing this pathology, imaging findings are discussed to further characterize this elusive disease. Radiologists must keep in mind that children with NF2 may not only present with MA, but also a constellation of MA with classic NF tumors, including ependymoma as in this case.

Malnutrition due to micronutrients and protein deficiency is recognized among the major global health issues. Genetic biofortification of wheat is a cost-effective and sustainable strategy to mitigate the global micronutrient and protein malnutrition. Genomic regions governing grain zinc concentration (GZnC), grain iron concentration (GFeC), grain protein content (GPC), test weight (TW), and thousand kernel weight (TKW) were investigated in a set of 184 diverse bread wheat genotypes through genome-wide association study (GWAS). The GWAS panel was genotyped using Breeders' 35 K Axiom Array and phenotyped in three different environments during 2019–2020. A total of 55 marker-trait associations (MTAs) were identified representing all three sub-genomes of wheat. The highest number of MTAs were identified for GPC (23), followed by TKW (15), TW (11), GFeC (4), and GZnC (2). Further, a stable SNP was identified for TKW, and also pleiotropic regions were identified for GPC and TKW. In silico analysis revealed important putative candidate genes underlying the identified genomic regions such as F-box-like domain superfamily, Zinc finger CCCH-type proteins, Serine-threonine/tyrosine-protein kinase, Histone deacetylase domain superfamily, and SANT/Myb domain superfamily proteins, etc. The identified novel MTAs will be validated to estimate their effects in different genetic backgrounds for subsequent use in marker-assisted selection.

It has become mandatory for all the domestic producers of urea to produce Neem Coated Urea (NCU) since 2015 to reduce overall input cost in farming and to ensure environmental sustainability through enhanced nitrogen use efficiency (NUE). The present study was carried out during 2017–19 with the aim to find out the awareness level of farmers about NCU and its impact on crop productivity. A total of 12,604 farmers across the country were interviewed by 458 Krishi Vigyan Kendras (KVKs). The results of the study showed that 76% farmers were aware about NCU and its effectiveness in increasing crop yield. Farmers of Delhi NCR, Haryana and Rajasthan were found to have the highest awareness while those of Arunachal Pradesh, Sikkim, and Jammu & Kashmir had the lowest level of awareness. Overall, 79% farmers were satisfied with its use. Use of N fertilizers decreased significantly in 2018 compared to 2017 in both the seasons (kharif and rabi), however, the existing rate of application was still significantly higher than recommended doses. The average yield enhancement due to use of NCU was 8.25%. The highest yield increase due to use of NCU was observed in Goa, Madhya Pradesh, and Andaman & Nicobar Islands. Results of the study implied that more emphasis is needed for dissemination of information about NCU through awareness, training and capacity building programmes for farmers and grassroots level extension functionaries.

Background: EMPOwER, a 12-week, double-blind (DB), randomized, placebo-controlled study evaluated the efficacy and safety of erenumab in adult patients with episodic migraine (EM) from Asia, the Middle East, and Latin America. This study analyzes the Indian experience for the use of erunumab for prevention of episodic migraine. Objective: The study aimed to evaluate the efficacy and tolerability of erenumab (70 mg and 140 mg) in EM patients from India. Methods: Randomized patients received monthly subcutaneous injections of placebo and erenumab 70 mg or 140 mg for 3 months. The primary endpoint was a change from the baseline in monthly migraine days (MMDs) at month 3. Other endpoints included achievement of ≥50%, ≥75%, and 100% reduction in MMD; a change in monthly acute migraine-specific medication treatment days; a change in patient-reported outcomes; and safety assessment. Results: Of the 539 patients screened, 351 patients were randomized (erenumab, 70 mg: n = 133 and 140 mg: n = 94; placebo: n = 124). The mean (±SD) age, disease duration, and MMD were 35.1 (±8.6) years, 6.77 (±6.01) years, and 7.82 (±2.89) days, respectively. The placebo-adjusted difference in mean MMD for erenumab 70 mg was -0.88 (95% CI, -2.16, 0.39; P = 0.174) days, and that for erenumab 140 mg was -1.01 (-2.42, 0.41; P = 0.164) days versus placebo. Secondary and exploratory endpoints demonstrated consistently better results in both erenumab dosage groups versus placebo. Treatment-emergent adverse events were comparable across groups (erenumab, 70 mg: 22.7% and 140 mg: 24.5%; placebo: 25.2%). Conclusion: Both doses of erenumab showed numerical improvement for efficacy endpoints and were well-tolerated in the Indian population. No new safety signals were reported.

The present study evaluates the Murraya Koenigii (CuLE) and Tinospora Crispa (TiSE) antimutagenic effect and the impact of industrial soil and solid waste leachate on Drosophila larvae. Larvae were exposed to leachate prepared at different pH (7, 4.93, 2.88) and treated with TiSE and CuLE at different concentration (4 g/L and 6 g/L) mixed with standard Drosophila medium. Emphasis was given to the binding interaction of heavy metals with proteins in Drosophila. The change in structure and molecular composition in Drosophila by leachate containing heavy metals induced toxicity has been studied by using Fourier transform infrared (FTIR) spectroscopy. Results from the study demonstrated that CuLE/TiSE administration restored the level of oxidative stress as evidenced by an enhanced antioxidant system and a decrease in lipid peroxidation and protein oxidation. The amide I and amide II bands spectral shifting revealed the binding interaction. The shift in the peak of PO2- asymmetric stretching might be due to compositional changes in nucleic acids. Single-cell electrophoresis was performed to detect the DNA damage which also proved to be ameliorated by administration of CuLE/TiSE. The result concludes that CuLE/TiSE may have great potential in the protection of Drosophila larvae from leachate induced oxidative stress through antioxidant and antimutagenic mechanisms this might help to cope with environmental toxicants.Communicated by Ramaswamy H. Sarma

Malnutrition due to micronutrients and protein deficiency is recognized among the major global health issues. Genetic biofortification of wheat varieties is both cost-effective and sustainable strategy to contain global micronutrient and protein malnutrition. Genomic regions governing grain zinc concentration (GZnC), grain iron concentration (GFeC), grain protein content (GPC), test weight (TW), and thousand kernel weight (TKW) were investigated in a set of 183 diverse bread wheat genotypes through genome wide association study (GWAS). The RIL population was genotyped using Breeders' 35K Axiom Array and phenotyped in three environments during 2019-2020. A total of 55 marker-trait associations (MTAs) were identified, of which four significant MTAs for GFeC on chromosome 2B, 3A, 3B, 6A and two for GZnC on chromosomes 1A and 7B. Further, a stable SNP was detected for TKW and also identified pleiotropic regions controlling GPC and TKW. In silico analysis revealed a few important putative candidate genes viz., F-box-like domain superfamily, Zinc finger CCCH-type proteins, Serine-threonine/tyrosine-protein kinase, Histone deacetylase domain superfamily and SANT/Myb domain superfamily proteins , etc. The identified novel MTAs will be validated to estimate their effects on different genetic backgrounds for subsequent use in marker-assisted selection (MAS).