In recent years, the attention of specialists has been attracted by a significant increase in allergic diseases, especially among children. Enzymatic hydrolysis with the participation of peptidases is considered the most effective measure to reduce the allergenicity of milk proteins. The study of the features of protein proteolysis by various enzymes, aimed at establishing optimal process parameters, is the subject of research in the production of hydrolysates with specified properties: a certain peptide and amino acid profile, reduced antigenic properties. At the same time, for a correct preclinical assessment of the safety and potential effectiveness of specialized food products, it is not enough only to determine the molecular weight distribution of peptide fractions in the composition. A necessary additional and informative indicator of reducing the potential allergenicity of the hydrolysate is the degree of preservation of the antigenic properties of the initial protein (residual antigenicity), determined by immunochemical methods. The preparation and subsequent comprehensive assessment of enzymatic hydrolysates of mare's milk proteins is of considerable interest, since, according to the results of clinical studies, it was suggested that upon allergy to cow's milk proteins mare's milk can be considered hypoallergenic. The purpose of this work was to obtain enzymatic hydrolysates of mare's milk proteins using domestic enzymes and their further physico-chemical and immunochemical characteristics. The object of the study was mare's milk protein obtained from skimmed mare's milk powder as a protein substrate. Two samples of enzymes from various manufacturers of the Russian Federation were used in the work. The results of the conducted studies show that the peptide profiles of hydrolysates vary greatly depending on the enzyme used, however, the multiplicity of antigenicity reduction practically does not depend on the nature of the enzyme, and does not depend on an increase in the enzyme/substrate ratio. The peptide profile and the multiplicity of reducing the antigenicity of hydrolysates of mare's milk proteins are influenced by ultrafiltration treatment of the obtained hydrolysates, which allows reducing the content of antigenic structures in the final product by several orders of magnitude.

Increasing the ability of the human body to adapt to physical stress is relevant from the standpoint of using foods for special uses containing functional food ingredients (FFI) with effectiveness proven in vivo. The purpose of this study was to evaluate the effect of FFI from Chenopodium quinoa grains with a high content of polyphenols and phytoecdysteroids on the physical endurance of male Wistar rats. Material and methods. The experiment was carried out during 36 days using 50 weaned male Wistar rats. The animals were randomly divided into 3 groups (n=12): Control, Run and Run-FFI. Rats of the Control and Run groups received a standard semisynthetic diet during the experiment. Rats of the Run-FFI group received a semi-synthetic diet with the addition of FFI in an amount of 0.055±0.003%, containing phytoecdysteroids (50.4±0.6 mg/g) and polyphenols (212.0±2.0 mg/g). During the experiment, the rats were assessed for their neuromotor function (grip strength of front paws), memory, and behavioral reactions in the "Elevated Plus Maze" (EPM), "Conditioned Passive Avoidance Reflex" (CPAR) and "Open Field" (OF) tests. Once a week, animals from the Run and Run-FFI groups were subjected to moderate physical load on a "Treadmill". On the 36th day of the experiment, the animals of these groups were subjected to exhausting physical load. Immediately after running, the animals were placed in metabolic cages to collect daily urine. At the end of the experiment, the content of corticosterone, the activity of catalase, indicators of protein, lipid and mineral metabolism, indexes of the liver functional state and antioxidant defense system parameters were analyzed in the blood serum; the level of prostaglandin E2 and dopamine were determined in daily urine. Results. Physiological tests (CRAR, OF) showed that weekly exercise increased anxiety in laboratory animals. The FFI introduction into the diet led to normalization of the assessed parameters (EPM). As a result of 36-day consumption of FFI against the background of physical loads, a significant decrease by 22% in the main stress marker, corticosterone, was revealed in the blood of rats, as well as significant increase by 23% in the stress inhibitor - prostaglandin E2 urinary excretion, compared with animals of the Run group to the level not differed from the indicators of the control animals. There were no differences in endurance performance between the Run and Run-FFI groups on the results of the exhaustive exercise. Consumption of FFI prevented the formation of excess ammonia, significantly reducing the level of urea in the blood and normalizing its excretion to control levels in the urine, which was increased in the Run group by 19%. Conclusion. The results obtained demonstrated the adaptogenic properties of the developed FFI in response to stress caused by weekly moderate and acute exhaustive physical activity. The obtained data on the biological effect of the developed FPI on the adaptive potential of laboratory animals will serve as an experimental basis for its inclusion in the composition of specialized foods.

We studied the effect of separate and combined influence of chronic forced physical activity reduction and high-fat and high-carbohydrate diet containing cholesterol on some indicators of carbohydrate, lipid, and cholesterol metabolism in growing male Wistar rats. Used combination of factors simulating a sedentary lifestyle and unhealthy diet did not have a synergistic effect on the selected biomarkers. On the contrary, the effect was antagonistic: body weight and appetite decreased and insulin resistance increased. The obtained results indicate certain prospects of hypercholesterolemia model using in preclinical studies of specialized food products to optimize the diet of individuals with disorders of carbohydrate and lipid metabolism.

Modern methods for extracting bioactive сompounds (BAC) from various raw materials are focused on efficacy and environmental awareness, involve the use of mathematical and statistical optimization methods, the choice of green solvents, and the use of additive extraction technology. The aim of this review was to present and briefly discuss up-to-date information on modern technological approaches to the production of plant BAС extracts for applying in food ingredients and foods for special dietary uses. Material and methods. For the main search of sources, the PubMed bibliographic database, Scopus and Web of Science databases, and the Google Scholar search engine were used. The search depth was 15 years. Results. The article presents a brief review of modern approaches to the extraction, concentration and purification of polyphenolic compounds from various plant materials. As an additive extraction technology aimed at destroying/increasing the permeability of the plant cell wall, a wide range of physical methods has been successfully used: ultrasound, microwave radiation, homogenization, application of a pulsed electric field, high hydrostatic pressure, cryo-crushing. A brief description of each method, its advantages and disadvantages are presented. Improving food safety and compliance with environmental regulations requires the choice of a safe, environmentally friendly and yet efficient extraction process. For these purposes enzymatic extraction, environmentally friendly solvents, such as deep eutectic solvents, supercritical fluid extraction and membrane technology are successfully used. The use of mathematical and statistical methods can reduce the total number of experimental trials and reduce the cost and time of experiments. The use of these methods together makes it possible to vary deliberately the process parameters in relation to a specific plant material and the final product, which provides the opportunity to isolate BAS concentrates from plant raw materials with a high yield. Conclusion. The introduction of innovative technological approaches for obtaining extracts of BAC of plant origin determines the prospects for the production of a wide range of foods for special dietary uses that meet high safety and efficiency requirements.

Amaranth (Amaranthus L.), like other pseudocereals as quinoa (Chenopodium quinoa Willd.), chia (Salvia hispanica L.) and buckwheat (Fagopyrum sp.), is a promising source of dietary protein. Depending on the subspecies and breeds of amaranth, the protein content in its grain is estimated from 13.1 to 21.5%, and its amino acid score varies over a significant range and can be limited. The aim of this study was to obtain a protein concentrate from amaranth (Amaranthus L.) grain of the Voronezh breed, enrich it with chicken egg protein, determine the amino acid score of the obtained protein module, and experimentally evaluate in vivo its true digestibility and biological value. Material and methods. The amaranth protein concentrate was obtained from grain according to the technological scheme, including its enzymatic treatment, alkaline extraction, acid precipitation of proteins, microfiltration and lyophilization. The amino acid composition and amino acid score of the concentrate were determined. The protein module was obtained by mixing amaranth protein concentrate and chicken egg protein in a weight ratio of 58:42. The true digestibility and biological value of the protein module has been determined in vivo. The experiment was carried out on 32 Wistar male rats divided into 2 groups (n=16 rats): control group 1 with a body weight of 118.7±3.1 g and experimental group 2 with a body weight of 119.5±3.0 g. Animals of groups 1 and 2 received diets in which egg protein and a protein module were used as a protein source, respectively. Within 15 days of the experiment, individual indicators of food intake and body weight gain of each animal were determined. From the 14th to the 15th day food intake was determined and feces were collected. The amount of nitrogen in the food and feces was determined for each rat using the Kjeldahl method. The true digestibility of the protein was determined according to obtained data. Results. The resulting amaranth protein concentrate contained 70.4±0.6% of protein, 17.0±1.0% fat, 9.8±0.8% carbohydrates, 1.8±0.2% ash, its moisture content was 1.4±0.1%. There were no significant differences in food intake and body weight gain between animals of both groups. The calculated value of the true digestibility of chicken egg protein was 98.8±0.1% for the control group 1, of the protein module was 99.0±0.1% for the experimental group 2, the differences between the groups were not significant. Conclusion. The results of amino acid analysis and the in vivo study of the true digestibility of the protein module (composition amaranth protein/chicken egg protein) indicate the absence of limitation relative to the amino acid scale of the "ideal" protein (FAO/WHO, 2007) and high true digestibility. The biological value of the protein module, calculated according to PDCAAS, is 99.0±0.1%, which confirms the prospects for its inclusion in specialized foods.

The choice of an optimal biological model of exogenous hypercholesterolemia is necessary for correct assessment of the cholesterol-lowering properties of bioactive substances and specialized food products and for validity of the experimental results. We studied the effects of cholesterol consumption in the composition of the standard semi-synthetic diet and high-fat high-carbohydrate diet for more than 3 months on lipid and cholesterol metabolism in male Wistar rats. Our findings suggest that cholesterol consumption in both diets led to a significant impairment of lipid and cholesterol metabolism, as well as an increase in insulin resistance in rats.

Due to the high prevalence of allergic diseases among the children, especially when transiting a child to bottle feeding at an early age, the development of dairy and other food products with reduced allergenicity is of high relevance and true importance nowadays. Meanwhile Russia still lacks sufficient production of raw materials and ingredients to create home-produced specialized food, including designated babies’ and children’s food. The use of whey milk proteins for the production of baby food has its advantage, as whey proteins feature pretty high digestibility and bioavailability: after eating the food based on whey proteins, the concentration of amino acids and peptides in the blood increases dramatically already within the first hour. However, it should be kept in mind that allergy to cow’s milk proteins is the most common symptom of food allergy, which is particularly often diagnosed in early postnatal age. Technological approaches to reduction of the potential allergenicity of the protein component shall take a leading place in the system of food control and food safety of children’s dairy products. The most efficient and widely used technological approach to reduce the potential allergenicity of food protein is the elimination of its antigenic structures, which approach makes it possible to include this protein into the composition of specialized food, which is intended for the people who suffer from food allergies or for the people exposed to the allergy risk. The allergenicity is reduced by means of enzymatic hydrolysis in combination with membrane processing of the obtained hydrolyzate. To obtain a peptide module with pre-determined physical and chemical parameters, an appropriate selection of initial protein, application of various enzymes, and ranging of technological parameters like pH, temperature, exposure time, enzyme-substrate ratio and other parameters is required.

The biomass of Arthrospira platensis cyanobacteria is a source of bioactive compounds such as chlorophylls, carotenoids, and, particularly, phycobiliproteins: C-phycocyanin and allophycocyanin. The wide range of biological activity shown by extracts with a high content of phycocyanins determines the prospects for their use as dietary supplements and ingredients of special foods. For food purposes, the degree of purity of phycocyanin concentrates, determined by the ratio of optical densities of their aqueous solutions at two wavelengths, namely D620/D280, must be greater than 0.7. Most methods for obtaining phycocyanin concentrates include laborious steps of fractional ammonium sulphate precipitation of protein from A. platensis biomass extracts followed by removal of salts solution. The use of membrane technology, specifically microfiltration, makes it possible to significantly intensify and simplify the process of obtaining phycocyanin concentrates. The aim of this research was to modify the method for obtaining a high-purity A. platensis phycocyanin concentrate by replacing the stage of ammonium sulfate precipitation of the protein by ultrafiltration of the extract followed by microfiltration. Material and methods. A sample of dry A. platensis biomass was used as a feedstock. Extraction of A. platensis biomass was carried out at a temperature of +40 °C for 3 h, the resulting suspension was centrifuged, and the supernatant was separated from the sediment. The obtained extract was subjected to ultrafiltration (membrane with a pore diameter of 30 kDa) followed by removal of the permeate containing low molecular weight impurities. The retentate was subjected to microfiltration (membrane with a pore size of 0.2 μm), concentrated by reverse osmosis and freeze-dried. Results. The content of C-phycocyanin and allophycocyanin in the dry concentrate was 42.0±1.3 and 7.0±0.3%, respectively, the degree of purity was 1.98. Conclusion. The scheme for obtaining A. platensis phycocyanin concentrate has been modified. A concentrate was obtained with a high degree of purity, allowing its use in food.

Amaranth is a widespread genus of predominantly annual herbaceous plants belonging to the Amaranthaceae family, which is one of the most widely used pseudocereals along with quinoa and buckwheat in nutrition. The aim of the research was to review and analyze the results of the studies on the characteristics of amaranth grain proteins, the effect of various food processing methods on their quality, and the prospects for using amaranth protein hydrolysates in therapeutic nutrition. Material and methods. For the main search for the literature, the PubMed bibliographic database was used, which covers about 75% of the world's medical publications. In addition, Scopus and Web of Science databases and non-commercial search engine Google Scholar were used. The depth of the search was 15 years. Results. The paper presents a brief review of modern approaches for obtaining amaranth protein isolates and concentrates, including the use of a complex of physicochemical methods: grinding, sifting, extraction at high pH values, defatting, ultrafiltration, centrifugation, isoelectric precipitation, and drying of the protein product. A comparative characteristic of amino acid content of protein fractions of pseudocereals is presented. Basically, leucine, isoleucine, and valine are limiting amino acids for the grain protein of various varieties of amaranth. When substantiating and developing modern effective food technologies for processing amaranth grain, the studies dedicated to the evaluation of their impact on the biological value of amaranth protein deserve special attention. Methods of grain fermentation, sprouting, steaming, malting, boiling can be used to increase the bioavailability and digestibility of its ingredients. The results of in vitro and in vivo studies indicate the presence of hypotensive, hypolipidemic and antioxidant activity of the amaranth protein and its hydrolysates what determines the prospects for their use as part of foods for special dietary uses and therapeutic nutrition. An analysis of the scientific publications presented in the review indicates an increase in demand for high-quality gluten-free products and an increase in the range of mass-consumption foods, such as bakery, pasta, flour confectionery, with pseudo-cereals in their composition, including amaranth. Conclusion. The high biological value and technological properties of amaranth protein concentrates/isolates determine the prospects for their use to create a wide range of specialized foods for various purposes.

The cyanobacterium Arthrospira platensis biomass is a promising food source of biologically active substances with pharmacological activity. The aim of this research was a brief review and analysis of experimental in vitro and in vivo studies of the antioxidant, hypoglycemic and hypolipidemic properties of A. platensis biomass, phycocyanins, and their chromophore - phycocyanobilin. Material and methods. For the main search of the literature, the PubMed Internet resource was used, the key component of which is the Medline article database, covering about 75% of the world's medical publications. In addition, Scopus and Web of Science databases were used. Search depth - 20 years. Search keywords: Arthrospira platensis, phycobiliprotein, C-phycocyanin, allophycocyanin, hypoglycemic effect, hypolipidemic effect, antioxidant activity, in vitro and in vivo studies. Results. A brief description of the composition of the cyanobacterium Arthrospira platensis biomass, methods of its cultivation, phycocyanins extraction methods is presented. The results of experimental studies indicate the presence of pronounced antioxidant properties of A. platensis biomass, mainly due to phycocyanins in its composition. The hypoglycemic and hypolipidemic effects of A. platensis biomass and extracted phycocyanins intake have been established in vivo when modeling carbohydrate and/or lipid metabolism disorders. The results of in vitro and in vivo studies indicate the presence of pronounced antioxidant properties of phycocyanins. Hypoglycemic effects are shown in particular in experiments on rats with hyperlipidemia and alloxan diabetes fed a diet enriched with A. platensis biomass and on KKAy mice, treated with C-phycocyanin extract. Conclusion. The analysis of the results of in vitro and in vivo studies of the antioxidant, hypoglycemic and hypolipidemic properties of A. platensis biomass and extracts with a high content of phycocyanins, presented in a brief review, suggests that their use in the diet of people with impaired carbohydrate and lipid metabolism is promising. Accordingly, from the standpoint of evidence-based medicine, clinical studies on the use of spirulina biomass and/or its extracts with a high content of phycocyanins as part of specialized foods intended for the prevention and/or dietary correction of carbohydrate and lipid metabolism disorders should be preceded by additional experimental physical-chemical, physiological and biochemical research.

Extracts from bilberry leaves and blueberries containing a wide range of biologically active compounds, including polyphenols, are of particular interest due to their antioxidant, hypoglycemic and hypolipidemic properties. In this regard, The aim of this research was to investigate in vivo the effect of a concentrate of blueberrie polyphenolic compounds with buckwheat flour on some physiological and biochemical parameters in C57Bl/6 mice with impaired carbohydrate and lipid metabolism induced by the consumption of a high fat high carbohydrate (HFHC) diet. Material and methods. The polyphenol concentrate was obtained by sorption of blueberry extract on grinded buckwheat flour. Total polyphenol content was determined by Folin-Ciocalteu method, profiles of anthocyanins, flavonoids and easily digested carbohydrates were determined by HPLC. An in vivo experiment was carried out using 84 male mice C57Bl/6 for 109 days. Animals were divided into 3 groups: control fed standard semisynthetic diet, control treated with HFHC diet and experimental group treated with HFHC diet with addition of blueberry polyphenol concentrate (60 mg-eq. of gallic acid/kg body weight). Food intake, body weight gain and fasting blood glucose levels were measured during the experiment. Grip strength of the front paws of the animals was measured weekly. Oral glucose tolerance and insulin resistance tests were carried out twice. Common physiological tests (Elevated Plus Maze and Passive Avoidance Test) were used to assess the anxiety and memory of animals. Glycated hemoglobin level was determined in blood, plasma was collected for leptin and insulin level determination. The hepatic levels of triglycerides and cholesterol were assessed. Results. The concentrate of polyphenols extracted from blueberries and sorbed on grinded buckwheat flour was obtained under conditions that made it possible to exclude the sorption of easily digested carbohydrates - glucose, fructose and sucrose on the flour. The total concentrate content of polyphenols was 65.5±0.7 mg-eq. gallic acid/g, anthocyanins - 27.3±2.7 mg/g, flavonoids - 1.2±0.1 mg/g. The consumption of the concentrate by C57Bl/6 male mice with carbohydrate and lipid metabolism disorders induced by a HFHC diet had a significant (p<0.05) hypoglycemic and hypolipidemic effect, reducing the area under the curve in the insulin resistance test by 5.7% and decreasing the insulin and leptin levels by 31.3 and 15.9%, respectively (relative to the animals of comparison group fed HFHC diet). The consumption of the concentrate had a significant (p<0.05) anxiolytic effect, reducing the anxiety of animals by 2.2 times, as determined in the Elevated Plus Maze test. Conclusion. The results of the study indicate the prospects for using the developed blueberry polyphenol concentrate adsorbed on buckwheat flour as part of specialized foods for the prevention of such alimentary dependent diseases as metabolic syndrome, obesity, and diabetes mellitus.

Biomass of Arthrospira platensis has a long history of consumption as a source of protein, a number of micronutrients and minor biologically active compounds. Specific organoleptic properties of Arthrospira platensis biomass (pronounced bitter taste) limit its use as a source of phycocyanins. The developed modern methods of phycocyanin extraction from A. platensis biomass make it possible to obtain concentrates with improved sensory characteristics destined for the inclusion in foods for special dietary uses. The aim of this brief review was to analyze the results of the studies on the assessment of the biological activity of phycocyanin extracted from the Arthrospira platensis biomass, substantiating the prospects of using their concentrates for inclusion in foods for various dietary purposes. Material and methods. The PubMed Web Database, including MEDLINE article database, covering about 75% of the world's medical publications, was used for the main search for the literature. In addition, Scopus and Web of Science databases were used. Search depth - 15 years. Search keywords: Arthrospira platensis, phycocyanins, safety, antioxidant activity, immunomodulatory properties. Results and discussion. C-phycocyanin and allophycocyanin are complexes of proteins with the pigment phycocyanobilin, their total content is about 50% of the content of all proteins in the A. platensis biomass. A significant number of toxicological studies indicate that there are no risks to human health when using phycocyanin-containing extracts of A. platensis. Evidence of the antioxidant effect of phycocyanins extracted from A. platensis biomass, their anti-inflammatory activity, immunomodulatory properties, was obtained experimentally in vitro and in vivo, as well as in clinical studies. Conclusion. Toxicological studies and experimental in vivo tests have shown the safe and effective use of Arthrospira platensis biomass extracts with a high content of phycocyanins as an additional means of dietary prevention and diet therapy. These data indicate the prospects for conducting additional studies on the possibility of including phycocyanin concentrates in specialized foods for various purposes.

The technology of innovative functional foodstuffs (FFSs) for the prophylaxis of type 2 diabetes involves supplementation with polyphenols (PPs) featuring hypoglycemic and/or hypolipidemic properties. The aim of the study presented was to develop an approach for the enrichment of food-grade protein matrix (PM)–coagulated egg albumen (CEA)–with PPs absorbed from cranberry juice (CJ). Concentration of total polyphenols (TPP) in gallic acid equivalents was determined using the Folin-Ciocalteu method. Concentration of total anthocyans (A) in cyanidin-3-glucoside equivalents was determined using pH-differential spectrophotometry; profile of individual anthocyans was determined according to State Standard GOST 32709-2014. Concentrations of simple saccharides (SS) were determined by the reverse-phase HPLC. The PPs from 100 ml portions of CJ with different juice concentration were sorbed by 10 g portions of CEA. After preliminary incubation and centrifugation the precipitates were lyophilized. The sorption rates of TPP and A were determined as a difference between the initial concentration in cranberry juice and in corresponding supernatants after centrifugation. The quantification of TPP and A in the lyophilized precipitates involved triple elution by 1% solution of HCl in 80% ethanol at 55° C and subsequent ultrasound treatment. Maximal specific sorption for TPP and A from 100% CJ was 14.0 mg-eq. of gallic acid/g and 1.48 mg/g, respectively. Sorption rate for SS on the CEA was 30.3±3.1% of their initial concentration in CJ. The TPP/SS ratio in the enriched PM was 2.0 times higher as compared to CJ. The results of the study can be used in the development of new FFSs with antidiabetic properties.

For correct and reliable experimental in vivo assessment of antistress effect of various bioactive substances, appropriate biomodels reproducing stress and organism response to stress in laboratory animals should be chosen. We chose treadmill test for simulating exhaustive physical load and forced immobilization accompanied by disorders of physiological and psychological condition. Verification of the models used indicates their wide applicability for testing certain biological manifestations under reproduced stress exposure.

Objective: Quinoa grains (Chenopodium quinoa Willd.) contain a large number of secondary metabolites. Phytoecdysteroids and polyphenols, secondary metabolites, are of great interest for research today. The aim of the study is a method development for the extraction of biologically active substances (BAS) from quinoa grains to obtain a functional food ingredient (FFI) enriched with phytoecdysteroids and polyphenols. Materials and methods: The content of phytoecdysteroids at each stage of quinoa extract production was determined by two methods: HPLC with a spectrophotometric detector and HPLC with a mass spectrometer. The content of total polyphenols was determined by the Folin-Ciocalteu method. The flavonoid profile was determined using a liquid chromatography system with a diode array spectrophotometric detector and a triple quadrupole mass spectrometric detector. Results: The chosen technological scheme for the production of FFI can concentrate more than 200 times the major phytoecdysteroid 20-hydroxyecdysone major in quinoa in the final product relative to the raw material. The maximum yield of flavonoids in the final product was observed in cases of 20 and 40% ethanol extraction. Conclusion: These results lead to the conclusion that highly enriched sources of BAS can be obtained using fairly simple technological approaches and commercially available reagents from quinoa grains.

The overview presents the literature data and the results of our own research on prospects of using the chicken eggs as the basis of functional foods. The composition of chicken eggs and their components, characteristics of egg white proteins properties are presented thereto. The biologically active compounds included into egg composition are analyzed. The data on the biological value of egg white are given. The characteristic of egg white foaming ability is presented. It has been shown that the ability of proteins to form stable intermolecular structures, especially with partially denaturated proteins, allows them forming viscoelastic superficial films that ensure foam stability. The high foaming ability of chicken egg protein macromolecules is directly related to their interphase properties, i. e. the ability to form interphase layers at the “liquid — gas” interface. The foaming properties of the various egg proteins are not equal, and therefore they contribute to foaming properties at various extents. The model of egg white proteins gelation is considered and the factors influencing the gelation process are described. It has been shown that very important changes in proteins properties are caused by denaturation. The proteins lose their ability to hydrate; the protective aqueous shell around the globules disappears, the proteins stick together, grow larger and lose solubility. This process is called coagulation. The influence of denaturation and aggregation on variations of protein properties is described below. Data on protein fortification with functional ingredients (calcium, iodine, plant polyphenols) and creation of functional egg and meat foods are presented here.

This work aimed to develop the technology for the production of functional food ingredients (FFI) with hypoglycemic and hypocholesterolemic action. These are organic forms of zinc and chromium and complex of bilberry polyphenols sorbed on brown buckwheat flour. Soy protein isolate hydrolysate was obtained using pancreatin as an enzymatic agent. The hydrolysis was conducted at 50-52°C for 3 hours. The product was then pasteurized at 75°C for 20 min. Organic forms of zinc and chromium were obtained by mixing the hydrolysate with zinc chloride and chromium chloride water solutions followed by micro- and nanofiltration. The bilberry fruits polyphenols were sorbed on the buckwheat flour by mixing them. The mixture was then centrifuged, the precipitate was lyophilized. Organic sources of zinc and chromium were included in the composition of specialized food product (SFP) for patients with metabolic syndrome (MS) as FFI. The introduction of the product into the hypocaloric diet of MS patients led to improvements in lipid metabolism. In essence, lowered total cholesterol decreased low-density lipoproteins and triglycerides levels, lowered atherogenic coefficient. During in vivo study hypoglycemic properties of FFI (bilberry polyphenols complex with buckwheat flour) were established. Experimental mice had significantly lower glucose, glycated haemoglobin and relative liver weight on the background of FFI consumption. Firstly, the obtained data proves the possibility to extract, concentrate and save beneficial properties of polyphenols through their sorption on the biopolymer plant matrices. Secondly, the obtained data proves the prospects of the use of essential microelements organic forms for preventive diet therapy.

The effects of vanadium complex with enzymatic hydrolysate of soy protein (V-EHSPI) were studied in male Wistar rats with induced disorders of carbohydrate and lipid metabolism. The content of vanadium (IV) in the studied complex was 15.8 mg/g dry product. High-lipid high-carbohydrate diet was used to induce disorders of lipid and carbohydrate metabolism. Addition of vanadium in three different doses to the diet over 100-day experiment reduced body weight gain and the levels of glucose, insulin, leptin, and triglycerides. V-EHSPI produced beneficial effects on carbohydrate and lipid metabolism even in a dose 5 μg/kg body weight/day (calculated from the mean food consumption over the entire treatment period). Significant inhibition of growth and changes in the weight of organs in animals treated with V-EHSPI attested to toxicity of vanadium in the studied dose range.