Selective 137Cs+ sequestration is challenging due to its high solubility, environmental mobility, and the influence of excessive competitive ions. Herein, we demonstrate an effective strategy for Cs+ separation by constructing zeolite-type metal sulfide as "cesium ion sieve" (CIS), obtaining GaGeS-1 with "ion-sieving effect". Its structure features a zeolite-type metal sulfide framework with sodalite (SOD) topology based on supertetrahedral T2 clusters. GaGeS-1 with radiation resistance possesses a maximum Cs+ adsorption capacity of 332.52 mg/g and ultrafast adsorption kinetics with removal rate (RCs) of 97.06% within 1.5 min. It achieves highly selective Cs+ capture under excessive competing ions, even for actual industrial 137Cs⁺-liquid-waste (RCs > 90%). Single crystal structure analysis and density functional theory calculation reveal that "ion-sieving" originates from strong Cs+···S2- interaction, flexible-robust framework, and optimal Cs+ coordination in SOD cage. Furthermore, GaGeS-1 is integrated with gelatin to fabricate GaGeS-1/Gel nanofibrous electrospun membranes for a multi-stage filtration system, enabling efficient, continuous, and recyclable treatment of mixed Na/Cs wastewater (3.58 L/m2, RCs = 99.39%) and Cs+-contaminated river water (2.63 L/m2, RCs = 99.23%). This study pioneers an efficient "CIS" by developing zeolite-type metal sulfide, and provides a universal assembly method toward practical application, opening new insight into radiocesium remediation and advanced nuclear waste management.

Protein homeostasis plays a pivotal role in disease pathogenesis, making targeted protein modulation a transformative approach for therapeutic development. Current protein-targeting strategies primarily converge on two complementary approaches: selective degradation of pathogenic proteins and direct modulation of aberrant protein function. Recent advances have enabled the implementation of both strategies in nanoscale architectures, exemplified by nanoscale proteolysis-targeting chimeras (nano-PROTACs) for controlled protein degradation and nanoscale artificial protein modulators (nano-APROMs) for precise functional regulation. By integrating molecular recognition with nanoscale engineering, these systems offer enhanced delivery efficiency, spatiotemporal controllability, and expanded regulatory modalities. However, fundamental questions remain regarding their structural design principles, structure-activity relationships, and the mechanisms governing dynamic nano-bio interactions. This review highlights advances in the rational design of nano-PROTACs for improved delivery and fine-tuning of ubiquitin-proteasome degradation kinetics, and atomic-level engineering strategies for nano-APROMs that enable systematic reprogramming of aberrant protein function. First, we analyze the nanoscale architectural design principles of nano-PROTACs, focusing on stimulus-responsive allosteric architectures. Subsequently, we assess therapeutic implementation approaches employing nano-PROTACs, examining both standalone treatment modalities and synergistic combination regimens. Third, we discuss the design strategies and therapeutic applications of nano-APROMs for precise and controllable protein modulation, with a focus on atomic-scale nanomaterials-based approaches, including nanocarrier-mediated targeted delivery and atomic-level engineering for protein fine-tuning. Finally, we provide a forward-looking perspective on key challenges and untapped opportunities at the forefront of protein-targeting nanomedicine, proposing actionable directions for future research and clinical development.

Developing rapid, integrated sensing platforms for on-site pesticide monitoring is critical for food safety evaluation. However, current field-deployable sensors often suffer from poor stability and matrix interference. Herein, a catalytic/fluorescent multifunctional nanostructure (Ru-CN/UCNPs@ZIF-8) was engineered by co-encapsulating oxidase-like Ru-CN and upconversion nanoprobes within a ZIF-8 framework. This integrated architecture enables the highly sensitive detection of chlorpyrifos via the specific inhibition of an acetylcholinesterase-mediated fluorescence recovery cascade. To realize on-site visualization, the sensing system was incorporated into a GelMA hydrogel. Coupled with smartphone imaging, this hydrogel platform achieved a quantitative limit of detection of 1 ng mL-1, exceptional 28-day storage stability, and reliable recoveries (88.78%-112.38%) in fruit and vegetable matrix extraction. Finally, to ensure stable signal acquisition under complex field conditions, a custom portable device featuring an independent excitation module and power supply was fabricated. By effectively isolating environmental light, this hardware-assisted strategy provides a highly stable and precise tool for rapid food safety screening.

Alkaline phosphatase (ALP), a hydrolase commonly found in living organisms, shows significantly elevated levels in serum of patients suffering from liver metastasis. Rapid and efficient detection of ALP activity in serum is thus critical for assessing cancer prognosis. In this study, we developed a composite nanoprobe (UCNPs@ZIF@PDA) based on upconversion nanoparticles (UCNPs), specifically NaErF4:Tm (0.5 mol%)@NaLuF4, characterized by strongly correlated energy levels, for the sensitive and stable detection of ALP in complex environments. To improve stability, UCNPs were encapsulated with zeolitic imidazolate framework-8 (ZIF-8). The upconversion luminescence (UCL) of the UCNPs was subsequently quenched by polydopamine (PDA), which was formed via in-situ polymerization of dopamine on the ZIF-8 surface. ALP catalyzed the conversion of L-ascorbic acid trisodium 2-phosphate (AAP) to ascorbic acid, which in turn triggered the degradation of PDA and restored the UCL. Through this mechanism, the limits of detection for ALP in aqueous solution and serum are 0.19 and 0.42 U/L, respectively. Moreover, the probe demonstrates precise imaging of ALP in cellular environments and successfully distinguishes ALP levels in samples with and without liver metastasis. These findings highlight the strong potential of UCNP-based composite fluorescent probes as a novel and efficient tool for ALP detection in clinical applications.

The increasing prevalence of microplastics in aquatic environments poses serious threats to ecosystems and human health, creating an urgent need for rapid, sensitive, and reliable detection methods. Herein, we report a flower-like δ-MnO2/Ag nanocomposites as a high-performance surface-enhanced Raman scattering (SERS) substrate for microplastic detection. The hybrid material was fabricated via a simple two-step approach, in which layered δ-MnO2 nanosheets served as both structural supports and reducing agents for the in situ growth of Ag nanoparticles (NPs). The resulting hierarchical architecture generated abundant electromagnetic hot spots, while strong synergistic interactions between δ-MnO2 and Ag NPs significantly enhanced SERS performance. Using p-aminothiophenol (PATP) as a probe molecule, the optimized δ-MnO2/Ag-3 composite achieved an enhancement factor of 3.44×107 with excellent reproducibility (RSD = 4.76%). For polystyrene (PS) microplastics, it exhibited a linear detection range of 5–300 ppm with an ultralow detection limit of 1.17 ppm. Recoveries of 92.5–112.8% for PS and coexisting pollutant bisphenol A (BPA) in tap water samples confirmed its applicability in complex environmental samples. The enhanced SERS activity originated from the combined effects of localized surface plasmon resonance and interfacial charge transfer. This study presents a robust semiconductor-metal hybrid SERS platform for sensitive microplastic detection and environmental monitoring.

Chronic refractory wounds in diabetic patients pose a substantial clinical challenge owing to fluctuating pH levels, bacterial infections, and compromised healing mechanisms. Here, we have developed a metabolic dynamic self-adaptive cobalt-based hydrogel dressing that incorporates spatially confined biomineralized enzyme (glucose oxidase) nanoflowers with high catalytic efficiency and a polyvinylpyrrolidone (PVP) hydrogel matrix with superior unidirectional water absorption capacity. The elevated glucose metabolism in diabetic wounds was harnessed to enable efficient cascade catalysis to convert glucose into hydrogen peroxide (H2O2), followed by Co2+/Co3+ redox reactions that sustain spatiotemporal reactive oxygen species (ROS) generation. The low redox potential of the cobalt hydrogel allows robust catalytic activity (>75%) across a wide pH range (4.0-9.0) for over 50 h to adapt to the entire process of wound repair. Moreover, the hydrogel reduces the diffusion distance between ROS and bacteria, resulting in potent broad-spectrum antibacterial activity, including biofilm eradication, against multidrug-resistant Escherichia coli, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus (MRSA). In an immunosuppressed mouse model of diabetic trauma, it was demonstrated that under hyperglycemic conditions, the GCP hydrogel facilitated a wound closure rate of 97.4% by day 12. Our strategy paves the way for the development of advanced intelligent wound dressings featuring dynamic, self-adaptive microenvironments and persistent antimicrobial activity.

Spatiotemporal control of RNA therapeutics remains a fundamental challenge limiting clinical translation. Here, we develop a photoactivatable CRISPR/Cas13d (paCas13d) system that enables non-invasive, light-controlled RNA manipulation in deep tissues. Through structure-guided engineering, we identify optimal split sites within RfxCas13d and create light-switchable fragments using CRY2PHR/CIBN optogenetic dimerization. To overcome the limited tissue penetration of blue light, we engineer polyethylenimine-functionalized upconversion nanoparticles (UCNPs-PEI) that serve dual roles as gene carriers and photon transducers, converting tissue-penetrating near-infrared (NIR) to blue light. The UCNPs-PEI@paCas13d system achieves precise spatiotemporal control of RNA targeting within bone tissue in vivo. In a murine steroid-associated osteonecrosis model, NIR-activated paCas13d achieves robust TET3 knockdown, disrupting the TET3-5hmC-PTEN axis that drives glucocorticoid-induced osteocyte apoptosis. This targeted intervention prevents bone deterioration, with treated mice showing preserved trabecular architecture, enhanced bone volume, and favorable shifts in bone turnover markers, while maintaining systemic glucocorticoid efficacy. Our platform combines the programmability of CRISPR/Cas13d with non-invasive optical control, offering a versatile approach for treating diseases requiring localized RNA modulation while minimizing systemic effects.

Percutaneous locoregional drug injection based on hydrogel therapy under image guidance is performed to limit the progression of hepatocellular carcinoma (HCC) and extend the waiting time for liver transplantation patients. However, achieving uniform distribution and sustained retention of drugs within the tumor bed remains a critical bottleneck urgently requiring breakthroughs in the current field of oncology. Here, an acid-labile thermosensitive hydrogel (denoted as NCD) with efficient magnetothermal functionality was developed by incorporating iron oxide nanoparticles (CION) and DOX into the ortho-ester-functionalized thermosensitive polymer matrix (poly(N-isopropylacrylamide270)-ortho ester-poly(ethylene glycol)). Engineered for multimodal therapy, the NCD hydrogel utilized an acid-cleavable backbone to achieve sustained DOX release (77.4 ± 2.1% at pH 6.5, 72 h), markedly exceeding the control release of 26.8 ± 2.7% and yielding uniform tumor drug distribution. Its thermosensitive PNIPAM matrix (LCST ≈ 32 °C) enabled injectable sol-gel transition at body temperature, allowing easy administration (maximum injection pressure: merely 6.0 ± 0.3 N) and forming a depot for prolonged drug retention. Combined with CION-enhanced magnetothermal therapy, ultrasound-guided NCD delivery suppressed orthotopic liver tumor growth, positioning it as a promising bridging strategy to transplantation.

The selective capture of 137Cs+ from complex radioactive liquid waste presents a persistent and critical challenge due to its high solubility and strong mobility, particularly under harsh alkaline environments. Herein, two supertetrahedral cluster-based microporous metal chalcogenides (STC-MMCs) featuring two-fold interpenetrating frameworks, namely (C2H8NO)6In10S18·(C2H7NO)·5H2O (1) and (C2H8N)4.5(C3H10N)1.5In10Se18·4H2O (2) were synthesized. They exhibit high adsorption capacity (qmCs = 286.68 mg/g for 1, qmCs = 276.85 mg/g for 2), rapid kinetics (equilibrium time tCs ≤ 5 min) and excellent selectivity and reusability for Cs+ capture in neutral environments. Supertetrahedral clusters as nodes and interpenetrating open frameworks confer their structural stability, providing the prerequisite for Cs+ capture in alkaline environments. They demonstrate high distribution coefficients (KdCs > 104 mL/g) and removal rates (RCs > 80%) even under pH of 12. Unprecedently, they retain excellent selectivity (KdCs > 103 mL/g) in the alkaline solutions (pH = 11 and 12) despite the presence of competing Na+, K+, Mg2+ and Ca2+. Notably, they maintain efficient Cs+ uptake in actual alkaline salt lake brine. Specifically, the single-crystal to single-crystal structural transformation for 2 reveals the basic mechanism at the molecular level. The excellent Cs+ capture is attributed to ion exchange between Cs+ and cations within the channels, the high flexibility of the interpenetrating open framework with highly negative charge, and the strong interaction of Lewis soft basic sites Se2- for Cs+. This work pioneers the systematic research on metal chalcogenides for radiocesium remediation in alkaline environments and provides new insights for treating alkaline radioactive liquid waste.

Real-time visual monitoring of food freshness is crucial for ensuring food safety and reducing waste. Herein, fluorescent carbon dots (H-CDs and D-CDs) were synthesized via hydrothermal and solvothermal methods, respectively, using citric acid and urea as precursors. The fluorescence intensity of H-CDs increased gradually with pH, accompanied by a visible color change from green to yellow, whereas D-CDs exhibited weaker pH sensitivity. DFT calculations revealed a smaller energy band gap for H-CDs (2.88 eV) than for D-CDs (2.73 eV), indicating a more favorable electronic structure for pH-responsive behavior. A pH-responsive label (H-CDs/CS) was fabricated by incorporating H-CDs into a carboxymethyl cellulose/sodium alginate matrix. In contrast to the negligible response of the D-CDs/CS label, the H-CDs/CS label exhibited superior sensitivity to ammonia, with a 17.18% decrease in ΔE value and a 70.80% increase in fluorescence intensity. Remarkably, when used to monitor shrimp freshness, the H-CDs/CS label effectively distinguished freshness levels across a wide temperature range (25 ℃ to -23 ℃), with its colorimetric and fluorescent signals showing a strong correlation with pH (R2 = 0.9954 for ΔE and R2 = 0.9901 for fluorescence). This work establishes a portable real-time visual assessment platform, offering a promising strategy for intelligent food packaging.

Sorption-based atmospheric water harvesting (SAWH) is a promising strategy to alleviate global water scarcity. However, the practical application of SAWH is hindered by the low water production efficiency of sorbent, especially in arid climate. This limitation results from sluggish sorption-desorption kinetics, a consequence of the long transport path required for vapor and liquid water due to sorbent stacking. Herein, we report a thin hygroscopic polyampholyte hydrogel layer on a solid porous substrate, which greatly mitigate stacking. The development dramatically improves the sorption-desorption kinetics, which allows 61 sorption-desorption cycles per day in an arid climate and results in 33.3 L kg -1 theoretical freshwater collection within 24 h (30% RH). Besides, the strategy demonstrates remarkable scalability—even at a hundred-gram scale, the hydrogel layer can stably produce 1.16 L of water per day (10.5 L kg⁻¹ day⁻¹) in the dry, cold winters of central China (12.4 ℃, 70.4% RH). Our work provides a promising solution to mitigate the severe water crisis in arid climate.

In this paper, a Cu-MOF/TiO2 composite (CT-1) sonosensitizer with excellent production of reactive oxygen species (ROS) such as O-1(2) and center dot OH and higher performance in inhibiting tumor cell growth through SDT was successfully prepared and characterized. Meanwhile, using ROS probes and U251 cells as a tumor cell model, the sonodynamic mechanism and sonodynamic antitumor activity in vitro of CT-1 were studied. The results suggested that Cu-MOF/TiO2 heterojunction was successfully constructed. The combination of Cu-MOF and TiO2 significantly enhanced the absorption of visible light by the composite sonosensitizer, making better use of the energy of sonoluminesence, and the Z-scheme heterojunction formed enhanced carrier separation, thereby generating more ROS such as O-1(2) and center dot OH, which greatly improved the sonodynamic activity of the sonosensitizer. The in vitro results of sonodynamic inhibition of U251 tumor cell growth showed that the Cu-MOF/TiO2 composite CT-1 had higher sonodynamic antitumor activity than both Cu-MOF and TiO2, and the sonodynamic inhibition of U251 tumor cell growth by CT-1 was dose-dependent. When the concentration of CT-1 was 50 mu g/mL, the viability of U251 cells was only 27.20%, demonstrating an excellent sonodynamic antitumor effect. The above research results confirmed that the formation of heterojunction was an effective means to enhance the sonodynamic activity of the sonosensitizer and would provide a research basis for the development of new efficient sonosensitizers and the in-depth study and application of SDT.

Multifunctional coatings combining electromagnetic protection, fire safety, and environmental durability are attractive for flexible protective textiles. Here, an interface-regulated coating was fabricated by embedding Fe3O4@TiO2@polyaniline (FTP) core-shell fillers in an ivy-inspired poly(vinyl phosphonic acid-co-hydroxyethyl acrylate-co-styrene) (PVHS) matrix and depositing it on polyester fabric. The Fe3O4 core provides magnetic loss, the TiO2 interlayer promotes interfacial polarization and ultraviolet attenuation, and the polyaniline shell contributes dielectric loss and conductive pathways. Meanwhile, phosphonic acid groups in PVHS act as proton donors to regulate polyaniline doping, thereby strengthening polarization loss and improving impedance matching at low filler loading. The optimized FTP@PVHS-4 achieved a minimum reflection loss (RLmin) of −37.25 dB and an effective absorption bandwidth (EAB) of 6.27 GHz, while the coated fabric retained strong absorption with −32.74 dB and 6.73 GHz, respectively. PVHS also promoted condensed-phase charring, reducing the peak heat release rate by 91.2% relative to pristine polyester. In addition, the fabric exhibited excellent ultraviolet shielding, with a protection factor of 1993.21 and average ultraviolet‑A transmittance of 0.05%, while maintaining coating integrity and textile flexibility. This study demonstrates that interfacial electronic regulation and biomimetic polymer design provide an effective strategy for lightweight, durable, and multifunctional electromagnetic protective fabrics.

Colloidal gels (CGs) are attractive carriers for functional nanoparticles (NPs) in biomedical applications. However, the current interfacial design of CG networks often lacks efficient disassembly mechanisms, resulting in limited intelligent responsiveness, constraining their advancements in precision medicine. Herein, we developed an interfacial mineralization strategy to fabricate a mineralized magnetic colloidal gel (MMG) tailored for disassembling in the acidic tumor microenvironment. MMG comprises electrostatically attracted mineralized magnetic core-shell Fe3O4@calcium phosphate (CaP) NPs and gelatin NPs, exhibiting outstanding injectability and magnetic-heating effect, and presenting potential for minimally invasive interventional therapy of tumors. Benefiting from the dissolution of the interfacial CaP layer in an acidic microenvironment, the storage modulus of MMG decreased from 1400 to 400 Pa after 48 h, while the drug-release efficiency increased from ∼35% to ∼70%. In comparison, the unmineralized magnetic CG showed few changes in mechanical properties and exhibited a low drug-release efficiency of ∼20%. The acid-triggered disassembly of MMG's network confirmed the feasibility of precision chemotherapy. Additionally, MMG-mediated magnetic hyperthermia and chemotherapy significantly improved a synergistic therapeutic effect in tumor-bearing mice and ultrasound-guided interventional hepatic tumor rabbits. These findings demonstrate that the interfacial mineralization strategy provides an innovative approach to imparting CG's network with microenvironment-responsive controllable disassembly behavior.

3D design has effectively enhanced photothermal materials water evaporation performance via utilizing side surfaces. However, for photothermal/photocatalytic composite evaporators which can effectively degrade volatile organic compounds (VOCs) to prevent their contamination in condensate, introducing 3D design should promote VOCs escaping from the evaporator sides surface, reducing efficiency of VOCs photocatalytic degradation. Herein, a bioinspired unidirectional silk fibroin (SF)-Ag2S/Ag3PO4 3D evaporator is reported to achieve synergy between enhancing water evaporation and maintaining VOCs photocatalytic degradation efficiency. The unidirectional channels are modified with Ag2S/Ag3PO4 composite as both photothermal and photocatalytic agents. By promoting directional water transfer, the bioinspired unidirectional scaffold achieves an evaporation rate of 2.86 kg m-2 h-1 under 1 sun with the assistance of side-surface evaporation. Furthermore, the unidirectional channels effectively restrict the horizontal migration and the escape of VOCs from side surface, directing their transport toward the top surface, where the VOCs can be photocatalytically degraded by Ag2S/Ag3PO4 composite. Accordingly, bioinspired SF-Ag2S/Ag3PO4 scaffold maintains 91.9% VOCs removal performance under 1 sun in 10 mg L-1 phenol-contaminated water. Moreover, compared with the evaporator without directional VOCs transportation, bioinspired scaffold shows 96% and 41% increase in VOCs removal and water evaporation, respectively, under 2 suns in 100 mg L-1 phenol solution.

Disintegrating hydrogels offer the advantages of synergistically enhancing therapeutic efficacy in locoregional percutaneous treatment for early hepatocellular carcinoma, enabling on-demand drug delivery with reduced side effects. However, current disintegration processes suffer from limited precise control and weak tissue penetration. Herein, we constructed an injectable ferrimagnetic hydrogen bonding cross-linked hydrogel (named as FPH) as a locoregional percutaneous agent by integrating ferrimagnetic nanoparticles into a polyvinyl alcohol (PVA) crosslinked network. This hydrogel enables remote magnetothermally triggered disintegration under an alternating magnetic field (AMF), with its disintegration temperature precisely tunable by adjusting the hydrogel's solid content. Under magnetic heating effect, FPH achieved remote "gel to disintegration" behavior at a desired temperature range of around 47 °C, effectively suppressing tumor cells and minimizing harm to normal tissues. By contrast, other commonly used hydrogen-bonded network of gelatin hydrogel disintegrated below body temperature or agarose hydrogel disintegrated over 70 °C. Benefiting from magnetothermal-controlled disintegration, drug-loaded FPH exhibited an increased release efficiency from ∼8 % (without AMF) to ∼45 % (with AMF) within 1 h. Following ultrasound-guided percutaneous delivery, FPHDOX exhibited synergistic efficacy with magnetic hyperthermia and disintegration-mediated chemotherapy in rabbit liver tumors. Additionally, FPH is fabricated using clinically approved pharmaceutical excipients, ensuring excellent biocompatibility. This strategy inspires the design of spatiotemporally controllable disintegrating hydrogels with limitless tissue penetration depth, and expands their potential in percutaneous hepatocellular carcinoma treatment.

Achieving rapid and scar-free wound repair is a key goal in the field of regenerative medicine. Herein, a dynamically Schiff base-crosslinked hydrogel (F/R gel) with phase-adaptive regulating functions is constructed to integratedly promote rapid re-epithelization with suppressed scars on chronic infected wounds. Specifically, the gel effectively eliminates multidrug-resistant bacterial biofilm at infection stage via antimicrobial activity of ε-polylysine firstly dissociated from hydrogel matrix in infectious microenvironment, and interrupts the severe oxidative stress-inflammation cycle at wound site by the released ceria nanozyme, thus stimulating a pro-regenerative environment to ensure tissue repair. Subsequently, fibroblast growth factor/c-Jun siRNA co-loaded microcapsules gradually disintegrate to release drugs, facilitating neoangiogenesis and cell proliferation but simultaneously blocking c-Jun overexpression for fibrotic scar suppression. Notably, the F/R gel facilitates normal-like skin regeneration with no perceptible scars formed on infected male mouse wound and female rabbit ear wound models. Our work offers a promising regenerative strategy emphasizing immunomodulatory and fibroblast subtype modulation for scarless wound repair.

Antibacterial hydrogels are promising for combating infections and promoting wound healing. Nevertheless, excessive antibiotics induce resistance, and high metal ion levels cause cytotoxicity, complicating healing. Here, we introduce a hydrogel incorporating polydopamine-coated bioactive glass (BGs@PDA) on reduced graphene oxide (rGO) with photothermal therapy (PTT) and silver nanoclusters (AgNCs) for synergistic antibacterial treatment. This design enables rapid bacterial eradication and controlled release. Near-infrared-assisted heating provides noninvasive, targeted hyperthermia, killing bacteria quickly. Post-PTT addition of low-dose AgNCs reduces toxicity while enhancing antimicrobial efficacy and biocompatibility. BGs@PDA-loaded rGO prevents sedimentation, improves photothermal conversion and conductivity, and stabilizes the hydrogel structure. Constructed from chitosan and hydroxyethyl cellulose, the hydrogel is cross-linked by PDA and rGO, enhancing mechanical strength, adhesion, self-healing, free radical scavenging, and continuous wound exudate absorption. PDA encapsulation facilitates BGs degradation, improving the wound microenvironment. In vivo studies confirm accelerated healing and potent synergistic antibacterial effects, indicating its potential as a low-dose, antibiotic-free alternative for clinical wound infection management.

The effectiveness of orally delivered probiotics in treating gastrointestinal diseases is restricted by inadequate gut retention. In this study, we present a magnetically controlled strategy for probiotic delivery, which enables controlled accumulation and residence of probiotics in the intestine. The magnetically controlled probiotic is established by attaching amino-modified iron oxide (Fe3O4-NH3+ NPs) to polydopamine-coated Lacticaseibacillus rhamnosus GG (LGG@P) through electrostatic self-assembly and named as LGG@P@Fe3O4. In a simulated gastrointestinal environment, LGG@P@Fe3O4 maintains both structural stability and probiotic viability. Furthermore, the LGG@P@Fe3O4 clusters can be easily manipulated by an external magnetic field, inducing directional movement and aggregation. In vitro simulations demonstrated significant accumulation and retention of LGG@P@Fe3O4 under a magnetic field, with the optical density (OD) value of the suspension decreasing from ∼1.17 to ∼0.29. In contrast, the OD value of the suspension without a magnetic field remained at its original level (∼1.15). In a mouse model with intragastrically administered LGG@P@Fe3O4, the group exposed to a magnet exhibited stronger gut fluorescence after 24 h. The magnetically controlled probiotic delivery strategy offers an easy manufacturing and feasible method to enhance the effectiveness of probiotics in treating gastrointestinal diseases.