The role of hilar lymph node metastasis (LNM) in intrahepatic cholangiocarcinoma (ICC) was investigated and a simple postoperative risk-stratification system combining LNM and preoperative anemia was established to predict the outcome of patients with ICC after curative resection. A retrospective analysis was conducted on 384 patients who underwent hepatectomy for ICC. Both postoperative clinical outcomes and long-term oncological outcomes were systematically evaluated. Subsequently, predictive models were developed to assess the risks of tumor recurrence, overall survival (OS) and recurrence-free survival (RFS). The primary cohort demonstrated 65.2

Colorectal cancer (CRC) is a common malignant tumor in the digestive tract. Circular RNAs (circRNAs) have been identified as crucial regulators of tumorigenesis. However, the role and potential mechanism of circ_0004585 in CRC are poorly understood. The expression of circ_0004585, microRNA-338-3p (miR-338-3p), and zinc finger protein X-linked (ZFX) was detected by quantitative real-time PCR and Western blot. Cell proliferation, cell cycle arrest, apoptosis, and angiogenesis were evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), 5-Ethynyl-2′-deoxyuridine (EdU), flow cytometry and tube formation assays. Western blot assay was applied to detect the expression of epithelial-mesenchymal transition (EMT)-related proteins and MEK/ERK signaling pathway-related proteins. A xenograft model was used to analyze tumor growth in vivo. The targeted relationship between miR-338-3p and circ_0004585/ZFX was verified by a dual-luciferase reporter assay. Circ_0004585 and ZFX were up-regulated, while miR-338-3p was down-regulated in CRC tissues and cells. Silencing of circ_0004585 inhibited proliferation, angiogenesis, and EMT and triggered apoptosis in CRC cells. Consistently, circ_0004585 depletion blocked tumor growth in vivo. Circ_0004585 contributed to CRC cell development via sequestering miR-338-3p. Also, miR-338-3p hindered the malignant progression of CRC cells by targeting ZFX. Circ_0004585 activated MEK/ERK pathway via regulating ZFX. Circ_0004585 facilitated CRC progression through modulating miR-338-3p/ZFX/MEK/ERK pathway, which might provide a potential therapeutic target for CRC.

Background. Circular RNAs (circRNAs) possess key functions in the pathogenesis of hepatocellular carcinoma (HCC). Nonetheless, the actions of individual circRNAs in HCC remain undefined.Methods. circ_0031242, miR-944, and MAD2L1 expression were quantified by qRT-PCR. Transwell assay was utilized to examine cell invasion and migration. Glucose consumption and lactate production were measured to assess the impact on glycolysis. The relationships among circ_0031242, MAD2L1, and miR-944 were examined via luciferase reporter assay.Results. circ_0031242 was notably augmented in HCC. Loss of function of circ_0031242 hindered cell proliferation, invasion, migration, glycolysis, and promoted apoptosis, as well as impeding HCC tumor growth. circ_0031242 directly targeted miR-944. Inhibition of miR-944 counteracted the effects of si-circ_0031242 on HCC cells. Additionally, miR-944 was proved to directly target MAD2L1 in HCC cells. Moreover, the promotion of MAD2L1 was able to rescue the inhibition of high miR-944 expression on HCC cell progression. Meanwhile, circ_0031242 involved the post-transcriptional modulation of MAD2L1 through miR-944.Conclusion. This study suggested that circ_0031242 regulated tumor cell progression and tumor growth through the miR-944/MAD2L1 axis in HCC.

目的 研究槲皮素抗结肠癌细胞SW480生长的作用,并探索其可能的作用机制.方法 通过CCK-8法来检测SW480细胞在槲皮素处理下的增殖变化;运用HE染色、电镜观察和流式细胞术方法检测SW480细胞在槲皮素处理下的凋亡变化;运用Transwell检测槲皮素处理情况下SW480细胞在体外的粘附、运动和侵袭能力变化;运用明胶酶谱分析法检测槲皮素处理情况下SW480细胞基质金属蛋白酶分泌能力的变化.结果 CCK-8法检测显示槲皮素处理使SW480细胞受到了显著的增殖抑制,且具有剂量和时间依赖性;HE染色、流式细胞术及电镜分析发现槲皮素明显诱导细胞凋亡,并将SW480细胞周围阻滞于G2/M期;此外,槲皮素也能够使结肠癌SW480细胞的体外侵袭和运动能力受到抑制,且该抑制作用呈剂量依赖性关系;明胶酶谱法显示槲皮素处理后,SW480细胞对金属蛋白酶2(matrix metalloproteinase-2,MMP-2)和金属蛋白酶9(matrix metalloproteinase-9,MMP-9)的分泌均下降.结论 槲皮素对人结肠癌SW480细胞的增殖有明显的抑制作用,并能促进SW480细胞发生凋亡.槲皮素对结肠癌SW480细胞的迁移及侵袭能力均表现出抑制作用,并且呈剂量依赖性,这可能和槲皮素能够抑制MMP-2及MMP-9的分泌有关.

目的:探讨槲皮素(Que)调控5-氟尿嘧啶(5-FU)诱导的结直肠癌SW480细胞耐药及自噬的作用及机制.方法:采用浓度递增诱导法建立5-FU耐药细胞株SW480/5-FU,MTT法设置Que高、中、低剂量组(10、20、40μmol/L)和对照组.MTT法检测各组细胞的5-FU耐药性,TUNEL染色法检测细胞凋亡,免疫荧光检测细胞自噬活性,Western blot检测细胞p糖蛋白(Pgp)、多药耐药相关蛋白1(MRP1)、三磷酸腺苷结合转运蛋白G超家族成员2抗体(ABCG2)、LC3Ⅰ、LC3Ⅱ、p62、丝/苏氨酸激酶(AKT)、p-AKT、雷帕霉素靶蛋白(mTOR)和p-mTOR蛋白表达.结果:5-FU抑制SW480/5-FU细胞的半抑制浓度(IC50)明显高于SW480细胞(P<0.05),耐药指数(RI)=13.74.与对照组相比,Que中、高剂量组5-FU抑制SW480/5-FU细胞的IC50明显下降(P<0.05),耐药逆转倍数分别为2.27和4.03.与对照组相比,Que中、高剂量组细胞抑制率、凋亡率和自噬活性明显升高(均P<0.05),蛋白的表达均明显升高(均P<0.05),P-gp、MRP1、ABCG2、p62、p-AKT/AKT和p-mTOR/mTOR蛋白表达明显下降(均P<0.05).结论:Que可以以剂量依赖性的方式逆转SW480/5-FU细胞的5-FU耐药性,诱导细胞自噬,其作用机制可能与抑制AKT/mTOR的磷酸化有关.

目的:探讨经皮肝穿刺胆道镜取石术(PTCSL)治疗复杂肝胆管结石的临床疗效及复发率.方法:纳入2015年10月至2017年10月收治的96例复杂肝胆管结石患者,按手术方式分为PTCSL组(n=49)与开腹组(n=47),术后两组患者均随访1～3年.对比分析两组术后结石取净率、优良率、围手术期指标、疼痛程度、免疫反应、肝功能恢复情况及结石复发率.结果:PTCSL组结石取净率、优良率优于开腹组(P<0.05);手术时间、术中出血量、恢复肛门排气时间、住院时间少于开腹组(P<0.05),切口长度短于开腹组(P<0.05),术后疼痛程度及结石复发率低于开腹组(P<0.05),且PTCSL组患者免疫力高于开腹组,肝功能恢复较好(P<0.05).结论:PTCSL可有效清除结石,术后康复快,复发率及并发症发生率低,是治疗复杂肝胆管结石的有效术式.

<正>肝癌是世界范围内最常见的10种恶性肿瘤之一,我国大陆和台湾都是肝癌的高发地区,发病率和病死率有上升趋势,在多数地区各类肿瘤中位列前3位。近些年肝癌的治疗技术有很大进展,治疗效果显著提高,而大肝癌(肿瘤直径≥5cm)

目的总结原发性大肝癌射频消融后的治疗结局和经验。方法 2006年1月至2011年6月,我院共对64例失去手术机会的原发性大肝癌患者实施了超声引导下的经皮射频消融。肿瘤最大直径5～8.4cm,平均直径(5.7±0.62)cm。并对其临床资料、随访资料进行总结分析。结果射频消融后肿瘤完全坏死率为70%,随访1～62个月,中位生存期为15个月,1、3、5年生存率分别为59.5%、32.3%、6.3%。结论对于不可切除的原发性大肝癌,射频消融是较有效的局部治疗方法。

Objective To observe the inhibitory effect on the growth and proliferation of human colon carcinoma SW480 cell line in vitro induced by quercetin. Methods SW480 cells were incubated in vitro by cell biology technique. By means of CCK-8, the effect of quercetin on SW480 cell was studied, the morphological change of SW480 cell was investigated by light microscope and electron microscope. Flow cytometry using propidium iodide (PI) staining was used to determine the cell cycle and cell apoptotic rate. Results Quercetin inhibited the growth of SW480 cell by a time- and dose- dependent manner (P < 0.05). The amount of SW480 cell treated by quercetin decreased. Apoptotic corpuscles were found by electron microscope. The apoptosis rate of SW480 cell was improved, and induced cell cycle arrest in G2/M in does-dependent manner of quercetin (P < 0.05). Conclusion Quercetin can inhibit the growth of SW480 cell by a time- and dose- dependent manner and induce the apoptosis of SW480 cell in vitro.

Objective To study the surgical therapeutic measures of large primary liver cancer.Methods From January,2002 to January,2011,116 patients with large primary liver cancer received surgical therapy in our department.The clinical data and follow-up data were summarized.Results The one-year,3and 5-year survival rates of liver resection were 79%,61%,38%.Conclusion The principle treatment for large primary liver cancer is resection.

Objective:To observe the effect on the invasion of human colon cancer SW480 cell line in vitro induced by quercetin.Methods: Human colon cancer cells SW480 were treated with different doses of quercetin,and the invasion of SW480 was determined by Transwell chamber,the expressions of MMP-2 and MMP-9 were detected by gelatin zymography.Results: Transmembrane cell and the expressions of MMP-2 and MMP-9 of quercetin group decreased in dose-dependent manners(P<0.05).Conclusion: Quercetin can inhibit invasion of human colon cancer cells,and decreasing the expression of MMP-2 and MMP-9 may play an important role in its mechanism.

Objective To investigate the appearance and structure changes of SW 480 after treated by quercetin,and to explore the effects of Quercetin on invasion,metastasis and adhesion in human SW480 cell.Methods The morphological changers of SW480 were investigated by microscopically and electro microscopically examination.The effect of different concentrations of Quercetin on SW480 cell for 24,48,72 hour was observed;Matrigel Basement Membrane Matrix was used to investigate the effects of Quercetin on invasion,metastasis and adhesion in human SW480 cell.Results Light microscopic observation showed that the amount of SW480 cells decreased,partial cells volume shrunk,the morphology of SW480 was irregular,cytoplasmic condensation,vacant bubbles,condensed nuclei and condensed chromatin after treated with Quercetin.Under electron microscope,SW480 cell exhibited that endoplasmic reticulum swollen,ribosome decreased,cytoplasmic condensation,pyknotic nuclei and apoptotic bodies;The amount of human colon carcinoma cell line SW480 treated by Quercetin decreased with dose and time dependent.After treated by Quercetin,the ability of invasion,metastasis and adhesion descended with dose and time dependent.Conclusion Quercetin can inhibit the growth of human colon carcinoma cell line SW480 by inhibiting the ability of invasion,metastasis and adhesion and induce cell apoptosis.Many key points in the invasive and metastasis process were enrolled in.

Objective:To explore the effects of apigenin on invasion,metastasis and adhesion in human SW480 cell.Methods: Matrigel basement membrane matrix was used to investigate the effects of apigenin on invasion,metastasis and adhesion in human SW480 cell.Results: After treated by apigenin,the ability of invasion,metastasis and adhesion descended with dose and time dependent.Conclusion: Apigenin could inhibit the ability of invasion,metastasis and adhesion.It could inhibit many key points in the invasive and metastasis process.

Objective To observe the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin and to detect the expression of Cathepsin-D in SW480 cell treated by quercetin.Methods Zymogram analysis assay was used to analyze the effect on the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480.The expression of Cathepsin-D in SW480 cell treated by quercetin was measured by immunohistochemistry method.Results Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased.With increasing concentration of quercetin,the secretion of MMP-2 and MMP-9 decreased.Immunohistochemistry method demonstrated the positive expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time-and dose-dependent manner.Conclusions Quercetin can suppress the secretion of matrix metalloproteinases and the expression of Cathepsin-D in human colon carcinoma cell line SW480.These are probably the reason that quercetin inhibits invasion and metastasis of colon cancer cells.

<正>2008年4月29日卫生部发布第三次全国死因调查结果表明,城乡居民恶性肿瘤病死率属于世界较高水平,并呈持续增长趋势。防治肿瘤刻不容缓,而广泛存在于多种水果和蔬菜中的黄酮类化合物槲皮素与肿瘤发生发展关

Background/Aim: The extent of gastric resection and lymphadenectomy for curative treatment of distal gastric cancer remains controversial. The present study investigated the efficacy of total re;section of the lesser curvature as radical surgery for distal gastric carcinoma.Methodology: Patients with pathologically confirmed advanced distal gastric cancer seen at our hospital from 2003 to 2006 were randomly selected to receive either total resection of lesser curvature (Group A, N=60) or traditional subtotal gastrectomy (Group B, N=60), both with D2 lymph node dissection. Patient and tumor characteristics, lymph node metastases, and surgical outcomes were analyzed.Results: Three-year survival rates were 56.7% and 28.3% for Groups A and B, respectively (p=0.042). A total of 467 (30.5%) and 225 (24.7%) tumor-positive lymph nodes were resected in Groups A and B, respectively (p=0.002). Tumor recurrence rate was 1.6% (1/60) in Group A and 11.6% (7/60) in Group B (p=0.061).Conclusion: Total resection of the lesser curvature with D2 level lymph node dissection prolonged patient survival and decreased tumor recurrence. This surgical approach should be considered for the treatment of patients with distal gastric carcinoma.