Objective To explore the therapeutic effect of Tongsaimai Tablets combined with clopidogrel bisulfate in treatment of acute cerebral infarction.Methods Patients(108 cases)with acute cerebral infarction from January 2020 to March 2023 were randomly divided into control(54 cases)and treatment(54 cases)group.Patients in the control group were po administered with Clopidogrel Bisulfate Tablets,75 mg/time,once daily.Patients in the treatment group were po administered with Tongsaimai Tablets on the basis of the control group,5 tablets/time,three times daily.Patients in two groups were treated for 14 d.After treatment,the clinical evaluation was evaluated,and the symptom relief times,the scores of FMA and NIHSS,the levels of IL-6 and Hcy,and adverse reaction in two groups before and after treatment were compared.Results After treatment,the clinical effective rate of patients in the treatment group(98.15%)was significantly higher than that in the control group(83.33%,P<0.05).After treatment,the time of symptom improvement in the treatment group was significantly earlier than that in the control group(P<0.05).After treatment,the NIHSS score in two groups was significantly decreased,while the FMA score was significantly increased(P<0.05),and these scores in the treatment group were significantly better than those in the control group(P<0.05).After treatment,the levels of serum inflammatory factors IL-6 and Hcy in the treatment group were significantly lower than those in the control group(P<0.05),which in the treatment group was significantly lower than that in the control group(P<0.05).After treatment,the incidence of adverse reactions in the treatment group(5.56%)was significantly lower than that in the control group(12.96%,P<0.05).Conclusion The curative effect of clopidogrel bisulfate combined with Tongsaimai Tablets in treatment of acute cerebral infarction is definite,which can effectively improve the clinical symptoms,correct the state of cerebral nerve injury,weaken the inflammatory response and enhance the motor function of patients.
Objective To investigate the clinical efficacy of Rotigotine Patches combined with amantadine in treatment of Parkinson's disease.Methods From November 2019 to December 2021,124 patients with Parkinson's disease admitted to the outpatient and inpatient departments of Bayannur Hospital were selected,and all patients were divided into control group and treatment group by random number table method,with 62 patients in each group.Patients in the control group were po administered with Amantadine Hydrochloride Tablets,0.1 g/time,twice daily.Patients in the treatment group were given Rotigotine Patches on the basis of the control group,applied the product to the skin at the same time every day and keep it for 24 h,the initial dosage was 2 mg/24 h,once daily,and applied to another part of the skin when changing the patch each time,to avoid repeated use within 14 d of the same part.The weekly dosage was increase by 2 mg/24 h until the effective dosage(not more than 8 mg/24 h).The treatment course of both groups was 12 weeks.The clinical efficacy of the two groups was observed.The scores of the unified Parkinson's Disease Rating Scale(UPDRS),the Psychosocial Adaptation Scale(PAS),the Parkinson's Sleep Scale-2(PDSS-2),the eight item Parkinson's Disease Questionnaire(PDQ-8),the Parkinson's Disease Non motor Symptom Scale(PD-NMSS),and the levels of serum superoxide dismutase(SOD),acetylcholine(Ach),and interleukin-6(IL-6)were compared between the two groups before and after treatment.Results After treatment,the effective rate of the treatment group was 93.55%,which was significantly higher than that of the control group(80.65%)(P<0.05).After treatment,the scores of Ⅰ,Ⅱ,Ⅲ,and Ⅳ in UPDRS and the total scores of scales in both groups were significantly decreased compared with those before treatment(P<0.05).The decrease was more significant in the treatment group(P<0.05).After treatment,PAS scores in both groups were significantly increased,while PDSS-2,PDQ-8 and PD-NMSS scores were significantly decreased(P<0.05).The improvement was more significant in the treatment group(P<0.05).After treatment,the serum SOD level in both groups was significantly increased,while the serum Ach and IL-6 levels were significantly decreased(P<0.05).After treatment,the serum SOD level in the treatment group was significantly higher than that in the control group,and the serum Ach and IL-6 levels were significantly lower than that in the control group(P<0.05).Conclusion Rotigotine Patches combined with amantadine can achieve a definite effect in treatment of Parkinson's disease,and can effectively improve the clinical symptoms,sleep status and quality of life of patients,and can further enhance the antioxidant capacity of the body,reduce inflammatory damage and inhibit the hyperactivity of Ach,which is conducive to delaying the progress of the disease with good safety.
Objective To explore the clinical efficacy of Jisheng Shenqi Pills and losartan potassium in treatment of chronic glomerulonephritis.Methods Patients(88 cases)with chronic glomerulonephritis in Suzhou Hospital of Anhui Medical University from January 2019 to February 2023 were randomly divided into control(44 cases)and treatment(44 cases)group.Patients in the control group were po administered with Losartan Potassium Tablets,100 mg/time,once daily.Patients in the treatment group were po administered with Jisheng Shenqi Pills on the basis of the control group,1 pill/time,three times daily.Patients in two groups were treated for 2 months.After treatment,the clinical evaluation was evaluated,and the levels of renal function indicators CysC,NGAL and RBP,the levels of inflammatory indicators IL-6,IFN-γ and TNF-α in two groups before and after treatment were compared.Results After treatment,the clinical effective rate of the treatment group was 90.91%,which was significantly higher than that of the control group(75.00%,P<0.05).After treatment,the levels of serum CysC,urinary NGAL,urinary RBP,and serum IL-6,IFN-γ and TNF-α levels in two groups were significantly decreased(P<0.05),and the decrease in the treatment group was significantly higher than that in the control group(P<0.05).Conclusion The combination of Jisheng Shenqi Pills and Losartan Potassium Tablets has good clinical efficacy in the treatment of chronic glomerulonephritis,which can effectively improve the renal function and inflammatory response.
Artificial intelligence algorithms include machine learning algorithms and deep learning algorithms,which can be applied to discover drug targets,discover and optimize lead compounds,determine candidate drugs,and optimize druggability.Artificial intelligence algorithms can achieve model building and high-throughput virtual computing through complex big data system learning.When applied in drug research and development,it can shorten the research and development cycle to a certain extent,reduce input costs,and thereby improve the success rate of research and development.In this article,the research progress of machine learning algorithms and deep learning algorithms applied in drug development was reviewed,in order to provide reference for the further development of the combination of artificial intelligence technology and drug development.
Objective To mine the signals of adverse drug events(ADE)for selinexor based on the FAERS database,and to provide a reference for safe use of drugs in clinical.Methods The reported odds ratio(ROR)and proportional reported ratio(PRR)methods were used to mine selinexor adverse event reports between the second quarter of 2019 and the third quarter of 2023,and the mined signals were classified and statistically analyzed using the Medical Dictionary of Regulatory Activities(MedDRA)preferred system organ classification(SOC)and preferred termy(PT).Results A total of 4 991 adverse event reports involving 19 868 PTs with selinexor as the first suspected drug were retrieved.After computational screening,118 selinexor ADE signals were obtained,involving 16 SOCs.Selinexor ADE signals were basically consistent with the known relevant safety information,and the adverse events were mainly focused on gastrointestinal system diseases,systemic diseases and various reactions at the drug administration site,various types of examinations,metabolic and nutritional diseases.In addition,53 ADE signals not included in the drug specification were detected,such as constipation,fall,drowsiness,and abnormal renal function.Conclusion FAERS-based big data can provide a comprehensive and in-depth analysis of post-marketing adverse reactions of drugs,suggesting that the clinic should pay close attention to the gastrointestinal toxicity,hematological toxicity and risks not mentioned in the instruction manual of selinexor at the early stage of treatment,and take preventive measures so as to ensure the safety of clinical use.
Extended and controlled release micropill capsules is common sustained-release preparations,and have gradually become one of the hotspots in the research and development of sustained-release preparations.Whether the research on the formulation process of extended and controlled release micropill is sufficient is not only a basic prerequisite for ensuring drug quality,but also a foundation for meeting clinical needs.Comprehensive control of raw materials and auxiliary materials,rational prescription design,and stable and reproducible production processes are the focus of prescription process research.Based on the guiding principles of The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use(ICH),relevant domestic and foreign literature,as well as actual cases encountered in reviews,and combined with the characteristics of extended and controlled release micropill capsules,this article focuses on the research focus of extended and controlled release micropill capsules formulation technology,and proposes general considerations in the formulation process development,in order to provide ideas and suggestions for the research and registration application of extended and controlled release micropill capsules.
Objective To explore the clinical effect of Liuwei Dihuang Pills combined with empagliflozin in treatment of type 2 diabetes.Methods A total of 102 patients with type 2 diabetes admitted to Beijing Friendship Hospital(Pinggu Hospital),Affiliated to Capital Medical University from March 2020 to February 2023 were selected as the study objects.All patients were divided into control group and treatment group according to different treatment methods,with 51 cases in each group.Patients in the control group were po administered with Empagliflozin Tablets,10 mg/time,once daily.Patients in the treatment group were po administered with Liuwei Dihuang Pills on the basis of the control group,1 pill/time,twice daily.The treatment course of both groups was 12 weeks.After treatment,the clinical efficacy was evaluated,and the blood glucose indexes,islet function related indexes,quality of life scale(DMQLS)score,neutrophil lymphocyte ratio(NLR),serum triglyceride(TG),total cholesterol(TC),apoA1,and feeding inhibitory factor-1(Nesfatin-1)were compared between the two groups before and after treatment.Results After treatment,the total effective rate of the treatment group was 96.08%,which was significantly higher than that of the control group(82.35%)(P<0.05).After treatment,fasting blood glucose(FPG),2 h postprandial blood glucose(2 h PG)and glycosylated hemoglobin(HbA1c)in 2 groups were significantly decreased compared with before treatment(P<0.05).After treatment,FPG,2 h PG and HbA1c in the treatment group were lower than those in the control group(P<0.05).After treatment,HOMA-IR and DMQLS scores in both groups were significantly decreased compared with before treatment,while QUICKI scores were significantly increased compared with before treatment(P<0.05).After treatment,HOMA-IR and DMQLS scores in the treatment group were lower than those in the control group,and QUICKI scores were higher than those in the control group,with statistical significance(P<0.05).After treatment,whole blood NLR,serum TG,TC and Nesfatin-1 levels in 2 groups were significantly decreased compared with before treatment,and serum ApoA1 levels were significantly increased(P<0.05).After treatment,whole blood NLR,serum TG,TC and Nesfatin-1 levels in the treatment group were lower than those in the control group,and serum ApoA1 level was higher than that in the control group(P<0.05).Conclusion Liuwei Dihuang Pills combined with empagliflozin has achieve satisfactory effect in treatment of type 2 diabetes,and can effectively reduce the inflammatory state of the body,correct the disorder of lipid metabolism,which can improve the blood sugar control,islet function and quality of life of patients.
Objective To explore the key active ingredients and action targets and pathways of Citrus Reticulata in treatment of metabolic associated fatty liver disease through the effects of regulating qi by network pharmacology and molecular docking techniques,in order to investigate its possible pharmacological basis and potential molecular mechanisms.Methods The main chemical components and related targets of action of Citrus Reticulata were collected through the systematic pharmacology database and analytical platform of traditional Chinese medicine.The disease related targets related to regulate qi and metabolic associated fatty liver disease were retrieved from the GeneCards database,and the regulate qi targets,metabolic associated fatty liver disease targets and pharmacodynamic targets were mapped to derive the efficacy targets of the regulate qi effect of Citrus Reticulata for the treatment of metabolic associated fatty liver disease.The efficacy targets were entered into the STRING database to derive a PPI interaction network and visualized using Cytoscape 3.9.0 software.The GO and KEGG pathways were enriched by the DAVID database.After screening the key active compounds and target genes of Citrus Reticulata,molecular docking was carried out to predict the binding of the active compounds to the core targets,to identify the possible molecular mechanisms of action and to construct a"compound-target-pathway"network map.Results A total of 63 active ingredients and 540 corresponding targets were identified in Citrus Reticulata,580 in regulating qi and 478 in metabolic associated fatty liver disease.56 Key targets and 16 important signaling pathways were identified,such as PI3K/Akt signaling pathway,Ras signaling pathway,insulin signaling pathway,AMPK signaling pathway,NF-κB signaling pathway,etc.The main components of Citrus Reticulata's regulating qi efficacy in treating metabolic associated fatty liver disease are nobiletin,naringenin,and hesperidin C,and the key targets are Akt1,PPARα,PPARγ,and EGFR.In addition,molecular docking showed that nobiletin,naringenin and hesperidin bound well to Akt1,PPARγ,JUN,ESR1,and PPARα,respectively.Conclusion Naringenin,nobiletin,and naringenin in Citrus Reticulata may be the main active components for its rational qi effect,which can regulate the biological effect processes of oxidative stress,inflammatory response and lipid metabolism in treatment of metabolic associated fatty liver disease in a multi-target and multi-pathway manner,providing a theoretical basis and scientific basis for the in-depth study of Citrus Reticulata in treatment of metabolic associated fatty liver disease in clinical practice.
Objective To investigate the impacts of dexmedetomidine on the proliferation,apoptosis,and Wnt/β-catenin pathway of endometrial cancer cells.Methods Ishikawa and RL95-2 cells were divided into control group,dexmedetomidine 1,10,100 nmol/L,and dexmedetomidine(100 nmol/L)+ LiCl group.CCK-8 method and EdU were applied to detect cell proliferation,flow cytometry was applied to detect cell apoptosis,Western blotting was applied to detect the expression levels of PCNA,Bcl-2,Bax,β-catenin,c-Myc,and cyclin D1.An endometrial cancer nude mice model was constructed and divided into control group,dexmedetomidine group,and DEX + LiCl group,the mass and volume of the tumor are measured,HE staining was applied to observe the morphology of transplanted tumor tissue,immunohistochemical methods were applied to detect the expression of Ki-67 and β-catenin proteins in transplanted tumor tissue.Results Compared with control group,the A value,EdU positive cell rate,migration cell number,invasion cell number,PCNA,Bcl-2,β-catenin,c-Myc,and Cyclin D1 protein expression of Ishikawa and RL95-2 cells were obviously reduced after treatment with different concentrations of dexmedetomidine,the apoptosis rate and Bax expression increased(P<0.05).Compared with dexmedetomidine 100 nmol/L group,the A value,EdU positive cell rate,migration cell number,invasion cell number,PCNA,Bcl-2,β-catenin,c-Myc,and Cyclin D1 protein expression in dexmedetomidine + LiCl group obviously increased,the apoptosis rate and Bax expression decreased(P<0.05).Dexmedetomidine was able to inhibit the mass and volume of transplanted tumors,promote tumor apoptosis,and reduce the expression of β-catenin and Ki-67 proteins(P<0.05),LiCl reversed the inhibitory effect of dexmedetomidine on transplanted tumors(P<0.05).Conclusion Dexmedetomidine can inhibit proliferation and promote apoptosis of endometrial cancer cells,and its mechanism of action may be related to the inhibition of the Wnt/β-catenin signaling pathway.
Objective To explore the clinical effect of Zaizao Shengxue Capsules combined with ferrous succinate in treatment of iron deficiency anemia of deficiency of both qi and blood.Methods Patients(120 cases)with iron deficiency anemia of deficiency of both qi and blood from January 2019 to March 2023 were randomly divided into control(60 cases)and treatment(60 cases)group.Patients in the control group were po administered with Ferrous Succinate Tablets,0.1 g/time,three times daily.Patients in the treatment group were po administered with Zaizao Shengxue Capsules on the basis of the control group,5 grains/time,three times daily.Patients in two groups were treated for 3 months.After treatment,the clinical evaluation was evaluated,SI,SF and HGB levels,TCM syndrome scores in two groups before and after treatment were compared.Results After treatment,the clinical effective rate of the treatment group was significantly higher than that of the control group(98.33%vs 88.33%,P<0.05).After treatment,the levels of SI,SF and HGB in two groups were significantly higher than those before treatment(P<0.05),and which in the treatment group were significantly higher than those in the control group(P<0.05).After treatment,the TCM syndrome score in two groups was significantly lower than that before treatment(P<0.05),and which in the treatment group was significantly lower than that of the control group(P<0.05).Conclusion Zaizao Shengxue Capsules combined with ferrous succinate can effectively improve the levels of ferritin and hemoglobin in patients with iron deficiency anemia of deficiency of both qi and blood,and improve the TCM syndrome.
Objective To explore the clinical effect of Ganhai Weikang Capsules combined with esomeprazole sodium in treatment of reflux esophagitis.Methods A total of 122 patients with reflux esophagitis treated in Anyang People's Hospital from March 2021 to December 2022 were selected and divided into control group and treatment group according to random number table method,with 61 cases in each group.Patients in control group were iv administered with Esomeprazole Sodium for injection,40 mg of this product was dissolved in 100 mL 0.9%sodium chloride injection,20—30 min/time,twice daily.Patients in the treatment group were po administered with Ganhai Weikang Capsules on the basis of the control group,6 grains/time,three times daily.The treatment course of both groups was 7 d.The clinical efficacy of two groups was observed.The changes of typical symptom scores,Hamilton Anxiety Scale(HAMA),17 Hamilton Depression Scale(HAMD-17)scores,GerdQ scores,endoscopic scores,and esophageal 24-hour pH impedance monitoring parameters before and after treatment were compared between two groups.Results After treatment,the total effective rate of treatment group was 96.72%,significantly higher than that of control group(86.89%)(P<0.05).After treatment,the scores of acid reflux,heartburn,and retrosternal pain in two groups were significantly decreased compared with those before treatment(P<0.05).After treatment,the typical symptom score of treatment group was significantly lower than that of control group(P<0.05).After treatment,HAMA,HAMD-17,GerdQ scores,and endoscopic scores were significantly decreased in both groups(P<0.05).After treatment,HAMA,HAMD-17,GerdQ scores,and endoscopic scores in treatment group were lower than those in control group(P<0.05).After treatment,the total number of reflux,the longest reflux time and the number of orthostatic acid reflux,liquid reflux and mixed reflux were significantly reduced in both groups(P<0.05).The improvement was more significant in treatment group(P<0.05).Conclusion Ganhai Weikang Capsules combined with esomeprazole sodium has good clinical efficacy in treatment of reflux esophagitis,and can significantly improve clinical symptoms and esophageal mucosa status,and significantly improve mental and psychological status and life quality,which is worthy of clinical application.
Objective To explore the clinical study of Shiwei Hezi Pills combined with piperazine ferulate in treatment of chronic glomerulonephritis.Methods A total of 136 patients with chronic glomerulonephritis admitted to Jiangsu Shengze Hospital Affiliated to Nanjing Medical University from January 2021 to March 2023 were selected and divided into control group(68 cases)and treatment group(68 cases)according to numerical random method.Patients in the control group were po administered with Piperazine Ferulate Tablets,200 mg/time,three times daily.Patients in the treatment group were po administered with Shiwei Hezi Pills on the basis of the control group,4 pills/time,three times daily.Patients in two groups were treated for 28 d.After treatment,the clinical evaluation was evaluated,and the symptom relief times,the levels of BUN,SCr,24 h urine,IL-6,TNF-α,VEGF and MMP-9,and adverse reaction in two groups before and after treatment were compared.Results After treatment,the clinical effective rate of the treatment group was 98.53%,which was significantly higher than that of the control group(85.29%,P<0.05).After treatment,the relief time of symptom in the treatment group was significantly earlier than that in the control group(P<0.05).After treatment,the indexes of BUN,SCr,24 h Upro,IL-6,TNF-α and VEGF in two groups were significantly decreased,while the level of MMP-9 were significantly increased(P<0.05),and these indexes in the treatment group were significantly better than those in the control group(P<0.05).After treatment,the incidence of adverse events in the treatment group(4.41%)was significantly lower than that in the control group(10.29%,P<0.05).Conclusion Piperazine ferulate combined with Shiwei Hezi Pills has a definite therapeutic effect,can obviously improve the clinical symptoms,regulate the state of renal function,weaken the inflammatory reaction,and is safe and effective.
Objective To study the effect of Wuzi Yanzong Pills on the differentiation of bone marrow hematopoietic stem cells to megakaryotic lineage in mice with myelosuppression caused by cyclophosphamide.Methods A model of chemotherapy-induced myelosuppression was constructed by intraperitoneal injection of cyclophosphamide into C57BL/6J mice.According to random number table method,the mice were divided into control group,model group,and Wuzi Yanzong Pills 4,8,16 g/kg groups,with 10 mice in each group.Drug intervention was started after the first injection of cyclophosphamide,and the control group was given normal saline intragaically.Wuzi Yanzong Pills group was given 4,8,16 g/kg Wuzi Yanzong Pills solution intragastrically,and continued for 3 weeks.On day 1,7,14 and 21 of the experiment,body mass was measured,orbital blood was taken,and 20 μL was absorbed into diluent solution dissolved in blood cell detector,and blood cell count was detected by automatic blood cell analyzer.HE staining of femur was used to observe the morphology and quantity of megakaryocytes.Hematopoietic stem and progenitor cell count of bone marrow were detected by flow cytometry,and megakaryocytes were sorted to determine the polyploidy of megakaryocytes.Results Wuzi Yanzong Pills can significantly increase the number of peripheral blood plates in myelosuppressed mice,promote the gradual differentiation of hematopoietic stem cells into megakaryocytes and promote the polyploid formation of megakaryocytes under dose correlation(P<0.05).Conclusion Wuzi Yanzong Pills can restore the bone marrow megakaryotic lineage damage caused by cyclophosphamide to some extent,promote the differentiation of the bone marrow hematopoietic system toward the megakaryotic lineage,and increase the production of endogenous platelets.
Objective To establish thedetailed rules for evaluating drug utilization of levothyroxine sodium tablets by AHM-TOPSIS method to provide references for its clinical rational application.Methods Establishing the drug utilization review standard of levothyroxine sodium tablets.According to this standard rules to analyze and evaluate the rationality of 276 prescriptions with levothyroxine sodium tablets from Jan.2021 to Mar.2023 by AHP-TOPSIS method.Results Among 276 medical records evaluated by levothyroxine sodium tablets.There were 1 cases(0.36%)with Ci≥90%,28 cases(10.14%)with Ci 80%—90%,49 cases(17.75%)with Ci 70%—80%,141 cases(51.09%)with Ci 60%—70%,50 cases(18.12%)with Ci 50%—60%,and 7 case(2.54%)with Ci<50%.The irrational use of levothyroxine sodium tablets was mainly reflected in aspects such as administration time interval,administration time,laboratory examination,dosage,indications,and indications.Conclusion The rationality evaluation method of clinical application of levothyroxine sodium tablets is established by AHM-TOPSIS method,and the method is feasible.
Objective To investigate the clinical effect of Xiaoer Qingre Lifei Oral Liquid combined with Cefixime Granules in treatment of acute bronchitis in children.Methods Children(96 cases)with acute bronchitis in Beijing Tongzhou Maternal and Child Health Hospital from January 2021 to December 2022 were divided into control and treatment groups according to random number table method,and each group had 48 cases.Children in the control group were po administered with Cefixime Granules,twice daily,each dose was 3.0 mg/kg,for children with body weight≥30 kg with the dose 100 mg/time.Children in the treatment group were po administered with Xiaoer Qingre Lifei Oral Liquid on the basis of the control group,three times daily,1—2 years old:5 mL/time,3—5 years old:10 mL/time,6 years old and above:15 mL/time.Children in two groups were treated for 7 d.The clinical efficacies,resolution time of main symptoms,respiratory function,and the serum factors in two groups were compared.Results After treatment,the total effective rate in the treatment group was significantly higher than that in the control group(P<0.05).After treatment,the regression time of fever,cough,phlegm,and pulmonary rales in the treatment group was significantly shorter than those in the control group(P<0.05).After treatment,the tidal volume in two groups was significantly increased,but the respiratory ratio and respiratory frequency in two groups were significantly decreased(P<0.05),and the tidal volume in the treatment group was higher than that in the control group,but the respiratory ratio and respiratory frequency were lower than those in the control group(P<0.05).After treatment,the serum levels of IL-2 were significantly increased,but the serum levels of CRP and TNF-α were significantly decreased(P<0.05).And the serum levels of IL-2 in the treatment group was higher than that in the control group,but the serum levels of CRP and TNF-α were lower than those in the control group(P<0.05).Conclusion Xiaoer Qingre Lifei Oral Liquid combined with Cefixime Granules can improve the therapeutic effect of acute bronchitis in children,improve the efficiency of symptom improvement,improve respiratory function,and reduce the degree of inflammation.
Objective To investigate the effects of edaravone on neural myelin regeneration,cognitive function,and the VEGF/Notch1 signaling pathway in rats with cerebral infarction.Method 50 rats were randomly divided into control group,model group,aspirin group,and edaravone 1.5,3 mg/kg group,with 10 rats in each group.Except for the control group,all other groups of rats established models of middle cerebral artery infarction.Edaravone 1.5,3 mg/kg groups were injected with edaravone injection through the tail vein,while the aspirin group was injected with aspirin 4 mg/kg solution.Observation of cognitive function of rats in each group through shuttle box combined with water maze experiment.ELISA method was used to detect the serum MBP content of rats in each group,Pal Weigert staining was used to observe the morphology of nerve myelin sheaths in rats of each group.TTC staining was used to measure the volume of cerebral infarction in each group of rats.Immunoblotting was used to detect the levels of VEGF/Notch1 in each group of rats.Results Compared with the model group,edaravone groups showed an increase in the number of active evasions and a decrease in the number of charges,learning and memory latency(P<0.05).Compared with the model group,edaravone groups showed a decrease in serum MBP content,infarct volume,and Notch1 level,while the VEGF level increased(P<0.05).The myelin sheath of the nerve fibers in the inner capsule of the control group was stained dark blue and the color was clear,and the background was extremely light yellow.Myelin disintegration,destruction and loss were serious in the model group,myelin sheath was sparse,myelin sheath breakage and loss were found in some areas,and the staining was light.The myelin morphology of edaravone and aspirin groups was improved.Conclusion Edaravone can promote VEGF activity,inhibit inflammatory response,regulate Notch1 signaling pathway,reduce MBP protein expression,promote nerve myelin regeneration,and enhance cognitive function.
Objective To optimize the preparation of paclitaxel/low molecular-weight heparin-cystamin-α-tocopherol succinate(PTX/LMWH-cys-TOS)micelles,and to evaluate its pharmaceutical property and in vitro cytotoxicity.Methods Using drug loading rate and encapsulation rate as indicators,the influence of organic solvent and drug carrier ratio on micelle drug loading capacity were investigated,and the optimal prescription was optimized.The freeze-drying method was used to prepare micelle freeze-dried powder injection and investigate their re-dissolution stability.The release characteristics of micelles in different concentrations of dithiothreitol solution were studied using dialysis method.The anti-tumor activity of micelles on paclitaxel sensitive and resistant cells was studied using CCK8 method.The concentration of paclitaxel in tumor cells was determined by HPLC method.Results The optimal ratio of paclitaxel to LMWH-cys-TOS was 1:3.6,using ethanol as the organic solvent.Micelle products with freeze-dried protectants had a good appearance,and the re-dissolved micelles should be stored at 4℃,and used as soon as possible.In the release medium of dithiothreitol,the release rate of paclitaxel increased with the increase of dithiothreitol concentration.When the concentration of dithiothreitol was 20 mmol/L,the cumulative release of paclitaxel after 36 h was as high as 93%.Micelles could enhance the toxicity of paclitaxel on drug-resistant cells HCT-15/Taxol and the concentration of the drug in tumor cells.Conclusion LMWH-cys-TOS micelles can improve the solubility of paclitaxel and achieve targeted drug delivery.
Objective To investigate the efficacy of Colistimethate Sodium for injection combined with Xuebijing Injection in treatment of sepsis.Methods Patients(104 cases)with sepsis in ICU of Fuling Hospital Affiliated to Chongqing University from March 2021 to February 2023 were divided into control and treatment groups using the random number table method,and each group had 52 cases.Patients in the control group were iv administered with Xuebijing Injection,100 mL added into 0.9%sodium chloride injection 100 mL,three times daily.Patients in the treatment group were iv administered with Colistimethate Sodium for injection on the basis of the control group,2.5 mg/kg,the dosage was divided into 2 to 4 doses,with the maximum dose not exceeding 5 mg/kg,in patients with kidney injury during treatment,the dose was reduced to half of the required daily dose,and the injection frequency was once per 12 h.Patients in two groups were treated for 7 to 10 d.The clinical efficacies,state of illness,and serum inflammatory factors were compared between two groups.Results After treatment,the total effective rate of the treatment group reached 92.31%,the total effective rate of the control group was 76.92%,and the total effective rate of the treatment group was higher than that of the control group(P<0.05).After treatment,APACHE Ⅱ score and SOFA score were significantly decreased in two groups(P<0.05),and APACHE Ⅱ scores and SOFA scores in the treatment group were lower than those in the control group(P<0.05).After treatment,the serum levels of CRP,PCT,and IL-6 were significantly decreased in two groups(P<0.05),and the serum inflammatory factors levels in treatment group were lower than those in control group(P<0.05).The incidence of kidney injury in the treatment group was higher than that in the control group(P<0.05).Conclusion Colistimethate Sodium for injection combined with Xuebijing Injection in treatment of sepsis can improve antibacterial effect,significantly improve the condition and reduce the inflammatory response of the body,but it will increase the risk of kidney injury during treatment.It is recommended to monitor the kidney function during treatment.
Objective Based on network pharmacology and molecular docking methods,the active components,potential targets and mechanism of Xiaoer Fengreqing Mixture(Oral Liquid)in the prevention and treatment of swine flu were screened.Methods Firstly,the composition data and prediction target of Xiaoer Fengreqing Mixture(Oral Liquid)were obtained from TCMSP and BATMEN-TCM database,and the treatment target of H1N1 was obtained from GeneCards database.Then,the function of the potential target is revealed by functional enrichment,and the"medicine-active ingredient-potential target"network is constructed by using Cytoscape software,and the protein interaction network of the potential target is constructed based on the STRING database.Finally,the molecular docking verification was carried out between the key targets and components.Results A total of 192 active components and 141 potential targets of Xiaoer Fengreqing mixture(oral liquid)were screened.Based on the"medicinal material-active ingredient-potential target"network and molecular docking technology,17 key active components were selected.The top core targets were prostaglandin endoperoxide synthase 2(PTGS2),heat shock protein(HSP90AA1),prostaglandin endoperoxide synthase 1(PTGS1),nitric oxide synthase(NOS2),peroxisome proliferator activated receptor gamma(PPARG)and adrenergic receptor β 2(ADRB2).A total of 2747 GO items were screened and enriched to 176 pathways related to drug prevention and treatment of H1N1.The preventive and therapeutic effect of Xiaoer Fengreqing Mixture(Oral Liquid)is mainly related to external stimulus response,signal transduction,oxidative stress and virus infection pathway.The results of molecular docking showed that the binding between the core target and the active component was stable.Conclusion Xiaoer Fengreqing Mixture(Oral Liquid)may play a role in the prevention and treatment of swine flu by inhibiting the proliferation of influenza A virus,inhibiting inflammation and regulating immunity.
Objective Exploring the clinical efficacy of armillarisini anatricome combined with cefazolin sodium in treatment of acute exacerbation of chronic cholecystitis.Methods The clinical data of patients(86 cases)with acute exacerbation of chronic cholecystitis in Jiaozuo People's Hospital from June 2020 to December 2022 were analyzed retrospectively.They were divided into control and treatment group,and each group had 43 cases based on different treatments.Patients in the control group were iv administered with Cefazolin Sodium for injection,2 g added into normal saline 100 mL,once every 12 h.Patients in the treatment group were iv administered with Armillarisini Anatricome for injection on the basis of the control group,5 mg added into normal saline 100 mL,once daily.Patients in two groups were treated for 10 d.After treatment,the clinical evaluation was evaluated,and the improvement time of symptom,the levels of serum CRP,CCK,IL-6,NF-κB,TNF-α,AST,ALT,and TBA in two groups before and after treatment were compared.Results After treatment,the clinical effective rate of the treatment group(97.96%)was significantly higher than that of the control group(83.67%,P<0.05).After treatment,the improvement time of right upper abdominal pain,nausea and vomiting,bitterness and abdominal distension in the treatment group was earlier than that in the control group(P<0.05).After treatment,the serum levels of CRP,CCK,IL-6,NF-κB,TNF-α,AST,ALT,and TBA were significantly decreased in two groups(P<0.05),and these serological indexes in the treatment group were significantly lower than those in the control group(P<0.05).Conclusion The combination of armillarisini anatricome and cefazolin sodium in treatment of acute exacerbation of chronic cholecystitis can promote the improvement of clinical symptoms,reduce liver damage and reduce inflammatory reaction.
