目的:研究芍药苷对体外小鼠脾细胞增殖及细胞因子的作用,探讨其对细胞免疫功能的影响及机制.方法:昆明种小鼠颈椎脱臼处死,体外培养小鼠脾细胞,不同质量浓度的芍药苷溶液(62.5,12.5,250,500,1 000 mg·L-1),另设空白组,分别于培养3,6,9d时取出细胞培养板,采用血球计数板计数细胞数法测定不同浓度芍药苷对小鼠脾细胞增殖活性的影响,酶联免疫吸附法(ELISA)测定培养上清中白细胞介素-2(IL-2),干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)的含量;作用9d后取细胞,Western blot方法测定IL-2,IFN-γ,TNF-α和核因子-κB(NF-κB)蛋白表达;500 mg·L-1芍药苷溶液作用5d后,流式细胞仪(FCM)检测细胞表型变化.结果:在3,6,9d,不同浓度芍药苷给药组的脾淋巴细胞增殖活性均高于空白组(P＜0.01);各给药组IL-2,IFN-γ,TNF-α的含量较空白组明显升高(P＜0.05,P＜0.01),并随芍药苷浓度的增加而增加;淋巴细胞表型分析显示,芍药苷能够刺激T淋巴细胞和B淋巴细胞增殖;不同质量浓度芍药苷能够升高IL-2,IFN-γ,TNF-α和NF-κB蛋白表达(P ＜0.05,P＜0.01).结论:芍药苷可通过激活NF-κB表达,促进细胞因子IL-2,IFN-γ和TNF-a的分泌和表达,促进细胞增殖,提高机体免疫功能.

本文通过德国无国界兽医组织在索马里进行的乳品质量提升项目的经验,探讨乳品价值链沿线多群体收入增加的推动模式,以期发现价值链、性别敏感和社区管理等发展理念在实践中的用武之地。

白内障超声乳化手术使得广大糖尿病患者术后获得了良好视力,但作为一个特殊高危群体,手术后并发症不容忽视。本文结合以往我科临床经验,按照发生率的高低,就糖尿病患者白内障术后并发症的发生情况作一剖析,并参照相关文献,讨论分析其发生的危险因素,及在临床工作中如何预防或减低糖尿病患者白内障术后并发症的发生几率,以期警示临床进一步加强围手术期管理制度,针对患者具体情况,依据临床专业理论和经验,作出最优化临床决策。

根据生产实践,采取在罐体内加水,隔离空气与可燃源,同时保证不让水漏出的焊接工艺方法,可以解决油水罐车罐体焊接难题。油水罐车罐体采用优质低碳钢,焊接性能比较好,用常规焊接方法即可达到焊接要求,不会出现焊接裂纹等缺陷。该焊接工艺不但能用在车载油水罐上,也可以用在其他压力不大的大罐补漏焊接上,有效防止了焊接爆炸事故的发生,为安全生产提供了保障。

目的研究乙肝患者外周血T细胞亚群的变化,进而了解其细胞免疫状态水平。方法用流式细胞仪对228例不同乙肝患者的外周血T细胞亚群CD+3、CD+4、CD+8绝对细胞计数及CD+4/CD+8比值进行检测,并将检测结果与e抗原、HBV-DNA含量、YMDD变异情况进行比较,以探讨各组之间的关系。结果与正常对照组相比,急性乙型肝炎(AHB)患者外周血T细胞亚群CD+3、CD+4、CD+8绝对细胞数升高(P<0.01);而慢性乙型肝炎(CHB)、肝硬化(LC)、原发性肝癌(PHC)患者依次降低,以LC、PHC两组降低最为显著(P<0.01);CD+4/CD+8比值AHB、CHB、LC、PHC 4组有依次升高的趋势。同时e抗原(-)、HBV-DNA(-)、YMDD变异株乙肝患者外周血T细胞亚群绝对细胞数明显低于e抗原(-)、HBV-DNA(-)、YMDD野生株乙肝患者(P<0.01或P<0.05);而CD+4/CD+8比值均高于对照组。结论不同乙肝患者体内存在T细胞亚群失衡及细胞免疫功能紊乱。

目的分析乙型肝炎患者HBV核苷(酸)类似物耐药相关的10个位点的突变情况及其临床意义。方法采用焦磷酸测序法对658例各型乙型肝炎患者行核苷类似物抗乙肝病毒多位点耐药基因检测并分析不同核苷(酸)类似物耐药的突变形式,对常见突变模式者ALT和HBV-DNA水平进行比较。结果 300例发生不同位点变异,变异率为45.59%,其中有明确用药史者202例。拉米夫定耐药突变中以M204V和M204I最常见,前者通常伴随L180M突变,后者常单独出现;阿德福韦酯耐药中以N236T和A181位碱基替换为主;恩替卡韦耐药均伴随拉米夫定和阿德福韦酯耐药变异,以T184位点的碱基替换最为常见;主要变异模式间ALT及HBV-DNA水平均无明显差异。90例未接受过核苷(酸)类似物治疗者亦检出耐药相关突变。结论检测HBV多位点耐药基因相关突变有助于临床及时发现和确认乙型肝炎患者HBV耐药并指导抗病毒治疗。

目的观察碘伏用于早产儿培养箱消毒效果。方法在母婴同室内,使用含量为250 mg/L碘伏溶液擦拭消毒后,对培养箱内空气、表面、操作窗口做细菌监测。结果碘伏擦拭消毒后培养箱有机玻璃罩内壁、贮水口、床垫、操作窗检测细菌数分别为1.25、3.10、2.23和2.25 cfu/m2,消毒后24 h,检测细菌数分别为3.25、4.16、6.2、7.45 cfu/m2。结论碘伏毒性低、无刺激性,性能稳定,适合对早产儿培养箱进行消毒。

目的探讨学生对"学生讲课,教师指导"的互动式英语教学方法的赞同情况及其存在的问题。方法选取医学硕士研究生英语读写班122名学生为教学对象,对其进行"学生讲课,教师指导"的互动式英语教学方法,采用自行设计的问卷调查学生对该教学方法的评价。结果 79名(64.8%)学生赞同并接受"学生讲课,教师指导"的互动式教学方法,主要原因为该教学方法可以提高学生独立思考和解决问题的能力;31名(25.4%)学生不赞同这种教学方法,主要原因为学生讲课重点不突出,分不清主次;12名(9.8%)学生对这种教学方法表示无所谓。结论 "学生讲课,教师指导"的互动式英语教学方法存在一些亟待解决的问题,但比较适合医学研究生研究性学习。

在大型油罐建设中,浅层土为高压缩、软弱土层,下部为液化砂层的地基较难处理。针对该类型场地提出了采用强夯置换法地基处理方案。实践证明,该方案较为可行,并取得了满意的效果。

试验选取30日龄的云南茶花鸡、武定鸡、盐津乌鸡、AA肉鸡和土杂鸡等96只鸡,随机分为3组,分别饲喂3种不同的蛋白水平日粮,即16.5%、18%和19.5%,探讨日粮蛋白水平对30～60日龄的云南部分地方鸡、肉鸡及土杂鸡内脏器官指数的影响。试验结果表明:品种对各内脏质量及其指数均有显著的影响。肉鸡和土杂鸡的内脏器官质量显著高于云南地方鸡种,而地方鸡的总内脏指数、肠指数、胃指数、肝指数、心脏指数和肺指数均高于肉鸡和土杂鸡（P＜0.05）。品种间内脏指数随活质量的降低而增加。日粮蛋白水平对内脏质量及其指数的影响不显著。

Objective To investigate the expressions of tumor M2 pyruvate kinase (Tu M2-PK) and to explore the clinic significance in lung squamous cancer.Methods Specimens from 28 cases with lung squamous cancer and 21 cases in control group their Tu M2-PK protein expressions were detected by immunohistochemical method,and the correlation between the Tu M2-PK protein expressions and clinical pathological paramter was analyzed statistically.Results The Tu M2-PK protein expressions were 82.14% (23/28) in lung squamous cancer,and 4.76%(1/21) in non cancer lung tissues,respectively(P<0.05). The positive expressions of Tu M2-PK protein were strongly correlated to the lung squamous cancer histological classifications and lymphoid metastasis (P<0.05),while it was no significant relationship to the sex and age(P>0.05).Conclusion Tu M2-PK protein expressions may be used as a marker to diagnose lung squamous carcinoma. It can be used as a predict mark for prognosis of lung squamous cancer.

Through the survey,interviewing,documentation and other methods,the paper investigated the daily lives of community residents of changchun,in order to improve their bad way of life and train residents of scientific,health,sports and civilized way of life and promote the National Fitness Program to smooth implementation.

<正>患者女,22岁,因舌尖部无痛性肿块于2007年2月13日来我院就诊。查体:舌尖部有直径约2cm的肿物,表面隆起,呈粉红色,界限清楚,无压痛,触之实性,质地稍硬,活动。治疗:局麻下行舌肿物扩大切除术,伤口I期愈合。病理检查:

数据挖掘技术主要用于对数据库中的海量数据进行有效的处理。为了进一步提高数据挖掘算法的效率,统计学中的抽样思想被引入到数据挖掘中。首先对数据挖掘和抽样调查做了简要概述,阐述了抽样在数据挖掘中的重要作用;然后进一步分析抽样在不同领域数据挖掘中的应用,并对其在数据挖掘应用中存在的问题进行了讨论。

AIM:To investigate the gene expression profile of human lung squamous cell carcinoma in early stage.METHODS:The mRNA was extracted from cancer tissue and normal lung tissue.The mixed probes were labeled and hybridized with chip containing 480 carcinoma related genes,then analyzed by SuperArray Image software to study the gene expression patterns in lung squamous cell carcinoma.RESULTS:192 genes associated with lung squamous cell carcinogenesis were found,which were grouped into transporter,adhesion molecules,cytoskeleton proteins,transcription regulator,genes associated with metabolism and immune response according to functions.127 gens were positive to lung squamous cell carcinogenesis and 65 genes were negative to lung squamous cell carcinogenesis.CONCLUSION:The screen of gene expression profile of human lung squamous cell carcinoma provides valuable information for the research of molecular mechanism of the lung squamous cell carcinogenesis.

To investigate effects of breeds(genotypes) and diets nutrient levels on the meat quality performance of Yunnan native chicks during 30 ～ 60 days old, a total of 480 chicks of five breeds(Chahua chickens,Wuding chickens,Yanjing chickens,Hybrid chickens and AA broilers) were assigned to feed on three dietary treatments(CP:17.50 %,18.00 % and 19.50 %) with arranging three groups randomly.The results showed:(1)There were significant differences between histolgic characteristic of muscle and the Yunnan native chicks had smaller diameter of breast and limb muscle fiber(P < 0.05) and larger density of breast muscle fiber(P < 0.05) than that of Hybrid chickens and AA broilers.(2)There were no significant differences on histolgic characteristics of muscle in diets with different protein levels,and the breast muscle fiber diameter and density of the diet Ⅱ were higher than the other two diets(P﹥0.05).(3)There were significant differences from the chest muscle meat quality between five chicks and the tenderness of Yunnan native chicks obviously higher than that of Hybrid chickens and AA broilers(P < 0.05).(4)The chest muscle meat quality of the chicks feeding higher diets were better than that of low protein diets(P﹥0.05).The results suggested that meat quality characteristics of Yunnan native chicks(Chahua chickens,Wuding chickens,Yanjing chickens) were better than that of Hybrid chickens and AA broilers and increasing protein levels,the chest muscle meat quality and the muscle tenderness were improved.

我科2004年3月至2006年12月进行超声乳化摘出及人工晶状体植入术治疗44例(56眼)2型糖尿病性白内障,疗效良好,现报告如下.

The advancement of phacoemulsification gives a hand to all the diabetic patients;however,the challenge of surgery cannot be neglected for the relatively high-risk group.By combining the past clinical experience to practice,this paper tries to discuss the importance and necessity of risk evaluation surrounding the cataract surgery in diabetes and wishes to alert all the surgeons to rigidly master the accordance of surgery and make sufficient risk evaluation before operation.Moreover,at the same time,it practically set down one perfect clinical decision-making according to individual conditions.

人无细胞真皮基质(ADM)能够成为永久性真皮替代物的主要原因为其具有低免疫原性、血管化能力及能够作为宿主细胞的有效载体,这些特性和ADM组成成分有关.近期,我们分别采用高渗盐-SDS法、DispaseⅡ-Triton法、高渗盐-NaOH消蚀法制备人ADM,并对其优缺点进行了比较,旨在为临床应用提供依据.