This study aimed to explore the distinct metabolic profiles and potential mechanisms induced in endothelial cells stimulated by serum from patients with dyslipidemia presenting with Phlegm-Dampness Retention Syndrome (PDRS) and spleen and kidney yang deficiency syndrome (SKYDS), using LC-MS-based metabolomics. LC-MS-based metabolomics was employed to analyze the metabolic profiles of human umbilical vein endothelial cells (HUVECs) exposed to serum from dyslipidemia patients with PDRS or SKYDS. This study found that (1) No statistically significant differences (p > 0.05) were observed in the general characteristics or disease severity between dyslipidemia patients with PDRS and those with SKYDS. (2) Principal Component Analysis (PCA; PC1 = 44.5%, PC2 = 14%, PC3 = 12.3%) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA; t[1]p = 23.8%, t[1]O = 26.6%) indicated clear separation between the PDRS and SKYDS groups. (3) Four representative differential metabolites were identified by LC-MS, and enrichment analysis revealed seven significantly altered metabolic pathways involving multiple biochemical processes. HUVECs stimulated by serum from dyslipidemia patients with PDRS and SKYDS exhibit distinct metabolic profiles. The seven significantly altered pathways may underlie the differential metabolic mechanisms associated with these syndromes. TRIAL REGISTRATION: China Clinical Trial Registration Center: ChiCTR2100046722.

Objective: Erchen decoction, a traditional Chinese medicine formula, can reduce the level of oxidative stress for the treatment of dyslipidemia phlegm-dampness retention syndrome (DPDRS); however, studies have not elucidated the mechanism underlying its metabolic action. Here, liquid chromatography-mass spectrometry (LC-MS)-based metabolomic techniques were utilized to characterize the in vivo effects of Erchen decoction in achieving reduction of oxidative stress levels and understand the potential metabolic mechanisms of action. Methods: We constructed a DPDRS animal model using a multifactorial composite modeling approach, and Erchen decoction was administered by gavage. We employed LC-MS-based metabolomic techniques in combination with serum-associated factors, gene transcription, methylation detection, and hematoxylin and eosin staining. Results: In this study, the constructed animal model of DPDRS had satisfactory quality. Erchen decoction treat-ment reduced the levels of low-density lipoprotein cholesterol, t total cholesterol and riglyceride; it improved the endothelial structure, increased levels of serum beta-nicotinamide adenine dinucleotide phosphate and glutathione concentrations, increased aortic phosphoserine aminotransferase and phosphoserine phosphatase gene expression levels, and decreased aortic phosphoglycerate dehydrogenase methylation level. A total of 64 differential metabolites were obtained using LC-MS assay, and 34 differential metabolic pathways were obtained after enrichment. Conclusions: Erchen decoction treatment of DPDRS mice reversed lipid indexes, improved vascular endothelial structure, increased serum and aortic anti-oxidative stress factor concentration and expression levels, and decreased methylation levels, thereby reducing oxidative stress and protecting vascular endothelium. Tricar-boxylic acid cycle and metabolic pathways of serum glutamine, serine, tryptophan, pyrimidine, and pyruvate were the most relevant metabolic pathways involved in reducing oxidative stress levels by Erchen decoction during DPDRS treatment; especially, mitochondrial redox homeostasis maintenance in endothelial cells may be crucial. In this work, the therapeutic potential of Erchen decoction for reducing the oxidative stress level in DPDRS was demonstrated; however, its in-depth mechanism is worth further exploration.

目的 观察丹荷颗粒对高胆固醇血症大鼠肝脏脂质沉积及相关蛋白表达的影响,探讨其保护肝脏的作用机制.方法 36只雄性SD大鼠随机分为空白对照组、模型组、阳性药组(阿托伐他汀2.1 mg/kg)和丹荷高、中、低剂量组(丹荷颗粒3.30、1.10、0.37 g/kg),每组6只.除空白对照组外,其余大鼠予高脂乳剂灌胃12周+潮湿垫料饲养建立高胆固醇血症模型,空白对照组予纯净水灌胃+正常垫料饲养.第7周开始,造模同时各给药组予相应药物灌胃,空白对照组和模型组灌胃纯净水,至造模结束.计算大鼠肝重比,全自动生化分析仪检测血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)含量,油红O染色观察肝组织脂质沉积,透射电镜观察肝细胞超微结构,Western blot检测肝组织固醇调节元件结合蛋白1(SREBP-1)、肝X受体α(LXRα)、视黄醇X受体α(RXRA)蛋白表达.结果 与空白对照组比较,模型组大鼠体质量、肝重比和血清TC、LDL-C、HDL-C、ALT含量显著升高(P<0.05,P<0.01),油红O染色面积比显著增加(P<0.01),肝细胞内脂滴增多,细胞核变小,线粒体变形,内质网结构模糊,肝组织SREBP-1、LXRα、RXRA蛋白表达显著升高(P<0.05,P<0.01);与模型组比较,各给药组大鼠体质量、肝重比和血清TC、LDL-C、HDL-C含量显著降低(P<0.05,P<0.01),油红O染色面积比显著减少(P<0.05,P<0.01),丹荷各剂量组血清ALT含量显著降低(P<0.05,P<0.01),丹荷高剂量组肝细胞脂滴减少,细胞核大小适中,线粒体完整,内质网结构清晰,肝组织SREBP-1、LXRα、RXRA蛋白表达显著降低(P<0.01).结论 丹荷颗粒可降低高胆固醇血症大鼠血清胆固醇,减少脂质沉积,其作用机制可能与下调SREBP-1、LXRα、RXRA蛋白表达,抑制肝脏脂质合成相关.

目的 探讨不同浓度益气化痰活血法代表方剂调脂通脉颗粒对血脂异常痰浊阻遏证小鼠动脉粥样硬化相关炎性因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达的影响.方法 选取雄性ApoE基因敲除小鼠24只,均采用高脂饲料喂养方法构建血脂异常痰浊阻遏证的病-证模型.将造模成功小鼠随机分为模型组、调脂通脉颗粒低剂量组、调脂通脉颗粒中剂量组、调脂通脉颗粒高剂量组,每组6只.除模型组外,调脂通脉颗粒低、中、高剂量组分别给予2g/(kg·d)、4 g/(kg·d)、8 g/(kg·d)的调脂通脉颗粒灌胃4周.灌胃结束后,采用ELISA法检测各组小鼠血清IL-6、TNF-α水平.结果 ApoE基因敲除小鼠均无脱失.模型组、调脂通脉颗粒低剂量组、调脂通脉颗粒中剂量组、调脂通脉颗粒高剂量组小鼠血清IL-6和TNF-α水平均依次下降,差异均有统计学意义(P均<0.05);调脂通脉颗粒高剂量组血清IL-6水平均明显低于模型组、调脂通脉颗粒低剂量组、调脂通脉颗粒中剂量组(P均<0.05),血清TNF-α水平明显低于模型组、调脂通脉颗粒低剂量组(P均<0.05);调脂通脉颗粒低、中剂量组与模型组比较,调脂通脉颗粒低剂量组与调脂通脉颗粒中剂量组比较,血清IL-6和TNF-α水平差异均无统计学意义(P均>0.05).结论 调脂通脉颗粒能降低血脂异常痰浊阻遏证小鼠血清IL-6、TNF-α水平,其中高剂量调脂通脉颗粒可更明显下调IL-6水平,而TNF-α水平的降低对调脂通脉颗浓度的依赖程度不大.调脂通脉颗粒可能通过抑制动脉粥样硬化相关炎性因子的表达,从而减轻血脂异常痰浊阻遏证下血管内皮的炎症反应,延缓病变进展.

思想政治教育与专业课程教学的全面深入融合,是构建全员全程全方位育人格局的重要途径.基于OBE(outcome-based education)理念,结合中医诊断学的课程特点及内容,课程组深入挖掘课程思政元素,凝练课程思政内涵,并通过教学实践,不断完善中医诊断学课程的思政建设,逐步建立了中医诊断学实训课程思政的教育模式.为增强学生的职业使命感,提升学生的医学人文素养奠定基础,以达到全面育人的目的.

Phlegm-dampness retention (PDR) syndrome is one of the main syndromes of dyslipidemia. This study investigated the effects of Erchen decoction (ECD) on concentrations of two oxidative stress-related cytochrome P450 (CYP450) metabolites of arachidonic acid-14,15-dihydroxyeicosatrienoic acid (14,15-DHET) and 20-hydroxyeicosatetraenoic acid (20-HETE)-in mice with dyslipidemia and phlegm-dampness retention (PDR) syndrome (n = 5 C57BL/6J mice and n = 30 apolipoprotein E knockout mice). Murine models of the disease and syndrome were established using multifactor stimulation. Then, all mice were assigned to normal, model, low- (L-), medium- (M-), or high- (H-) dose ECD groups or to a control or an unmatched prescription-syndrome (unmatched P-S) group; five mice were included in each group. Dose formulations were administered by oral gavage for 30 days to animals in the corresponding groups. We detected and analyzed hematoxylin and eosin (HE) staining characteristics of the mouse aorta and serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), peroxynitrite (ONOO-), 14,15-DHET, and 20-HETE concentrations in each group. TC and LDL-C concentrations significantly decreased in the M-ECD versus control group (P < 0.05); however, the TC and LDL-C concentrations were not significantly different in the unmatched P-S versus model group (P > 0.05). After treatment in the P-S correspondence groups (L-ECD, M-ECD, and H-ECD groups), the concentration of ONOO- decreased to different degrees in each group. Among these groups, the concentration of ONOO- significantly decreased in the L-ECD, M-ECD, and H-ECD groups versus the model group (P < 0.05). However, the concentration of ONOO- was not significantly different in the unmatched P-S versus the model group (P > 0.05). From the perspective of aortic HE staining, the P-S group experienced an improved endothelium structure after treatment. 14,15-DHET concentrations significantly increased in the normal, M-ECD, and H-ECD groups versus the model group; in the H-ECD versus the L-ECD group; and in the H-ECD versus the control group (all P < 0.05) to various extents after different doses of the prescription. 20-HETE concentrations pronouncedly decreased in the M-ECD versus normal group; in the M- and H-ECD groups versus the model group; in the M-ECD versus the L-ECD group; in the M-ECD versus the control group; and in the M-ECD versus the unmatched P-S (P < 0.05). However, the concentrations of 14,15-DHET and 20-HETE in the model group were not significantly different from those of the unmatched P-S (P > 0.05). In this study, ECD reversed blood lipid indexes and ameliorated oxidative stress-related metabolites, elevating serum 14,15-DHET and lowering serum 20-HETE in mice with dyslipidemia and PDR syndrome via CYP450 pathways of arachidonic acid metabolism. The efficacy of ECD relies on correspondence between the prescription and the syndrome. These findings scientifically validate the treatment according to traditional Chinese medicine syndrome differentiation. ECD can strengthen the protective effect on the vascular endothelium by driving out pathogenic factors and strengthening healthy resistance. Its efficacy may be related to the adjustment of the polarization state of macrophages.

课程思政是新形势下对于高校各学科教育教学的要求.中医诊断学作为一门必修课、桥梁课,是学生从基础理论知识过渡到临床各科时的重要一环.加入课程思政内容,有利于学生认识"准医生"身份,提高职业道德素养,从而提高文化自信、行业自信.教师是课程思政的执行主体.培养教师课程思政的能力才能真正把教书与育人结合,切实担起育人职责.本文探讨了在中医诊断学教学实践过程中,教师应如何树立牢固的育德意识,提高育人的能力,真正在传授知识的同时进行思想价值引领,发挥"润物细无声"的教育作用.

目的 本研究将对线上混合式教学模式的现阶段应用进行深入解析与讨论.方法 通过对融合课程"中医辨证论治学基础"混合式教学改革实例进行剖析,形成有针对性的教学与评价方案.结果 "中医辨证论治学基础"课程建立了完整的线上[重点内容讲授+问题解答+案例讨论+互动评价+大规模开放式在线课程(Massive open online course,MOOC)学习+小规模限制性在线课程(Small private online course,SPOC)讨论]混合式教学模式,并通过线上问卷星教学效果评价(整体试卷分析+案例题分析)、SPOC作业评价(教师评价+学生互评),形成了教师教学与学生学习评价的双向反馈机制.结论 线上混合式教学模式的应用,需要提高对学生主体自主学习意识的激发,更新教师教学方法、教学手段以适应新环境下的教学任务,并通过多元评价体系的引入及时有效地调整混合式教学模式下师生双方的教学体验.

中医临床中,将"疲劳"感受常描述为"倦怠""乏力""懈怠""困倦""神疲"等.多将其归于气虚、气血两虚、气阴两虚、阳虚或暑淫、湿阻等证中,被认为是这些证(证是中医认识疾病的一种基本模式,证表现为一组相关的症状)的一种临床表现.当气虚、气血两虚、阳气不足时,精神、肌肉、骨骼、筋脉得不到充养,机体的活力得不到调动,就会出现"精神困倦""肢体懈怠乏力或酸软"的情况.

Background : ‘Treating the same disease with different methods’ is a Traditional Chinese Medicine (TCM) therapeutic concept. That means although patients are diagnosed with the same disease, they may have different syndromes that require distinct drug administrations. This study aimed to identify the differentially expressed genes and related biological processes in dyslipidemia with the Phlegm-Dampness Retention (PDR) syndrome and the Spleen and Kidney Yang Deficiency (SKYD) syndrome using transcriptomic analysis. Methods: Ten ApoE knockout (ApoE-/-) mice were used for the establishment of dyslipidemic disease-syndrome models via multifactor-hybrid modeling, with 5 in the the PDR group and 5 in the SKYD group. Five C57BL/6J mice were employed as normal controls (NC) group. Test model quality. Aortic endothelial macrophages in mice were screened using flow cytometry. Transcriptomic analysis was performed for macrophages using RNA-Seq. Results: ①The quality assessment of the disease-syndrome model showed that TG, TC, and LDL-C levels significantly increased in the PDR and SKYD groups versus the NC group (P < 0.05). Combined with HE staining of aorta, the disease model was successfully established. ②The quality assessment of the syndrome models showed that mice in the PDR group presented with typical manifestations of the PDR syndrome, and mice in the SKYD group had the related manifestations of the SKYD syndrome, indicating that the syndrome models were successfully constructed. ③After comparing the differentially expressed gene (DEG) expressions in macrophages in dyslipidemia mice with different syndromes, 4142 genes were identified with statistical significance (P < 0.05). The Gene Ontology (GO) analysis for the DEGs showed that biological process of difference between PDR group and SKYD group include both adverse and protective processes were included. Conclusion: The DEGs between the PDR syndrome and the SKYD syndrome indicate different biological mechanisms between the onset of the two syndromes. They have distinctive biological processes, including adverse and protective processes, corresponding to the invasion of pathogenic factors into the body and the fight of healthy qi against pathogenic factors, respectively, in the TCM theory. Our results have demonstrated the biological evidence behind ‘treating the same disease with different treatments’ in TCM.

Background: “Treating the same disease with different methods” is a Traditional Chinese medicine (TCM) therapeutic concept suggesting that, while patients may be diagnosed with the same disease, they may also have different syndromes that require distinct drug administrations.Objective: This study aimed to identify the differentially expressed genes and related biological processes in dyslipidemia in relation to phlegm–dampness retention (PDR) syndrome and spleen and kidney Yang deficiency (SKYD) syndrome using transcriptomic analysis.Methods: Ten ApoE−/− mice were used for the establishment of dyslipidemic disease–syndrome models via multifactor-hybrid modeling, with five in the PDR group and five in the SKYD group. Additionally, five C57BL/6J mice were employed as a normal control group. Test model-quality aortic endothelial macrophages in mice were screened using flow cytometry. Transcriptomic analysis was performed for macrophages using RNA-Seq.Results: A quality assessment of the disease–syndrome model showed that levels of lipids significantly increased in the PDR and SKYD groups, compared to the normal control group, p < 0.05. Applying, in addition, hematoxylin and eosin staining of aorta, the disease model was also successfully established. A quality assessment of the syndrome models showed that mice in the PDR group presented with typical manifestations of PDR syndrome, and mice in the SKYD group had related manifestations of SKYD syndrome, indicating that the syndrome models were successfully constructed as well. After comparing the differentially expressed gene expressions in macrophages of the dyslipidemic mice with different syndromes, 4,142 genes were identified with statistical significance, p < 0.05. Gene ontology analysis for the differentially expressed genes showed that the biological process of difference between the PDR group and the SKYD group included both adverse and protective processes.Conclusion: The differentially expressed genes between PDR syndrome and SKYD syndrome indicate different biological mechanisms between the onsets of the two syndromes. They have distinctive biological processes, including adverse and protective processes that correspond to the invasion of pathogenic factors into the body and the fight of healthy Qi against pathogenic factors, respectively, according to TCM theory. Our results provide biological evidence for the TCM principle of “treating the same disease with different treatments.”

目的:分析肝炎肝硬化患者中医症状与终末期肝病模型(MELD)评分的相关性,为临床通过症状判断肝炎肝硬化患者病情轻重提供依据.方法:运用《肝炎肝硬化临床信息采集表》采集752例肝炎肝硬化患者的中医症状信息,并按统一标准计算患者的MELD评分.分别按照是否出现某种症状或某种症状的轻重程度对752例患者进行分组,比较MELD评分在各组间的差异,提取MELD评分差异有统计学意义(P＜0.05)的症状.对这些症状与MELD评分进行多元线性回归分析,建立回归方程,提取与MELD评分关系最为密切的症状.结果:共采集到752例肝炎肝硬化患者的97个症状.接是否出现某种症状或某种症状的轻重程度对患者进行分组,比较各组MELD评分的差异,共提取到差异有统计学意义(P＜0.05)的症状39个.对这39个症状与MELD评分的多元线性回归分析显示,11个症状与MELD评分关系最为密切,其中黄疸、面色青黄、呕吐、神疲、数脉、尿量减少、消瘦、面色黑、腹水与MELD评分呈正相关,胁痛、白苔与MELD评分呈负相关,MELD评分=4.267+4.921×黄疸+2.529×面色青黄+1.956×呕吐+1.477×神疲+1.197×数脉+0.946×尿量减少+0.797×消瘦+0.762×面色黑+0.704×腹水-1.456×胁痛-1.055×白苔.结论:肝炎肝硬化患者的中医症状与MELD评分存在一定关联,随着MELD评分的不断增高,患者的大部分中医症状亦越来越突出,为临床通过中医症状判断肝炎肝硬化患者的病情轻重提供了一定参考.

目的 本研究将深入解析职院诊断学课程实训教学与形成性评价方案评价方法.方法 通过对形成性教学评价方法特点的解析,形成有针对性的形成性考核方案.结果 中医诊断学实训课程形成性评价与教学效果等存在密切联系.结论 中医诊断学课程具有操作性强、案例带入性强等特点,需要逐教学单元进行分层评估,如师生评估、学生互评等等.

中医诊断学课程的内容可概括为诊法与辨证两大部分,因此,对其MOOC(Massive Open Online Course)的建设与实践分为中医诊断学MOOC(上)和中医诊断学MOOC(下)两部分进行.基于混合式学习和探究性学习的先进理念,以及望、闻、问、切四诊的教学目标和教学内容特点,首先将导论及四诊部分内容碎片化,重新梳理为118个知识点,制成118个短小授课视频,并设计了非视频内容(如作业、讨论、考试等),形成中医诊断学MOOC(上)的课程内容,在中国大学MOOC平台免费开课8轮,选课人数17万余人,实现了课程的校内、校外共享与应用,促进优质教学资源的共享,并为全面开展翻转课堂提供支撑.

目的 通过对文献的研究,探讨干预血脂异常的药食同源中药的用药特点,并对其进行病机分析.方法 检索中文数据库(知网、万方、维普)筛选2008—2018年间血脂异常的临床文献,借助"中医传承辅助平台V2.5",以2018年卫健委公布的110种药食同源中药为对照,对数据进行频次、关联规则分析,按通用教材对药食同源中药的药效、性味和归经进行统计分析,基于统计结果 探讨药食同源中药干预血脂异常的用药特点,并对其进行病机分析.结果药食同源中药63味(占常用209味的30.1％),出现频次为1750次(占209味中药出现次数的39.0％);出现频次≥20次的药食同源中药共18味(占63味药食同源中药的28.6％),出现频次为1510次(占209味药食同源中药出现次数的86.3％),按频次由高到低排序依次为山楂、茯苓、决明子等;发掘出10组治疗血脂异常的药食同源中药组合;干预血脂异常的药食同源中药药味以甘为主,药性以平为主,归脾经的药物最多.结论 药食同源中药干预血脂异常多以健脾为基本治则,兼以祛湿化痰、活血化瘀,常用药食同源中药为山楂、决明子、茯苓等,可将这些药物用于血脂异常的日常保健与临床防治.

问汗是《中医诊断学》问诊中重要的部分,汗出有无及特点在临床有很重要的指导意义.汗是津液的组成部分,为人体阳气蒸化津液达于体表而成,具有调节体温、滋润皮肤、排出废物等作用.如《素问?阴阳别论》说:"阳加于阴谓之汗"汗液分泌,虽资于阴精,运用于阳气,但还须依靠人体卫气之调节,腠理玄府开合适宜,才能汗出有度,从而保持体内之阴阳平衡.结合自己的教学体会,将教材问汗部分内容做相应调整,以问汗的要点:汗出有无,在疾病过程中,尤其是对外感患者,询问汗的有无,是判断感受外邪的性质和卫气盛衰的重要依据.汗出时间我们从两个方面来看,一个是昼夜时间点,一个是疾病发展变化时间点.按昼夜时间不同,常见的汗出类型有自汗、盗汗.疾病发展阶段变化时间点,我们谈战汗;汗出量多少,是汗出量的评价,大体可以划分无汗、微汗、汗多、大量汗出不同程度;汗出部位及兼证为纲目,清醒时经常汗出不止,活动之后更甚的表现称自汗;熟睡之后汗出,醒后汗止,称盗汗.盗汗主阴虚内热证;战汗是疾病病情发展的转折点,病势深重阶段,先见恶寒战栗,而后汗出者,使初学者便于理解和掌握.

自"形成性评价"的概念提出后,在教育界得到广泛重视,而"以学生为本"也是近年来提倡的教育理念,本课程组在"以学生为本"的视域下,基于中医诊断学学科结构和特点,并在符合形成性评价的目的和特征的基础上,以全面提升学生专业素质为目标,将学生学习积极性、主动性及学生赞成度、支持度、满意度等情况纳入到课程教学活动中,并体现在"形成性评价"中,构建了中医诊断学"形成性评价"模式,并对该模式包含的理论基础及目标构建进行了阐述,具体描述了"形成性评价"的评价方式及操作规程.

Objective To analyze the change rules of FibroScan detection value( FS value) of patients with hepatitis cirrhosis in different disease stages and levels,and its correlation with traditional Chinese medicine( TCM) disease syndrome elements. Methods A total of 108 patients with hepatitis cirrhosis were included,of which 69 were compensated and 39 were decompensated. The syndrome identification criteria were developed,and the disease syndrome elements of each patient were determined. The patient information was collected,and Child-Pugh scores on patients were performed. Then grade according to scores,and model for end-stage liver disease( MELD) scores were evaluated. FS value was detected. The FS values of patients with different stages and different Child-Pugh grades were compared,and the correlation between FS values and disease syndrome elements were analyzed. Results FS value in patients with compensated liver cirrhosis was significantly lower than that in patients with decompensation( P <0. 01). There was a positive correlation between FS value and Child-Pugh score,Child-Pugh grade as well as MELD score( P < 0. 01). The distribution of common syndrome elements in compensatory hepatitis cirrhosis patients was as follows: yin deficiency( 45 cases),qi stagnation( 43 cases),and yang deficiency( 39 cases). The distribution of common syndromes in patients with liver cirrhosis in decompensated stage was as follows: water retention( 34 cases),yin deficiency( 30 cases),and blood stasis( 29 cases). The distribution frequency of blood stasis,yang deficiency,qi deficiency and water retention was significantly different between the two groups( P < 0. 05). In the patients with liver cirrhosis at compensatory stage,the FS values of the patients with blood stasis,yin deficiency,damp-heat,yang deficiency and water retention were higher than those of non-appearance patients without above syndromes. The FS value of patients with qi stagnation was lower than that of patients without qi stagnation( P < 0. 05). In the decompensated patients,the FS value of patients with qi stagnation syndrome was lower than that of patients without qi stagnation. The FS values of patients with blood stasis,yin deficiency,damp-heat,qi deficiency,yang deficiency,and water retention were higher than those of patients without above syndromes. Among them,the comparison of whether or not blood stasis,water retention,and yang deficiency were statistically significant( P < 0. 05). Conclusion The FS detection value of patients with hepatitis and cirrhosis increase with the aggravation of the disease. There is a certain correlation between TCM syndrome elements and the FS detection value of the patients with liver cirrhosis. The patients with blood stasis,water retention,yang deficiency syndrome are more serious.

Objective: To analyze the application status of fatigue self-assessment scale (FSAS). Methods: On the basis of retrieving the literature reports of FSAS research, the contents of FSAS research were sorted out and analyzed by bibliometrics. Result: Thirty-eight literatures were included and analyzed, 21 of which related to the types, degrees and characteristics of fatigue,12 related to the evaluation of fatigue intervention effect, 4 related to the relationship between fatigue and TCM syndromes, and 1 related to the relationship between fatigue and TCM constitution. Conclusion: FSAS can better assess the type, degree and characteristics of fatigue in different groups, and reflect the correlation between fatigue and syndrome, physical fitness and the intervention effect of fatigue.

亚健康是人体处于健康和疾病之间的一种状态,疲劳性亚健康是以疲劳为主要表现的亚健康状态,是亚健康状态最常见的类型之一.近年来疲劳性亚健康的发生率急剧升高,对人们的生理和心理均有不同程度的影响,降低了人们的日常生活质量,同时也引起社会各界的广泛关注.由于目前各个领域对疲劳性亚健康缺乏统一的认识,给临床工作者、研究者及健康管理者准确、合理评价及干预该状态带来了一定困难.因此,为促进疲劳性亚健康的相关研究,文章通过对近10年疲劳性亚健康的相关定义、疲劳性亚健康的判定标准及其常见的中医证候进行系统梳理和总结,希望能进一步促进亚健康相关学科的研究,并为其提供一定的参考依据.