目的 构思和研制一种新型的急救与自救用防毒面具,用于解决肺刺激剂或肺损伤性毒剂中毒现场伤员的急救和现场人员自救使用.方法 通过在防毒面具上增加微型的供氧和药物雾化装置,设计形成三位一体化.结果 一体化设计使新型的急救与自救用防毒面具形成供氧与雾化供药的医疗救治功能,也具备了应急逃生者在无氧恶劣环境下应急供氧的逃生需求.结论 在肺刺激剂或肺损伤性毒剂中毒现场,使用新型的急救与自救用防毒面具,使得伤员的救治率和现场人员自救率大大提高.

化学战剂施放和有毒化工气体泄漏具有毒性强、作用快、毒效持久的特点,可使人体局部或全身中毒,造成呼吸系统损伤,呼吸功能衰竭,中毒严重者会在几分钟内缺氧窒息而死亡.现场急救及时给伤员采取应急供氧和防毒隔离等医疗措施,保持患者呼吸道畅通,防止呼吸功能衰竭发生,对提高救治率十分重要.但目前的急救器械不具有能同时供氧和防毒隔离等功能的医疗装备,这也影响了现场救治率的提高.

支原体肺炎(mycoplasma pneumoniae pnenmonia,MPP)是由肺炎支原体(Mycoplasma pneumoniae,MP)引起的肺炎.MPP起病缓,有发热、阵发性刺激性咳嗽,少量黏液性或黏液脓性痰.MPP多发于儿童或青少年,占肺炎总数的15％～30％[1].大多数MPP患儿血液中单核细胞百分比高于正常值(3.5％～7.9％),可达65.3％,这是否提示具有肺炎症状且伴有单核细胞增多的患儿为MP感染.为此,我们对2000-2011年住院治疗的MPP患儿的临床资料进行分析,报告如下.

Screening E-epitope of Japanese encephalitis virus （JEV）, creating the conditions for JEV prevention and treatment research by mimotope,providing important clues and basis for establishing specific serological diagnostic methods and exploring peptide vaccine of JEV. With the monoclonal antibody molecules of JEV E protein as solid phase screening, screening the phage heptapeptide library by a rule of reduction, combined, elution, amplification. Picked monoclonal phages and detect them by ELISA, the positive clones were sequenced. Designed and synthesised a cDNA sequence with 15 peptides （GGADSMSMAGMAVSY） and conducted a recombinant expression vector with pGEX-KG,induced to express the recombinant polypeptide and dectected it by Western blot. Phages were highly en- riched after four rounds of screening, it is proved that there were 22 positive clones by ELISA. The Western blot show that the recombinant polypeptide can bind to rabbit polyelonal anti-JEV antibody specifically. We identified the structure of JEV E protein-specific phage mimotope successfully,it laid a good foundation for luther study.

Objective:To investigate the drug sensitivity of mycoplasma pneumoniae isolated from patients.Methods:Samples of throat swab were cultivated in selective culture medium,and then they were detected by PCR.Mycoplasma pneumoniae were mixed with different quantity of antibiotics.If one drug could kill the microorganism,the color of the media would not change.Results: One clinical mycoplasma pneumoniae type was isolated successfully.When the concentration of azithromycin,erythromycin and cefuroxime sodium were diluted to 31 μg/mL,125 μg/mL,500 μg/mL,respectively,mycoplasma pneumoniae were supressed.Ceftriaxone sodium,mezlocillin and ampicillin had no effect on the growth of mycoplasma pneumoniae.Conclusions: The growth of mycoplasma pneumoniae isolated from throat swab samples is inhibited easily by azithromycin.Erythromycin and cefuroxime sodium also had inhibitory effect on mycoplasma pneumoniae.

加强军营内呼吸道传染病的监测与监控力度,对于指导防控该类传染病,保障部队战斗力具有重要的意义.为此,我们对北京部队2005-2008年呼吸道传染病的流行动态进行了回顾性分析,结果报告如下.

We identified the critical amino-acid residues in antigen M derterminant (MAD) epitope of human cytomegalovirus protein M. On the basis of the peptide sequence of MAD, some conservative residues were mutated into the glycine residue. Then the gene fragment of mutants linked to amino terminal of Fc were cloned into the plasmid pET32-Fc and expressed by fusion with Fc. After purified by protein A affinity chromatography, the activity of mutants binding the goat polyclonal antibodies against human cytomegalovirus (HCMV) were detected by ELISA and Western blotting. Our results showed that when glutamine residue was mutated into glycine residue, the activity of MAD(Q --> G) binding the goat polyclonal antibodies against HCMV was reduced apparently. Other mutants did not have the same characteristics. The activity of MAD was closely related to the conformation of glutamine residue.

长期、连续的疫情监测是各级疾病预防控制机构掌握传染病流行趋势及规律,指导传染病防治的重要手段[1],而采用有效的监测模式则是保证疾病监测质量的基础和前提.为了提高疫情监测工作质量,北京军区疾病预防控制中心在分析目前军队疫情监测网络直报系统应用现状的基础上,建立了多途径互补监控传染病疫情监测模式,即充分利用军队现有卫生信息系统.将多种疫情获取方式相结合,通过不同信息系统间数据的补充、完善,以便及时准确完整地收集军队传染病信息;同时由于系统间数据的相互监督作用.可有效提高疾病监测管理的质量.该模式在我军区应用已取得了初步成效,现报告如下.

Objective:To clone and express the epitope AD1 and AD2 of HCMV glycoprotein B(gB) in Escherichia coli,to purify the expression product and to detect sera from HCMV positive patients by ELISA assay for further clinical study.Methods:AD1 and AD2 genes were cloned by PCR using HCMV genome DNA as template and the recombinant expression vectors pET32-NusA-AD1 and pET32-NusA-AD2 were constructed.E.coli BL21(DE3)pLysS bearing the plasmids was induced with IPTG for protein production.The proteins NusA-AD1 and NusA-AD2 were purified by Ni2+ affinity chromatography and used to detect HCMV positive human sera by ELISA method.Results: The recombinant antigens NusA-AD1 and NusA-AD2 were expressed solubly.Of eight HCMV-IgG positive serum samples,five were found positive by NusA-AD1,and six were positive by NusA-AD2.Conclusion: Most HCMV positive human sera could be detected by recombinant antigens NusA-AD1 and NusA-AD2.