Craniomaxillofacial bone marrow mesenchymal stromal cells (BMSCs) retaining neural crest-derived neurogenic niche is driven by lineage memory and niche homeostasis. Elucidating how the neurogenic potential is maintained is critical for neurological health. Here, we explored a neural crest-like progenitor niche in BMSCs with high neurogenic and proliferative capacity by single-cell transcriptomics. In which, ANKRD1 is a pivotal regulator sustaining the neurogenic reservoir. Importantly, ANKRD1 expression in this niche declines with aging and lineage commitment, coinciding with its redistribution from a diffuse nucleoplasmic pattern to perinuclear enrichment along the nuclear lamina and loss of neural potential. Mechanistically, ANKRD1 preserves neurogenic capacity by directly binding super-enhancers of neural marker genes (SOX2, NESTIN) and maintaining open chromatin architecture. Critically, neuron-targeted ANKRD1 delivery rescues spatial memory deficits in aged mice. These findings establish ANKRD1 as a therapeutically tractable regulator that sustains neurogenic chromatin reservoirs to support neurocognitive resilience, opening avenues to counter cognitive aging.

Ribosomal RNA (rRNA) synthesis is intricately tied to cellular growth and proliferation. Basic fibroblast growth factor (FGF2), a pivotal factor for bone marrow mesenchymal stem cells (BMSCs), can stimulates rRNA transcription, though the underlying mechanism remains unknown. Here, we demonstrate that the cytoplasm-nucleus translocation of FGF2 is determined by the stable nuclear localization motif. Meanwhile, the nuclear FGF2 regulates rRNA expression and BMSCs proliferation via phase separation. Next, through FGF2 related epigenomics and 3D genomes analysis, we identified chromatin architectures during BMSCs differentiation and aging. In the process, topologically associating domains (TADs) and chromatin loops profiling revealed the attenuated genomic interaction among proximal chromosomes 13, 14, 15, 21, and 22, where phase-separated FGF2 facilitates rDNA transcription depend on specific super-enhancers (SEs). Furthermore, we validated that FGF2 orchestrates rDNA chromatin architecture in coordination with STAT5. Together, these findings underscore the pivotal role of FGF2 in rDNA chromatin architectures, which determines BMSCs cell fate.

The structure and topography of the scaffold material play an essential role in the skin wound healing process, and aligned nanofibers can guide cell growth and proliferation to some extent. It has been demonstrated that the optimal scaffold fabrication method for wound healing is to generate three-dimensional scaffolds composed of nanoscale fibers. Inspired by the structure and function of skin, composite aligned electrospun dressings with fiber gradient changes are prepared by continuous spinning through changing the relevant parameters of the electrospinning process, thereby creating a wettability gradient change to mimic the skin's hydrophilic-hydrophobic transition. This design efficiently manages exudates from the wound site and maintains humidity balance. The hydrophilic inner layer (A100) absorbs wound exudates and maintains a moist environment in the wound. The A300 layer, with its appropriate porosity, ensures nutrient and gas exchange at the wound site, providing an ideal microenvironment for cell growth. The outermost layer, A500, is a mechanical barrier to prevent bacterial invasion, wound infection, and over-hydration. In vitro experiments have verified that the dressing exhibits good biocompatibility and mechanical properties, while in vivo experiments have confirmed that the dressing promotes wound healing, collagen deposition, angiogenesis, hair follicle regeneration, and reduces fibrosis at the wound site. Additionally, it improves scar formation to a certain extent.

Repairing large segmental bone defects poses significant challenges in reconstructive surgery, due to the need for scaffolds that balance robust mechanical properties with effective bone regeneration performance. Hierarchically structured scaffolds designed to mimic the native bone microenvironment have shown significant potential but often fail to fully address this balance. In this study, we propose an approach by integrating autogenous lyophilized platelet-rich fibrin (L-PRF)-a rich source of platelets and growth factors-with a 3D-printed (3-tricalcium phosphate ((3-TCP) ceramic framework to manufacture a hierarchically structured bioactive L-PRF/ (3-TCP composite scaffold. The (3-TCP ceramic framework ensures robust mechanical support, while the L-PRF offers an extracellular matrix (ECM)-like bioactive microenvironment that enhances cellular responses and enables a sustained release of autologous bioactive factors. Comprehensive in vitro and in vivo assessments demonstrate the superior efficacy of the scaffold in promoting bone regeneration, positioning it as a highly promising bioactive material for large segmental bone defect repair in bone tissue engineering.

PURPOSE:To evaluate the effet of one-time one-abutment placement on peri-implant tissues.METHODS:Thirty-five patients with single posterior loss were collected,who received definitive abutment at the moment of implant placement.One day and after 1 year of implant loading,radiographic assessment of marginal bone level changes and clinical status of peri-implant soft tissues were conducted.Plaque index,pocket depth as well as sulcus bleeding index were assessed.RESULTS:During 1 year follow-up period after loading,no implant failure was observed.The mean marginal bone loss of implants were(0.225±0.1 13)mm mesially and(0.439±0.123)mm distally.Standard periodontal probes were used to measure plaque index,probing depth,and gingival crevicular bleeding index immediately after repair and 1 year later.CONCLUSIONS:In the posterior region,one-time one-abutment placement may better protect peri-implant tissues as an ideal treatment protocol.

OBJECTIVE:The aim of this population-based retrospective study was to compare the osteogenic effect of newly formed bone after maxillary sinus floor elevation (MSFE) and simultaneous implantation with or without bone grafts by quantitatively analyzing trabecular bone parameters. METHODOLOGY:A total of 100 patients with missing posterior maxillary teeth who required MSFE and implantation were included in this study. Patients were divided into two groups: the non-graft group (n=50) and the graft group (n=50). Radiographic parameters were measured using cone beam computed tomography (CBCT), and the quality of newly formed bone was analyzed by assessing trabecular bone parameters using CTAn (CTAnalyzer, SkyScan, Antwerp, Belgium) software. RESULTS:In the selected regions of interest, the non-graft group showed greater bone volume/total volume (BV/TV), bone surface/total volume (BS/TV), trabecular number (Tb. N), and trabecular thickness (Tb. Th) than the graft group (p<0.001). The non-graft group showed lower trabecular separation (Tb. Sp) than the graft group (p<0.001). The incidence of perforation and bleeding was higher in the graft group than in the non-graft group (p<0.001), but infection did not significantly differ between groups (p>0.05). Compared to the graft group, the non-graft group showed lower postoperative bone height, gained bone height and apical bone height (p<0.001). CONCLUSION:MSFE with and without bone grafts can significantly improve bone formation. In MSFE, the use of bone grafts hinders the formation of good quality bone, whereas the absence of bone grafts can generate good bone quality and limited bone mass.

Nanoparticles-based glues have recently been shown with substantial potential for hydrogel adhesion. Nevertheless, the transformative advance in hydrogel-based application places great challenges on the rapidity, robustness, and universality of achieving hydrogel adhesion, which are rarely accommodated by existing nanoparticles-based glues. Herein, we design a type of nanohesives based on the modulation of hydrogel mechanics and the surface chemical activation of nanoparticles. The nanohesives can form robust hydrogel adhesion in seconds, to the surface of arbitrary engineering solids and biological tissues without any surface pre-treatments. A representative application of hydrogel machine demonstrates the tough and compliant adhesion between dynamic tissues and sensors via nanohesives, guaranteeing accurate and stable blood flow monitoring in vivo. Combined with their biocompatibility and inherent antimicrobial properties, the nanohesives provide a promising strategy in the field of hydrogel based engineering.

目的 探讨不同种植位点联合翼上颌种植对上颌无牙颌种植体与骨组织生物力学影响,为临床选择符合生物力学原则的种植设计方案提供依据.方法 选取1例上颌后牙骨量不足的无牙颌锥形束CT扫描数据,完成上颌骨三维实体模型的建立,利用冠修复体和种植体数据,分别建立冠修复体和种植体-基台一体三维实体模型,设计5组不同种植位点的三维有限元模型,以单侧200 N的垂直载荷和100 N斜向载荷分别在双侧后牙区加载,应用ANSYS有限元分析软件计算种植体和周围骨组织表面的应力分布,采用SPSS 26.0软件包对数据进行统计学分析.结果 ①5组模型均显示最大应力集中在种植体颈部和颈部皮质骨处.②5组模型在斜向载荷下的种植体最大应力值大于垂直载荷(P<0.05),骨组织周围最大应力值差异无统计学意义.③不同种植位点联合翼上颌种植模型间种植体及骨组织最大应力值比较,差异无统计学意义.结论 上颌无牙颌种植固定修复,双侧对称性植入翼上颌种植体,改变前部种植体位点不会影响整个设计的应力分布.

目的 探讨种植时机对老年合并牙周感染患者种植治疗的影响.方法 86 例老年合并牙周感染患者,按照随机数字、双盲的原则均分为两组,对照组在拔牙后予以延期种植,观察组拔牙后予以即刻种植.对比分析治疗后种植体留存率、探诊深度(PD)、牙龈出血指数(BI)、红色美学量表(PES)等情况.结果 两组种植成功率及种植体留存率无统计学差异(P>0.05),术前两组PD差异无统计学意义(P>0.05),观察组术后3、6、12 个月PD显著高于对照组(P<0.01);两组种植体松动度差异无统计学意义(P>0.05),观察组修复时BI指数显著高于对照组(P<0.05),观察组术后 3 个月PES评分显著高于对照组(P<0.05),两组术后 6、12 个月PES评分差异不显著(P>0.05);观察组在生活质量评分方面,生理疼痛、社交障碍、功能障碍、身心障碍评分显著低于对照组(P<0.05).结论 老年合并牙周感染患者拔牙后即刻种植与延期种植均可取得良好的临床效果,即刻种植患者虽然会表现出较大的探诊深度和牙龈出血量,但可以缩短治疗时间,提高生活质量,因此需针对患者情况进行个性化选择.

Background The purpose of this study was to investigate the socket healing outcome after alveolar ridge preservation at infected molar sites using an erbium-doped yttrium aluminium garnet (Er:YAG) laser. Methods Eighteen patients who needed molar extraction and exhibited signs of infection were included and allocated into either the laser group or the control group. Er:YAG laser irradiation for degranulation and disinfection was performed with alveolar ridge preservation (ARP) in the laser group. Traditional debridement with a curette was performed in the control group. Two months after ARP, bone tissue samples were harvested at the time of implant placement for histological analysis. Assessment of dimension changes in alveolar bone was conducted by superimposing two cone-beam computed tomography (CBCT) scans taken at baseline and two months after extraction. Results Histologically, after two months of healing, Er:YAG laser treatment resulted in more newly formed bone (laser: 17.75 ± 8.75, control: 12.52 ± 4.99, p = 0.232). Moreover, greater osteocalcin (OCN) positive expression and lower runt-related transcription factor 2 (RUNX-2) positive expression were detected in the laser group. However, no statistically significant difference was observed between the two groups. The difference in the vertical resorption of the buccal bone plate was statistically significant between groups (laser: -0.31 ± 0.26 mm, control: -0.97 ± 0.32 mm, p < 0.05). Major changes in ridge width were observed at 1 mm below the bone crest. However, the differences between groups were not significant (laser: -0.36 ± 0.31 mm, control: -1.14 ± 1.24 mm, p = 0.171). Conclusions ARP with Er:YAG laser irradiation seemed to improve bone healing by regulating osteogenesis-related factor expression in the early stage at infected sites. Trial registration The trial was registered on the Chinese Clinical Trial Registry Platform ( https://www.chictr.org.cn/ ) (registration number: ChiCTR2300068671; registration date: 27/02/2023).

临床收治的全身性骨代谢疾病以骨质疏松症较为常见,患病群体主要集中于中老年群体.随着中国人口老龄化趋势的不断加剧,该病患病率也随之升高.骨质疏松症已被证实为牙种植治疗的高风险因素,可能引发种植体初期稳定性不足、骨结合不良或者病理性边缘骨吸收等问题,使得种植治疗远期效果欠佳,甚至降低患者的治疗安全性.该文就骨质疏松症对种植治疗的影响及该类患者的治疗进展情况进行综述.

Uniform and porous chitosan-based microparticles with injectable and shape-memory properties are particularlyattractive due to their promising application potential for tissue engineering and regenerative medicine. However, simple andefficient methods for producing this kind of microparticle are still desirable. In this study, we report that uniform, injectable, andshape-memory chitosan microsponges were efficiently prepared by combining microfluidic emulsion with further freezing and in situthawing processes without using any potentially cytotoxic chemical cross-linker. The produced chitosan microsponges havecontrollable size and could be easily injected with syringe needles. Structural observations confirmed that the chitosan microspongehad an interconnected porous structure with pore size of several micrometers and could withstand a large compressive strain of similar to 93% and then recover similar to 96% of its initial diameter without structural damage. The chitosan microsponges showed a high porosity(similar to 84%) and swelling ratio (similar to 3800%) as well as good antibacterial activity. Additionally, an in vitro cell coculturing investigationrevealed that they also had good biocompatibility and exhibited great superiority to support cell adhesion and proliferation in threedimensions. The kind of chitosan microsponge presented here has great potential to serve as cell carriers for biomedical applications, especially as injectable scaffolds for regeneration and reconstruction of tissue defects

Background : Regenerative medicine by using stem cells from dental pulp is promising for treating patients with critical limb ischemic (CLI). Here, we investigated the difference in the angiogenetic ability of stem cells from human exfoliated deciduous teeth (SHED) and human dental pulp stem cells (DPSC). Methods : SHED and DPSC were harvested from dental pulp and analyzed in flow- cytometry for detecting the expression of surface markers. Levels of angiogenetic marker were examined by RT-PCR and Western-blot. Eighteen immunodeficient mice of critical limb ischemic model were divided into three groups: SHED, DPSC and saline, which was administered with SHED, DPSC or saline intramuscularly. Histological examination was performed to detect the regenerative results. Results : A highly expression of CD146 was detected in SHED. Moreover, cells with negative expression of both CD146 and CD31 in SHED were more in comparison with those in DPSC. Expression of angiogenesis factors including CXCL12, CXCR4, Hif-1a, CD31, VEGF and bFGF were significant higher in SHED than DPSC by the RT-PCR and Western-Blot results. SHED induced more CD31 expression and less fibrous tissue formation in the critical limb ischemic model as compare with DPSC and saline. Conclusion : Both SHED and DPSC possessed the ability of repairing CLI. With expressing more proangiogenesis factors, SHED may have the advantage of repairing CLI.

目的 分析种植体失败的影响因素,以期为临床工作提供理论依据.方法 收集2012年1月至2021年1月于安徽医科大学附属口腔医院种植中心接受种植治疗且病历资料完整的病例,选出种植体失败的病例,以种植体为单位,分析性别、年龄、牙周病史、手术部位、外科手术及种植体相关因素对种植体失败的影响.采用统计学软件SPSS 24.0,经卡方检验及Fisher精确检验筛选出有统计学意义的变量,纳入二元Logistic回归分析,P<0.05表示统计学有显著性差异.结果 9年间共植入6124颗种植体,其中失败种植体共计82颗,种植体失败率为1.34％.早期种植体失败72颗,晚期种植体失败10颗,失败率分别为1.18％和0.16％.统计分析显示,男性比女性种植体失败的可能性高(P<0.001),种植体直径<4.0 mm、种植体长度<10 mm、Ⅳ类骨质种植体失败率高(P<0.01),行穿牙槽嵴上颌窦底提升(transalveolar technique for sinus floor elevation,TTSF)比不行TTSF手术种植体失败的可能性高(P<0.001),未行大颗粒喷砂酸蚀(sandblasted large-grit acid-etched,SLA)表面处理种植体比SLA(含SLActive)表面处理种植体失败的可能性高(P=0.001);而年龄、上下颌骨、手术部位、种植体形状(是否为锥形)、是否行侧壁开窗上颌窦底提升(lateral approach of sinus floor elevation,LASF)及引导骨再生(guided bone regeneration,GBR)对种植体失败影响无统计学意义.结论 与种植体失败有关的影响因素包括男性、Ⅳ类骨、种植体直径<4 mm、种植体长度<10 mm、非SLA表面处理种植体、行TTSF及牙周病史.

An ideal bone repair scaffold is expected to possess superior architectural characteristics to facilitate the adhesion, proliferation, and migration of bone-repair-related cells, while excluding nonosteogenic cells and fibrous tissues from interfering with normal bone regeneration. Unfortunately, such scaffold material has rarely been reported. Herein, nanocomposite scaffolds with a radially ordered porous structure are presented, manufactured using a modified directional freeze-casting method, and are promising bone defect repair materials to satisfy this requirement. The prepared nanocomposite scaffolds consist of a natural bio-macromolecule, chitosan, and bioactive hydroxyapatite nanoparticles derived from porcine cortical bone, demonstrating favorable biocompatibility and biological functions. Both in vitro cell studies and in vivo animal studies reveal the great superiority of the radially oriented porous structure of the scaffolds in guiding bone regeneration, while simultaneously preventing the invasion of surrounding nonosteogenic cells and fibrous tissue, compared to the axially oriented porous structure. This work indicates the distinctive potential of radially oriented porous scaffolds for repairing tabular and lacunar bone defects.

报道1例曾接受双肾移植,因右下颌磨牙缺失导致咀嚼功能受限,通过种植治疗恢复缺失牙的患者.经过完善的术前检查制定治疗计划,术中严格执行无菌操作,术前、术后预防感染.治疗结束后患者缺失牙的功能和美观均得以恢复,随访2年,修复体行使功能良好,种植体周围骨吸收稳定,但远期效果有待进一步观察.

Introduction Micro-sized sponge particulates have attracted extensive attention because of their potential to overcome the intrinsic limitations of conventional monolithic scaffolds in tissue engineering. Bioactive nanocomposite microsponges are regarded as potential bone substitute materials for bone regeneration. Methods Based on a combination of microfluidic emulsion with further freezing and in situ thawing, chitosan (CS)-hydroxyapatite (HAP) microsponges were prepared and characterized in terms of their morphology and elemental distribution using a scanning electron microscope equipped with an X-ray detector. The swelling ratio, porosity, degradability, antibacterial activity, and bioactivity were detected and analyzed. The biological functions of the CS-HAP microsponges were examined to assess the adhesion, proliferation, and differentiation of in vitro co-cultured rat bone marrow mesenchymal stem cells (rBMSCs). Furthermore, the CS-HAP microsponges were used as cell-free scaffolds and implanted into calvarial defects in a rat model to evaluate the in vivo osteogenesis. Results The CS-HAP microsponges have a porous structure with high porosity (~76%), good swelling capacity (~1900%), and shape-memory properties. The results of in vitro experiments show that the CS-HAP microsponges achieve good bioactivity and promote osteogenic differentiation of rBMSCs. Furthermore, the CS-HAP microsponges significantly promote bone regeneration in rat calvarial defects. Conclusion The bioactive CS-HAP microsponges have the potential to be used as bone substitute materials for bone tissue engineering.

Background: The role of sex hormones and their receptors has drawn much attention in the process of cartilage regeneration. This study aimed to investigate the effect of androgen receptor (AR) on the chondrogenic ability of articular chondrocytes and the related mechanism. Methods: Articular chondrocytes were isolated, cultured, identified by toluidine blue staining and then transduced with lentivirus carrying the AR gene. The cell viability was determined using Cell Counting Kit-8, and cell apoptosis was assessed by flow cytometry analysis. The effects of AR overexpression on the expression of cartilage-specific proteins and some signalling molecules were evaluated by real-time PCR and Western blotting. Using 24 New Zealand rabbits, the regeneration of rabbit articular cartilage defects was further investigated in vivo and evaluated histologically. Results: The overexpression of AR significantly reduced the apoptosis rate of chondrocytes but did not affect their proliferation. The overexpression of AR also promoted the expression of Sry-related HMG box 9, collagen II and aggrecan, decreased the expression of matrix metalloproteinase-13, and downregulated p-S6 and RICTOR. The experimental group with AR-overexpressing chondrocytes exhibited superior regeneration of cartilage defects. Conclusion: AR overexpression can maintain the phenotype of chondrocytes and promote chondrogenesis in vitro and in vivo . mTOR-related signalling was inhibited.

目的 研究新型光敏剂NHS-BODIPY-Br在体外对牙龈卟啉单胞菌(P gingivalis)和福赛坦菌(T forsythia)生物膜的抗菌作用.方法 CCK-8检测光敏剂NHS-BODIPY-Br的生物相容性,将P gingivalis和T forsythia在厌氧(10％CO2,10％H2,80％N2)条件下混合培养48 h形成生物膜后进行抗菌光动力治疗,光敏剂浓度分别为5、10、15 mg/L.激光强度分别为15和30 J/cm2.治疗后进行细菌活力检测、残留脂多糖检测以及激光共聚焦分析,并确定最佳治疗组合.结果 当光敏剂NHS-BODIPY-Br的浓度为10 mg/L,光强度为15 J/cm2时细菌生物膜减少率约98％.结论 该研究说明用光敏剂NHS-BODIPY-Br进行抗菌光动力治疗可以有效地抑制P gingivalis和T forsythia生物膜.