Background Osteosarcoma (OS) is one of the most common malignancies arising in bone. Hypoxia and immune regulation are pivotal in tumor biology. However, their combined effects and mechanisms in OS remain understudied. This study aimed to explore the role and mechanism of hypoxic-induced M2 macrophages in promoting the progression of OS. Methods Differentially expressed proteins in hypoxic macrophage supernatants were detected by antibody array. Cell functional experiments, siRNA-mediated gene silencing, and overexpression transfection were used to study osteopontin (OPN) and its supernatant effect. Bioinformatics analysis was applied to investigate downstream targets and pathways, and a xenograft model was established to assess in vivo effects. Results Our data revealed that hypoxic M2 macrophage supernatant enhanced OS malignancy and epithelialmesenchymal transition, activating cancer pathways. Hypoxia upregulated OPN in M2 macrophages, and OPN inhibition reduced its tumor-promoting effect. Early growth response 3 (EGR3) was differentially expressed in OS cells treated with the supernatant, and its overexpression inhibited OS cell migration, reversing tumor promotion. Interferon-stimulated gene 15 (ISG15), a key differentially expressed gene related to OPN and EGR3 overexpression, inhibited OS cell proliferation and migration. Additionally, OPN increased retinoic acid-inducible gene I (RIG-I) expression and enhanced signal transducer and activator of transcription 3, nuclear factor kappa B, and extracellular signal-regulated kinase signaling, while EGR3 and ISG15 overexpression inhibited these effects. Silencing ISG15 restored pathway activation and reversed the inhibitory effect of EGR3 on OS cell migration. Dual-Luciferase reporter gene assay confirmed that EGR3 activates ISG15 transcription. OPN treatment upregulated DNA (cytosine-5)methyltransferase 1 (DNMT1) expression, and ChIP assays demonstrated that EGR3 overexpression enhanced DNMT1 binding to the EGR3 promoter. These findings suggest that OPN promotes OS malignancy by downregulating EGR3 and ISG15, and by enhancing RIG-I expression, as validated in a xenograft model of OS. Conclusion Our findings demonstrate that hypoxic-induced M2 macrophages promote OS progression through OPN-dependent mechanisms, including inhibition of EGR3 and ISG15 expression and upregulation of RIG-I.

BackgroundCancer-associated fibroblasts (CAFs) contribute to the progression and treatment of breast cancer (BRCA); however, risk signatures and molecular targets based on CAFs are limited. This study aims to identify novel CAF-related biomarkers to develop a risk signature for predicting the prognosis and therapeutic response of patients with BRCA.MethodsCAF-related genes (CAFRGs) and a risk signature based on these genes were comprehensively analyzed using publicly available bulk and single-cell transcriptomic datasets. Modular genes identified from bulk sequencing data were intersected with CAF marker genes identified from single-cell analysis to obtain reliable CAFRGs. Signature CAFRGs were screened via Cox regression and least absolute shrinkage and selection operator (LASSO) analyses. Multiple patient cohorts were used to validate the prognosis and therapeutic responsiveness of high-risk patients stratified based on the CAFRG-based signature. In addition, the relationship between the CAFRG-based signature and clinicopathological factors, tumor immune landscape, functional pathways, chemotherapy sensitivity and immunotherapy sensitivity was examined. External datasets were used and sample experiments were performed to examine the expression pattern of MFAP4, a key CAFRG, in BRCA.ResultsIntegrated analyses of single-cell and bulk transcriptomic data as well as prognostic screening revealed a total of 43 prognostic CAFRGs; of which, 14 genes (TLN2, SGCE, SDC1, SAV1, RUNX1, PDLIM4, OSMR, NT5E, MFAP4, IGFBP6, CTSO, COL12A1, CCDC8 and C1S) were identified as signature CAFRGs. The CAFRG-based risk signature exhibited favorable efficiency and accuracy in predicting survival outcomes and clinicopathological progression in multiple BRCA cohorts. Functional enrichment analysis suggested the involvement of the immune system, and the immune infiltration landscape significantly differed between the risk groups. Patients with high CAF-related risk scores (CAFRSs) exhibited tumor immunosuppression, enhanced cancer hallmarks and hyposensitivity to chemotherapy and immunotherapy. Five compounds were identified as promising therapeutic agents for high-CAFRS BRCA. External datasets and sample experiments validated the downregulation of MFAP4 and its strong correlation with CAFs in BRCA.ConclusionsA novel CAF-derived gene signature with favorable predictive performance was developed in this study. This signature may be used to assess prognosis and guide individualized treatment for patients with BRCA.

目前我国医学院校对培育学生医学人文素养重视不足,在当前患者维权意识提高及经济大潮对医疗界的冲击下,医学生走上临床可能会面临紧张的医患关系局面,影响并干扰诊疗的全程.笔者在乳腺外科医学生线上教学过程中通过课前习题答卷分析医学生人文素养现状,结合其对临床工作的意义,在将人文课程融入并贯穿专业课程、引入专业师资力量改革教学及考核方式、以实践促进医学人文素养的提升等方面提出对策,以期为提升医学生的医学人文素养提供可行思路.

Abstract BACKGROUND: Traditional Chinese Medicine (TCM) has consistently demonstrated promise in the prevention and management of ONFH. Epimedium, historically revered in Chinese medicinal recipes, has been utilized for mitigating conditions such as osteonecrosis and symptoms of kidney yang deficiency. OBJECTIVE: This study aimed to forecast the drug targets and associated pathways through which Epimedium exerts its therapeutic effects against osteonecrosis of the femoral head. Additionally, we sought to delve deeper into its mechanism at the molecular level. METHODS: In this study, we identified the active constituents and targets of Epimedium using the TCMSP database. The GEO database (with accession number GSE123568) was consulted to pinpoint targets associated with SONFH. Differential gene expression was visually represented through volcano and heat maps, crafted using the R software. GO and KEGG analyzes of these target genes were also subsequently performed using R software. RESULTS: Five pivotal target genes were identified: PTGS2, KCNH2, BCL2L1, ABCG2, and E2F2. An exhaustive topological analysis was performed encompassing eight pathways and three genes. CONCLUSION: This study elucidates the fundamental constituents, specific targets, and molecular pathways that underlie the effectiveness of Epimedium in the treatment of osteonecrosis of the femoral head.

根据平战时舰艇伤病员特点,针对舰艇医务人员专业能力不足、卫生装备难以满足海上救治需求现状,提出了应在明确舰艇医务室救治范围的同时从系统建立舰艇医务人员培训机制、重点强化现场救治科目培训、针对性地配套研发卫生装备3个方面,加强舰艇医务人员继续教育培训和舰艇医务室软硬件建设,提升舰艇医务室初级救治能力,胜任"铂金10分钟"和"黄金1小时"的紧急救治,更好地保障和形成战斗力.

患者女，71岁，因“右侧乳房皮肤红色斑块15年，加重1年”入院。查体：右侧乳房内侧及胸正中的皮肤有红色湿疹样改变，中央有带缝线切口（图1），同侧腋窝未触及肿大淋巴结。病理活检提示：（乳房区皮肤）Paget病可能。免疫组化检查示：CK7（+），CK8（+），CK10/13（-），CEA（部分+），S100（-），ER（部分+），PR（-），CK20（-），AR（+），Her-2（+++），Ki67（30%~60%+）。超声检查提示：乳腺腺体内未见明显占位性回声。乳腺增强MR表现：未见明确异常强化占位灶。行右乳皮肤病损切除术+腹部大V形皮瓣转移术。冰冻病理检查示：（右侧乳房）皮肤Paget病，上、下、内、外切缘及基底切缘（-）。术后病理报告：带皮肤组织的右侧乳腺，灰黄、灰红色乳腺组织1块，大小9 cm×8 cm×3.2 cm，皮肤大小8 cm×8 cm，切面距上切缘3.2 cm、下切缘4 cm、内切缘3 cm、外切缘1.5 cm处见1个皮肤粗糙区，大小3.5 cm×1.3 cm，灰白色，实性，质硬，界不清。光镜检查：右侧乳腺组织见肿瘤位于表皮内，肿瘤细胞大、圆形，胞质丰富，核大，异型明显，呈小簇状、团巢状或散在分布于皮肤表皮内（图2）。未见浸润乳腺实质，未见导管内癌成分。上、下、内、外切缘及基底切缘均（-）。诊断为（右侧乳房）皮肤Paget病。随访2个月，局部未见复发，无远处转移，无并发症。

目的 评估不同环境下的海军军人自身免疫性甲状腺疾病的患病情况,以便前移防控措施.方法 回顾性分析2020年6月至9月在海军军医大学(第二军医大学)第一附属医院体检中心进行健康体检的2198例海军官兵体检数据.按驻地不同,将官兵分为城市组(驻扎在沿海城市的官兵)和舰船组(驻扎在舰船的官兵),比较两组海军官兵的血清三碘甲腺原氨酸(T3)、甲状腺素(T4)、促甲状腺激素(TSH)、甲状腺过氧化物酶抗体(TPOAb)、甲状腺球蛋白抗体(TgAb)水平及甲状腺超声结果,并采用多因素logistic回归分析探讨甲状腺功能、甲状腺抗体水平及甲状腺超声结果的影响因素.结果 城市组1095例,年龄为34(29,41)岁,男663例(60.55％)、女432例(39.45％);舰船组1103例,年龄为33(28,38)岁,男1014例(91.93％)、女89例(8.07％).两组的性别及年龄差异均有统计学意义(P均<0.01).城市组甲状腺抗体水平异常111例(10.14％,111/1095),亚临床甲状腺功能亢进(简称"亚甲亢")9例(0.82％,9/1095),亚临床甲状腺功能减退(简称"亚甲减")85例(7.76％,85/1095),超声检查有甲状腺结节363例(45.72％,363/794);舰船组甲状腺抗体水平异常84例(7.62％,84/1103),亚甲亢5例(0.45％,5/1103),亚甲减38例(3.45％,38/1103),超声检查有甲状腺结节258例(33.64％,258/767).多因素logistic回归分析结果显示,性别与甲状腺抗体水平异常有关(P<0.01),驻地和甲状腺抗体水平是TSH的影响因素(P均<0.01),性别、年龄及甲状腺抗体水平与甲状腺结节的发生有关(P均<0.01).结论 驻地在沿海城市的海军官兵甲状腺抗体水平异常率及亚甲减、甲状腺结节患病率较高,有必要对其给予更多关注.女性海军官兵更需前移防控措施,而且甲状腺抗体水平升高的女性海军官兵随年龄的增大需对结节的良恶性进一步评估.

Objective:There is currently no consensus on the ideal plane for implant placement in breast reconstruction. This meta-analysis provides a comparison of prepectoral breast reconstruction (PBR) vs. subpectoral breast reconstruction (SBR), with primary outcomes of surgical efficacy and patient safety, to find out the best surgical approach.Methods:PubMed, Cochrane, Web of Science, and Embase databases were searched from January 1st, 2019, to April 1st, 2022, to retrieve studies that compared PBR with SBR after mastectomies. The main outcomes were surgical complications and satisfaction with breasts domain. The literature was screened according to the inclusion and exclusion criteria, the data was extracted and the methodological quality of the included studies was assessed by 2 reviewers independently. Meta-analysis was performed using RevMan 5.3.Results:A total of 14 comparative studies with 2 355 patients were included. The meta-analysis showed no statistical differences in seroma, implant removal, flap necrosis, capsular contracture, and wound dehiscence between PBR and SBR groups. PBR patients demonstrated lower hematoma, animation deformity rates, and higher BREAST-Q scores compared to SBR groups.Conclusions:Surgical efficacy and patient safety are similar between PBR and SBR groups. PBR patients have lower hematoma, animation deformity rates and are more satisfied with breasts domain. Further well-designed multi-center prospective studies are required to increase the robustness of the findings.

目的 建立亚临床甲状腺功能亢进和减退2种不同甲状腺功能状态的小鼠模型,并明确亚临床甲状腺功能水平不同对小鼠Morris水迷宫实验中学习记忆行为的影响.方法 选取30只Balb/C雄性小鼠,周龄5～6周,适应性饲养1周后,随机分为亚甲亢组、亚甲减组和对照组,每组10只.经高、低剂量摸索后确定造模用药剂量.观察3组小鼠对声、光等外界刺激的反应.3周后行Morris水迷宫实验,对比分析3组小鼠行为学差异.同时采集血清标本验证是否造模成功.结果 亚甲亢组小鼠每日给予腹腔注射优甲乐25μg/100 g鼠重,亚甲减组每日给予小鼠腹腔注射甲巯咪唑(MMI)1.5 mg/100g鼠重,对照组常规喂养.亚甲亢组促甲状腺激素(TSH)为(0.27±0.09)mU/L,亚甲减组TSH为(3.15±0.68)mU/L,对照组TSH为(1.01±0.16)mU/L,3组差异有统计学意义(P<0.01),三碘甲状腺原氨酸(T3)、甲状腺素(T4)差异无统计学意义(P>0.05).3组小鼠天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)水平差异无统计学意义(P>0.05).定位航行实验,与对照组相比,亚甲亢组每日逃避潜伏期明显缩短(P<0.05),分别为(68±29)s、(51±36)s、(41±39)s、(38±41)s,而亚甲减组差异无统计学意义(P>0.05).空间探索实验,亚甲亢组目标象限路程比为(29±16)％,亚甲减组为(14±11)％,对照组为(15±11)％,亚甲亢组目标象限时间比为(33±20)％,亚甲减组为(15±14)％,对照组为(17±12)％,亚甲亢组穿台次数为2.0(1.75,3.0)次,亚甲减组为1.0(0,1.0)次,对照组为1.0(0.75,1.0)次.亚甲亢组3组指标均显著高于对照组(P<0.05),而亚甲减组差异无统计学意义(P>0.05).3组平均速度差异无统计学意义(P>0.05).结论 运用优甲乐和甲巯咪唑腹腔注射的方法可成功建立亚甲亢和亚甲减小鼠动物模型.通过Morris水迷宫实验发现亚甲亢组小鼠的空间学习、记忆能力比对照组小鼠增强,而亚甲减组小鼠无明显差异.

医疗帮扶是国务院关于打赢脱贫攻坚战中扭转因病致贫的一项重要举措.为了深入贯彻落实《中共中央国务院关于打赢脱贫功坚战的决定》和《关于印发加强三级医院对口帮扶贫困县县级医院工作方案的通知》,海军军医大学附属长海医院于2017年成立了赴安徽省泗县人民医院(二级甲等)对口帮扶专家组,一行5人分别从事不同专业,在泗县人民医院开展每年3个月连续4年的对口帮扶及临床诊疗服务.笔者作为一名乳腺、甲状腺外科副教授,参加了2018年的医疗帮扶工作并担任组长,希望通过切实的临床工作和临床带教提升县医院普通外科对乳腺、甲状腺疾病的诊疗能力,为其开设亚专业——乳腺、甲状腺外科做铺垫.以下是医疗帮扶过程中的一些经验和体会.

乳腺真空辅助微创活检(VABB)在国内广泛用于乳腺良性病灶的切除,在诊断恶性肿瘤方面也安全、准确,非常方便.在乳腺影像报告和数据系统(BI-RADS)3级病损中,对于扁平上皮非典型病变、经典小叶肿瘤、乳头病变和经核心针活检或VABB诊断的放射状瘢痕建议采用VABB切除而非开放性手术.影像引导的VABB是微钙化安全的诊断方法,并且正在向完全性切除发展.高龄、活检病灶有残留、伴有不典型增生及外周型导管内乳头状瘤在VABB术后需格外注意.对以小癌灶为表现的早期乳腺癌,行VABB术后仍有一定的肿瘤残留率,目前确诊恶性的肿瘤均建议进一步扩大切除.采用VABB结合前哨淋巴结活检作为乳腺癌局部治疗措施的时代尚未来到,新辅助化疗后采用VABB评估也在探索中.本文就VABB处理可疑恶性病灶的研究进展作一综述.

目的 运用Meta分析系统评价微波消融和传统开放手术治疗甲状腺微小乳头状癌的疗效及安全性.方法 确定检索策略、文献的纳入与筛选原则,分别检索Pubmed(Medline)、Cochrane Library、中国生物医学文献数据库(Sinomed)、中国知网(CNKI)、万方数据库中关于微波消融治疗甲状腺微小乳头状癌的随机对照研究,实验组为微波消融法治疗,对照组为传统开放手术治疗.检索时间跨度为2017年4月至2020年4月.运用RevMan 5.3统计软件进行Meta分析.结果 纳入研究文献10篇,研究对象1307人,其中试验组669人,对照组638人.结果 显示微波消融组和传统开放手术组的手术情况、术后并发症发生率、甲状腺激素水平和炎症因子水平的差异均具有统计学意义.相比之下,微波消融在以上方面的优势均较明显,肿瘤体积变化也非常明显.结论 微波消融法在治疗微小甲状腺乳头状癌方面具有手术创伤小、恢复快、美观度高、手术并发症少等特点,但是该10项研究均为近3年研究,随访时间短,其远期效果还有待未来大样本、长期随访的RCT研究来证实.

目的 系统评价不同药物联合伽玛刀治疗非小细胞肺癌(NSCLC)术后患者的疗效与不良反应的差异,为临床用药提供循证参考.方法 计算机检索Cochrane图书馆、PubMed、中国知网数据库、万方数据库、中文科技期刊数据库,收集不同药物联合伽玛刀治疗(试验组)对比单纯伽玛刀治疗(对照组)用于NSCLC术后患者的随机对照试验(RCT).筛选文献,采用Stata 16.0软件、RevMan 5.3软件进行meta分析.结果 14篇RCT文献纳入研究,包含1059例患者(试验组485例,对照组574例),共涉及10种药物联合伽玛刀干预措施.meta分析结果显示:试验组的近期疗效优于对照组,差异有统计学意义[RR(95％CI):1.19(1.10～1.30),P<0.0001],其中,伽玛刀配合小牛血清去蛋白注射液、血府逐瘀汤加味的治疗的RR值最高,效果最好.试验组和对照组发生胃肠道反应和骨髓抑制反应病例数的差异均无统计学意义[R R(95％C I):0.79(0.46～1.35),P=0.39;R R(95％C I):0.89(0.59～1.36),P=0.60],其中伽玛刀配合中医养阴清肺解毒法辨证施治减轻胃肠道反应的效果显著,RR值最低;伽玛刀配合康莱特治疗的骨髓抑制反应较轻.结论 小牛血清去蛋白注射液、血府逐瘀汤加味联合伽玛刀治疗NSCLC术后患者较为安全有效,能够增强近期疗效;中医养阴清肺解毒法辨证施治、康莱特配合伽玛刀治疗方案可以较好地提高患者的生活质量,减少不良反应,具有临床应用价值.

乳头乳晕复合体(NAC)血供分为内、外、上、下及中央5个区域,内上区是主要血供来源,主要血管来源于胸廓内动脉、胸外侧动脉分支;双侧NAC血供96％对称,但乳房肥大者有近一半双侧不对称.在保留乳头的手术中,术后乳头缺血坏死是一种严重并发症,血管造影或增强MRI有助于术前评估乳头乳晕区血供.乳腺肿瘤整形术、乳房重建术以及缩乳成形等手术的顺利开展均有赖于对NAC血供的掌握.必须经乳晕切口时,优选下缘切口,可通过乳晕周围去表皮操作保留真皮下血供,弥补乳晕切开范围过大的不足.另外,还要充分评估吸烟、糖尿病、肥胖、高血压,以及以往手术瘢痕对乳头乳晕血供的影响.

甲状腺外科是外科学下的一个分支学科,外科手术是甲状腺疾病治疗尤其是恶性疾病治疗最有效的措施.现阶段新冠肺炎疫情防控要求网络教学为主,高等医科院校甲状腺外科学教学也需要探索新颖、高效的在线教学模式.“益心保甲”微信公众号作为甲状腺外科网络自主学习平台,汇聚往年实施翻转课堂中优秀课件及视频辅助教学.此模式对传统课堂教学是一种有效补充,尤其适用于疫情期的线上教学.

目的:探讨新冠肺炎疫情期间学生使用微信公众号"益心保甲"平台辅助学习在甲状腺外科学教学中的应用价值.方法:于2020年3月10日至25日,入选我校2017级临床医学五年制专业本科学生80人,麻醉学五年制专业学生10人,精神医学五年制专业学生10人,共计100人随机分为两组,实验组与对照组,每组各50人.实验组利用微信公众号平台预习及复习,对照组采用传统教材学习.利用"问卷星"平台发布考核测试,评价两种方法的辅助学习效果.结果:实验组学生授课前预习测试成绩与授课后复习测试成绩均优于对照组(P<0.05).结论:新冠肺炎疫情期间学生居家利用"益心保甲"公众号辅助学习甲状腺外科学的效果优于传统自学方法.

目的 系统评价微信平台在外科学教学中的辅助应用效果.方法 确定检索策略、原始文献的纳入与筛选原则,分别检索CNKI中国期刊全文数据库、中国生物医学文献数据库、万方数据库.检索文献主题为探讨微信辅助外科学教学对临床实习生及医学生的影响,实验组干预措施为利用微信平台辅助学习,对照组为传统教学方法.检索时间跨度为2014年4月1日-2020年4月30日.运用RevMan 5.3统计软件进行Meta分析.结果 纳入研究文献12篇,研究对象1354人,其中试验组685人、对照组669人.Meta分析显示实验组与对照组的理论知识考试成绩对比,加权平均差(WMD) =6.04,95％C/: 4.41～7.97,P＜0.00001,相较于对照组,实验组的理论考试成绩更高;2组技能测试考核成绩比较,WMD=5.63,95％C/:1.41～9.85,P＜0.01,实验组的技能考核成绩更高.结论 从外科学理论知识考试和技能考核的成绩看,利用微信辅助学习的实验组教学效果相比传统教学的对照组更好,因此可在新冠疫情教学改革中大力推行利用微信的多样性平台辅助医学生的外科学学习.

精准化对口医疗帮扶是我国进行可持续化医疗发展的要求,其医疗帮扶过程实质为组织教学的过程,因其教学对象的特殊性,常规教学模式收效欠佳.针对目前帮扶教学的现状,为提高教学成效,该院采用E-learning平台下翻转课堂的教学模式对被帮扶地区的医疗机构、相关医疗工作人员进行帮扶教学,从教学目标、教学设计、教学实施方案等多方面进行改进,并以血液科临床教学为示例,汇总并分享相应教学心得,对所存在的问题提出解决方案.虽然E-learning平台下翻转课堂的教学模式仍需进一步完善和验证,但仍可为帮扶教学的可持续发展提供相应教学经验参考.

BACKGROUND:Increasingly evidences suggest that long noncoding RNAs (lncRNAs) play important roles in various cancers. LncRNA PXN-AS1-L is recently revealed to act as on oncogene in liver cancer. However, the expression, functions, and mechanisms of action of PXN-AS-L in non-small cell lung cancer (NSCLC) remain unclear.METHODS:The expression of PXN-AS1-L in primary NSCLC tissues, NSCLC bone metastasis tissues, and cell lines was measured by quantitative real-time PCR. The correlations between PXN-AS1-L expression and clinicopathological characteristics of NSCLC patients were analyzed by Pearson Chi square test and log-rank test. The roles of PXN-AS1-L in cell viability, proliferation, apoptosis, and migration of NSCLC cells, and in vivo NSCLC tumor growth were investigated by a series of gain-of-function and loss-of-function assays. The regulatory roles of PXN-AS1-L on PXN were determined by quantitative real-time PCR and western blot.RESULTS:PXN-AS1-L was up-regulated in NSCLC tissues compared with noncancerous lung tissues, and PXN-AS1-L was further up-regulated in NSCLC bone metastasis tissues. Increased expression of PXN-AS1-L was positively associated with advanced TNM stages and poor prognosis. Gain-of-function and loss-of-function assays showed that PXN-AS1-L increased cell viability, promoted cell proliferation, inhibited cell apoptosis, and promoted cell migration of NSCLC cells. Xenograft assays showed that PXN-AS1-L also promoted NSCLC tumor growth in vivo. Mechanistically, we found that PXN-AS1-L, as an antisense transcript of PXN, up-regulated the expression of PXN. PXN was also up-regulated in NSCLC tissues. The expression of PXN and PXN-AS1-L was positively correlated in NSCLC tissues. Furthermore, PXN knockdown attenuated the roles of PXN-AS1-L in increasing cell viability, promoting cell proliferation, inhibiting cell apoptosis, and promoting cell migration of NSCLC cells.CONCLUSIONS:Our data revealed that PXN-AS1-L is up-regulated and acts as an oncogene in NSCLC via up-regulating PXN. Our data suggested that PXN-AS1-L might serve as a potential prognostic biomarker and therapeutic target for NSCLC.

Non-coding RNAs (ncRNAs) have been shown to regulate gene expression involved in tumor progression of multiple malignancies. Numerous studies have indicated that N-acetylglucosaminyltransferase V (MGAT5), is an important tumorigenesis and metastasis-associated enzyme in breast cancer (BC). But, the underlying molecular mechanisms by which ncRNAs modulate MGAT5 expression in BC remain undetermined. In this study, we demonstrated that miR-124 expression at a low level in BC tissue was associated with poor prognosis of BC patients. Meanwhile, miR-124 reduced BC cell proliferation and metastasis. MGAT5 was confirmed as a direct target of miR-124. MGAT5 restoration attenuated the inhibitory effects of miR-124 on BC proliferation and metastasis in vitro and vivo. Overall, we provide new insight into the mechanisms by which miR-124 inhibits BC progression, suggesting the potential of miR-124 and MGAT5 as biomarkers for early diagnosis of breast cancer to provide innovative ideas and methods for the diagnosis and treatment of BC.