Objective To know well the sanitary-epidemiological situations of the area of a training base .Methods The combination of field investigation with laboratory test was taken to investigate comprehensively the situations of geography and climate , water and environmental quality ,infectious disease and endemic disease ,medical insect and animal on the area of a training base .Re-sults Drinking water sources used all were in surface water ,and water quality was class Ⅲ.Indicators of oxygen consumption ,total bacteria count,total coliforms,thermotolerant coliforms,turbidity,aluminum,chloride in water exceed the standard.The sources of main foods were complex and there were many potential safety hazards .On epidemic situation,intestinal infectious diseases ,respiratory tract infectious diseases ,blood borne diseases ,sexually transmitted diseases accounted for the larger proportion of notifiable communicable diseases,and the proportions of vector borne diseases and natural focal diseases were less low .The main threat of epidemic was intesti-nal infectious diseases to training troops at this area ,secondly respiratory tract infectious diseases ,and the potential threat was epidemic hemorrhagic fever .The active period of main vectors was from May to November .Conclusion Troops training at this area should strengthen works of disinfection of drinking water and food safety ,and implement preventive measures of infectious diseases .

Objective:To explore the construction of digital hospital based on the technology of cloud computing.Methods: The construction of data center, deployment of desktop system, transformation of application model, management of information platform and training of staff under the condition of cloud computing will be discussed in this article as well.Results: Taking the technology of cloud computing as core, we built the foundation platform of cloud computing to explore the model of application and management as well as bring forth new ideas in services providing, which brings a competitive advantage for the development of hospital.Conclusion: The practice of construction based on the technology of cloud computing promotes the development for digital hospital, which improves the quality of information services.

The requirements of constructing military medical service information system were reported in the modern war .A conception of constructing Chinese military medical service information system for wartime was put forward , which included the overall design , construction tasks and guarantee measures .The detailed construc-tion scheme and measures from the basic network , software system, database , and medical equipment have been emphasized .

Objective: Through the implementation of Medical Cloud Project, solve resource utilization, daily operation and management, safety protection and other problems in information construction of military hospitals. Method: Cloud computing technology is used to realize virtualization of all kinds of resources on the digital hospital information basic platform, dynamically adjust and distribute each type of resource pool according to the needs, and establish a unified daily operation and safety protection management platform. Results: Through the application of cloud computing technology, the information infrastructure integration capabilities of hospitals are improved, and collaborative development of software, hardware and information security of hospital is realized. Conclusion: Supported by cloud computing technology, construction of Medical Cloud Project adheres to the top-level design and highlights technical innovation. It can effectively enhance the level of digital hospital construction of military, and improve and optimize the medical information security service abilities.

Objective To analyze the requirements on construction of the decision support system based on GIS for sanitary and anti-epidemic in military training bases. Methods A semi-structured interview method was taken to investigate the necessity,elements and development approach constructing the system. Results Our army does not have a decision support system specific for sanitary and anti-epidemic in military training bases. This system should be constructed because intensity of troops training relying on training bases was increasing currently in our army. The constructed system should be based on GIS to meet the needs of the troops,and was technically feasible. This system should include four parts as the geographic database of military medicine,the sanitary epidemiology database,the local health resources mobilized database and the expert knowledge database. Conclusion It is necessary and feasible for this system to be constructed. The main contents and features of the system should meet the needs of troops stationed in military training base. This constructed system should effectively enhance the ability of medical service for troops stationed.

本文介绍某军区充分发挥军队集中统管优势,建立完善军区医学期刊年会制的做法与体会以及成效。领导重视是办好年会的根本保证;行政机关主办是办好年会的关键因素;注重实效是办好年会的核心要求;以会代训是办好年会的重要方法。

The article introduced the experiences of monitoring network public sentiment in military prima-ry hospital , and discussed the monitoring and coping methods including developing a series of emergency preplans , optimizing the disposal process .The author also found some problems to solve , provide some references for monito-ring network public sentiment in military hospitals .

Objective:Through the analysis of the risk factors in overall development, data safety, medical behavior and daily operation of Medical Cloud Project in military hospital, the corresponding measures and solutions are proposed to reduce the safety risks in the project construction. Method: Combining the actual situation of information construction of the military hospital, the construction characteristics and implement method of Medical Cloud Project are fully analyzed. The possible impact of the application of cloud computing technology on information management, medical behavior and other aspects in military hospital is studied. Results:While cloud computing is used to improve the hospital' s ability of information construction, appropriate measures shall be taken to reduce the risks of the military hospital in overall development, data safety, the medical behavior and daily operation. Conclusion:By strengthening overall planning, highlighting data safety, establishing emergency mechanism, strengthening methods of technical guarantee and using other methods, risks can be reduced, and the military hospital' s medical information service guarantee ability based on cloud computing can be improved and optimized.

本文阐述了军队网络医疗建设的意义,分析了当前军队医院网络医疗建设的现状和存在的问题,并提出了对军队网络医疗建设的看法和建议。

部队卫生信息系统推广应用已10多年,为部队卫生工作规范化建设发挥了积极作用。本文通过调查统计,分析了军区部队卫生机构信息化建设现状,提出了具体对策。

The mobile of the chemical weapons abandoned by Japan is a high risk and high toxic,high-explosive work.By analyzing the Mobile destruction scene risk factors and medical support task,a medical support implementation plan was made,which maintained Mobile destruction personnel healthy,ensure the Mobile destruction mission accomplished.

OBJECTIVE:To investigate the effects of GnRH analogues GnRHa and GnRHant on the MAPK pathway in rat Leydig cells. METHODS:Rat Leydig cells were primarily cultured for 24 hours in vitro and serum-starved for 2 hours, followed by treatment with GnRHa (10(-7) mol/L) or GnRHant (10(-6) mol/L) for 0, 5, 15, 30, 60 and 90 minutes, with the 0 min group as the control. Then the protein levels of phosphorylated ERK (p-ERK) and phosphorylated p38 (p-p38) were detected by Western blot, and that of p-ERK determined by the same means after co-incubation of GnRHa or GnRHant with the PKC inhibitor GF109203X at 1, 5, 10 and 20 micromol/L. RESULTS:After stimulation of the Leydig cells with GnRHa or GnRHant for different times, the protein level of p-p38 showed no significant difference from that of the control group (P > 0.05). Then the Leydig cells were treated with GF109203X at different concentrations for 20 minutes and with addition of GnRHa for another 10 minutes. The level of p-ERK was significantly decreased (P < 0.05) by GF109203X at 10 and 20 micromol/L. Compared with the control, the p-ERK expression was increased by 65% at 15 minutes (P < 0.05) in the GnRHant stimulation group, by 81% (to the peak) at 30 minutes (P < 0.05), began to fall at 60 minutes, and returned to the base level at 90 minutes. The p-ERK level exhibited no significant difference from that of the control (P > 0.05) after treatment of the Leydig cells with different concentrations of GF109203X for 20 minutes and then with GnRHant for 30 minutes. CONCLUSION:The ERK MAPK activation induced by GnRHa depends on the PKC pathway, but not that induced by GnRHant. The p-38 MAPK pathway may not be involved in the effect of GnRH analogues on rat Leydig cells.

Objective To observe the expression of annexin 5 in testis of SD rats under constant light stress.MethodsSD rats were exposed to constant light to establish short(2d,3d,4d,7d)and long-term (14d,21d,28d) constant-light-stressed models.The testes were then obtained from the model rats and their corresponding controls respectively.Immunolocalization and relative expression of annexin 5 in rats' testes were analyzed by immunohistochemistry and Western blotting.ResultsWestern blotting showed that,compared with the controls,the expression of annexin 5 in the rats' testes of 14d and 21d group was upregulated significantly by about 34.5%±11% and 26%±16% (P<0.01,P<0.05),respectively,and no significant changes were observed in short (2d,3d,4d,7d) constant-light-stress groups and 28d group (by 18%±5%,P>0.05).Immunohistochemistry showed no changes in annexin 5 localization in rats' testes in stress groups compared with that in control groups.However,a stronger coloration of annexin 5 was observed by immunohistochemistry in 14d,21d and 28d constant-light-stress groups,implying a higher expression of annexin 5.ConclusionThe long-term (14d,21d) constant-light-stress may increase the expression of annexin 5 in rat's testis.Along with the prolongation of stress time (28d),with the development of compensatory mechanism,the expression of annexin 5 in rat's testis approaches the normal level,implying that annexin 5 might participate in the stress processes,as well as in the regulation of some important physiological functions.

Objective Previous research discovered that after stimulation of GnRH on Leydig cells, the expression of annexin 5 and the level of testosterone were increased, then we guess that there may be interaction between annexin 5 and testosterone secretion. 3β-HSD is a marker enzyme in Leydig cells.This paper is to study the effect of annexin 5 on testosterone secretion and 3β-HSD expression in cultured rat Leydig cells. Methods The primary rat Leydig cells were cultured in vitro and treated with different concentration of annexin 5 (0,10-10,10-9,10-8mol/L) and with 10-9mol/L concentration of annexin 5 at varied time courses (6, 12, 24,36 h).The culture media were collected for testosterone measurement by chemiluminescence assay. The expression of 3β-HSD protein were also analyzed by Western blot. Results The level of testosterone was significantly increased by 18%(14.38±0.30 vs 12.12±0.74)and 26%(15.25±0.47 vs 12.12±0.74)after treatment for 24 h with 10-10mol/L and 10-9mol/L concentration of annexin 5 (over control, P0.01) respectively. There is an increasing trend with treatment of annexin 5 (10-8mol/L)but no significant change(P0.05); Western blotting results showed the 3β-HSD expression was significant increased by 63%(2.19±1.00 vs 1.34±0.19)(P0.01) at 10-9mol/L concentration but no significant change at 10-10and 10-8mol/L concentration. There was a correlation between the level of testosterone and 3β-HSD expression(r=0.430). Compared with control group, after treatment with 10-9mol/L concentrations of annexin 5 for 24 h, the level of testosterone was significantly increased by 18%(12.59±0.41 vs 10.70±0.09)(P0.01), and decreased by 21%(14.44±1.65 vs 17.46±0.31)(P0.01)for 36 h after stimulation. But there were no significant changes for 6h and 12 h. Conclusion In rat Leydig cells, annexin 5 regulates testosterone secretion in a dose-and time-dependent manner. 3β-HSD is one of key enzymes in the process of estosterone secretion.

The development and wide application of assisted reproductive techniques(ART) help many couples suffered from infertility and/or sterility to get baby.Meanwhile,the usage of ART led to much ethic problem.We,hence,investigate the strategies to deal with the ethic problem in ART correctly.

Objective: To investigate the effects of the GnRH analogue on the expressions of annexin 5 and 3β-HSD mRNA in rat Leydig cells.Methods: Rat Leydig cells,after primarily cultured for 24 hours,were treated with different concentrations of GnRH agonist(10-5mol/L,10-6 mol/L,10-7 mol/L) and GnRH antagonist(10-6 mol/L,10-7 mol/L,10-8 mol/L).The expression levels of annexin 5 and 3β-HSD mRNA in the Leydig cells were measured by fluorescence quantitative RT-PCR.Results: Compared with the unexposed controls,the rats treated with GnRH agonist at the concentrations of 10-5mol/L and 10-6mol/L showed an increase in the expression of annexin 5 mRNA by 38.89%(P< 0.05) and 35.29%(P< 0.05),and those treated with GnRH agonist at 10-5mol/L had an increase of 46.43%(P< 0.01) in the expression of 3β-HSD mRNA.While GnRH antagonist significantly decreased the expression of annexin 5 mRNA by 45.45% at 10-6mol/L(P< 0.05),and reduced the expression of 3β-HSD mRNA by 63.64%(P<0.01) and 50%(P<0.05) at 10-7mol/L and 10-8mol/L.There were no statistically significant differences between the control and the other experimental groups.Conclusion: The GnRH analogue can regulate the expressions of annexin 5 and 3β-HSD in primarily cultured Leydig cells,probably by initiating a series of cell transductions after combined with the GnRH receptor.However,the underlying mechanism needs to be further studied.

如何认识现代国防卫生动员的要点与作用,需要搞清以下几个问题: 1 卫生动员的作用 卫生动员在战争中的作用是为了增进和维护军民的健康及战斗力[1].如何使战争中大量的伤病人员恢复健康,不仅仅涉及到战争的人力动员问题,而且直接影响到军民的情绪和斗志.

近年来,我们紧密联系部队计划生育工作的实际,着眼经常、突出重点、注重结合,以加强宣传教育,提供优质服务,改善生活条件,提高生活质量,建立文明幸福家庭为主线,广泛深入地开展了婚育新风进万家活动,部队官兵的新型婚育观念增强,自我生殖保健能力和家庭生活质量明显提高,促进了部队计划生育工作的进一步落实.