The fruit body of Lepista sordida cultivated with main substrate of tea tree sawdust was used as the experimental samples. The FAO/WHO pattern and amino acid ratios coefficient method were used to evaluate sample from the aspects of mineral and amino acid nutrition. The results showed that the sample is rich in Fe, Zn, Se and various amino acids, of which the Fe content was as high as 388 mg·kg-1. Except for As, which was slightly higher than the limit of As contaminants in edible fungi, the contents of Cd and Pb were all within the limits of standards. Umami amino acid＞sweet amino acid＞aromatic amino acid among the six flavor amino acid contents. Among them, the umami amino acid（Asp+Glu） accounts for 58.37%. By further analysis showed that the value of EAA/TAA was 0.31, and the EAA/NEAA was 0.46, which were all lower than the high-quality protein standard proposed by FAO/WHO. Thr and Lys were relative excess, Ile was the first limiting amino acid of thesample, the score of ratio coefficient of amino acid in the sample was 78. Although there was a gap between the sample and the model spectrum standard of the amino acids, they met the nutritional needs basically.

[Objective]The effects of different agents combinations of chlorphopham(CIPC),carvone and chlorine dioxide(ClO2)on the storage of sweet potato were explored to provide the scientific basis for its application in the production.[Method]Taking chlorine dioxide(ClO2)as the preservative,and carvone and CIPC as the bud inhibitor in the experiment,the effects of different combinations[non-preservative+non-bud inhibitor(A1B1),non-preservative+carvone(A1 B2),non-preservative+CIPC(A1B3),ClO2+non-bud inhibitor(A2B1),ClO2+carvone(A2B2)and ClO2+CIPC(A2B3)]on the storage of sweet potato were studied by two-factor experiment with randomized block design.[Result]With the prolonging of the storage,the germination rate of Ziyun red core sweet potato under six treatments showed an increasing trend.The germination rate of all treatments(except A2 B2)on 90 d was 1.23％-25.43％,among which A1B3 was the highest,A2B3was the lowest.The germination rate of all treatments on 105 d and 120 d was 2.97％-37.17％and 10.57％-62.03％,among which A1B3 was the highest,A2 B2 and A2B3were the lowest.The germination rate of all treatments on 135 d was 18.17％-100.00％,among which A1B1was the highest,A2 B2 was the lowest.Applying preservative could effectively inhibit weight loss rate and decay rate of sweet potato,while the effects of applying bud inhibitor were not obvious.When the Ziyun red core sweet potato were stored on 135 d,the average weight loss rate of different treatments was 27.72％-41.56％,following A1B1＞A1B3＞A1B2＞A2B2＞A2B1＞A2B3;The average decay rate was 11.00％-39.67％,following A1B1＞A1B2＞A1B3＞A2B1=A2B3＞A2B2.[Conclusion]During the storage period,the combined application of ClO2 and carvone could effectively inhibit the germination of tubers,reduce the weight loss rate of tubers and delay the deterioration of quality.ClO2 had good preservative effect and could be used in actual production.

目的:对比人工股骨头置换与股骨近端防旋髓内钉内固定在老年股骨转子间骨折中的应用效果及对髋关节功能的影响.方法:选取2016年1月至2021年2月本院收治的老年股骨转子间骨折患者98例,随机纳入对照组、观察组,各组49例.对照组接受股骨近端防旋髓内钉内固定治疗,观察组接受人工股骨头置换治疗,两组均随访12个月.比较两组手术相关指标、治疗1个月后疗效,术后不同时间点髋关节功能、血红蛋白丢失量、下肢相对长度、疼痛,术后随访期间并发症发生情况.结果:观察组完全负重时间、卧床天数、住院天数更短,术中出血量、住院费用相对更高,差异均有统计学意义(P<0.05).术后1周、1、3、6、12个月,观察组Harris功能评分较对照组更高(P<0.05).术后1 d对照组血红蛋白丢失量、视觉模拟评分(VAS)低于观察组(P<0.05);与术后1 d比较,术后7 d两组血红蛋白丢失量增加,但观察组在组间比较更低(P<0.05);两组VAS评分降低,且观察组在组间比较更低(P<0.05).观察组下肢相对长度在组间比较更低(P<0.05).术后随访期间,观察组术后并发症发生率为12.24％,与对照组的8.16％比较,差异无统计学意义(P>0.05).结论:相较股骨近端防旋髓内钉内固定,人工股骨头置换治疗老年股骨转子间骨折术中创伤大、费用更高,可缩短下肢相对长度,缓解术后疼痛,改善患者术后康复情况,促进关节功能的改善,且并未增加术后并发症的发生风险.

以采自贵州省安顺市镇宁自治县的野生翘鳞香菇(QL-1)为试材,采用子实体形态学和菌丝ITS序列分析法进行鉴定,研究了不同温度、pH、碳源、氮源、维生素、无机盐.和栽培基质对菌丝生长的影响,以期优化野生翘鳞香菇培养基和栽培基质,为进一步开发应用提供参考依据.结果表明:QL-1菌株为翘鳞香菇(Lentinus squrrosulus),培养的最佳温度为30℃,最适pH为5～6;最佳碳氮源分别为果糖和酵母提取物,菌丝生长速度最快,为1.07 cm·d-1和0.81 cm·d-1;添加麦芽糖和硝酸铵,菌丝的浓密度最佳;培养基中添加适量的维生素B1、维生素B6、硫酸锰和氯化钙能有效促进菌丝生长;在栽培基质中添加20％的玉米芯有助于菌丝体生长.

Objective To investigate the related factors affecting postoperative hidden hemorrhage in elderly patients with femoral intertrochanteric fracture（FIF） treated with proximal femoral nail antirotation（PFNA）.Methods A total of 120 elderly FIF patients who received PFNA treatment in the Department of Orthopedics of The First People’s Hospital of Xuzhou from May 2017 to May 2021 were selected as study subjects.Apparent,hidden,and total blood loss volumes after PFNA were calculated.The clinical data of all patients were statistically analyzed,and possible influencing factors were included.Univariate and multivariate analyses were used to find out the influencing factors of hidden hemorrhage after PFNA internal fixation in elderly FIF patients.Results The apparent,hidden,and total blood loss volumes after PFNA were（235.87±70.65） mL,（712.58±134.25） mL,and（947.77±208.91） mL,respectively.Univariate and multivariate logistic analysis results showed that age≥75 years old,unstable fractures,being complicated with hypertension or diabetes,operation time＞60 min,and preoperative anticoagulation therapy were all risk factors for the occurrence of hidden hemorrhage after PFNA internal fixation in elderly FIF patients（P＜0.05）.Conclusion Hidden hemorrhage is the main cause of perioperative blood loss in elderly FIF patients treated with PFNA internal fixation,and age≥75 years,unstable fractures,being complicated with hypertension or diabetes,operation time＞60 min,and preoperative anticoagulation therapy are important risk factors for hidden hemorrhage.Therefore,relevant clinical measures are suggested for elderly patients with FIF undergoing PFNA internal fixation to improve prognosis and reduce the occurrence of hidden hemorrhage.

为了探索榆黄菇菌丝生长的最佳条件,对榆黄菇菌丝生长的最适温度、pH值、培养基、碳源、氮源和光照条件进行了筛选.结果表明:榆黄菇菌丝生长的最适温度为25℃,最适pH值为6～7,最适培养基为PDA培养基,最适碳源为葡萄糖和蔗糖,最适氮源为硝酸钾,最适光照条件为黑暗.

对采自贵州省安顺市两株野生鸡腿菇(MT-1、MT-2)进行了形态及ITS序列分析鉴定,生物学特性初步研究和覆土栽培试验.结果 表明:MT-1、MT-2与鬼伞属的毛头鬼伞最大同源性高,为100％、99.38％;两菌株菌丝生长最适氮源、碳源分别为蛋白胨、甘露醇;MT-1最适pH为5～7,MT-2最适pH为7;两菌株菌丝最适生长温度均为25℃;光照对菌丝生长有一定的抑制作用,菌丝体在黑暗条件下长势最好;覆土栽培结果表明:MT-1、MT-2生物学效率分别为73.78％、54.54％.

金刺梨黑斑病主要于开花期为害幼嫩的果梗,果梗受害后产生黑色干腐病斑,导致整个花序从果梗处脱落.对黑斑病病原菌进行了分离和鉴定,通过形态学特性、致病性测定、rDNA-ITS序列分析,初步确定该病主要由拟茎点霉屑(Phomopsis Sacc.)的Phomopsisca psici真菌引起.

为了探索外施香菇菌发酵液对马铃薯种薯生长和品质的影响,本试验将培养7d的香菇菌发酵液(生物量达8.62mg/100mL)稀释100倍后,采用4种外施方法处理马铃薯种薯.结果表明,浸泡+撒施处理能明显促进马铃薯出苗与生长,提升品质;块茎干物质、淀粉、维生素C、还原糖及蛋白质含量分别为23.45g、17.68％、160.54mg/kg、0.84％和13.06％;植株退化率降幅为28.33％～43.33％;产量收益增加17.16％,该处理方式具有明显的生产利用价值.

目的:探讨下颌康复训练管理对下颌骨髁突骨折患者术后下颌康复的作用.方法:将42例患者按入院顺序分为对照组和干预组,对照组按照常规训练方法,干预组按照PDCA循环式训练管理方案,观察两组患者康复训练依从性,下颌骨功能恢复情况.结果:干预组下颌运动康复训练依从性高于对照组(<0.05);两组患者训练后1月、3月、6月主动张口度、前后活动度比较,差异有统计学意义(<0.05).结论:下颌运动康复训练管理有助于提高髁突骨折患者术后的依从性,促进下颌骨运动功能的恢复.

研究金刺梨黑斑病病原菌的生物学特性,并进行室内药剂毒力测定.结果表明,该菌最佳生长温度为28℃;最适pH值为4;供试碳源中果糖最适合菌丝生长;供试氮源中蛋白胨最适合菌丝生长;光照对菌丝生长有一定的影响,但连续光照能刺激菌丝生长;菌丝和孢子的致死温度分别为55、50℃.室内毒力测定结果表明,80％多菌灵可湿性粉剂对该菌菌丝的抑制作用最好,其EC50为0.56 mg/L.

近年来,随着农业产业结构调整力度的加大,安顺市香菇种植面积逐年增加,社会经济效益明显,但由于发展盲目性及粗放管理,导致香菇产量不高,产品质量良莠不齐.通过调查了解安顺市香菇产业发展现状,对香菇产业发展优势及制约因素进行分析,并提出发展建议,以期为安顺市香菇产业规范化、标准化生产提供一定的理论参考.

Residues of Letinous edodes were analyzed in the laboratory for their nutrient contents and applied to cultivate potato in the field to analyze their impact on production and quality of potatoes.The results showed that the residues of L.edodes were abundant in nutrients and contained high nutrients.Potato,when treated with residues of L.edodes at a rate of 1 752 kg/hm2,yielded 31.20 t/hm2,the highest,6.1％ higher than the control,and its plant height,stem diameter and leaf area were 72.4 cm,1.376 cm,7.06 cm2 and 31.20 t/hm2,respectively,obviously higher than those of the control.The quality of potato cultivated under the residues of L.edodes was also improved.

通过对安单系列玉米自交系进行不同施肥水平处理,测定农艺性状、生理指标及其产量,采用灰色关联分析方法对其进行评价,并筛选耐瘠鉴定的有效指标.结果表明:经灰色关联度分析,行粒数、茎粗、穗长、叶绿素、地上干物质重可以作为玉米自交系耐瘠性鉴定的有效指标;耐瘠性较强的自交系为K508、苏65、1441、引-10,较弱的自交系为异78、77、丹混、D902、78599.

In order to determine the best fertilization levels of tube seedlings of Radix Pseudostellariae, The experiment takes Radix Pseudostellariae plantlets for example, using L9 (34) orthogonal design for exploring the effect of different urea nitrogen concentration, calcium magnesium phosphate concentration and different sulfate potassium dosage on yield of plantlets of Radix Pseudostellariae at autumn. The results showed that when the urea amount to 450 kg/hm2, calcium magnesium phosphate application amount to 525 kg/hm2, sulfate potassium for 75 kg/hm2 (treatment 8), the yield was the highest, 2447.10 kg/hm2.

利用不同浓度的PEG-6000水溶液,在室内模拟玉米种子萌发及苗期水分胁迫程度,研究了安单3号、安单2号与安单4号3个品种种子萌发及苗期对水分胁迫的响应程度,比较了其抗旱性.结果表明,安单3号的抗旱性高于安单2号与安单4号,在土壤水分含量低的情况下,应选择种植安单3号,以保证玉米产量,减少经济损失.

[目的]筛选出拔节期和抽雄期影响玉米自交系产量的主要生理指标,为生理指标在玉米种质筛选或选育中的应用提供理论依据.[方法]分别于拔节期、抽雄期测定20个玉米自交系的6项主要生理指标,收获期测定产量及其相关性状,采用灰色关联法分析各生理指标与产量及其相关性状的关联度.[结果]在玉米拔节期和抽雄期,叶片相对水含量(RWC)、SOD活性与产量及其相关性状的关联度较高,拔节期两项指标与产量的关联度分别为0.9036和0.8489,抽雄期分别为0.7628和0.7223;拔节期的可溶性糖含量和抽雄期的叶绿素含量与产量的关系也较紧密,关联度分别达0.8311和0.6962.[结论]RWC、SOD活性、可溶性糖含量和叶绿素含量4项指标可作为玉米自交系筛选与种质资源创新中的参考指标.

Objective Explore the application effect of comminuted fracture of the jaw traction nail in the treatment of intermaxillaryand experience.methodsIn 2010 January to 2014 year in June in our hospital were randomly divided into comminuted mandibular fractures in 90 patients as control group and observation group, the control group after operation withintermaxillary traction nail treatment, observation group without the use of intermaxillary traction nail treatment. 3 to12 months after treatment, to evaluate the therapeutic effect of occlusion relationship, according to the opening degree of the jaw and X-ray.ResultsPatients in control group were treated with intermaxillary traction nail traction ifxation after treatment the total effective rate reaches as high as 93.3%, signiifcantly higher than those in the observation group was 77.8%, there was statistical significance of the difference between the two groups (P<0.05).ConclusionIntermaxillary traction nail in bone crushing an excellent performance in treatment of fracture, obviously helpto the recovery of the patients with occlusion, is widely used in clinic.

L 9 (34) orthogonaltestw asconducted com bined the factorsofsprouting reagent,soaking concentration and soaking tim e to study the besthandling m ethod forbreaking the restoffallbreeding potato seeds.The results show ed thatthe highestyield w as 724.93 kg/667m 2, w hich w assoaked in 5 m g/L ofG A 3 for10 m in ,increasing by 17.6% ~114.9% than thatofothers.These factors determ ining the yield can be queued asfollow s:sprouting reagent (A ) >soaking concentration (B ) >soaking tim e (C ) .

In order to determine the best nodes,sections and the density of cuttage of Ziyun virus-free sweet potato and provide a theoretical basis for its application,the effect of different nodes,sections and the density of cuttage on yield of Ziyun virus-free sweet potato were studied by the L9 (3 4 )orthogonal experiment.The results showed that the degree of various factors on the yield of decision was cutting nodes (A)> density of cuttage > sections (B),the highest production was 1900.79 kg/667m2 when the nodes was 12 bits,sections was 3,the density was 5417 / 667m2 ;the lowest production was 916.02 kg/667m2 when the nodes was 2 bits,sections was 4,the density was 3 334/ 667m2 .