目的：观察肾衰康口服液对阿霉素所致大鼠慢性肾衰竭模型的影响。方法通过单侧肾脏摘除合并两次注射阿霉素建立大鼠慢性肾衰竭模型，观察肾衰康口服液对大鼠体重、尿量、尿液中总蛋白（ TP）、内生肌酐清除率（ Ccr）、乳酸脱氢酶（ LDH）、碱性磷酸酶（ ALP），肾脏指数的影响。结果肾衰康口服液降低了慢性肾衰竭大鼠尿液中TP、LDH、肾脏指数；升高大鼠体重、尿量及Ccr。结论肾衰康口服液能有效改善慢性肾衰竭模型大鼠的肾脏衰竭状态。

Objective To study the influence of Shenshuaikang Oral Liquid on renal function and blood lipid metabolism in chronic renal insufficiency rats.Methods Chronic renal failure model in rats was established by removal of a kidney and injection of adriamycin.The mice were randomly divided into fiver groups,that is,normal control group,model group,Shenshuaikang Oral Liquid high,medium and low dose group and positive control group,n = 10.At the end of the experiment,we measured serum creatinine(Cr),blood urea nitrogen(BUN),total protein(TP),albumin(ALB),total cholesterol(TC),triglyceride(TG),kidney specimens were fixed with 10% neutral formalin,paraffin-embedded,sliced,HE staining and light microscopy.Results Compared with the model group,the values of Cr,BUN,TC,TG in each dose group decreased significantly,and those of TP,ALB elevated.The effects were dose-dependent and the role of high-dose group is best.Conclusions Shenshuaikang Oral Liquid can improve renal function,regulate blood lipid metabolism disorders and retard the progress of glomerular sclerosis;at last it can achieve the purpose of improving renal failure.

Objective To study the effects of shenshuaikang oral liquid on kidney-yang deficiency mice induced by hydrocortisone.Methods The kidney-yang deficiency model was induced by i.p.hydrocortisone in mice.Shenshuaikang oral liquid(30.0,15.0,7.5 g·kg-1)was orally administered once a day for 10 days.Effects of shenshuaikang oral liquid were detected on the body mass,body temperature,immune organs(thymus,spleen,adrenal gland),and genitals(foreskin gland,testis,prostate) in kidney-yang deficiency mice.Results The results showed that body weight,body temperature were improved significantly by shenshuaikang oral liquid at the dose of 30.0 g·kg-1 and the index of thymus,spleen,adrenal gland,prostate,testis and preputial gland were significantly increased.Conclusion Shenshuaikang oral liquid can significantly ameliorate kidney-yang deficiency induced by hydrocortisone in mice.

To determine the content of Astraloside in ShenShuaiKang Oral Liquor.Astraloside in ShenShuaiKang Oral Liquor was deter-minated by HPLC-ELSD,Use the Diamonsil-C18(4.6×250 mm,5μm) column,its mobile phase was acetonitrile-water(30:70).The flow rate was 1mL/min,drift tube temperature was 105℃a,nd gas flowrate was 2.5L/min.It was showed that the Astraloside had a good lineari-ty within the range of 0.4μg~4μg(r=0.999 5).The average recovery was 98.85 %,and RSD=1.65%(n=6).This method is simple and ac-curate for determination of Astraloside content in ShenShuaiKang Oral Liquor.

Objoctive To estabfish a quality standard of Shenshvaikang oral liquid.Methods Radix astragali were identified by the TLC,and Rhein in Shenshuaikang oral liquid was determined by HPLC.An ODS C_(18) column was used.The mobile phase was methanul-0.1%phosphoric acid (83:17).Detecting wavelength was 254nm.Results The TLC spots were developed fairly clear,and the blank test showed no interference;A good linearity was showed at the concentration range of 0.12～0.60μg of Rhein,the linear regression equation was y=2104 301.7x + 881.4,r=0.9998,and the average recovery rate was 98.98%,RSD was 1.26%,(n=5).Con- clusion This method is reliable,the results are stable with a good repeatability and can be used for quality controling standard of Shenshuaikang oral liquid.

目的研究煅牡蛎中钙离子在家兔体内的相对生物利用度。方法以葡萄糖酸钙溶液为标准参比物,测定给药后不同时间血清中钙离子浓度变化,经传统药动学方法计算药动学参数及相对生物利用度。结果钙离子在家兔体内代谢符合单室模型,Ka=0.6040,Ke=0.5227,Tmax=2.380h,Cmax=3.390μg·L-1,AUC=19.11μg·h·L-1。结论煅牡蛎中钙离子在家兔体内的相对生物利用度为142.5%。

Objective To study on the method of preparing bear gallbladder injection and to discuss its quality control.Methods The content of effective constitiuent(UDCA) in the bear gallbladder injection was tested by using the HPLC(the buffer solution was 0.3mol/ LNaH2PO4;mobile phase was(15:60:25);detecting wavelength was 205nm.Result The peak area and its content of UDCA presented a good linear relation between 19.24~96.20ug and the average recovery rate was 99.35%,RSD was 0.31%.Conclusion The bear gallbladder injection can be used in clinic since it meets the pharmaceutical dosage needs in hospital and the quality control method is simple,quick,accurate and reliable.

目的研究中药复方制剂利脑宁的镇痛作用,为该药的临床应用提供理论依据。方法通过小鼠扭体法、小鼠热板法、大鼠电刺激法进行镇痛实验。结果利脑宁可显著抑制醋酸所致小鼠扭体反应,可提高热板法所致小鼠的痛阈及大鼠电刺激法所致的痛阈,其作用强度呈剂量依赖性。结论利脑宁具有镇痛作用。

AObjective To observe the effect of the compound jujube oral liquid on immune function of rats .Methods Rats were divided into two groups: control group and experimental group. The rats' thymus index, production of their serum agglutinin and macrophoge's phagocytic function were tested. Results The compund oral Liquid has a tendency to make thymus index go up, increase the content of serum agglutinin (P0.01) and develop the phagocytic function of macrophage in a rat's cavity (P0.01). Conclusion The compound oral liquid can obviously develop immune function of organism.

目的:研究复方左氧氟沙星滴耳剂质量控制、临床应用.方法:以左氧氟沙星为主药,辅以醋酸地塞米松,使用紫外分光光度法测其含量.结果:该制备工艺可行,无刺激性,平均回收率100.07%,RSD 0.37%(n＝5).结论:该制剂临床疗效显著.

目的:建立曲克芦丁注射液的细菌内毒素检查法.方法:按<中华人民共和国药典>2000年版二部收载的细菌内毒素检查法进行试验. 结果: 干扰试验表明,曲克芦丁注射液对鲎试验有干扰,但经12倍稀释后可排除干扰,用灵敏度为0.25 EU·mL-1的鲎试剂检查细菌内毒素是有效的.结论:细菌内毒素检查法可以替代家兔法来控制曲克芦丁注射液的质量.

目的研制复方左氧氟沙星凝胶剂,制定质量控制标准.方法以卡波姆-940为主要凝胶基质,左氧氟沙星、替硝唑为主药制备凝胶剂.采用紫外分光光度法同时测定左氧氟沙星和替硝唑的含量.结果制备的凝胶剂均匀细腻,粘附性好,质量稳定,左氧氟沙星在3.0～8.0μg·ml-1范围内线性关系良好,替硝唑在12.0～32.0μg·ml-1范围内线性关系良好,左氧氟沙星和替硝唑平均回收率分别为100.15%、100.14%,RSD分别为0.84%、1.31%.结论制剂制备工艺简单,含量测定操作简便,结果准确.

慢性肾功能衰竭(CRF)的有效治疗方法,在于延缓慢性肾功能损害的进程.我院对 433 例非透析治疗 CRF 患者采用自拟方尿毒康煎剂进行治疗,同时设肾安注射组为对照进行观察,结果表明尿毒康煎剂能有效地延缓 CRF 的病情发展,疗效显著.

目的:制备复方氟罗沙星滴鼻剂.方法:以氟罗沙星配伍盐酸麻黄碱,用羧甲基纤维素钠和吐温-80作助悬剂.采用紫外分光光度法测定氟罗沙星的含量,旋光法测定盐酸麻黄碱的含量.结果:氟罗沙星测定的标准曲线为C＝9.679A+0.099,r＝0.999 8,平均回收率和RSD分别为100.35%和0.37%;盐酸麻黄碱测定的标准曲线为α＝0.730 0 C-0.018 0,r＝0.999 4,平均回收率和RSD分别为100.57%和0.61%.结论:制备的滴鼻剂符合医院制剂规范,质量控制方法可靠,可供临床使用.

目的: 应用尿毒康治疗慢性肾衰(CRF)并以肾安作对照观察临床疗效. 方法: 确定有慢性肾衰的患者,随机分为治疗组和对照组. 治疗组口服尿毒康 20 mL,3次*d-1;对照组肾安注射液250 mL,1次*d-1. 所有病例均常规服用潘生丁、碳酸氢钠、碳酸钙. 结果: 尿毒康有改善临床症状,使血肌酐(Scr)、尿素(Urea)降低,血红蛋白(Hb)和二氧化碳结合力(CO2CP)升高的作用,且明显优于肾安组. 结论: 尿毒康可增强患者免疫功能,抑制机体代谢,从而保护残余肾单位,有效延缓CRF 病情的进展.

1 材料与方法①实验动物及细胞株:G57小鼠2只,雌雄不限,体重18 g,由中国医学科学院实验动物研究中心提供;小公牛主动脉内皮细胞、白血病HL-60细胞、食道癌细胞、人结肠癌HCT-8 细胞均由中国医学科学院药物研究所提供.

目的:探讨国产氨苄西林所致药疹的临床类型及影响因素.方法: 收集氨苄西林所致的128例药疹患者的临床资料,按有关文献分类,并分析产生药疹的因素. 结果: 128例患者中麻疹样皮疹46.1%,猩红热样皮疹19.5%,多型红斑型14.8%,荨麻疹10.9%,红皮病型3.9%,紫癜型3.1%,大疱性表皮样松解萎缩坏死型1.6%.与文献报道的分类和所占比例基本一致.结论:氨苄西林所致药疹在临床上绝大多数为迟发性过敏反应,除个体因素外,均与药品的纯度、溶媒的pH值、药品的用量、药液的存放时间有关.

目的:提高左氧氟沙星生物利用度.方法:以甘油明胶为基质制备左氧氟沙星泡腾栓,建立质量控制并对其进行临床疗效观察.结果:该剂型制备工艺可行,无刺激性,质量控制方法可靠,平均回收率100.02%,RSD为0.32%;对各种感染性阴道炎有效率达100.0%.结论:该制剂可用于临床.

目的观察自拟方复方大黄糊敷脐对老年便秘患者首次排便时间的影响.方法按现代制药工艺将大黄、红花等中药制成糊剂;将复方大黄糊贴敷脐部治疗老年习惯性便秘、脑卒中便秘,并与单味大黄糊敷脐组进行对比.结果复方大黄糊各组首次排便时间显著优于单味大黄糊各组的通便效果(P＜0.01).结论复方大黄糊敷脐对各种便秘患者均具有显著的缓泄通便作用.