A portable dual-mode PDMS-based microfluidic chip aptasensor was developed to detect bovine pregnancy-associated glycoproteins (bPAG) in bovine milk. Reagents within the chip chambers underwent reactions driven by gravity, where pre-encoded rich C sequences on the complementary strand of the aptamer facilitated the generation of abundant G-quadruplexes via subsequent RPA reaction, which activated the chromogenic substrates and fluorogenic precursors in the chip, producing distinct colorimetric and fluorescent signals. These signals were captured by our developed smartphone application and converted into RGB values, further enabling the quantification of bPAG with detection limits of 0.079 ng/mL and 0.024 ng/mL for colorimetric and fluorescent modes, respectively, over a linear range of 0.1-100 ng/mL. Bovine milk and other animal source milk were evaluated in the proposed assay, accurate identification results were obtained, indicating significant potential in bovine milk monitoring. The work further provided a valuable reference for point-of-care testing of non-nucleic acid targets in food samples.

Exosome microRNAs are vital for gene transcription and expression, influencing target genes. They may play direct and indirect roles in pregnancy across animal models and humans. The objective of this research was to comprehensively explore the expression profiles of plasma exosomal miRNAs during the early pregnancy stage in sheep. This study specifically delved into the differences in the expression of plasma exosomal miRNAs between Chinese Merino and Suffolk sheep. The primary focus was on clearly elucidating the changes in plasma exosomal miRNAs, pinpointing potential biomarkers that might exert an impact on early pregnancy, and thoroughly exploring their biological functions as well as the possible underlying molecular mechanisms. Chinese Merino and Suffolk sheep were divided into two groups of 20 for artificial insemination. Blood samples were collected at various pregnancy stages (day 0, day 14, and day 30), Ultrasound diagnosis was carried out five weeks following artificial insemination. Three pregnant sheep from each group were selected for isolating extracellular vesicle-derived miRNAs from plasma. High-throughput sequencing identified differentially expressed miRNAs, and their target genes were analyzed using bioinformatics methods. In plasma exosomal samples derived from all sheep of both breeds at various time points during the early pregnancy stage, a cumulative total of 80 known miRNAs and 182 novel miRNAs were successfully identified. Compared to day 0 gestation controls, both sheep breeds showed differential expression of six known miRNAs: four were up- regulated and two down- regulated. Pathway analysis linked these miRNAs to biological processes such as ECM receptor interactions, growth hormone synthesis, carbohydrate digestion, adrenergic signaling in cardiomyocytes, and MAPK signaling regulation. Notably, Oar-mir-27a differed between the two groups at various time points. In summary, this research meticulously carried out a profiling analysis of the expression of plasma exosomal miRNAs in Chinese Merino and Suffolk sheep. Through this analysis, several exosomal miRNAs were identified, which display distinct abundance levels during the early pregnancy period. These findings strongly imply that these miRNAs have the potential to serve as biomarkers for precisely assessing the reproductive status of sheep.

Selenium (Se) is a beneficial element for plant growth and development. In this study, three-year-old potted 'Qingcui' plums were treated with Na2SeO4 (Se6+) or selenomethionine (SeMet Se2-) to explore the effect of Se on the plant growth, fruit quality, and 5-hydroxytryptophan (5-HTP) metabolism. Flower and fruit numbers, fruit quality and yield, Se content, and 5-HTP metabolites and enzymes were detected. The results showed that the flower and fruit numbers, and yield were significantly increased by the Se application. There were no significant differences in the fruit diameters, fruit mass, edible part ratio, titratable acids, water content, and solid acid ratio among the treatments. The total soluble solids, soluble protein, and malondialdehyde contents under the Se6+ treatment showed no significant difference compared to the Se2- treatment, but they were significantly higher than these under control by 16.71%, 39.13%, and 36.27%, respectively. The Se application markedly increased plant the Se content, and Se contents in the roots and leaves, or the fruits were significantly larger by the Se6+ treatments than the Se2- treatment. The leaves' pigment contents under the Se2- treatments were significantly larger than those under the control or Se6+ treatment. Tryptophan was not significantly influenced, the 5-HTP and 5-methoxytryptophan contents were reduced in the roots, and increased in the leaves, and the serotonin content was only significantly increased in the roots by the Se treatments. The tryptophan hydroxylase and hydroxyindole-O-methyltransferase levels were slightly influenced, and the tryptophan decarboxylase level in the roots or fruits was significantly increased by the Se treatments. The Se application had beneficial effects on the plant growth, fruit quality, and Se content, especially in the Se6+ treatment, and influenced the 5-HTP metabolism.

Background: Maillard reaction involves the polymerization, condensation, and other reactions between compounds containing free amino groups and reducing sugars or carbonyl compounds during heat processing. This process endows unique flavors and colors to food, while it can also produce numerous hazards. Acrylamide (AAm) is one of Maillard's hazards with neurotoxicity and carcinogenicity, these effects can trigger mutations and alternations in gene expression in human cells and accelerate organ aging. An accurate and reliable acrylamide detection method with high sensitivity and specificity for future regulatory activities is urgently needed. Results: Herein, we constructed a colorimetric aptasensor with the hybridization of MIL-glucose oxidase (MGzyme)-cDNA and magnetic nanoparticle-aptamer (MNP-Apt) to specifically detect AAm. The incorporation of MB-Apt and AAm released MGzyme-cDNA in the supernatant, took the supernatant out, with the addition of glucose and TMB, MGzyme would oxidize glucose, the resulting center dot OH facilitated the oxidation of colorless TMB to blue ox-TMB. The absorbance value at 652 nm, which indicates the characteristic absorption peak of ox-TMB, exhibited a proportion to the concentration of AAm. MGzyme avoided the addition of harmful intermediate H2O2 and created an acid microenvironment for the catalytic reaction. MNP-Apt possessed the advantages of high specificity and simplified separation. Under optimal conditions, this method displayed a linear range of 0.01-100 mu M with the limit of detection of 1.53 nM. With the spiked analysis data cross-verified by ELISA kit, this aptasensor was proven to specifically detect AAm at low concentrations.

A truncated aptamer (designated A24-3) was identified that specifically binds to bovine pregnancy-associated glycoproteins (bPAG9) with a low dissociation constant (2.04 nM) through two truncation approaches. Circular dichroism spectroscopy indicated that A24-3 formed parallel G-quadruplexes, which was subsequently confirmed using nuclear magnetic resonance (NMR) spectroscopy. Furthermore, a molecular dynamics simulation was employed to investigate the recognition mechanism of A24-3 and bPAG9. Interaction analysis showed that A24-3 folded into a parallel G-quadruplex structure with three G-tetrads, primarily through numerous hydrogen bonds and hydrophobic and π-π interactions. Finally, a novel colorimetric aptasensor was developed for detecting bPAG9 using A24-3 and an Fe-based metal–organic framework as target recognition elements and enzyme mimics, respectively. The method demonstrated a broad detection range from 0.5 to 50 ng/mL, with a low detection limit of 0.03 ng mL−1, and exhibited a good recovery (91.0–102

A Label-free colorimetric assay for the determination of tetracyclines detection based on aptamer and gold nanoparticles. The developed assay was applied to detection tetracyclines in milk samples, and was validated using GB standard method（GB 31658.6-2021）. The results demonstrated that, the samples were extracted with methanol+acetronitrile（6:4, V/V） and determined by the proposed colorimetric assay.Under the optimal conditions: the concentration of aptamer at 150 nmol/L, the concentration of NaCl at 40 mmol/L, the reaction time was 5 min,tetracyclines were measured with the linear concentration range of 0.25 to 5.0 ng/m L（R＞0.981）. The limit of visual detection was 0.5～1.0 ng/m L by naked-eye observation. The detection time was 5 min. The proposed colorimetric assay has high specificity for tetracycline,oxytetracycline, doxycycline, chlortetracycline, and no cross-reactivity towards chloramphenicol and gentamicin. Furthermore, the colorimetric assay was employed to determine tetracyclines in milk samples and recoveries ranged from 63.52% to 102.48%, with relative standard deviations of 2.49%～6.81%. The proposed methods possess the merits of simplicity, rapidity, low cost and high sensitivity, which could be a potential screening tool for the detection of tetracyclines in food samples.

创新决胜未来,青年大学生是推动大众创业、万众创新的生力军,而高校是培养适应时代需求的创新人才的重要载体.面对经济结构调整、产业升级、生产服务一线应用型、复合型、创新型人才紧缺等问题,近年来,国务院办公厅印发的《关于深化高等学校创新创业教育改革的实施意见》和教育部印发的《国家级大学生创新创业训练计划管理办法》等有关文件均明确提出,要转变教育思想观念,加强创新创业教育改革,完善大学生创新创业教育体系,提高人才培养质量.因此,大学生创新创业能力培养已经成为我国高等教育改革的大势所趋.

Herein, a SiO2@Ag NPs core/shell nanoparticles were synthesized to fabricate a surface-enhanced Raman spectroscopy (SERS) sensor for the simultaneous determination of histamine (HIS) and tyramine (TYR) based on specific aptamer recognition and ratiometric strategy. SiO2@Ag NPs with 4-thiosaminophenol (4-ATP) and Nile blue A (NBA) molecules were used as an internal standard (IS) and labeled with aptamers corresponding to HIS and TYR, respectively. Raman reporter molecules ROX and Cy5 labeled complementary DNA (cDNA) were then hybridized with aptamers to form rigid double-stranded DNA. After the HIS and TYR were captured by their aptamers, resulting in the dissociation of cDNA and separated from the SERS substrate. Therefore, the SERS signal intensity at 1503 cm-1 of ROX and 1358 cm-1 of Cy5 tagged on the terminal of cDNA decreased with the concentration of HIS and TYR increasing, while the SERS signal intensity at 1079 cm-1 of 4-APT and 592 cm-1 of NBA on the substrate remain stable. Thus, the concentrations of HIS and TYR can be determined by the I1503/I1079 and I1358/I592 values, respectively. This sensing strategy achieves a lower detection limit of 0.2 ng/mL for HIS and 0.05 ng/mL for TYR, respectively, demonstrating promising applications in sensitive detection of BAs in animal-derived foodstuff.

本研究以苏丹红Ⅲ适配体为识别元件，以未修饰的纳米金传感信号，以聚二烯丙基二甲基氯化铵（PDDA）作为纳米金聚集的诱导剂，构建了一种简单、经济、快速的苏丹红比色检测方法。在优化条件下评估本方法的检测灵敏度、准确性和特异性，最后应用于食品中苏丹红快速检测，并将检测结果与国标法（GB/T19681-2005）对比验证。结果显示，在PDDA浓度20 nmol/L、适配体浓度5 nmol/L、反应时间4 min等优化条件下，纳米金吸光度比值(A 620nm /A 530nm )与苏丹红III浓度呈良好线性关系（R=0.986），线性检测范围为3.13～50 ng/mL，可视化检测限为3.13 ng/mL，检测时间约为5 min。特异性分析显示，本方法对苏丹红I、II、III和IV有高的特异性，与柠檬黄、日落黄、分散橙11等无交叉反应。将本方法应用于食品中苏丹红检测，加标回收率为85.4%～102.5%，相对标准偏差为3.37%～6.75%。本方法具有操作简便、快速、结果易读等优点，适用于批量样品中苏丹红的现场快速检测。

旨在异源表达羊妊娠相关糖蛋白5(ovPAG5),并对其蛋白结构及功能进行预测分析,为ovPAG5抗体制备和羊早孕诊断技术的研发提供技术支撑.利用生物信息学方法对ovPAG5基因的编码序列进行密码子偏好性分析,根据宿主细胞HEK293的密码子偏好性对ovPAG5基因进行优化,全基因合成后构建重组表达载体pcDNA3.1(-)-ovPAG5,转染至HEK293细胞中诱导表达重组蛋白并纯化,进一步利用生物信息学软件对ovPAG5蛋白的结构及功能进行预测.结果显示,ovPAG5基因有效密码子数(NEC)和密码子适应指数(CAI)分别为51.91和0.78,表明该基因密码子选择偏性较弱.优化后ovPAG5基因密码子适用指数(CAI)由0.78提高到0.96,GC总含量由48％提高到57％,并成功合成优化的ovPAG5基因,大小与预期结果相符.重组质粒测序结果显示,其核苷酸序列与优化后基因完全一致,氨基酸未发生突变.表达的重组蛋白ovPAG5经SDS-PAGE和Western Blot鉴定分析,其相对分子质量约52 kDa.生物信息学预测分析结果显示,ovPAG5蛋白具有2个糖基化修饰位点、12个B细胞抗原表位以及31个氨基酸残基组成的信号肽.其二级结构由无规则卷曲(40.81％)、延伸链(31.74％)、α-螺旋(22.17％)和β转角(5.29％)组成.本研究为今后.vPAG5抗体的制备和羊早孕检测技术的研发奠定了基础.

In this study, a simple, economical and rapid colorimetric assay was developed for the detection of Sudan, in which Sudan-binding aptamer was used as recognition element, unmodified gold nanoparticles (AuNPs) as sensing signal, and poly(diallyldimethylammonium chloride) (PDDA) as inducer for gold nanoparticle aggregation. The sensitivity, accuracy and specificity of developed method were evaluated under optimized condition. Finally, the colorimetric sensor was applied to detection Sudan in food samples, and the results were compared with GB standard method (GB/T 19681-2005). The best procedure for Sudan analysis in our system was: The concentration of aptamer at 5 nmol/L, the concentration of PDDA at 20 nmol/L, and the reaction time was 4 min. The correlation between concentration of Sudan III and absorbance ratio of gold nanoparticles (A650nm/A530nm) was observed to be linear within the range of 3.13 to 50 ng/mL. The limit of visual detection was 3.13 ng/mL by naked-eye observation. The detection time was 5 min. The colorimetric sensor had high specificity for Sudan I, II, III and IV, and no cross-reactivity towards sunset yellow, tartrazine, and 1-amino-2-methylanthraquinone. Further, the colorimetric sensor was applied to measure Sudan Ⅲ in spiked real samples, and the recoveries were in the range of 85.4%~102.5%, with relative standard deviations of 3.37%~6.75%. Our study provides a simple, fast, and easy to read method for Sudan analysis, which can be applied in future on-site detection in food samples.

以苏丹红Ⅲ核酸适配体为识别元件,以无标记的纳米金为颜色指示剂,NaCl溶液作为纳米金聚集诱导剂,构建了一种简单、快速、灵敏的苏丹红可视化比色检测方法.对适配体浓度、NaCl浓度、反应时间等条件进行优化,并对该方法的灵敏度、准确性、特异性进行评估,将其应用于食品中苏丹红Ⅲ的快速检测,并与国标法对比验证.结果显示,在适配体浓度100nmol/L、NaCl浓度40 mmol/L、反应时间10min等最优条件下,纳米金吸光度比值(A620/A520)与苏丹红浓度在1.8～57.6ng/mL范围内呈良好线性关系(R2=0.981),方法检出限范围0.76～1.12ng/mL,裸眼可视化检出限为7.2ng/mL,检测时间为12min.特异性分析显示,本方法对苏丹红Ⅰ,Ⅱ和Ⅳ有交叉反应,表明可以用于检测苏丹红Ⅰ～Ⅳ.食品中苏丹红Ⅲ加标回收率为82.1％～94.7％,相对标准偏差3.9％～7.7％,与国标方法相比无显著差异(P＞0.05).该方法适用于批量样品中苏丹红的快速检测.

以苏丹红III核酸适配体为识别元件,以氯化血红素/G-四链体DNA酶为信号探针,结合杂交链反应信号放大策略,构建一种简单、新颖、高灵敏的生物传感器,用于苏丹红比色检测.在优化条件下,对该方法的灵敏度、准确性、特异性进行评估,最后将其应用食品中苏丹红IH快速检测,并与GB/T 19681-2005法对比验证.结果显示,在优化条件下,苏丹红HI质量浓度在0.5～250 ng/mL范围与450 nm波长处的吸光度呈良好线性关系(相关系数为0.995),方法检测限为0.09 ng/mL.特异性分析显示,本方法可用于苏丹红Ⅰ～Ⅳ的检测.实际样品分析中表明,食品中苏丹红III加标回收率为84.3％～101.6％,相对标准偏差为4.13％～8.36％,本方法的加标回收率与GB 19681-2005法相比差异不显著(P＞0.05).该方法操作简便、准确可靠、灵敏度高,适用于批量食品中苏丹红的快速检测.

为建立一种简便、经济、无创的应用于绵羊早期妊娠诊断的快速检测技术,以绵羊妊娠相关糖蛋白7(O-vine pregnancy-associated glycoproteins-7,ovPAG7)核酸适配体为分子识别探针,建立一种间接竞争酶联适配体(Indi-rect competitive enzyme-linked aptamer assay,ic-ELAA)检测ovPAG7的新方法,并对ovPAG7包被质量浓度、包被缓冲液、封闭条件、适配体浓度、链霉亲和素-辣根过氧化物酶(SA-HRP)稀释比例等检测条件进行优化,将建立的方法应用于人工授精后28 d的中国美利奴羊血清检测,检测结果与妊娠相关糖蛋白-酶联免疫吸附测定(PAG-ELISA)商业化试剂盒和B超检测法进行比较.结果显示,在ovPAG7包被质量浓度为2.0μg/ml、包被缓冲液为50 mmol/L碳酸盐缓冲液(pH 9.6)、1.0％牛血清蛋白质封闭2 h、适配体浓度为40 nmol/L、SA-HRP稀释比例为1:40000(体积比)、室温下竞争反应时间为40 min等优化条件下,ovPAG7在缓冲液体系和血清体系中质量浓度的线性范围均为0.1～50.0 ng/ml(R2>0.994),半数抑制质量浓度(IC50)分别为1.40 ng/ml和2.11 ng/ml,检测限分别为0.11 ng/ml和0.19 ng/ml.ovPAG7在空白样品中加标回收率为96.4％～106.8％,相对标准偏差小于6.0％.妊娠诊断结果显示,ic-ELAA法的诊断敏感性、特异性和准确率分别为96.0％、83.3％和91.2％,与B超检测结果一致性较高(Kappa值=0.810).

The pregnancy-associated glycoproteins (PAGs) have been widely used as biomarkers for the early diagnosis of pregnancy in cattle and sheep. This study aimed to obtain the single-stranded DNA aptamers that specifically bound to ovine pregnancy-associated glycoprotein 7 (ovPAG7) with high affinity (Kd = 9.8-32.4 nM) using real serum sample-assisted FluMag-systematic evolution of ligands by exponential enrichment (SELEX). Subsequently, the selected aptamers were applied to fabricate an ultrasensitive colorimetric aptasensor for ovPAG7 detection based on functionalized magnetic microparticles and hybridization chain reaction. Under the optimized conditions, the colorimetric aptasensor exhibited a broad linear range (0.2 -500 ng mL-1), low detection limit (0.081 ng mL-1), good recovery rate (94.5-109.1%), and high repeatability (relative standard deviation of 4.02-8.16%) in ovPAG7-spiked serum. Furthermore, this aptasensor was applied to measure the ovPAG7 in serum samples of ewes for pregnancy diagnosis. Blood samples were collected from Chinese Merino ewes on days 22, 28 after artificial insemination (AI) for ovPAG7 detection, respectively. Transrectal ultrasonography diagnosis of pregnancy 45 days after AI was the reference (gold) standard for all PAG tests. Diagnostic sensitivity, specificity, and accuracy of the proposed aptasensor were 95.8, 87.5, and 92.5% at day 22 and 95.8, 90.6, and 93.7% at day 28, respectively. The degree of agreement (Kappa) between developed aptasensor and ultrasonography diagnosis 22 and 28 day after AI were higher than 0.8. These results illustrated that the aptasensor was proved to be a sensitive, reliable and cost-effective way of measuring PAG and might be a useful means of pregnancy detection in ewes. (c) 2021 Published by Elsevier B.V.

该研究以金黄色葡萄球菌核酸适配体为识别分子,结合杂交链式反应和酶催化双重信号放大策略,建立了一种金黄色葡萄球菌高灵敏可视化检测方法.结果显示,在适配体包被浓度60 nmol/L、检测探针浓度80 nmol/L、发夹DNA浓度80 nmol/L、10 g/L牛血清白蛋白封闭4 h、链霉亲和素-辣根过氧化物酶稀释体积比1:40000、靶标与适配体反应时间30 min、发夹DNA杂交时间60 min等优化条件下,金黄色葡萄球菌在10～105 CFU/mL呈良好线性(R=0.992),可视化检测限为10 CFU/mL,与其余食源性致病菌无交叉反应.将建立的方法应用于食品中金黄色葡萄球菌检测,并与国标法对比验证,两种方法的检测结果无明显差异(P>0.05),加标回收率为91.8％ ～98.2％,相对标准偏差<6％.该研究所建方法前处理简单、灵敏度高、检测成本低、准确性好,适用于批量样品中金黄色葡萄球菌的快速筛查.

本研究以金黄色葡萄球菌为检测靶标,以核酸适配体为识别分子,基于双适配体夹心和侧流层析原理,构建了定性检测金黄色葡萄球菌的适配体试纸条,并对NaCl浓度、适配体浓度、纳米金-适配体包被量及捕获探针包被量等实验条件进行优化,获得适配体试纸条最佳制备条件.在优化条件下对试纸条的灵敏度、特异性进行分析测试,最后将试纸条对116份食品进行金黄色葡萄球菌检测,并与国标法(GB 4789.10-2016)对比验证.结果显示,适配体试纸条最佳制备条件为NaCl浓度为80 mmol/L、适配体偶联浓度为1μmol/L、结合垫上纳米金-适配体的稀释体积比为1:2、捕获探针浓度为25μmol/L.在最佳条件下,适配体试纸条对金黄色葡萄球菌的可视化检测限为2×103 CFU/mL,检测时间为5 min,且与其他食源性致病菌如鼠伤寒沙门氏菌、单增李斯特菌、大肠杆菌、阪崎肠杆菌、痢疾志贺氏菌等无交叉反应,具有较高的特异性.将本方法应用于食品中金黄色葡萄球菌的定性检测,检测结果与国标法完全一致.该方法简便快速、准确可靠、成本低,适用于食品样品中金黄色葡萄球菌的定性检测.

[目的]对bPAG16基因进行优化和合成,构建proEM-bPAG16重组表达载体,将重组质粒转染至HEK293细胞中,获得bPAG16重组蛋白,为家畜早期妊娠诊断技术的研发提供技术支撑.[方法]通过生物学技术对bPAG16基因密码子进行优化和人工合成,经T4?DNA连接酶将bPAG16基因插入到proEM载体中,构建重组载体proEM-bPAG16,将其转染至HEK293细胞中进行瞬时表达,重组蛋白经Ni-IDA亲和柱纯化后,采用SDS-PAGE、Western?Blot检测其表达效果.[结果]优化后的bPAG16基因密码子适用指数(CAI)由原来的0.77提高到0.96,GC含量由48％提高到58％.重组载体proEM-bPAG16双酶切后分别获得1179?bp和4369?bp的2条片段,与预期值一致;重组质粒测序结果显示,其核苷酸序列与优化后基因完全一致,氨基酸未发生突变;SDS-PAGE和Western?Blot鉴定结果显示,获得重组蛋白bPAG16(rbPAG16)相对分子质量约48?kDa,经Ni-IDA亲和层析纯化后,其纯度达到90％以上.[结论]通过密码子优化策略及重组蛋白技术高效表达了rbPAG16,为奶牛早孕快速检测技术的研发奠定了基础.

免疫分析测定妊娠相关糖蛋白(pregnancy-associated glycoproteins,PAGs)是目前常用的家畜早 期妊娠检测方法,但是抗体作为识别分子存在制备周期长、成本高等缺点.为丰富奶牛(Bostaurus)早期妊娠诊断技术,降低检测成本,本研究以牛(bovine)妊娠相关糖蛋白(bPAG)的核酸适配体为识别分子,基于双适配体夹心原理,建立了一种bPAG的可视化检测方法,并对适配体浓度、适配体-辣根过氧化物酶浓度、封闭剂浓度、反应时间、包被缓冲液等条件进行了优化.结果显示,在优化条件下,bPAG9在0.5～100 ng/mL浓度范围内呈良好线性(R=0.998),检测限为0.22 ng/mL,目测可视化检测限0.5 ng/mL.采用3个梯度浓度(1,10,100ng/mL)的bPAG9做空白样品添加实验,平均加标回收率为93.2％～105.8％,相对标准偏差为3.51％～7.13％.将建立的方法应用于人工授精30d后的奶牛血清检测,并采用超声波检测法进行对比验证,本方法妊娠诊断准确率达到91.7％.本研究为奶牛早期妊娠诊断提供一种简便、经济、可靠的快速检测新技术.

采用沉淀聚合方法,以齐墩果酸为模板,系统考察了功能单体、溶剂、交联剂及引发剂等对齐墩果酸印迹聚合物(OA-MIPs)形貌以及吸附性能的影响.结果表明,分子模拟计算结果不完全适用于沉淀聚合方法,在沉淀聚合时,溶剂和功能单体对聚合物的形貌起主导作用.在以乙腈+乙醇(二者体积比为3:1)为溶剂,甲基丙烯酸为功能单体,二乙烯基苯+二甲基丙烯酸乙二醇酯(二者的物质量之比为4:1)为交联剂,模板:功能单体:交联剂为1:4:20(物质量之比),偶氮二异丁腈用量为反应单体总量的2％等优化条件下,制备的OA-MIPs形貌规则、粒径均一,对齐墩果酸具有较高吸附性能.结果可为齐墩果酸分子印迹聚合物的制备和应用提供数据参考.