Lonicera macranthoides Hand.-Mazz. is a valuable medicinal plant in China and is used worldwide. This study aimed to predict its suitable habitats in China using the MaxEnt model, and to assess the effects of environmental variables on indicator ingredients (chlorogenic acid, macranthoidin B, and dipsacoside B) via HPLC and chemometrics. Furthermore, to explore the molecular mechanisms underlying environment-quality relationships, preliminary indoor versus outdoor stress experiments were conducted, analyzing the expression of chlorogenic acid biosynthetic genes using qRT-PCR. The results showed that precipitation of the driest month was the most influential variable affecting distribution. Currently, suitable areas are mainly located between 21° N and 33° N. During the Last Glacial Maximum (LGM), habitats were more expansive, whereas they contracted during the Mid-Holocene (MH). Future projections indicated habitat loss under the SSP585 scenario, which was partially mitigated under the SSP126 scenario by 2090 S. Higher contents of chlorogenic acid and saponins were found in suitable habitats and were associated with soil, altitude, and precipitation. Notably, outdoor combined stress (low temperature and low sunshine) significantly regulated the expression of LmPAL, LmCHS, LmCHI, LmC4H, LmCCoAOMT, and LmANS. This study serves as a scientific basis for the conservation, sustainable cultivation, and stress-oriented breeding of L. macranthoides in China.

Photoperiod is a crucial environmental cue that regulates flowering time in plants, playing a vital role in crop adaptability and early maturity. However, the molecular mechanisms underlying photoperiod-regulated flowering in cotton (Gossypium hirsutum L.) remain unclear. In this study, cotton plants were exposed to different photoperiod treatments during the seedling stage. Phenotypic evaluation, transcriptomic sequencing, and metabolomic profiling were integrated to systematically investigate the effects of photoperiod on flowering time and the associated molecular and metabolic regulatory pathways. The results showed that long-day treatments significantly accelerated budding and flowering in cotton, advancing by 20 and 17 days, respectively, compared to short-day conditions. Transcriptome analysis identified numerous differentially expressed genes (DEGs) involved in photoperiod response, hormone signaling, and metabolic regulation. Weighted Gene Co-expression Network Analysis (WGCNA) further revealed that key photoperiod-related genes, including GhFKF1, were upregulated under long-day conditions and formed co-expression networks with flowering regulators. Integrated transcriptomic and metabolomic analyses revealed significant enrichment in glycerophospholipid metabolism, α-linolenic acid metabolism, and flavonoid biosynthesis pathways. Long-day treatment suppressed the expression of key genes and precursors involved in jasmonic acid biosynthesis, while simultaneously upregulating genes involved in flavonoid biosynthesis, leading to increased accumulation of metabolites such as myricetin. Therefore, we propose a theoretical model in which long-day treatment during the seedling stage integrates hormonal and photoperiodic signals by upregulating the expression of the GhFKF1 gene. This regulation may contribute to the initiation of flowering by simultaneously suppressing jasmonic acid biosynthesis and activating the flavonoid biosynthetic pathway. Our findings offer a theoretical foundation and a novel perspective for understanding the photoperiodic response and molecular mechanisms underlying early maturation in cotton.

Glyphosate-resistant crops, developed using the EPSPS gene, are widely cultivated globally. Previous studies indicate that the TIPS-OsEPSPS gene provides glyphosate resistance in rice. In this research, the OsmEPSPS gene was utilized to develop glyphosate-resistant, maize, and soybean, and a rapid immunochromatographic strip (ICS) test was designed for specific detection of the OsmEPSPS protein. The ICS test employs a double-antibody sandwich format with two monoclonal antibodies: mAb #9, conjugated to colloidal gold for capture, and mAb #15, for detection at the test line. The ICS exhibited a limit of detection (LOD) of 0.031 μg/mL within 10 min. Additionally, the ICS could detect a concentration of 0.817 μg/mL within 1 min in both Eleusine indica and OsmEPSPS-overexpressing plants. The ICS is highly specific, sensitive, and stable, with an R2 value of 0.982. Notably, the ICS is capable of detecting EPSPS proteins with high homology to OsmEPSPS from Poaceae and other plants. In summary, this study developed a sensitive and specific test strip for OsmEPSPS detection, facilitating semiquantitative analysis on the basis of colorimetric response time and intensity. This ICS is not only suitable for the rapid identification of OsmEPSPS-overexpressing plants but also for phylogenetic analysis of EPSPS gene homology.

The impact of heavy metals on crop development is mitigated by biochar. Uncertainty surrounds biochar's role in controlling cotton bud stage reactions to Cd-Pb-As combined stress and the alterations in soil microbes that accompany it. Here, we showed that biochar (10 g & sdot;kg-1) successfully reduced the harmful effects of cotton under Cd-Pb-As combined stress. Biochar accelerated bud initiation by two days, increased biomass, and decreased the amounts of Cd (8.58 %) and Pb (15.62 %) the roots absorbed, Pb (32.46 %) and As (26.44 %) by the stems, and Pb (36.19 %) by the leaves. Biochar influenced metal transporter (ABC and ZIP) gene expression, contributing to ion homeostasis. To counteract the adverse effects of Cd-Pb-As combined stress, biochar enhanced photosynthetic efficiency and downregulated genes involved in photosynthesis. Biochar reduces oxidative damage induced by heavy metals by decreasing MDA levels. It also helped cotton maintain cellular and redox balance. This was achieved by regulating antioxidant enzyme activities and HSPs. Additionally, it controlled genes related to lignin synthesis and terpene metabolism through signal transduction pathways. Under Cd-Pb-As combined stress, biochar changed the composition of the rhizosphere bacterial community at the bud stage. It increased the abundance of heavy metal stress-tolerant Proteobacteria, Actinobacteria, Gemmatimonadetes, Nitrospirae, and Chloroflexi while decreasing the abundance of Acidobacteria, Bacteroidetes, Sphingomonas, and Acidobacterium. The findings elucidate the molecular mechanisms by which biochar mitigates Cd-Pb-As combined toxicity in budstage cotton and highlight associated changes in the rhizosphere microbial community.

During the cotton harvesting stage, the application of chemical harvest aids, such as thidiazuron and ethephon, facilitates cotton defoliation and boll maturation, serving as a crucial management tool in modern cotton cultivation systems. This paper reviews recent advancements in cotton defoliation and ripening research; delves into the physiological mechanisms underlying defoliation, boll maturation, and cotton fiber development; and summarizes the effects of major defoliants and herbicide-type desiccants on plants. It also explores the roles of hormones and genes that are involved in the defoliation process and identifies the key factors influencing the effectiveness of harvest aids. Additionally, this paper offers recommendations and scientific prospects for optimizing cotton defoliation and ripening technologies in the future. Through these contributions, it aims to provide valuable insights for the research and application of efficient harvesting of mature cotton, stimulate innovation in cotton defoliation and ripening technologies, enhance the quality and yield of cotton, reduce labor costs, and contribute to the sustainable development of the cotton industry.

The summer-sowing short-season cotton cultivation model is an important method for simplified and mechanized cotton planting in the Yangtze River Basin. However, the effects of microbial fertilizers on cotton growth and soil under this model remain unclear. In 2023, we conducted a systematic analysis on the application of microbial fertilizers (compost) at varying levels (CK, MF1, MF2, and MF3) during different growth stages of cotton (bud, flowering, bolling, and boll opening). Results showed that appropriate microbial fertilizer application (MF2 and MF3) enhanced soil bacterial and fungal diversity, enriched beneficial microorganisms (e.g., Acidobacteriota and Candidatus Udaeobacter), improved soil nutrient availability, and increased antioxidant enzyme activity (POD, SOD), while reducing membrane lipid peroxidation (MDA). These effects led to significant improvements in yield traits, such as cotton plant height, number of fruiting branches and bolls, boll weight, and coat weight. The highest microbial fertilizer application level (MF3) resulted in a 54.35% increase in seed yield and a 75.37% increase in lint yield compared to CK. PLS-DA (Partial Least Squares Discriminant Analysis) and multivariate statistical analyses revealed that microbial fertilizer application fine-tuned soil microbial community composition, emphasizing the dynamic balance of the microbial ecosystem. This study provides scientific support for optimizing microbial fertilizer strategies to enhance the yield and quality of summer-sown short-season cotton and promote sustainable agriculture.

Herbal tea blends are popular because of their unique flavors and health benefits; however, research on their dual aspects of flavor and functionality is limited. The results of the sensory evaluation revealed marked reductions in the green aroma of the blended herbal tea (LBT), accompanied by a pronounced increase in sweetness. The quantitative analysis of key compounds based on Odor activity value and gas chromatography-olfactometry analyses revealed that the content of green aroma compound in LBT (18 %) was significantly lower than the combined values of Longhui Jinyinhua (LJ, 70 %) and Black Tea (BT, 4 %), representing less than half of their total. Conversely, the sweet aroma compound content in LBT (82 %) exceeded half of the combined values of LJ (30 %) and BT (96 %). Quantitative analysis using an electronic tongue revealed a reduction in astringency, with LJ registering 30 %, BT at 12 %, and LBT at 15 %. Ultrahigh-performance liquid chromatography-tandem mass spectrometry further revealed reductions in 11 compounds, including chlorogenic acid, isoquercitrin, and caffeic acid, which were identified as key contributors to mitigating astringency. Additionally, LBT had a greater flavonoid content than did BT, enhancing its antioxidant effects. The findings of this study provide valuable guidance for research on the flavor compounds of herbal tea blends.

为促进金银花多酚组分释放,提高其抗氧化、抗过敏能力,该文利用黑曲霉对金银花进行固体发酵.结果显示,以总多酚、总黄酮含量为参考指标,选择6 d作为最优发酵时间.发酵0～6 d,黑曲霉发酵金银花醇提物DPPH·清除率由61.25％提升至84.57％,·OH清除率由23.43％提升至30.10％,ABTS+·清除率由32.09％提升至55.06％,总还原力由0.730 9提升至0.826 9;透明质酸酶抑制率由13.51％提升至64.86％.总多酚含量由76.60 mg/g提升至97.56 mg/g,总黄酮含量由20.76 mg/g提升至24.12 mg/g,总皂苷含量由3.50 mg/g提升至4.26 mg/g.同时,测得黑曲霉所产纤维素酶、β-葡萄糖苷酶和木聚糖酶酶活的最高值分别为11.70、15.78、32.39 IU/g.该研究表明黑曲霉固态发酵金银花,能够促进金银花中多酚物质释放.

In order to investigate the protective effect of the ethanol extract of Lonicera macranthoides on oxidative stress induced by D-galactose in mouse embryonic fibroblasts（MEF） and its potential mechanism, the active substances in the ethanol extract of Lonicera macranthoides were detected by extensive target metabolome sequencing and high performance liquid chromatography. The MEF cells were treated with 20 mg/mL D-galactose to establish a cell aging model. According to experimental groups, the MEF cells of the control group（0 mg/m L D-galactose）, model group（20mg/mL D-galactose）, Lonicera macranthoides ethanol extract group（100, 500, 1000 μg/m L ethanol extract of Lonicera macranthoides added with 20 mg/mL D-galactose） and positive control group（50 μg/m L Panax notoginseng saponin added with 20 mg/mL D-galactose） had been grew for 48 hours, observed the number of cells with ordinary light microscope, detected the inhibition rate of cells with CCK-8 method, detected the activity of superoxide dismutase（SOD）and the content of malondialdehyde（MDA） with biochemical method, detected the level of mitochondrial membrane potential（MMP） and mitochondrial reactive oxygen species（mtROS） with flow cytometry, and detected the expression of p53, p16. Besides, the expression of mitochondrial complex gene, Bcl-2, Caspase-3, and the expression of mitochondrial complex protein, Bcl-2, Caspase-3 were detected by Western Blot. The results showed that the ethanol extract of Lonicera macranthoides contained phenolic acids, flavonoids, organic acids, terpenoids and other active substances. Compared with the control group, the inhibition rate of cells in the model group was significantly increased andcompared with the model group, the ethanol extract group of Lonicera macranthoides and the positive control group effectively reduced the cell inhibition rate（P＜0.05）, significantly increased the number of cells（P＜0.05, P＜0.01） and SOD activity, decreased the content of MDA（P＜0.05, P＜0.01）, increased MMP, decreased the level of mtROS（P＜0.01）, effectively reduced the protein levels of p53, p16, Caspase-3, and increased the protein levels of Bcl-2 and NDUFV1（P＜0.05）. It showed that in MEF cells, the ethanol extract of Lonicera macranthoides can resist the oxidative stress induced by D-galactose by increasing the concentration of mitochondrial complex I protein and slowing down cell apoptosis.

Light has important effects on plant metabolism. However, the relationship between the chlorogenic acid (CGA) content and light in plants remains unclear. Here, we investigated the effects of shading treatment on gene expression and CGA content in Lonicera macranthoides Hand.-Mazz. (LM), a widely used medicinal plant. A total of 1891 differentially expressed genes (DEGs) were obtained in flower buds and 819 in leaves in response to light in shading treatment compared to the control sample by RNA-Seq. After shading treatment, the content of CGA in LM leaves decreased significantly by 1.78-fold, the carotenoid content increased, and the soluble sugar and starch contents significantly decreased. WGCNA and the expression of related genes verified by qRT‒PCR revealed that CGA synthesis pathway enzyme genes form a co-expression network with genes for carbohydrate synthesis, photosynthesis, light signalling elements, and transcription factor genes (TFs) that affect the accumulation of CGA. Through a virus-induced gene silencing (VIGS) system and CGA assay in Nicotiana benthamiana (NB), we determined that downregulation of NbHY5 expression decreased the CGA content in NB leaves. In this study, we found that light provides energy and material for the accumulation of CGA in LM, and light affects the expression of CGA accumulation-related genes. Our results show that different light intensities have multiple effects on leaves and flower buds in LM and are able to coregulate LmHY5 expression and CGA synthesis.

本文旨在基于网络药理学方法分析金银花抗菌有效成分，预测其分子作用机制。从中药系统药理学数据库与分析平台TCSMP数据库以及文献中收集金银花有效成分及其作用的靶点，通过Uniprot蛋白数据转化为相对应的靶点基因。将有效成分和靶点基因导入Cytoscape3.7.2软件，构建金银花有效成分-靶点网络调控图。通过GeneCards数据库检索并筛选抗菌靶点，与金银花有效成分-靶点取交集得到金银花抗菌核心靶点。将金银花核心靶点基因上传至STRING数据库，构建抗菌靶点蛋白相互作用网络图。最后，通过DAVID数据库将金银花关键靶点进行GO富集分析和KEGG通路富集分析。结果显示，在金银花中共获得23种与抗菌活性有关的潜在化合物，获得了137个抗菌靶点。GO富集分析获得条目17个，其中生物过程10个、分子功能1个、细胞组成6个；它们参与了22条相关信号通路。预测槲皮素、山奈酚、木犀草素、β-谷甾醇和β-胡萝卜素等化合物是金银花抗菌的主要成分。这些化合物作用于MYC原癌基因蛋白（MYC proto-oncogene protein,MYC）、管内皮生长因子A（vascular endothelial growth factor A,VEGFA）、白细胞介素-6（interleukin-6,IL-6）、肿瘤坏死因子（tumor necrosis factor,TNF）等关键靶点，并参与调控TNF信号通路、MAPK信号通路、ErbB信号通路、HIF-1信号通路和Toll样受体信号通路等发挥其抗菌作用。结果表明，金银花具有多成分、多靶点、多通路的抗菌分子机制，为深入阐释金银花及其抗菌分子机制提供理论参考。

为明确陆地棉细胞分裂素受体基因(GhCRE1)在调控植物生长发育中的功能,为研究陆地棉GhCRE1基因调控种子萌发的分子机理提供材料,进一步从分子水平上提高种子萌发率,通过克隆陆地棉GhCRE1基因,利用基因过表达技术将该克隆片段连接至含Ubiquitin启动子的pCUbi1390载体上构建该基因的过表达载体,同时利用CRISPR/Cas9基因编辑技术设计拟南芥CRE1基因的sgRNA片段,合成sgRNA核苷酸序列,从而构建拟南芥CRE1基因靶向敲除载体.将以上2种载体转化农杆菌,使用花序浸染法,以拟南芥为受体材料创建GhCRE1过表达株系及基因编辑功能缺失突变体.接着利用PCR技术和qPCR技术筛选鉴定获得过量表达的GhCRE1拟南芥株系及CRE1功能缺失的拟南芥株系并收获种子,经过对比统计收获的种子与野生型种子在1/2MS培养基上的萌发势进行表型分析,进一步明确陆地棉GhCRE1基因影响种子萌发的功能.结果显示,本研究成功获得了拟南芥GhCRE1过表达株系及CRISPR基因编辑功能缺失突变体株系,且通过数据整理后发现,在种子萌发第2天时,相比野生型,2个过表达转基因株系萌发势分别提高15％和11％左右,而拟南芥CRE1功能缺失型突变体萌发势降低15％左右.该研究揭示,GhCRE1基因过表达能显著提高拟南芥萌发速度.

糖基转移酶Ⅰ(GT Ⅰ)家族是糖基转移酶中的一类,其主要功能涉及黄酮类化合物糖基的转移、淀粉和蔗糖的合成等.为揭示GTⅠ基因家族在粳稻生长发育以及抗逆中的作用,为粳稻GTⅠ基因家族成员的功能研究提供基础,为水稻的品种改良和提高水稻耐高温提供思路,采用生物信息学方法鉴定出30 个GTⅠ基因家族成员,并分析了粳稻GTⅠ基因家族成员的基因结构、蛋白质理化性质、染色体定位及基因进化、系统发育关系、保守结构域和蛋白质三维结构建模等.结果显示,粳稻的30 个GT Ⅰ基因家族成员在水稻12 条染色体上均有分布,其基因结构、保守基序和遗传进化相对保守,家族成员启动子均预测到脱落酸响应元件,家族蛋白质的三维结构保守含有 6 个β-折叠和7 个α-螺旋.通过转录组数据与实时荧光定量分析,GTⅠ基因家族大部分成员对高温胁迫的响应出现在高温处理后第6 小时,其中OS-GT29 表达量呈持续高表达.GTⅠ基因家族在拟南芥和粳稻功能存在相似.

为确定龙牙百合所感染病毒种类,有效减少百合病毒性病害带来的损失,采用随机取样法在湖南省隆回县取染病植株样本9株,以百合斑驳病毒(Lily mottle virus,LMoV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)和百合无症病毒(Lily symptomless virus,LSV)3种百合主要病毒已公布序列设计短引物,采用95℃预变性15 min,95℃变性10 s、60℃退火30 s、72℃延伸30 s,45个循环进行RT-qPCR.扩增结果为:LMoV-99、LMoV-101引物扩增产物的片段长度在100 bp左右,符合预期设计所能扩增出的片段长度,而LSV-169、CMV-70引物扩增产物的片段长度在50 bp以下,不符合预期片段长度,而LSV-70基本没有获得PCR产物.检测结果为:龙牙百合感染病毒为百合斑驳病毒,未感染黄瓜花叶病毒和百合无症病毒.

利用3种叶绿体DNA条形码序列ndhF、psbA-trnH、rps16对竹亚科植物17个属的98个竹种进行DNA条形码聚类分析,并对已知的43个耐盐竹种进行聚类分析.结果显示:3种DNA条形码序列对竹子具有良好的通用性,平均通用性在96％以上;psbA-trnH未能对瓜多竹外的竹亚科植物种属进行聚类,能对部分耐盐竹种进行聚类;ndhF、rps16条形码序列能对部分种属进行聚类,对牡竹属、箣竹属、苦竹属中包含的耐盐竹种聚类效果较好.

为使苎麻生物脱胶果胶裂解酶PelG403高效表达.将pelG403从重组质粒pEASY-Blunt E1-G403更换至质粒拷贝数更高的pET28a载体中,导入Escherichia coli BL21(DE3)后进行诱导表达.采用DNS法进行酶活力测定,通过SDS-PAGE和Western Blot分析检验PelG403表达效果,并对其序列进行分析鉴定.结果表明:pET28a-G403/BL21的果胶裂解酶活力为335.8 U/mL,较pEASY-Blunt E1-G403/BL21提高了3.05倍;工程菌pET28a-G403/BL21表达产物分子量比预测带His标签的融合蛋白分子量(43.6 ku)略小;PelG403含387个氨基酸,N末端前35个氨基酸为信号肽;理论pI值为7.64,有4个半胱氨酸,不稳定系数为27.42;其蛋白结构域含有典型的右手β-螺旋结构,属于Pec lyase C家族.因此,将pelG403从重组质粒pEASY-Blunt E1-G403更换至pET28a载体中,并导入E.coli BL21(DE3)进行诱导表达,是苎麻脱胶果胶裂解酶基因pelG403高效表达的方法和途径,同时对其序列进行分析鉴定,为果胶裂解酶的纯化、关键位点分析、分子改造及其脱胶功能特性研究奠定基础.

Cytokinin plays a very important role in plants growth and development, and CRE1 has been identified as a cytokinin receptor. However, the biological function of CRE1 in cotton remains unclear. In this paper, GhCRE1 gene was cloned and its sequence was analyzed by bioinformatics. The results revealed that GhCRE1 had 11 exons and 10 introns, and its molecular weight, theoretical isoelectric point (pI) and number of amino acids differed from those of the homologous gene in Theobroma cacao and Arabidopsis thaliana, with three different protein domains. 15 unidentified proteins were found in potential interaction with CRE1 protein and 2 phosphorylation sites on CRE1 protein sequence were predicted by mutiple bioinformatics websites. Additionally, 8 cis-acting regulatory elements were detected on CRE1 promoter sequence, and found related to light signal and hormone. These results were conducive to unfolding the function of GhCRE1 in upland cotton.

Fatty acid desaturase-2 (FAD2) is a key enzyme in the production of polyunsaturated fatty acids in plants. RNAi technology can reduce the expression of FAD2 genes in Brassica napus seeds and acquire transgenic B. napus plants with a high oleic acid content, but the effect of seed-specific inhibition of FAD2 expression on B. napus seed metabolites is not clear. Here we use widely targeted metabolomics to investigate the metabolites of normal-oleic-acid rapeseed (OA) and high-oleic-acid rapeseed (HOA) seeds, resulting in a total of 726 metabolites being detected. Among them, 24 differential metabolites were significantly downregulated and 88 differential metabolites were significantly upregulated in HOA rapeseed. In further lipid profile experiments, more lipids in B. napus seeds were accurately quantified. The contents of glycolipids and phospholipids that contain C18:1 increased significantly and C18:2 decreased because FAD2 expression was inhibited. The changes in the expression of key genes in related pathways were also consistent with the changes in metabolites. The insertion site of the ihpRNA plant expression vector was reconfirmed through genomewide resequencing, and the transgenic event did not change the sequence of FAD2 genes. There was no significant difference in the germination rate and germination potential between OA and HOA rapeseed seeds because the seedspecific ihpRNA plant expression vector did not affect other stages of plant growth. This work provides a theoretical and practical guidance for subsequent molecular breeding of high OA B. napus.

本文以具有抗二化螟性状的Csu-miR260转基因水稻为试验材料,采用TaqMan探针实时荧光定量PCR(TaqMan RT-qPCR)和茎环实时荧光定量PCR(stem-loop RT-qPCR)分别对Csu-miR260转基因抗虫水稻中Csu-miR260插入序列和剪切形成的amiR260s的表达情况进行了定量检测.发现Csu-miR260插入序列和剪切形成的20 nt和21 nt两种长度amiR260s在转基因抗虫水稻苗期的根、茎、叶中表达,且无组织表达特异性.该试验解决了人工miRNA作物中amiRNA及前体检测引物的特异性问题,为人工miRNA植物干扰序列的表达分析提供技术参考,并为miRNA转基因作物遗传表达相关安全评价提供了数据参考.

利用SSR分子标记建立芥菜品种鉴定体系,旨在为我国芥菜育成品种鉴定提供高通量、快速鉴定方法.选取芥菜16个变种代表性品种用于SSR引物初筛,通过聚丙烯酰胺凝胶电泳从432对引物中筛选出84对条带清晰、稳定性好、多态性高的芥菜SSR引物,并对筛选出的引物5'端进行荧光标记.另外选取96份品种对上述84对引物进行复筛,利用基因分析仪对所有扩增片段大小进行分析,依据各引物的扩增稳定性、峰型易读取程度、多态性及染色体位置等,最终筛选出25对SSR引物作为核心引物,并为其设置参照品种以消除不同试验批次或平台等因素引起的误差.根据荧光颜色和扩增片段大小将25对核心引物分成6组,建立基于SSR标记的芥菜品种鉴定体系.利用该套核心引物构建了 189份芥菜品种的DNA指纹数据库,共检测到175个等位变异,平均每对引物检测到7个等位变异;基因多样性指数变化为0.239～0.870,平均为0.555;多态性信息含量在0.23～0.86之间,平均为0.493.本研究建立的基于毛细管电泳平台的芥菜SSR分子标记品种鉴定体系,可用于芥菜品种真实性快速鉴定、遗传多样性分析和DUS测试近似品种辅助筛选等.