Objective To assess the efficacy of cognitive behavioral therapy on the symptoms , quality of life and erectile function of chronic prostatitis (CP) .Methods The 148 cases of CP patients were divided into control and experimental groups by stratified random in accordance with NIH -CPSI score .The control group employed conventional treatment for CP ,while the experimental groups com‐bined cognitive behavioral therapy with conventional treatment ,which was as same as the control group . After 4 weeks ,statistical analyses were used to evaluate the treatment effect of CP in both groups by comparing NIH -CPSI score ,CF-12 score and IIEF-5 score before and after treatment .Results Com‐paring with the control group , the NIH -CPSI score was statistically improved in the experimental group (P < 0 .01) .After treatment ,the PCS and MCS scores in the experimental group were higher than that in control group (P< 0 .05) .There was no statistically significant difference in IIEF -5 score before and after treatment in both groups (P> 0 .05) .Conclusions Cognitive behavioral therapy can im‐prove symptoms and quality of life of patients suffering from CP ,but cannot improve the erectile func‐tion .

Objective To investigate the impact of service model by both oncology specialists in tumor and community doctors on the quality of life for survivors with colorectal cancer.Methods Sixty one survivors with colorectal cancer were divided as trial group(n=31) and control group(n=31).The patients in the trial group were given intervention and follow-up by community doctors under guidance of oncology specialists or medical institutions-community service model.And the patients in the control group were no intervention.The Quality of Life Questionnaire-C30(QLQ-C30) in European Organization for Research and Treatment of Cancer(EORTC) was used to evaluate the quality of life of the patients in both groups at baseline and 3 months after the intervention.Results After 3 months,the differences between the two groups in scores of emotion capacity((67.3 ± 12.5) vs.(56.7 ± 13.2)),general health((81.4±6.9) vs.(72.4±6.4)),insomnia((23.7±16.9) vs.(50.3±30.1)),constipation((20.8±11.7) vs.(36.3±17.2)),pain((0.9±0.7) vs.(1.9±1.7)) were statistically significant(P0.05).Conclusion Under the medical institutions-community service model,the quality of life of colorectal cancer survivors can be effectively improved.

Objective To observe the efficacy and toxicities of vinorelbine or paclitaxel combined with cisplatin in the treatment of advanced breast cancer.Methods Two hundred patients with advanced breast cancer were divided into 2 groups,the 100 patients in the NP regimen group received the vinorelbine plus cisplatin chemotherapy(vinorelbine 50 mg·d-1,d1,8;cisplatin 30 mg·m-2,d1-5),the 100 patients in the TP regimen group received paclitaxel plus cisplatin chemotherapy(paclitaxel 110-140 mg·m-2,d2;cisplatin 30 mg·m-2,d1-5).Results The response rate was 61% of the NP regimen group,and was 55% of the TP regimen group(P>0.05).The survival time was 7.5 months of the NP regimen group,and was 7.2 months of the TP regimen group(P>0.05).Conclusion NP and TP regimens have similar efficacies for advanced breast cancer.

目的 探讨心理干预对恶性肿瘤患者焦虑抑郁状态及生活质量的影响.方法 将205例恶性肿瘤患者随机分为对照组和心理干预组,对照组采用常规放疗、化疗及最佳支持治疗,心理干预组在常规治疗基础上对患者进行心理干预,治疗前后采用焦虑自评量表(SAS),抑郁自评量表(SDS)和EORTC-QLQ-C30量表对患者进行焦虑抑郁状态及生活质量的测定.结果 两组患者入院时SDS及SAS评分比较无统计学差异(P＞0.05),心理干预组第6周SDS及SAS评分均明显低于对照组,差异有统计学意义(P＜0.05),心理干预后可改善癌症患者生活质量,较干预前差异有统计学意义(P＜0.05).结论 心理干预能够改善恶性肿瘤患者焦虑抑郁情绪,提高生活质量,有利于患者的康复.

目的提高对恶性肿瘤患者合并血栓栓塞性疾病的认识。方法对46例合并血栓栓塞性疾病的恶性肿瘤患者进行回顾性分析。结果本组脑恶性肿瘤6例,占13.0%;前列腺癌5例,占10.9%;肺癌7例,占15.2%;大肠癌3例,占6.5%;胃癌4例,占8.7%;卵巢癌3例,占6.5%;子宫颈癌4例,占8.7%;乳腺癌3例,占6.5%;肾癌2例,占4.4%;胰腺癌7例,占15.2%;非霍奇金淋巴瘤2例,占4.4%;深静脉血栓31例,占67.4%;脑血栓7例,占15.2%;D IC 4例,占8.7%;PE 3例,占6.5%;PVT 1例,占2.2%。病理类型主要为腺癌,27例占58.7%。结论恶性肿瘤患者可伴发血栓栓塞性疾病,可能与血液的高凝状态、肿瘤化疗和中心静脉导管植入有关。

目的:观察紫杉醇单药治疗老年晚期非小细胞肺癌(NSCLC)的临床疗效。方法:对36例70～80岁NSCLC患者应用国产紫杉醇60mg/m2,静脉滴注,第1、8、15天,28d为1周期,至少治疗2周期。结果:共完成122周期,总有效率(CR+PR)25.0%(9/36),临床受益率(CR+PR+SD)58.3%(21/36)。毒副作用以白细胞和血小板下降为常见,均可耐受。结论:紫杉醇单药治疗老年晚期NSCLC可改善患者症状和提高生活质量。

Objective:Choosing α-MSH as the targeting part and Ang as the toxic part,a specificity recombinant toxin using the genes of α-MSH and Ang was constructed.Methods:Gene recombinant technique was used to combine α-MSH gene with toxin Ang gene and cq cloned into PET-20b plasmid.Construct recombination protein in PCR method.Results:pET/MSH-Ang could be constructed by α-MSH gene and Ang gene.Conclusion:Constructing of expression plasmid was right by restriction endonucleases and DNA sequencing.

Objective To explore the expression condition,and zymotechnics condition and purification system of recombinant protein.Methods The recombinant toxin MSH-Ang was constructed by chemical couple and gene fusion system.Results Recombination protein exist in E.Coli BL21 in soluble form;an expressive condition was required.Conclusion The expressive rate can be elevated by chromatography and purification.

目的:观察紫杉醇与顺铂联合化疗对晚期卵巢上皮性癌近期疗效及毒副反应。方法:晚期卵巢上皮性癌36例,采用PT案化疗,紫杉醇135 mg/m2,顺铂70 mg/m2全身化疗,3周为1疗程,至少完成4个疗程观察疗效。结果:Ⅲ期有效率为71.42%;Ⅳ期有效率为50.00%。毒副反应主要为骨髓抑制,发生率72.22%(26/36),但61.11%(22/36)均为Ⅰ~Ⅱ度。胃肠道反应发生率55.56%(20/36),所有患者均未因毒副作用中断或退出治疗。结论:PT方案联合化疗效果较好,毒副反应小。

原发性肝癌是我国常见的恶性肿瘤之一,恶性程度高,起病隐匿,复发率高,能够接受手术切除治疗的肝癌患者仅占20%左右[1].故临床上大多数肝癌仍需采用非手术疗法.肝癌主要治疗手段有手术、放疗、化疗、介入治疗等.对于病变范围小、孤立、数目少的可以手术或放疗;对于病变大又有包膜的可以介入栓塞治疗.但原发性肝癌往往当发现时,已是晚期,病变较大或弥散,手术、介入栓塞难以起作用.晚期肝癌多数伴有不同程度的肝硬化及肝功失代偿,化疗效果不佳.不断探索和研究新的治疗方法,显得十分重要.随着高热治疗癌的进展,大量的临床实践及生物实验表明热疗联合化疗有明显的互补与增敏效果.我科自2003年11月～2004年11月采用局部热疗联合化疗治疗63例晚期原发性肝癌患者,取得满意疗效,现报告如下:

Objective To observe the clinical efficacy of OLF (oxaliplatin plus CF and 5-Fu) regimen and LFP (DDP plus CF and 5-Fu) regimen in treatment of the patients with advanced gastric carcinoma.Methods Forty-eight patients with advanced gastric carcinoma received chemotherapy with OLF regimen(OLF group,26 cases)and LFP regimen(LFP group,22cases)respectively.The efficacy and the adverse reaction of the two regimens were compared.Results The effective rate was 53.8% in OLF group and 45.5% in LFP group.Median survival time was 9.5 months and 6.5 months for OLF group and LFP group respectively. The main adverse reaction was suppression of bone marrow with reduction of WBC,with no significant difference between the two groups (P0.05). The reaction of gastrointestinal tract was nausea,vomiting and anorexia,with significant difference between the two groups (P0.01).Conclusion The two regimens OLF and LFP have good efficacy in treatment of the patients with advanced gastric carcinoma. The effective rate was not significanly different between the two groups. However, the OLF regimen(oxaliplatin plus LV and 5-Fu) is effective and safe and the patients receiving it had a better quality of life and increased survival rate.It is deserved to be spread.

Objective To study the repairing effect of NADH on PC12 cells damaged by Aβ_ 25-35 . Methods We determined the cytotoxicity of Aβ_ 25-35 on PC12 cells with cell proliferation test and MTT assay. At the same time, we analyzed the cyclin protein, GTPase binding protein and transglutaminaseⅡ content with flow cytometry(FCM). Results NADH significantly inhibited the cytotoxicity of Aβ_ 25-35 on PC12 cells. Aβ_ 25-35 up-regulated the expression of cyclin D1 and down-regulated the expression of cyclin A and B1, while NADH did just the contrary. Still, NADH could up-regulated the expression of GTPase binding protein and transglutaminaseⅡ, which were decreased by Aβ_ 25-35 in PC12 cells. Conclusion NADH can inhibited the cytotoxicity of Aβ_ 25-35 on PC12 cells, which is associated with the regulation of cyclin proteins, GTPase binding protein and transglutaminaseⅡ.

目的 探讨Aβ2 5-3 5致PC12细胞损伤后线粒体COX活性和ATP合成的改变及人参皂甙对它们的影响。方法 Aβ2 5-3 5损伤PC12细胞 ,人参皂甙处理前后紫外分光光度计测定线粒体COX活性 ,高效液相色谱仪测定线粒体能荷。结果 Aβ2 5-3 5损伤PC12细胞后 ,线粒体COX活性降低 ,线粒体ATP、ADP含量降低 ,人参皂甙能提高线粒体COX活性及ATP、ADP含量。结论 人参皂甙通过抗氧化作用可以减轻Aβ2 5-3 5致PC12细胞线粒体的氧化损伤。

目的 比较全身化疗加局部热疗及单纯化疗治疗中晚期非小细胞肺癌的疗效和副反应差异.方法 47例中晚期非小细胞肺癌病人随机分为两组.A组(化疗热疗组)23例,B组(单纯化疗组)24例,热疗化疗组采用局部热疗联合NP方案化疗,单纯化疗组单纯采用NP方案化疗,对比分析两组疗效及毒副反应.结果 A组总有效率52.2%,B组总有效率45.8%.两组有显著性的差异(P＜0.05).两组毒副反应发生率无显著性差异.局部热疗联合化疗不失为治疗中晚期非小细胞肺癌的有效综合治疗方法.结论局部热疗联合全身化疗可提高单纯化疗治疗中晚期非小细胞肺癌的疗效,副作用增加不明显.

中药苦参始载于<神农本草经>,民间有用于治疗癌症,实验与临床证明复方苦参(岩舒)有一定抗肿瘤及提高免疫功能的作用.在放、化疗并用复方苦参的病例中显示出该药有较好的协同效应,能提高肿瘤治疗疗效[1～4] ,自2000年以来,我们用岩舒注射液(复方苦参)治疗老年晚期癌症103例,并与75例化疗进行对比观察,现将结果报告如下.

Objective To explore the relationship between expression of P53 protein in laryngeal carcinoma tissue and microvessedensity(MVD).Methods The positive expression rate of P53 protein and microvessedensity(MVD) were detected by immunohistochemical staining.Results The positive expression rate of P53 protein was 47.62%,and MVD was 35.79±9.49.Both the P53 positive expresion rate and MVD in the tumors metastasized to lymph nodes were significantly higher than those in unmetastasized tumors.Compared with the tumors metastasized to the lymph nodes at cervical part,more P53 protein positive specimens showed high MVD values.Conclusion The expression of P53 gene mutant might have synergistic effect on the formation of capillary vessels of tumor and metastasis of laryngeal carcinoma to the lymph nodes at cervical part.

目的观察托吡酯(Topiramate)添加对难治性癫痫的临床效果与安全性.方法 38例难治性癫痫在不改变原用抗癫痫药(AEDs)的基础上加用TPM治疗,自25mg/d开始,逐渐加量,目标剂量为200mg/d.治疗前及治疗结束后全面观察疗效,同时观察副作用、脑电图改变、肝肾功能等.结果临床疗效总有效率78.9%,38例中控制10例,显疗效12例,有效8例,不变6例,加重2例,其中复杂部分发作有效率最高为83.3%,有较明显的脑电图改善效果,无明显的毒副作用.结论 TPM是目前治疗IE重要的安全有效的选择药物之一.

本文采用回顾性分析方法对我院2000年6月～2003年6月间收治的19例脑膜癌病患者的临床表现及诊断加以分析,加深对脑脊液细胞学检查的重视.