目的 探讨阴道骶骨固定术对女性尿动力和盆底肌电生理的影响.方法 50例盆腔器官脱垂患者,根据手术方式分为观察组30例和对照组20例,观察组采用阴道骶骨固定术进行治疗,对照组采用传统手术进行治疗.2组手术前、后采用盆腔器官脱垂的定量评分评估解剖复位情况,比较手术前、后尿动力学指标腹压漏尿点压、最大尿流率时逼尿肌压、最大尿道闭合压、膀胱顺应性,比较手术前及手术后3、6、12个月盆底肌电生理变化情况.结果 观察组盆腔器官脱垂的定量评分Aa、Ba、C、Ap、Bp点手术前后差值[(5.01±0.42)、(7.73±0.56)、(12.79±0.41)、(4.60±0.31)、(5.09±0.67)cm]均大于对照组[(3.42±0.39)、(6.13±0.53)、(10.27±0.35)、(3.34±0.27)、(3.62±0.55)cm](P＜0.05);2组术前腹压漏尿点压、最大尿流率时逼尿肌压、最大尿道闭合压及膀胱顺应性比较差异均无统计学意义(P＞0.05);观察组术后腹压漏尿点压[(15.23±2.77)kPa]、最大尿流率时逼尿肌压[(10.01±1.57)kPa]、最大尿道闭合压[(9.77±1.54)kPa]、膀胱顺应性[(410.73±123.42) mL/kPa]均高于对照组[(9.34±2.31)kPa、(5.23±1.65)kPa、(6.50±1.72) kPa、(337.52±120.48) mL/kPa] (P＜0.05);观察组术后3、6、12个月Aa点[(-2.85±0.34)、(-2.24±0.31)、(-1.24±0.27)cm]和C点[(-6.82±0.39)、(-4.88±0.37)、(-3.49±0.42)cm]水平均低于对照组[(-1.02±0.52)、(-0.94±0.34)、(-0.85±0.22)cm,(-4.12±0.41)、(-3.75±0.36)、(-3.01±0.37)cm] (P＜0.05),Ⅰ类肌电位和Ⅱ类肌电位水平与对照组比较差异无统计学意义(P＞0.05).结论 阴道骶骨固定术可明显改善女性尿动力.

目的 探讨槲皮素(Que)对紧密连接蛋白6(CLDN6)的表达及子宫内膜异位症细胞增殖、迁移和侵袭的影响,并初步分析其可能作用机制.方法 选取40例子宫内膜异位症患者为研究对象,活检时切取异位子宫内膜组织,分离与培养异位子宫内膜间质细胞(EcSCs).以不同浓度(0、12.5、25、50 μmol/L) Que干预EcSCs,通过MTT法检测不同浓度Que对EcSCs细胞增殖的影响,Western blot检测增殖细胞核抗原(PCNA)与CLDN6蛋白表达.应用Tran-swell迁移及侵袭实验检测细胞迁移及侵袭情况.EcSCs中转染阴性对照(si-control)、si-CLDN6,并分析沉默CLDN6表达对EcSCs细胞增殖、迁移和侵袭的影响.后续实验分为4组:NC组、Que组、Que+ pcDNA组、Que+ pcDNA-CLDN6组,MTT与Transwell迁移及侵袭实验分别检测各组EcSCs增殖、迁移及侵袭能力,Western blot法检测PCNA、基质金属蛋白酶-2(MMP-2)、MMP-9、上皮-间质转化(EMT)及整合素连接激酶(ILK)信号通路相关蛋白表达.结果 Que干预后EcSCs增殖、迁移及侵袭能力受到抑制,且呈剂量依赖性,可抑制CLDN6、PCNA、MMP-2、MMP-9蛋白表达;沉默CLDN6表达后能够抑制EcSCs增殖、迁移及侵袭能力;Que可抑制神经型钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)、ILK、磷酸化蛋白激酶B(p-AKT)蛋白表达,促进E-cadherin蛋白表达,但转染CLDN6过表达载体后可逆转Que对EcSCs增殖、迁移及侵袭的抑制作用.结论 Que可通过抑制CLDN6表达而抑制子宫内膜异位症细胞增殖、迁移及侵袭,其可能作用机制为抑制ILK信号通路活化及EMT转换进程.

Objective To investigate the effect of combination of histone deacetylase inhibitor (HDACI) Suberoylanilide Hydroxamic Acid (SAHA) combined with human papilloma virus 16 E7-DNA (HPV16E7-DNA) vaccine in the treatment of cervical cancer and its immunological mechanism.Methods The model of mice with TC-1 cervical cancer was made,then on the 5th day after tumor bearing,the mice were randomly divided into HPV16E7-DNA group,SAHA group,SAHA and HPV16E7-DNA group,control group by random number table method.The therapeutic regimen was as followed:HPV16E7-DNA group was given vaccine 5 μg/kg,SAHA group was given SAHA 20 mg/kg,SAHA and HPV16E7-DNA group was given vaccine 5 μg/kg+SAHA 20 mg/kg,the control group was given 100 μL phosphate solutions (PBS).Once every 3 days,total for 4 times.The tumor growth curve was drawn;the amount of CD8+T cells in peripheral blood of mice in each group was detected by flow cytometry;the effects of different doses of SAHA (25.0,12.5 nmol/L) for the expression of H-2Kb of MHC class Ⅰ in TC-1 cells were detected by experiment in vitro.Results Compared with the other three groups,the tumor growth rate in SAHA and HPV16E7-DNA group was significantly slowed down,the amount of HPVE7 specific CD8+T cells in peripheral blood of mice was significantly increased (P ＜0.05).In-vitro experiment showed that compared with control group,the expression of H-2Kb of MHC class Ⅰ in SAHA group was increased significantly (P ＜ 0.05),while there was no significant difference between low dose of SAHA group and high dose of SAHA group (P ＞ 0.05).Conclusion HDACI combined with HPV16E7-DNA vaccine comes into significant anti-tumor effects may be through up-regulating expression of MHC class Ⅰ of the tumor cells and activating the immune response of CD8+T cells,which is expected to be used in the immunotherapy of cervical cancer.

[Purpose] To investigate the effectiveness of HPV16E7-HSP90 DNA vaccine in the treatment of cervical cancer in mice and its immunological mechanism.[Methods] On d5 after challenge of mouse cervical cancer TC-1 cells,mice were divided into two groups:HPV16E7-HSP90 DNA vaccine was intramuscularly injected to tumor-bearing mice in DNA vaccine group (5μ g/mice,q3d ×4),while PBS was injected to mice in control group (100μl/mice,q3d ×4).The tumor volume was measured every 3d after tumor challenge and the tumor growth curve was drawn.The expression of CD8+ T cells in tumor tissue and FAS protein in tumor cells were detected by immunohistochemical method at d8 after the completion of treatment.At the same time,the rate of apoptotic tumor cells was detected by TUNEL method.[Results] The tumor growth rate of the mice in DNA vaccine group was consistently lower than that in the control group (P＜0.05).The expression of CD8+ T cells in the tumor tissues and the expression of FAS in the tumor cells in the DNA vaccine group were significantly higher than those in the control group [(8.76±1.27) vs (5.70±1.40);(8.73±-0.99) vs (5.74+1.47);P＜0.05].The expressions of CD8+T cells in tumor tissues was positively correlated with the expression of FAS in tumor cells (P＜0.01).Meanwhile,the rate of apoptotic tumor cells in DNA vaccine group was significantly higher than that in the control group (57.59％ vs 13.11％,P＜0.05).[Conclusion] HPV16E7-HSP90 DNA vaccine shows a significant anti-tumor effect in tumor-bearing mice,which is associated with up-regulating of the expression of FAS,inducing the apoptosis of the tumor cells and improving the tumor specific immune responses of CD8+T cells.

目的 探讨钙网蛋白(calreticulin,CRT)在上皮性卵巢癌中的表达及临床意义.方法 采用免疫组化法检测40例上皮性卵巢癌、20例卵巢良性肿瘤及10例正常卵巢组织中CRT的表达情况.结果 CRT在上皮性卵巢癌中表达明显高于卵巢良性肿瘤及正常卵巢组织,CRT在上皮性卵巢癌中的表达与组织病理分级、临床分期、淋巴结转移均有关,与组织学分型无关.结论 CRT表达升高在上皮性卵巢癌的发生、分化、进展过程中起重要的作用,CRT有可能成为早期诊断的新标志物及治疗卵巢癌的新靶点.

Objective To investigate the clinical value of combined detection of glycoprotein 125 (CA125) and human epididymis protein 4 (HE4).Methods 46 patients with ovarian malignant tumor (malignant tumor group) and 48 patients with benign ovarian tumors (benign tumor group) treated in our hospital from June 2013 to August 2015 were selected.The serum levels of CA125 and HE4 were detected in all the patients and its diagnostic value was evaluated by ROC curve.The levels of CA125 and HE4 in patients with different pathological types were compared.Results The best diagnostic value of CA125 was 47.9 U/L,The serum level of CA125 ≥47.9 U/L predicted the specificity of ovarian malignant tumor was 87.34% and that the sensitivity was 76.69%.The best diagnostic value of HE4 was 55.68 pmol/L.The serum level of HE4 ≥ 55.68 pmol/L predicted the specificity of ovarian malignant tumor was 90.34% and that the sensitivity was 83.01%.There was significant difference in CA125 and HE4 between the patients with benign and malignant ovarian tumors (P<0.05).No significant difference in the diagnosis of malignant ovarian tumor and the specificity by using combined detection of HE4 and CA125 (P>0.05)However,the sensitivity was significantly higher than that of single detection,the difference was statistically significant (P<0.05).The levels of CA125 and HE4 in patients with epithelial ovarian tumors were higher than those with non epithelial ovarian tumors,and the difference was statistically significant (P<0.05).The levels of CA125 and HE4 in patients with mucinous ovarian cancer were significantly lower than those in patients with serous ovarian cancer (P<0.05).Conclusion The combined detection of serum CA125 and HE4 can significantly improve the value of differential diagnosis of ovarian tumors,and CA125 and HE4 may play an important role in the pathological classification of malignant ovarian tumors.

Objective To explore the expressions of regulatory T cells in cervical squamous intraepithelial lesions (CSIL) and cervical carcinoma in young women and the correlations with different degrees of cervical lesions,research the predictive function for prognosis of CSIL.Methods A total of 110 cervical carcinoma patients less than 35 years old were selected from the hospital from June 2014 to June 2016,then they were divided into low-grade squamous intraepithelial lesion (LSIL) group (30 patients),high-grade squamous intraepithelial lesion (HSIL) group (30 patients),and cervical carcinoma group (50 patients).Thirty patients with normal cervix after total hysterectomy due to other diseases (uterine fibroids,endometriosis) during the same period were selected as control group.Immunohistochemical staining was used to detect the expression of forkhead/winged helix transcription factor 3 (FOXP3) in cervical tissues.Results The positive expression rates of FOXP3 in control group,LSIL group,HSIL group,and cervical carcinoma group were 3.3％,36.6％,53.3％,and 100.0％,respectively,there were significant difference,the severer the lesions were,the higher the total positive expression rate was.The positive expression intensity of FOXP3 was correlated with the grades of lesions in control group,LSIL group,HSIL group,and cervical carcinoma group (P＜0.001).Conclusion The positive expression intensity of FOXP3 was correlated with the grades of lesions in young women with CSIL and cervical carcinoma,which can help clinical evaluation of biological behaviors of cervical tumors.

Objective:To study the relationship between the gene polymorphism of methylene tetrahydrofolate reductase (MTHFR) and the unexplained recurrent spontaneous abortion (URSA).Methods:83 patients with unexplained recurrent spontaneous abortion (the URSA group) and 90 normal women of reproductive age (the control group) were recruited.Fluorescence quantitative PCR was used to detect the MTHFR gene C677T,A1298C single nucleotide polymorphisms (SNP).The frequency distribution of genotype and allele was compared,and the risk of different genotypes to URSA was evaluated.Results:The distribution of MTHFR genotypes in both groups was consistent with the Hardy-Weinberg balance.There were significant differences in the frequencies of C677T genotype and alleles between the URSA group and the control group (P＜0.05).There were no significant differences in the frequencies of A1298C genotype and alleles between the two groups (P＞0.05).The frequency of homozygous genotype in the URSA group was higher than that in the control group (30.1％ vs.13.3％,x2=7.237,P=0.007).The risk of URSA in those patients with TT homozygous genotype was 3.289 times higher than that in the patients with CC homozygous genotype (OR=3.289,95％CI:1.342-8.063).Conclusions:The gene polymorphism of MTHFR C677T site is a risk factor of URSA,and the TT homozygoous genotype increases the risk of URSA.

Objective To study the effect of T regulation cells ( Treg cells) on the prognosis of cervical intraepithelial neoplasia grade I(CIN I)patients.Methods 98 cases of CIN I patients admitted to our hospital during March 2013 and May 2014 were enrolled in the study .After 2 years of follow-up, the expression of Treg cells and the development of the patient's condition before and after follow-up were observed .Results The patients were followed up for 2 years.The positive rate of the expression of Treg cells in patients in improvement group was 100%(4/4), it was 47.83%(11/23)and 4.23%(3/71) in the diseases continuous group and upturn group.The differences among groups were statistically significant ( P<0.05) .The Treg cells expressed highest in the improvement group ( P>0.05 ) .Conclusions Treg cells may play an important role in the progression of cervical intraepithelial neoplasia , and play a role in the process of carcinogenesis .

Objective To explore the clinical effect of Foley balloon and absorbable adhesion barrier(Interceed) on the prevention of the relapse of intrauterine adhesions after hysteroscopic operation among patients with severe intrauterine adhesions.Methods Eighty cases of severe intrauterine adhesions in the Affiliated Shenzhen Maternity and Child Healthcare Hospital of the South Medical University were selected as study subjects,and they were divided into three groups.All the patients were treated with estradiol valerate for 3 months after transcervical resection of adhesions(TCRA).Patients of group A (34 cases) were treated with installed intrauterine device(IUD).Patients of group B(23 cases) were treated with Foley balloon and hyaluronic acid,and after 3 days,Foley balloon was taken out and hyaluronic acid was given again,then IUD installed.In the 1st and 2nd month 3 ～ 5 days after menstruation,take out IUD,install Foley balloon,and after 3 days,take out Foley balloon,then install IUD.Group C (23 cases) were treated with Interceed and IUD.After 3 months,hysteroscopy was performed again to evaluate the treatment effect.Results The total effective rate of group B was 87％,and reached 96％ in Group C,which was remarkably higher than that of group A(62％),the difference being statistically significant (P＜ 0.05).Although,the total effective rate of group C was higher than that of group B,there was no significant difference between them (P＞0.05).Conclusion The Interceed anti-adhesion membranes and Foley balloon are all good for preventing intrauterine adhesions after TCRA.

目的:探讨P16InK4a蛋白及Ki-67在年轻妇女宫颈鳞状上皮内病变(CSIL)、宫颈癌组织中的表达及其与不同病变级别的相关性,预测CSIL转归作用.方法:选择2012年1月～2014年12月在广东省妇幼保健院就诊,年龄＜35岁、经病理确诊的宫颈低级别鳞状上皮内病变(LSIL)、宫颈高级别鳞状上皮内病变(HSIL)、宫颈癌患者111例,采用免疫组织化学染色,检测宫颈组织中P16InK4a及Ki-67的表达.结果:①不同级别CSIL及宫颈癌病变组织中,P16InK4a及Ki-67的阳性表达强度与病变级别相关(P＜0.001);②P16InK4a在LSIL、HSIL及宫颈癌的阳性表达率分别为24％、81％、95％,Ki-67阳性表达率分别为37％％,87％,95％,P161nK4a及Ki-67在不同级别CSIL、宫颈癌的总阳性率有统计学差别(P＜0.001).结论:P16InK4a及Ki-67在年轻妇女CSIL、宫颈癌组织中阳性表达强度与病变级别相关,可辅助临床评价肿瘤生物学行为及预测预后.

目的比较体外受精胚胎移植（IVF-ET）后输卵管妊娠和自然受孕输卵管妊娠患者基质细胞衍生因子-1（SDF-1）及CXC趋化因子受体4（CXCR4）mRNA表达的差异,以探讨其在IVF-ET后输卵管妊娠发生中所起的作用及机制。方法分别选取2011年1月至2012年12月10例IVF-ET后输卵管妊娠和10例自然受孕输卵管妊娠患者,在腹腔镜手术的同时抽吸蜕膜、绒毛和输卵管黏膜组织,-70℃冷冻备存;采用荧光定量PCR的方法检测各组织中SDF-1及CXCR4 mRNA表达。结果两组患者胎盘绒毛、输卵管黏膜及子宫蜕膜组织的SDF-1及CXCR4mRNA表达量比较差异均无统计学意义（P均＞0.05）;两组绒毛组织和输卵管黏膜组织均高表达CXCR4与SDF-1mRNA,其表达量显著高于各自的蜕膜组织（P均＜0.05）。显示出受孕方式与输卵管妊娠发生率可能无关。结论输卵管黏膜SDF-1和CXCR4表达亢进,子宫蜕膜SDF-1和CXCR4相对表达不足可能是IVF-ET后胚胎定植于输卵管的重要机制之一。

Objective:To investigate diagnosis and therapy of placenta accrete of the second trimester. Methods:The clinical data of 30 cases of placenta accrete in second trimester of pregnancy in the Affiliated Shenzhen Maternity and Child Healthcare Hospital of the South Medical University was analysed retrospectively from 2005 to 2015. Patients of group A(22 ca-ses):the largest diameter of placenta accreted<5cm,Patients of group B(8 cases):the largest diameter of placenta accreted≥5cm. Results:Only 1 case (3%) was confirmed dignosis with magnetic resonance imaging in antepartum,29 cases were confirmed dignosis after delivered. 29 cases were treated with conservative surgery,1 patient after uterine arterial embolization ( UAE) was performed hysterectomy because of severe intraoperative hemorrhage and postoperative in-fection. Patients of group A,the amount of bleeding was less than 1000ml in 21 cases and more than 1000ml in 1 case,in conservative operation 7 cases were preferred treated with UAE,9 ca-ses were treated with hysteroscopy. Patients of group B, the amount of bleeding was less than 1000ml in 3 cases and more than 1000ml in 5 case,in conservative operation 8 cases were pre-ferred treated with UAE,0 case was treated with hysteroscopy. The difference was statistically significant ( P<0 . 05 ) . Patients of group A:placenta accrete was located on the anterior uterine wall in 16 cases,on the posterior wall in 5 cases,on the fundus uteri in 1 case;Patients of group B:placenta accrete was located on the anterior uterine wall in 5 cases,on the posterior wall in 3 cases. There was no significant difference between them (P>0. 05). Conclusions:Placenta ac-creta in second trimester of pregnancy could lead to severe complications with hysteretic diagno-sis.Ⅰndividuals with risk factors for placenta accreta should be investigated early in pregnancy. Bleeding volume and area of placenta could serves as basis for the selection of treatment op-tions. Conservative methods were preferred and intrauterine infection should be attach impor-tance in the treatment of placenta accreta in second trimester of pregnancy.

Objective To analyze the effect of the heating carbon dioxide pneumoperitoneum on blood gas of patients with gynecological laparoscopic surgery and to explore measures of anesthetic intervention. Methods A total of 1 20 cases of gynecological patients who received laparoscopicoperation therapy in our hospital from May 201 2 to May 201 4 were included and were randomly divided into group A and group B with 60 cases in each group.The patients in group A were treated with the heated carbon dioxide while patients in group B were treated with pneumoperitoneum of non-heated carbon dioxide,Changes of blood gas of the patients in the two groups were compared before and after operation. Results The differences in pH,PCO2 ,PO2 and HCO3 were significant before the surgery.After the surgery,the PO2 value in group A was (21 5.60 ±1 5.20)mmHg,which was significantly lower than (31 2.92 ±23.78)mmHg in group B.The PCO2 value in group A was (1 5.1 1 ±0.22)mmHg,which lower than that in group B.The BE value in group A was (-1 0.22 ± 1 .94)mmol/L after the surgery,which was lower than that in group B. Conclusion Heating treatment is the key factor to affect the operation safety of the patients and the incidence of postoperative complications when carbon dioxide pneumoperitoneum is established.Therefore the heating treatment must be closely monitored before the operation in order to maintain body temperature and to reduce the incidence of postoperative shivering after the operation.When carbon dioxide retention occurs,timely blood gas analysis and anesthetic intervention are crucial to the safety of the patients.

Objective:To compare the efficacy of different operations in treating pa-tients with previous cesarean scar defect(PCSD). Methods:Sixty-nine cases of PCSD were se-lected as study objects from Shenzhen Maternity and Child Healthcare Hospital of Southern Medical University,and divided into three groups due to different operation:18 cases were trea-ted with hysteroscope (group A),23 cases were treated with hysteroscopy combined with lapa-roscopy(group B),28 cases were treated with transvaginal uterine scar diverticulum resection(group C). Results:Menstruation recovery after surgery:the response rate of group B(87% ) and group C (86% ) were significantly higher than that of group A(50% )(P<0. 05). Postop-erative PCSD improvement:The response rate of group B(91% ) and group C (86% ) were sig-nificantly higher than that of group A(33% ). There was no significant difference between group B and group C (P>0. 05). Conclusion:In therapeutic efficacy of PCSD,hysteroscopy combined with laparoscopy are similar with transvaginal uterine scar diverticulum resection and better than hysteroscope. Although three kinds of operation have different advantages and disadvantages,the operation method should be chosen depending on the individual condition to reach the best out-come.

Objective To investigate the expression of human papillomavirus E2 mRNA in cervical cytologi-cal specimens as well as the role of that in cervical carcinogenesis and its clinical significance in screening and evalat-ing prognosis of cervical lesions.Methods The expression of E2 mRNA in 582 cases of cervical cytological speci-mens with high risk human papillomavirus infection and cytological diagnosis of NILM,ASCUS and LSIL,was detected by RT -PCR.Thereafter,all cases were divided into the four groups by colposcopy and histopathological confirmation, including 414 cases of cervicitis,95 cases of CINⅠ,51 cases of CINⅡ,20 cases of CINⅢ and 2 cases of invasive cervical carcinoma as well.Results The expression of HPV -E2 mRNA decreased dramatically corresponding with pathological upgrading from groups of cervicitis to invasive cervical carcinoma (χ2 =132.72,P <0.05).The sensitiv-ity,specificity,positive predictive value and negative predictive value of HPV -E2 mRNA for screening potential cervical lesions in group of NILM,ASCUS and LSIL were 77.2%,96.8%,75.6%,96.0% and 81.0%,91.7%, 79.1%,92.6% as well as 95.9%,93.4%,94.7%,95.0% respectively.Conclusion Deletion of HPV -E2 induced by genetic disruption played an important role in the early stage of malignant transformation of cervical epithe-lial cells.Therefore,detection of the levels of HPV -E2 mRNA expression might be clinically valuable for the screen-ing and evaluating of prognosis in cervical lesions.

Objetctive:To explore the effects,complications and recurrence rates of different therapies of endometrial polyps with hysteroscopy.Methods:Retrospective analysis of 162 cases with different surgical hysteroscopy for endometrial polyps in patients with clinical data. Results:3 groups of patients were successfully operated,with no complications.Variation in the postoperative period,the simple hysteroscopic resection of endometrial polyps(TCRP) and TCRP plus drugs in combination therapy to reduce menstrual,and hysteroscopy dilatation and curettage after location,the difference was statistically significant.C group patients with low recurrence rate(6%) .Infertility hysteroscopic surgery in 28 patients,13 cases after a successful pregnancy.Conclusion:3 methods for endometrial polyps are safe and effective,which simply TCRP and TCRP plus drug combination therapy is better,but after dilatation and curettage hysteroscopy locate a high recurrence rate.Hysteroscopic surgery to remove endometrial polyps can significantly improve the pregnancy rate of endometrial polyps lead to infertility.

Objective:To analyze the clinical manifestations and outcomes of mothers and infants in cases with hypertensive disorder complicating pregnancy(HDCP) combined with HELLP syndrome,explore effective diagnostic and treatment methods.Methods:The clinical data of 32 cases with HELLP syndrome were analyzed retrospectively,including 18 cases of complete HELLP syndrome and 14 cases of partial HELLP syndrome;10 cases of type 1 HELLP syndrome and 22 cases of type 2 HELLP syndrome;the laboratory indexes,clinical manifestations,complications of mothers and infants and perinatal outcomes were compared.Results:The platelet counts,hepatic enzymes and bilirubin levels in complete HELLP syndrome group and type 1 HELLP syndrome group were significantly higher than those in partial HELLP syndrome group and type 2 HELLP syndrome group,especially lactate dehydrogenase level(P<0.05).The complications of parturient women included disseminated intravascular coagulation(DIC),acute left heart failure,acute renal failure,placental abruption,severe anemia,postpartum hemorrhage,ascites,pericardial effusion and pleural effusion;there was significant difference in complications of parturient women between complete HELLP syndrome group and partial HELLP syndrome group(P<0.05),and there was significant difference between type 1 HELLP syndrome group and type 2 HELLP syndrome group;the perinatal mortality in complete HELLP syndrome group was 38.9%,and the perinatal mortality in partial HELLP syndrome group was 14.3%,there was significant difference(P<0.05);the perinatal mortalities in type 1 HELLP syndrome group and type 2 HELLP syndrome group were 50% and 18.2%,respectively,there was significant difference.Conclusion:The disease conditions of complete HELLP syndrome group and type 1 HELLP syndrome group are more severe then those of partial HELLP syndrome group and type 2 HELLP syndrome group,early diagnosis,comprehensive treatment and termination of pregnancy timely may improve the prognosis of patients with HELLP syndrome.

目的:探讨欣母沛预防和治疗宫缩乏力所致产后出血的效果。方法:选择顺产的孕妇300例,随机分为3组:欣母沛+缩宫素组、缩宫素组、对照组,每组100例。应用称重法计算产时及产后2h、24h、36h、48h的出血量,分析各组产后平均出血量及分娩前后血红蛋白的变化。结果:缩宫素组和对照组产后平均出血量、产后出血发生率均明显高于欣母沛+缩宫素组(P<0.05),产后24h血红蛋白平均值亦明显低于欣母沛+缩宫素组(P<0.05)。结论:欣母沛具有减少产后出血和促进子宫收缩的作用。

Objective To study the effects of hysteroscope combined with abdominoscope on the diagnosis and therapy of female infertility.Methods Clinical data of being diagnosed and therapied with hysteroscope and abdominoscope in 374 infertility cases were retrospectively analyzed.Explained the etiological factors and compared the positive rates among hysteroscope combined with abdominoscope,hysteroscope alone and abdominoscope alone.The patency rates were compared between hysterosalpingography and hydrotubation,and the recanalization rates of uterine tubes after operations were observed.Results The main causes of female infertility were pelvic affixion(50.80%),salpingemphraxis(42.25%),pelvic endometriosis(25.67%),endometrial polypus (13.90%),polycystic ovary(11.76%)and hysteromyoma(6.42%).The positive rate of hysteroscope combined with abdominoscope was obviously higher than the others.There was a significant deviation of oviductal patency rates between hysterosalpingography and hydrotubation.The recanalization rate of uterine tubes after the operation was 40.92%.Conclusion Hysteroscope combined with abdominoscope could comprehend the etiopathogenisis and heal the diseases of infertility in cavitas pelvis and uterine cavity with advantage of directly,exactly and overall.