An 8-week feeding trial was conducted to investigate the effect of selenium-yeast (Se-yeast) supplementation on replacing fishmeal with soy protein concentrate (SPC) in diets for golden pompano (Trachinotus ovatus). The control diet (C) contained 400 g/kg fishmeal, of which 40% and 80% of the fishmeal in diet C were substituted by SPC, with or without 1 g/kg Se-yeast supplementation (four diets, R40, R80, R40 + Se, and R80 + Se, were formulated). The weight gain of the fish-fed diet C showed no obvious difference from that of the fed-diet R40 + Se, although it was higher than that of the fish-fed diets R40, R80, and R80 + Se. Fish-fed diets R40 + Se and R80 + Se exhibited higher weight gain and nitrogen and phosphorus retention efficiencies, whereas they had relatively lower feed intake, feed conversion ratio, and nitrogen and phosphorus waste outputs than fish-fed diets R40 and R80. No statistical differences were found in condition factor and whole-body components either between fish-fed diets R40 and R40 + Se or between fish-fed diets R80 and R80 + Se. Our research suggests that it is feasible to reduce dietary fishmeal for golden pompano to 240 g/kg with SPC and 1 g/kg Se-yeast inclusion.

采集钱塘江(QT)、武义(WY)、高塘湖(GT)、肇庆(ZQ)和南宁(NN)5个不同群体的日本沼虾(Macrobrachium nipponense)样本,并对每个群体的线粒体COI基因进行测序和分析,以此了解各群体间的遗传差异.结果表明,日本沼虾COI基因序列中,保守位点420个,变异位点222个,简约信息位点123个,碱基缺失或插入位点27个,平均转换与颠换比值2.78.COI基因序列中(A+T)的含量明显高于(G+C)的含量,碱基组成具有明显的AT偏倚性.在67个个体中定义了65种单倍型,来自NN群体和QT群体的单倍型形成了2个主要分支.基于遗传距离构建的系统发育树和聚类树表明,QT群体和GT群体遗传距离较近,与ZQ群体和NN群体相比遗传距离较远,WY群体与QT、GT群体的遗传距离较小.研究结果可为日本沼虾种质资源的合理开发利用提供分子生物学依据.

近些年,随着我国水产养殖业的快速发展,高密度养殖及渔用配合饲料的大量使用,导致池塘养殖水体的富营养化和养殖鱼脂肪大量积累(Tanamati A等, 2009).水体富营养化对养殖鱼的品质、风味和健康等也产生负面影响.脂肪过度积累对鱼类肌肉营养组成、内脏脂肪含量、体色、风味及健康等也产生负面影响,进而影响市场接受度和渔业经济效益(Johansson L 等, 2000).

An 8-week feeding trial was conducted to investigate the effects of different dietary selenium yeast levels on growth, nutrient retention, waste output, and antioxidant capacity of juvenile triangular bream (Megalobrama terminalis). Five isonitrogenous (320 g/kg crude protein) and isolipidic (65 g/kg crude lipid) diets were formulated, with supplementation of graded levels of selenium yeast at 0 (diet Se0), 1 (diet Se1), 3 (diet Se3), 9 (diet Se9), and 12 g/kg (diet Se12). No significant differences were found in initial body weight, condition factor, visceral somatic index, hepatosomatic index, and whole body contents of crude protein, ash, and phosphorus among fish fed different test diet. The highest final body weight and weight gain rate were found in fish fed diet Se3. The specific growth rate (SGR) is closely related to dietary selenium (Se) concentrations with a relationship described as SGR = –0.0043 Se2 + 0.1062 Se + 2.661. Higher feed conversion ratio was found, while lower retention efficiencies of nitrogen and phosphorus were found in fish fed diets Se1, Se3, and Se9 than in fish fed diet Se12. Contents of selenium in whole body, vertebra, and dorsal muscle increased with dietary supplementation of selenium yeast increased from 1 mg/kg to 9 mg/kg. Lower nitrogen and phosphorous waste was found in fish fed diets Se0, Se1, Se3, and Se9 than in fish fed diet Se12. Fish fed diet Se3 exhibited the highest activities of superoxide dismutase, glutathione peroxidase, and lysozyme while the lowest malonaldehyde content in both the liver and kidney. Our results showed that the optimal dietary selenium requirement for triangular bream should be 12.34 mg/kg based on the nonlinear regression on SGR, and fish fed diet Se3 in which selenium concentration (8.24 mg/kg) was close to the optimal requirement displayed the best growth performance, feed nutrient utilization, and antioxidant capacity.

The present study was conducted to examine the effects of taurine supplementation on feed intake, growth, feed utilization, body composition, waste output, hepatic antioxidant enzymes, and intestinal microflora of Largemouth Bass Micropterus salmoides that were fed a low fish meal diet. Three isoproteic (49% crude protein) and isolipidic (9.5% crude lipid) diets were formulated: one diet contained 40% fish meal and served as the control (FM diet), and 60% of the fish meal in the FM diet was replaced by soybean meal either without taurine supplementation (SBM diet) or with 0.5% taurine supplementation (SBM+T diet). Lower feed intake, final body weight, weight gain, condition factor, and hepatosomatic index and a higher feed conversion ratio (FCR) were found in fish that received the SBM diet compared to fish that were fed the FM diet, but no significant difference was found in feed intake, final body weight, weight gain, FCR, condition factor, or hepatosomatic index between fish in the FM and SBM+T diet groups. No significant differences were found in nitrogen retention efficiency, body composition, nitrogen waste, or activities of total antioxidant capacity, superoxide dismutase, and glutathione peroxidase among fish fed the different test diets, and the alpha diversity of intestinal microbiota also did not differ among diet groups. The abundance of Proteobacteria and Firmicutes increased while the abundance of Fusobacteria decreased in the SBM diet group relative to the FM diet group; in contrast, dietary taurine (SBM+T diet) restored the abundance of Proteobacteria to a level similar to that in fish receiving the FM diet. This study suggests that the dietary fish meal level for Largemouth Bass can be reduced from 40% to 16% if soybean meal is used as an alternative protein with 0.5% taurine supplementation.

主要组织相容复合体(major histocompatibility complex,MHC)是一类具有高度多态性的分子,在脊椎动物的先天免疫应答中起到重要作用.根据已报道鱼类的MHCⅠα基因序列设计特异性引物,通过RT-PCR和RACE技术克隆三角鲂MHCⅠα基因cDNA序列全长,命名为Mete-UAA,并对其进行生物信息学分析.结果显示,Mete-UAA全长为2102 bp,包含1044 bp的开放阅读框,编码347个氨基酸.编码的MHCⅠα蛋白质预测分子量为38699.18,等电点5.23,含有16个氨基酸组成的信号肽,具有亲水性,定位于细胞膜上,具有1个N-糖基化、3个O-糖基化和39个磷酸化的潜在位点.氨基酸序列同源性比对发现,Mete-UAA氨基酸序列与团头鲂同源性最高为75.79％,与草鱼同源性稍低于团头鲂,为75.25％.Mete-UAA具有MHCⅠα的典型结构特征,包含1个前导肽、3个胞外结构域、1个跨膜区和1个胞质区.二级结构分析显示,Mete-UAA蛋白质中α-螺旋、β-转角、延伸链和无规则卷曲所占的比例分别为25.94％、9.22％、26.80％和38.04％.基于转录组学分析表明,感染嗜水气单胞菌(Aeromonas hydrophila)后三角鲂MHCⅠα基因表达呈总体下降趋势.本研究从三角鲂肝中成功克隆MHCⅠα基因,分析了其生物信息学特征,为后续研究MHCⅠα参与调控三角鲂先天免疫应答的机制提供理论基础.

试验旨在研究黑水虻幼虫干虫、虫粉替代鱼粉对乌鳢生长性能、饲料利用及肌肉营养组成的影响.选取初体重为(6.87±0.62)g的乌鳢幼鱼135尾,随机分成3组,每组3个重复,每个重复15尾鱼.分别用黑水虻幼虫干虫(BSF,粗蛋白33.30％、粗脂肪39.20％)、黑水虻幼虫虫粉(DBSF,粗蛋白53.00％、粗脂肪11.80％)替代基础饲料(鱼粉含量40％)50％的鱼粉.试验包括鱼粉组(D0组)、干虫组(D1组)和虫粉组(D2组)三种硬颗粒缓沉性饲料,开展为期60 d的养殖试验.结果表明:黑水虻干虫和虫粉替代50％鱼粉对乌鳢生长性能、饵料系数和成活率等无显著性影响(P>0.05).与鱼粉组(D0组)相比,黑水虻干虫对乌鳢的摄食率有显著的负面影响(P<0.05),脏体比显著提高(P<0.05).黑水虻干虫和虫粉替代试验饲料50％鱼粉对鱼体和肌肉的粗蛋白含量无显著性影响(P>0.05),干虫组(D1组)鱼体粗脂肪含量显著高于其他组(P<0.05),肌肉粗脂肪显著高于鱼粉组(P<0.05).替代50％鱼粉后干虫组(D1组)和虫粉组(D2组)肌肉部分必需氨基酸含量、必需氨基酸总量和非必需氨基酸及呈味氨基酸含量都有所降低.替代50％鱼粉后,肌肉脂肪酸中月桂酸(C12??0)含量和单不饱和脂肪酸总量显著提升(P<0.05),EPA、DHA及多不饱和脂肪酸含量显著降低(P<0.05),D2组多不饱和脂肪酸含量高于D1组.研究结果揭示,黑水虻干虫、虫粉替代乌鳢饲料50％鱼粉对其生长性能和饲料利用无显著性影响,但对其肌肉氨基酸和脂肪酸组成产生负面影响,同时说明黑水虻虫粉替代鱼粉补充鱼油后乌鳢肌肉多不饱和脂肪酸含量提升.

An 8-week feeding trial was conducted to investigate the effects of dietary zinc levels on growth, nutrient retention, waste output and nonspecific immunity of juvenile snakehead Channa argus. Six isonitrogenous (485 g/kg crude protein) and isolipidic (106 g/kg crude lipid) diets were formulated, with supplementation of graded levels of hydrated zinc sulphate at 0 (diet Zn0), 0.2 (diet Zn0.2), 0.4 (diet Zn0.4), 0.7 (diet Zn0.7), 1.0 (diet Zn1.0) and 1.3 g/kg (diet Zn1.3), which provided the actual dietary zinc concentration of 56, 99, 140, 235, 301 and 375 mg/kg respectively. The highest weight gain rate was found in fish fed diet Zn0.7. Lower feed intake and feed conversion ratio, while higher retention efficiencies of nitrogen and phosphorus, were found in fish fed diets Zn0.2, Zn0.4 and Zn0.7 than in fish fed diets Zn0 and Zn1.3. Zinc retention efficiency decreased with the increase IN dietary zinc levels. Contents of zinc in whole body and spinal bone increased with dietary hydrated zinc sulphate increased from 0 to 0.7 g/kg. Higher nitrogen and phosphorous waste were found in fish fed diets Zn0, Zn1.0 and Zn1.3 than in fish fed diets Zn0.2, Zn0.4 and Zn0.7, and lower zinc waste was found in fish fed diets Zn0, Zn0.2, Zn0.4 and Zn0.7 than in fish fed diets Zn1.0 and Zn1.3. Higher serum C4 and IgM were found in fish fed diets Zn0.7, Zn1.0 and Zn1.3 than in fish fed diet Zn0, Zn0.2 and Zn0.4. Our results showed that dietary supplementation of hydrated zinc sulphate needed to promote fast growth, keep good nutrient retention and immunological capacity, and reduce waste discharge in juvenile snakehead should be 0.7 g/kg (235 mg/kg zinc concentration).

为探究丝兰粉对初始体重为(16.0±0.8)g的三角鲂幼鱼生长、非特异性免疫及抗氧化指标的影响,在三角鲂基础饲料中分别添加0(S0)、0.05％(S1)、0.15％(S2)、0.25％(S3)、0.35％(S4)和0.45％(S5)的丝兰粉,连续投喂56 d后取样,测定相关的生理生化指标.研究结果表明,随着丝兰粉添加水平的增加,三角鲂的终末体重和体增重呈先上升后下降的趋势.与对照组S0相比,实验组S2的摄食率显著升高,而实验组S2和S3饲料系数显著降低(P<0.05).与对照组S0相比,实验组S1、S2、S3、S4、S5肝脏的溶菌酶、碱性磷酸酶、谷丙转氨酶活性均无显著差异(P>0.05);而实验组S2和S3肝脏的谷草转氨酶活性则显著降低(P<0.05).血清方面,与对照组S0相比,实验组S2和S3血清的溶菌酶和碱性磷酸酶活性显著升高,而其酸性磷酸酶、谷丙转氨酶、谷草转氨酶活性则显著降低(P<0.05);实验组S1、S2、S3、S4、S5血清的超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)显著高于对照组S0(P<0.05);随着丝兰粉添加水平的增加,实验鱼血清中的丙二醛(MDA)呈现先下降后上升的趋势(P<0.05).研究结果揭示,添加0.15％～0.25％丝兰粉可以促进三角鲂的生长表现,并提高其肝脏和血清的非特异性免疫及抗氧化指标.

近年来,我国水产养殖集约化程度不断提升,池塘循环水跑道养殖、集装箱养殖、工厂化养殖等一批新型养殖模式发展迅猛.在长期高密度拥挤养殖环境中,鱼长期处于应激状态,容易产生密度胁迫应激,引起生理功能紊乱、免疫能力下降等问题,对渔业经济发展和养殖生态环境产生负面影响.因此本试验针对大规格三角鲂鱼种,研究不同养殖密度对三角鲂应激水平、消化酶活力及抗氧化能力的影响,从而为进一步研究和养殖三角鲂提供帮助.

An eight-week feeding trial was conducted to examine the optimal dietary protein and lipid levels for juvenile hybrid sunfish (Lepomis cyanellus female x Lepomis macrochirus male). A 5 x 2 factorial layout containing five protein levels (300, 340, 380, 420 and 460 g kg(-1)) and two lipid levels (30 and 80 g kg(-1)) was designed. Fish (initial body weight 1.21 +/- 0.06 g) were fed the test diets twice daily. The final body weight (FBW) and per cent weight gain (PWG) in fish increased with dietary protein level from 300 to 420 g kg(-1), and afterwards decreased with dietary protein level from 420 to 460 g kg(-1). Feed intake and feed conversion ratio (FCR) significantly declined with increase dietary protein level. Fish fed at 380 and 420 g kg(-1) dietary protein level exhibited higher nitrogen retention efficiency (NRE) and phosphorus retention efficiency (PRE), however, lower nitrogen and phosphorus wastes, than in fish fed at 300 g kg(-1) dietary protein level. For different dietary lipid levels, the higher FBW, PWG, NRE and PRE, but the lower feed intake, FCR, nitrogen and phosphorus wastes, were found in fish fed at 80 g kg(-1) dietary lipid level than those in fish fed at 30 g kg(-1) dietary lipid level. At the end of feeding trail, the contents of body moisture and lipid were significantly influenced by dietary lipid level. According to these results, a diet containing 420 g kg(-1) protein and 80 g kg(-1) lipid is recommended for efficient growth of juvenile hybrid sunfish.

硒是生物体正常生长繁育所必需的微量元素,具有十分重要的生理作用.饲料中硒缺乏或过量均会对养殖动物造成不良影响.文章简单概述硒的生物功能、存在形式及其对水产养殖动物生长、饲料利用、鱼体组成、消化功能、抗氧化能力和免疫力等方面影响,旨在为硒在水产饲料工业中的应用提供参考.

This study determined the mitochondrial genome (mitogenome) of Chanodichthys mongolicus from China's Qiantang River and analyzed its phylogenetic history in the Subfamily Cultrinae. Next-generation sequencing was used to obtain the mitogenome of C. mongolicus, GenBank Accession Number MZ032228. The mitochondrial genome length of C. mongolicus from China's Qiantang River is 16,622 bp. The genome contains 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two central noncoding regions (the control region and the origin of light strand replication). Based on BLAST comparisons, the sequence identity of C. mongolicus MZ032228 from China's Qiantang River was 99.84% to that of Ancherythroculter wangi MG783573 from China's Nei River, 99.75% to C. mongolicus AP009060 from Russia's Black River. The phylogenetic analysis is consistent with BLAST comparisons in confirming that A. wangi MG783573 and C. mongolicus MZ032228 show a high genetic similarity. This study also confirms mitochondrial DNA heteroplasmy in C. mongolicus for the first time and documents 35 heterogeneous loci that were detected.

为利用基因分型测序(GBS)技术对三角鲂、翘嘴鲌、蒙古鲌及其杂交子代的遗传结构进行分析.本研究采用GBS技术对三角鲂、翘嘴鲌、蒙古鲌及其杂交子代(F1)共6个个体的鳍条提取总DNA进行双酶切简化基因组测序,使用Stacks软件构建SNP比对参考基因组进行分析.结果显示,6个个体共产生clean data 7.01 GB,平均每个样品1.17 GB;将所有样本作为一个群体检测SNP变异,共检测出SNP位点399145个,质控过滤后得到SNP位点97911个.基于亲本及其子代的SNP位点分析表明,亲本及其子代的平均观测杂合度(Ho)为0.3394,平均期望杂合度(He)为0.2853,平均多态性信息含量(PIC)为0.2737,平均核苷酸多样性(Pi)为0.3727,平均最小等位基因频率(MAF)为0.2472,平均可分型样品比例(genotype rate)为93.94％.遗传分化指数和遗传距离分析结果表明,三角鲂、翘嘴鲌、蒙古鲌及其杂交子代间的分化指数为0.3096～0.8940,遗传距离为0.3705～2.2443.使用最大似然(ML)法构建的系统进化树显示,三角鲂、翘嘴鲌、蒙古鲌及其杂交子代区分明显.本研究通过对三角鲂、翘嘴鲌、蒙古鲌及其杂交子代的遗传结构分析,将为鲂鲌鱼类的杂交选育提供更多的遗传学数据.

本试验以鲫鱼源维氏气单胞菌为宿主菌,采用双层平板法从发病鲫鱼肠道中分离维氏气单胞菌的烈性噬菌体,并通过透射电子显微镜对噬菌体的形态进行了观察;同时还对噬菌体的生物学特性进行了测定,包括最适感染复数(MOI)、一步生长曲线、热稳定性、pH稳定性等.结果 显示,本试验成功分离获得了1株鲫鱼源维氏气单胞菌噬菌体SJTJYCA01,该噬菌体的噬菌斑圆形透明,边缘清晰整齐,无晕环,其头部直径在45 ～60 nm,尾部长16～26 nm,属于短尾噬菌体科;噬菌体的最适MOI为0.1;感染宿主菌的潜伏期为20 min,裂解期为40 min,裂解量为57;在温度40℃以下和pH 5～10较为稳定.综上所述,SJTJYCA01为1株鲫鱼源维氏气单胞菌烈性噬菌体,该噬菌体能很好的适应水产养殖环境,在水生动物细菌性疾病防控中具有极大的应用价值.本试验丰富了水产致病菌噬菌体的菌种资源,并为进一步探索噬菌体防控技术提供了科学依据.

This study developed and characterized 68 novel polymorphic microsatellite markers from black Amur bream Megalobrama terminalis by next-generation sequencing. Variability was tested on 36 individuals collected from Qiantang River, Zhejiang Province, China. The number of alleles per locus ranged from 2 to 13. Observed heterozygosity ranged from 0.028 to 0.944, whereas the expected heterozygosity ranged from 0.028 to 0.887. Polymorphism Information Content ranged from 0.027 to 0.862. Moreover, 53 microsatellites were in agreement with Hardy–Weinberg equilibrium. Twenty-eight pairwise tests in 33 microsatellite loci indicated linkage disequilibrium. These microsatellites are a valuable tool for further genetics studies of this species.

试验旨在研究黑水虻替代饵料鱼粉对乌鳢生长性能、抗氧化及免疫指标的影响.选取初体重为(6.87±0.62)g的鸟鳢幼鱼135尾,随机分为3组,每组3个重复,每个重复15尾鱼.对照组乌鳢饲喂基础饵料,试验组分别使用黑水虻干虫(D1组)和黑水虻虫粉(D2组)替代基础饵料中50％的鱼粉.试验期60 d.结果 表明,与对照组相比,D1、D2组乌鳢血清超氧化物歧化酶活性显著升高(P＜0.05);血清丙二醛含量显著降低(P＜0.05).肾脏酸性磷酸酶活性、补体C3和C4含量均显著高于对照组(P＜0.05).D1组乌鳢的脏体比显著高于其他组(P＜0.05).研究表明,黑水虻虫粉可以增强乌鳢抗氧化能力和免疫功能,且黑水虻干虫对鸟鳢幼鱼抗氧化和免疫能力的促进作用更明显.

Background The mitogenomic heteroplasmy is the presence of multiple haplotypes in the mitochondria, which could cause genetic diseases and is also associated with many critical biological functions. The topmouth culter ( Culter alburnus Basilewsky, 1855) is one of the most important freshwater fish in the family of Cyprinidae in China. At present, there are no reports on the topmouth culter's mtDNA heteroplasmy and the existence of which is not known. Methods and results This study aimed to analyze the mitogenomic heteroplasmy in the topmouth culter by the next-generation sequencing of the fins' total DNA. The results confirmed the existence of the heteroplasmy and indicated the presence of the extensive heteroplasmy in the topmouth culter's mitogenome. There were 38 heteroplasmic variations in the protein-coding genes from the three specimens, with 33 non-synonymous substitutions accounting for 86.84% and five synonymous substitutions accounting for 13.16%. Among them, the ND 6 had the most heteroplasmic variations but only one synonymous substitution. After removing the putative nuclear mitochondrial DNA fragments, the ratio of primary haplotype in the three specimens was 43.89%, 74.72%, and 32.76%, respectively. The three specimens contained 21, 7, and 21 haplotypes of the mitogenomes, respectively. Due to the extensive heteroplasmy, we reconstructed the phylogenetic tree of the topmouth culter using the RY-coding method, which improved the performance of the phylogenetic tree to some extent. Conclusions This study reported the mitogenomic heteroplasmy in the topmouth culter and enhanced the knowledge regarding the mitogenomic heteroplasmy in phylogenetic studies. As the topmouth culter is a commercial species, the mitogenomic heteroplasmy is crucial for the fisheries management of the topmouth culter.

The effects of replacing dietary fishmeal by soybean meal with taurine supplementation on feed intake, growth, feed utilization, body composition and waste output of largemouth bass were examined. A control diet (C) contained 400 g/kg fishmeal, 20%, 40% and 60% of fishmeal in diet C was replaced by soybean meal, with or without 5 g/kg taurine supplementation (abbreviated as S20 + T, S40 + T, S60 + T, S20, S40 and S60, respectively). Significantly lower weight gain was found in fish fed diet S60 compared with fish fed diet C (p < 0.05), while no significant difference was found in weight gain between fish fed diet C and fish fed diets S20, S20 + T, S40 + T and S60 + T (p > 0.05). No statistically differences were found in feed intake and feed conversion ratio between fish fed diet C and fish fed diets S20, S20 + T, S40, S40 + T and S60 + T (p > 0.05). No significant differences were found in nitrogen retention efficiency, body crude protein content and nitrogen waste among fish fed different test diets (p > 0.05). This study suggests that dietary fishmeal level for largemouth bass can be reduced to 240 g/kg and further reduced to 160 g/kg with taurine addition, with soybean meal used as a fishmeal substitute.

为了研究钱塘江流域三角鲂（Megalobrama terminalis Richardson）线粒体基因组结构特征及鲌亚科鱼类的系统进化关系,通过PCR扩增、测序、软件拼接获得了钱塘江三角鲂线粒体基因组全序列,GenBank登录号为MN725725。结果表明,钱塘江三角鲂线粒体基因组序列全长为16 621 bp,碱基组成分别为A（31.23%）、G（16.17%）、C（27.87%）和T（24.73%）;共有13个蛋白编码基因,22个tRNA基因,2个rRNA基因,NAD6、tRNA-Gln、tRNA-Ala、tRNA-Asn、tRNA-Cys、tRNA-Tyr、tRNA-Ser （UCN） 、tRNA-Glu和tRNA-Pro等基因编码在L链上,其余基因均在H链上编码。钱塘江三角鲂线粒体基因组全序列与蛋白编码基因的A+T含量分别为55.97%和55.86%,具有明显的AT偏好性。线粒体基因中存在2个散在重复序列,分别位于线粒体控制区中终止结合序列区的前端和控制区3′的末端。在22个tRNA基因中,除了tRNA-Ser （AGY） 外,均具有典型的三叶草二级结构。基于BLAST比较,钱塘江三角鲂与黑龙江流域的三角鲂一致性为99.76%,与珠江流域三角鲂一致性为99.87%。基于15种隶属于7属的鲌亚科鱼类线粒体基因组全序列构建的系统进化树,鲂属与鳊属亲缘关系比与鲌属的亲缘关系上较近,与■属、半■属和细鳊属亲缘关系上较远。