BackgroundGuizhi Fuling (GZFL) pill, a traditional Chinese herbal formula including Semen Persicae, Ramulus Cinnamomi, Poria, Radix Paeoniae Alba, and Cortex Moutan, has been widely applied in the management of gynecological diseases.ObjectiveTo evaluate the add-on effect of the GZFL formula for treating reduced fertility potential in women with polycystic ovary syndrome (PCOS) by conducting a systematic review and meta-analysis.MethodsTwo reviewers independently searched the PubMed, Embase, Cochrane Library, Wanfang, SinoMed, and CKNI databases until 09/11/2022. Eligible studies were randomized controlled trials (RCTs) of the GZFL formula plus Western medicine versus the Western medicine for treating PCOS. The primary endpoint was the ovulation, pregnancy, and miscarriage rate. The secondary endpoints included the serum follicle-stimulating hormone (FSH), total testosterone, luteinizing hormone (LH), estradiol, and homeostasis model assessment insulin resistance (HOMA-IR).ResultsThere were 16 RCTs with 1,385 patients identified. The GZFL formula plus Western medicine significantly improved the ovulation rate (risk ratios [RR] 1.24; 95% confidence intervals [CI] 1.15–1.34) and pregnancy rate (RR 1.53; 95% CI 1.38 to 1.69) than the Western medicine alone. Adjuvant treatment with the GZFL formula also significantly decreased the serum FSH (mean difference [MD] -0.48 U/l; 95% CI -0.80 to -0.15), total testosterone (standard mean difference [SMD] -1.07; 95% CI -1.71 to -0.44), LH level (MD -2.19 U/l; 95% CI -3.04 to -1.34), and HOMA-IR (MD -0.47; 95% CI -0.60 to -0.34). However, there was no significant difference in the miscarriage rate (RR 0.89; 95% CI 0.36–2.20) and serum estradiol level (SMD 0.34; 95% CI -0.25 to 0.94) between two groups.ConclusionsThe GZFL formula as adjuvant therapy can improve the ovulation and pregnancy rates in women with PCOS. Its beneficial effects may correlate with reducing FSH, total testosterone, and LH and ameliorating insulin resistance. However, more well-designed RCTs with larger samples and multicenter trials are required to confirm the current findings due to uncertainty of the evidence.Systematic review registrationPROSPERO identifier, CRD42022354530.

OBJECTIVE:To evaluate the impact of postoperative acute kidney injury (AKI) on early and long-term mortality in patients with acute aortic dissection by conducting a meta-analysis. METHODS:An extensive literature search was performed in PubMed and Embase databases until February 15, 2020. Observational studies that reported the associations between postoperative AKI and early (in-hospital and within 30 days) or long-term mortality in patients with acute aortic dissection were included. RESULTS:Seven studies comprising 1525 acute aortic dissection patients were identified. A random effect meta-analysis showed that postoperative AKI was significantly associated with higher risk of long-term mortality (risk ratio [RR] 2.32; 95% confidence interval [CI] 1.50-3.59). Subgroup analysis revealed that the pooled RR of long-term mortality was 1.42 (95% CI 0.90-2.22) for stage 1 AKI, 1.72 (95% CI 0.95-3.12) for stage 2 AKI, and 4.46 (95% CI 2.72-7.32) for stage 3 AKI, respectively. Furthermore, postoperative stage 3 AKI was associated with an increased risk of early mortality (RR 11.3; 95% CI 4.2-30.5). CONCLUSIONS:This meta-analysis provided clinical evidence that postoperative stage 3 AKI is associated with higher risk of early and long-term mortality, even after adjusting important confounding factors. However, the current findings should be interpreted with caution due to the retrospective nature and limited number of studies analyzed.

Studies have yielded contradictory results concerning the association between dehydroepiandrosterone sulfate (DHEAS) and mortality in the elderly population. This meta-analysis aimed to evaluate the association of low serum DHEAS level with cardiovascular or all-cause mortality in the elderly population. A comprehensive literature search was conducted in PubMed and Embase databases up to 4 February, 2019. Longitudinal observational studies reporting multivariate adjusted risk ratio (RR) and corresponding 95% confidence intervals (CI) for cardiovascular or all-cause mortality with respect to baseline low DHEAS level were included. Both fixed-effect and random effect model were used to pool the overall risk estimate. Methodological quality of the included studies was evaluated using a 9-point Newcastle-Ottawa Scale. Six prospective studies enrolling 6,744 individuals were identified. Five studies were graded as high methodological quality. When compared the lowest to the reference higher circulating DHEAS level, the pooled RR of all-cause and cardiovascular mortality was 1.46 (95% CI: 1.25-1.70) and 1.49 (95% CI: 1.11-1.99), respectively. Subgroup analysis indicated that the association of low DHEAS level with all-cause mortality risk was only found in men (RR 1.41;95% CI: 1.18-1.69) but not in women (RR 1.72; 95% CI: 0.99-2.99). This meta-analysis provides evidence that low circulating DHEAS level is associated with increased risk all-cause mortality in the elderly population.

Motherwort (YiMuCao), a traditional Chinese herb, has been shown beneficial effects for women's diseases. This meta-analysis aimed to evaluate the efficacy and safety of motherwort injection add-on therapy to carboprost tromethamine for prevention of post-partum blood loss. A systematic literature search was conducted in PubMed, Embase, Cochrane Library, CNKI, VIP and Wanfang from their inception to December 2017. Randomized controlled trials that determined the add-on effects of motherwort injection to carboprost for prevention of post-partum blood loss were eligible. Pooled risk ratio (RR) and mean difference (MD) with 95% confidence interval (CI) were used to summarize the effect sizes. Eight trials including 1276 pregnant women fulfilled the inclusion criteria. Prophylactic use of motherwort injection add-on therapy significantly reduced the post-partum 2 h (MD -127.5 mL; 95% CI -149.13 to -105.88) and 24 h (MD -146.85 mL; 95% CI -179.77 to -113.94) blood loss and incidence of post-partum hemorrhage (RR 0.28; 95% CI 0.17-0.45) than carboprost. Moreover, adjunctive treatment with motherwort injection significantly decreased the length of the third stage of labor (MD -3.41 min; 95% CI -4.33 to -2.49) and duration of lochia (MD -7.13 days; 95% CI -8.49 to -5.76). There was no statistical significant difference in the incidence of adverse events (RR 0.76; 95% CI 0.50-1.16). Prophylactic use of motherwort injection add-on therapy to carboprost tromethamine could reduce post-partum blood loss. However, more well-designed trials are necessary to confirm the findings of this study due to the methodological flaws of the included trials.

目的 通过临床观察比较绿茶和清水漱口对糖尿病患者种植体周围炎的预防效果,初步探讨绿茶作为糖尿病患者口腔护理液的可行性.方法 选择80例糖尿病患者,共计115颗种植体随机分为2组,分别用绿茶和清水漱口,3月后检测两组患者种植体改良出血指数(mSBI)、菌斑指数(PLI)探诊深度(PD)及Jemt牙间乳头指数,进行统计学分析.结果 绿茶漱口组患者的各项临床检查结果,除牙间乳头指数外,均显著优于清水漱口组(P<0.05).结论 绿茶漱口对糖尿病患者种植修复后种植体周围炎的发生具有一定预防作用.

Inconsistent results existed between the circulating asymmetric dimethylarginine (ADMA) concentration and preeclampsia. This meta-analysis aimed to compare the circulating ADMA concentration in patients with preeclampsia and healthy pregnant women. A systematic literature search was performed in PubMed, Embase, CNKI, VIP, and Wanfang database up to May 30, 2016. Case-control studies reporting the circulating ADMA concentration in preeclampsia patients and healthy pregnant women were selected. The pooled standard mean difference (SMD) with 95% confidence interval (CI) were used to estimate the relationship between circulating ADMA concentration and preeclampsia. Eighteen studies involving 879 preeclampsia patients and 994 healthy pregnant women were identified and analyzed. The pooled estimates from 11 studies showed that preeclampsia patients had an increased circulating ADMA concentration as compared with healthy pregnant women (SMD=1.15; 95% CI=0.75-1.55). Subgroup analysis indicated that the association was more pronounced in severe preeclampsia patients (SMD=2.41; 95% CI=1.43-3.39) than in the mild preeclampsia patients (SMD=1.62; 95% CI=0.52-2.71). The meta-analysis suggests that higher circulating ADMA concentration was observed to be higher in women with preeclampsia than that in the healthy pregnant controls. Elevated ADMA concentration is positively correlated to the severity of disease.

Objective To study the role and molecular mechanism of has-miR-16 in the development of tongue squa mous cell carcinoma (TSCC).Methods The expression of has-miR-16 was detected in 36 cases of TSCC tissues and matched normal tissues by using Quantitative real time PCR.The expression of has-miR-16 in TSCC cells (CAL-27 and SCC-9) and the normal human oral mucosa fibroblast cells (hOMF) was also analyzed by Quantitative real time PCR.The cell proliferation and migration of CAL-27 and SCC-9 were analyzed by MTT and wound healing assays after being transfected with has-miR-16 mimics or mimics control.The target gene of has-miR-16 was predicted by bioinformatics and verified by dual luciferase reporter assay.Results The expression of has-miR-16 in TSCC tissues was significantly lower than matched normal tissues (P ＜ 0.05).The expression of has-miR-16 was also down-regulated in CAL-27 and SCC-9 cells compared with hOMF cells (P ＜ 0.05).Over-expression of has-miR-16 inhibited cell proliferation and migration in CAL-27 and SCC-9 cells (P ＜ 0.05).MYB (Myeloblastosis oncogene) was the target gene of has-miR-16 in TSCC.Conclusion Has-miR-16 is down-regulated in TSCC tissue and cell lines;over-expression of has-miR-16 inhibites CAL-27 and SCC-9 cells proliferation and migration;has-miR-16 acts as tumor suppressor in TSCC,which may play its role via regulating MYB.

Objective To observe the clinical effect of antiplatelet drugs in elderly patients with coronary heart disease. Methods The clinical data of 110 elderly patients with coronary heart disease diagnosed and treated in our hospital between January 2013 and December 2014 were reviewed, and divided into 2 groups according to different treatments given. The 47 patients treated with Clopidogrel were assigned as control group, whereas the other 63 patients using the study group using dual antiplatelet drugs (Clopidogrel combined with Aspirin) for treatment as study group. The clinical efficacy and safety in the two groups were compared.Result sThe total effective rate in the study group was 90.48%, significantly higher than that in the control group, which was 72.34%, and the reduction of IRADP in the study group was more significant than that in the control group (P<0.05).Conclusion Dual antiplatelet drugs are with significant curative effect and higher safety in the treatment of elderly patients with coronary heart disease, thus it is worthwhile to be put into clinical practice.

Objective:The clinical effect comparison of Vitapex paste and ordinary calcium hydroxide paste used in appexification.Methods:60 cases were reviewed of our department from 2010 to 2014 outpatients,33 cases of male,27 cases of female,aged from 7~1 1 years old,a total of 60 teeth that the root hole had not yet fully developed due to illness or injury.31 were randomly divided into experimental group,and the others were control group.Two groups were operated appexification with Vitapex paste and ordinary calcium hydroxide paste separately after conventional complete root canal cleaning and root canal disinfection.Results:After 2 years follow-up,Vitapex paste group of 24 cases of success,and the success rate was 93.55%;Ordinary calcium hydroxide cataplasm group of 29 cases of success,and the success rate was 82.76%.There was significant difference (P < 0.05 ).Conclusion:As a root induction drug, Vitapex paste has better clinical effect than ordinary calcium hydroxide paste of young permanent teeth appexification.

目的 观察颌骨囊肿受累活髓牙保存术后的临床效果.方法 大型颌骨囊肿手术患者72例,随机分为研究组和对照组各36例,研究组X线片观察囊肿已累及但电活力测试正常患牙72颗,保留活髓不做根管治疗,对照组X线片观察囊肿己累及电活力测试正常患牙69颗行根管治疗.对比两组囊肿治疗疗效.结果 术后两组患者均无自觉症状,两年内随访均未见复发,研究组电活力测试为活髓牙的大多数牙牙髓保存,未出现异常,个别牙囊肿术后1周行根管治疗术.结论 颌骨囊肿的手术治疗过程中,如存在囊肿边缘受累活髓牙,对符合适应证的受累活髓牙可保留活髓,对颌骨囊肿的疗效无影响.

Objective To observe the effect of microwave combined with Recombinant Human Interferonα-2b Suppository in the treatment of chronic cervicitis.Methods The clinical data of 128 patients with chronic NCU were selected from those who had been receiving treatment in our hospital between August 2014 and August 2015 were reviewed, which, according to the treatment options, were divided into control group (n=58) and study group (n=70). The patients treated with conventional method were set as control group, whereas those given microwave combined with Recombinant Human Interferonα-2b Suppository as the study group. The curative effect, postoperative indicators and adverse reactions in the two groups were compared.Results The total effective rate in the study group was higher than that in the control group (P<0.05). The duration of vaginal discharge and bleeding in the two groups was both short, yet that in the study group was signiifcantly shorter than that in the control group (P<0.05).Conclusion Microwave combined with Recombinant Human Interferonα-2b Suppository is with deifnite curative effect in the treatment of chronic NCU.

目的:观察伢典凝胶去腐技术治疗乳牙龋病的临床效果.方法:筛选75例150颗中龋患牙作为研究对象.伢典组79颗患牙:应用伢典凝胶法去腐、充填.对照组71颗患牙:常规牙钻去腐、制备洞型、充填.结果:治疗过程中无疼痛率、患儿合作率,伢典组优于对照组(P＜0.05).伢典组充填6个月及1年后充填成功率分别为96.2％、84.5％,继发龋发生率分别为0.00％、9.86％.与对照组比较差异无统计学意义(P＞0.05).结论:伢典凝胶去腐技术治疗乳牙龋病减少了牙钻和麻醉的使用,减轻了患儿的疼痛和不适感,提高了治疗成功率.

Objective:To investigate the most important metastatic and prognostic factor in squamous cell carcinoma (SCC) of the tongue by means of analyzing the relationship between histologic grading of mode of invasion and other clinicopathological parameters. Methods:A total of 75 cases with SCCs of the tongue were studied by histological method with Hematoxylin-Eosin (H-E) stained. Relationship between differentiation (WHO grading) and mode of invasion (Y-K grading) with other clinicopathological parameters was analyzed. Results:Differentiation has correlation with gender and mode of invasion, but it has no correlation with age, clinical stage and lymph node metastasis. The mode of invasion was associated with lymph node metastasis (P<0.011) and differentiation (P<0.025), but it was not correlated with gender, age and clinical stage. Among 68 cases with SCC of the tongue in stage Ⅰ and Ⅱ, there were signiifcant difference (P<0.018) between mode of invasion and lymph node metastasis and but it was not associated with other parameters. Conclusion:This study indicates that the mode of invasion is a useful factor in predicting cervical lymph node metastasis and prognosis in SCC of the tongue. The application of diagnosis by the mode of invasion before surgery in the treatment of tongue SCC.

To study the influence of Er:YAG laser treatment on repairing effect of enamel and dentin treated by different surface managements.Eighty specimens were randomly divided into enamel and dentin groups,each group was assigned to 4 subgroups according to the different surface treatments:①Etching(37%phosphoric acid),②Er:YAG laser,③Self-etching adhesive,④Er:YAG laser+self-etching adhesive.Resin-modified glass-ionomer cement (RMGIC)was bonded to the previous treated surfaces.After storing in 37 ℃ incubator for 24 hours,shear bond strength was measured with a universal testing machine.In both enamel and dentin groups,3 and 4 subgroups showed no statistically significant differ-ence (P>0.05),showed higher value compared with the other subgroups(P<0.05).There was no statistically signif-icant difference between 1 and 2 subgroups(P>0.05 ).Er:YAG laser irradiation can replace the way that treated by 37% phosphoric acid etching.G-Bond adhesive may improve the bond strengths between tooth surface and RMGIC,and Er:YAG laser have no influence on that.