Magnesium (Mg) and its alloys were favored by biomedical practitioners thanks to availability of bioactivity and degradability. However, the mismatch between the degradation properties of Mg alloys and the rate of osteogenesis often led to implant failure and bacterial infections within the desired period. The goal of this study was to improve the corrosion resistance of Mg alloys, providing theoretical guidance for solving the problems of implantable Mg-based materials. In this experiment, we prepared a dense and uniform BTESPT/ TiO2 film layer on the surface of Mg substrate by electrochemically assisted deposition. The BTESPT/TiO2 film layer provided a physical barrier to avoid direct contact between AZ31 and the corrosive medium. When the addition amount was 2g/L TiO2, the coating had the best corrosion resistance behavior, its corrosion current density could be up to 9.973×10-8A/cm2. The BTESPT/TiO2 revealed good cell viability as well as osteogenic differentiation potential on MC3T3-E1 cells.

In a previous study, we found that oligodeoxynucleotide (ODN) YW002 could downregulate the synthesis of nitric oxide in RAW 264.7 cells, laying the experimental foundation for the subsequent application of ODN YW002 in periodontitis.

In the original publication, there was a mistake in Figure 3 as published [...]

镁基生物材料作为一种生物可降解材料,具有良好的机械强度、可降解性、骨传导性等特性,成为生物医用材料领域的研究热点.然而,这些材料在生理环境下的快速降解可能导致机械强度的过早丧失、皮下气肿和碱中毒等现象的发生,从而阻碍其在临床中的应用.针对以上缺点,研究者提出了许多表面处理策略,以抑制其降解速率,强化其生物活性,提高其生物相容性等相关性能.本文对近年来镁基材料表面功能化涂层的研究进展以及这些涂层在调节降解行为和生物功能方面的表现进行综述.

Magnesium (Mg) alloys, a degradable material, have beenstudied for medical applications due to their excellent mechanicaland chemical properties. However, their applications are limited byrapid corrosion. In this work, stearic acid and sodiumstearate were used to treat the silane-induced calcium phosphate dihydratecoating to improve its protection for the Mg alloy further withoutchanging the bone-like structure of calcium phosphate. The differenteffects of stearic acid treatment and sodium stearate treatment werecompared. Electrochemical test and immersion test results confirmedthat the corrosion resistance of the stearic acid-treated compositecoating was greatly enhanced with a reduced corrosion current densityby 3 orders of magnitude and hydrogen evolution reduced to 1/25 after14 days. The stearic acid-treated coating also exhibited improved in vitro biocompatibility corroborated by promoted cellviability and better cell morphology.

Objective:Bohai algae oil contains polyunsaturated fatty acids, such as EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid), which are the very important polyunsaturated fatty acids for the human body. In Traditional Chinese Cooking, all cooking methods cannot do without oils. However, the heat of cooking may always lead to formation of large amounts of decomposition products that affect the sensory, nutritional and functional quality of the oils to be unhealthy and the products being cooked, especially the food or edible oil rich in polyunsaturated fatty acids. Therefore, the objective of this study was to research the effect and change of heating on the quality characteristics of Bohai algae oil comparison with soybean oil and olive oil. Method:Quality characteristics such as the iodine values (IVs), peroxide values (POVs) and acid values (AVs) of Bohai algae oil, soybean oil and olive oil were measured, cooking conditions as factors of the study. Result:The POVs percentage changes of Bohai algae oil were larger than the IVs and AVs percentage changes. Bohai algae oil was better heated in a microwave oven at microwave P-20 within 3min, microwave P-60 and P-H1 within 2min, or in induction cooker at 1500w within 1min, or on electric stove (direct heated) within 2min. Conclusion:Bohai algae oil was suitable used for low-temperature and short-time cooking or for salad. This study has important significance for promoting the commercial value and extensive application of Bohai algae oil in daily cooking. It plays a theoretical significance role in Bohai algae oil's better processing and traditional chinese cooking. It can improve product quality to further expand the food processing research scope of Bohai algae oil and increase the richness, diversity and universality of edible methods of Bohai algae oil.

目前,临床上主要利用骨植入物来修复缺损的骨组织,骨植入物主要包括以钛合金、钴铬合金、镁合金为代表的金属材料以及生物医用陶瓷材料、生物医用高分子材料等.但由于目前我们所使用的植入物通常存在结合不良、排斥反应、感染等问题,因此植入物的表面改性逐渐成为了研究热点.植入物的表面改性主要分为物理改性、化学改性以及生物改性[1],其中,生长因子涂层作为生物改性的重要分支,已受到越来越多的关注.制备生长因子涂层目的是将生长因子保留在缺损区,使其作用时间延长,从而达到促细胞迁移的效果.骨植入物与周围骨组织的良好结合是决定治疗是否成功的基础,将骨生长因子运输到骨缺损处能够加快骨修复过程[2].本文通过对主要的骨生长因子涂层的研究进展进行总结,为生长因子涂层在骨组织再生的研究以及应用提供参考.

Objective: This study aimed to investigate the regulatory effect of N-isopropylacrylamide-modified polyethyleneimine (PEN)-delivered oligodeoxynucleotide (ODN) MT01 on bone regeneration in vitro and in vivo.Methods: A polyethylenimine (PEI) derivative, PEN, was constructed through Michael addition and employed as a carrier for ODN MT01 transfection. PEN/MT01 nanocomposites were characterized using agarose gel retardation assay, size distribution, zeta potential and transmission electron microscopy. The Cell Counting Kit-8 (CCK-8) assay was used to detect the effect of PEN on cell viability. Alkaline phosphatase (ALP) staining was used to detect the osteogenic differentiation ability of PEN/MT01 nanocomposite. Real-time quantitative PCR (q RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the regulatory effects of PEN/MT01 nanocomposite on osteogenic differentiation gene expression. Rat model was observed using the skull defect method and verified using micro-computed tomography (CT), serum biochemical indices, hematoxylin and eosin (H&E) staining and Immunohistochemistry (IHC).Results: PEN had good biological properties and could deliver MT01 well to achieve efficient transmission of MT01. PEN/MT01 nanocomposites were effectively transfected into MC3T3-E1 cells at a ratio of 6.0. CCK-8 assay displayed that PEN had no cytotoxicity to MC3T3-E1 cells. Additionally, PEN/MT01 nanocomposites could promote the expression of osteogenic genes. In vivo results revealed that PEN/MT01 nanocomposites could promote bone regeneration more effectively than the other groups.Conclusion: PEN has good biocompatibility and low toxicity, which is a good carrier for ODN MT01. PEN-delivered MT01 can be potentially employed as a useful approach to achieving bone regeneration.

Periodontitis is a long-term inflammatory illness and a leading contributor to tooth loss in humans. Due to the influence of the anatomic parameters of teeth, such as root bifurcation lesions and the depth of the periodontal pocket, basic periodontal treatment on its own often does not completely obliterate flora microorganisms. As a consequence, topical medication has become a significant supplement in the treatment of chronic periodontitis. Berberine (BBR) has various pharmacological effects, such as hypoglycemic, antitumor, antiarrhythmic, anti-inflammatory, etc. The target of our project is to develop a safe and non-toxic carrier that can effectively release berberine, which can significantly reduce periodontal tissue inflammation, and to investigate whether berberine thermosensitive hydrogel can exert anti-inflammatory and osteogenic effects by modulating phosphatifylinositol-3-kinase/Protein Kinase B (PI3K/AKT) signaling pathway. Consequently, firstly berberine temperature-sensitive hydrogel was prepared, and its characterizations showed that the mixed solution gelated within 3 min under 37 °C with a hole diameter of 10–130 µm, and the accumulation of berberine release amounted to 89.99% at 21 days. CCK-8 and live-dead cell staining results indicated that this hydrogel was not biotoxic, and it is also presumed that the optimum concentration of berberine is 5 µM, which was selected for subsequent experiments. Real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB)results demonstrated that inflammatory factors, as well as protein levels, were significantly reduced in the berberine-loaded hydrogel group, and LY294002 (PI3K inhibitor) could enhance this effect (p < 0.05). In the berberine-loaded hydrogel group, osteogenesis-related factor levels and protein profiles were visibly increased, along with an increase in alkaline phosphatase expression, which was inhibited by LY294002 (p < 0.05). Therefore, berberine thermosensitive hydrogel may be an effective treatment for periodontitis, and it may exert anti-inflammatory and osteogenic effects through the PI3K/AKT signaling pathway.

组织工程学和引导组织再生技术是牙周组织再生的常见手段,支架是组织工程的基本要素,引导组织再生依靠屏障膜实现.支架材料性能与其结构密切相关,复合材料能弥补单一材料存在的缺陷,性能更佳,能更好地促进牙周组织再生.综述了组织工程支架材料和引导组织再生屏障膜的应用研究进展.

脂联素是一种激素蛋白,在许多全身炎症性疾病中发挥抗炎作用.牙周炎是一种局部炎症性疾病,它会逐渐破坏牙周支持组织最终导致牙齿脱落.目前已经表明脂联素与牙周炎之间存在关联.本文综述了脂联素的基本特性及其在牙周炎中的作用,以及脂联素与伴全身疾病牙周炎的关系.

As a new implantable biomaterial, magnesium (Mg) alloy has a broad application prospect. However, it is urgent to solve the disadvantage of rapid degradation. In this work, a composite coating with flaky dicalcium phosphate dihydrate (DCPD) as the bottom layer and polycaprolactone (PCL) incorporated with titania (TiO2) as the sealing layer was synthesized by electrodeposition and spin coating. Due to the sealing effect of the PCL layer and the blocking effect of TiO2 nanoparticles on the corrosive solution, the corrosion resistance of the composite coating sample is significantly improved, and its impedance could reach 628 times that of Mg alloy, and the total hydrogen evolution amount of the composite coating is only 1/19 of that of Mg alloy after immersion for 14 days. Besides, the addition of TiO2 not only further improved the biocompatibility of the sample, but also endowed it with excellent bacteriostasis, which was expected to meet the standard of clinical implantation.

口腔疣状黄瘤(oral verruciform xanthoma,OVX)是一种罕见的口腔良性病变.本文的目的是报道我院近期的1例临床病例,并复习文献中报道的口腔疣状黄瘤病例,为临床诊疗提供参考.

Periodontitis is a chronic inflammatory disease that is considered to be the main cause of adult tooth loss. Diabetes mellitus (DM) has a bidirectional relationship with periodontitis. Interleukin-1β (IL-1β) is an important pre-inflammatory factor, which participates in the pathophysiological process of periodontitis and diabetes. The interleukin-1 receptor antagonist (IL-1ra) is a natural inhibitor of IL-1, and the balance between IL-1ra and IL-1β is one of the main factors affecting chronic periodontitis (CP) and diabetes. The purpose of this study is to develop a drug carrier that is safe and nontoxic and can effectively release IL-1ra, which can effectively slow down the inflammation of periodontal tissues with diabetes, and explore the possibility of lowering the blood sugar of this drug carrier. Therefore, in this experiment, a temperature-sensitive hydrogel loaded with IL-1ra was prepared and characterized, and its anti-inflammatory effect in high-sugar environments in vivo and in vitro was evaluated. The results showed that the hydrogel could gel after 5 min at 37 °C, the pore size was 5–70 μm, and the cumulative release of IL-1ra reached 83.23% on the 21st day. Real-time polymerase chain reaction (qRT-PCR) showed that the expression of IL-1β, Interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) inflammatory factors decreased after the treatment with IL-1ra-loaded thermosensitive hydrogel. Histological evaluation and micro-computed tomography (Micro-CT) showed that IL-1ra-loaded thermosensitive hydrogel could effectively inhibit periodontal inflammation and reduce alveolar bone absorption in rats with diabetic periodontitis. It is worth mentioning that this hydrogel also plays a role in relieving hyperglycemia. Therefore, the temperature-sensitive hydrogel loaded with IL-1ra may be an effective method to treat periodontitis with diabetes.

消退素是一种新型的内源性抗炎和促炎性消退脂质介质,在多种疾病模型中显示出其强效的生物学作用.有研究发现,消退素在预防和治疗牙周炎方面是有效的,具有良好的临床应用前景.本文就消退素在抗炎方面的作用及在牙周炎中的应用展开论述.

通过对1例牙龈瘤患者的病例资料及文献进行回顾分析,阐述规范化的牙周基础治疗联合手术切除牙龈瘤是治疗牙龈瘤并防止复发的有效手段,该病例治疗后慢性牙周炎基本控制,术区患牙均无明显松动,咀嚼功能良好,骨缺损区完全并无复发迹象,牙龈形态良好.阐明在牙龈瘤治疗过程中,彻底去除牙石、菌斑等局部刺激因素与牙龈瘤的治疗效果密切相关,患者自身的菌斑控制及牙周维护期的治疗更是防止牙龈瘤复发的关键.

以五味子叶为原料,对五味子叶保健饮料开发工艺进行了优化.通过提取温度、提取时间和料液比3个因素优化浸提条件,再根据不同比例调配并进行感官评价得到最佳的五味子叶保健饮料的工艺参数,即五味子叶粉末与水比例为1∶200 (g/mL),100℃水浸提1min获得五味子叶汁,再通过添加蜂蜜10％,VC 0.01％,柠檬酸0.05％最终获得五味子叶保健饮料产品.生产出的饮料具有独特的风味,且酸甜适口、营养丰富、组织状态良好,是具有开发前景的复合功能性饮料.

A water shedding carbon onion/carbon nanotube@Fe3O4 nanocomposite film prepared using a flame strategy possesses self-cleaning and microwave absorption properties.

The extraction condition of pectin from Aloe vera barbadensis Mill was optimized by a Box-Behnken design. The effect of parameters of extraction water proportion (EWP), extraction pH (EpH), extraction temperature (ETe), extraction time (ETi), alcohol precipitation pH (APpH)and alcohol precipitation temperature (APTe) on the extraction yield of pectin was investigated by a software of Design Export 8.0.5b. The maximum extraction yield was obtained with the EWP of 20:1, EpH of 1.5, ETe of 90 degrees C ETi of 120 min, APpH of 3.0 and APTe of 50 degrees C, which was consistent with the experimental value. We also found out that the pectin content decreased gradually during storage and sucrose concentration had a significant impact on the viscosity of pectin. (C) 2014 Elsevier Ltd. All rights reserved.