Impaired wound healing is associated with hyperglycaemia in patients with diabetes. Hyperglycaemia induces protein glycation and the formation of Advanced Glycation End-Products (AGEs). The accumulation of AGEs in the body results in the structural and functional modification of tissue proteins. This study was conducted to evaluate compounds with antiglycation activities (S-Ally1 Cysteine (SAC), N-Acetylcysteine (NAC) and the mimic compound A). The extent of glycation in the presence and absence of several inhibitors was assessed via several methods including fluorescence, Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)- silver stain, Western blotting, and Enzyme-Linked Immunosorbent Assays (ELISA). Additionally, this research aimed to evaluate and quantify the potential of Human Adipose Mesenchymal Stem Cells (hADMSCs) to uptake and release these drugs as potential therapeutics. To achieve this, hADMSCs were primed with a combination of SAC/NAC and mimic compound A and their concentrations were analysed using High-Performance Liquid Chromatography (HPLC). The SAC/NAC and mimic compound A prohibit the formation of AGEs while the Conditioned Medium (CM) from SAC/NACand compound A-loaded hADMSCs induced cell migration and tube formation in BAECs. hADMSCs provide a unique opportunity for the development of an innovative targeting and drug-delivery system which could effectively deliver therapeutics to specific regions of wounds or other damaged tissues. The data provided demonstrate the potential of hADMSCs as a drug delivery method with the potential to improve wound healing, and it may offer potential therapeutic targeting for the development of diabetic complications.

Advanced Glycation End-Products (AGEs) have been implicated in the chronic complications of Diabetes Mellitus (DM) and play an important role in the pathogenesis of atherosclerosis in human arteries. The main active compounds found in Aged Garlic Extract (AGExt) are S-Allyl Cysteine (SAC), N-Acetylcysteine (NAC) and S-allyl Mercaptocysteine (SAMC) and have powerful protective effects against oxidative stress and inhibit cellular damage by AGEs. Aims: To explore the effect of AGExt on the development of atherosclerosis also investigated the endothelial protective effects of AGExt, using Human Coronary Artery-Derived Endothelial Cells (HCAEC). The use of an optimised mixture of the above compounds required to obtain and provide maximal beneficial effects against oxidative stress, cell apoptosis and protein glycation. Results: AGExt has protective effects against the cellular damage of HCAEC. In addition, the in vivo study accessed the activity of SAC and NAC to reduce the severity of atherosclerosis formation in Apolipoprotein (ApoE-/-) DM model mouse. The compounds of AGExt in vitro had strong protective actions against the AGEs induced damage to ECs even at low concentrations (100 ng/ml). Such low concentrations may have therapeutic usefulness in patients with diabetes.

AIM:To explore the clinical value of magnetic resonance imaging (MRI) combined with serum prostate specific antigen (PSA), epithelial cadherin (sE-cadherin) and early prostate cancer antigen-2 (EPCA-2) in prostate cancer (PC) diagnosis.PATIENTS AND METHODS:Fifty patients with PC and 50 with benign prostatic hyperplasia (BPH) confirmed by pathology from January 2020 to July 2021 were studied retrospectively. All patients underwent MRI and measurement of the serum levels of PSA, EPCA-2, and sE-cadherin. The diagnostic accuracy and efficacy of these methods was compared between the groups.RESULTS:In MRI diagnosis of PC, lesions were mainly located in the peripheral zone; T2-weighted imaging of this zone showed low signal intensity, with different degrees of prostate enlargement. BPH had a clear boundary, complete capsule and central zone hyperplasia and uneven signal nodules. PC and BPH had different degrees of prostate enlargement. Serum levels of PSA, sE-cadherin and EPCA-2 in the cancer group were significantly higher than those in the BPH group (p<0.05). The diagnostic concordance of combined assessment of MRI, PSA, sE-cadherin, and EPCA-2 in differentiating PC from BPH was 93%, which was significantly higher than these approaches used alone (84%, 79%, 81% and 82%, respectively; p<0.05). The area under the receiver operating characteristics curve for the combined approach in PC diagnosis was 0.900, which was significantly higher than those for the individual methods (0.840, 0.730, 0.760 and 0.810, respectively; Z=2.343, p=0.004).CONCLUSION:MRI combined with PSA, sE-cadherin and EPCA-2 can improve the sensitivity and accuracy of PC diagnosis and has potential as a guiding scheme for early diagnosis of PC.

目的:分析儿科护理管理中的风险因素及应对措施。方法:本次实验中共选取研究对象120例，均为儿科患者，患有不同病症，符合实验入组标准，并且在入组后，根据护理措施的不同将其分配为对照组和观察组，每组60例，分别使用常规护理、风险管理，对比其风险事件发生率，找出导致儿科护理管理工作中引发护理风险事件的风险因素。结果:观察组中出现2例风险事件，对照组出现14例，风险事件发生率分别为3.33%、23.33%，观察组明显更低，（P＜0.05）。而且主要因患儿因素、护理人员因素、医院管理因素导致。结论:应针对患儿、护理

目的:研究分析血透室护理质量管理中流程管理的作用，为护理人员改进后续血透室护理工作方案带来一些帮助?方法:选择血透室患者进行研究，本次实验时间为2019年8月至2021年2月这一时间段，共计54例血透室患者参与实验中来?将随机编号的结果作为划分血透室患者的依据，对照组患者共计27人，均为奇数，接受常规管理模式，实验组27人为偶数编号，接受流程管理模式，记录对照组及实验组患者对护理工作所持态度?不良事件出现情况?治疗顺应情况?相关知识掌握评分，并加以比较分析?结果:对照组患者中对护理工作持十分认可及比较认可

Takayasu动脉炎(TA)是累及主动脉及其分支的特发性慢性血管炎,可造成相应部位血管的狭窄乃至闭塞,从而引起局部缺血症状.但其发病初期临床特征不典型,难以评估疾病活动,疾病诊断依旧是一个重大难题.在治疗方面,药物主要基于皮质类固醇、免疫抑制剂和生物制剂,当疾病较重或合并相关并发症致保守治疗无效时,需要手术治疗,其中创伤小的微创手术表现出了广阔的应用前景.本文主要概括了TA的病因、临床表现、目前诊断标准、影像学检查结果、实验室检查潜在的标志物和现今的治疗方式,以期为该病的诊断治疗提供指导.

目的:探讨人格特质及应对方式对医学生心理健康的影响.方法:采用分层整群抽样法对潍坊医学院486名医学生进行艾森克人格问卷简式量表(EPQ-RSC)、简易应对方式问卷(SCSQ)及一般健康问卷(GHQ-20)调查研究,采用通径分析研究影响医学生心理健康的主要因素及其作用方式.结果:研究显示,外向型和神经质人格是医学生自我肯定的影响因素;精神质和神经质人格是忧虑的影响因素;精神质、神经质和外向型人格是焦虑的主要影响因素.积极应对方式对自我肯定和忧虑有影响;消极应对方式仅对自我肯定有影响.4种人格特质中,神经质和精神质人格对心理健康的影响较大..结论:不同人格特质对医学生心理健康有直接影响,也可通过应对方式对个体心理健康产生间接影响.应对方式对医学生心理健康有直接影响作用.

Circular RNAs (circRNAs) and microRNAs (miRNAs) have been emerging as new players in acute myeloid leukemia (AML). Hsa_circ_0005774 (circ_0005774) is an upregulated circRNA in pediatric AML, while its role is uncovered. Thus, we intended to measure the function and mechanism of circ_0005774 in AML leukemogenesis. Real time-quantitative PCR revealed that circ_0005774 was highly expressed in blood of pediatric AML patients and AML cells (HL-60 and NB4), accompanied with downregulated miRNA-192–5p (miR-192–5p) which was a crucial tumor-associated and leukemia-related miRNA. Circ_0005774 was abundant in miRNA response element according to CSCD software, and miR-192–5p was identified as a target of circ_0005774, as evidenced by RNA immunoprecipitation and dual-luciferase reporter assays. Cell viability assay, flow cytometry and western blotting were performed to measure cell functions. Accordingly, blocking circ_0005774 and/or overexpressing miR-192–5p could enhance apoptosis rate of HL-60 and NB4 cells, but suppress cell viability and cell cycle entrance, accompanied with depression of proliferation markers including proliferating cell nuclear antigen (PCNA), CyclinD1 and B cell lymphoma 2 (Bcl-2). Meanwhile, depleting miR-192–5p counteracted the role of circ_0005774 knockdown in AML cells. Uncoordinated 51-like kinase 1 (ULK1) was previously demonstrated to be associated with diagnosis, prognosis and therapeutic strategy for AML, and restoring ULK1 could abrogate miR-192–5p overexpression-induced effects in HL-60 and NB4 cells. Notably, ULK1 was a downstream target of miR-192–5p and indirectly modulated by circ_0005774. In conclusion, circ_0005774 knockdown repressed cell proliferation and promoted apoptosis of AML cells partially through regulating miR-192–5p/ULK1 axis.

1Department of Interventional Thoracic Oncology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, 261031, People’s Republic of China; 2Department of Hematology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, 261031, People’s Republic of China; 3Department of Pediatrics, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, 261031, People’s Republic of China Purpose: MicroRNA-4284 (miR-4284) was demonstrated to be aberrantly expressed and affected cell activities in some types of diseases, including cancer. However, the role of miR4284 in non-small cell lung cancer (NSCLC) is largely unknown. The aim of this study was to investigate the expression and biological role of miR-4284 in NSCLC. Patients and Methods: The qRT-PCR assay was applied to detect the expression of miR4284 in NSCLC tissues and cell lines. Kaplan–Meier curve method and multiple Cox regression analyses were used to explore the prognostic factors for postoperative NSCLC patients. The CCK-8 assay was carried out to measure the proliferative abilities of A549 and H1299 cells. Transwell migration and invasion assays were used to determine the cell migratory and invasive capabilities of NSCLC cells. Results: miR-4284 expression was upregulated in NSCLC tissues and cell lines. High expression of miR-4284 was correlated with poor differentiation, positive lymph node metastasis, and advanced TNM stages. In addition, postoperative patients with higher expression of miR-4284 exhibited a shorter overall survival time than those with lower expression of miR-4284. Moreover, the upregulation of miR-4284 accelerated cell proliferative, migratory, and invasive abilities of A549 and H1299 cells, while the downregulation of miR-4284 inhibited these cellular capabilities. Conclusion: miR-4284 was noticeably upregulated in NSCLC and associated with a poor prognosis of postoperative NSCLC patients. miR-4284 promoted the proliferation, migration, and invasion of A549 and H1299 cells. This study indicated that miR-4284 might serve as a prognostic biomarker and a potential therapeutic target for postoperative NSCLC patients.

PURPOSE:MicroRNA-4284 (miR-4284) was demonstrated to be aberrantly expressed and affected cell activities in some types of diseases, including cancer. However, the role of miR-4284 in non-small cell lung cancer (NSCLC) is largely unknown. The aim of this study was to investigate the expression and biological role of miR-4284 in NSCLC.PATIENTS AND METHODS:The qRT-PCR assay was applied to detect the expression of miR-4284 in NSCLC tissues and cell lines. Kaplan-Meier curve method and multiple Cox regression analyses were used to explore the prognostic factors for postoperative NSCLC patients. The CCK-8 assay was carried out to measure the proliferative abilities of A549 and H1299 cells. Transwell migration and invasion assays were used to determine the cell migratory and invasive capabilities of NSCLC cells.RESULTS:miR-4284 expression was upregulated in NSCLC tissues and cell lines. High expression of miR-4284 was correlated with poor differentiation, positive lymph node metastasis, and advanced TNM stages. In addition, postoperative patients with higher expression of miR-4284 exhibited a shorter overall survival time than those with lower expression of miR-4284. Moreover, the upregulation of miR-4284 accelerated cell proliferative, migratory, and invasive abilities of A549 and H1299 cells, while the downregulation of miR-4284 inhibited these cellular capabilities.CONCLUSION:miR-4284 was noticeably upregulated in NSCLC and associated with a poor prognosis of postoperative NSCLC patients. miR-4284 promoted the proliferation, migration, and invasion of A549 and H1299 cells. This study indicated that miR-4284 might serve as a prognostic biomarker and a potential therapeutic target for postoperative NSCLC patients.

目的 提高强直性脊柱炎居家患者功能锻炼效果.方法 将门诊就诊的强直性脊柱炎患者67例采用随机数字表法分为对照组34例、观察组33例.对照组依据功能锻炼方案进行康复训练,每周5次,每次30 min;观察组依据功能锻炼方案实施基于碎片化时间的功能锻炼.干预3个月后,比较两组功能锻炼依从性、强直性脊柱炎功能指数及晨僵、疼痛、枕墙距、胸廓活动度的改善情况.结果 干预后,观察组功能锻炼依从程度显著高于对照组,且观察组晨僵、枕墙距、胸廓活动度及强直性脊柱炎功能指数改善程度显著优于对照组(均P＜0.05).结论 对强直性脊柱炎居家患者实施基于碎片化时间的功能锻炼,有助于提高患者功能锻炼依从性,改善患者症状及脊柱功能,提高日常生活功能.

目的 探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)长链非编码RNA(lncRNA)ENST00000604411.1的表达变化及意义.方法 选择SLE患者30例,根据SLE疾病活动度(SLEDAI)评分将患者分为活动组18例(SLEDAI评分≥9分)、缓解组12例(SLEDAI评分<9分),同期选体检健康者20例作为健康对照组.用Ficoll梯度密度原理分离纯化PBMC,用生物芯片技术筛选SLE患者差异表达lncRNA,用实时荧光定量PCR法检测PBMC中LncRNA ENST00000604411.1表达;用魏氏法检测血沉(ESR),用速率散射比浊法检测补体C3.用Pearson线性相关分析指标间的相关性.结果 共筛选出163个差异表达lncRNA,其中表达上调基因118个,表达下调基因45个.选择表达差异明显的lncRNA ENST00000604411.1作为观察指标.SLE组、正常对照组PB-MC中lncRNA ENST00000604411.1相对表达量分别为15.10±1.46、3.69±0.95,比较差异有统计学意义(P<0.05).与健康对照组比较,缓解组、活动组ESR水平高、补体C3水平低(P均<0.05);与缓解组比较,活动组ESR水平高、补体C3水平低(P均<0.05).Pearson相关分析结果显示,SLE患者PBMC中lncRNA ENST00000604411.1表达与ESR、SLEDAI均呈正相关(r分别为0.563、0.524,P均<0.05),但与补体C3呈负相关(r=-0.618,P<0.05).结论 SLE患者PBMC中lncRNA ENST00000604411.1表达上调,其表达变化在一定程度上可反映疾病活动度.

目的 探讨认知功能干预对系统性红斑狼疮(SLE)患者认知功能及日常生活活动能力的影响.方法 选取2016年8月～2018年2月我院收治的46例伴有认知功能障碍的SLE患者作为研究对象,随机分为干预组与对照组,每组各23例.干预组采用每周3次、每次2h的认知功能训练方式进行认知功能干预,对照组不进行任何认知功能干预.比较两组患者3个月后的蒙特利尔认知量表(MoCA)、基本认知能力测验表及日常生活活动能力(ADL-BI)评分.结果 干预后,干预组的MoCA量表评分(除语言与抽象子项)、ADL-BI评分均高于干预前和对照组,差异有统计学意义(P＜0.05);干预后,干预组的各项基本认知能力测验评分均高于干预前和对照组,差异有统计学意义(P＜0.05).结论 认知功能干预可以改善SLE患者的认知水平和日常生活活动能力.

Objective To investigate the cognitive impairment and risk factors in patients with systemic lupus erythematosus (SLE). Methods 65 patients with systemic lupus erythematosus were diagnosed in the Affiliated Hospital of Weifang Medical College, from January 2015 to January 2019, including 12 males and 53 females, with an average age of (32.5±13.5) years and an average length of education of (13.6±5.4) years. Sixty-five patients with non-systemic lupus erythematosus hospitalized in our hospital during the same period were selected as control group, 12 males. Fifty-three women, with an average age of (35.5+15.5) years and an average length of education of (12.5+6.5) years, were assessed by Montreal Cognitive Function Scale (MoCA) and their risk factors were analyzed by Logistic. Results Among 65 SLE patients, 32 (49.23％) had cognitive impairment, which was significantly higher than 15 (23.08％) in the control group.Compared with the MoCA scale of the control group, the scores of visual space and executive ability, naming, attention, abstraction, delayed recall, orientation and total score of SLE group were significantly lower than those of the control group. Logistic analysis showed that the course of disease was long (OR 2.01, 95％ CI 1.52-5.84), combined with nervous system damage (OR 5.92, 95％ CI 1.56-8.34), kidney disease (OR 3.45, 95％ CI 1.34-4.72), hematological complications (OR 3.73％, 95％ CI 1.05-5.68), and side effects of drugs were significant (OR 2.92, 95％ CI 1.56-8.34), and hematological complications (OR 3.73％, 95％ CI 1.05-5.68), insufficient social security (OR 1.45, 95％ CI 1.04-4.72), and long time to achieve clinical remission (OR 2.73, 95％ CI 1.05-3.68) are independent risk factors for cognitive impairment in SLE patients. Conclusion Systemic lupus erythematosus patients with high incidence of cognitive impairment, long course of disease, combined with nervous system damage, kidney disease, blood system complications, significant side effects of drugs, inadequate social security, long time to achieve clinical remission are independent risk factors for cognitive impairment of systemic lupus erythematosus.

In this study, the protective effect of valsartan against glycerol-induced acute kidney injury (AKI) in male albino rats was investigated. Valsartan is used to treat high blood pressure and congestive heart failure and can prolong lifespan following a heart attack. The rats were divided into control, AKI, AKI + valsartan 100 mg/kg bw, and AKI + valsartan 200 mg/kg bw groups. Superoxide dismutase, glutathione peroxidase, catalase, lipid peroxidation, and reduced glutathione were assessed, and histopathological, immunohistochemical and western blot analyses were performed. Valsartan supplementation in AKI rats substantially increased superoxide dismutase, catalase, glutathione peroxidase, and glutathione levels but reduced the level of lipid peroxidation. Valsartan significantly reduced the severity of the renal tubular injury, renal lesions, and necrosis. Valsartan decreased NF-κB and TLR4 mRNA expression by >50% and their protein levels by >40%. Therefore, valsartan supplementation inhibited glycerol-induced functional and pathological damage to the kidney in a concentration-dependent manner. We propose that valsartan protects rat kidney tissue by downregulating NF-κB and TLR4 expression.

The aim of this study was to explore the correlation of salazosulfamide efficacy on ankylosing spondylitis and N-acetyltransferase 1 (NAT1) gene polymorphism. Thirty-two patients with ankylosing spondylitis were recruited in the experimental group and 36 normal individuals were recruited to the control group. The experimental group received 8.0 mg of salazosulfamide (MTX) per week and the control group received isodose of normal saline. Twenty-six patients in the experimental group responded to the salazosulfamide treatment and 6 did not show response. Morning stiffness time of patients in the experimental group who responded to salazosulfamide was significantly lower than that of patients with no reaction to salazosulfamide, and similar to patients in the control group. The average tender joint count of patients in the experimental group that responded to salazosulfamide was lower than in patients with no response to treatment, and similar to patients in the control group. NAT1 gene sequencing determined that the patients sensitive to salazosulfamide treatment manifested as AA/AG at 263 locus, whereas patients not sensitive to salazosulfamide were GG. NAT1 expression was comparable between the different genotypes at the mRNA level. However, there was a significant difference of NAT1 protein between groups. Overall, salazosulfamide demonstrates curative activity for ankylosing spondylitis and we believe that NAT1 AA/GG genotype at 263 locus can promote salazosulfamide effectiveness on ankylosing spondylitis.

目的 评价自拟扶正祛疣汤治疗尖锐湿疣临床疗效.方法 96例患者随机分为治疗组和对照组,2组患者均采用CO2激光祛除疣体,创面外用龙珠软膏;治疗组每日口服自拟扶正祛疣汤,并局部熏洗,20 min/次,2次/d,连用1个月.治疗后每月进行随访,治疗结束后3个月判定痊愈率和复发率.结果 治疗组痊愈率优于对照组,复发率低于对照组(P＜0.05).结论 扶正祛疣汤治疗尖锐湿疣可提高疗效,降低复发率,不良反应小.

目的评价自拟扶正祛疣汤治疗尖锐湿疣临床疗效。方法 96例患者随机分为治疗组和对照组,2组患者均采用CO2激光祛除疣体,创面外用龙珠软膏;治疗组每日口服自拟扶正祛疣汤,并局部熏洗,20 min/次,2次/d,连用1个月。治疗后每月进行随访,治疗结束后3个月判定痊愈率和复发率。结果治疗组痊愈率优于对照组,复发率低于对照组(P<0.05)。结论扶正祛疣汤治疗尖锐湿疣可提高疗效,降低复发率,不良反应小。

目的:探讨早产儿开始胃肠喂养的时间。方法:将89例早产儿随机分为早开奶组47例(≤3d)和晚开奶组42例(>3d),用同一配方奶喂养,记录早产儿住院天数、喂养不耐受情况、达足量喂养的时间。结果:早开奶组住院天数、喂养不耐受比例、达足量喂养时间明显少于晚开奶组,两组比较有显著性差异(P<0.05)。结论:对早产儿尽可能早期开始胃肠喂养,有利于胃肠功能恢复,缩短住院时间,减少并发症。

Objective To investigate the changes and its clinical significance of the myocardial zymogram of infant with pneumonia.Methods 66 cases were devided into two groups:the common group and serious group.Test their serum myocardial zymogram after hospitalization,and test the serious group again after 1 week's treatment.Results Among 66 cases,serum myocardial zymogram increased in 48 patients,and the 18 cases whose serum myocardial zymogram was normal were all in common group;in the serious group,the number of the infants whose serum myocardial zymogram increased and the level of changes of one or more items of the myocardial zymogram were all higher than those of the common group(P0.01);after treatment in serious group,the recorery of CK was the fastest,and then were the CK-MB,the LDH,the AST.Conclusion Pneumonia can cause the changes of the serum myocardial zymogram.With the progress of the patient's condition,the myocardial injury is increasingly obvious.This suggests:for the infants with pneumonia,if we want to cuel them more quickly,we must protect the myocardium besides the regular therapy.