介绍一例闭锁型犬子宫畜脓症的发病症状,分析其病因与病理生理学,提出诊断和治疗方法,以为该病的诊治提供参考。

To explore effects of micrurgy and tissue explants adhension to culture vascular endothelial cells from albino rat aorta.The cell growth was evidenced by revealing the morphological characteristics under inverted phase contrast microscope and detection of Ⅷ factor(or von Willebrand factor,vWF)by immunocytochemistry.The results showed that the migration of endothelial cells from aortic cub was found after 72h's cultivation,forming confluent monolayer of a cobblestone pattern after 12 days.Cell positive rate of vWF in the cytoplasm of the new cells was 97%.Combination micrurgy with tissue explants adhension could obtain pure vascular endothelial cells.

Folium perillae injection and chick embryo fibroblast were cultured together in vitro to determine the maximum safe concentration of Folium perillae injection and its cytotoxicity in chick embryo fibroblast culture system.The results showed that the maximum safe concentration of Folium perillae injection was 488.281μg/mL.

Objective To evaluate the compatible stability of Rhubarb Injection with Folium Perillae Injection at room temperature. Methods After Folium Perillae injection and Rhubarb injection were prepared,quality control was conducted according to the"Pharmacopeia of China 2005". Appearance,absorbability value,pH value and particles of mixture of the two injections within 6h were detected by ultraviolet-visible spectrophotometer,pH meter and microscope. Results No significant change was observed in appearance,particles,pH value and absorbability value of the mixture at room temperature. Conclusion Rhubarb injection mixed with Folium Perillae injection has good stability and the two injections can be mixed to use.

结石在膀胱形成,随尿液进入尿道而使尿道阻塞时,犬会并发尿毒症.当尿道被结石完全阻塞时,手术治疗是快速恢复尿路通畅的最有效的治疗手段.小型犬发生尿道膀胱结石时,因其体型偏小,尿道窄小,手术治疗难度较大.笔者于2009年5月31日接诊1例西施犬尿道膀胱结石病例,经手术治疗后已完全康复.

Objective Intimal hyperplasia after bypass surgery is closely related with the flow pattern in bypassed artery,which is the main cause of surgery failure.In order to improve the flow pattern structure and offer an optimization design,an axis-deviated arterial bypass(ADAB) methods was designed and computational numerical simulation of flow patterns was conducted.Methods Sixteen dogs were randomly divided into two groups:the axis-deviated arterial bypass group and conventional arterial bypass(CAB) group.Geometry data of the bypassed vessels were measured before and after surgery.Blood pressure and blood flow volume at the proximal and distal anastomosis were determined after bypass surgery.Flow structures in the bypassed vessels were simulated using FLUENT 6.2 software.Results The proximal and distal anastomosis was not in the same plane in ADAB group.In CAB group,the vortex flow along the floor of the host artery opposite the anastomotic junction turns into two symmetrical helical structures that possess the typical characteristics of the Dean-type flow pattern.There exists a rather low wall shear stress area around the flow stagnation point that locates approximately at the middle of the host artery floor opposite the bypass junction.In ADAB group,an apparent swirling flow occurs immediately when the fluid enters the host artery and moves along the floor of the host artery with its strength intact.The swirling flow created in axis-deviated bypass model significantly altered the overall flow pattern in the host artery,eliminated the low wall shear stress zone and enhanced the wall shear stress along the floor of the host artery.Axis-deviated arterial bypass could induce a swirling flow in the bypassed host artery.Conclusion Axis-deviated arterial bypass may create a swirling flow,increasing the blow flow volume and wall shear stress in the distal anastomoses.

为了研究激素冲击疗法对肺炎型犬瘟热病犬的血常规的影响,试验采用常规疗法的同时给患犬静脉注射地塞米松2mg/kg行激素冲击疗法,并于试验前和每天用药后4小时采血,用CA-500血液分析仪测定白细胞（WBC）数、淋巴细胞（LYM）数、中间细胞（MON）比率、红细胞（RBC）数、血红蛋白（HGB）含量、血小板（PLT）数等指标,以治疗前测定值为基础值,观察激素冲击疗法后病犬血常规的变化。结果表明:对照组病犬WBC数、MON比率无显著变化,LYM数在治疗结束时极显著降低（P＜0.01）。试验组WBC在用药后2天就显著升高（P＜0.05）,第3天极显著升高（P＜0.01）;LYM在用药后1天就显著升高（P＜0.05）;其他参数如红细胞、血小板无显著变化。

1 发病情况寻血犬,8岁,体重28 kg,未曾生育过.2009年3月初发情,发情时主人见有褐色分泌物从阴道流出,开始食欲不振,发情约一周后不吃,但饮欲明显增强.于2009年3月19日前来求诊,就诊时鼻镜干,精神较差.

To investigate the effects of Rhubarb injection on fibrinolytic system after vascular bypass surgery,twenty hybridization dogs were divided to four groups randomly,which were control,high dosage(0.8 g·kg-1),middle dosage(0.4 g·kg-1) and low dosage group(0.08 g·kg-1).The common carotid artery was reconstructed with jugular vein from the same side.Administration of Rhubarb injection was intravenous transfusion through vena cephalica accessoria.Parameters of fibrinolytic system was detected by ELISA quantitative assay before operation,1,2,3,5,7,14,21 d postoperatively.Results were as follows:(1) Granule membrane protein 140,D-dimer,plasminogen activator inhibitor-1 was significantly reduced;(2) Tissue-type plasminogen activator was significantly increased;(3) Fibrinogen degradation products had no change.Results showed that Rhubarb injection could retrieve blood hypercoagulable state of fibrinolytic system,recovery activity of anticoagulation system and prevent thrombogenesis after vascular bypass surgery.

阴道内膜增生指远端阴道黏膜腹侧壁水肿、增生,并向后脱出于阴门内或阴门外.主要见于发情前期和发情期的年轻母犬.

Objective: To explore the effects of Folium Perillae injection on blood parameters after vascular bypass surgery in dogs.Method: Twenty hybridization dogs were randomly divided into high,middle,low dosage groups(1 434 mg/kg,478 mg/kg,159 mg/kg)and control.The common carotid artery was reconstructed with jugular vein from the same side by 45° bypass vascular surgery.Folium Perillae injection was intravenously administrated from brachiocephalic vein daily for 3 weeks.The blood parameters were detected at the 0,1,2,and 3 weeks after drug administration.Results: Compared with the control group,low,medium and high dosage of Folium Perillae injection could significantly decrease the whole blood viscosity and the whole blood reductive viscosity at low shear rate(10 s-1),erythrocyte aggregation index and erythrocyte electrophoresis index(P<0.05 or P<0.01).High dosage of Folium Perillae injection could significantly decrease the whole blood viscosity and the whole blood reductive viscosity at middle shear rate(60 s-1)(P<0.05),but low and middle dosage of Folium Perillae injection had no significant effects on the whole blood viscosity and the whole blood reductive viscosity at medium shear rate(60 s-1)(P>0.05).Low,middle and high dosage of Folium Perillae injection had no significant effects on hematocrit,plasma viscosity,erythrocyte rigidity index,the whole blood viscosity and the whole blood reductive viscosity at high shear rate(150 s-1).Conclusions: Folium Perillae injection could significantly improve the hemorrheological characteristics of dogs after vascular bypass surgery.

Objective To investigate the effects of ePTFE cuff on arterialization of vein after bypass surgery in dogs. Methods Thirty-six healthy dogs were randomly divided into 2 groups ( n = 18 in each group). The implanted veins in experimental group were enclosed by ePTFE, and that of the control group were not enclosed. Common carotid artery was reconstructed with ipsilateral external jugular vein. Animals were sacrificed at 2, 6 and 10w postoperatively, respectively. The vein graft was separated and using pressure perfused by formalin. The explanted vein was cut at the middle, then the proximal segment was divided into two segments, the segment near the anastomosis was as "a" segment, the other was " b" segment. Segments a and b were sliced into 3 mm sections, respectively. Thickness of the vascular wall was analyzed and measured. Results In control group, basic value of lateral wall thickness of the a segment was (0. 46 ± 0.09) mm, and it increased to (0. 64 ±0.06) ,( 2.20 ±0.51 ),( 3.23±0. 3 8 ) mm, respectively, at 2 , 6 and 10w. Basic value of medial wall thickness of the b segment was ( 0. 46 ± 0. 09 ) mm, whichincreased to( 0. 59 ±0.05) , ( 2. 46 ±0. 41) , ( 2. 01 ±0. 11) mm, respectively at 2 , 6 and 10w. In experimental group, basic value of the lateral wall thickness of the a segment was (0.41 ±0.09) mm, and the value was (0. 75 ± 0. 22 ) , ( 1. 31 ± 0. 21 ) mm, respectively, at 6 and 10w. Basic value of the medial wall thickness of the b segment was (0.41 ± 0. 09 ) mm, which was ( 0. 70 ± 0. 04 ) , ( 1. 24 ± 0.28) mm, respectively, at 6 and lOw. Compared with the control group, the vessel wall thickness in experimental group was decreased significantly ( P ＜ 0. 01 ) . Conclusions ePTFE cuff can retard the arterialization process of implanted vein.

为了研究出现典型临床症状犬瘟热病犬肝功能的变化,为犬瘟热的治疗及预后提供理论指导,试验采用半自动生化分析仪测定了5例犬瘟热病犬丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)及血清总蛋白(TP)、白蛋白(ALB)、球蛋白(SP)、总胆红素(T-Bil)、直接胆红素(D-Bil)等指标.结果表明:出现典型临床症状犬瘟热病犬的ALT、AST普遍偏高,测定值分别为(84.20±17.68) U/L、(79.20±14.14) U/L;TP、ALB普遍偏低,分别为(53.00±7.31) g/L、(24.80±5.17) g/L;T-Bil、D-Bil、SP浓度分别为(5.96±1.47)μmol/L、(3.88±1.84) μmol/L 和(28.30±8.26) g/L,变化不明显.

Objective:To observe the effects of different bypass vascular anastomosis methods on blood flow,blood pressure,and intimal hyperplasia at the opposite site of distal stoma.Methods:Sixteen dogs were randomly divided into two groups:eccentric bypass grafting group(group EBG)and conventional bypass grafting group(group CBG).Blood pressure and blood flow volume at proximal and distal stoma were determined after bypass surgery.Pathological changes were observed with tissue sections and vascular intimal thickness of distal stoma was determined in 3 months after the surgery.Results:(1)The blood flow volume of EBG group(2.7±0.4)at distal stomas were significantly higher(P0.01)than those of group CBG(2.1±0.5 mL/s);(2)Systolic blood pressure and diastolic blood pressure at distal stoma in group EBG(97±19 and 86±17 mmHg)were higher than those of group CBG(96 ±14 and 76±14 mmHg),but the difference was not significant statistically;(3)The intimal thickness were 110.50±9.62 μm and 60.32±3.52 μm in CBG group and EBG group,respectively.The difference was statistically significant(P0.01).Conclusions:The eccentric bypass method could inhibit intimal hyperplasia at the opposite site of distal stoma by enhancing blood flow volume.

Objective:Study on the effect of Polygonum multiflo-rum Thunb(PmT) on blood lipid and fatty Liver of Rabbit with Hyperlipidemia.Method:The high-lipid diet was fed to establish the Hyperlipidemia model of rabbit.The normal control group,hyperlipidemia model group,high does of PmT group,middle does of PmT group,low does of PmT group were designed to measure the contents of total cholesterol(TC),three acids glyc-eride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),Experimental observation of the eighth week of liver histopathology.Result: All the test groups could decrease TC,TG,LDL-C content(P<0.01) and increase the HDL-C content(P <0.05) in serum,The liver fat lesions of Polygonum multifl orum Thunb high and middle dose groups were reduced,Fatty change of liver cells was significantly decreased.Conclusion: Polygonum multiflo-rum Thunb is capable of regulating blood lipids and the role of inhibiting the formation of fatty liver.

选取10条雄性健康犬,随机分成对照组和治疗组,每组5条.实验动物模型采用同侧自体颈静脉行颈动脉45.搭桥.术后均采取常规治疗,治疗组给予肝素钠.分别于术前及术后1、2、3天在颈静脉采血,检测D-二聚体、血清纤维蛋白(原)降解产物(FDP)、血浆血小板a颗粒膜蛋白(GMP-140)、纤溶酶原激活剂的抑制物-1(PAI-1)和组织纤溶酶原激活剂(t-PA)等纤维蛋白溶解系统(纤溶)指标的变化.结果:治疗组与对照组相比,D-二聚体.GMP-140、PAI-1显著降低(P＜0.05),t-PA显著升高(P＜0.05),FDP变化不显著(P＞0.05).结论:肝素钠可改善血管搭桥术后纤溶系统,纠正血液高凝状态,恢复抗凝系统活性.