To explore effects of micrurgy and tissue explants adhension to culture vascular endothelial cells from albino rat aorta.The cell growth was evidenced by revealing the morphological characteristics under inverted phase contrast microscope and detection of Ⅷ factor(or von Willebrand factor,vWF)by immunocytochemistry.The results showed that the migration of endothelial cells from aortic cub was found after 72h's cultivation,forming confluent monolayer of a cobblestone pattern after 12 days.Cell positive rate of vWF in the cytoplasm of the new cells was 97%.Combination micrurgy with tissue explants adhension could obtain pure vascular endothelial cells.

Objective To evaluate the compatible stability of Rhubarb Injection with Folium Perillae Injection at room temperature. Methods After Folium Perillae injection and Rhubarb injection were prepared,quality control was conducted according to the"Pharmacopeia of China 2005". Appearance,absorbability value,pH value and particles of mixture of the two injections within 6h were detected by ultraviolet-visible spectrophotometer,pH meter and microscope. Results No significant change was observed in appearance,particles,pH value and absorbability value of the mixture at room temperature. Conclusion Rhubarb injection mixed with Folium Perillae injection has good stability and the two injections can be mixed to use.

Folium perillae injection and chick embryo fibroblast were cultured together in vitro to determine the maximum safe concentration of Folium perillae injection and its cytotoxicity in chick embryo fibroblast culture system.The results showed that the maximum safe concentration of Folium perillae injection was 488.281μg/mL.

犬耳血肿是在外力作用下耳部血管破裂、出血而形成的肿胀,感染后可形成脓肿,该病常见于大耳犬.临床上对于局限性小血肿采用保守疗法即可治愈,而对于较大血肿,保守疗法则难以奏效,往往采取手术疗法.此外对于兴奋性品种犬耳血肿,往往需要更加精心的护理才能使其预后良好,笔者近期治愈一例顽固性耳血肿,现介绍如下.

1 发病情况寻血犬,8岁,体重28 kg,未曾生育过.2009年3月初发情,发情时主人见有褐色分泌物从阴道流出,开始食欲不振,发情约一周后不吃,但饮欲明显增强.于2009年3月19日前来求诊,就诊时鼻镜干,精神较差.

阴道内膜增生指远端阴道黏膜腹侧壁水肿、增生,并向后脱出于阴门内或阴门外.主要见于发情前期和发情期的年轻母犬.

Objective: To explore the effects of Folium Perillae injection on blood parameters after vascular bypass surgery in dogs.Method: Twenty hybridization dogs were randomly divided into high,middle,low dosage groups(1 434 mg/kg,478 mg/kg,159 mg/kg)and control.The common carotid artery was reconstructed with jugular vein from the same side by 45° bypass vascular surgery.Folium Perillae injection was intravenously administrated from brachiocephalic vein daily for 3 weeks.The blood parameters were detected at the 0,1,2,and 3 weeks after drug administration.Results: Compared with the control group,low,medium and high dosage of Folium Perillae injection could significantly decrease the whole blood viscosity and the whole blood reductive viscosity at low shear rate(10 s-1),erythrocyte aggregation index and erythrocyte electrophoresis index(P<0.05 or P<0.01).High dosage of Folium Perillae injection could significantly decrease the whole blood viscosity and the whole blood reductive viscosity at middle shear rate(60 s-1)(P<0.05),but low and middle dosage of Folium Perillae injection had no significant effects on the whole blood viscosity and the whole blood reductive viscosity at medium shear rate(60 s-1)(P>0.05).Low,middle and high dosage of Folium Perillae injection had no significant effects on hematocrit,plasma viscosity,erythrocyte rigidity index,the whole blood viscosity and the whole blood reductive viscosity at high shear rate(150 s-1).Conclusions: Folium Perillae injection could significantly improve the hemorrheological characteristics of dogs after vascular bypass surgery.

Objective To investigate the effects of ePTFE cuff on arterialization of vein after bypass surgery in dogs. Methods Thirty-six healthy dogs were randomly divided into 2 groups ( n = 18 in each group). The implanted veins in experimental group were enclosed by ePTFE, and that of the control group were not enclosed. Common carotid artery was reconstructed with ipsilateral external jugular vein. Animals were sacrificed at 2, 6 and 10w postoperatively, respectively. The vein graft was separated and using pressure perfused by formalin. The explanted vein was cut at the middle, then the proximal segment was divided into two segments, the segment near the anastomosis was as "a" segment, the other was " b" segment. Segments a and b were sliced into 3 mm sections, respectively. Thickness of the vascular wall was analyzed and measured. Results In control group, basic value of lateral wall thickness of the a segment was (0. 46 ± 0.09) mm, and it increased to (0. 64 ±0.06) ,( 2.20 ±0.51 ),( 3.23±0. 3 8 ) mm, respectively, at 2 , 6 and 10w. Basic value of medial wall thickness of the b segment was ( 0. 46 ± 0. 09 ) mm, whichincreased to( 0. 59 ±0.05) , ( 2. 46 ±0. 41) , ( 2. 01 ±0. 11) mm, respectively at 2 , 6 and 10w. In experimental group, basic value of the lateral wall thickness of the a segment was (0.41 ±0.09) mm, and the value was (0. 75 ± 0. 22 ) , ( 1. 31 ± 0. 21 ) mm, respectively, at 6 and 10w. Basic value of the medial wall thickness of the b segment was (0.41 ± 0. 09 ) mm, which was ( 0. 70 ± 0. 04 ) , ( 1. 24 ± 0.28) mm, respectively, at 6 and lOw. Compared with the control group, the vessel wall thickness in experimental group was decreased significantly ( P ＜ 0. 01 ) . Conclusions ePTFE cuff can retard the arterialization process of implanted vein.

为了研究出现典型临床症状犬瘟热病犬肝功能的变化,为犬瘟热的治疗及预后提供理论指导,试验采用半自动生化分析仪测定了5例犬瘟热病犬丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)及血清总蛋白(TP)、白蛋白(ALB)、球蛋白(SP)、总胆红素(T-Bil)、直接胆红素(D-Bil)等指标.结果表明:出现典型临床症状犬瘟热病犬的ALT、AST普遍偏高,测定值分别为(84.20±17.68) U/L、(79.20±14.14) U/L;TP、ALB普遍偏低,分别为(53.00±7.31) g/L、(24.80±5.17) g/L;T-Bil、D-Bil、SP浓度分别为(5.96±1.47)μmol/L、(3.88±1.84) μmol/L 和(28.30±8.26) g/L,变化不明显.

选取10条雄性健康犬,随机分成对照组和治疗组,每组5条.实验动物模型采用同侧自体颈静脉行颈动脉45.搭桥.术后均采取常规治疗,治疗组给予肝素钠.分别于术前及术后1、2、3天在颈静脉采血,检测D-二聚体、血清纤维蛋白(原)降解产物(FDP)、血浆血小板a颗粒膜蛋白(GMP-140)、纤溶酶原激活剂的抑制物-1(PAI-1)和组织纤溶酶原激活剂(t-PA)等纤维蛋白溶解系统(纤溶)指标的变化.结果:治疗组与对照组相比,D-二聚体.GMP-140、PAI-1显著降低(P＜0.05),t-PA显著升高(P＜0.05),FDP变化不显著(P＞0.05).结论:肝素钠可改善血管搭桥术后纤溶系统,纠正血液高凝状态,恢复抗凝系统活性.