Objective To examine the expression of Krüppel-like factor-6 (KLF6) in human hepatocellular carcinoma (HCC) and adjacent non-cancerous tissues (ANCT),and further explore the effects and molecular mechanisms of KLF6 on proliferation and invasion of HCC cells.Methods The expression of KLF6 in 50 cases of HCC and corresponding ANCT was detected by immunohistochemical assay.In vitro human HCC SMCC-7721 cells were stably transfected with lentivirus-mediated KLF6 overexpression vector,and the expression levels of proliferating cell nuclear antigen (PCNA) and metalloproteinase-9 (MMP-9) were detected by Western blotting assay.The proliferative activity and invasive capability of HCC cells were detected by MTT and Transwell assays.Results The expression level of KLF6 protein was significantly downregulated in 50％ (25/50) of HCC tissues compared with than in 72％ (36/50) of ANCT.Western blotting results showed that the expression of PCNA and MMP-9 protein in KLF6 group were reduced by 81.5％ and 58.2％,respectively (P ＜ 0.01);MTT assay showed that cell proliferation activity significantly decreased in KLF6 group (P ＜ 0.01);Transwell assay results showed that invasion and metastasis cell number in KLF6 group was significantly reduced [(58.33 ±6.45) cells vs.(98.50±11.33) cells;(58.33 ±6.45) cells vs.(103.25 ±12.17) cells,P＜ 0.01).Conclusion KLF6 is lowly expressed in HCC,and KLF6 overexpression can suppresses HCC cell proliferation and invasion,which mechanism is related to down regulating the expression of PCNA and MMP-9.

胎盘支持下产时胎儿手术是指在保持胎儿胎盘循环的同时进行胎儿先天性缺陷矫治的一种产时胎儿手术[1]。这种手术方案的提出最早是为解决宫内胎儿气道梗阻,随着产前诊断技术的提高,手术适应证范围逐渐扩大,包括先天性膈疝、脐膨出、胎儿颈部肿块、肺或纵隔肿瘤等先天性疾病的治疗[2]。先天性巨型脐膨出是一种新生儿期罕见的严重先天畸形,产时手术治疗

Objective To study the effect of three kinds of warming interventions(thin quilt,heating blanket inflatable and comprehensive warming) for patients' temperature changes during radical gastrectomy. Method 120 patients with radical gastrectomy were randomly divided into three groups: 40 patients in group with thin quilt,40 patients in group with heating blanket inflatable and 40 patients in group with two warming intervention(heating blanket inflatable and fluid warmers.The core temperatures were recorded after entering the operating room,after intubation,during incision,difference time during operation(30,60,90,120,150,and 180 minutes,end of operation) respectively.Result The change trend of three groups of patients' core temperature were significant differences(P<0.05).The core temperatures at time pointes of intubation,incision and difference time during operation were significant difference between thin quilt group and heating blanket inflatable,thin quilt group and comprehensive warming group(P<0.05).there were significant difference in core temperature at difference operation time 60 minutes later after operation among group.Conclusion Thin quilt is less effective for thermal protection.Heating blanket inflatable can prevent perio-perative hypothermia,but it's unstable for temperature maintaining.Comprehensive warming can effectively maintain stable temperature and prevent perioperative hypothermia.

膜瘤占颅内肿瘤的第二位,而矢旁脑膜瘤占颅内脑膜瘤的50%之多[1].脑膜瘤血供丰富,矢旁脑膜瘤以颈外动脉系统供血为主,且常因肿瘤侵犯颅骨使矢状窦处颅骨增厚,开颅过程中出血较多.我院2001年7月～2007年9月手术治疗矢旁脑膜瘤392例,应用高速气钻开颅48例,效果满意.

Objective To discuss the influence of chronic hypoperfusion on the basal forebrain cholinergic system and cognitive function of rats.Methods The chronic hypoperfusion model rats were made by permanent occlusion of bilateral common carotid arteries. The learning and memory performance of rats were examined using Y type maze task. while the basal forebrain changes in pathology observed by microscope and the level of choline acetyltransferase estimated by immunohistochemistry after permanent occlusion 1.2 and 4 months.Results The learning ability of hypoperfusion group was severely impaired and ChAT positive cells and fibers decreased significantly comparing with sham-group(P0.01).while there were a severe loss and necrosis of neuron and glial in basal forebrain during the process. Moreover. as time went on.the learning and memory performance remarkably correlated with the density of cholinergic systems of basal forebrain in hyperperfusion group.Conclusion Cerebral chronic hypoper-fusion induced by permanent occlusion of bilateral common carotid arteries can cause impairment of cholinergic neurons and decline of learning and memory performance of rats.So acetylcholine may play more important role in the process of cognitive impairment. The further pathogenesis remains to be studied.

整体护理在手术室的运行中,局麻下清醒病人手术的整体护理尤为重要.通过对46例局麻下颈椎后入路手术病人实施的整体护理,术前心理护理和术中所需物品的准备、术中的紧密配合和护理、术后的访视和评估消除了病人对手术、麻醉、预后的忧虑及紧张恐惧感,提高了病人对疼痛的耐受力,减轻了病人的痛苦,保障了手术的成功,增进了手术护士对各类知识和技能的掌握和运用,促进了服务质量的提高.本文详细介绍了其具体做法及产生的良好效果.

肝脏血供丰富,暴露困难.在常温下肝门阻断切肝,有严格的时间限制.因此要求护士熟悉肝脏周围的解剖关系,了解手术过程,掌握配合规律,熟悉各种器械的用途和性能,提高配合质量,缩短肝门阻断时间,以减少手术并发症.作者总结了120例肝癌切除的病例,现将手术配合要点和护理体会介绍如下.