Oral diseases, characterized by a high incidence rate and significant impact on patients' quality of life, have emerged as a pressing global health concern amidst escalating societal pressures. Advancements in advanced materials are crucial for the prevention, detection, and treatment of most oral diseases due to their heavy reliance on materials. Nanotechnology has facilitated the development of various nanomaterial-based preparations that have garnered considerable attention in stomatology due to their inherent advantages such as enhanced biosafety, multifunctionality, and targeted therapy capabilities. Responsive nanomaterials offer a breakthrough solution to overcome limitations associated with drug release kinetics, leading to reduced effective drug concentrations while simultaneously exhibiting antibacterial, anti-inflammatory, antioxidant, osteogenic properties along with remineralization functions within safe parameters. Substantial progress has been made towards the long-term prevention and treatment of oral diseases through these responsive nanomaterials. This review begins by elucidating the response mechanisms underlying nanomaterials, followed by an overview of their applications in treating oral diseases. Finally, we discuss the challenges faced and future directions for utilizing advanced materials in addressing treatments for oral diseases. The insights provided herein hold significant implications for both basic research endeavors and clinical translation within stomatology.

Elevated oxidative stress and inflammation, bacterial infections, and vascular impairment undoubtedly impede the normal diabetic wound healing process, which has encouraged the development of high-performance dressings for wound management. Herein, a new type of multiple-crosslinked injectable hydrogel, GCP, was developed via the radical polymerization of propenyl groups and the formation of copper‒polyphenol coordination bonds and Schiff base bonds. The copper‒polyphenol coordination and Schiff base bonds in the GCP hydrogel were disrupted in the acidic microenvironment of diabetic wound, resulting in the release of copper ions and protocatechualdehyde (PA) to scavenge reactive oxygen species (ROS), promote angiogenesis and cell migration, and exert antibacterial and anti-inflammatory activities via the CuPA complexes. Consequently, markedly accelerated infected diabetic wounds healing was achieved through this tissue microenvironment remodeling strategy. Moreover, the underlying mechanism of the antibacterial properties was investigated by 16S rRNA sequencing. The results indicated that the CuPA complexes can clearly inhibit the growth and reproduction of S. aureus by downregulating specific genes associated with ABC transporters, hindering bacterial protein synthesis, and enhancing oxidoreductase activity. This innovative hydrogel platform for wound management may inspire new methods for the preparation of high-performance biomedical materials and the treatment of other clinical diseases.

Post-surgical recurrence and extensive bone defects pose significant challenges during osteosarcoma treatment. These issues can be addressed using a novel strategy that promotes bone repair after removing residual tumors. Therefore, a 3D-printed porous polylactic acid (PLA) scaffold (PH-GBS@CCP) filled with hydrogel and surface-modified with nano-hydroxyapatite (nHA) is designed. The hydrogel, composed of gelatin modified with methacrylic anhydride (GelMA), sodium alginate (SA), and borax, contains Cu-Cys-PEG nanoparticles (CCP) modified with cRGDfk-PEG2K-DSPE. It is injected into the PLA scaffold and crosslinked under UV. This hydrogel acts as a buffer medium between scaffold and bone, reducing cell abrasion, and as a carrier for the responsive release of tumor-targeting CCP. The scaffold provides the support and microenvironment required for bone repair. In early treatment, the acidic tumor microenvironment promotes hydrogel disintegration and CCP release, depleting glutathione and converting Cu2+ to Cu+ for the Fenton-like reaction. This generates reactive oxygen species, strengthening the proptosis effect, and killing the tumor. In later treatment, after tumor elimination, normalized pH and slow CCP release, along with scaffold nHA, promote osteogenic differentiation, providing a sustained osteogenic effect. Overall, the multifunctional composite scaffold achieved the sequential management of post-surgical osteosarcoma through early tumor-killing and later osteogenic effects.

Low immunogenicity, insufficient immune infiltrates, and inhibitive microenvironment of tumors ruthlessly weakened the therapeutic efficacy of immunotherapies. How to improve the therapy efficacy by reshaping the tumor immune microenvironment still remains a challenge that needs to be urgently addressed. Here, a novel dual-defect nitrogen-rich carbon nitride-based heterostructural nanocatalyst (CNO@CuMS) possessing brilliant piezocatalytic H2O2 generation, sonocatalytic 1O2 generation, Fenton-like catalytic •OH generation, and cell cuproptosis inducing properties are developed. Accompanied by the ultrasonic treatment, immunogenic cell death (ICD) and the subsequent releasing of damage-associated molecular patterns (DAMPs) of tumor cells are successfully triggered by CNO@CuMS, which resulted in the activation of systematic antitumor response and enhanced the infiltration of CD8+ cytotoxic T lymphocytes and natural killer (NK) cells at tumor site. The large quantitatively generated reactive oxygen species (ROS) also promoted the polarization of M2-type macrophage to M1-type as well. Benefitting from this tumor immune microenvironment remodelling effect, remarkable malignant orthotopic osteosarcoma therapy efficacy is achieved employing CNO@CuMS + αPD-1 combination strategy under ultrasonic treatment, with no significant side effects are observed. This research not only proposes a method to fabricate high proformance carbon nitride-based nanocatalyst but also provides a new strategy to realize highly efficient malignant bone tumor therapy during clinical practice.

Photoacoustic (PA) imaging is a burgeoning imaging modality that has a broad range of applications in the early diagnosis of cancer, detection of various diseases, and relevant scientific research. It is a non-invasive imaging modality that relies on the absorption coefficient of the imaging tissue and the injected PA-imaging contrast agent. Nevertheless, PA imaging exhibits weak imaging depth due to its exponentially decaying signal intensity with increasing tissue depth. To improve the depth and heighten the contrast of imaging, a series of PA contrast agents has been developed based on nanomaterials. In this review, we present a comprehensive overview of recent advancements in contrast agents for photoacoustic (PA) imaging, encompassing the emergence of first near-infrared region (NIR-I, 700-950 nm) PA contrast agents, second near-infrared region (NIR-II, 1000-1700 nm) PA contrast agents, and ratiometric PA contrast agents. Subsequently, the latest advances in PA image-guided cancer therapy were introduced, such as photothermal therapy (PTT), photodynamic therapy (PDT), sonodynamic therapy (SDT), and PTT-based synergistic therapy. Finally, the prospects of PA contrast agents and their biomedical applications were also discussed. This review provides a systematic summary of the development and utilization of the cutting-edge photoacoustic agents, which may inspire fresh thinking in the fabrication and application aspects of imaging agents.

To build a smart system in response to the variable microenvironment in infected diabetic wounds, a multifunctional wound dressing was constructed by co-incorporating glucose oxidase (GOx) and a pH-responsive selfassembly Cu2_ xSe-BSA nanozyme into a dual-dynamic bond cross-linked hydrogel (OBG). This composite hydrogel (OBG@CG) can adhere to the wound site and respond to the acidic inflammatory environment, initiating the GOx-catalyzed generation of H2O2 and the self-assembly activated peroxidase-like property of Cu2_xSeBSA nanozymes, resulting in significant hydroxyl radical production to attack the biofilm during the acute infection period and alleviate the high-glucose microenvironment for better wound healing. During the wound recovery phase, Cu2_ xSe-BSA aggregates disassembled owing to the elevated pH, terminating catalytic reactive oxygen species generation. Simultaneously, Cu2+ released from the Cu2_xSe-BSA not only promotes the production of mature collagen but also enhances the migration and proliferation of endothelial cells. RNA-seq analysis demonstrated that OBG@CG exerted its antibacterial property by damaging the integrity of the biofilm by inducing radicals and interfering with the energy supply, along with destroying the defense system by disturbing thiol metabolism and reducing transporter activities. This work proposes an innovative glucose consumption strategy for infected diabetic wound management, which may inspire new ideas in the exploration of smart wound dressing.

Chronic inflammatory diseases, such as intervertebral disc degeneration (IVDD), which affect the lives of hundreds of millions of people, still lack effective and precise treatments. In this study, a novel hydrogel system with many extraordinary properties is developed for gene–cell combination therapy of IVDD. Phenylboronic acid‐modified G5 PAMAM (G5‐PBA) is first synthesized, and therapeutic siRNA silencing the expression of P65 mixed with G5‐PBA (siRNA@G5‐PBA) is then embedded into the hydrogel (siRNA@G5‐PBA@Gel) based on multi‐dynamic bonds including acyl hydrazone bonds, imine linkage, π – π stacking, and hydrogen bonding interactions. Local and acidic inflammatory microenvironment‐responsive gene‐drug release can achieve spatiotemporal regulation of gene expression. In addition, gene‐drug release from the hydrogel can be sustained for more than 28 days in vitro and in vivo, greatly inhibiting the secretion of inflammatory factors and the subsequent degeneration of nucleus pulposus (NP) cells induced by lipopolysaccharide (LPS). Through prolonged inhibition of the P65/NLRP3 signaling pathway, the siRNA@G5‐PBA@Gel is verified to relieve inflammatory storms, which can significantly enhance the regeneration of IVD when combined with cell therapy. Overall, this study proposes an innovative system for gene–cell combination therapy and a precise and minimally invasive treatment method for IVD regeneration.

Catalytic tumor therapy based on two-dimensional (2D) nanomaterials is a burgeoning and promising tumor therapeutic modality. However, the inefficient utilization and conversion of exogenous stimulation, single catalytic modality, and unsatisfactory therapeutic efficiency in the tumor microenvironment (TME) have seriously restricted their further application in tumor therapy. Herein, the heterogeneous carbon nitride-based nanoagent named T-HCN@CuMS was successfully developed, which dramatically improved the efficiency of the tumor therapeutic modality. Benefiting from the donor-acceptor (triazine-heptazine) structure within the heterogeneous carbon nitride nanosheets (HCN) and the construction of interplanar heterostructure with copper loaded metallic molybdenum bisulfide nanosheets (CuMS), T-HCN@CuMS presented a favorable photo-induced catalytic property to generate abundant reactive oxygen species (ROS) under near-infrared (NIR) light irradiation. Besides, the choice of CuMS simultaneously enabled this nanoagent to efficiently catalyze the Fenton-like reaction and trigger cell cuproptosis, a recently recognized regulated cell death mode characterized by imbalanced intracellular copper homeostasis and aggregation of lipoylated mitochondrial proteins. Moreover, upon surface modification with cRGDfk-PEG2k-DSPE, T-HCN@CuMS was prepared and endowed with improved dispersibility and alpha(v)beta(3) integrins targeting ability. In general, through the rational design, T-HCN@CuMS was facilely prepared and had achieved satisfactory antitumor and antimetastasis outcomes both in vitro and in a high-metastatic orthotopic osteosarcoma model. This strategy could offer an idea to treat malignant diseases based on 2D nanomaterials.

Continuous bleeding, bacterial infection, oxidative stress, and vascular damage within a diabetic wound microenvironment necessitate the development of multifunctional dressings that match the complex physio-logical process of skin healing. Herein, copper-tannic acid (CuTA) nanosheets were first synthesized by chelating copper ions with tannic acid (TA). These nanosheets were subsequently integrated into an injectable self-healing hydrogel comprising methacrylic anhydride-modified gelatin (GelMA), cationic guar gum (CG), and borax. Through free-radical polymerization as well as hydrogen and borate ester bonds formation, we fabricated an acidic and highly reactive oxygen species (ROS)-responsive composite hydrogel named GGB-CT, which exhibited remarkable hemostasis and adhesion. With responsive decomposition of GGB-CT, the released CuTA efficiently scavenged excess ROS as well as effectively killed bacteria by inhibiting arginine synthesis, blocking the tricarboxylic acid cycle and inducing cuproptosis-like death in the tested microbes. In the later stages of wound healing, the composite hydrogel substantially promoted macrophage polarization and angiogenesis, thus accelerating the re-epithelialization of the wound area. Overall, this study proposes an innovative hydrogel for treating infected diabetic wounds and may inspire new thinking of high-performance hydrogels for biomedical applications.

We report a colorimetric method for glucose detection based on Au nanoparticle-decorated WSe2 (Au@WSe2) hybrid nanostructures. These hybrid structures are easily synthesized by simply stirring HAuCl4 precursor with WSe2 nanosheets in aqueous solution. Owing to strong synergistic catalytic effects of Au nanoparticles and WSe2 nanosheets, the Au@WSe2 hybrid nanostructures exhibit enhanced peroxidase-like activity (about 2-fold higher compared to WSe2 nanosheets alone) for 3,3’,5,5’-tetramethylbenzidine oxidation by H2O2. Based on the highly catalytical property, the colorimetric method for glucose detection is established by coupling glucose oxidase (GOx). The detection limit of glucose is 3.66 µM. Moreover, the proposed colorimetric method is applicable to glucose detection in serum samples and is promising for applications in biomedical fields.

Background Research on clinical trials that employ stem cells to treat children's diseases is limited. The clinical trial registry database provides a unique window to us to get known about clinical trial researches with different statuses. However, few studies aimed to perform a comprehensive and thorough analysis of those registered trials in the aforementioned field based on ClinicalTrials.gov and the ICTRP portal site. Methods Our study covered the clinical researches about stem cell therapy enrolling subjects aged under 18 years old registered on ClinicalTrials.gov and WHO ICTRP before May 18, 2021. A cross-sectional study was implemented to comprehensively describe and analyze the included trials that met the criteria. Results were available on ClinicalTrials.gov, and publications related to the included trials were identified. All analyses were performed utilizing the SPSS 25.0 software. Results Eventually, 202 clinical trials were included and evaluated. The participant number of trials tended to be small; 71.3% were enrolled < 50. And 93.5% of the subjects were without gender restrictions. Till May 2020, 112 trials had been preliminary completed, of which only 39 trials had published papers or uploaded results. Most (73.6%) of 186 interventional trials were in phase 1 and phase 2, where 131 (70.4%) trials were conducted without masking, and 26.3% trials were randomized; 55.4% trials were performed single group assignment. Of 16 observational trials, case-only/series took up 37.5%. Hematopoietic stem cells (37.1%) and mesenchymal stem cells (36.1%) were mostly employed, while umbilical cord blood (UCB)-derived cells (24.3%) and bone marrow (BM)-derived cells (20.8%) were the major sources. Conclusions This study provided an overall picture of utilizing stem cells for treatment and management of childhood diseases. Since clinical trials in this area are insufficient in quantity and quality, there is an urgent need of larger, better-designed trials. Increased investment in clinical research of stem cell treatment products should be carried out to achieve the transformation of results as soon as possible. Moreover, it is important to optimize the management of the registration platform and shorten the time it takes for research results to be published.

Engineering a proper immune response following biomaterial implantation is essential to bone tissue regeneration. Herein, a biomimetically hierarchical scaffold composed of deferoxamine@poly(ε-caprolactone) nanoparticles (DFO@PCL NPs), manganese carbonyl (MnCO) nanosheets, gelatin methacryloyl hydrogel, and a polylactide/hydroxyapatite (HA) matrix is fabricated to augment bone repair by facilitating the balance of the immune system and bone metabolism. First, a 3D printed stiff scaffold with a well-organized gradient structure mimics the cortical and cancellous bone tissues; meanwhile, an inside infusion of a soft hydrogel further endows the scaffold with characteristics of the extracellular matrix. A Fenton-like reaction between MnCO and endogenous hydrogen peroxide generated at the implant-tissue site triggers continuous release of carbon monoxide and Mn2+ , thus significantly lessening inflammatory response by upregulating the M2 phenotype of macrophages, which also secretes vascular endothelial growth factor to induce vascular formation. Through activating the hypoxia-inducible factor-1α pathway, Mn2+ and DFO@PCL NP further promote angiogenesis. Moreover, DFO inhibits osteoclast differentiation and synergistically collaborates with the osteoinductive activity of HA. Based on amounts of data in vitro and in vivo, strong immunomodulatory, intensive angiogenic, weak osteoclastogenic, and superior osteogenic abilities of such an osteoimmunity-regulating scaffold present a profound effect on improving bone regeneration, which puts forward a worthy base and positive enlightenment for large-scale bone defect repair.

Reactive oxygen species (ROS) played an essential role in regulating various physiological functions in living organisms and treatment of malignant disease. Herein, a new type of PEGylated carbon nitride nanosheet@copper-doped polyaniline (CNNS@CuPANI) with nano-heterostructure and dual-modal catalytic ROS generation performance was synthesized. Formation of the nano-heterostructure promoted separation of photo-induced electron-hole pairs, and doping of Cu2+ further increased electron-transfer rate greatly. Besides, antenna effect of the CuPANI endowed the heterostructure with near-infrared light utilization ability, finally inducing concurrences of oxygen-independent type I and oxygen-dependent type II photodynamic therapy simultaneously. Interestingly, unleashing of doped Cu2+ ions responded to reductive glutathione in tumor microenvironment further triggered a Fenton-like reaction, and such PEGylated CNNS@CuPANI with remarkable ROS generation ability was therefore employed for high-metastatic orthotopic osteosarcoma therapy. Cell proteomics analysis illustrated that cell survival and adhesion were considerably altered by the massively-generated ROS, which also affected efficiencies of conspicuous orthotopic tumor therapy and pulmonary metastasis resistance in vivo. To summarize, our newly-proposed PEGylated CNNS@CuPANI achieved desirable effects on killing orthotopic osteosarcoma and preventing metastasis, providing a new therapeutic strategy for malignant tumor treatment.

Inflammatory responses of nucleus pulposus (NP) can induce imbalanced anabolism and catabolism of extracellular matrix, and the cytosolic dsDNA accumulation and STING-NF-κB pathway activation found in NP inflammation are considered as fairly important cause of intervertebral disc (IVD) degeneration. Herein, we constructed a siSTING delivery hydrogel of aldehyde hyaluronic acid (HA-CHO) and poly(amidoamine) PAMAM/siRNA complex to intervene the abnormal STING signal for IVD degeneration treatment, where the formation of dynamic Schiff base bonds in the system (siSTING@HPgel) was able to overcome the shortcomings such as low cellular uptake, short half-life, and rapid degradation of siRNA-based strategy. PAMAM not only formed complexes with siRNA to promote siRNA transfection, but also served as dynamic crosslinker to construct hydrogel, and the injectable and self-healing hydrogel efficiently and steadily silenced STING expression in NP cells. Finally, the siSTING@HPgel significantly eased IVD inflammation and slowed IVD degeneration by prolonging STING knockdown in puncture-induced IVD degeneration rat model, revealing that STING pathway was a therapeutic target for IVD degeneration and such novel hydrogel had great potential for being applied to many other diseases for gene delivery.

Objective:To correlate homocysteine (Hcy) and blood lipid levels with neurological function in patients with progressive ischemic stroke.Methods:A total of 400 patients with ischemic stroke who received treatment between June 2018 and June 2020 in Linhai Second People's Hospital were included in this study. Progressive ischemic stroke ( n = 126) and non-progressive ischemic stroke ( n = 274) groups were designated. Hcy level was determined by enzyme-linked immunosorbent assay. High-density lipoprotein cholesterol, triacylglycerol, low-density lipoprotein cholesterol and cholesterol levels were measured using a biochemical analyzer. Hcy and blood lipid levels as well as National Institute Health of Stroke Scale (NIHSS) score were determined in each group. Hcy and blood lipid levels were correlated with NIHSS score. Results:Hcy level in the progressive ischemic stroke group was significantly higher than that in the non-progressive ischemic stroke group [(28.39 ± 4.36) μmol/L vs. (20.17 ± 3.24) μmol/L, t = 18.894, P < 0.05]. Low-density lipoprotein cholesterol , triacylglycerol and TC levels in the progressive ischemic stroke group were (3.29 ± 0.45) mmol/L, (2.08 ± 0.34) mmol/L and (4.82 ± 0.79) mmol/L, respectively, which were significantly higher than those in the non-progressive ischemic stroke group [(2.48 ± 0.37) mmol/L, (1.56 ± 0.29) mmol/L and (4.08 ± 0.43) mmol/L, t = 17.644, 14.859, 9.860, P < 0.05]. High-density lipoprotein cholesterol level in the progressive ischemic stroke group was significantly lower than that in the non-progressive ischemic stroke group [(1.03 ± 0.13) mmol/L vs. (1.19 ± 0.14) mmol/L, t =11.158, P < 0.05]. NIHSS score in the progressive ischemic stroke group was significantly higher than that in the non-progressive ischemic stroke group [(21.72 ± 4.35) points vs. (15.52 ± 2.89) points, t = 14.582, P < 0.05]. Hcy, low-density lipoprotein cholesterol, cholesterol and triacylglycerol levels were linearly and positively correlated with NIHSS score ( r = 0.846, 0.724, 0.718, 0.765, all P < 0.05), while igh-density lipoprotein cholesterol level was linearly and negatively correlated with NIHSS score ( r = -0.710, P < 0.05). Conclusion:In patients with progressive ischemic stroke, Hcy level is increased and blood lipid level is obviously abnormal. Hcy and blood lipid levels are greatly correlated with neurological function.

Clinically approved photodynamic therapy (PDT) has emerged as an alternative treatment for cancers and malignant diseases; however, high quality PDT agents were still in great demand. Herein, the van der Waals (vdW) heterostructure of graphitic carbon nitride (g-C3N4, abbreviated as CN here) and metallic molybdenum disulfide (1T-MoS2, abbreviated as MS here) was fabricated, PEGylated, and then investigated. By making use of the extraordinary antenna effect and the ultrafast electron transfer rate of metallic molybdenum disulfide, as well as the efficient separation of photogenerated electron-hole pairs of the final heterojunction, massive reactive oxygen species (ROS) can be generated under 670 nm laser irradiation together with the as-synthesized g-C3N4@1T-MoS2 vdW nanostructure (CNMS), which should be largely owed to the appealing photocatalytic water splitting property of the CNMS structure. It was well proved by the in vitro studies that the intracellularly produced ROS can result in cell death, by the way of inducing cell apoptosis and/or necrosis through mediating phosphatidylserine ectropion, mitochondrial depolarization, and chromosomal DNA fragmentation, as well as up-regulating the expression of apoptosis-related proteins and unbalancing intracellular redox homeostasis. In vivo exploration also gave out satisfactory results that the tumor growth could be significantly inhibited by the photodynamic therapy. Additionally, outstanding biocompatibility and biosafety were also guaranteed. All these results provided compelling evidences for that the CNMS vdW heterostructure is an aussichtsreich nano-photosensitizer, and may provide some fresh ideas for the developing of nanomedicine for PDT application.

• An H 2 O 2 induced oxidative stress model of the endplate was successfully established in vitro as well as in vivo. • NAC alleviated cellular damage, abrogated catabolic effects and restored the redox status and cell viability in endplates . • The inhibitor of TAK1 reduced the expression of p-TAK1 and NLRP3, and ameliorated rat CEP degeneration in vivo.

We developed dual biologically responsive nanogapped gold nanoparticle vesicles loaded with immune inhibitor and carrying an anticancer polymeric prodrug for synergistic concurrent chemo-immunotherapy against primary and metastatic tumors, along with guided cargo release by photoacoustic (PA) imaging in the second near-infrared (NIR-II) window. The responsive vesicle was prepared by self-assembly of nanogapped gold nanoparticles (AuNNPs) grafted with poly(ethylene glycol) (PEG) and dual pH/GSH-responsive polyprodug poly(SN38-co-4-vinylpyridine) (termed AuNNP@PEG/PSN38VP), showing intense PA signal in the NIR-II window. The effect of the rigidity of hydrophobic polymer PSN38VP on the assembled structures and the formation mechanism of AuNNP@SN38 Ve were elucidated by computational simulations. The immune inhibitor BLZ-945 was encapsulated into the vesicles, resulting in pH-responsive release of BLZ-945 for targeted immunotherapy, followed by the dissociation of the vesicles into single AuNNP@PEG/PSN38VP. The hydrophilic AuNNP@PEG/PSN38VP nanoparticles could penetrate deep into the tumor tissues and release the anticancer drug SN38 under the reductive environment. A PA signal in the NIR-II window in the deep tumor region was obtained. The BLZ-945-loaded vesicle enabled enhanced PA imaging-guided concurrent chemo-immunotherapy efficacy, inhibiting the growth of both primary tumors and metastatic tumors.

The establishment of analytical methods with superior sensitivity, selectivity, accuracy, and stability, as well as ultralow detection limits inspired a broad range of research enthusiasm. In this work, we developed an electrochemical method for detecting mercury ions using metallic 1T-MoS2 nanosheets, which was firstly fabricated using phase engineering strategy and then exfoliated. Remarkably promoted sensitivity and significantly lowered limits of detection (LODs) were achieved due to the dramatically reduced charge transfer resistance of 1T-MoS2 nanosheets compared with that of the semiconducting 2H-MoS2 nanoflakes. After electrode modification and optimization, working curves for both two materials were obtained. Astonishingly, the LODs of 1T-MoS2 modified glassy carbon electrodes (GCEs) was calculated to be 1.54 x 10(-19) M (D-L = 3 sigma/k), which was six orders of magnitude lower than that of 2H-MoS2 (1.46 x 10(-13) M). And 1T-MoS2-based electrochemical detection method also exhibited superior selectivity towards other ten ions selected because of the high affinity between Hg and S. The stability and reproducibility of the new method was impressive as well, and the recovery rates using spiked real water samples were all in the range of 99.8-101.2% due to the superb sensitivity. All results proved that the metallic 1T-MoS2 nanosheets a promising material for electrochemical detection of mercury ions. (c) 2020 Elsevier Ltd. All rights reserved.