PDF file - 107K, Supplemental Tables 1A and 1B: Agreement between worst study histopathologic diagnosis. Supplemental Table 2: The relationship of E6 and DNA detection of human papillomavirus (HPV) genotypes 16, 18, and/or 45 (HPV16/18/45) on cervical exfoliated cells with HPV genotypes detected in the tissue of cervical intraepithelial neoplasia grade 2 (CIN2), grade 3 (CIN3), and cervical cancer, overall (all) and by clinical site.

This paper summarizes the concept of self-aging perception, evaluation scales, and the impact of self-aging perception on elderly cancer patients'' mortality, supporting factors, lifestyle, medical compliance, emotional and mental status, physiological function, cognitive function, in order to provide reference for the related research on self-aging perception of elderly cancer patients.

Background Although colonoscopy is the standard screening test for colorectal cancer (CRC), its use is limited by a poor compliance rate, the need for extensive bowel preparation, and the risk of complications. As an alternative, an FDA-approved stool-based DNA test, Cologuard, has demonstrated satisfactory detection performance for CRC, but its compliance rate remains suboptimal, primarily attributable to individuals’ reluctance to provide stool samples. Methods We developed a noninvasive blood-based CRC test, ColonSecure, based on cell-free DNA containing cancer-specific CpG island methylation patterns. We initially screened publicly available datasets for differentially methylated CpG sites in CRC with prediction potential. Subsequently, we performed two sequential bisulfite-free methylation sequencing on blood samples obtained from CRC patients and non-cancer controls. Through rigorous evaluation of each marker and machine learning-assisted feature selection, we identified 149 hypermethylated markers from over 193,000 CpG sites. These markers were then utilized to construct the ColonSecure model, enabling accurate CRC detection. Results We validated the efficacy of our cell-free DNA methylation-based blood test for CRC screening with 3493 high-risk individuals identified from 114,136 urban residents. The ColonSecure test identified 89 out of 103 CRC patients diagnosed by the follow-up colonoscopy, outperforming CEA, CRP, and CA19-9 (with a sensitivity of 86.4% compared to 45.6%, 39.8%, and 25.2% for CEA, CRP, and CA19-9 respectively; an AUROC of 0.956 compared to an AUROC of < 0.77 for other methods). Conclusion Our observations emphasize the potential of our multiple cfDNA methylation marker-based test for CRC screening in high-risk populations.

目的:构建肿瘤手术病人外周静脉输液性静脉炎发生风险预测模型.方法:选取2021年4月—2022年9月在我院手术室行外科手术的肿瘤住院病人1178例作为研究对象,采用前瞻性研究设计,收集病人一般资料及手术相关资料并随访至病人术后72 h.以美国静脉输液护理学会的输液性静脉炎分级标准作为评价标准,采用单因素分析及Logistic回归分析探讨病人外周静脉发生输液性静脉炎的影响因素,并建立回归方程.结果:1178例病人中67例发生输液性静脉炎,发生率为5.69％,术后6 h～24 h发生率最高.Logistic回归分析显示,肿瘤病人吸烟史、穿刺部位、穿刺肢体、一次穿刺、输液加温进入回归方程,并构建病人外周静脉输液性静脉炎风险预测模型,模型校准度良好.结论:构建的肿瘤手术病人外周静脉输液性静脉炎发生风险预测模型具有良好的预测性能,可以为临床早期识别肿瘤手术病人输液性静脉炎发生风险提供参考.

Background This study aimed to analyze the clinical and pathological features of extrauterine endometrial stromal sarcoma (EESS) and explore an effective therapeutic regimen to reduce the recurrence rate in low-grade EESS patients. Methods Ten LG-EESS patients who were treated at the Chinese Academy of Medical Sciences Cancer Institute and Hospital from June 1999 to June 2019 were collected and analyzed. Results (1) Patient demographics are summarized in manuscript. Preoperative CA125 examination showed that 8 patients had a median level of 49.5 U/L (15.4–168.0 U/L). (2) All ten patients underwent tumor cytoreductive surgery. Five patients underwent optimal tumor resection and achieved an R0 resection. After the initial surgery, 7 patients who had multiple metastasis were treated with adjuvant chemotherapy, 2 patients with vaginal ESS were treated with chemotherapy and radiation therapy, and 6 patients with ER/PR positive received hormone therapy with or without chemotherapy. (2) Most EESS patients had multiple tumors. The omentum was the most commonly affected site, followed by the ovaries. (3) The median follow-up was 94 (range: 27–228) months, and recurrence was observed in 3 patients ( n = 10, 30%) who underwent non-optimal surgery and no hormone therapy. The 5-year and 10-year DFS rates were both 70%, as shown in Fig. 2. OS was both 100% at 5 and 10 years. Conclusion As a conclusion, EESS is a rare disease and LG-EESS has a good prognosis. Surgery remains the available treatment for patients. LG-EESS has a risk of late recurrence which requires a long-term follow-up. With a limited sample size, our study shows optimal tumor reductive surgery and adjuvant hormone therapy may significantly reduce the risk of recurrence.

目的 探讨预见性护理对胸腔镜肺叶切除患者全身麻醉(简称全麻)苏醒期疼痛、躁动及躁动相关不良事件发生情况的影响.方法 选择该院2020年9月至2021年3月行胸腔镜肺叶切除术的患者90例,采用区组随机化方法产生随机编码,将其分为对照组和观察组,每组45例.对照组采用常规护理,观察组采用预见性护理.比较两组患者全麻苏醒期疼痛、躁动发生情况及躁动相关不良事件的发生率、患者满意度的差异.结果 观察组患者全麻苏醒期的疼痛评分低于对照组(P<0.05),苏醒期躁动的发生率与严重程度及躁动相关不良事件的发生率均低于对照组,差异均有统计学意义(P<0.05).观察组的体温高于对照组,差异有统计学意义(P<0.05).结论 预见性护理可改善胸腔镜肺叶切除术患者全麻苏醒期的疼痛、躁动及躁动相关不良事件的发生,并提高医疗护理安全.

Biological and synthetic meshes were used to cover the damaged muscle and augment the subpectoral pocket in breast reconstruction. However, few studies have directly compared the effects of biological and synthetic meshes. This study analyzed postoperative complications and assessed the patient-reported outcomes with the use of BioDesign® Surgisis and TiLOOP Bra/TiMesh® in one-stage implant-based breast reconstruction. Patients undergoing one-stage implant-based breast reconstruction were enrolled in this study. Post-mastectomy breast reconstructions were facilitated with either Surgisis mesh or TiLOOP mesh. Complications were examined and patient-reported quality-of-life outcomes were evaluated using the BREAST-Q questionnaire (ver 2.0). The multivariate linear regression models were used for data analysis. Overall, 79 of 116 patients (68%) received breast reconstruction with Surgisis mesh and 37 (32%) with TiLOOP mesh. There was no difference in complication rates between the two groups postoperatively. But patient-reported satisfaction was higher with the use of Surgisis mesh than with TiLOOP mesh (P = 0.05). This study reported no difference between the Surgisis group and the TiLOOP group in either complication rates or most patient-reported outcomes postoperatively. Yet the assessment of patient-reported satisfaction showed preference toward Surgisis mesh, a finding with a potential implication for mesh selection.

BackgroundImplant-based breast reconstruction is increasingly becoming the most common method of postmastectomy breast reconstruction in use today. As the traditional autologous reconstruction technique, latissimus dorsi flap (LDF) is employed by surgeons for reconstruction after breast cancer surgery, including partial mastectomy, modified radical mastectomy, and others. The authors aim to compare patient-reported outcomes (PROs) and complications between the SIS matrix-assisted direct-to-implant (DTI) breast reconstruction and the autologous LDF breast reconstruction.MethodsPatients undergoing the SIS matrix-assisted DTI reconstruction or mastectomy with LDF reconstruction or partial mastectomy with mini latissimus dorsi flap (MLDF) reconstruction were enrolled in a single institution from August 2010 to April 2019. Patients were included for analysis and divided into three groups: those who underwent LDF reconstruction, those who underwent MLDF reconstruction, and patients who underwent SIS matrix-assisted DTI breast reconstruction. PROs (using the BREAST-Q version 2.0 questionnaire) and complications were evaluated.ResultsA total of 135 patients met the inclusion criteria: 79 patients (58.5%) underwent SIS matrix-assisted DTI, 29 patients (21.5%) underwent LDF breast reconstruction, and 27 patients (20%) underwent MLDF breast reconstruction. PROs and complication rates between LDF reconstruction group and MLDF reconstruction group showed no statistically significant differences. Furthermore, BREAST-Q responses found that patients in the whole autologous LDF reconstruction group had better psychosocial well-being, showing a mean score of 84.31 ± 17.28 compared with SIS matrix-assisted DTI reconstruction, with a mean score of 73.52 ± 19.96 (p = 0.005), and expressed higher sexual well-being (69.65 ± 24.64 vs. 50.95 ± 26.47; p = 0.016). But there were no statistically significant differences between the two groups for postoperative complications.ConclusionThis retrospective study showed no statistically significant differences between LDF breast reconstruction and MLDF breast reconstruction. However, patients in the whole autologous LDF reconstruction group yielded superior PROs than patients in the SIS matrix-assisted DTI reconstruction group in the psychosocial well-being and sexual well-being domains.

BACKGROUND Breast cancer (BC) is the most common malignant tumor in women. AIM To investigate BC-associated hub genes to obtain a better understanding of BC tumorigenesis. METHODS In total, 1203 BC samples were downloaded from The Cancer Genome Atlas database, which included 113 normal samples and 1090 tumor samples. The limma package of R software was used to analyze the differentially expressed genes (DEGs) in tumor tissues compared with normal tissues. The cluster Profiler package was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of upregulated and downregulated genes. Univariate Cox regression was conducted to explore the DEGs with statistical significance. Protein-protein interaction (PPI) network analysis was employed to investigate the hub genes using the CytoHubba plug-in of Cytoscape software. Survival analyses of the hub genes were carried out using the Kaplan-Meier method. The expression level of these hub genes was validated in the Gene Expression Profiling Interactive Analysis database and Human Protein Atlas database. RESULTS A total of 1317 DEGs (fold change > 2; P < 0.01) were confirmed through bioinformatics analysis, which included 744 upregulated and 573 downregulated genes in BC samples. KEGG enrichment analysis indicated that the upregulated genes were mainly enriched in the cytokine-cytokine receptor interaction, cell cycle, and the p53 signaling pathway (P < 0.01); and the downregulated genes were mainly enriched in the cytokine-cytokine receptor interaction, peroxisome proliferator-activated receptor signaling pathway, and AMP-activated protein kinase signaling pathway (P < 0.01). CONCLUSION In view of the results of PPI analysis, which were verified by survival and expression analyses, we conclude that MAD2L1, PLK1, SAA1, CCNB1, SHCBP1, KIF4A, ANLN, and ERCC6L may act as biomarkers for the diagnosis and prognosis in BC patients.

输液性静脉炎在临床上较为常见.输液性静脉炎的发生可增加患者的痛苦,影响其正常治疗.故预防输液性静脉炎的发生十分必要.本文在查阅相关文献的基础上对输液性静脉炎的各类预防措施进行综述,以期为临床上防治输液性静脉炎提供参考.

Although the prognosis of locally advanced cervical cancer has improved dramatically, survival for those with stage IIIB-IVA disease or lymph nodes metastasis remains poor. It is believed that the incorporation of intensity-modulated radiotherapy into the treatment of cervical cancer might yield an improved loco-regional control, whereas more cycles of more potent chemotherapy after the completion of concurrent chemotherapy was associated with a diminished distant metastasis. We therefore initiated a non-randomized prospective phaseII study to evaluate the feasibility of incorporating both these two treatment modality into the treatment of high risk locally advanced cervical cancer. To determine whether the incorporation of intensity-modulated radiotherapy and the addition of adjuvant paclitaxel plus cisplatin regimen into the treatment policy for patients with high risk locally advanced cervical cancer might improve their oncologic outcomes. Patients were enrolled if they had biopsy proven stage IIIA-IVA squamous cervical cancer or stage IIB disease with metastatic regional nodes. Intensity-modulated radiotherapy was delivered with dynamic multi-leaf collimators using 6MV photon beams. Prescription for PTV ranged from 45.0 50.0 Gy at 1.8 Gy 2.0 Gy/fraction in 25 fractions. Enlarged nodes were contoured separately and PTV-nodes were boosted simultaneously to a total dose of 50.0–65 Gy at 2.0- 2.6 Gy/fraction in 25 fractions. A total dose of 28 35 Gy high-dose- rate brachytherapy was prescribed to point A in 4 5 weekly fractions using an iridium- 192 source. Concurrent weekly intravenous cisplatin at 30 mg/m2 was initiated on the first day of radiotherapy for over 1-h during external-beam radiotherapy. Adjuvant chemotherapy was scheduled within 4 weeks after the completion of concurrent chemo-radiotherapy and repeated 3 weeks later. Paclitaxel 150 mg/m2 was given as a 3-h infusion on day1, followed by cisplatin 35 mg/m2 with 1-h infusion on day1-2 (70 mg/m2 in total). Fifty patients achieved complete response 4 weeks after the completion of the treatment protocol, whereas 2 patients had persistent disease. After a median follow-up period of 66 months, loco-regional (including 2 persistent disease), distant, and synchronous treatment failure occurred in 4,5, and 1, respectively. The 5-year disease-free survival, loco-regional recurrence-free survival, distant-metastasis recurrence-free survival was 80.5

Objective:To search the literatures related to surgical isolation technology of malignant tumor in China from 2011 to 2020 and conduct bibliometric analysis.Methods:Based on the literature retrieval system of the Union Medical College, "surgical isolation technique for malignant tumor" and other search terms were used to retrieve literature from CNKI, Wanfang Database, VIP, China Biomedical Literature Database, PubMed, Embase, CINAHL Nursing Database. The retrieval time was from January 1, 2011 to December 31, 2020. NoteExpress software was used for literature management and analysis.Results:A total of 21 034 Chinese literatures and 1 509 English literatures were retrieved. After screening according to the exclusion criteria, 304 literatures were finally included, all of which were Chinese literatures. Among them, there were 119 treatises, 95 nursing coordination articles, 68 experience summary articles, 21 reviews and 1 expert consensus. They were distributed in 125 medical journals. There were 37 core journal papers (12.17%) and 23 foundation papers (7.57%) . Journal of Frontiers of Medicine, Journal of Medical Information and World Latest Medicine Information were the top 3 periodicals with the most publications. The literature came from 261 publishing institutions, and each institution published an average of 1.16 papers. The top 3 provinces in terms of published papers were Jiangsu Province (46 papers) , Sichuan Province (25 papers) and Guangdong Province (20 papers) . The top 3 high-frequency words were "tumor-free technology" "laparoscopy" and "malignant tumor". The co-authorship rate of the literature was 48.68%, the degree of cooperation was 1.91 and the average citation frequency of the literature was 3.57 times. Conclusions:Research on surgical isolation technology for malignant tumors in China is gradually deepening and developing, but there are still some problems, such as low overall research quality, obvious regional differences, lack of depth and systematization and single research content. It is necessary to carry out higher quality research.

Background: Lymph node metastasis (LNM) accounts for the most important route of metastasis for cervical cancer. Yet, the status of LNM is different in patients with similar clinico-pathological variables. It has been revealed that microRNAs are widely involved in the occurrence and development of various malignancies, and the tumor-suppressive or promoting effects of microRM-99 (miR-99) family have been previously reported. This study sought to investigate the predictive value of miR-99a for lymphogenous spread and its effect on the survival of patients with early-stage cervical squamous cell cancer (CSCC). Methods: Patients with stage IB squamous cervical cancer who were treated surgically between October 2015 and November 2018 were enrolled. A total of 21 formalin-fixed paraffin-embedded tissues of pathologically confirmed positive lymph nodes were retrieved, and an additional 21 tissues of negative lymph nodes from patients well-matched on baseline characteristics were collected as the control group. TaqMan real-time quantitative polymerase chain reaction was used to examine the expression levels of miR-99a in the samples. Differential expression levels of miR-99a were compared between the 2 groups using independent sample t-test. Furthermore, the associations between miR-99a expression level and clinico-pathological parameters of these 42 patients was evaluated by Chi-square test or Fisher's exact-probability method, and their effects on survival were assessed using Kaplan-Meier product-limit method. Results: There were no significant differences in baseline clinico-pathological parameters between the 2 groups (P>0.05). The expression levels of miR-99a in the node-positive group and control group were 1.61 +/- 3.09 and 16.77 +/- 30.40, respectively (P=0.029). Downregulated expression of miR-99a was dosely related to depth of invasion (DOI) and lymph-vascular space invasion (P<0.05). Univariate analysis revealed that downregulated miR-99a and deeper DOI were associated with worse 5-year disease-free survival, while multivariate analysis showed that only the expression level of miR-99a was an independent factor for disease-free survival (HR =0.120; 95% CI: 0.015-0.979; P=0.048). Patients with downregulated miR-99a tended to have more unfavorable overall survival, but the difference did not reach statistical significance. Conclusions: MiR-99a plays an inhibitory role in the pathogenesis of lymph node metastasis and may serve as a novel prognostic biomarker for patients with CSCC.

Prostate cancer is a kind of common malignant tumor in males’ urinary system, which could seriously threaten men’s reproductive function and life safety. Robot-assisted radical prostatectomy is a new surgical treatment way to prostate cancer, it breaks limitations of age and tumor location on operation, with advantages of smaller trauma, better preservation of sexual function and continence. Different positions in the Da Vinci robot-assisted laparoscopic radical prostatectomy（the supine position with low head and drooping lower legs, the split-leg position with elevated buttocks, the modified split-leg position）, influence of artificial pneumoperitoneum pressure of 10 mmHg, 12 mmHg, 14 mmHg on patients’ body and intraoperative nursing interventions were systematically reviewed in this paper, and the future development of fine nursing was discussed to provide references for surgeons performing similar robot-assisted surgery.

目的:比较一次性可吸收钉皮内吻合器与合成可吸收外科缝线在甲状腺和乳腺手术切口闭合中的应用及愈合情况.方法:选取医院收治的152例行甲状腺和乳腺手术患者,使用中央随机系统随机化分为观察组和对照组,每组76例,观察组采用一次性可吸收钉皮内吻合器行甲状腺和乳腺手术切口闭合;对照组采用传统可吸收缝线行甲状腺和乳腺手术切口闭合.分析比较两组患者术后第8 d切口甲级愈合率,以及单位长度切口缝合时间、切口愈合效果、缝合部位疼痛程度和缝合线和(或)缝合钉吸收等疗效情况,评价一次性可吸收钉皮内吻合器的临床安全有效性.结果:两组患者术中单位长度切口缝合时间观察组短于对照组,两组比较差异有统计学意义(t=9.735,P<0.001);两组患者术后1 d和3 d缝合部位疼痛程度视觉模拟量表(VAS)评分比较,差异无统计学意义(t=0.773,t=0.605;P>0.05);两组患者术后8 d切口甲级愈合率、术后8 d、(42±3)d以及28周(+10 d)缝合线和(或)缝合钉吸收情况进行比较,差异均无统计学意义.结论:一次性可吸收钉皮内吻合器与合成可吸收外科缝线相比具有缝合速度快、实现临床手术切口快速闭合、缩短手术时间和降低医疗成本的优势.

Objective:To explore the development of surgical arrangement and scheduling scheme based on medical and nursing integration and queuing mechanism and to evaluate its application effect.Methods:The patients who completed surgery in Cancer Hospital of Peking Union Medical College in 2018 were set as the control group, and patients who completed the surgery in the same period in 2019 were set as the experimental group. The control group adopted traditional surgical scheduling methods, while the experimental group formulated surgical arrangements and scheduling programs based on the theoretical basis of medical and nursing integration and queuing mechanism and reached consensus on medical and nursing in the program practice. Every department formulated procedures for receiving procedures and submitted surgical information and pre-sequence of requirements. According to the operation information of each ward, Operating Room arranged the operation, the operating rules, the energy level matching and dynamic adjustment of Operating Room nurses, defined rules of receiving emergency surgeries, real-time monitoring and feedback, strictly implemented the established plan and observed its application effect through the utilization rate of the operating table.Results:In 2019, an average of 5.36 cases/d were performed on each operating table, and the utilization rate of the operating table increased from 91.26% to 95.74%.Conclusions:The operation scheduling mode based on medical and nursing integration and queuing mechanism can realize the joint decision-making and cooperation of medical staff, which has certain advantages in improving the utilization rate of the operating table. In the future, artificial intelligence, big data and other methods can be fully utilized to continuously optimize the operation scheduling mode and improve the operating efficiency of the Operating Room.

ObjectivePrognostic nutritional index (PNI), calculated as serum albumin (ALB) (g/L) + 5 × total lymphocyte count (109/L), is initially used to evaluate nutritional status in patients undergoing surgery and may evaluate the therapeutic effects and predict the survival of various solid tumors. The present study aimed to evaluate the potential prognostic significance of PNI in breast cancer patients receiving neoadjuvant chemotherapy (NACT).MethodsA total of 785 breast cancer patients treated with neoadjuvant chemotherapy were enrolled in this retrospective study. The optimal cutoff value of PNI by receiver operating characteristic curve stratified patients into a low-PNI group (<51) and a high PNI group (≥51). The associations between breast cancer and clinicopathological variables by PNI were determined by chi-square test or Fisher’s exact test. Kaplan–Meier plots and log-rank test were used to evaluate the clinical outcomes of disease-free survival (DFS) and overall survival (OS). The prognostic value of PNI was analyzed by univariate and multivariate Cox proportional hazards regression models. The toxicity of NACT was accessed by the National Cancer Institute Common Toxicity Criteria (NCI-CTC).ResultsThe results indicated that PNI had prognostic significance by an optimal cutoff value of 51 on DFS and OS in univariate and multivariate Cox regression survival analyses. Breast cancer patients with a high PNI value had longer DFS and OS than those with a low PNI value [47.64 vs. 36.60 months, P < 0.0001, hazard ratio (HR) = 0.264, 95%CI = 0.160–0.435; 73.61 vs. 64.97 months, P < 0.0001, HR = 0.319, 95%CI = 0.207–0.491, respectively]. Furthermore, the results indicated that patients with high PNI had longer DFS and OS than those with low PNI in early stage and advanced breast cancer, especially in advanced breast cancer. The mean DFS and OS times for breast cancer patients with high PNI by the log-rank test were longer than in those with low PNI in different molecular subtypes. Moreover, the mean DFS and OS times in patients with high PNI by the log-rank test were longer than in those patients with low PNI without or with lymph vessel invasion. The common toxicities after neoadjuvant chemotherapy were hematologic and gastrointestinal reaction, and the PNI had no significance on the toxicities of all enrolled patients, except in anemia, leukopenia, and myelosuppression.ConclusionPretreatment PNI with the advantages of being convenient, noninvasive, and reproducible was a useful prognostic indicator for breast cancer patients receiving neoadjuvant chemotherapy and is a promising biomarker for breast cancer on treatment strategy decisions.

BACKGROUND:Biological matrix-assisted one-stage implant-based breast reconstruction (IBBR) could improve the inframammary fold to achieve good esthetic results. However, whether biological matrix-assisted one-stage IBBR yields better postoperative outcomes compared with two-stage IBBR remains unclear. We aimed to compare and analyze surgical complications and patient-reported outcomes (PROs) based on the BREAST-Q version 2.0 questionnaire between biological matrix-assisted one-stage IBBR and traditional two-stage IBBR.METHODS:From May 2015 to June 2019, eligible patients who underwent SIS matrix-assisted one-stage IBBR or two-stage IBBR were enrolled in this retrospective cohort study. PROs were measured with BREAST-Q version 2.0, which scored the health-related quality of life, satisfaction, and experience domains. Complications were divided into major complications (patients requiring reoperation) and minor complications (patients who could be treated in the dressing room). PROs and complications were compared between the SIS matrix-assisted one-stage IBBR and two-stage IBBR groups. A multivariate linear regression analysis was used to identify the social and surgical factors that affected PROs.RESULTS:At our institution, 124 eligible patients were recruited. Seventy-nine patients (63.7%) underwent SIS matrix-assisted one-stage IBBR reconstruction, and 45 patients (36.3%) underwent tissue expander/implant reconstruction (two-stage IBBR). Postoperative BREAST-Q version 2.0 was completed by 68 of 79 patients (86.1%) in the SIS matrix-assisted one-stage IBBR group and by 35 of 45 patients (77.8%) in the two-stage IBBR group. In the satisfaction-related quality of life domain, satisfaction with breast was 9.27 points higher in the SIS matrix-assisted one-stage IBBR group (p = 0.012) compared with the two-stage IBBR group. The multivariate linear regression analysis showed that implant volume (p = 0.031) and postoperative radiotherapy (p = 0.036) significantly influenced the PRO of satisfaction with breast. However, patients in the SIS matrix-assisted one-stage IBBR group had a higher minor complication rate compared with patients in the two-stage IBBR group (p = 0.026).CONCLUSIONS:Our retrospective study showed that although patients treated with biological matrix-assisted one-stage IBBR tended to have higher postoperative complication rates, this technique correlated with better PROs compared with two-stage IBBR.LEVEL OF EVIDENCE III:This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

OBJECTIVE:To study the expression of LINC00673 in cervical cancer and cervical intraepithelial neoplasia (CIN) and to explore the role of LINC00673 in the development of cervical cancer.METHODS:The expression of LINC00673 in serum from cervical cancer patients, CIN patients, and healthy participants was detected by RT-qPCR. The function of LINC00673 in cervical cancer cells was analyzed using in vitro and in vivo experiments.RESULTS:Our results revealed that serum LINC00673 levels were highest in cervical cancer patients, followed by patients with CIN and healthy controls. In vitro experiments demonstrated that overexpression of LINC00673 enhanced the proliferation and cell cycle progression of HeLa and SiHa cells. In vivo experiments showed that the tumor weight and volume of nude mice subcutaneously injected with LINC00673-overexpressing HeLa cells were larger than those of nude mice injected with control cells (P < 0.05). Western blotting showed that cell cycle-related proteins cyclin A2 and cyclin E and interstitial-associated proteins Snail and N-cadherin were upregulated and p53 signaling pathway-related proteins were downregulated in LINC00673-overexpressing HeLa and SiHa cells.CONCLUSION:LINC00673 plays an important role in the development of cervical cancer and may serve as a new therapeutic target for cervical cancer.

目的:探讨原发性阴道肠型腺癌（PVAIT）的临床诊治要点。方法:收集2015年1月至2019年12月中国医学科学院北京协和医学院肿瘤医院诊治的2例PVAIT患者的临床病理资料，并回顾分析相关文献。结果:2例患者均为早期病变，病灶局限，结合病理特征及影像学检查排除转移性癌，明确诊断为PVAIT。2例PVAIT患者中，1例患者行根治性手术治疗，术后同步放化疗，目前已随访43个月无复发；1例患者化疗后9个月肿瘤复发，复发后未规范治疗，发病48个月进展至Ⅳ期。结论:PVAIT临床罕见，需依靠病理及影像学检查与肠道转移性腺癌鉴别。治疗上应注重个体化，早期、病灶局限者首选手术治疗。