目的 探讨黄精抗肿瘤的药效物质基础及分子作用机制.方法 采用中药网络药理学数据库和分析平台(TCMSP)对黄精的化学成分进行收集,借助计算机辅助预测吸收、分布、代谢和排泄(ADME)性质并结合obioavail 1.1和pre-Caco-2预测模型对活性成分群进行初筛;对所有潜在活性成分进行靶点识别;采用Cytoscape3.6.1软件进行“活性成分-靶标”网络的构建,并利用“network analyzer”插件对网络的拓扑性质进行分析;采用在线分析工具DAVID进行KEGG通路富集分析.结果 黄精中已鉴定的39个化合物,其中18个具有良好的ADME性质的化合物,这些潜在的活性成分中,共有14个活性成分及其对应的19靶标与黄精抗肿瘤的活性密切相关.通过构建“活性成分-靶点”网络及对网络进行分析,共获得个4关键化合物:黄芩素、3'-甲氧基大豆苷元、异甘草素、(2R)-7-羟基-2-(4-羟苯基)-4-苯丙二氢呋喃;2个关键靶标:前列腺素G/H合酶2、热休克蛋白90AA1.在对靶点进行KEGG通路分析时,共获得12条与抗肿瘤相关的通路.结论 黄精可通过多成分、多靶点及多通路的作用机制发挥其抗肿瘤活性.

目的 应用超高效液相色谱联用四级杆飞行时间质谱仪(UPLC-Q-TOF-MSE)结合UNIFI数据平台对补气通络颗粒的化学成分进行快速定性分析.方法 应用时间依赖型MSE法采集样品质谱数据,结合自动检出及人工核对方法分析补气通络颗粒的化学成分.结果 通过UNIFI数据库自动检测结果、搜索在线和离线数据库以及人工核对相关文献,鉴定黄酮类、皂苷类、萜类、酯类、氨基酸和酚酸类共72种化合物.其中,7种来自红芪,14种来自三七,24种来自泽泻,9种来自当归,2种来自川芎,6种来自蝉蜕,6种来自郁金,4种来自桂枝.结论 本方法可快速定性分析补气通络颗粒的多种化学成分,为补气通络颗粒的质量控制、药效物质基础解析、作用机制阐明和组分配伍中药的开发提供参考.

目的 重点整理糖尿病常见并发症的发病机制及其药物治疗的研究成果,对该病的深入探究、临床规范给药及新药研制等明确方向.方法 查阅与梳理国内外相关文献资料,对其进行归纳与研究.结果 对糖尿病临床常见的7类并发症及其药物治疗做了阐述,并对其并发症发病机制和药物治疗做了总结.结论 糖尿病并发症的发病机制非常复杂,通过临床研究来看,应对不同病人的发病机制实施个性化的药物治疗方案,确保临床用药更加科学、规范,真正地为病人的健康安全保驾护航.

目的:建立红花药材中6-羟基山柰酚-3,6,7-三-O-葡萄糖苷、羟基红花黄色素A和脱水红花黄色素B3种化学成分的UPLC含量测定方法,并评价新疆不同主产区红花的品质.方法:采用BEH C18色谱柱(100 mm×4.6mm,1.7 μm),以乙腈(A)-0.5％甲酸溶液(B)为流动相,梯度洗脱;流速为0.1 mL/min;检测波长为275 nm;柱温为30 ℃.结果:6-羟基山柰酚-3,6,7-三-O-葡萄糖苷、羟基红花黄色素A、脱水红花黄色素B3个成分的线性范围分别为3.375～ 108μg/mL、9.76 ～ 312.4μg/mL和2.54～ 81.5 μg/mL,平均加样回收率分别为100.34％、98.96％和96.47％,应用此法测定新疆不同主产区红花药材成分含量,米东区八家户村和新疆昌吉奇台县基地的红花药材中指标成分含量较高.结论:建立的UPLC方法操作简便、快速、结果准确,适合红花药材的品质评价及质量控制.

目的：对冠心病的中医和西医学认识及相关药物治疗概况进行综述，为冠心病的进一步研究和临床合理用药及新药开发提供参考。方法：查阅国内外相关文献，进行总结分析概括。结果：对冠心病的现代医学和中医学认识做了总结，并对相关药物做了列举。结论：冠心病的发病机制是多因素引起的，临床应针对不同患者的发病机制采用选择性的药物进行治疗，提高用药的科学性和合理性，保障患者生命安全。

Guanxin Shutong capsule is a traditional Chinese medicine for the treatment of myocardial ischemia (MI). Previous studies have shown that the formula has four main active ingredients (FMAI), protocatechuic acid, cryptotanshinone, borneol, and eugenol. However, the mechanisms of action of these FMAI against MI injury are still not well known. The aim of the present study was to evaluate the protective effects of the FMAI on MI in vitro and in vivo. In vitro, rat neonatal cardiomyocytes were isolated, the cell viability and apoptosis rate were, respectively, measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and fluorescence activating cell sorter, and the intracellular calcium concentration ([Ca2+]i) and CaM and CaMKII δ mRNA as well as protein levels were determined. Meanwhile, their downstream targets of RyR2 and PLB were also measured by western blot. In vivo, a rat model of coronary artery ligation was used to evaluate the cardioprotective effects. Infarct sizes of heart tissues and levels of serum biochemical indicators, including creatine kinase, lactate dehydrogenase, superoxide dismutase, and glutamate oxaloacetic transaminase, were measured. The in vitro results showed that the FMAI inhibited cell apoptosis, reduced [Ca2+]i, decreased the expression of CaM and CaMKII δ, and increased the expression of RyR2 and PLB. In vivo, the FMAI diminished infract size, reduced creatine kinase, lactate dehydrogenase, and aspartate aminotransferase levels, and enhanced superoxide dismutase activity. In conclusion, our data suggest that the FMAI suppressed calcium overload and exerted its protective effect via its antioxidant, antiinflammatory, and anti-apoptosis activities. Copyright © 2017 John Wiley & Sons, Ltd.

Objective To research the intestinal absorption characteristics of representive ingredients including gallic acid (GC) and protocatechuic acid (PA) in Choerospondiatis Fructus extracts at different intestinal sections and different time.Methods The everted gut sac model was established,and the contents of GC and PA in the intestinal samples were determined by high performance liquid chromatography (HPLC),with calculating the absorption parameters and analysis of the absorption characteristics in the different parts of rat small intestine at different time.Results The final determination point for everted gut sac was 2.5 h,Tyrode buffer solution and other ingredients had no interference to the chromatographic peak of GC and PA.Both GC and PA were linear absorption in various intestinal segments,and the square of the correlation coefficient (r2) value was more than 0.90.The advantageous accumulative absorption of different intestinal parts were duodenum,jejunum,ileum and colon.Conclusion Both GC and PA in the intestinal absorption characteristics were accorded with zero order absorption rate,and duodenum was the most absorpted part.

目的:考察中国药典2010年版红花中山柰素含量测定色谱条件下山柰素含量测定的准确性.方法:利用二极管阵列检测器、液质联用色谱仪及核磁共振仪对山柰素主峰中包埋的干扰成分的组成及结构进行研究.结果:6-甲氧基山柰酚在中国药典2010年版红花质量标准中山柰素含量测定的色谱条件下,对山柰素的测定存在严重干扰,该分析方法不能够准确测定山柰素含量.结论:中国药典2010年版红花质量标准中山奈素含量测定方法的准确性值得商榷.

检索淡竹叶研究相关文献,对淡竹叶发挥清热、除烦、利尿药效作用及其物质基础方面研究进行文献整理和分析,按照现代中药药理学关于中药清热、除烦、利尿作用的认识进行归纳、分析和总结,对临床中淡竹叶清热除烦、利尿用药的合理性作出解释,为更深层次的药学研究和临床合理用药提供依据.

目的：从热分析角度研究天然天竺黄与合成天竺黄异同。方法：采用差热分析法（DTA）和热重法（T G ）对天然天竺黄及合成天竺黄进行分析。结果：合成天竺黄附加水失去温度较天然天竺黄低20℃以上；天然天竺黄在310℃～321℃之间存在明显的挥发分而合成天竺黄在此区间曲线未出现明显变化。结论：天然天竺黄与合成天竺黄热行为存在显著差异，热分析法可作为一种快速评价天竺黄的分析方法。

Objective To establish an HPLC method for the determination of baicalin in Kelu Oral Liquid.Methods The HPLC was performed on the Hibar C18 column(250 mm×4.6 mm,5 μm) with acetonitrile-0.1%phosphoric acid solution(24 ∶76) as the mobile phase at the flow rate of 1.0 mL/min and the detection wavelength of 280 nm.Results The linear relation of baicalin during the range of 0.170 6-1.364 8 μg with the peak area was good and the linear regression equation was Y=3 406 924X+138 099(r=0.999 6).The average recovery rate was 97.31%,RSD was 1.024%(n=6).Conclusion This method is simple,accurate,better reproducible and can be used to improve the quality standard of Kelu Oral Liquid.

目的:建立高效液相色谱法同时测定高冠平胶囊中三七、红参所含皂苷类成分含量的方法。方法:采用高效液相色谱法,色谱柱为HibarC18（250mm×4.6mm,5μm）;流动相A为乙腈,B为水;流速为1.0mL.min-1;检测波长为203nm;洗脱程序:0～35min,A相为19%,B相为81%;35～85min,A相为19%→35%,B相为81%→65%;85～100min,A相为35%→19%,B相为65%→81%。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.4104～3.2832μg、2.0884～16.7072μg、1.222～9.776μg、2.0072～16.0576μg范围内呈良好的线性关系,其回收率分别为98.92%、98.97%、99.01%、99.25%,RSD分别为1.08%、1.06%、0.85%、1.15%。结论:本方法简便、准确、重现性好,可用于高冠平胶囊的质量控制。

目的观察化瘀通脉胶囊对实验性脑缺血大鼠的影响.方法结扎大鼠双侧颈总动脉,建立脑缺血大鼠模型,并观察化瘀通脉胶囊对脑指数、脑含水量、脑血管通透性的影响.结果化瘀通脉胶囊大、中剂量组可明显改善脑缺血所致脑含水量增加及脑血管通透性等病理改变.结论化瘀通脉胶囊确有改善大鼠实验性脑缺血的作用.

目的观察胃达康胶囊对实验性胃溃汤大鼠的影响.方法应用大鼠胃壁粘膜抹酸法用载有醋酸之特制玻管,自大鼠膜胃进入腺胃加酸,制作实验性胃溃疡(胃窦部)模型,并观察胃达康胶囊对溃疡面积、溃疡愈合速度等的影响.结果胃达康胶囊大、中、小剂量组可明显减少实验性大鼠胃溃疡面积,并可提高溃疡愈合速度等病理改变.结论胃达康胶囊确有改善大鼠实验性胃溃疡的作用.

藿香祛暑软胶囊是中药复方制剂,由广藿香、苍术、丁香、白芷等12种药材组成,具有祛暑化湿,解表和中的功效,用于内蕴湿滞、受暑感寒引起的恶寒发热、头痛无汗、四肢酸懒、恶心呕吐、腹痛腹泻,收载于<卫生部药品标准中药成方制剂第二十册>.为了更加有效地控制制剂的质量,本研究增加了丁香、白芷的薄层鉴别,在参考文献的基础上,采用高效液相色谱法对陈皮中所含橙皮苷进行了定量测定,为该制剂的生产提供了质量控制依据.