目的:探讨克罗恩病(CD)患者接受英夫利西(IFX)治疗前后Tim-3/aPKC-ι通路的变化并分析其临床意义和在病程判断中的作用.方法:制备外周血T细胞亚群同时构建靶向RNA干扰Tim-3基因的慢病毒载体,体外转染T细胞亚群,检测细胞因子分泌水平和Tim-3以及aPKC-ι mRNA的变化,确认Tim-3/aPKC-ι通路的存在;收集2009年6月至2019年7月武汉大学人民医院符合研究标准的45例CD患者,分别在第0、2、6周时给予IFX治疗.以同期16例健康体检者为健康对照组.CD患者于治疗开始前(0周)和治疗第5、10和15周时取静脉血,并经结肠镜检查取肠黏膜活组织检查标本.观察CD患者克罗恩病活动指数(CDAI)、血沉(ESR)、C-反应蛋白(CRP)的变化.使用荧光定量PCR方法分析外周血和肠黏膜组织内Tim-3和aPKC-ι mRNA基因表达水平变化.结果:RNA干扰Tim-3基因在T细胞表达后,可抑制下游的aPKC-ι mRNA表达水平以及T细胞增殖的作用,提示CD发病机制中存在Tim-3/aPKC-ι通路.IFX治疗前,CD患者在外周血Tim-3和aPKC-ι mRNA表达水平(5.25±0.89和5.11±0.45)和肠黏膜组织内Tim-3和aPKC-ι mRNA表达水平(5.77±0.56和5.07±0.35)均明显高于健康对照者,差异均有统计学意义(均P＜0.05).第10周和15周时,CD患者外周血Tim-3和aPKC-ι mRNA表达水平和肠黏膜组织内Tim-3和aPKC-ιmRNA表达水平均明显低于治疗前,差异均有统计学意义(P＜0.05).结论:证实Tim-3/aPKC-ι通路在CD的发病机制中有重要作用,活动期CD患者经IFX治疗后Tim-3和aPKC-ι mRNA表达水平下降,提示Tim-3/aPKC-ι通路参与IFX治疗活动期CD,可以导致临床缓解,下降的幅度对病程判断具有临床意义.

目的 探讨人结肠癌细胞SW620中细胞周期蛋白1(CCND1)的表达情况及其异常表达对细胞增殖的影响,阐述microRNA-138(miR-138)在SW620增殖中的可能机制.方法 采用实时荧光定量聚合酶链式反应(qRT-PCR)以及Western blot确定结肠癌组织和癌旁组织中CCND1的表达情况;随后采用qT-PCR检测结肠癌组织内miR-138的表达,并通过统计学分析miR-138与CCND1表达的相关性,培养SW620细胞并转染miR-138模拟物(mimics)后验证miR-138与CCND1的相关性,在SW620细胞内进一步转染miR-138模拟物后,Transwell和MTT检测不同处理组中细胞增殖和迁移能力的改变情况;继续转染CCND1 siRNA后,探索miR-138影响细胞生物学过程的可能机制.结果 结肠癌组织中,与相应癌旁组织相比,CCND1表达显著升高,miR-138表达降低,差异有统计学意义(P<0.05);转染miR-138mimics后,与空白对照组(未转染组)相比,细胞迁徙能力下降,同时增殖降低,差异有统计学意义(P<0.05);转染CCND1 siRNA后,与未转染组相比,miR-138表达变化无显著差异(P>0.05),而细胞侵袭能力和增殖能力较未转染组相比显著改变,差异有统计学意义(P<0.05).结论 结肠癌组织中CCND1异常升高可能与miR-138的表达降低有关,且miR-138影响SW620细胞的迁移和增殖能力可能是通过影响CCND1的表达实现的.

Colorectal cancer (CRC) is the second most commonly diagnosed cancer in females and the third in males. In this work, we aim to investigate the possible anti-cancer effects of interferon-gamma (IFN-γ) in CRC cells. We observed that IFN-γ induced mitochondria-derived reactive oxygen species (ROS) production in a time-dependent manner in SW480 and HCT116 cell lines. The IFN-γ-induced mitochondrial ROS generation was dependent on the activation of cytosolic phospholipase A2 (cPLA2). In addition, a mitochondria-targeted antioxidant SS31 and/or cPLA2 inhibitor AACOCF3 abolished the IFN-γ-induced ROS production and subsequent autophagy and apoptosis. Moreover, suppression of autophagy by CQ was able to reduce IFN-γ-induced cell apoptosis. Beclin-1 gene silencing resulted in caspase-3 inactivation, decreased Bax/Bcl-2 ratio and less population of apoptotic cells. Collectively, our results suggested that IFN-γ induces autophagy-associated apoptosis in CRC cells via inducing cPLA2-dependent mitochondrial ROS production.

目的 探讨高位胆管空肠Roux-en-Y吻合术后吻合口狭窄再次手术的方法及临床意义.方法 回顾性分析武汉大学人民医院19例高位胆管空肠Roux-en-Y吻合术后吻合口狭窄需再次手术治疗病人的临床资料.结果 19例病人均行吻合口重建手术,且术中、术后无死亡病例;术后仅有1例病人出现胆漏,2例病人出现胆管炎.结论 针对胆管空肠Roux-en-Y吻合术后吻合口狭窄再次手术病人的实际情况及狭窄不同程度,结合CT或MRI等影像学检查结果制定针对性治疗方案,有助于提高手术成功率并改善病人恢复情况.

Objective To investigate the therapeutic effect and possible mechanisms of alkannin on inflammatory bowel disease in mice.Methods Sixty-four specific-pathogen-free female Bal/c mice were randomly divided into 8 groups,with 8 mice in each group.The normal control group was given food,water,and 1 ml edible olive oil.The model group was given food,3％ dextran sodium sulfate(DSS) sterile aqueous solution and 1 ml edible olive oil.The 1 mg/kg alkannin(AKN-1) group,2 mg/kg alkannin (AKN-2) group,4 mg/kg alkannin (AKN-4) group,8 mg/kg alkannin(AKN-8) group,16 mg/kg alkannin (AKN-16) group were given food,3％ DSS sterile aqueous solution and 1 ml edible olive oil dissolved with alkannin.The positive control group was given food,3％ DSS sterile aqueous solution and 1 ml edible olive oil dissolved with mesalazine.After 6 days of treatment,colon macroscopic damage index(CMDI),tissue damage index(TDI) were used to evaluate the therapeutic effect.Expression levels of transforming growth factor-β1 (TGF-β1) and interleukin-17 (IL-17) mRNA and protein were tested.Results There were no significant differences of CMDI and TDI scores among AKN-1 group,AKN-2 group and model group(P ＞ 0.05).CMDI and TDI scores in AKN-4 group,AKN-8 group and AKN-16 group were significantly lower than those in model group(P ＜0.05).Expression levels of TGF-β1 mRNA and IL-17 mRNA in AKN-1,AKN-2,AKN-4,AKN-8 and AKN-16 groups were significantly lower than those in model group[TGF-β1 mRNA:(2.90 ±0.33),(2.71 ±0.73),(2.14 ±0.19),(2.06 ±0.35),(1.63 ±0.31) vs (3.88 ±0.58);IL-17 mRNA:(2.48 ±0.35),(2.25 ±0.64),(2.07 ±0.21),(1.78±0.24),(1.67 ±0.23) vs (3.21±0.41)] (P ＜ 0.05).Expression levels of TGF-β1 and IL-17 in AKN-1,AKN-2,AKN-4,AKN-8 and AKN-16 groups were significantly lower than those in model group (P ＜ 0.05).Conclusion Alkannin can change the TGF-β1-helper T cell 17-IL-17 axis to exert anti-inflammatory effect on inflammatory bowel disease in mice.

Objective To evaluate the safety and feasibility of 3D laparoscopic wedge resection (LWR) guided by calibrating bougie for gastric gastrointestinal stromal tumors (GIST) adjacent to the cardia.Methods From January 2014 to December 2015,we performed 3D LWR guided by calibrating bougie on 20 cases of gastric GIST near to (≤5 cm) the cardia.Perioperative records,histopathology results and long-term outcomes of these patients were retrospectively analyzed.Results In all patients given 3D LWR guided by calibrating bougie,there was no tumor rupture,conversion to open access or residual neoplasms of resection margin.The mean procedure duration was 79.2 ± 23.4 min and the mean blood loss was 35.1 ± 17.1 mL.No more complications occurred except 1 case of postoperative abdominal bleeding cured by conservative therapy.No cardia stenosis was seen under the gastroscopy and angiography of the upper gastrointestinal tract 3 months after the operation.All 20 cases were followed up for 12 to 38 months (mean:22.5 months),and none of them had tumor recurrence or metastasis.Conclusions 3D LWR guided by calibrating bougie is safe and feasible for gastric GIST adjacent to the cardia.This procedure fits to the therapeutic criteria of GIST and effectively prevents the cardia stenosis.

Objective: To investigate the effects of recombinant adenovirus-mediated co-transfection of CEA gene and EPO gene on promoting hematopoietic stem cell directly producing erythrocyte vaccine against colon cancer. Method: The expression adenovirus vectors carrying CEA and EPO or green fluorescent protein (GFP) gene were constructed respectively, and recombinant adenovirus carrying CEA, EPO or GFP were packaged and produced respectively. The bone marrow-derived MSCs of mice were isolated and cultured in vitro by anti-CD117magnetic bead separation, and were transfected with CEA (CEA group), EPO (EPO group) or GFP(GFP group), co-transfected with CEA and EPO (CEA and EPO group), or transfected with no virus (controlgroup). The expression of CEA and EPO gene and protein after transfection in supernatant fluid of culture was detected by RT-PCR and western blot in each group. We have checked and obtained the vaccine with co-transfection of CEA gene and EPO gene by cell red line marker antibody CD71 and GPA, then we carried on experiments with the vaccine in vitro and in vivo. Result: We have successfully gathered the hematopoietic stem cells; flow cytometry analysis result showed that there were significant differences before and after purification (P < 0.05). The expression of double genes (CEA-EPO gene) and protein shows CEA-EPO gene were successfully transfected into the hematopoietic stem cells. We have confirmed erythrocyte vaccine with co-transfection of CEA and EPO gene by antibody CD71 and GPA with flow cytometry. The monocytes cytotoxicity on colon cancer cell line CT26 showed that CEA-EPO group was 57.34 ± 1.13% in proportion of 40:1. Compared with CEAg group and EPO group, there was significant difference between them (P < 0.05). In the experimentation of neoplasma format, the size of murine growth of tumor was 0.29 ± 0.11 cm3 differently in the group of CEA-EPO group; the number of murine live time on day was 69 ± 6.91 days. Compared with the group of CEA and plasmid, EPO, the group of CEA-EPO was significant differences in size and live time (P < 0.05). Conclusion: The erythrocyte vaccine with co-transfection of CEA gene and EPO gene has efficient anti-tumor effects on colon cancer. It not only can promote hematopoietic

Objective To evaluate therapeutic effect of tile shape perineal stapled prolapse resection in treatment of rectal prolapse.Methods The clinical data of 31 patients with rectal prolapse underwent tile shape perineal stapled prolapse resection in the Renmin Hospital of Wuhan University from December 2013 to August 2015 were retrospectively analyzed.All the patients with rectal prolapse were performed the tile shape perineal stapled prolapse resection with general anesthesia and (or) epidural anesthesia.The prolapse was completely pulled out and then axially cut open with a linear stapler in the lithotomy position.Finally,the prolapse was resected stepwise with the curved stapler at the prolapse's uptake.Results There were 8 males and 23 females in the 31 patients.The age was (65.8±3.2)years old.There were 14 patients with Ⅱ degree and 17 patients with Ⅲ degree prolapses.The median course was 5.8 years.The 31 patients were followed up for 0.5-7.0 months.Of 31 cases,the 24 cases were cured and 7 cases were better.It was found out that there was a significant difference between the number of axially cut open with a linear stapler and the weight of specimen (P＜0.05).And there was a significant difference between the number of axially cut open with a linear stapler and the recurrence (P＜0.05) too.When the number of axially cut open with a linear stapler were 4 for Ⅱ degree and 3 for Ⅲ degree,the treatment had been proved to be the highest efficacy.Conclusions Preliminary results of limited cases in this study show that tile shape perineal stapled prolapse resection is safe and effective in treatment of rectal prolapsed.Key of operation is that prolapse is completely pulled out and then axially cut open with a linear stapler at some o'clock for 2-4 points and rectal valves are formed.Prolapsed is resected stepwise with curved stapler at prolapse's uptake.When number of axially cut open with a linear stapler are 4 for Ⅱ degree and 3 for Ⅲ degree,it could achieve the best therapeutic effect.

Objective: To assess the clinical application value of 3D laparoscopic surgery in treatment of gastrointestinal stromal tumor in the fundus of the stomach.Methods: The clinical data of 36 patients with gastrointestinal stromal tumor in gastric fundus treated between January 2014 and June 2015 in Renmin Hospital of Wuhan University were retrospectively analyzed. Of the patients, 18 cases underwent 3D laparoscopic resection (3D laparoscopic group) and 18 cases were subjected to open resection (open surgery group). The perioperative variables and postoperative pathological results as well as recurrence and metastasis were compared between the two groups of patients.Results: There were no differences between the two groups in terms of general data such as age, gender, tumor size and BMI (allP>0.05). No statistical difference in operative time was noted between the two groups, while the indices such as intraoperative blood loss, length of the incision, time to postoperative gas passage and length of hospital stay in 3D laparoscopic group were significantly better than those in open surgery group (allP<0.05). No tumor rupture or positive margin was found and no severe complications such as massive hemorrhage, anastomotic fistula and postoperative intestinal obstruction occurred in either group, and the postoperative pathological and immunohistochemical features of the two groups showed no significant difference (allP<0.05). All patients were followed up for 12 to 36 months, and no cases of tumor metastasis and recurrence or death occurred.Conclusion: Using 3D laparoscopic technique in treatment of gastrointestinal stromal tumor in the gastric fundus can significantly minimize the surgical trauma of the patients, and its long-term efficacy is similar to that of open resection.

M cells,with special morphological structure,are a kind of immune cells in the gut,and also a kind of special antigen-transporting cells,and are closely related to the intestinal mucosal immune function.At an early stage of Crohn''s disease(CD),the presence of M cells proliferation and pathological fracture damage of cytology existed.This article will review literatures about M-cell origin,morphological structure,functions,and adherent-invasive Escherchia coli(AIEC)caused CD by M cell-mediated pathway in recent years.

Objective:To investigate the risk factors for bleeding after endoscopic submucosal dissection (ESD) in treatment of gastric gastrointestinal stromal tumor (GIST).Methods:The clinical data of 124 patients with gastric GIST undergoing ESD from February 2011 to May 2017 in Renmin Hospital of Wuhan University were retrospectively analyzed.The risk factors for hemorrhage after ESD for gastric GIST were determined by univariate and multivariate unconditional logistic regression analysis.Results:Of the 124 patients enrolled,postoperative bleeding occurred in 10 cases (8.06％).Results of the univariate logistic regression analysis showed that histories of liver cirrhosis,coronary heart disease,old brain infarcts and atrial fibrillation were significantly associated with bleeding after ESD for gastric GIST (all OR＞ 1,P＜0.05);further multivariate logistic regression analysis revealed that histories of liver cirrhosis,coronary heart disease,old brain infarcts and atrial fibrillation were independent risk factors for bleeding after ESD for gastric GIST (all OR＞1,P＜0.05).Conclusion:The histories of liver cirrhosis,coronary heart disease,old brain infarcts and atrial fibrillation are risk factors for bleeding after ESD for gastric GIST.Therefore,the preoperative assessment should be optimized and the pre-and postoperative management should be enhanced in patients with these risk factors,so as to reduce the incidence of postoperative bleeding.

目的观察小鼠克罗恩病模型外周血单核细胞及结肠组织中白细胞介素(IL) 17、叉头状/翅状螺旋蛋白3(Foxp3)的表达,探讨其在2,4,6-三硝基苯磺酸(TNBS)诱导的克罗恩病小鼠急性炎症中的作用.方法 共40只6～8周龄雌性Bal/c小鼠,采用随机数字表法随机分为两组,20只以TNBS/乙醇溶液灌肠诱导克罗恩病模型(实验组),另外20只小鼠以同等浓度的乙醇氯化钠灌肠(正常对照组).干预1周后行疾病活动指数(DAI),结肠病理组织学评分(TDI)和结肠大体形态损伤指数(CMDI)评价,以实时荧光定量反转录聚合酶链反应(RT-PCR)检测小鼠外周血单核细胞IL-17mRNA、Foxp3 mRNA的表达,采用免疫印迹法检测小鼠结肠组织IL-17蛋白、Foxp3蛋白的表达.结果 与正常对照组相比,实验组的DAI、TDI、CMDI显著升高[DAI(5.41±0.48)比(0.85±0.07),TDI(5.63±0.74)比(0.74±0.08),CMDI(6.88±0.84)比(0.91±0.06),P＜0.01],外周血单核细胞的IL-17 mRNA显著升高[(1.118±0.145)比(1.000±0.000),P＜0.01],与其DAI、TDI及CMDI呈正相关(r =0.875,0.935,0.825,P＜0.01),Foxp3 mRNA显著降低[(0.797±0.271)比(1.000±0.000),P＜0.01],与其DAI、TDI及CMDI呈负相关(r=-0.885,-0.734,r-0.812,P＜0.01),结肠组织IL-17蛋白显著上升[(0.631±0.331)比(0.272±0.112),P＜0.01],与其DAI、TDI及CMDI呈正相关(r =0.765,0.778,0.734,P＜0.01),Foxp3蛋白显著降低[(0.274±0.112)比(0.592±0212),P＜0.01],与DAI、TDI及CMDI呈负相关(r=-0.889,-0.809,-0.851,P＜0.01).结论在TNBS诱导的结肠炎中,Th17/Treg轴的免疫偏倚是导致克罗恩病发生和发展的重要因素.

目的：探讨IL-33对克罗恩病（ CD）小鼠肠组织细胞因子（ IFN-γ、IL-4、IL-10、TGF-β）及转录因子（ T-bet、GATA-3、FOXP3）的影响。方法选择雌性BALB／c小鼠45只，随机分为对照组、TNBS组、IL-33组，每组15只。TNBS组、IL-33组给予TNBS／50％乙醇混合溶液灌肠建立CD模型，对照组仅予生理盐水灌肠。灌肠后TNBS组腹腔注射PBS，IL-33组腹腔注射重组鼠IL-33（rIL-33），对照组腹腔注射PBS。各组于腹腔注射第5天处死，取肠组织行HE染色，观察肠组织病理变化；采用ELISA法检测肠组织上清液IFN-γ、IL-4、IL-10、TGF-β，Western blotting法检测肠组织T-bet、GATA-3、FOXP3蛋白表达。结果与对照组比较，TNBS组肠组织炎症明显；肠组织IFN-γ水平升高，IL-4、IL-10、TGF-β水平降低（P均＜0．05）；T-bet蛋白表达升高，GATA-3、FOXP3蛋白表达降低（P均＜0．05）。与TNBS组比较，IL-33组肠组织炎症相对较轻；IFN-γ水平降低，IL-4、IL-10、TGF-β表达升高（P均＜0．05），T-bet蛋白表达降低，GATA-3、FOXP3蛋白表达升高（P均＜0．05）。结论 IL-33能缓解CD小鼠病情，其机制与抑制Th1细胞分化、诱导Th2和Treg细胞产生有关。

克罗恩病是一种病因尚不清楚的胃肠道疾病,近年来我国克罗恩病的发病率呈上升趋势.白介素-33(IL-33)是近期发现的白介素-1(IL-1)家族中的新成员,其受体是ST2,IL-33/ST2途径在机体多种疾病的发病过程中发挥重要作用,越来越多的研究发现其与克罗恩病的发生也有一定的关系.本文简述了IL-33在克罗恩病中的研究发现.

Objective To investigate the effect of immature dentritic cell(imDC) on the Crohn's mice models induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS).Methods The mouse were randomly divided into 4 groups:normal group,TNBS group,immature dentritic cell (imDC)group,mature dentritic cell (mDC) group.The other three gourps were administered 50％ ethanol/TNBS vain anus except control group,Each group was injected saline or cells via tail vein before and after administration of anus enema.Control group and TNBS group were injected saline.imDC group was injected imDC.mDC-group was injected mDC.Then we observe the general situation of murine.After one week,the disease activity index (DAI),tissue damage index (TDI) and colon macroscopic damage index (CMDI) were evaluated,the colon level of interleukin-10 (IL-10),transforming growth factor-β (TGF-β) determined by enzyme-linked immunosorbent assay (ELISA).The level of colon forkhead/winged helix protein 3 (Foxp3) protein was determined by Western blotting.Results The scores of DAI,CMDI,TDI in TNBS group were 5.420 ± 0.326,4.923 ± 0.226,7.200 ± 0.851,incresed significantly compared with control group (P＜0.01).And the colon tissue level of IL-10,TGF-β protein were (6.103 ± 1.140),(21.164 ±3.252) ng/L,the relatively expression of protein was 0.439 2 ±0.045 9,decresed significantly compared with crotrol group (P ＜ 0.01).ImDC can significantly decrease the scores of DAI,CMDI,TDI and increse the colon tissue level of IL-10,TGF-β,Foxp3 protein (P ＜0.05).But the mDC increased the scores of DAI,CMDI,TDI and decrese the colon tissue level of IL-10,TGF-β,Foxp3 protein (P ＜ 0.05).Conclusion We succsefully induce Crohn' s mice models by TNBS.And obseve the imDC can significantly inhabit intestinal inflammation in Crohn' s mice models and the mechanism of immune tolerance induced by imDC might be inhibit T lymphocytes responsiveness by regulatory T cells.

目的 探讨Tim-2 mRNA在胃癌患者外周血中的表达特点及其临床意义.方法 采集108例胃癌患者及12例良性病变患者的外周血,采用RT-PCR法分别检测其中Tim-2 mRNA的表达情况.所有患者术后随访0.5 ～3年,根据术后复查B超、CT、MRI或再次手术判断是否发生腹膜转移,并明确腹膜转移的发生时间,分析Tim-2 mRNA的表达量与临床各病理参数以及出现胃癌腹膜转移时间的关系,并且采用Logistic回归方法进行多因素分析.结果 Tim-2 mRNA在胃癌腹膜转移中(57例)的表达高于非腹膜转移组和正常对照组,差异有统计学意义(P＜0.05),且与肿瘤大小、侵犯深度、分化程度、Lauran分型和淋巴结转移有关(P＜0.05),在半年和2～3年的胃癌腹膜转移病例的外周血Tim-2 mRNA表达中,半年组(6.02±1.01) copies/μL的表达高于2～3年组(3.48±0.22) copies/μL,差异有统计学意义(P＜0.05),Tim-2 mRNA表达的多因素回归分析显示,Tim-2 mRNA表达与肿瘤大小(X2=9.463,P=0.002),侵犯深度(X2=8.412,P=0.001),分化程度(x2=10.428,P=0.ooo),Lauran分型(x2=9.723,P=0.001)和淋巴结转移(x2=18.423,P=0.000)有关.结论 Tim-2 mRNA在胃癌患者外周血中的表达与肿瘤的侵袭转移密切相关,可以作为预测胃癌腹膜转移的方法,Tim-2 mRNA表达的高低对胃癌术后腹膜转移的早期和晚期预测也有一定的临床意义,优于腹腔灌洗液脱落细胞学的检查.

Objective Investigate the dysregulated expression of T-cell immunoglobulin and mucin molecules-3 (Tim-3) in mice models with different severity of Crohn's disease, Looking for a new type of Crohn's disease blood marker. Methods the Crohn's disease animal model was made by the anus enema using three different concentrations of 2, 4, 6-trinitrobenzene sulfonic acid ethanol solution, 80 mice were randomly divided into four groups:high concentration group(n=10), moderate concentration group(n=50), low concentration group (n=10), control group(n=10), the expressions of Tim-3 mRNA in peripheral blood were detected by RT-PCR at two days, one week, two weeks, three weeks after we got the mice models in moderate concentration group, we did it two weeks later in the other three groups, At the same time, the content of IL-4 and INF-γin peripheral blood were measured by ELISEA, and colon section from mice underwent HE staining. Results Models of Crohn's disease in mice were successfully constructed, The higher concentration result in higher pathology score as well as more severe disease in mice .the expressions of Tim-3 mRNA in peripheral blood were gradually increased in two weeks after we made the mice models and remained stable after two weeks. At stationary phase, the expressions of Tim-3 mRNA were gradually reduced in high concentration group (2.12 ± 0.24), moderate concentration group(1.68±0.18), low concentration group(1.37±0.12) and Control group (1.00±0.00). And the difference was statistically significant(P<0.05). Conclusion The expression of Tim-3 mRNA in Peripheral blood of a mice model with Crohn's Disease was gradually increased during the first 14 days, and then keep stable, at stationary phase, the expression of Tim-3 mRNA is positively correlated with the severity of the disease. It can be a predictor&nbsp;of disease severity and has good stability and sensitivity.

Objective To investigate the expression of Golgi protein 73(GP73) in colorectal carcinoma and normal colorectal tissues, and explore the meaning of GP73 in colorectal carcinoma and the prospect of application in the diagnosis of colorectal cancer. Methods 30 cases of normal colorectal mucosa and 43 cases of colorectal adenoma were examined by immunohistochemical staining. Results The positive expression rate of GP73 in normal colorectal tissue and cancer tissue were 73.33%, 48.84% respectively; there was significant difference between them(P<0.05). Conclusion GP73 protein is highly expressed in normal colorectal tissue, the expression rate is much higher than in cancer tissues, with a significant difference. GP73 is expected to be a protect factor in the development of colorectal tissue.