BACKGROUND:The treatment of giant condyloma acuminatum in immunodeficient children remains a challenge. In immunodeficient children, human papillomavirus infection may progress to extensive condyloma acuminatum (CA. However, 5-aminolevulinic acid photodynamic therapy (ALA-PDT) has shown favorable normal tissue-sparing properties in combination with high rates of lesion control. This case aims to highlight the safety and efficacy of ALA-PDT on giant condyloma acuminatum in an immunodeficient child induced by BCL11B mutation. METHODS:This report examines two affected siblings presenting with combined neurological and dermatological symptoms. Clinical assessments were conducted to evaluate intellectual development and skin manifestations. Genetic testing was performed to identify underlying mutations. The CA lesions were treated with the combined regimen of liquid nitrogen cryotherapy and ALA-PDT (90 mW/cm2, 20 min). RESULTS:The CA lesions achieved nearly complete resolution with an absence of notable side effects by ALA-PDT with cryotherapy. In addition, both siblings exhibited pronounced intellectual developmental disorder alongside atopic dermatitis-like lesions. Genetic analysis revealed a previously unreported pathogenic mutation in the BCL11B gene (c.1585G>T[p.Glu529*]) in both patients. The recommended diagnosis was IDDSFTA with concomitant Hyper-IgE Syndrome (HIES). Notably, the dermatological manifestations showed improvement following treatment with Dupilumab. CONCLUSION:This case provides evidence supporting the safety and potential advantages of ALA-PDT for CA in the immunodeficient pediatric settings. Meanwhile, these findings deepen the understanding of the clinical implications of the neurological gene BCL11B mutation (c.1585G>T[p.Glu529*]) in skin disorders.

BACKGROUND AND OBJECTIVES:Viral warts are benign proliferative skin lesions caused by the human papillomavirus (HPV) and are contagious among humans. Currently, there are no effective treatments to address the high recurrence rate of viral warts, presenting challenges in achieving their complete eradication. The aim of this study was to evaluate the efficacy and safety of curettage combined with 5-aminolevulinic acid photodynamic therapy (ALA-PDT) for the treatment of recalcitrant acral warts. METHODS:Our study was carried out between July 2023 to June 2025, and the clinical data of 50 patients diagnosed with acral warts were retrospectively collected. Patients were assigned to the curettage group (n = 24) or the combination group (n = 26) based on whether they received photodynamic therapy after curettage. The clinical response effects, recurrence rate and adverse reactions of the two groups were observed and compared. RESULTS:The cure rate in the combination group was 80.8% (21/26), which was significantly higher than 45.8% (11/24) in curettage group. The recurrence rate in the combination group was 19.2% (5/26), which was lower than 54.2% (13/24) in curettage group (P < 0.05). After three months of follow-up, moderate pain was the main adverse reaction in both groups, and no systemic adverse reactions were observed. CONCLUSION:Our results suggest that curettage combined with ALA-PDT is promising as a safe and effective therapy for patients with recalcitrant acral warts.

Nail lichen planus is a chronic inflammatory condition that may be isolated to the nail, resulting in longitudinal nail ridging, trachyonychia, and splinter hemorrhages, and may be highly refractory to conventional treatment. The management of nail lichen planus remains challenging because of the lack of standardized treatment guidelines, and the efficacy of existing therapies, both topical and systemic, is often inconsistent or unsatisfactory. Emerging reports have suggested that abrocitinib, a selective Janus kinase 1 inhibitor, may be safe and effective in treating severe nail lichen planus. This report describes the case of a woman in her late 40s with refractory nail lichen planus who was successfully treated with abrocitinib.

The sequences of the shRNA and siRNA oligonucleotides are listed.

JPX could directly bind with YTHDF2 and USP10, respectively. YTHDF2 knockdown increased BMP2 and activated the AKT pathway in cutaneous melanoma.

JPX knockout reduces the proliferation and migration of cutaneous melanoma cells.

Background: Effective management of cutaneous nocardiosis is hampered by the lack of defined treatment recommendations and frequently requires prolonged antibiotic therapy. Case presentation: This report describes a case of cutaneous nocardiosis who was effectively treated in a brief amount of time with three sessions of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in conjunction with antibiotics. Conclusion: ALA-PDT combined with antibiotics may effectively treat refractory cutaneous nocardiosis.

The primer sequences used are listed.

We report two cases of chronic skin ulcers that were misdiagnosed as skin and soft tissue infections and as an inflammatory myofibroblastic tumour before the final diagnosis of epithelioid sarcoma at our institution. Our coverage of this rare but fatal tumour that can cause chronic skin ulcers is critical because early diagnosis and treatment can reduce morbidity and mortality.

Abstract Aberrant long noncoding RNAs just proximal to Xist (lncRNA JPX) expression levels have been detected in multiple tumors. However, whether JPX is involved in melanoma progression remains unclear. Our study showed that JPX expression is significantly increased in melanoma tissues and cell lines. To clarify the effect of JPX on cutaneous melanoma, we successfully generated JPX-overexpressing or JPX-knockdown A375 and A2058 cells. CCK-8, colony formation EdU, Transwell, and cell-cycle phase assays were performed, and subcutaneously implanted tumor models were used to determine the function of JPX in cutaneous melanoma. The results showed that JPX knockdown reduced the proliferation and migration of malignant melanoma cells both in vitro and in vivo. To further elucidate the molecular mechanism of JPX-induced cutaneous melanoma deterioration, we performed RNA pull-down, RNA immunoprecipitation, coimmunoprecipitation, Western blot, and RNA-sequence analyses. JPX can directly interact with YTHDF2 and impede the protection of YTHDF2 from ubiquitin-specific protease 10 (USP10), which promotes its deubiquitination. Thus, JPX decreases protein stability and promotes the degradation of YTHDF2, thereby stabilizing BMP2 mRNA and activating AKT phosphorylation. Overall, our study revealed a novel effect of JPX on YTHDF2 ubiquitination, suggesting the possibility of blocking the JPX/USP10/YTHDF2/BMP2 axis as a prospective therapeutic approach for cutaneous melanoma. Implications: This study highlights the ubiquitination effect of USP10 and JPX on YTHDF2 in cutaneous melanoma, and proposes that the JPX/USP10/YTHDF2/BMP2 axis may be a prospective therapeutic target for cutaneous melanoma.

JPX overexpression induces the proliferation and migration of cutaneous melanoma cells.

Immune checkpoint inhibitors such as anti-PD-1 receptor antibodies have been shown to be effective in patients with advanced gastric cancer. However, there is a growing concern about immune-related adverse events. A case of a patient with gastric adenocarcinoma who developed toxic epidermal necrolysis (TEN) induced by sintilimab and subsequently developed lichenoid dermatitis is reported. TEN was diagnosed according to a history of sintilimab use, clinical symptoms and physical examination. During hospitalization, the patient developed recurrent fever caused by bacteremia and recovered from TEN after anti-infection and anti-inflammatory treatments. However, when TEN was controlled, the patient developed the lesional manifestations of lichenoid dermatitis. To date, no cases of lichenoid dermatitis after TEN have been reported following the use of PD-1 inhibitors.

Queyrat erythroplasia is an intraepidermal squamous cell carcinoma localized on the glans penis or the inner side of the foreskin. It accounts for about 10% of all penile malignancies and up to 33% cases may lead to invasive squamous cell carcinoma and the intraurethral erythroplasia of Queyrat is relatively rare. Treatment of Queyrat erythroplasia present a challenge especially if the proximal urethra is involved. Here, we report a case of intractable Queyrat erythroplasia involving the urethral meatus. This case suggested that 5-aminolaevulinic acid photodynamic therapy is effective and safe in the treatment of Queyrat erythroplasia, which provides a new choice for the patients with Queyrat erythroplasia with poor therapeutic effect.

BACKGROUND:Condyloma acuminatum (CA) of the anal canal is difficult to treat and subject to relapse.. We investigated the effects of aminolevulinic acid-based photodynamic therapy (ALA-PDT) combined with oral acitretin during therapy of refractory CA in the anal canal. We also conducted a clinical retrospective study for the treatment of intra-anal CA.METHODS:A total of 101 patients diagnosed with intra-anal CA were enrolled. All patients underwent liquid nitrogen cryotherapy as a basic treatment until visible wart clearance. Patients were divided into two groups depending on the treatment modality after cryotherapy. ALA-PDT group with 51 patients was given ALA-PDT treatment, and ALA-PDT plus acitretin group with 50 patients was given ALA-PDT treatment combined with oral acitretin. The cure rate, recurrence rate, and adverse reactions of the two groups were analyzed and recorded.RESULTS:The cure rate in the ALA-PDT plus acitretin group was 94% (47/50), which was significantly higher than 80.4% (41/51) in ALA-PDT group. The recurrence rate in the ALA-PDT plus acitretin group was 6% (3/50), which was lower than 19.6% (10/51) in ALA-PDT group (P<0.05).CONCLUSION:ALA-PDT combined with oral acitretin is effective and safe in the treatment of refractory CA in anal canal, and with further study may become an option for these patients.

目的:探讨重组人表皮生长因子凝胶联合红霉素软膏在激光祛斑祛痣术后创面修复的应用价值.方法:收集我院于2018年4月—2020年4月收治的激光祛斑祛痣术后患者104例,随机分为观察组和对照组,各52例.两组患者均进行激光祛斑祛痣术治疗,对照组术后给予红霉素软膏治疗,观察组术后给予重组人表皮生长因子凝胶联合红霉素软膏治疗.对比两组患者的临床疗效、创面修复相关指标及不良反应发生率.结果:观察组总有效率高于对照组(P<0.05).治疗后观察组创面愈合时间、结痂时间、痂壳脱落时间均短于对照组(P<0.05).两组不良反应总发生率比较无统计学差异(P>0.05).结论:重组人表皮生长因子凝胶联合红霉素软膏应用于激光祛斑去痣术后的创面修复疗效较好,能够加速患者的创面修复,且安全性好,应用价值肯定.

Cutaneous blastomycosis is endemic to North America and is often caused by dimorphic fungi with spores that are inhaled, inoculated spores, or hyphae that infect immunosuppressed and healthy people. It is sporadic and described as a universal imitator with morphological manifestations as erythema, nodules, and ulcers. Our case demonstrated a 69-year-old female bitten by her pet dog who was then diagnosed with cutaneous blastomycosis through social history and detailed laboratory examinations. She experienced a prolonged failure with antibacterial treatment, negative stool and tissue culture, and chronic inflammatory cell infiltrates on tissue pathology. High-throughput sequencing was performed and showed evidence of Blastomyces dermatitidis aetiology. Photodynamic therapy combined with oral itraconazole was administered, and the patient recovered in a short time. Our case presents inoculated cutaneous blastomycosis and a treatment approach in which photodynamic therapy combined with oral itraconazole significantly reduced the duration of disease treatment and affords a promising choice for the treatment of cutaneous blastomycosis.

目的 观察液氮冷冻联合光动力疗法(PDT)治疗儿童尖锐湿疣的疗效及复发率.方法 对23例尖锐湿疣患儿进行液氮冷冻联合PDT治疗,达到1个疗程后观察疣体清除情况,进行至少6个月的随访.结果 经过1个疗程PDT治疗后,患儿痊愈率达91.30％,复发率8.70％,未见严重不良反应.结论 液氮冷冻联合PDT治疗儿童尖锐湿疣简单、有效、创伤小、复发率低,是治疗儿童尖锐湿疣的一种有效的方法.

Condyloma acuminatum is a sexually transmitted disease caused by human papillomavirus infection, which mostly occurs in the external genitalia, vulva, perianal, and the Intraurethral condyloma acuminatum is relatively rare. Treatment of urethral warts present a challenge especially if the proximal urethra is involved. Here, we report a case of intractable urethral condyloma acuminatum involving the urethral meatus and mid penile urethra with scar. This case suggested that local Photodynamic therapy in condyloma of the whole anterior urethra appears to be not only safe and effective, but also might be able to improve existing scars.