细胞焦亡(pyroptosis)是近年来新发现的一种程序性细胞死亡方式,多项研究表明焦亡参与了肿瘤、自身免疫性疾病、感染等多种疾病的病理过程.心脏移植排斥反应主要病理过程是抗原提呈细胞等固有免疫细胞捕获外来抗原并活化T细胞等获得性免疫细胞,活化后的T细胞通过直接杀伤或激活巨噬细胞等天然免疫细胞参与移植物的排斥反应.越来越多的证据表明焦亡可能参与到心脏移植排斥反应过程,本文将针对焦亡在心脏移植排斥中潜在作用的研究进展进行阐述,加深对移植排斥反应的认识,通过靶向焦亡的相关通路为临床治疗提供新策略.

高超声速边界层转捩控制一直是空气动力学研究的热点.粗糙元延迟边界层转捩作为近些年才开始研究的内容,目前仍有诸多现象与机理尚未探究清楚.借助于华中科技大学Φ0.25 m Ma6 Ludwieg管风洞,本文研究了不同位置单粗糙元、多粗糙元与在不同来流情况下粗糙元对边界层转捩以及第二模态不稳定波发展的影响.风洞实验使用PCB132系列高频压力传感器获取尖锥壁面压力脉动信息,通过傅里叶积分变换与互相关分析获取压力脉动功率谱密度与第二模态不稳定波传播速度,对PSD数据计算获取第二模态不稳定波增长率.结果表明,当第二模态不稳定波经过粗糙元时,不稳定波幅值会有明显的减小,但其频段不稳定波的增长率会增大并最终导致转捩的结束点被提前.同时,粗糙元对第二模态不稳定波传播速度无明显影响.

目的:探索内镜下球囊扩张术联合胆道细胞刷检应用于胆管癌的诊断价值。方法:单中心前瞻性研究，本研究在华中科技大学同济医学院附属武汉市中心医院进行，总结2018年1月-2020年1月期间影像学(CT或MRI)提示胆管恶性狭窄可能或原因不明胆管狭窄并同意行内镜逆行胰胆管造影诊治的患者的临床资料及随访情况。所纳入研究患者均行内镜下胆管狭窄球囊扩张术，并于球囊扩张前后分别进行细胞刷检术，扩张前留取病理组织为对照组样本，扩张后留取病理组织为试验组样本。试验组、对照组样本经同一名病理专业副主任医师阅片后提出病理诊断意见。若有癌细胞或显著异型细胞发现提示刷检阳性。若未见癌细胞或异型细胞则提示刷检阴性。阴性患者中结合临床仍高度考虑胆管癌且同意手术，术后大体标本证实为恶性的考虑刷检假阴性；难以判断胆管癌患者经随访2个月有进展考虑刷检假阴性。2个月随访无进展，暂考虑胆管良性狭窄可能。应用优势性检验(McNemar检验)分析试验组与对照组之间是否存在差异。结果:共纳入符合条件的研究病例39例，男性26例，女性13例，年龄(68.0±5.2)岁。经细胞学检测、手术病理及临床随访最终明确诊断胆管癌35例。对照组刷检阳性17例，细胞刷检敏感率48.6%(17/35)，试验组刷检阳性26例，细胞刷检敏感率74.2%(26/35)。另外有2例患者对照组刷检阳性，试验组刷检阴性。合计对照组及试验组刷检阳性共28例，细胞刷检敏感率80.0%(28/35)，两组比较差异有统计学意义( P<0.05)。 结论:内镜下球囊扩张术联合细胞刷检可提高胆管癌的病理诊断灵敏度，球囊扩张前后分别进行细胞刷检可提高诊断灵敏度。

高超声速边界层层/湍流转捩是高超声速飞行器气动力和气动热设计中的难点和热点问题.为了降低开展高超声速边界层不稳定性与转捩实验研究的门槛,研究基于Ludwieg管原理设计并建造了一座Mach 6高超声速管风洞,重点对Ludwieg管风洞的启动和运行过程开展了数值模拟,分析了储气段弯管布局对试验段流场的影响;之后,对该高超声速风洞的自由来流品质进行了静态和动态的标定,验证了风洞的设计Mach数,并给出了流场的动态扰动特征;最后,基于7°半张角尖锥标模开展了高超声速边界层转捩实验,通过表面齐平式安装的高频PCB传感器获得边界层不稳定波,分析了高超声速边界层不稳定波的演化特征.以上工作表明,Ludwieg管相对常规高超声速风洞具有建设和运行成本低、运行效率高、流场品质好等优点,适合开展高超声速边界层转捩等基础实验研究.

目的 探讨溃疡性结肠炎(UC)患者的血小板(PLT)计数及其部分形态学参数指标的临床意义.方法 收集UC患者132例(UC组)及接受结肠镜检查的功能性肠病患者208例(对照组),比较其PLT计数、平均血小板体积(MPV)、大血小板比率(LPLT％)、C反应蛋白(CRP)、纤维蛋白原(Fib)及血浆D-二聚体(D-D)水平差异,并对其与疾病分型和疾病活动度的相关性进行分析.结果 UC组患者PLT计数明显高于对照组,MPV、LPLT％明显低于对照组(P<0.05).UC组患者的CRP水平与炎症累及结肠范围呈正相关(P<0.05),PLT计数和MPV与疾病分型无明显相关性(P>0.05).UC患者的CRP水平和PLT计数与UC活动度呈正相关,MPV与UC活动度呈负相关(P<0.05).UC组患者D-D水平明显高于对照组(P<0.05),两组Fib比较差异无统计学意义(P>0.05).结论 PLT计数和MPV可提示UC患者的疾病活动度,有助于对UC病情进行更加全面的判断.

目的:观察香砂六君子汤合四磨汤加减治疗功能性腹泻(FD)肝气乘脾证的临床疗效及对患者血清胃泌素(GAS)及血浆胃动素(MTL)、血管活性肠肽(VIP)水平的影响.方法:选取72例FD肝气乘脾证患者,按随机数字表法分为对照组和治疗组各36例.对照组给予马来酸曲美布汀片联合双歧杆菌四联活菌片治疗,治疗组给予香砂六君子汤合四磨汤加减治疗.比较2组临床疗效及治疗前后胃肠激素水平、临床症状评分.结果:治疗组总有效率为91.67％,明显高于对照组的66.67％,差异有统计学意义(P＜0.05).治疗后,2组血清GAS水平均较治疗前升高(P＜0.05),血浆MTL、VIP水平均较治疗前降低(P＜0.05);治疗组血清GAS水平高于对照组(P＜0.05),血浆MIL、VIP水平均低于对照组(P＜0.05).治疗后,2组腹痛、腹胀、腹泻评分及症状总分均较治疗前降低(P＜0.05),治疗组腹痛、腹胀、腹泻评分及症状总分均低于对照组(P＜0.05).结论:香砂六君子汤合四磨汤加减治疗FD肝气乘脾证,可改善患者的临床症状,调节胃肠激素水平,疗效优于西药治疗.

Objective:Calcium channel blocker (CCB) is commonly used in the treatment of cardiovascular diseases. It is reported that the prevalence of drug-induced gingival overgrowth (DIGO) is 7.3%-77.6%. The results obtained from some studies are quite different. The purpose of this study was to clarify the relationship between CCB and DIGO and its influencing factors through systematic reviews and Meta-analysis.Methods:Two independent researchers systematically searched PubMed, Cochrane Library, CBM (China Biology Medicine) , CNKI, and Wanfang for clinical studies of DIGO caused by CCB (published from January 1, 1984 to November 30, 2019; no language restrictions) . With each eligible study data extracted, the relative risk (RR) and 95% confidence interval (95% CI) were used for the analysis of systematic review to assess the probability and risk factors of gingival hyperplasia caused by CCB.Results:We obtained 921 articles through searching and 13 articles were included in the Meta-analysis (n = 4432 participants) . The Meta-analysis results showed that the probability of gingival hyperplasia in subjects taking CCB was 4.55 times that of those without CCB [RR = 4.55, 95% CI (3.25, 6.36) , I2 = 57%, P = 0.006]. The results of the subgroup analysis and the Meta-regression analysis showed that the ′Region′ and ′Diagnostic criteria for gingival hyperplasia′ were the sources of heterogeneity among studies. Different gingival hyperplasia diagnostic criteria have different combined RR values, and the combined RR value among domestic studies [RR = 5.83, 95% CI (4.11, 8.26) ] was significantly higher than that among foreign studies [RR = 2.91, 95% CI (1.98, 4.29) ].Conclusions:Taking CCB can lead to gingival hyperplasia. ′Region′ and ′Diagnostic criteria for gingival hyperplasia′ are the main sources of research heterogeneity. Patients taking CCB in China may have a higher risk of gingival hyperplasia, which may be related to poorer periodontal health of the patients. At the same time, factors such as gingival index, bleeding index, and plaque index were related to DIGO caused by CCB. Risk factors in different studies may be inconsistent. Patients who need to take CCB should have periodontal treatment before taking the medicine, which may prevent the occurrence of drug-induced gingival hyperplasia. Periodic periodontal examinations and related periodontal treatments are recommended to be performed regularly to avoid or reduce the degree of DIGO and avoid serious consequences such as loose tooth.

[目的]比较肝硬化门静脉血栓合并食管胃静脉曲张(gastroesophageal varices,GOV)的患者,经颈静脉肝内门体分流术(transjugular intrahepatic portosystemic shunt,TIPS)或内镜下组织胶联合硬化剂注射术行2级预防后,门静脉血栓的变化.[方法]以肝硬化门静脉血栓合并GOV,接受TIPS 10例(TIPS组)和内镜下组织胶联合硬化剂注射术12例(内镜组)进行2级预防的患者为研究对象,搜集临床资料,比较2组患者门静脉血栓的变化.血栓变化评定为完全溶解、部分溶解、血栓稳定和血栓增加;完全溶解和部分溶解归为有效,血栓稳定和血栓增加归为无效.[结果]术后半年,TIPS组血栓变化有效7例,无效3例,有效率70.0％;内镜组有效0例,无效12例,有效率0％;TIPS组门静脉血栓变化有效率与内镜组相比较,差异有统计学意义(P＜0.05).[结论]肝硬化门静脉血栓合并GOV患者,选择TIPS行2级预防后门静脉血栓改变的有效率优于内镜下组织胶联合硬化剂注射术.

目的:观察吡咯生物碱相关肝窦阻塞综合征(PA-HSOS)患者的数字减影血管造影(DSA)图像特征,为PA-HSOS的早期诊断提供依据.方法:选取5例PA-HSOS予以经颈静脉肝内门体分流术(TIPS)治疗的患者作为研究组,选取同期肝硬化门静脉高压行TIPS治疗的10例患者作为对照组,分析和比较两组患者临床资料、腹部增强CT影像DSA下门静脉系统图像,明确PA-HSOS患者的DSA图像特征.结果:PA-HSOS患者均存在不同程度的肝损伤,但是肝损伤程度较轻;PA-HSOS患者增强CT多见肝脏肿大、密度不均一、实质强化不均匀,脾脏不肿大,腹水,门静脉不宽,胃冠状静脉未见明显显示或纤细.而肝硬化患者肝脏多见体积明显缩小,肝脏实质强化均匀,门静脉明显增宽,胃冠状静脉可见.PA-HSOS患者DSA下门静脉未见明显增宽,门静脉呈枯树枝样改变,细小末梢未见明显显示,肝内门静脉未见节段性扩张,胃冠状静脉未见显示或者纤细.而肝硬化患者门静脉显著增宽,肝内门静脉节段性扩张,门静脉末梢可见显影,胃冠状静脉明显可见,脾静脉迂曲.结论:PA-HSOS患者DSA下门静脉未见明显增宽,细小末梢未见明显显示,肝内门静脉呈枯树枝样改变,门静脉未见节段性扩张,胃冠状静脉未见显示或者纤细,是PA-HSOS患者的一个特异征象,DSA下门静脉系统图像特征将为PA-HSOS的诊断提供重要依据.

目的 探讨胰腺分裂症(PD)内镜诊治的常规及补救方法、临床疗效和安全性.方法 回顾性分析该院2014年1月-2016年12月经内镜下逆行胰胆管造影(ERCP)诊治的17例PD患者的临床病历资料,并电话回访患者症状改善及术后恢复情况.结果 17例PD患者,仅1例进行了再次内镜下治疗,共进行内镜下治疗18例次.内镜下治疗常规策略包括:副乳头括约肌切开术(MiES)、副胰管支架置入术(EDSi)及副乳头括约肌扩张术(MiED).术中16例次手术过程顺利,2例次经过困难,但最终采用补救方法联合超声内镜(EUS)对接技术使手术成功.术后并发出血1例次,并发支架相关性胰腺炎2例次,无穿孔和发热等其他并发症发生.17例患者无1例失访,随访时间(16.3±9.4)个月,所有患者术后腹痛视觉模拟评分(VAS)较术前明显改善[(2.0±1.2)vs(7.0±1.8)分],差异有统计学意义(P<0.05).结论 内镜诊治PD具有安全、有效、创伤及并发症少等特点,可明显改善患者临床症状,值得临床推广.根据临床情况可选择常规方法诊治,对于插管困难患者,可联合EUS通过对接技术进行补救诊治.

病例1 患者女,82岁.因"腹痛3 h"收入院.既往有胆囊结石、胆囊炎病史5年,10年来有多次胰腺炎发作病史,有高血压、腔隙性脑梗塞、高脂血症病史.入院查体:急性痛苦面容,巩膜无黄染,心肺(-),腹软,中上腹压痛,无反跳痛,肠鸣音减弱.入院后查血常规白细胞9.45×109/L,血红蛋白104 g/L,中性粒细胞百分比(N%)90.19%,肝功能正常,血淀粉酶1 198.9U/L,脂肪酶860.6 U/L.上腹部CT提示胆囊多发结石,慢性胆囊炎;慢性胰腺炎.MRCP 示胆囊多发结石并胆囊炎;慢性胰腺炎并主胰管明显扩张;完全型胰腺分裂症.临床诊断为慢性胰腺炎急性发作,胰腺分裂症.入院后给予禁食、胃肠减压、抑制胰酶分泌、抑制胰酶活性、抑酸、抗炎、补液等治疗,第6天行ERCP,术中未能发现副乳头及开口,遂经EUS引导下以19 G穿刺针穿刺胰腺尾部主胰管,造影证实进入主胰管后循穿刺针插入导丝,后者沿主胰管经副乳头进入肠腔,十二指肠镜下用圈套器捕捉导丝形成对接,ERCP 示背侧主胰管与腹侧主胰管无交通,行副乳头切开,取石球囊取出些许白色蛋白栓结石,后置入单猪尾胰管支架(7 Fr,15 cm)及鼻空肠管(图1A~图1F),术中诊断:1.胰腺分裂症(完全型);2.慢性胰腺炎,胰管泥沙结石.术后未出现胰腺炎、穿孔及出血等并发症,1周后患者症状完全缓解出院.术后至今随访14个月,患者症状无复发.

患者女,55 岁,因“咳嗽1 周”收入华中科技大学同济医学院附属武汉中心医院呼吸内科?患者精神?饮食?睡眠尚可,大便次数增多,2～5 次/ d,不成形,小便正常,体力?体重较前无明显变化?患者10 余年前有阑尾炎外科手术切除史?入院后完善相关检查,血常规:嗜碱性细胞百分数1. 5%,淋巴细胞百分数42. 16%,嗜酸性细胞百分数5. 47%,中性粒细胞百分数42. 37%,单核细胞百分数8. 47%;生化:谷胺酰转肽酶111. 0 U/ L,谷丙转氨酶66. 0 U/ L,载脂蛋白B1. 03 g/ L,载脂蛋白A 1. 28 g/ L, 低密度脂蛋白胆固醇3. 18 mmol/ L,高密度脂蛋白胆固醇1. 02 mmol/ L,血清总胆固醇5. 69 mmol/ L,甘油三脂2. 17 mmol/ L;甲状腺功能:甲状腺素61. 98 nmol/ L,游离三碘甲状腺原氨酸3. 29 pmol/ L;超敏C-反应蛋白?降钙素原?血液流变学测定未见明显异常;过敏原测定:总IgE 阳性;尿常规:白细胞(±)?隐血(+);大便隐血弱阳性;胸部CT 平扫未见明显异常;肠镜:乙状结肠(距肛缘约17 cm)见一大小约0. 4 cm×1. 5 cm 指状息肉,表面稍糜烂(图1),诊断:乙状结肠息肉,内痔?消化内科会诊后转入消化内科行EMR 治疗?术后病理提示:(结肠)管状腺瘤,上皮呈低级别上皮内瘤变(图2)?

目的 观察长链非编码RNA(lncRNA)BANCR在胃癌细胞中的表达变化,及其对细胞增殖、迁移能力的影响,并探讨其机制.方法 采用RT-PCR法检测胃癌细胞AGS、BSG823、MGC-803、Hs746T和正常胃上皮细胞GES-1中的lncRNA BANCR.将AGS细胞分成LV-BANCR shRNA组、LV-NC组及BLANK组,采用Lipo-fectamine2000分别转染pcDNA3.1-LV-BANCR shRNA、pcDNA3.1-LV-NC及PBS;分别于培养0、1、2、3、4 d后采用MTT试验检测细胞增殖能力,采用细胞划痕试验检测细胞迁移能力,采用Western blotting法检测细胞中的核转录因子NF-κB1p50、NF-κB1p105及磷酸化细胞外信号调节激酶(p-ERK).结果 AGS、BSG823、MGC-803、Hs746T细胞中lncRNA BANCR相对表达量高于GES-1细胞(P均<0.05).培养3、4 d后,LV-BANCR shRNA组OD值小于LV-NC组和BLANK组(P均<0.05).LV-BANCR shRNA组划痕愈合率低于LV-NC组(P<0.05).LV-BANCR shRNA组NF-κB1p50、NF-κB1p105、p-ERK相对表达量均低于LV-NC组(P均<0.05).结论 lncRNABANCR在胃癌细胞中高表达.沉默lncRNA BANCR表达可抑制胃癌细胞的增殖、迁移能力,其机制可能与NF-κB1 p50、NF-κB1 p105及p-ERK表达下调有关.

Reducing sympathetic neurohormone expression is a key therapeutic option in attenuating cardiac remodelling. Present study tested the feasibility of attenuating cardiac remodelling through reducing sympathetic neurohormone level by partial cardiac sympathetic denervation in a rat model of chronic volume overload. Male Sprague‐Dawley rats were randomized into sham group (S, n = 7), aortocaval fistula group (AV, n = 7), and aortocaval fistula with bilateral sympathetic stellate ganglionectomy group (AD, n = 8). After 12 weeks, myocardial protein expression of sympathetic neurohormones, including tyrosine hydroxylase, neuropeptide Y, growth associated protein 43, and protein gene product 9.5, were significantly up‐regulated in AV group compared to S group, and down‐regulated in AD group. Cardiac remodelling was aggravated in AV group compared to S group and attenuated in AD group. The myocardial deposition of extracellular matrix, including collagen I and III, was enhanced in AV group, which was reduced in AD group. Myocardial angiotensin II and aldosterone expressions were significantly up‐regulated in AV group and down‐regulated in AD group. Our results show that bilateral sympathetic stellate ganglionectomy could attenuate cardiac remodelling and fibrosis by down‐regulating sympathetic neurohormones expression in this rat model of chronic volume overload.

Objective To investigate the relationship between serum adiponectin level and risk of colorectal cancer, and to explore the effect of recombinant adiponectin on the proliferation and apoptosis of colorectal cancer HCT116 cells. Methods Serum adiponectin levels in patients with colorectal cancer and healthy controls were dectected by ELISA. With the level of adiponectin as a risk factor, ROC curve was acquired using SPSS software. HCT116 cells were treated with recombinant adiponectin (2.5, 5, 10, 20 μg/mL). Then cell proliferation activity was detected by MTT assay. Cell cycle and apoptosis were detected by flow cytometry. The protein expressions of p21, NF-κBp65, phosphorylated NF-κBp65 (p-NF-κBp65), cyclin D1 and cleaved caspase 3 (c-caspase-3) were tested by Western blot analysis. Results The levels of serum adiponectin in the patients were significantly lower than those in the healthy controls. When the level of adiponectin was used as a risk factor, the area under ROC curve was 0.887 (95% CI: 0.807-0.966). Recombinant adiponectin inhibited the survival rate of HCT116 cells in a time- and dose-dependent manner. After the treatment with recombinant adiponectin, the percentage of HCT116 cells in G1/G0 phase increased and the expression of p21 was upregulated, while the expressions of p-NF-κBp65 and cyclin D1 were down-regulated. Meanwhile, the expression of c-caspase-3 increased and the percentage of apoptotic cells also increased. Conclusion The decreased level of serum adiponectin is closely related to the risk of colorectal cancer. Recombinant human adiponectin can inhibit the proliferation of colorectal cancer HCT116 cells, induce cell arrest at G1/G0 phase and promote apoptosis, which may be related to the down-regulation of NF-κB, cyclin D1, and activation of p21 and caspase-3.

消化道早癌及癌前病变的诊治是当前消化内镜学科的热点,并已造福了很多患者及家庭. 笔者从近十年的ESD操作经历中小结一些体会,分享与读者. 1 诊断 要想顺利开展ESD工作,并使患者最大化收益,诊断很重要. 早癌的诊断知识较庞杂,操作技巧也需在实践中不断提高,其核心理论是内镜表现与病理之间的联系. 首先对于早癌的定义来说就有一个变迁过程,既往的早癌定义为局限于黏膜层和黏膜下层,无论有无淋巴结转移. 最近早癌的定义倾向于局限于黏膜层而无论淋巴结转移,笔者认为这样定义也有利于更加安全有效地开展早癌的ESD治疗.

Objective To study the mechanism of right ventricular pulmonary artery coupling by fitting the pressure volume loop of pulmonary arterial hypertension patients and calculating the related parameters.Methods During January of 2015 to December of 2015,34 patients with pulmonary hypertension and 5 patients with patent oval foramen(PFO)were admitted in Wuhan Asian Heart Hospital,. Inclusion criteria for the pulmonary hypertension group were in accordance with the newest European guidelines for pulmonary hypertension . All of the patients were conducted invasive pressure catheter intervention and MRI. All the pressure data were digitized by GetData software and the CMR tools3D was used to derive right ventricular volume data, fitting the pressure volume loop and calculate the Pmax, Ees, Ea and other related parameters. 5 patients with PFO and 34 patients with pulmonary hypertension were divided into three groupswhich included:the control group (i.e. the patients with PFO), patients with pulmonary artery mean pressure less than 50 mmHg as group A and pulmonary artery mean pressure greater than or equal to 50 mmHg as group B. The pressure volume loop of the three groups were compared and the related parameters were calculated.Results The right ventricular Pmax were(53.05±12.87) mmHg, (134.73±26.38) mmHg, (207.88±65.67) mmHg, in the control group, group A and B respectively. There were significant differences when compared among the 3 groups. There was no statistical difference in Ees between the control group and group A(P>0.05). The Ees of group B was significantly higher than the control group[(1.53±0.97)vs.(0.60±0.28),P<0.05]. The Ea of group B was higher than the control[(1.34±0.74) vs.(0.39±0.15),P<0.05]. The overall ratio of Ees/Ea was lower in group B as compared to the control group [(1.12±0.47)vs.(1.62±0.51),P<0.05].Conclusions In the early stage of pulmonary hypertension, right ventricular contractility preserves and can overcome the slightly elevated afterload .The right ventricle and pulmonary artery coupled with good work. With the increasing mean pulmonary artery,the afterload of right ventricular increases and right ventricular contractility needs further increase to fight against the elevated afterload. The elevation of contractility cannot overcome the change of afterload, resulting in the off paired of mechanical efflciency, causing the right ventricle- pulmonary artery coupling decreases.

Objective To investigate the expression difference of chemokine receptor CCR7 in 62 cases of liver cancer and explore the correlation between CCR7 expression and clinical biological characteristics of liver cancer.Methods Real-time PCR, Western blotting and immunohistochemistry were used to examine the expression and distribution of CCR 7 in liver cancer tis-sues and their adjacent tissues,HepG2 cells and L02 cells.Furthermore,the correlation between the expression difference of CCR7 and clinicopathological characteristics of liver cancer was analyzed.Results Immunohistochemistry showed that CCR7 protein was located in cytoplasm.In addition,the positive expression rate of CCR7 in tumor-adjacent tissues and liver tissues was 24.2%(15/62)and 66.1%(41/62),the difference was statistically significant(χ2 =22.013,P<0.05).CCR7 mRNA levels were significantly lower in tumor-adjacent tissues than in liver tissues[(0.512 ± 0.109)vs.(1.591 ± 0.221),t= -34.403,P<0.01]and lower in L02 cells than in HepG2 cells[(0.485 ± 0.106)vs.(1.768 ± 0.281),t= -33.629,P<0.01].While CCR7 protein expression levels were lower in adjacent tissues than in liver tissues and lower in L 02 cells than in HepG2 cells(all P<0.05).Moreover,the expression difference was correlated with tumor size,portal vein tumor thrombosis,Child-Turcotte-Pugh, extrahepatic metastasis,Barcelona Clinic Liver Cancer(BCLC)classification(P<0.05),but not with gender,age,tumor number, hepatitis B virus,cirrhosis of the liver and serum AFP of patients(P>0.05).Conclusion The CCR7 protein expression is sig-nificantly increased in liver cancer tissues as compared with its adjacent normal tissues.Additionally,CCR7 may be a good mark-er to evaluate the development,invasion and metastasis of liver cancer.

Autoimmune diseases are caused by various factors,the main reason is the interaction between genetic susceptibility and environmental factors,but the pathogenesis is not clear.Recent studies show that the single nucleotide polymorphism (SNP) of protein tyrosine phosphatase nonreceptor type 22 (PTPN22) gene,as a genetic susceptibility factor,is associated with the autoimmune diseases,and is different in the geographical and ethnic groups of the population.This paper expounded the difference of the various gene polymorphisms of PTPN22 in genetic characeristics of different populations.

Objective:To investigate the miRNA-455-3p expression and its action on proliferation and apoptosis in gastric cancer cells.Methods:The miRNA-455-3p expression levels in normal gastric mucous cell line RGM-I and five different types of gastric cancer cell lines (AGS,Hs746T,MGC-803,SGC-7901 and BSG-823) were determined by qRT-PCR method.In gastric cancer cells after transfection with miRNA-455-3p mimics,the cell proliferation was measured by CCK8 assay,the apoptosis was assayed by flow cytometry,the expressions of p27 kip1 and p21 were determined by Western blot analysis,and the activities of the caspase enzymes were analyzed by spectrophotometric method,respectively.Results:The miR-455-3p expression levels were significantly decreased in all the five types of gastric cancer cells compared with RGM-1 cells,which was most evident in AGS cells (all P＜0.05).in AGS cells after transfection with miR-455-3p mimics,the proliferative ability was decreased,the apoptosis rate was increased,the p27 kip1 protein expression level was up-regulated,and the relative activities of caspase-3 and-9 were increased significantly (all P＜0.05),while the p21 protein expression level and caspase-8 activity showed no significant changes (both P＞0.05).Conclusion:miRNA-455-3p expression is down-regulated in gastric cancer cells,up-regulating its expression can inhibit proliferation and induce apoptosis in gastric cancer cells and the mechanism may be associated with its increasing p27 kip 1 expression and enhancing caspase-3 and-9 activities.