Objective: To explore the effects of insulin-like growth factor-1（IGF-1） on incision pain and spinal inflammation in rats. Methods: A total of 30 SD rats, weighing 200-250 g, aged 8-10 weeks,were randomly divided into three groups: control group（group C）, operation group（group O）, and intrathecal injection of IGF-1 receptor antagonist after operation group（group I）. We established the plantar incision pain model in group O rats and injected intrathecally with 10 μL saline 6 hours after operation; we established the plantar incision pain model in group I rats and injected intrathecally with 50 μg IGF-1 receptor antagonist 6 hours after operation; we only injected intrathecally with 10 μL saline in group C rats. Mechanical withdrawal threshold was evaluated one day before operation and 2,4, 12, and 24 hours after operation, respectively. The spinal protein expression of GFAP, IGF-1,IL-1β, and TNF-α was detected by using Western blot and immunofluorescence 24 hours after operation. Results: Compared with that respectively in group C, the mechanical withdrawal threshold began to decrease in group O two hours after operation, the astrocytes in the spinal dorsal horn were activated, the number of IGF-1 positive cells in the spinal dorsal horn increased, and the spinal expression of IL-1β and TNF-α was increased（P＜0. 05）. Compared with that respectively group O, the mechanical withdrawal threshold began to increase in group I 12 hours after operation, and the spinal expression of IL-1β and TNF-α decreased（P＜0. 05）. Conclusion: The spinal IGF-1 could exacerbate incisional pain and spinal inflammation in rats.

Objective To investigate the effects of dexmedetomidine on agitation, extubation reaction and hemodynamics in children after tonsillectomy.Method Totally 103 children who underwent tonsillectomy from December 2019 to December 2020 were randomly divided into control group（n=52） and observation group（n=51）.The observation group was continuously pumped with 0.4 μg/（kg·h） dexmedetomidine; The control group was continuously pumped with equal volume of 0.9% normal saline.The clinical indexes（eye opening time, choking cough score, extubation time）,the classification of agitation score in awakening period, hemodynamic indexes [heart rate（HR）,blood oxygen saturation（SpO 2 ）,mean arterial pressure（MAP）] and the incidence of adverse reactions were observed and compared between the two groups.Result The eye opening time and cough score in the observation group were lower than those in the control group [（9.45±1.67）vs.（20.13±2.36）、（1.35±0.21）vs.（2.42±0.37）]（P＜0.05）; The restlessness score of the observation group was better than that of the control group（P＜0.05）; At T4 and T5,the levels of map and HR in the observation group were lower than those in the control group, and the level of SpO 2 was higher than those in the control group（P＜0.05）; The incidence of adverse reactions in the observation group（1.96%） was lower than that in the control group（13.46%）（P＜0.05）.Conclusion Dexmedetomidine can effectively improve the extubation reaction and agitation in recovery period after tonsillectomy in children, promote hemodynamic stability and high safety.

Objective:To study the role and molecular mechanism of exosome microRNA (miR)-1278 derived from hypoxic lung cancer cells in regulating M2 polarization of macrophages.Methods:The culture supernatants of normal and hypoxic lung cancer cells were collected, and the exosomes were separated by differential centrifugation, observed and counted by transmission electron microscope, and the expression level of exosomal miR-1278 was detected by real-time quantitative polymerase chain reaction (PCR). Collected exosomes (10 μg) were used to treat CD14+ monocytes (mainly macrophages) to induce cell M2 differentiation, and the differentiation extent was evaluated by flow cytometry. MiR-1278 mimic, miR-1278 inhibitor and their negative controls were transfected into CD14+ monocytes to evaluate the role of miR-1278 in the differentiation of M2 macrophages. The secretion of interleukin-10 (IL-10), chemokine (C-C motif) ligand-18 (CCL-18) and vascular endothelial growth factor-A (VEGF-A) were detected by enzyme linked immunosorbent assay (ELISA). The target gene of miR-1278 was predicted, screened and verified, and the regulatory effect of miR-1278 on the target gene and its downstream pathway was investigated. The exosomes from normoxic and hypoxic CL1-5 lung cancer cells were treated with the pathway inhibitor WP1066 or miR-1278 inhibitor, respectively. The proportion of M2 macrophages was detected, and the effects of macrophage conditioned medium on lung cancer cell migration and endothelial cell angiogenesis were studied. Student′s t test was used to analyze the differences between the two groups, and analysis of variance was used to compare the data differences between multiple groups. Results:Compared with normoxic lung cancer cells, the number of exosomes secreted by hypoxic lung cancer cells were increased significantly (upregulated 0.8-1.5 folds, P<0.05). Overexpression and inhibition of miR-1278 promoted normoxic (12.06% vs. 38.21%, P<0.05) and inhibited (30.55% vs. 9.53%, P<0.05) hypoxic M2 polarization of macrophages, respectively, accompanied by changes in the secretion of IL-10, CCL-18 and VEGF-A. MiR-1278 targeted and inhibited the expression of phosphatase SHP-1 (about 70%, P<0.05) and promoted activation of the signal transducer and activators of transcription 3 (STAT3) pathway. WP1066 (STAT3 inhibitor) treatment could inhibit M2 polarization induced by exosomes derived from hypoxic lung cancer cells. Both WP1066 and miR-1278 inhibitor can inhibit tumor cell migration and endothelial cell angiogenesis induced by hypoxic lung cancer exosome-generated conditioned medium in macrophages. Conclusion:MiR-1278 carried by hypoxic lung cancer cell exosomes can target and inhibit the expression of SHP-1 and activate the STAT3 pathway, so as to induce M2 polarization of macrophages, build an immunosuppressive microenvironment, and promote tumor migration and angiogenesis.

目的 探究胸椎旁神经阻滞(TPVB)麻醉对乳腺癌(BC)改良根治术患者术后镇痛效果及免疫功能的影响.方法 选取2019年2月至2021年2月该院收治的110例接受BC改良根治术患者,根据患者意向分为对照组(n=55)和试验组(n=55).对照组采用全身麻醉,试验组在对照组基础上联合TPVB麻醉.比较两组患者手术前后血流动力学指标水平、免疫功能指标水平,以及术后镇痛效果,观察并记录两组不良反应发生情况.结果 术后2 h,试验组平均动脉压、舒张压、收缩压均高于对照组,差异均有统计学意义(t=4.721、6.702、11.908,P<0.05);试验组CD4+、CD4+CD25+T水平变化幅度小于对照组,差异均有统计学意义(t=4.606、6.513,P<0.05);两组术后12、24 h疼痛视觉模拟评分(VAS)均低于苏醒期即刻,差异均有统计学意义(F=39.675、38.321,P<0.05),且试验组苏醒期即刻、术后12 h VAS均低于对照组,差异均有统计学意义(t=10.054、12.738,P<0.05).两组不良反应总发生率比较,差异无统计学意义(χ2=1.886,P>0.05).结论 TPVB麻醉对BC改良根治术应用效果良好,能改善患者免疫功能,具有较好术后镇痛效果,临床疗效良好,安全性佳.

Objective:To identify the differentially expressed genes, construct circular RNA-microRNA-messenger RNA (cirRNA-miRNA-mRNA) regulatory network, and detect the differentially expressed genes with the clinical samples from patients with polycystic ovary syndrome (PCOS) for further investigating of the mechanisms of pathogenesis and providing novel biomarkers for PCOS, to further explore the pathogenesis of PCOS and provide therapeutic targets.Methods:The software 'R' was used to analyze the data from gene expression omnibus (GEO). The differentially expressed genes (mRNA, miRNA and circRNA) were identified, and the mRNA-miRNA-cirRNA regulatory network was predicted by CircInteractome and Starbase database. The retrospective study was performed based on the PCOS patients in the Department of Reproductive Medicine, the Second Affiliated Hospital of Zhengzhou University during January to December in 2018. The cumulus cells were collected from the PCOS (named PCOS group, n=40) and healthy women (named control group, n=20). Reverse transcription real time quantitative PCR (RT-qPCR) was performed to further detect and verify the differentially expressed genes and the network. Results:Analysis from GEO database identified the differentially expressed genes including 278 mRNAs, 23 miRNAs and 2402 circRNAs in PCOS group compared with non-PCOS group ( P<0.05 and |log 2FC|>0.8); 256 mRNA-miRNA-circRNA regulatory networks were established with the differentially expressed genes including 13 mRNAs, 2 miRNAs and 40 circRNAs from the database analysis. The verification with the clinical samples finally revealed the regulatory networks of mRNA pregnancy-associated plasma protein A (PAPPA)-miRNA (hsa-miR-127-3p)-circRNA (hsa_circ_0086809/hsa_circ_0063556) were associated with PCOS ( P=0.004, P=0.002, P=0.014, P=0.003). Conclusion:The expressions of mRNA (PAPPA), miRNA (hsa-miR-127-3p) and circRNA (hsa_circ_0086809/hsa_circ_0063556) in the cumulus cells and their regulatory networks were associated with PCOS.

Objective:To evaluate the effect of propofol on proliferation, invasion and migration of human melanoma cells and role of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2)/matrix metalloproteinase (MMP) signaling pathway.Methods:SKMEL-5 cells were cultured in vitro and divided into 4 groups ( n=36 each) using the random number table method: control group (group C), propofol group (group P), COX-2 overexpression group (group COX-2), and COX-2 overexpression plus propofol group (group COX-2+ P). Propofol at the final concentration of 60 μmol/L was added in group P. The COX-2 overexpression plasmid pcDNA3.1-COX-2 was transfected into SKMEL-5 cells in group COX-2 and group COX-2+ P, and propofol at the final concentration of 60 μmol/L was added in group COX-2+ P.After incubation for 48 h, the cell proliferation rate was determined by CCK-8 method, the cell invasion and migration ability was determined by Transwell assay, the expression of COX-2 in cells was detected by Western blot, the expression of COX-2 mRNA in cells was detected by quantitative real-time polymerase chain reaction, and the concentrations of serum PGE2, MMP-2 and MMP-9 were determined by enzyme-linked immunosorbent assay. Results:Compared with group C, the cell proliferation rate was significantly decreased, the number of cell invasion and migration was decreased, the expression of COX-2 protein and mRNA was down-regulated, and the concentrations of PGE2, MMP-2 and MMP-9 in the supernatant were decreased in group P, and the cell proliferation rate was significantly increased, and the number of cell invasion and migration was increased, the expression of COX-2 protein and mRNA was up-regulated, and the concentrations of PGE2, MMP-2 and MMP-9 in the supernatant were increased in group COX-2 ( P<0.05). Compared with group P, the cell proliferation rate was significantly increased, and the number of cell invasion and migration was increased, the expression of COX-2 protein and mRNA was up-regulated, and the concentrations of PGE2, MMP-2 and MMP-9 in the supernatant were increased in group COX-2+ P ( P<0.05). Conclusions:Propofol can inhibit the proliferation, invasion and migration of human melanoma cells, and the mechanism may be related to inhibition of the COX-2/PGE2/MMP signaling pathway.

Objective:To evaluate the efficacy of remimazolam combined with alfentanil for gastroscopy in frail elderly patients.Methods:Sixty American Society of Anesthesiologists physical status Ⅱ or Ⅲ elderly patients, aged 65-85 yr, with body mass index of 18-30 kg/m 2, of Clinical Frailty Scale score≥5, scheduled for elective painless gastroscopy, were divided into 2 groups ( n=30 each) using a random number table method: remimazolam combined with alfentanil group (group R) and propofol combined with remifentanil group (group P). A combination of alfentanil 10 μg/kg and remimazolam 0.2 mg/kg was intravenously injected until loss of consciousness in group R. Remifentanil 0.5 μg/kg combined with propofol 1.0-2.0 mg/kg was intravenously injected until loss of consciousness in group P. According to the intraoperative conditions, 1/4 of the initial dose of remimazolam was intravenously injected in group R, and 1/4 of the initial dose of propofol was intravenously injected in group P. The time for gastroscopy, requirement for additional remimazolam or propofol, onset time of anesthesia, emergence time and time of post-anesthesia care unit stay were recorded.Physician′s satisfaction scores, patient′s satisfaction scores and Verbal Pain Scale scores were recorded.The occurrence of injection pain, respiratory depression, bradycardia, hypotension and nausea and vomit was recorded. Results:There was no significant difference in the requirement for additional remimazolam or propofol, onset time of anesthesia, time for gastroscopy, physician′s satisfaction scores, and patient′s satisfaction scores, Verbal Pain Scale scores and incidence of nausea and vomit between two groups ( P>0.05). Compared with P group, the emergence time and time of post-anesthesia care unit stay were significantly shortened, and the incidence of injection pain (0 vs.33%), respiratory depression (0 vs.20%), hypotension (3% vs.23%) and bradycardia (3% vs.23%) was decreased in R group ( P<0.05). Conclusions:Remimazolam combined with alfentanil is safe and effective, with rapid recovery from anesthesia, and provides better efficacy than the combination of propofol and remifentanil when used for gastroscopy in frail elderly patients.

目的 比较G-series系列培养基(Vitrolife)与Quinns advantageTM序贯培养基(SAGE)对辅助生殖技术中胚胎体外发育和妊娠结局的影响.方法 回顾性分析2015年1月至2020年9月于郑州大学第二附属医院生殖医学中心接受体外受精/卵胞浆内单精子注射-胚胎移植(in vitro fertilization/intracytoplasmic sperm injection-embryo transfer,IVF/ICSI-ET)患者的临床资料,按照年龄/体质量指数/获卵数/降调方案等筛选纳入人群,根据培养基及受精方式不同进行分组:采用IVF周期的分为Quinn's组(289例)和Vitrolife组(99例),采用ICSI周期的分为Quinn'8组(161例)和Vitrolife组(60例).比较同一受精方式两种不同培养基2PN受精率、2PN卵裂率、D3优质胚胎率、囊胚形成率、总优质胚胎率、临床妊娠率、胚胎植入率、早期流产率等的差异.结果 不论IVF或ICSI周期,vitrolife组的总优质胚胎率(IVF:60.62％ vs 55.19％;ICSI:57.07％ vs 49.13％)、囊胚形成率(IVF:46.15％ vs 34.93％;ICSI:49.17％ vs 32.85％)、临床妊娠率(IVF:68.69％vs 55.36％;ICSI:68.33％ vs 49.69％)均显著高于Quinn's组,差异均有统计学意义(P＜0.05).Logistics回归显示,不同培养基和移植胚胎数是影响临床妊娠的因素.结论 与Quinn's advantageTM序贯培养基相比,使用G-series系列培养基进行体外培养,得到的优质胚胎更多,临床妊娠率也明显提高.

Objective:To investigate the effect of orexin-A (OA) on cognitive function of neonatal rats after brain injury caused by hyperoxia and related molecular mechanisms.Methods:STotally, 63 clean Sprague-Dawley rats with body weight of 40 to 50 g were randomly divided into three groups according to the random number table method: hyperoxic group (HO group, n=21), given high concentration of oxygen (>80%) and intraperitoneal injection of 0.5 ml phosphate buffer saline (PBS) for 7 consecutive days; OA group [hyperoxia+ intraperitoneal injection of 30 μg/kg OA (diluted with PBS to 0.5 ml) for 7 consecutive days, n=21]; control group (group C, n=21) given air inhalation and intraperitoneal injection of 0.5 ml PBS for consecutive 7 days. Morris water maze test was performed on the first day after the successful establishment of the model. After 6 consecutive days, the wet/dry weight ratio (W/D) of the hippocampus was determined. The mRNA expression levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and cold-induced RNA binding protein (CIRP) in the hippocampus were detected by Real-time polymerase chain reaction (PCR). The protein expression of IL-1β, TNF-α and CIRP in the hippocampus was detected by Western blotting. The intergroup ratio of behavioral performance indicators was analyzed by repeated measurement design, and the intergroup ratio of other indicators was analyzed by one-way ANOVA. Results:The residence time in the quadrant of the platform [(17.2±4.4), (25.9±3.6) s] and the average swimming speed [(11.69±0.83), (13.92±0.98) cm/s] in HO and OA groups were significantly lower than those in group C ( F=74.600, 283.000, P<0.05). In HO and OA groups, the escape latency [(48.4±8.6), (38.6±8.1), (33.9±7.4), (19.6±5.7), (22.9±5.5), (41.3±6.4), (33.4±5.5), (27.5±4.7), (15.5±4.5), (16.4±4.3) s], W/D [(5.94±0.36), (5.27±0.33)] of the hippocampus, the mRNA expression level of hippocampal CIRP (3.35±0.63, 1.96±0.30), TNF-α (2.89±0.45, 1.34±0.37) and IL-1β (2.66±0.53, 1.73±0.45) mRNA and the expression of proteins [(0.86±0.23), (0.61±0.19), (0.82±0.26), (0.57±0.22), (0.78±0.18), (0.52±0.15) pg/ml] were significantly higher than those in group C ( F=14.200, 15.620, 14.480, 5.273, 30.280, 57.840, 72.590, 66.660, 32.210, 32.710, 23.520, 47.010, P<0.05). The retention time in platform quadrant and average swimming speed in OA group were significantly increased as compared with those in HO group ( F=74.600, 283.000, P<0.05). The escape latency, hippocampal W/D ratio, the expression of hippocampal IL-1β, TNF-α and CIRP mRNA and proteins in OA group were significantly reduced as compared with those in HO group ( F=14.200, 15.620, 14.480, 5.273, 30.280, 57.840, 72.590, 66.660, 32.210, 32.710, 23.520, 47.010, P<0.05). Conclusion:OA may decrease the expression levels of CIRP, TNF-α and IL-1β in the hippocampus to reduce brain damage caused by hyperoxia in neonatal rats.

目的 比较不同年龄段患者接受1次受卵助孕的体外受精-胚胎移植(in vitro fertilization and embryo transfer,IVF-ET)的临床结局.方法 回顾性分析2015年1月至2019年12月在郑州大学第二附属医院生殖医学中心接受1次受卵IVF及冷冻胚胎复苏移植的75例患者的临床资料,根据患者年龄的不同分为4组,<30岁组26例,30～<35岁组14例,35～<40岁组11例,≥40岁组24例.比较4组患者受卵数、优质胚胎数、冷冻胚胎数及复苏周期移植日内膜厚度、移植胚胎数、临床妊娠率、胚胎着床率、流产率等指标.结果 ①4组患者受卵周期的受卵数、优质胚胎数、冷冻胚胎数、复苏周期移植日内膜厚度、移植优质胚胎数、移植胚胎总数及临床妊娠率比较,差异均无统计学意义(P>0.05);②4组患者的胚胎着床率(年龄由低到高分别为67.65％、36.37％、40.00％、64.71％)、流产率(年龄由低到高分别为0、0、33.33％、35.71％)比较,差异有统计学意义(P<0.05).结论 不同年龄段受卵助孕患者的临床妊娠率相近,但35岁以上受卵助孕患者的流产率较高.

目的 对比激动剂方案和拮抗剂方案在多囊卵巢综合征患者中的卵泡输出率.方法 回顾性选取2018年11月至2019年11月我院多囊卵巢综合征患者100例,依据治疗方法分为拮抗剂方案组(50例)和激动剂方案组(50例)2组,统计分析2组患者的促性腺激素(Gn)用药时间、用量、排卵期前卵泡数(PFC)、获卵数、血清黄体生成素(LH)、雌二醇(E2)水平、内膜厚度、周期取消率、受精率、卵裂率、优胚率、卵泡输出率、妊娠结局.结果 拮抗剂方案组患者的Gn用药时间显著短于激动剂方案组(P<0.05),Gn用量显著少于激动剂方案组(P<0.05),血清LH水平显著高于激动剂方案组(P<0.05),血清雌二醇水平显著低于激动剂方案组(P<0.05),周期取消率显著低于激动剂方案组(P<0.05),但2组患者的PFC、获卵数、内膜厚度、受精率、卵裂率、优胚率、卵泡输出率、胚胎种植率、临床妊娠率之间的差异均无统计学意义(P>0.05).结论 激动剂方案和拮抗剂方案在多囊卵巢综合征患者中的卵泡输出率相当.

Objective:To investigate the changes in lung function of brain death (BD)-induced lung injury and to explore the protection and mechanism of inhalation of 2% hydrogen for BD-induced lung injury.Methods:Twenty-one pathogen free adult Wistar rats were randomly assigned into three groups: brain dead group (BD group, n=7), Sham group (S group, n=7) and hydrogen protection group (BDH 2 group, n=7). Brain-death models were established in BD group and BDH 2 group. Rats inhale mixed gas (hydrogen gas 2%, oxygen gas 50%, nitrogen gas 48%) in BDH 2 group while rats inhale mixed gas (oxygen gas 50%, nitrogen gas 50%) in BD and S group. The arterial blood gas analysis was performed at 0, 30, 60, 90 min after conformation of brain-death. The plasma concentrations of interleukin (IL)-8 and tumor necrosis factor-α (TNF-α) and the activity of superoxide dismutase (SOD), malonaldehyde (MDA) and myeloperoxidase (MPO) in the lungs were measured. The lung injury score (LIS) was recorded. The protein expression of intercellular adhesion molecule-1 (ICAM-1) and cysteinyl aspartate-specific protease (Caspase)-3 in lung tissue were measured by immunohistochemistry. Results:Compared with S group, the values of partial pressure of blood oxygen (PaO 2)/concentration of inhaled oxygen (FiO 2, 301.3±31.0, 352.9±29.0), SOD activity [(10.64±1.25), (12.8±2.17) U/mg·prot], the expression of ICAM-1 and Caspase-3 protein (4.76±0.89, 2.89±0.65, 5.92±1.03, 3.01±0.94) were decreased in BD and BDH 2 group, and the differences were statistically significant ( F=27.440, 4.983, 3.732, 4.237, P<0.05); MDA content [(7.52±1.34), (5.99±0.64) nmol/mg·prot], IL-8 [(538±140), (423±64) pg/ml], TNF-α [(796±213), (569±158) pg/ml] and LIS score (9.28±2.25, 4.70±2.10) were increased in BD and BDH 2 group, and the differences were statistically significant ( F=6.053, 10.079, 9.759, 4.021, P<0.05). Compared with the BD group, the values of PaO 2/FiO 2, SOD activity, the expression of ICAM-1 and Caspase-3 protein were increased in the BDH 2 group, and the differences were statistically significant ( F=27.440, 4.983, 3.732, 4.237, P<0.05); MDA content, the concentrations of IL-8 and TNF-α and LIS score were decreased, and the differences were statistically significant ( F=6.053, 10.079, 9.759, 4.021, P<0.05). Conclusion:Inhalation of 2% hydrogen can ameliorate BD-induced lung injury in rats.

目的:探讨p97抑制剂NMS-873对食管鳞状细胞癌Eca109细胞增殖、凋亡、迁移的影响.方法:用不同浓度的NMS-873分别处理Eca109细胞24、48、72 h,采用CCK-8法检测细胞增殖;在此基础上选用1、2、3μmol/L NMS-873分别作用于Eca109细胞48 h,采用AnnexinV-FITC/PI双染法检测细胞凋亡,Transwell迁移实验检测细胞迁移能力的变化,EdU荧光法检测细胞增殖.结果:NMS-873可呈时间和剂量依赖性抑制Eca109细胞增殖;1、2、3μmol/L的NMS-873分别作用于Eca109细胞48 h后,与阴性对照组相比,随着NMS-873浓度的增加,NMS-873组Eca109细胞凋亡率提高,迁移细胞数减少,增殖率降低(P<0.05).结论:NMS-873能有效抑制Eca109细胞增殖,促进细胞凋亡,并降低细胞迁移能力.

目的:探究Smac过表达对食管鳞癌细胞Warburg效应和细胞凋亡的影响.方法:以含Smac过表达载体的慢病毒感染食管鳞癌细胞EC1和Eca109(Smac过表达组),以感染含空载体的慢病毒为阴性对照,以未感染细胞为空白对照.采用Western blot法检测3组细胞Smac蛋白的表达情况.用过氧化氢刺激Smac过表达组和阴性对照组细胞,利用线粒体膜电位检测试剂盒和Annexin V-FITC/PI双染法检测细胞线粒体膜电位和细胞凋亡情况,并用Western blot法检测细胞中凋亡信号通路相关蛋白[细胞色素C(Cyt-C)、Bcl-2、Caspase-3前体]以及Warburg效应关键蛋白[己糖激酶Ⅱ(HK2)和乳酸脱氢酶(LDH)]的表达.结果:EC1和Eca109 Smac过表达组Smac蛋白表达水平升高,提示成功构建了Smac过表达细胞株.经过氧化氢刺激后,与阴性对照组相比,Smac过表达组HK2和LDH表达水平下降,细胞线粒体膜电位降低,细胞凋亡率升高,Cyt-C的表达水平升高,Bcl-2和Caspase-3前体蛋白的表达水平下降(P<0.05).结论:氧化应激刺激下,Smac过表达可抑制食管鳞癌细胞的Warburg效应,促进食管鳞癌细胞凋亡.

Aim:To observe the effects of DZNep on the growth of esophageal squamous cell carcinoma(ESCC) xenograft and mTOR/p70S6K signaling pathway in vivo.Methods:Eca109 cells were injected into 15 nude mice to establish Eca109 cell xenograft mouse model.These 15 mice were randomly allocated into 3 groups.Mice in one group were intraperitoneally injected with 5-FU every other day for 2 weeks,mice in one group were injected with DZNep,and mice in the last group were injected with normal saline as control.Then,Western blot was employed to detect the expressions of EZH2,SAHH,histone methylation related protein,mTOR/p70S6K signal pathway related protein,Caspase-3,and E-cadherin in tumor tissue.And TUNEL was used to detect the cell apoptosis in tumor.Results:The tumor weight was decreased significantly in DZNep group compared with that in control group (P ＜ 0.05).Meanwhile,the expression of Caspase-3 and Ecadherin and the apoptosis rate were increased significantly in tumor tissue of DZNep group(P ＜ 0.05).The expression of PTEN was increased,and the expressions of H3K27me3,mTOR and phosphorylated p70S6K were decreased in DZNep group(P ＜0.05).Conclusion:DZNep could inhibit ESCC xenograft growth and promote ESCC cell apoptosis in vivo,by inhibiting the activation of mTOR/p70S6K signal pathway.

目的:观察MS-275对食管鳞癌KYSE-70细胞存活、细胞周期、凋亡以及迁移的影响,并分析其对PI3K/Akt/mTOR信号通路相关蛋白p-Akt1和p-mTOR的影响.方法:采用qRT-PCR和Western blot检测KYSE-70细胞和正常食管上皮Het-1A细胞中HDAC1 mRNA及蛋白的表达;CCK-8法检测不同浓度(0.25、0.50、1.00、2.00、4.00、8.00μmol/L)MS-275对KYSE-70细胞存活的影响;以0.00、0.25、0.50、1.00和2.00μmol/L MS-275处理KYSE-70细胞,48 h后流式细胞仪检测细胞周期,Annexin V/PI染色检测细胞凋亡,划痕实验检测细胞迁移情况,Western blot检测细胞中Cyclin D1、Cleaved caspase-3、E-cadherin、p-Akt1和p-mTOR蛋白的表达情况.结果:与Het-1A细胞相比,KYSE-70细胞中HDAC1 mRNA和蛋白表达显著增加(P<0.05);MS-275对KYSE-70细胞存活的影响具有时间和剂量依赖性(P<0.05);随着MS-275处理浓度的增加,KYSE-70 G0/G1期细胞增加、S期细胞降低,细胞凋亡率提高,划痕愈合率降低(P<0.05).MS-275可提高Cleaved caspase-3和E-cadherin蛋白的表达,降低Cyclin D1、p-Akt1和p-mTOR蛋白的表达(P<0.05).结论:MS-275可降低KYSE-70细胞存活率,有效抑制细胞迁移,阻滞细胞于G0/G1期,促进细胞凋亡,其作用可能与PI3K/Akt/mTOR信号通路的抑制有关.

Objective To determine whether intrauterine human chorionic gonadotropin (hCG) infusion before embryo transfer impacts the in vitro fertilization-embryo transfer (IVF-ET) pregnancy outcomes.Methods Databases included PubMed,EMBase,ISI Web of Science Cochrane Library,CNKI,Wanfang and VIP were searched for the study of the effect of hCG on pregnancy outcome.The retrieval period was from the date of database construction to July 2017.The literature screening according to the inclusion and exclusion criteria,followed by quality evaluation and data extraction.The RevMan 5.3.5 software was used for data analysis.Results Twelve randomized controlled trials were analyzed.The results showed that there was a significant increase in clinical pregnancy rates (OR=1.24,95％ CI=1.08-1.43) and implantation rate (OR=1.21,95％ CI=1.00-1.46) in intrauterine hCG group compared with control group,but no significant difference in live birth rate (OR=1.05,95％ CI=0.81-1.36) and abortion rate (OR=1.02,95％ CI=0.78-1.34).Subgroup analysis showed that,for the patients who received the intrauterine hCG ≥ 500 IU before cleavage stage embryo transfer,the implantation rate (OR=1.61,95％ CI=1.32-1.97),the clinical pregnancy rates (OR=1.46,95％ CI=1.26-1.70) and the live birth rate (OR=1.52,95％ CI=1.27-1.84) were greater than those in controls.Conclusion The intrauterine perfusion of hCG can improve pregnancy outcomes of patients with embryo transfer.

Aim:To study the effect of β-carotene on the intercellular ROS level and apoptosis of esophageal squamous cell carcinoma EC1 cell.Methods:EC1 cells were treated with different concentrations of β-carotene(30,50,80 μmoL/L) for 48 h. The content of reactive oxygen species(ROS) in EC1 cells was detected by chemiluminescence method. The change of mitochondrial membrane potential was detected by the conversion of JC-1 from red fluorescence to green fluores-cence. ELISA was used to detect the content of cytochrome C(Cyt-C). The expression of Bax,Bcl-2 and Caspase-3 pro-teins was detected by Western blot. Flow cytometry was used to detect the effects of different concentrations of β-carotene on apoptosis of EC1 cells. Results:β-carotene treatment significantly increased ROS content in EC1 cells,decreased mi-tochondrial membrane potential,and promoted release of Cyt-C. The expression of Bax and Caspase-3 proteins was up-reg-ulated and Bcl-2 protein expression was down-regulated. Meanwhile,β-carotene can significantly increase the rate of apop-tosis of EC1 cells. Conclusion:β-carotene can promote the apoptosis of esophageal squamous cell carcinoma by up-regula-ting the expression of intracellular ROS and decreasing the mitochondrial membrane potential.

Aim:To study the effects of fecal microbiota transplantation(FMT) on learning and memory ability and amyloidβ-protein(Aβ) deposition in Alzheimer′s disease mice.Methods:A total of 15 mice were randomly allocated into APP -group, APP+group, and FMT group(n=5).The APP+mice in FMT group were treated with FMT for 4 weeks, while the mice in APP+group and APP-group were enemaed with normal saline.The learning and memory ability of mice was tested by Morris water maze.And then Western blot was employed to detect the expression of senescence related proteins (P21, P53, Sirt1, Sirt2, PCNA) in mice brain tissue, and the change of Aβdeposition in the brain was observed by Congo red staining.Results:Com-pared with the APP-group, the escaping latency of the APP+group was longer, while the times of crossing the platform quad-rant was less and the time of spending in the target quadrant was shorter(P<0.05).After FMT, the escaping latency of the mice was decreased, the times of crossing the platform quadrant and the time of spending in the target quadrant were increased (P<0.05).And the deposition of Aβin brain tissue of FMT group was reduced compared with the APP +group(P<0.05).Addi-tionally, the expressions of P21 and P53 in brain tissue of FMT group reduced, and the expressions of Sirt1, Sirt2 and PCNA in-creased significantly(P<0.05).Conclusion:FMT could improve the learning and memory ability of Alzheimer′s disease mice by slowing down its aging and reducing the formation of Aβdeposition in brain.

目的 探讨单腔气管内插管保留自主呼吸的静脉全身麻醉下小儿胸腔镜手术的可行性及安全性.方法 选取郑州大学第一附属医院胸外科2012年2～ 12月采用单腔气管内插管保留自主呼吸行静脉全身麻醉下胸腔镜手术治疗的14例患儿作为试验组,男9例、女5例,年龄4～9岁;选取2010年5月至2011年10月采用常规双腔气管内插管全身麻醉下胸腔镜手术治疗的20例小儿患者作为对照组,男13例、女7例,年龄3～10岁.比较两组手术时间、术中失血量、术毕至拔管时间、住院费用、住院时间及术后并发症发生率等指标.结果 两组患儿手术均顺利,无术中术后死亡.试验组和对照组患儿平均手术时间[(85.7±16.9) min vs.(83.5±16.5)]及术中失血量[(90.0±55.0) ml vs.(85.0±50.0) ml]差异无统计学意义(P＞0.05).试验组的术毕至拔管时间[(0.5±0.1) minvs.(8.3± 1.4) min]、住院费用[(24.3±4.7)千元vs.(27.8±5.3)千元]、住院时间[(6.6±0.9)d vs.(12.7±3.2)d]、术后并发症发生率(7.1％vs.25.0％)都显著短或少于对照组(P＜0.05).结论 单腔气管内插管保留自主呼吸静脉全身麻醉下小儿胸腔镜手术治疗小儿胸部疾病具有一定的安全性及可行性.