The regeneration of periodontal bone is hindered by unresolved inflammation, insufficient angiogenesis, and compromised osteogenesis, conditions that are rarely addressed simultaneously by existing scaffold designs. Conventional hydrogel-based constructs often lack the mechanical robustness required for maxillofacial defects, while many synthetic scaffolds fail to replicate the multiscale pore organization essential for functional regeneration. Here, we present a dual-fabrication strategy for constructing hierarchically porous scaffolds via digital light processing (DLP)-based 3D printing of a porogen-containing resin. This hybrid approach achieves well-defined macropores for load-bearing stability and vascular infiltration, together with interconnected hierarchical micro-nanoporous that promote nutrient transport and cell-matrix interactions. To endow the scaffold with biological functionality, it was modified with polydopamine (PDA) for efficient immobilization and sustained release of erythropoietin (EPO). The resulting scaffolds (DMPS-PDA-EPO) simultaneously regulated inflammation by promoting M2 macrophage polarization, enhanced angiogenesis through endothelial migration and tube formation, and stimulated osteogenic differentiation of human periodontal ligament stem cells under pro-inflammatory conditions. In vivo implantation in rat periodontal defect models resulted in markedly enhanced bone regeneration, with bone volume fraction and bone mineral density increasing by 161.9% and 167%, respectively, at 8 weeks compared with controls. By integrating structural precision, mechanical robustness, and immuno-angiogenic bioactivity, this EPO-functionalized hierarchical scaffold represents a clinically translatable strategy for the treatment of complex periodontal defects.

BACKGROUND:Schisandrol A (SA), the primary active component of Schisandra chinensis, exhibits antioxidant properties, safeguarding cells from oxidative damage. However, its effect on wound healing remains elusive, and its low water solubility restricts its utilization efficiency. OBJECTIVE:This study aimed to explore the impact of SA on HaCaT cell proliferation and migration through in vitro experiments, thereby providing deeper insights into its role in skin wound healing. RESEARCH DESIGN:In vitro cell experiments and in vivo mouse wound model experiments were employed to investigate the effects of SA on cells and wound healing. METHODS:1. In vitro Experiments: HaCaT cells were used to observe the influence of SA on their proliferation and migration. 2. RNA Sequencing: RNA sequencing was utilized to determine the molecular mechanism underlying the healing effect of SA, specifically the regulation of miR-21/WNT5A expression by FTO. 3. Animal Experiments: In a mouse wound model, PF-127 hydrogel was used as a dressing for the delivery of exosomes and SA. The wounds of the group treated with SA alone were compared with those of the group treated with SA-carrying exosomes. RESULTS:1. SA enhanced HaCaT cell migration and proliferation. 2. RNA sequencing indicated that the healing effect of SA was mediated by the regulation of miR-21/WNT5A expression via FTO. 3. In the mouse wound model, the group treated with SA-carrying exosomes demonstrated enhanced wound-healing speed and efficacy than in the group treated with SA alone. These results were further confirmed by histopathological and biochemical analyses. CONCLUSION:Exo-SA modulates the FTO/miR-21/WNT5A axis to promote skin healing.

Tongue squamous cell carcinoma (TSCC) is a prevalent malignancy that afflicts the head and neck area and presents a high incidence of metastasis and invasion. Accurate diagnosis and effective treatment are essential for enhancing the quality of life and the survival rates of TSCC patients. The current treatment modalities for TSCC frequently suffer from a lack of specificity and efficacy. Nanoparticles with diagnostic and photothermal therapeutic properties may offer a new approach for the targeted therapy of TSCC. However, inadequate accumulation of photosensitizers at the tumor site diminishes the efficacy of photothermal therapy (PTT). This study modified gold nanodots (AuNDs) with the TSCC-targeting peptide HN-1 to improve the selectivity and therapeutic effects of PTT. The Au-HN-1 nanosystem effectively targeted the TSCC cells and was rapidly delivered to the tumor tissues compared to the AuNDs. The enhanced accumulation of photosensitizing agents at tumor sites achieved significant PTT effects in a mouse model of TSCC. Moreover, owing to its stable long-term fluorescence and high X-ray attenuation coefficient, the Au-HN-1 nanosystem can be used for fluorescence and computed tomography imaging of TSCC, rendering it useful for early tumor detection and accurate delineation of surgical margins. In conclusion, Au-HN-1 represents a promising nanomedicine for imaging-based diagnosis and targeted PTT of TSCC.

Aim or purpose: To address ferroptosis-driven orofacial flap necrosis, this project developed a multifunctional hydrogel combining wet adhesion, antibacterial properties, and iron-scavenging capabilities. Materials and methods: HD hydrogels were synthesized through EDC/NHS-mediated crosslinking of hyaluronic acid (HA) and dopamine (DA), demonstrating robust wet adhesion and mechanical flexibility. Functionalization with tannic acid (TA) further enhanced adhesion strength while introducing iron-chelating and antimicrobial capabilities. Mechanical properties were analyzed using Tensile tests, Rheometry, and Adhesion measurements. Iron adsorption capacity was determined by SEM and EDS. Antibacterial performance assessed through inhibition zone and live/dead staining against S. aureus, E. Coli and S. mutans. A BALB/c mouse ischemic skin flap model (1×2.5 cm, n=5/group) was used to evaluate flap survival (postoperative days 3, 5, 7), histological repair (HE/Masson staining), and iron accumulation (Prussian blue staining), with all animal procedures conducted in compliance with ethical guidelines for experimental research. Results: HD-TA hydrogel exhibited strong wet adhesion (61.2kPa), efficient iron chelation (73.6%), and broad antimicrobial efficacy (87.2–94.3% inhibition).In vivo treatment significantly improved flap survival rates (93.3 ± 3.8% vs. 58.7 ± 4.5% in controls, p < 0.001) with improved histological regeneration, including increased neovascularization and collagen deposition along with reduced inflammatory infiltration. Conclusions: This TA-modified hydrogel establishes a dual-action therapy targeting ferroptosis and bacterial infection for enhanced flap recovery.

Periodontitis is an increasingly prevalent oral inflammatory disease, whose treatment faces potent challenges because of recurrent bacterial infections, excessive reactive oxygen species (ROS) production, as well as unrelenting inflammation-induced alveolar bone resorption during the pathological process. Herein, we present the rational design and construction of a multifunctional hydrogel platform composed of the quaternized chitosan/oxidized dextran (QCS/OD) hydrogel component with high bactericidal activity and immunomodulatory carbonized polymer dots from resveratrol (RSV-CPDs) for periodontitis treatment. Such RSV-CPDs@QCS/OD (RCQD) hydrogels exhibit characteristics including shape adaptability, self-healing properties, and pH-responsibility, enabling it to adapt to irregularly shaped periodontal tissues, degrade gradually in the mildly acidic microenvironment of periodontitis, and release immunomodulatory RSV-CPDs in a controlled manner. Significantly, RCQD can effectively suppress the proliferation of bacteria as well as alleviate oxidative stress, and relieve inflammation via activating the Nrf2/NF-kappa B signaling pathway, ultimately fostering a favorable environment for the regeneration of both the soft and hard periodontal tissue. Overall, this study highlights the great potential of RCQD for treating periodontitis by addressing the practical needs in tissue regeneration.

Topotactic transformation is an emerging strategy for synthesizing materials with exotic functional properties. In this report, instead of producing new crystals with related structures, we exploited the topotactic transformation phenomenon to spontaneously produce compositionally diverse nanostructures on the transforming substrate. The surface of magnetite nanoparticles (Fe3O4 NPs) is topotactically transformed into maghemite (γ-Fe2O3). Benefiting from such oxidation susceptibility of ultrasmall Fe3O4 NPs, we achieved spontaneous growth of metals (Ag, Au, Pt, and Pd), a non-metal (Se), and a metal oxide (MnO2) based nanostructures onto the surface of Fe3O4. No spontaneous growth of nanostructures was observed when the oxidized Fe3O4 NPs were tested, likely due to the loss of the Fe2+-associated mobile electrons. The obtained nanostructures displayed appreciable antioxidant activities, which we utilized to effectively treat inflammation in the intestines. It is anticipated that this synthetic route, based on topotactic transformation, represents a significant advancement in synthesizing various chemically diverse hetero-nanostructures.

OBJECTIVE:This report includes the description of a case of parotid Castleman's disease (CD), along with a review of relevant literature from the last 30 years. The clinical features, pathological classification, clinical diagnosis, treatment modalities, and prognosis of CD have been discussed with the aim of improving clinicians' understanding of its diagnosis and treatment. METHODS:The clinical data of one patient with parotid CD were collected, and the related literature was reviewed. RESULTS:The patient was diagnosed with CD based on pathological and imaging findings. There are two clinical subtypes of CD - unicentric CD (UCD) and multicentric CD (MCD). UCD is further classified into the hyaline-vascular (HV) and plasma cell (PC) types. The patient underwent parotid gland tumor resection under general anesthesia, and has recovered well and remains under regular follow-up. CONCLUSION:CD is a rare, benign lymphoproliferative disorder with diverse pathological subtypes. Surgical resection remains the primary treatment. For patients with suboptimal surgical outcomes or contraindications, immunotherapy or chemotherapy are necessary adjunctive treatments. The prognosis of CD is influenced by various factors, including the pathological subtype, but is generally favorable.

Hydrogel films have emerged as a significant focus in the field of food preservation, particularly those with multifunctional properties that show considerable promise in both protecting food and monitoring its condition. This research details the development of an antioxidant nanozyme (CuLyz) designed to preserve freshness, achieved by chelating copper ions with lysozyme (Lyz). Subsequently, a multifunctional hydrogel film (PVA/TA/CuLyz) was created, incorporating polyvinyl alcohol (PVA), tannic acid (TA), and CuLyz. The resulting hydrogel film exhibits remarkable toughness, characterized by a tensile strength of 3.49 MPa and an elongation at break of 615 %, effectively addressing challenges associated with impacts during packaging and transport. Furthermore, this film demonstrates exceptional attributes, including UV blocking, antioxidant effects, oxygen resistance, antimicrobial properties, and the ability to preserve freshness. When utilized as packaging for strawberries, it can extend their shelf life by up to 6 days and showed excellent antimicrobial effect against E. coli (96 %) and S. aureus (95 %). In addition, the film integrates multifunctional sensors and enables intelligent decay detection through machine learning-based visual recognition. This study describes an innovative approach aimed at advancing hydrogel film technology for fruit packaging freshness.

Nanodynamic therapy (NDT), as an emerging cancer treatment strategy, achieves specific killing of tumor cells by using nanomaterials to generate reactive oxygen species (ROS) under the activation of external energies (e.g. light, acoustic, thermal, and electrical, etc.). This article systematically reviews the classification of NDT and its mechanism of action, including photodynamic therapy (PDT), sonodynamic therapy (SDT), thermodynamic therapy (TDT), etc. and explores in detail the multiple pathways of tumor cell death (e.g. apoptosis, iron-death, copper-death, and cell-cycle blockade) induced by NDT. In addition, the article focuses on analyzing the targeting strategies (e.g. targeting peptides, nucleic acids, folate receptors, and mitochondrial targeting) and drug delivery systems (e.g. exosomes, liposomes, and nano-metal-organic frameworks) of NDT to enhance the precision and efficiency of the treatment. By combining chemotherapy, immunotherapy and bacterial therapy, NDT further overcomes tumor microenvironmental limitations and enhances therapeutic efficacy. Clinical studies have demonstrated the potential of NDT in the treatment of brainstem glioma and prostate cancer. Future studies should focus on optimizing sensitizer design, improving the tumor hypoxic microenvironment, and developing multifunctional nanoplatforms to promote the clinical translation of NDT.

Owing to high rates of antibiotic resistance, the elimination of periodontal plaque biofilms has become a significant clinical challenge. In this context, metal-organic framework (MOF)-based photodynamic therapy (PDT) has emerged as a novel antimicrobial treatment option. However, this therapeutic strategy suffers from drawbacks such as the insufficient generation of reactive oxygen species and the lack of targeted biofilm clearance, which greatly hinder its clinical application. Here, a multifunctional MOF-based nanocomposite (ICG@Uio-66-UBI) was developed by modifying MOFs (Uio-66-NH2) with an antimicrobial peptide (UBI29-41) to enhance PDT efficiency. Our findings showed that the UBI29-41 targets EPS and selectively binds to lipopolysaccharide (LPS) on bacterial surfaces via electrostatic interactions, enabling precise delivery of ICG-generated ROS under 808-nm near-infrared irradiation, which disrupts bacterial membranes and inhibits biofilm formation. Subsequently, UBI29-41 blocks LPS-TLR4 binding, suppressing NF-κB signaling and reducing pro-inflammatory cytokine production. Furthermore, the nanocomposite significantly downregulates the LuxS/AI-2 quorum sensing (QS) system, reducing AI-2 synthesis and virulence gene expression, thereby inhibiting biofilm formation. In vivo studies confirmed the platform's efficacy in inhibiting biofilm formation and preventing collagen degradation in gingival tissue. By synergistically combining targeted antimicrobial action, anti-inflammatory effects, and QS modulation, ICG@Uio-66-UBI represents a breakthrough in precision periodontal therapy, offering a potent solution for biofilm-associated infections.

Small molecule electrode materials with superb redox activity have significant applied implications for K-ion storage, but they face significant challenges like high solubility in electrolytes and low conductivity, limiting their capacity, rate, and cycling stability. Herein, a series of Ni-bis(dithiolene) (NiS4)-based small molecules are designed with control of various redox-active substitutional groups for K-ion batteries anode materials. It is identified that bis[1,2-di(pyridine-4-yl) ethylene-1,2-dithiolate] nickel Ni[C2S2Py2]2 demonstrates a high reversible specific capacity (399 mAh g-1 at 0.03 A g-1) with an impressive rate capability and an exceptional cycling stability (over 99% capacity retention after 1600 cycles). Its extraordinary performance is attributed to the synergy between the NiS4 unit and pyridine group, providing abundant K⁺ storage sites, impressive conductivity, and low solubility. The comprehensive characterizations and theoretical simulation confirm the multistep K⁺ storage mechanism in Ni[C2S2Py2]2, enabling fast charge transfer and excellent rate performance. This work offers new perspectives in building solubility-limited and conductive small molecule electrode materials with high redox activity for non-aqueous rechargeable batteries.

Conductive hydrogels have garnered significant attention in the realm of future flexible electronic devices due to their properties such as flexibility,electrical conductivity,frost resistance,and biocompatibility.However,the integration of numerous functional applications in the biomedical field still presents notable challenges.In this research,a rigid hard-structured network was formed by cross-linking gallic acid grafted chitosan(CS-GA)and tannic acid(TA)with poly(vinyl alcohol)(PVA)through physical freezing.The noncovalent hydrogen bonding during the freezing and thawing process facilitated the formation of microcrystalline domains in the amorphous hydrogel network system.Functional proteins from eggshell membrane were cross-linked with tetra-armed poly(ethylene glycol)maleimide(4am-PEG-MAL)via thiol-olefin click chemistry,and lysozyme was incorporated into the network as an antibacterial component through the nucleophilic substitution reaction.These chemical cross-linking methods resulted in a soft-structured network that enhanced the mechanical properties of the hydrogel(maximum stress of 2.15 MPa and elongation of 605％).The use of ionic liquids/ethylene glycol/water(ILs/EG/H2O)ternary solvents instead of a single solvent not only provided frost resistance but also imparted excellent electrical conductivity to the hydrogels(0.37±0.04 S/m).Notably,the organohydrogel showed good antimicrobial properties and biocompatibility and was effective in providing emergency cooling after fireworks burns and promoting wet healing of broken skin to minimize scarring.In the biomedical field,this multifunctional hydrogel can serve as a flexible wearable device to monitor the movement amplitude of wounds in real-time,offering a novel approach to deep learning-assisted wound healing.The multifunctional nature of this organohydrogel as a flexible wearable device in the biological field presents promising applications.

Oral squamous cell carcinoma (OSCC) is a tumor characterized by cellular redox imbalance, rendering it particularly sensitive to ferroptosis treatment. However, traditional ferroptosis inducers have a few drawbacks. In this study, ultrasmall AuMn nanoclusters (AMNCs) with a bovine serum albumin (BSA) ligand were synthesized and encapsulated in natural killer (NK) cell-derived exosomes to form an Exo-AMNCs composite for targeted ferroptosis therapy of OSCC. Unlike previously reported alloyed metal nanoclusters, not only do AMNCs react with intracellular H2O2 to produce reactive oxygen species (ROS) and induce ferroptosis but also the BSA ligand improves biocompatibility and water solubility. These properties render AMNCs ideal for fluorescence imaging in vivo. When combined with NK cell exosomes, the Exo-AMNCs composite exhibited strong targeted imaging and therapeutic effects on OSCC. Further investigation into the mechanistic details demonstrated that Exo-AMNCs downregulate the overexpression of fat mass and obesity-associated (FTO) in OSCC and regulate the key ferroptosis-related protein glutathione peroxidase 4 (GPX4).

Implant dentures have become the main method for the treatment of dentition defects or complete edentulism. However, due to the lack of periodontal ligament and periodontal ligament proprioceptors, implant dentures have very limited cushioning and sensing capabilities and are prone to occlusal overload. As a risk factor for peri-implantitis, occlusal overload seriously threatens the stability and success rate of implant dentures. This paper reviews the occlusal overload of implant dentures, the causal relationship between occlusal overload and plaque biofilms in peri-implantitis, the mechanism by which occlusal overload promotes peri-implantitis, and the effect of reasonable clinical occlusal adjustment on healing. This review shows that occlusal overload is closely related to the occurrence of peri-implantitis. Occlusal overload can promote the process of peri-implantitis by increasing the release of inflammatory factors and mechanical transduction mechanisms. The intervention of the patients’ bad bite habits and occlusal adjustment can promote the healing of peri-implantitis. At present, there is no uniform standard ideal experimental model for occlusal overload. The phenomenon and mechanism of bone resorption around the implant caused by overload force still need further observation and research, which will help determine the intensity, direction and timing of occlusal loading to guide clinical occlusal adjustment.

Rationale: Traditional free gingival graft (FGG) technique is usually used for patients with insufficient peri-implant keratinized mucosa. However, this technique often requires a second surgical area which increases the pain as well as the risk of infection in patients. Xenogeneic collagen matrix (XCM) membrane technique can obtain good results for keratinized mucosa increment. Patient concerns: The patient was a 66-year-old healthy female with loss of left mandibular first molar and second molar (FDI #36, #37) for 5 years. Two implants were placed submucosally for 3 months with no interference, while a stage II surgery was needed. Diagnosis: Probing depth measurements suggested that the mesial, medial, and distal widths of buccal keratinized mucosa within the edentulous area were 0.5, 0.5, and 1 mm, respectively, which were insufficient to maintain the health of peri-implant tissues. Interventions: Keratinized mucosa augmentation guided by XCM membranes was performed to increase the inadequate buccal keratinized mucosa. Outcomes: After 2 months of healing, the widths of mesial, medial, and distal buccal keratinized mucosa were 4, 3, and 3 mm, respectively, and the thickness of the augmented mucosa was 4 mm. Then a stage II surgery was followed. The patient was satisfied with the outcomes of keratinized mucosa augmentation. Lessons: Keratinized mucosa augmentation guided by double XCM membrane technique can be applied to cases with keratinized mucosa width within 2 mm around implants.

口腔种植体软组织屏障对种植体长期稳定性及软组织美学效果的维持具有重要意义,且软组织屏障的建立受种植体表面形貌的影响.本文就钛表面纳米形貌对上皮细胞的生物学行为研究进展做一综述.

目的 探讨二十二碳六烯酸(DHA)对牙龈卟啉单胞菌(P.g)脂多糖(LPS)诱导巨噬细胞炎症的抑制作用.方法 用CCK-8方法检测DHA对P.g-LPS诱导的小鼠单核/巨噬细胞系RAW264.7细胞的毒性.将P.g-LPS与RAW264.7细胞共培养24 h后,更换含有DHA的培养基培养24 h,实时荧光逆转录定量PCR(qRT-PCR)检测RAW264.7细胞中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)和白介素-6(IL-6)mRNA的表达.酶联免疫吸附实验检测细胞上清液中TNF-α、IL-1β、IL-6的分泌.用DCFH-DA方法检测RAW264.7细胞内活性氧(reactive oxygen species,ROS)的产生.结果 1 mg/L的P.g-LPS对巨噬细胞无毒性作用(P>0.05),浓度≥100μmol/L的DHA对P.g-LPS诱导的RAW264.7细胞有明显毒性作用(P<0.05);P.g-LPS组TNF-α、IL-1β、IL-6 mRNA表达显著高于阴性对照组(P<0.05);25、50、75μmol/L的DHA减少TNF-α、IL-1β、IL-6 mRNA的表达和TNF-α、IL-6的合成(P<0.05);P.g-LPS组ROS产生增多,25、50、75μmol/L的DHA减少ROS的产生(P<0.05),抑制程度呈浓度依赖性.结论 DHA能够有效抑制P.g-LPS诱发的巨噬细胞炎症反应.

二十二碳六烯酸(DHA)属于ω-3多不饱和脂肪酸,主要存在于深海鱼类、海藻和鱼油补充剂中,具有抗炎、抗菌、抗癌等多种生理功能.口腔健康与人们生活质量密切相关,随着人们生活水平的提高,人们对口腔疾病的关注度逐渐提升,DHA可在一定程度上防治牙周、牙体疾病、口腔鳞状细胞癌以及复发性阿弗他口炎等,通过研究DHA与口腔疾病的关系以及DHA治疗口腔疾病的分子机制和临床应用有助于寻找防治口腔疾病的新药物,并为口腔疾病的治疗提供新思路.

目的:研究结扎诱发的大鼠牙周炎模型中炎症的动态变化。方法:选取健康雄性SD大鼠21只,随机分为7组,分别为正常对照组和结扎组A-F（结扎组A-F分别依次结扎1、3、5、7、14、21 d后处死）。除正常对照组各组均选用结扎丝于右侧上颌第一磨牙牙颈部结扎,建立大鼠实验性牙周炎模型,通过观测21 d内大鼠体重变化,探究结扎丝的介入对实验性牙周炎炎症过程的影响,通过软垢指数（DIS）、龈沟出血指数（SDI）及牙齿松动度（TM）的监测,评估结扎21 d内目标牙牙周炎症变化,为了更精确地评估炎症阶段牙槽骨的动态变化,使用CBCT观察牙槽骨吸收情况。结果:结扎组A-F在结扎后3 d体重均下降,第3天体重开始恢复上升。并且与正常对照组相比,结扎组3～21 d的体重增长率比较,差异无统计学意义（P＞0.05）,说明由于结扎丝的介入,大鼠会出现3 d的适应性现象,而在结扎3 d后结扎丝即对大鼠生长发育不会产生影响;结扎侧DIS在结扎后第1、3、5、7天较正常对照侧显著升高（P＜0.05）,且DIS值在前3 d呈上升状态,第5天开始逐渐下降,直至结扎第21天与正常对照组比较,差异无统计学意义（P＞0.05）;结扎侧SDI在第5天明显升高,第7天达到峰值后逐渐下降（P＜0.05）,直至结扎第21天与正常对照组比较,差异无统计学意义（P＞0.05）;结扎侧TM第7天开始出现变化,直至第21天逐渐上升,与正常对照组比较,差异有统计学意义（P＜0.05）;结扎组A、B、C的CEJ-ABC距离与正常对照组比较,差异无统计学意义（P＞0.05）,结扎组D、E、F的CEJ-ABC距离明显高于正常对照组（P＜0.05）,且F组与D、E组对比骨吸收更加显著（P＜0.01）。结论:结扎诱导的大鼠实验性牙周炎在结扎后3 d为牙周炎始发期;结扎的3～7 d处于急性炎症期;14～21 d转为慢性炎症阶段,为慢性牙周炎的炎症进展期。本研究对结扎诱导的大鼠牙周炎发生发展过程进行了更加精细地定义,为后续对牙周炎模型的研究提供了新的方向,为更好更准确地进行临床研究奠定基础。

目的:比较胸锁乳突肌瓣与口腔修复膜在腮腺手术术后面部凹陷畸形修复和Frey综合征预防上的临床效果.方法:收集80例吉林大学口腔医院颌面外二科进行的腮腺良性肿瘤的患者,随机分为皮瓣组、修复膜组和对照组三组.皮瓣组在腮腺手术的基础上用胸锁乳突肌瓣进行修复;修复膜组在腮腺手术的基础上用口腔修复膜进行修复;对照组在腮腺手术后不做任何处置.术后72 h和术后6个月进行随访,并观察术区面部凹陷畸形、Frey综合征和颈肩膀运动障碍发生的差异.结果:皮瓣组比修复膜组和对照组能更好地恢复术区面部凹陷畸形且具有统计学意义(P<0.05);皮瓣组和修复膜组与对照组相比均能有效预防Frey综合征的发生,且皮瓣组具有更好的预防效果(P<0.05);本实验中皮瓣组均未发生副神经损伤.结论:胸锁乳突肌瓣比口腔修复膜具有更好的恢复腮腺良性肿瘤术后面部凹陷畸形和预防Frey综合征的效果,值得推广和应用.