11月1日至3日,四川省关工委名誉主任谢世杰、常务副主任陈官权一行到乐山,就乐山市关心下一代工作进行调研.调研组在深入学校、乡镇实地考察,并听取市关工委工作汇报后,对乐山市关心下一代工作取得的成绩给予肯定.乐山市委常委、市总工会主席、市关工委主任方为加出席汇报会.乐山市关工委执行主任李留根陪同调研并汇报了该市关心下一代工作情况.

<正>不久前,从全国人力资源和社会保障工作会议上传出喜讯:乐山市社会保险局被授予"全国人力资源和社会保障系统2008-2010年度优质服务窗口"称号。这是该局继2007年荣获全国劳动和社会保障系统"集体一等功"

The components of fatty acids in Wenchang chicken were analyzed by gas chromatogrphy-mass spectrometry technology(GC-MS),and the relative content of each fatty acid were calculated.16 kinds of fatty acids were seperated from Wenchang chicken.Among them,7 kinds of fatty acids,including lauric anid,myristic acid,pentadecanoic acid,palmitic acid,margaric,stearic acid and eicosanoic,were saturated fatty acids.The content of those 7 fatty acids were 0.75%,1.23%,0.43%,23.86%,2.04%,11.18%,0.13%,accounts for 39.63% of the total fatty acid.9 kinds of fatty acids,including 11-teteadecenoic acid,9-hexadecenoic acid,9-vaccenic acid,9,12-octadecadienoic acid,6,9,12-octadecatrienoic acid,11-eicosenoic acid,10,13-eicosenoic,7,10,13-eicosatriennoic and 5,8,11,14-arachidonic acid,were unsaturated fatty acids.The contens of 9 kinds of fatty acids were 0.15%,1.79%,25.84%,21.99%,0.48%,0.32%,0.45%,0.61%,8.75%,accounts for 60.37% of the total fatty acid.The polyunsaturates fatty acids accounted for 32.29% of the total fatty acids and the essential fatty acids accounted for 31.23%.The results provided the basis for analysis on fatty acid acid component and evaluation on the nutritive value.

The aim of the present study was to detect single nucleotide polymorphism(SNP) in some areas of IL-1β gene of chicken to find genetic marker influencing on IL-1 content in serum.Two pairs of primers were designed according to the sequence of IL-1β gene of red jungle fowls,and the SNP of Rugao chicken(RG),Wenchang chicken(WC),and Anka chicken(AK) were analysed by PCR-SSCP,meanwhile,the IL-1 content in serum were detected at 56 days with reagent kit by ELISA.The results showed that the IL-1 content in serum of AK was significantly higher than that of RG(P0.01) and WC(P0.001).The mutation were found at four sites in all three breeds,and they were mutation G→A at 476 site,mutation T→C at 482 site,mutation C→T at 1 215 site and mutation T→C at 1 507 site.Then,the correlation between polymorphic sites and content of IL-1 were analysed,and only 1 215 site had relationship with IL-1 content in serum,which showed that chicken with TT genotype had significantly higher IL-1 content in serum than those with CC genotype.This study indicated that the 1 215 polymorphic site could be a genetic marker for IL-1content in serum.

[Objective]The SNPs of IL-1β gene and their correlations with some immune traits were tested. [Method]A pair of primers were designed as the sequence of IL-1 β of red jungle fowls,and the SNPs of intron 1 and 2 of IL-1β gene in Rugao Chicken(RG),Wenchang Chieken(WC),and Anka Chicken(AK) were detected using PCR-SSCP.Meanwhile,concentration of IL-1,H/L value,SRBC andbody filer,ND antibody tiler and AI antibody filer were detected at 56 days of age.Then,the correlation between SNP sites and immune traits were analyzed. [Results]Totally 6 SNP sites were detected at T107G,T130A,C143T,A208C,T241G and T274C.Furthermore,the mutation at 107,208,241 and 274 sites were found association with immune traits (P＜0.05 or P＜0.01),and some genotype combinations significantly affected H/L value and ND antibody tiler (P＜0.05).[Conclusion]The mutation at 241 site may be selected as a new candidate mark in disease resistance breeding because of its better performance in three immune traits than that of wild type chickens.Selecting breeder chickens by genotype combinations had more effect than by single.genotype of SNPs.

The content of Intramuscular Fat (IMF) of Anka chicken, Wenchang chicken and Rugao chicken from 4-12 weeks old were tested in this study. The results showed that Intramuscular fat content in three chicken breeds increased with the age. Herein, the IMF of breast muscle in Anka chicken sharply increased after 8 weeks old and was higher than that of Wenchang and Rugao chicken. But, there was no significant difference between different breeds before 8 weeks old. The IMF of hen is higher than that of cock in all three breeds, but only in Wenchang chickens the significant difference were detected between different sex.

The present study compared some immune traits to evaluate the disease resistance of different chicken breeds.Random samples of 80 chickens from each population of Rugao Chicken (RG),Wenchang Chicken (WC),and Anka Chicken(AK) were partitioned into A and B groups.Each group had an injection of 0.5 mL and 1 mL of 25% suspension of sheep red blood cell (SRBC) at 49 days of age,respectively.The value of heterophil/lymphocyte(H/L) at 41,56,71and 89 days of age,SRBC antibody titer at 56,71and 89 days old,and the antibody of Newcastle disease (ND) and avian influenza(AI) at 30,41,56,71 and 89 days of age were detected,respectively.The results showed as follows:The difference of H/L value existed between different breeds in the same days of age,but it became smaller along with time.H/L value in RG changed less than that of other breeds,however,WC and AK had obvious change from high to low level.The SRBC antibody titer of three breeds got the highest at 56 days,the highest with WC and the lowest AK(P0.05).Moreover,there were significant differences between them.The sensitivities to different dosages of breeds were different except RG.ND antibody titer of three breeds had the change,variable,though the AK's antibody titer was in the mid of RG and WC before the third immunity,it was extremely significantly higher than the other two breeds of chicken after that period (P0.01).There were insignificant difference of AI antibody titer between RG and WC in all test time except 56 day,meanwhile antibody titer of AK was extremely significantly lower than that of the other two breeds in all test time(P0.001).This study showed that the immune traits were different between chicken breeds,and Chinese chicken breeds had the some advantage of outside breeds.

Single nucleotide polymorphisms(SNPs) of Δ9-fatty acid desaturatase gene were detected by PCR-SSCP in this study,and correlation between different genotype and breast muscle fatty acid content was analyzed.The results showed that there was no SNP mutation in exon 6,but two SNPs,T101C and C197T in exon 2 with three genotypes,in which allele A was predominant,its frequency were beyond to 0.81 in three breeds.This site was in Hardy-Weinberg equilibrium in all three chicken breeds(P0.05).Association analysis by least squares test showed that PUFA and EFA contents of the chicken with genotype AB were significantly higher than those of the other two genotypes,AA and BB(P0.05).It revealed that AB genotype had an important impact on intramuscular fat.

Studies on the law of body growth of 0~12-week-old Rugao chicken(RG),Wenchang Chicken(WC),and Anka Chicken(AK),and the nonlinear curves of Logistic model were used to fit the growth model.The result s were as follows.There were significant differences(P0.05) in the body weight between male and female in 2~12-week-old,but the growth and development had the same change.There were great differences between the breeds of chickens,because the growth and development had significant differences in all time among breeds,but there was no significant difference in the relative growth rate on 6-week-old and absolute mass growth per day on 12-week-old between RG and WC,and in the relative growth rate on 10-week-old between RG and AK.All the three breeds of chickens' growth can be demostmted greatly according to Logistic model(R20.99).The predicted body weight was close to ture body weight.

Single nucleotide polymorphisms (SNPs)and effect of polygene pyramiding of 3 candidate genes affecting Rugao chicken intramuscular fat trait,H-FABP,A-FABP and Δ9-FAD,were analyzed by PCR-SSCP technique. Three mutations of A1243G,A1763G and C197T and three kinds of genotypes were detected. As a result,BB genotype of H-FABP and Δ9-FAD gene and AB genotype of A-FABP gene were significantly higher than the other two types in intramuscular fat content by test of minimum least-squares analysis (P0.05). But the pyramiding genotypes,whether belong to two genes or three genes,had not shown the corresponding advantages with the single gene,and obvious genetic rules to follow. It was concluded that pyramid simply each advanced genotype was not the best pyramiding plan. It should be based on the clear and consistent genetic effect of each single candidate marker,especially for complex traits such as intramuscular fat and other meat traits.

随机抽取如皋、文昌和安卡鸡各80只,经新城疫和禽流感免疫后分别于30、41、56、71和89日龄检测抗体水平变化。结果表明,如皋鸡公母抗体水平无明显差异,而文昌鸡和安卡鸡在性别之间差异显著。文昌鸡新城疫抗体在所有检测日龄均低于如皋鸡和安卡鸡,而安卡鸡禽流感抗体在所有检测日龄均极显著低于如皋鸡和文昌鸡(P<0.01)。本研究初步表明,就新城疫和禽流感而言,不同鸡种以及性别之间的免疫性能存在明显差异。