目的 研究芍药内酯苷抗抑郁作用及其与TSPO(translocator protein 18 ku)的关系.方法 根据糖水偏好实验结果随机将小鼠分为8组:空白组、模型组(慢性不可预知应激抑郁模型)、氟西汀组,芍药内酯苷组低、中、高剂量(3.5、7.0、14.0 mg·kg-1)组、TSPO拮抗剂(PK11195)组和芍药内酯苷高剂量+PK11195组,连续给药35 d后,通过行为学实验来评估芍药内酯苷在慢性不可预知应激模型中的作用;Western blot法和ELISA法检测小鼠脑中TSPO表达量和孕酮及四氢孕酮含量,进一步明确药物作用与TSPO的关系.结果 行为学测试中,模型组与空白组均有显著性差异,说明造模成功,阳性药和芍药内酯苷不同剂量组可以逆转模型组的作用,PK11195可逆转芍药内酯苷高剂量组的作用;Western blot法和ELISA法结果显示,模型组TSPO和孕酮及四氢孕酮含量明显降低,阳性药和芍药内酯苷不同剂量组可以逆转模型组的作用,PK11195可逆转芍药内酯苷高剂量组的作用.结论 芍药内酯苷在小鼠慢性不可预知应激模型上有显著抗抑郁及抗焦虑作用,TSPO对芍药内酯苷的抗抑郁作用有一定的介导作用,这为今后进一步研究芍药内酯苷的抗抑郁作用及机制提供实验依据.

目的:观察天王补心丸的抗抑郁作用及其对雄性大鼠性功能的影响,旨在发掘天王补心丸的作用特点.方法:通过小鼠悬尾、强迫游泳和自发活动实验验证其抗抑郁药效与5-HT1A受体的关系;通过大鼠交配行为和非接触阴茎勃起实验验证药物对雄性大鼠性功能的影响,同时加入5-HT1A受体阻断剂WAY100635探讨其可能作用机制;ELISA法检测大鼠血清雄激素水平.结果:与空白组比较,天王补心丸低、中、高剂量组,氟西汀组,天王补心丸+WAY100635组小鼠悬尾不动时间显著降低(P＜0.05,P＜0.01),天王补心丸中、高剂量组,氟西汀组及天王补心丸+WAY100635组小鼠强迫游泳不动时间显著降低(P＜0.05),说明天王补心丸有显著的抗抑郁作用,且与5-HT1A受体无关.在大鼠性功能实验中,与空白组比较,氟西汀组雄性大鼠爬垮潜伏期、插入潜伏期和射精间歇时间显著延长(P＜0.01),爬垮次数、插入次数和射精次数显著减少(P＜0.01),阴茎勃起次数也显著减少(P＜0.01),而天王补心丸无此作用,合用WAY100635后氟西汀的性功能障碍作用消失;同时,所有药物对大鼠雄激素均无显著性影响.结论:天王补心丸的抗抑郁作用可能与5-HT1A受体无关,且对雄性大鼠性功能没有影响,优于氟西汀.

目的:研究天王补心丸对慢性应激抑郁模型小鼠行为学、下丘脑-垂体-肾上腺轴(HPA轴),海马糖原合酶激酶3β(GSK3β)磷酸化及脑源性神经营养因子(BDNF)表达的影响,初步探讨其抗抑郁作用机制.方法:将60只雄性ICR小鼠按照糖水偏嗜度随机分为正常组、慢性应激模型组、盐酸氟西汀组(10 mg·kg-1)以及天王补心丸高、中、低剂量组(3.6,1.8,0.9 g·kg-1).采用慢性不可预知应激实验(CUS)建立抑郁症小鼠模型,各给药组灌胃给药28 d后通过糖水偏好实验、开场行为实验、新奇抑制摄食实验检测其行为学变化.之后分别取小鼠血、肾上腺及海马组织,采用酶联免疫吸附测定(ELISA)法检测小鼠血清中促肾上腺皮质激素(ACTH)和皮质酮(CORT)含量,蛋白免疫印迹法(Western blot)检测海马组织中GSK3β磷酸化及BDNF表达的变化,并计算肾上腺指数.结果:与正常组比较,模型组小鼠的蔗糖水偏嗜度显著降低(P＜0.01),开场活动次数明显减少(P＜0.05),新奇抑制摄食潜伏期延长(P＜0.01),血清ACTH,CORT含量民明显升高(P＜0.05,P＜0.01),海马组织中GSK3β磷酸化及BDNF表达显著降低(P＜0.01),肾上腺指数显著升高(P＜0.01);与模型组比较,天王补心丸可以逆转抑郁症模型小鼠行为学变化(P＜0.05,P＜0.01),显著降低抑郁症模型小鼠血浆ACTH和CORT的含量(P＜0.01)和肾上腺指数(P＜0.01),同时增加小鼠海马GSK3β磷酸化及BDNF表达水平(P＜0.05,P＜0.01),其作用效果与盐酸氟西汀相当.结论:天王补心丸对慢性不可预知应激抑郁模型小鼠具有显著的抗抑郁作用,其作用机制与抑制HPA轴活性、上调海马GSK3β磷酸化及BDNF的蛋白表达有关.

This study aimed to investigate the antidepressant effects of Puyu Capsules and its potential mechanism. The antidepressant activity of Puyu Capsules was evaluated by forced swimming test(FST) and tail suspension test(TST) after subchronic administration in mice. Next, the mice were subjected to a chronic unpredictable stress(CUS) protocol for a period of 28 d to induce depressive-like behaviors. Then, a sucrose preference test, open-field test and novelty-suppressed feeding test were performed to evaluate the antidepressant effect of Puyu Capsules. After the behavioral test, the adrenal index was calculated; the levels of serum corticosterone(CORT) and adrenocorticotropic hormone(ACTH) were detected by enzyme-linked immunosorbent assay(ELISA); the levels of glucocorticoid receptor(GR), protein expression of brain-derived neurotrophic factor(BDNF), and the ratio of phosphorylated cAMP response element binding protein(CREB) to total CREB were detected by Western blot to explore the antidepressant function and mechanism of Puyu Capsules. The results suggested that Puyu Capsules had significant antidepressant effects on both the depression model and CUS model. At the same time, the drug could prevent the change of adrenal index induced by CUS and reverse the abnormal activation of CORT and ACTH in the serum of depressed mice. Finally, Puyu Capsules could also reverse the lower expression of pCREB, BDNF and GR in the hippocampus of CUS mice. In conclusion, Puyu Capsules produced significant antidepressant effects, and the mechanism was closely related to hypothalamic pituitary adrenal(HPA) axis activity, GR and CREB-BDNF pathway expression.

Objective:To observe the impacts of Jieyu capsule (Puyu capsule) on mice of drug-induced depression model and the possible mechanism of action.Methods:The mice were divided randomly into control group,imipramine group,Jieyu capsule (Puyu capsule) low-dosage group (235 mg · kg-1),Jieyu capsule(Puyu capsule) medium-dosage group (470 mg · kg-1) and Jieyu capsule(Puyu capsule) high-dosage group(940 mg · kg-1) according to body weights.A reserpine-induced depression model was produced through reserpine antagonism experiment,and the impacts of Jieyu capsule (Puyu capsule) on reserpine-induced body temperature drop,acinesia and ptosis were observed;then the times of mice twitching heads and fatality rate caused by yohimbine were observed through serotonine-induced head twitching experiment and yohimbine toxicity experiment,and also the impacts of drugs on serotonine and norepinephrine in mouse body were observed.Results:After being injected with reserpine,animals were reserpinized,i.e.,subjected to body temperature drop,acinesia and ptosis;Jieyu capsule of different dosages could antagonize its actions remarkably.In serotonine-induced head twitching experiment,it was observed that the times of head twitching of the mice taking Jieyu capsule(Puyu capsule) hadn't increased remarkably.In yohimbine toxicity experiment,it was observed that the mediumdosage and high-dosage Jieyu capsule(Puyu capsule) could both add yohimbine's toxicity.Conclusion:Jieyu capsule (Puyu capsule) has the anti-depression effect on reserpine-induced depression model mice;its mechanism of action is likely to be related to the enhancement of neurologic function of norepinephrine in the brain.

目的 从解郁合欢汤、血府逐瘀汤和天王补心丹3种复方水煎剂中初步筛选出有潜在抗抑郁效果的复方. 方法 取健康SPF级雄性KM小鼠50只,根据旷场试验结果随机分成空白组、盐酸氟西汀组、解郁合欢汤组、血府逐瘀汤组和天王补心丹组5组,每日灌胃给药1次,连续给药14d.每周进行悬尾试验、强迫游泳试验观测小鼠行为学变化;给药结束后测定小鼠自发活动;行为学试验结束后,用Western Blot法测定小鼠海马p-GSK3β的表达量. 结果 ①与空白组相比,血府逐瘀汤组和天王补心丹组在给药第1周和第2周均能显著缩短小鼠悬尾和游泳不动时间,作用与盐酸氟西汀组相当,其中天王补心丹组作用最显著;解郁合欢汤组有作用趋势,但无显著性差异.②3种复方均不影响小鼠自发活动.③3种复方均能增加小鼠海马p-GSK3β的表达量,天王补心丹组作用最显著. 结论 天王补心丹抗抑郁效果较强,其作用机制可能与增加海马p-GSK3β的表达有关.

目的 探讨解郁散乙酸乙酯和正丁醇有效部位的抗抑郁作用及其机制,为解郁散的进一步开发应用提供实验依据和理论基础. 方法 选择80只昆明种小鼠随机分为溶剂组、盐酸丙咪嗪组、正丁醇提取物高、中、低剂量组,乙酸乙酯提取物高、中、低剂量组共8组,每组各10只.采用小鼠悬尾试验、强迫游泳试验初步检测正丁醇提取物和乙酸乙酯提取物的抗抑郁效应,再通过5-HTP诱导的小鼠甩头试验和育亨宾毒性试验初步揭示其抗抑郁机制. 结果 小鼠悬尾不动时间和强迫游泳不动时间表明解郁散正丁醇低剂量组和乙酸乙酯高剂量组都有明显抗抑郁作用;5-HTP诱导小鼠甩头试验和育亨宾毒性试验结果发现两者均能增加小鼠甩头次数和增加育亨宾的致死率,其中正丁醇提取物和乙酸乙酯提取物中、高剂量组能明显增多小鼠甩头次数;同时,乙酸乙酯提取物均可以增加育亨宾的致死率,而正丁醇提取物中剂量组无此作用. 结论 解郁散乙酸乙酯提取物和正丁醇提取物都有抗抑郁作用,均增加了小鼠甩头次数和育亨宾毒性,说明其作用机制可能与增加突触间隙单胺递质有关.从量效关系来看:乙酸乙酯提取物的量效关系更好.

目的 应用石杉碱甲(HupA)对[Fe(CN)6]3-/[Fe(CN)6]4-([Fe(CN)6]3-/4-)体系的氧化还原作用建立石杉碱甲电化学检测的新方法.方法 采用循环伏安法和差示脉冲伏安法研究了石杉碱甲存在情况下[Fe(CN)6] 3-/4-体系的电化学行为,具体考察并优化了支持电解质、缓冲溶液的pH以及扫速等测定条件.结果 在pH 7.0的0.1 mol/L KCl-10 mmol/L K3Fe(CN)6-0.1 mol/L PBS体系中,HupA抑制的峰电流AIp与其浓度的对数值在1.00×10-7～ 4.76×10-5 mol/L范围内呈良好的线性关系,线性方程为AIp(μA)=14.19×lgCHupA(μmol/L)+14.16,线性回归系数r=0.9949.依据3σ/s计算本方法的检测限为8.82×10-8 mol/L.结论 该方法操作简便,灵敏度高,可用于实际样品中石杉碱甲的测定,其结果与高效液相法所得结果一致,效果令人满意.

OBJECTIVE To establish a rapid assay for detection of huperzine A based on gold immunochromatography assay. METHODS Colloidal gold marked coupled with monoclonal antibody against huperzine A was jet sprayed onto the glass fiber and stored at 4 . Huperzine A and anti-mouse goat immunoglobulins were jet-positioned onto a nitrocellulose membrane. Nitrocellulose membrane, gold combined pad, sample pad and absorbent paper were assembled and cut into detecting card, respectively. RESULTS The results showed that the sensitive of rapid for huperzine A visual detection limit was 15 μg·mL −1 and detection time within 5 min. Repeatability and specificity of strip was good. CONCLUSION It is very simple and convenient to rapidly detect huperzine A on the spot.

Bio-bar codes assay( BCA) has been widely used in the detection of proteins and nucleic acids. It has 5 ~ 6 orders of magnitude higher sensitivity than that of ELISA for protein targets. BCA has been shown to be extraordinarily sensitive for the detection of nucleic acid targets too. This article addressed the principle,development and application of BCA. Its prospect was looked ahead. It is important to explore the optimum reaction conditions,simplify the operation steps,reduce the test cost,and make it standardized and commercialized in the future.

OBJECTIVE To prepare and characterize monoclonal antibodies against huperzine A by establishing a determination method for huperzine A in Huperzia serrate. METHODS Balb/c mice were immunized by the conjugation of huperzine A and BSA successfully, and monoclonal antibodies were generated by hybridoma technique. Then hybridomas were cloned by the limiting dilution method and four hybridomas producing McAb reactive to huperzine A named 3E02, 4B01, 5D03 and 4C04 were obtained, an enzyme-linked immunosorbent assay for huperzine A was established successfully. RESULTS McAb isotype were IgG2b, IgG2b, IgG1 and IgG1. The ELISA standard curve was Y=0.373 6X−0.236, r=0.996 5, and the range of linearity was 5−1 000 ng·mL, and the limit of detection was 4.387 ng·mL. The recovery was 96.6%−103.8%, RSD was 0.68%(n=6). CONCLUSION The ELISA method provided a rapid, sensitive and accurate procedure for the determination of HupA in Huperzia serrata samples. KEY WORDS: huperzine A; monoclonal antibody; ELISA; Huperzia serrata (huperzine A) (Huperzia serrata) [1] ! "#$%&' ( )* +,(AChE)-./ 012345678 9:;<=> ?@8 AB 3 [2] C0 -. DEF G HI [3] J KL MN OIPQ RSTU VWXYZ 4[ L\]^G 4_ `abcJde fg hi jkl Nm$n N' opqr s tuvwxy I1 z]tu {"J |}~. de€$ ‚ƒ€ [4] „ de…†‡ iˆ‰ƒŠ C‹Œ deYŽ^‘g I1 \]^GJ 1 ( VOu .uG’ “”• 100243-200401 \]>99%)–3,3’,5,5’-— ˜™š ›(TMB) œ-20(žV Sigma Ÿ )–Sepharose Protein G(GEŸ )–¡ƒ†,¢£ƒ(¤¥¦§ ̈© aŸ “”•20120624)–«f¬(­® ̄‰ § ̈°± aŸ )–23 ́2–Lμ ¶/·3V ̧ f ́J 1o» Balb/c…† 1⁄4 6~81⁄23⁄4 ¿À‹ ÁO_Y`ÂÃ Ä `Âà ÅÆǔ• 2007000537785–SP2/0†ÈÉÊËÌ(¤¥¦§ ̈© aŸ )J`ÂOÍ ÎÀ‹Ï ÐÑÒÓÔÕ ÖÀ‹ ÁO_Y×ØÙÚÛÜ ÝG3 Huperzia serrata ÞßJ àáâ ãäàáå(žV Bio-Rad Ÿ )– Rio-Rad ,¢â 680(žV Bio-Rad Ÿ )–KQ-400 æçè1éê(ëÕìæçèâê aŸ )J 2 2.1 0íuîï ð. ñòóô 0íu 25 mg I õîö CG÷ø 25 mL ùú H3ûüï „Iýþ» J 2.2 ¶îï ð. ô Cé 1 50 ø 4Jñòóô 0.2 g 2% F 5 mL æç>ô 1 h Äô¤1ï 2% F 5 mL æ ç>ô 1 h Ä ÅC 2 ï H3 >ôïJ 2.3 iˆ‰ƒŠ .ü . ́ ‚ƒ€ -BSA de Balb/c ́ …† 1⁄4 5 ÎI !"% #$%'&'de€ ( de3de€ (0.5 mg·mL −1 )Š*+,-)!./0Þ1/% Š23Å 4 5 de…† 6 n de·3de€7./o0Þ1/%Š23ÅJ 6 nde 1 1⁄2 89:;Î< (100~ 200 μL· −1 ) =^ƒ<1 PJô P!Nm $>?B …†@ABCde -BSA î1 PBS @A Dde(1 ·d −1 ) 3 d ô EËÌ7 SP 2/0 ÈÉÊËÌ@AËÌFÅ G {" Hˆ‰ IJG KƒŠ LMÊËÌ N3 3E02 4B01 4B01 5D03 ! 4C04 C.ü 2J 2 3 000 r·min −1 Ä 30 min OP ¤Q RS TU =$Q VWXËÌ < ËÌ …ÄYZ Q[\]1^ 2 _`

Objective:To determine the content of Astraloside in Hedanshu Capsules by ELSD-HPLC.Methods:Use the DiamonsilTM(4.6mm×250mm,5μm)column,its mobile phase was acetonitrile-water(36:64).The flow rate was 1.0mL/min,drift tube temperature was 105℃,and gas flow rate was 2.7L/min.Result:It showed that the Astraloside had a good linearity within the range of 1.21~12.19μg(r=0.9998).The average recovery was 99.33%,and RSD=1.03%(n=9).Conclusion:This method is simple and accurate for determination of Astraloside in Hedanshu Capsules.

高校实验室是人才培养的重要基地,是实验教学和科学研究的重要场所。随着时代的进步和科学技术的发展,社会对人才培养提出了更高要求。药学院主要培养从事各类药物开发、生产、质量分析、合理用药及药品销售等实用型技术人才,专业能力要求较高,学生不但要学好理论知识,掌握实验技能,还要具备创新、综合

Objective To investigate the optimal extraction for compound Hedanshu capsules. Methods Orthogonal test was designed with the yields of extracts,tanshinone ⅡA,astragaloside IV as index.Results The optimal condition for extracting Radix Astragali group was 8 folds amount of water,3 times,1.5 hours each time and the optimal condition for extracting Radix Salviae Miltiorrhizae group was 6 folds amount of 60% alcohol,2 times,2.0 hours and 1.5 hours each time.Conclusion The optimized extractive technology is reproduciable and efficient with high yields.

Objective To optimize the separation and purification procedure of total flavonoids from Bauhinia championii Benth by AB-8 macroporus resin.Methods The yield of total flavonoids was used as a maker.The static and dynamic adsorption/desorption methods and orthogonal design L9(34)were used to evaluate factors such as concentration of alcohol,ratio of alcohol to raw material and ratio of resin to raw material.The content of total flavonoids was determined by UV-Vis spectrophotometer.Results The optimum conditions were as following: ratio of resin to raw material was 4,50% of alcohol was used for eluent,ratio of alcohol to raw material was 16.Conclusion This procedure is reasonable,which could be used to separate and purify the total flavonoids from Bauhinia championii Benth.

和丹舒胶囊由地龙、丹参、黄芪、牛膝等6味中药组成,具有益气利水、活血化瘀的功效,临床上用于治疗良性前列腺增生、前列腺炎等疾病[1].丹参虽为方中臣药,但用药量大,研究表明丹参中丹酚酸B是活性最强的水溶性成分,为主要质量指标.

<正>养血安神糖浆是由首乌藤、墨旱莲、熟地黄等组成,具有滋阴清热的功效,常用于治疗阴虚火旺的心悸、失眠、头晕、心烦等,收载于《中华人民共和国卫生部部颁标准》。由于原标准未对其建立质

<正>诺氟沙星是一种新型的氟喳诺酮类抗生素,具有强烈的抑菌作用,已广泛应用于临床。测定诺氟沙星在食品中残留的方法大多采用高效液相色

Based On current situation of experimental teaching and managementin our college,this paper raised some concrete approaches of imlxoving experimental teaching levels to strengthen lab construction and experimental teaching quality

Objective: To study the absorption kinetics of marine in rats′ stomachs and intestines. Methods: The drug concentration by in situ perfusion in rats were determined by HPLC. Results: The absorption percentages of marine in stomach, duodenum, jejunum, ileum and colon were (31.02±10.08)%,(21.13±2.71)%,(28.22±6.09)%,(35.66±9.01)% and(33.12±13.22)%, respectively. Conclusion: Marine is well absorbed in stomach and small intestine. The absorption characters of marine would be considered in the preparation research.