Luteolin, known for its anti-inflammatory and antioxidant properties, has garnered attention for its potential anticancer effects. Research has shown that luteolin can modulate the proliferation, migration, invasion, drug resistance, and apoptosis of digestive tract cancer cells by targeting specific pathways. This review summarizes the current understanding of luteolin’s impact on five types of digestive tract malignancies both in vitro and in vivo, elucidates its molecular mechanisms in regulating these cancers, and highlights the existing limitations and gaps in research. This analysis aims to inform the safety assessment, enhance the bioavailability, and guide the formulation development and clinical utilization of luteolin in the context of digestive tract malignancies.

PURPOSE:This study aims to comprehensively compare and analyze the burden of gastric cancer and attributable risk factors in China and Group of Twenty (G20) countries from 1990 to 2021, based on the latest the Global Burden of Disease (GBD) 2021 study. It also predicts the trends in gastric cancer incidence, mortality, and disability-adjusted life years (DALYs) in China and G20 countries over the next 19 years. METHODS:This observational longitudinal study utilizes data from the GBD 2021 study, employing indicators that include incidence, mortality, DALYs, age-standardized rates, and attributable risk factors to assess gastric cancer trends. The joinpoint regression model was used to calculate the annual average percentage change to determine long-term trends of significant changes in gastric cancer occurrence in China and G20 countries. The autoregressive integrated moving average model was employed to predict the burden trends of gastric cancer in China and G20 countries from 2021 to 2040. RESULTS:In 2021, the age-standardized incidence rate (ASIR), age-standardized mortality rate (ASMR), and age-standardized DALYs rate (ASDR) (95% uncertainty interval) for gastric cancer in China were 29.053 (22.423-36.2), 21.509 (16.663-26.611), and 501.26 (387.291-627.976) respectively, indicating a decrease compared to 1990. The ASIR, ASMR, and ASDR for G20 countries in 1990 and 2021 were substantially lower than those of China during the same periods. Joinpoint regression analysis demonstrated a significant overall decline in the APC of gastric cancer in China and G20 countries from 1990 to 2021, although a short-term upward trend was observed in China from 1998 to 2004. Predictions indicate a downward trend in ASIR, ASMR, and ASDR for both China and G20 countries over the next 19 years. However, in terms of risk factors, the proportion of DALYs due to smoking and high sodium diets in China ranked first among G20 countries in 2021. CONCLUSION:Due to the implementation of preventive strategies, advancements in healthcare, and improved economic conditions, the incidence, mortality, and DALYs of gastric cancer in China have decreased. However, there remains a certain gap compared to G20 countries at the same time. In the future, China should develop more detailed prevention and control strategies targeting risk factors, tailored to men, women, and different age groups.

PURPOSE:Using the Global Burden of Disease 2021 data, this study reports the global, regional, and national disease burden of liver cancer due to hepatitis B (LCDHB) from 1990 to 2021, stratified by age, sex, and sociodemographic index (SDI), and projects future burden to 2051. METHODS:We examined incidence, deaths, and disability-adjusted life years (DALYs) of LCDHB. Age-standardized incidence rates (ASIR), age-standardized death rates (ASDR), and age-standardized DALYs rates were analyzed (1990-2021). Spearman correlation assessed age-standardized rates-SDI relationships. The Bayesian age-period-cohort (BAPC) model projected the burden to 2051. RESULTS:Compared to 1990, the number of LCDHB incidences, deaths, and DALYs increased by 46.9, 41.2, and 33.9% in 2021; however, from 1990 to 2021, the ASIR, ASDR, and age-standardized DALYs rate all exhibited a declining trend. In 2021, the highest ASIR occurred in East Asia, High-income Asia Pacific, and Western sub-Saharan Africa. At the national and regional levels, Mongolia, the Republic of Paraguay, and the Commonwealth of the Bahamas showed peak ASIR. ASIR was higher in males and increased with age, peaking at 85-89 age group for both males and females in 2021. A reverse U-shaped correlation existed between age-standardized DALYs and SDI during 1990-2021. BAPC projections indicate declining global ASIR, ASDR, and age-standardized DALYs rates (2021-2051). CONCLUSION:Despite rising incidence, deaths, and DALYs, LCDHB treatment challenges persist, especially for males and elderly populations. Our findings on epidemiological trends and demographic variations provide crucial insights for policymakers addressing this global health burden.

Circadian rhythms are intrinsic oscillatory mechanisms in organisms exposed to day-night cycles. Recent studies highlight their complex role in tumor development. Using the Web of Science Core Collection (WOSCC), we conducted a bibliometric analysis of literature on circadian rhythm and clock genes in oncology with CiteSpace, VOSviewer, and Bibliometrix. The results reveal a general upward trend in the volume of publications over the past years. The United States leads in output and impact, with the University of California System being the most prolific institution. Levi Francis is the most prolific author. Journal analysis has identified Cancer Research as the most cited journal and Clinical Epigenetics as the most prolific. Through keyword co-occurrence and clustering analyses, we find that current research has focused primarily on anti-cancer or oncogenic effects of clock genes. Future research hotspots may be therapeutic approaches targeting the clock genes, especially focus on epigenetic modifications of circadian genes. Dual-map overlay analyses of journals revealed a shifting research trend from Molecular Biology and Genetics toward Medicine, Medical Clinical and Immunology Overall, this study provides a current analysis of the relationship between clock genes disturbances and cancer development, summarizing recent advancements and outlining future research directions.

Purpose Gastric cancer (GC) is among the malignant cancers with the highest incidence and mortality worldwide. As GC is not very sensitive to current chemotherapy drugs, there is an urgent need to develop new effective drugs. Raddeanin A (RA) is extracted from the traditional Chinese medicine Anemone raddeana Regel, which has an anti-cancer effect. The purpose of this study was to explore the effects of RA on GC in vitro and in vivo. Methods We explored the targets of RA in GC through network pharmacology. MTT assay, flow cytometry, Western blotting, and other methods were used to detect the effects of RA on the proliferation, apoptosis, and autophagy of GC cells. After preconditioning with hydroxychloroquine (HCQ) and rapamycin, we observed the effects of RA-induced autophagy on apoptosis. We further verified the antitumor effect and safety of RA in vivo. Using SNU-1 xenograft tumor model in nude mice, tumor volume was observed and liver toxicity was observed by immunohistochemistry. Results Many cancer-related signaling pathways were visualized using Cytoscape software. Among them, the MAPK signaling pathway was one of the highest-ranked pathways. The MTT assay results suggested that RA could inhibit the proliferation of HGC-27 and SNU-1 cells effectively. Flow cytometry and Western blotting confirmed that RA could significantly induce apoptosis of HGC-27 and SNU-1 cells. Electron microscopy and Western blotting demonstrated that RA could induce autophagy of HGC-27 and SNU-1 cells. Further experiments suggested that HCQ, an autophagy inhibitor, could enhance the capacity of RA to induce apoptosis. In animal studies, we found that intraperitoneal injection of RA could effectively and safely inhibit gastric tumors. Conclusions RA significantly inhibited the proliferation and induced autophagy and apoptosis of GC cells. In combination with HCQ, RA-induced apoptosis increased in vitro. The combined application of RA and autophagy inhibitors may serve as an added approach to the treatment of GC, but the underlying mechanism needs further exploration. In vivo, it was observed that RA has a good antitumor effect without increasing liver toxicity.

Emerging evidence has demonstrated marked geographical and demographic variations in prostate cancer (PC) incidence and mortality rates. As PC predominantly affects the elderly, in-depth analysis of disease trends in this vulnerable population is imperative. This study leveraged data from the Global Burden of Disease Study (GBD) 2021 to systematically evaluate temporal trends in the incidence, mortality, and Disability-Adjusted Life Years (DALYs) of PC in elderly aged 70 and above. The analyses were stratified by age group, geographic region, and Socio-demographic Index (SDI) quintiles. Furthermore, this study employed a comprehensive analytical approach, including estimated annual percentage change (EAPC), decomposition analysis, and predictive modeling (Nordpred method). From 1990 to 2021, the burden of PC in elderly exhibited a substantial rise, with incident cases, mortality cases, and DALYs all demonstrating significant increases. In 2021, the global age-standardized rates for incidence (ASIR), mortality (ASMR), and DALYs (ASDALYR) were 348.8 (95

Purpose:Solanine is the main component of the plant Solanum, which has been shown to provide growth-limiting activities in a variety of human cancers. However, little is known about its function in gastric cancer (GC).Methods:We investigated the effect of solanine on GC in vivo and in vitro. The inhibition rate of solanine on the tumor was observed by constructing a subcutaneous tumor in nude mice. Morphological changes were analyzed with H&E staining. The expression of ATF4 was detected by IF analysis. MTT assays, EdU staining, and colony formation assays were used to detect the inhibition rate of solanine on GC cells. Matrigel transwells were used to detect the invasion of GC cells. Cell migration was measured using the wound healing assay. The flow cytometric analysis was used to monitor changes in the cell cycle and cell apoptosis. Western blotting was used to detect major proteins in cells and tumors.Results:Solanine suppressed gastric tumorigenesis. Solanine also inhibited the proliferation, invasion and mitigation of GC cells, and induced cell cycle arrest and apoptosis in vitro. Moreover, the growth-limiting activities of solanine in gastric cancer were related to the suppression of the AAMDC/MYC/ATF4/Sesn2 pathway-mediated autophagy. Overexpression of AAMDC reversed the inhibitory effect of solanine on autophagy and gastric cancer.Conclusion:In summary, our findings indicate that solanine confers growth-limiting activities by deactivating the AAMDC-regulated autophagy in gastric cancer.

目的:探讨应用五禽戏之鹿戏改善前列腺癌根治术后尿失禁患者的效果.方法:选择 2021年08月至2022年08月江苏省中医院泌尿外科收治的60例前列腺癌根治术后尿失禁患者作为研究对象,按随机数字表法将患者分为观察组与对照组各30例,对照组应用盆底肌锻炼,观察组在盆底肌锻炼的基础上进行五禽戏之鹿戏功法锻炼,干预8周后记录患者每天漏尿次数以及国际尿失禁咨询委员会问卷量表(ICI-Q-SF)评分.结果:两组患者治疗后漏尿次数、ICI-Q-SF评分较治疗前有明显改善,且观察组的改善程度优于对照组,均有统计学差异(P＜0.05).结论:在常规盆底肌锻炼的基础上联合五禽戏之鹿戏功法锻炼可以减轻前列腺癌根治术后尿失禁症状,从而改善患者生活质量.

Non-small-cell lung cancer (NSCLC) is the most common cancer in the world. Previous studies have shown that Raddeanin A (RA) exhibited distinct antitumor properties in gastric and colon cancer. This study aimed to investigate the pharmacological actions and intrinsic mechanisms of RA in NSCLC. Through the application of network pharmacology, the potential targets of RA for NSCLC therapy such as SRC, MAPK1, and STAT3 were excavated. Enrichment analyses showed that these targets were concerned with the regulation of cell death, regulation of MAPK cascade, Ras signaling pathway, and PI3K/AKT signaling pathway. Meanwhile, 13 targets of RA were identified as autophagy-related genes. Our experiment data showed that RA effectively inhibited proliferation and induced apoptosis in lung cancer cells A549. We also found that RA could induce autophagy simultaneously. Furthermore, the autophagy induced by RA had a synergistic effect with apoptosis and contributed to cell death. Additionally, RA could downregulate the activity of the PI3K/AKT/mTOR pathway. Generally, our results indicated the antitumor effect and underlying mechanisms of RA on apoptosis and autophagy in A549 cells, suggesting that RA could be used as an effective antineoplastic agent.

江苏省名中医王瑞平教授,继承孟河医派学术思想,吸纳现代医学研究成果,创新恶性肿瘤治疗理念,年诊逾万,屡获佳效.笔者有幸随师学习,总结其学术经验:1)扶正为根本,健脾首当先;2)解毒以顾标,祛瘀常为用;3)辨病与辨期,个体化治疗;4)理法遵经典,方药重平和;5)中西互为参,医研相促进.

Sparganii rhizoma (SL) has potential therapeutic effects on gastric cancer (GC), but its main active ingredients and possible anticancer mechanism are still unclear. In this study, we used HPLC-Q-TOF–MS/MS to comprehensively analyse the chemical components of the aqueous extract of SL. On this basis, a network pharmacology method incorporating target prediction, gene function annotation, and molecular docking was performed to analyse the identified compounds, thereby determining the main active ingredients and hub genes of SL in the treatment of GC. Finally, the mRNA and protein expression levels of the hub genes of GC patients were further analysed by the Oncomine, GEPIA, and HPA databases. A total of 41 compounds were identified from the aqueous extract of SL. Through network analysis, we identified seven main active ingredients and ten hub genes: acacetin, sanleng acid, ferulic acid, methyl 3,6-dihydroxy-2-[(2-hydroxyphenyl) ethynyl]benzoate, caffeic acid, adenine nucleoside, azelaic acid and PIK3R1, PIK3CA, SRC, MAPK1, AKT1, HSP90AA1, HRAS, STAT3, FYN, and RHOA. The results indicated that SL might play a role in GC treatment by controlling the PI3K-Akt and other signalling pathways to regulate biological processes such as proliferation, apoptosis, migration, and angiogenesis in tumour cells. In conclusion, this study used HPLC-Q-TOF–MS/MS combined with a network pharmacology approach to provide an essential reference for identifying the chemical components of SL and its mechanism of action in the treatment of GC.

舒鹏教授行医二十余年,在长期的医疗实践中对肺癌中医药治疗形成了独特的见解,积累了丰富的临床经验,认为"正气虚损"和"气机郁滞"是肺恶性肿瘤发生发展的关键病机,治疗上采用益气健脾、疏肝理气;活血消瘀、化痰软坚;清热解毒、滋阴凉血的方法,疗效显著.

目的 利用网络药理学分析方法,基于生物信息大数据分析,以研究木兰花碱抗肿瘤的潜在的分子机制.方法 采用PharmMapper服务器挖掘木兰花碱潜在的抗肿瘤靶蛋白,通过对潜在靶蛋白参与的生物过程及KEGG通路分析,了解木兰花碱抗肿瘤的可能机制,用Cytoscape软件构建木兰花碱的"化合物-靶点-通路-疾病"相互关联网络.结果 分析结果数据,预测木兰花碱可与238个人源靶蛋白通过多种方式匹配而发挥作用,其中有75个通过20条信号通路参与肿瘤的生物过程.分别是40个靶点参与肿瘤通路,23个靶点参与多聚糖肿瘤通路,15个靶点前列腺癌通路,有13个靶点参与病毒致癌机理,有12个靶点参与化学致癌机理、胰腺癌通路、转录失调的肿瘤通路,10个靶点参与结直肠癌通路、黑色素瘤通路、非小细胞肺癌通路、肾细胞癌通路,9个靶点参与膀胱癌通路、中心碳代谢通路、子宫内膜癌通路、小细胞肺癌通路、慢性髓性白血病通路,8个靶点参与胆碱代谢通路、胶质瘤通路、急性髓性白血病通路,7个靶点参与甲状腺癌通路.主要参与凋亡调控、RNA转录调控、组织代谢、信号转导调控等生物过程中,通过FoxO signaling pathway、Ras signaling pathway、Metabolic pathways、PPAR signaling pathway等信号通路参与抗肿瘤的作用.结论 通过对木兰花碱治疗肿瘤潜在靶点的挖掘,我们推测,其可用于治疗胰腺癌、前列腺癌等多种肿瘤,为后期木兰花碱进一步深入研究提供研究方向及理论支持.

[目的]探讨黄芪建中汤加减联合甲磺酸阿帕替尼对晚期胃癌患者临床疗效及血管内皮生长因子(vascu-lar endothelial growth factor,VEGF),成纤维细胞生长因子(fibroblast growth factor,FGF)及细胞凋亡抑制蛋白存活素(Survivin)表达的影响.[方法]选择符合诊断标准的晚期胃癌患者60例为研究对象,采用SPSS软件随机生成法将患者分为观察组和对照组,每组30例.对照组给予甲磺酸阿帕替尼,观察组在对照组治疗基础上加用黄芪建中汤加减治疗.比较2组患者疗效评价、KPS评分,检测治疗前后患者血清炎症因子C反应蛋白(hs-CRP)、粒细胞集落刺激因子(G-G-CSF)、肿瘤坏死因子-α(TNF-α)水平变化,检测血清肿瘤标志物癌胚抗原(CEA)、糖类抗原199(CA199)、糖类抗原72-4(CA 72-4)水平变化及外周血中VEGF,FGF和Survivin水平变化,并观察不良反应出现情况及生存质量改善率.[结果]治疗后,观察组、对照组总有效率分别为83.33％、60.00％,2组比较差异有统计学意义(x2 = 11.9730,P = 0.020).观察组、对照组治疗后营养状况与生活质量评分均较治疗前显著升高;观察组评分高于对照组,2组比较差异有统计学意义(P＜0.05).治疗后观察组及对照组患者血清炎症因子hs-CRP、G-G-CSF及TNF-α水平均显著改变,且观察组血清炎症指标低于对照组,2组比较差异有统计学意义(P＜0.05);观察组与对照组治疗后血清肿瘤标志物CEA、CA19-9、CA72-4水平均显著降低,治疗前后比较差异有统计学意义(P＜0.05);且观察组血清肿瘤标志物下降尤为明显(P＜0.05);观察组与对照组治疗后血清VEGF、Sur-vivin、FGF水平均降低,且观察组血清VEGF、Survivin、FGF水平降低更显著,2组比较差异有统计学意义(P＜0.05);观察组较对照组不良反应显著减少,生存质量显著改善(P＜0.05).[结论]黄芪建中汤加减联合甲磺酸阿帕替尼对胃癌患者具有良好的临床疗效,其可提高KPS评分,改善患者体内血清炎症因子水平及肿瘤标志物水平,并降低血清内VEGF、Survivin、FGF水平,为提高临床疗效和患者的生活质量提供可靠基础.

目的:基于网络药理学探索川楝素对胃癌MKN-28细胞增殖的影响及其分子机制.方法:通过网络药理学筛选出川楝素治疗胃癌的分子通路;MTT法观察川楝素对人胃癌MKN-28细胞增殖抑制的量效关系;Hoechst 33258染色法观察川楝素对细胞凋亡的影响;Western Blot法检测凋亡及丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路相关蛋白表达.结果:网络药理学方法提示川楝素通过MAPK信号通路作用于胃癌,川楝素能显著抑制人胃癌MKN-28细胞增殖,并能诱导人胃癌MKN-28细胞凋亡.Western Blot实验提示,随着给药剂量增加,Cleaved Caspase-3、Cleaved-PARP、p38和p-p38表达量递增.结论:川楝素能高效抑制人胃癌MKN-28细胞增殖;川楝素是通过激活MAPK通路,诱导细胞凋亡,发挥其抗肿瘤药理作用.

王瑞平教授认为恶性肿瘤形成的病因病机复杂,正气亏虚是恶性肿瘤发病的根本原因;正虚导滞气滞血瘀痰凝,进而酿生热毒,热毒是恶性肿瘤发生的必要条件.治疗上依据恶性肿瘤的中医病因病机理论,在辨证论治与辨病论治的基础之上,利用中药的功效,突出抗肿瘤的效果.王瑞平教授通过对恶性肿瘤病因病机的理解、中药性味归经及功效主治的认识,并结合现代药理学的研究,归纳总结临床用药经验,组建了配伍合理的 8 组解毒散结的对药:金荞麦与鱼腥草、黄连与连翘、蒲公英与仙鹤草、半枝莲与半边莲、垂盆草与虎杖、山慈菇与蜂房、七叶一枝花与败酱草、全蝎与蜈蚣.对控制恶性肿瘤的发生发展、预防恶性肿瘤的复发转移,取得了良好的效果.

Although intestinal microbial dysbiosis was confirmed to be associated with many chronic diseases and health status through complicated interaction with the host, the effect on gastric cancer was less studied. In this study, we sequenced the 16S rRNA and 18S rRNA genes of fecal bacteria and fungi, respectively, in 134 gastric cancer patients and 58 healthy controls matched by age and gender. Propensity score matching (PSM) was adopted for adjusting diet habits and lifestyle, and 44 patients and 44 healthy controls (matching population) were enrolled. Serum antibody to H. pylori and metabolites of the matching population were detected. The positive rates of antibody to H. pylori between the patients and the control group did not reach the statistical difference. LEfSe analysis indicated that bacteria were more stable than fungi when adjusting diet and lifestyle. Veillonella, Megasphaera, and Prevotella 7 genus and Streptococcus salivarius subsp. Salivarius, Bifidobacterium dentium, and Lactobacillus salivarius species in bacteria were related to the risk of gastric cancer and showed a good diagnostic value in distinguishing the patients from healthy controls. Streptococcus mitis showed a risk effect for gastric cancer; however, the effect turned into be protective after PSM. Serum L-alanine, L-threonine, and methionol were positively associated with Veillonella and Streptococcus and several fungi genus. Overall, our findings indicated that fecal microbiome constitution alteration may be associated with gastric cancer through influencing the amino acid metabolism.

Gastric cancer (GC) is one of the most common cancers, and the noninvasive diagnostic methods for monitoring GC are still lacking. Growing evidence shows that human microbiota has potential value for identifying digestive diseases. The present study aimed to explore the association of the tongue coating microbiota with the serum metabolic features and inflammatory cytokines in GC patients and seek a potential, noninvasive biomarker for diagnosing GC. The tongue coating microbiota was profiled by 16S rRNA and 18S rRNA genes sequencing technology in the original population with 181 GC patients and 112 healthy controls (HCs). Propensity score matching method was used to eliminate potential confounders including age, gender, and six lifestyle factors and a matching population with 66 GC patients and 66 HCs generated. Serum metabolomics profiling was performed by ultra-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) in the matching population. Random forest model was constructed for the diagnosis of GC. Linear discriminant analysis effect size (LEfSe) revealed that the differential bacterial taxa between GC patients and HCs in the matching population were similar to that in the original population, while the differential fungal taxa between GC patients and HCs dramatically changed before and after PSM. By random forest analysis, the combination of six bacterial genera (Peptostreptococcus, Peptococcus, Porphyromonas, Megamonas, Rothia, and Fusobacterium) was the optimal predictive model to distinguish GC patients from HCs effectively, with an area under the curve (AUC) value of 0.85. The model was verified with a high predictive potential (AUC = 0.76 to 0.96). In the matching population, eighteen specific HCs-enriched bacterial genera (Porphyromonas, Parvimonas, etc.) had negative correlations with lysophospholipids metabolites, and three of them had also negative correlations with serum IL-17α. The alteration of tongue coating microbiota had a possible linkage with the inflammations and metabolome, and the tongue coating bacteria could be a potential noninvasive biomarker for diagnosing GC, which might be independent of lifestyle.

Background: Cancer stem cells (CSCs) are involved in the development of cancer. This study aimed to identify hallmark genes associated with the adjustment of CSC properties. Methods: The COAD data from The Cancer Genome Atlas(TCGA) database were assessed based on the mRNA stemness index (mRNAsi) and corrected mRNAsi. Then, both of them were analyzed with differentially expressed genes (DEGs). The gene modules were pinpointed through weighted gene co-expression network analysis (WGCNA). The genes of the most interest module were functionally annotated through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The Search Tool for the Retrieval of Interacting Genes (STRING) was adopted to obtain protein-protein interaction (PPI) networks, and the hub genes were identified in the light of the Molecular Complex Detection (MCODE) and Cytoscape plugin cytoHubba. Upstream genes were analyzed via the DisNor. In addition, the associations between clinical variables and hub genes were estimated. The bioinformatic results were verified based on Gene Expression Profling Integrative Analysis (GEPIA), Oncomine and Gene Expression Omnibus (GEO). Finally, the protein expression of the hub genes was verified by western blotting. Results: Both the corrected mRNAsi and mRNAsi increased within COAD tissues relative to those within normal colon tissues, which showed a significantly decreasing trend in COAD as the clinical stage. GO indicated the biological processes, including muscle cell differentiation, multicellular organismal signaling, muscle system process and muscle contraction. KEGG revealed Tight junction, Dilated cardiomyopathy (DCM), Vascular smooth muscle contraction, and the cGMP PKG signal transduction pathway. The seven hub genes (ACTA2, CALD1, LMOD1, MYL9, MYLK, TAGLN and TPM2) were identified in the brown module. Most of them were associated with clinical stage; MYL9, TAGLN and TPM2 were associated with overall survival. TGFB1, SRF, ROCK1 and PRKCA were the upstream genes through the DisNor. In the Oncomine, GEO and GEPIA databases, the expression of seven hub genes were down-regulated. In TCGA and GEPIA databases, the tendency of the seven hub genes was decreased with the advance in stage. Conclusions: The hub genes identified in the present study meight play a vital role in the preservation of COAD stem cells.

目的 探讨胃癌患者脾胃虚弱证形成的系统生物学机制.方法 招募22例胃癌脾胃虚弱证患者和32例体检者作为时照,基于16S rDNA和18S rDNA基因高通量测序分析舌苔细菌和真菌,电化学发光技术检测血清20种炎症因子,超高效液相色谱质谱联用技术(UPLC-MS)分析血清代谢组学.结果 与对照组相比,脾胃虚弱证患者舌苔厚壁菌门相对丰度显著升高,而拟杆菌门、变形菌门、梭杆菌门相对丰度降低(P＜0.05).线性判别分析(LDA)发现,芽孢杆菌属、肠球菌属、乳球菌属、沙雷氏菌属是胃癌脾胃虚弱证患者舌苔标志菌属.与对照组相比,胃癌脾胃虚弱证患者血清GM-CSF、IL-17α、IL-12/IL23P40和IL-6水平显著升高(P＜0.05),而IL-5、TNF-β、IL-4水平显著降低(P＜0.05),9种血清代谢分子(L-赖氨酸、二十碳五烯酸、L-天冬酰胺、溶血磷脂酰乙醇胺、磷脂酸、亚油酸、9-十六碳烯酸、α-亚麻酸和L-苯丙氨酸)显著上升(P＜0.05).Spearman相关性分析,IL-6、TNF-β、IL-5与卟啉单胞菌属、奈瑟菌属均显著相关(P＜0.05),奈瑟菌属、梭杆菌属与9种血清代谢分子均显著负相关(P＜0.05).结论 胃癌脾胃虚弱证患者舌苔菌群结构差异与血清炎症因子和代谢组学相关,为阐明脾胃虚弱证的形成机制提供新的思路与方法.