Immune checkpoint inhibitors (ICIs) have brought revolutionary therapeutic opportunities to advanced cancers. However, the insufficient accuracy of ICIs necessitates urgent advancements in precise targeting. Herein, leveraging the high compatibility of chiral configurations with chiral biomolecules, we constructed a pair of metal-centered chiral iridium(III) nanoparticles Δ-IrBMS8-NPs and Λ-IrBMS8-NPs featuring the BMS-8 moiety to enhance the chiral adaptability with immune checkpoint biomacromolecules while avoiding the use of nanocarriers. The resulting chiral nanoparticles demonstrate markedly distinct chirality-dependent biological activities. Specifically, Δ-IrBMS8-NPs achieve a 129-fold enhancement in tumor targeting, driven by the stereoselective recognition and binding of Δ-IrBMS8-NPs to tumor-specific PD-L1. This elevated targeting accuracy of Δ-IrBMS8-NPs with PD-L1 subsequently results in significantly enhanced anticancer immune responses in vivo, especially in activating the response to cytokine pathways in dendritic cells (DCs) and the recruitment of effector CD8+ T cells to remodel the tumor microenvironment. This work pioneers the discovery of nanoscale metal-centered chirality in precisely targeting immune checkpoints and highlights the pivotal role of delta configurations in enhancing anticancer immune responses, paving the way for the future rational design of effective metal-based immunotherapies.

This study employed scanning electron microscopy-energy dispersive spectrometer(SEM-EDS) and X-ray diffraction(XRD) to analyze the microstructure, elemental composition, and phase composition of different parts of "rice" and "non-rice" in Maifanitum. SEM observations revealed that the micro-morphologies of the two parts were basically consistent, mostly presenting as layered and porous, with uneven surfaces. EDS analysis indicated that the main elemental compositions of the different parts of the Maifanitum, including oxygen(O), carbon(C), and silicon(Si), were the same, but other elements such as sodium(Na), magnesium(Mg), and aluminum(Al) showed differences. The XRD analysis results indicated that the phase compositions of different parts in Maifanitum had significant differences. The main phases in the "rice" part of Maifanitum were feldspar minerals(potassium feldspar, sodium feldspar, etc.), while the main phase in the "non-rice" part of Maifanitum was quartz. XRD average patterns containing 16 and 13 characteristic peaks for "rice" and "non-rice" parts were established, respectively. The "rice" part had significant characteristic peaks of feldspar minerals(potassium feldspar and plagioclase), and the "non-rice" part had a significant quartz characteristic peak. The "rice" part of Maifanitum was mainly composed of feldspar minerals and was rich in various beneficial elements, which indicated that Maifanitum with more "rice" content had higher quality. This study provides a reference for the interpretation of the connotation of "quality evaluation through morphological identification" of mineral Chinese medicine and further enriches the scientific connotation of the "quality evaluation through morphological identification" theory.

This study aims to investigate the polarized microscopic mineral phase characteristics,inorganic element content,and potential health risks associated with the intake of raw and calcined fossil mineral Chinese medicinal material Draconis Os.Microscopy was employed to observe the mineralogical characteristics of Draconis Os and compare the microscopic features and phase composition of raw and calcined Draconis Os under monochromatic and orthogonal polarized light.Inductively coupled plasma mass spectrometry(ICP-MS)was employed to determine the content of 30 inorganic elements.Health risk assessment was conducted by calculating the single pollution index(Pi),average daily intake of elements for adults(ADI),target hazard quotient(THQ),non-carcinogenic assessment method-hazard quotient(HQ),and the carcinogenic risk of elements(CR).The results indicated that under monochromatic polarized light,the Draconis Os powder sections exhibited light gray-brown to gray-brown irregular fragments,some with undulating textures that were slightly curved.Under crossed polarized light,they appeared dark gray,grayish-white,and yellowish-white.Clear apatite was visible in the ground sections of Draconis Os under crossed polarized light.Pi results indicated that Draconis Os samples were free from contamination and were of good quality.According to the maximum allowable limits of heavy metals stipulated in ISO Traditional Chinese Medicine:Determination of heavy metals in herbal medicines used in Traditional Chinese Medicine,ADI,THQ,HQ,and CR were taken as assessment indicators.Only the THQ value for As(arsenic)in raw Draconis Os was greater than 1,while the THQ values for other heavy metal elements in the Draconis Os samples were all less than 1.The study demonstrates that the primary mineral phase of raw and calcined Draconis Os is apatite,with some samples co-existing with calcite,which can serve as one of the means for quality control of Draconis Os.The elemental analysis results from ICP-MS provide scientific evidence for the safety assessment of Draconis Os,indicating that Draconis Os is safe in clinical application.

BACKGROUND:Maifanitum, a traditional mineral medicine first documented in the "Bencao Tujing" during China's Song Dynasty (960-1279 AD), is reputed for its multifaceted therapeutic properties, including detoxification, tissue regeneration, diuretic effects, and life-prolonging capabilities. Beyond medicinal applications, Maifanitum is also utilized for water purification and health benefits by enhancing the content of beneficial elements in water. Variations in the composition and content of Maifanitum from different origins, parts, and applications may contribute to differences in its medicinal efficacy. METHODS:This study utilized X-ray Fluorescence (XRF) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to assess the material composition and loss on ignition of Maifanitum, as well as the inorganic element content within its decoction. Based on the test results, we conducted an analysis and comparison of the material composition of Maifanitum from different origins and parts using chemometric methods such as Hierarchical Cluster Analysis (HCA), Principal Component Analysis (PCA), and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA). Additionally, we assessed the quality of the Maifanitum decoction and its health risk implications by calculating ADI, THQ, HI, and CR values. RESULTS:The primary chemical constituents of Maifanitum are SiO2, Al2O3, K2O, Na2O, MnO, among others. Analyses HCA and PCA indicate a certain correlation between the chemical composition and the origin as well as the intended use of Maifanitum, with Al2O3 and MnO being the main differential components among different parts of Maifanitum. The aqueous extract of Maifanitum is predominantly composed of elements such as Na, Si, K, and Ca. An evaluation of the beneficial and harmful elements indicates that the quality of the Maifanitum decoction is significantly influenced by factors related to its origin and the part of Maifanitum. Notably, the "non-rice" portion of Maifanitum tends to receive higher scores, suggesting that a higher proportion of the "non-rice" portion may correlate with superior quality. Furthermore, the loss on ignition (LOI) of Maifanitum, ranging from 0.30 to 3.23%, implies variability in the water content among Maifanitum from different sources. CONCLUSION:This research provides significant scientific evidence for the quality control and safety assessment of Maifanitum, and there is no evidence to support that the water decoction of Maifanitum with health risk to humans.

Yuhuang Shengji ointment (YSO) is a traditional Chinese medicine clinical prescription from Professor Wang Yehuang, a famous Chinese medicine professor in Jiangsu Province, China. It is used for the treatment of postoperative healing of anal fistula in clinic practice. It is composed of Corydalis Rhizoma, Radix Sanguisorbae Carbonisata, Natrii Sulfas, Alumen Ustum, Plant Soot and Borneolum. It has the effects of eliminating dampness, astringing sores, hemostasis, pain relief, detumescence and muscle regeneration. According to the characteristics that YSO mainly contains inorganic, organic and volatile components, the comprehensive quality control of the ointment was achieved by different analytical methods. The organic components in the ointment were qualitatively analyzed by ultra performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) to determine the main chemical components. High performance liquid chromatography (HPLC) fingerprint of the organic components of the ointment was further established. Inductively coupled plasma mass spectrometry (ICP-MS) technology is used to analyze the inorganic elements in ointment, which provides data support for the quality control of inorganic components. The flash GC e-nose is used for rapid detection of volatile substances in ointment, and the fingerprint of volatile components is established. The comprehensive use of these analytical techniques not only reflects the overall chemical characteristics of the ointment, but also helps to evaluate the consistency between different batches of products. This study successfully established a comprehensive quality analysis method, which provided a basis for the quality control of YSO. At the same time, it provides a more comprehensive quality control strategy for ointment which mainly contains organic, inorganic and volatile components.

With the continuous rise of consumers' material and physical health pursuit, it is particularly critical to evaluate the safety and effectiveness of natural healthy mineral materials such as Maifanitum. The progress of modern analytical technology enables us to deeply study the internal characteristics and mechanism of these traditional mineral materials, providing a solid scientific foundation and technical support for the application of Maifanitum. A comprehensive analysis of Maifanitum was conducted using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and inductively coupled plasma mass spectrometry (ICP-MS). The ATR-FTIR analysis established a quality control fingerprint for Maifanitum, and the similarity evaluation method was employed to assess the similarity between different samples. Orthogonal partial least squares discriminant analysis (OPLS-DA) analysis revealed distinct absorption peaks among Maifanitum samples from different production areas, enabling traceability and identification. ICP-MS technology was utilized to analyze the inorganic elements in Maifanitum, revealing numerous elements closely associated with human health. These findings indicate that the established ATR-FTIR fingerprint can be effectively used to identify and control the quality of Maifanitum. The characteristics of inorganic elements in Maifanitum can not only help to distinguish its sources, but also provide a theoretical basis for the current application of Maifanitum in health care.

Arsenic trioxide (As2O3) has achieved groundbreaking success in the treatment of acute promyelocytic leukemia (APL). However, its toxic side effects seriously limit its therapeutic application in the treatment of solid tumors. To detoxify the severe side effects of arsenic, herein we synthesized innovative 2D ultrathin As2Se3 nanosheets (As2Se3 NSs) with synergistic photothermal-triggered immunotherapy effects. As2Se3 NSs are biocompatible and biodegradable under physiological conditions and can release As(III) and Se(0). Furthermore, selenium increases the immunomodulatory efficacy of arsenic treatments, facilitating reprogramming of the tumor microenvironment by As2Se3 NSs by enhancing the infiltration of natural killer cells and effector tumor-specific CD8+ T cells. The synergistic combination of photothermal therapy and immunotherapy driven by As2Se3 NSs via a simple but effective all-in-one strategy achieved efficient anticancer effects, addressing the key limitations of As2O3 for solid tumor treatment. This work demonstrates not only the great potential of selenium for detoxifying arsenic but also the application of 2D As2Se3 nanosheets for cancer therapy.

This paper presents an optimized preparation process for external ointment using the Definitive Screening Design (DSD) method. The ointment is a Traditional Chinese Medicine (TCM) formula developed by Professor WYH, a renowned TCM practitioner in Jiangsu Province, China, known for its proven clinical efficacy. In this study, a stepwise regression model was employed to analyze the relationship between key process factors (such as mixing speed and time) and rheological parameters. Machine learning techniques, including Monte Carlo simulation, decision tree analysis, and Gaussian process, were used for parameter optimization. Through rigorous experimentation and verification, we have successfully identified the optimal preparation process for WYH ointment. The optimized parameters included drug ratio of 24.5%, mixing time of 8 min, mixing speed of 1175 rpm, petroleum dosage of 79 g, liquid paraffin dosage of 6.7 g. The final ointment formulation was prepared using method B. This research not only contributes to the optimization of the WYH ointment preparation process but also provides valuable insights and practical guidance for designing the preparation processes of other TCM ointments. This advanced DSD method enhances the screening approach for identifying the best preparation process, thereby improving the scientific rigor and quality of TCM ointment preparation processes.

Background: Epilepsy is a neurological disease, and its clinical treatment presents a significant medical challenge. Nevertheless, Chloriti Lapis (CL), a traditional Chinese Mineral Medicine, has been employed for centuries to address neurological disorders and has shown notable effectiveness. Objectives: The objectives of this study are to further explore whether CL can modulate the abundance and diversity of the small intestinal flora in rats with epilepsy induced by pentylenetetrazol (PTZ) and to investigate the interrelationship among the flora. Materials and Methods: Operational taxonomic units (OTUs), α diversity, β diversity, hierarchical clustering tree, and principal component analysis (PCA) were utilized to analyze the small intestinal flora of PTZ-kindled epileptic rats. Results: The predominant bacterial groups in the small intestine mainly belonged to the phyla Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. The administration of CL was found to effectively increase the abundance and diversity of the small intestinal flora, modify its structural composition, reduce the relative abundance of PTZ-elevated microflora, and enhance the abundance of Streptococcus, Staphylococcus, and Gemella Berger. Conclusion: CL exhibits promising potential, reliability, and unique value in managing epilepsy through the regulation of intestinal flora.

Background: Maifanitum, a mineral used in Chinese medicine, was first documented during the Song Dynasty (960-1279). Historical records suggest its multifaceted therapeutic properties, including detoxification and stasis resolution, necrosis removal and tissue regeneration, diuretic and calculi dissolution and prolonging life. The concentration of elements in Maifanitum may vary depending on its origin, different parts, which can affect its effectiveness in different fields of applications. Therefore, the analysis of elements in Maifanitum and the subsequent health risk assessment have been conducted. This provides an important basis for the quality control and application safety of Maifanitum. Method: The analytical techniques employed in this study are inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES), utilized for the quantitative assessment of 60 elements (Refer to Appendix 1) within Maifanitum samples. Based on the test results, chemometric methods are employed to evaluate the characteristics and differences in elemental concentration from different sources and locations. Additionally, a preliminary health risk assessment is conducted for Maifanitum from different origins and various parts. Results: We have established a fingerprint of the elements within Maifanitum, demonstrating a commendable level of similarity. The findings from hierarchical cluster analysis(HCA) corroborated with those from principal component analysis (PCA), collectively unveiling a systematic profile of elemental disparities between Maifanitum samples of diverse origins and applications. It also revealed that there are differences in the concentration of Al, Ga, Be, Hf, Na, Sn, Ti, Zr, Gd, Tb, Sr, Pb, Ce, Ba and other elements in different parts of Maifanitum. While Cd, As, and Cu levels in all samples were within the permissible limits as defined by the Chinese Pharmacopeia, Pb concentrations in the majority of samples were found to surpass these standards, albeit slightly in the "non-rice"fraction. The assessment of both beneficial and deleterious elements indicates that the "nonrice"fraction of Maifanitum possesses superior quality attributes. Moreover, the overall concentration of rare earth elements in Maifanitum is substantially below the established lower threshold for daily human consumption, with no immediate evidence suggesting any adverse health risks. Conclusion: This study provides a basis for the quality control and safety evaluation of Maifanitum in clinical use.

Ethnopharmacological relevanceRealgar, a traditional mineral Chinese medicine, has been used in China for more than 2000 years. It has been recorded in many ancient and modern works that it has anti-cancer and anti-tumor effects. Of course, colon cancer is also within the scope of its treatment. Realgar needs to be processed into realgar decoction pieces by water grinding before being used for medicine. To ensure the consistency of efficacy and quality of realgar decoction pieces, modern methods need to be used for further quality control.Aim of the studyThe research of traditional mineral Chinese medicine is relatively difficult, and the related research is less. The purpose of this study is to control the quality of realgar decoction pieces by modern analytical technology and analyze its components. On this basis, its anti-colon cancer activity was discussed.Materials and methodsSeveral batches of realgar decoction pieces were analyzed by XRD, and the components of realgar decoction pieces were obtained. The quality control fingerprints of realgar decoction pieces were established by processing XRD spectra and similarity evaluation. Then, the effects of realgar decoction pieces on apoptosis of CT26 and HTC-116 cells were observed in vitro by Hoechst 33258 staining, flow cytometry, measurement of mitochondrial membrane potential and Western blot; In vivo, the mouse model of tumor-in-situ transplantation of colon cancer was established, and the related indexes were observed.ResultThe explorations showed that the XRD Fourier fingerprints of realgar decoction pieces samples that had the same phase revealed 10 common peaks, respectively. The similarity evaluation of the established XRD Fourier fingerprint was greater than 0.900. We also demonstrated that realgar decoction pieces can promote apoptosis and inhibit tumor growth in colon cancer cells, its activating effect on p53 protein, and its safety when used within reasonable limits.ConclusionThe quality control of realgar decoction pieces by XRD is scientific and has the inhibitory effect on colon cancer, which has the development potential.

Background Epilepsy is a neurological disease, and its clinical treatment presents a significant medical challenge. Nevertheless, Chloriti Lapis (CL), a traditional Chinese Mineral Medicine, has been employed for centuries to address neurological disorders and has shown notable effectiveness. Objectives The objectives of this study are to further explore whether CL can modulate the abundance and diversity of the small intestinal flora in rats with epilepsy induced by pentylenetetrazol (PTZ) and to investigate the interrelationship among the flora. Materials and Methods Operational taxonomic units (OTUs), α diversity, β diversity, hierarchical clustering tree, and principal component analysis (PCA) were utilized to analyze the small intestinal flora of PTZ-kindled epileptic rats. Results The predominant bacterial groups in the small intestine mainly belonged to the phyla Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. The administration of CL was found to effectively increase the abundance and diversity of the small intestinal flora, modify its structural composition, reduce the relative abundance of PTZ-elevated microflora, and enhance the abundance of Streptococcus, Staphylococcus, and Gemella Berger. Conclusion CL exhibits promising potential, reliability, and unique value in managing epilepsy through the regulation of intestinal flora. Keywords Traditional Chinese medicine , Mineral Chinese medicine/mineral drug/mineral medicine , , , epilepsy , pentylenetetrazol , small intestine , gut microbiota , bain-gut axis , mechanism of action

Endothelial cell dysfunction caused by inflammation and even vascular leakage are important manifestations of blood stasis syndrome (BSS). Reversible regulation of vascular integrity to cure BSS has attracted considerable interest. Herein, a novel acidic polysaccharide (TPP-4) was purified and characterized from Typhae Pollen, a typical traditional Chinese medicine for treating BSS, especially for bleeding caused by blood stasis. A series of structural characterization methods, including spectroscopic methods (FT-IR and UV), chromatographic methods (HPGPC, HPAEC-PDA and GC-MS) and NMR, have been used to reveal the fine structure of TPP-4. TPP-4 was a homogeneous heteropolysaccharide comprised with RG-I backbone. TPP-4 showed fantastic activities in vascular integrity regulation both in vitro (HUVECs) and in vivo (zebrafish). Transcriptomics revealed that SOX7 and lipid metabolism were the potential targets. Lipidomics showed that TPP-4 could regulate lipid metabolism disorders caused by vascular inflammation, particularly affecting LPE levels. The above regulatory effects were furtherly demonstrated to be related with VEGFA/PI3K/mTOR signaling pathway through various molecular biological experiments.

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The interactions between human serum albumin (HSA) and the hemostatic components of the Chinese medicine Sanguisorbae Radix (SR), specifically phenolic acid compounds such as caffeic acid (CA), ferulic acid (FA) and their 1:1 mixture (1:1) were studied to investigate the molecular mechanism underlying the hemostatic effect of SR. Network pharmacology combined with the experimental and computational data revealed that HSA is one of the hemostatic targets to SR phenolic acids. SDS-PAGE and multi-spectroscopy demonstrated that the phenolic acids bind to the Sudlow site I on HSA, altering its structure and influencing its migration velocity. There is an observed synergistic effect upon the mixture of CA and FA. Quantum chemistry, molecular docking, and molecular dynamics simulations indicate that the binding of phenolic acids to HSA is stable, and variations in binding efficiency are associated with the hydrophobicity of the substituent at the C3 position of the side chain, and also, the key amino acids and functional groups for hemostasis of SR were identified, along with the active sites that contribute to the synergistic enhancement by phenolic acids.

Background: Realgar is a mineral Chinese medicine, which has been used in China for thousands of years. A well-known truth is that the quality of traditional Chinese medicines is the basis for their efficacious and reliable roles for clinical use, which is principally determined by elements including the location of production and inner inorganic elements composition. However, there are few studies on the quality control of Realgar at present. Colon cancer, a severe malignant tumor, is known to have a poor prognosis due to a high rate of metastasis. So far, the effects of Realgar against colon cancer have not yet been clarified.Methods: An appropriate X-Ray Diffraction (XRD) method was used to systematically analyze the Realgar medic-inal material samples. Fourier fingerprint combined with similarity evaluation, hierarchical cluster analysis, and principal component analysis was performed to assess and classify the samples on account of the XRD Fourier data. Cell viability, wound-healing, transwell migration, and matrigel invasion assays were performed. West-ern blotting was applied to determine the expression of the epithelial-mesenchymal transition (EMT) and MMPs relevant protein levels. Results: Our explorations showed that the XRD Fourier fingerprints of Realgar medicinal material samples that had the same phase revealed 15 and 12 common peaks, respectively. The similarity evaluation of the established XRD Fourier fingerprint was greater than 0.900, which indicated that the XRD Fourier fingerprints of Realgar medicinal material were successfully established. Our study demonstrated that Realgar inhibited the proliferation, migration, and invasion of colon cancer cells. Realgar suppressed the EMT process and decreased MMP-9 and MMP-2 levels, which could be the reason for Realgar to inactivate colon cancer cell migration and invasion.Conclusions: Our findings could be applied in the quality control of Realgar and strongly demonstrate that Realgar could be a potential anti-tumor agent for colon cancer treatment.

Arsenic(II) sulfide is a stable inorganic arsenic compound with a different valence from arsenic trioxide, and has been widely applied to treat various diseases with low toxic side effects for a long time. However, its low solubility and complicated formulations restrict its further applications in modern medical industry. Meanwhile, as the tumour with the highest incidence rate among women, the low recurrence risk of breast cancer has been confirmed to be closely related to the high infiltration of immune cells. Herein, we synthesized and filtered novel biocompatible PEGylated arsenic(II) sulfide nanocrystals AsS@PEG with a size of 93.14 ± 0.49 nm by the gel method, which displayed excellent anticancer and immune activation activity in breast cancer model. Proteomic analysis suggested that the AsS@PEG induce ferroptosis in cancer cells and further activate antitumour immune responses via B-cell lymphoma 9-like (BCL9L) protein inhibition. Furthermore, mechanism studies revealed notable glutathione peroxidase 4 (GPX4) downregulation in cancer cells, dendritic cells (DCs) maturation and subsequent effector CD8 + T-cells production induced by the AsS@PEG in the tumour microenvironment. This study highlights biocompatible arsenic(II) sulfide nanocrystals that induce ferroptotic cell death and activate antitumour immune responses, providing insights into the path towards the immunotherapy assisted chemotherapy for breast cancer.

ETHNOPHARMACOLOGICAL RELEVANCE:Spatholobus suberectus vine stem (SSVS) is the dried lianoid stem of the leguminous plant, Spatholobus suberectus Dunn, which is mainly distributed in China and some Southeast Asian countries. Due to its notable effects of promoting blood circulation and tonifying blood, regulating menstruation and relieving pain, this phytomedicine has been used in traditional Chinese medicine for hundreds of years.AIM OF THE STUDY:This review is designed to provide a comprehensive profile of SSVS concerning its botany, traditional uses, phytochemistry, quality control, pharmacology, pharmacokinetics, and toxicology and attempts to provide a scientific basis and future directions for further research and development.MATERIALS AND METHODS:Related document information was collected with the help of databases such as the Web of Science, Science Direct, PubMed, China National Knowledge Infrastructure (CNKI) and Flora of China.RESULTS:SSVS is reported to be traditionally used to treat rheumatic arthralgia, numbness and paralysis, blood deficiency, irregular menstruation and other gynecological diseases. Botanical studies have revealed that there are some confusable varieties in some specific locations with a long history. Additionally, 145 chemical constituents have been isolated and identified from SSVS, including flavonoids, organic acids, terpenoids, lignans, and phenolic glycosides. Pharmacological studies have shown that SSVS has a variety of effects, such as nervous system regulation, and antioxidative, antitumor, antiviral, antidiabetic, and anti-inflammatory effects. However, in regard to the absorption-distribution-metabolism-elimination-toxicity (ADMET) of SSVS, few studies have been carried out, and few articles have been published.CONCLUSION:With a long history of traditional uses, a variety of bioactive phytochemicals and a wide range of definite pharmacological activities, SSVS is believed to have great potential in clinical applications and further research, development and exploitation. The precise action mechanisms, rational quality control and quality markers, and explicit ADMET routes should be highlighted in the future, which might provide effective help to safely, effectively and sustainably use this herbal medicine.

OBJECTIVE To establish, analyze and evaluate the inorganic element fingerprint of mineral medicine realgar prepared by water grind processing, and to conduct in vitro anti-colon cancer activity research to explore the spectrum-effect relationship between various inorganic elements and the inhibition of colon cancer cell proliferation.METHODS Inductively coupled plasma-mass spectrometry（ICP-MS） was used to determine the contents of 30 inorganic elements in 12 different batches of realgar prepared by water grind processing to establish inorganic element fingerprints, and the similarity was evaluated. MTT method was used to determine the proliferation inhibition rate of realgar on colon cancer HCT116 cells, and partial least squares regression（PLSR） and gray relational degree（GRA） were combined to analyze the spectrum-effect relationship.RESULTS Among the 30 elements detected, the contents of As, Fe, Sb, Al and Mg were relatively high, while Na, Zn and heavy metals Cu and Cd were not detected, while Pb and Hg were partially detected. The characteristic fingerprints of 12 inorganic elements were established, and the similarity（cosine value of the included angle） was all greater than 0.900 0.The 12 batches of realgar were divided into 2 categories, and the comprehensive evaluation score of realgar from Guizhou ranked in the forefront. MTT experiments showed that realgar inhibited the proliferation of colon cancer HCT116 cells. The partial least squares regression analysis and gray correlation analysis showed that the elements with VIP ＞ 1 and correlation degree ＞ 0.7 were As, Sr, Ga, Ti, Al, Fe, V, Sb, Co, Mg and Mn.CONCLUSION There are certain differences in the types and contents of inorganic elements in different batches of realgar prepared by water grind processing. This study established an accurate and efficient method for analyzing and evaluating inorganic elements in realgar prepared by water grind processing, and the anti-colon cancer effect of realgar is the result of the synergistic action of various elements, mainly closely related to the 11 elements of As, Sr, Ga, Ti, Al, Fe, V, Sb, Co, Mg and Mn.

目的:调查分析华东地区矿物类中药(以下简称矿物药)资源种类、分布及应用现状,为我国矿物药资源发展战略及华东地区区域性矿物药资源产业化发展规划的制定、完善及生产布局提供参考.方法:通过文献检索结合实地调研方式对华东地区矿物药资源进行调查,并对调查数据进行归类和分析.结果:华东地区分布矿物药品种共156种,其中文献资料记载品种141种、现有分布品种138种,文献记载与现有分布共有品种123种.结论:基本查明了华东地区药用矿产资源概况,该区域分布药用矿产资源品种较为丰富,但临床应用品种较少,资源产业化有较大的发展空间;基本厘清了华东地区矿物药资源概况,有助于后续进一步挖掘该地区药用矿产资源的药用价值、发展区域地质经济.