<正>马来酸氯苯那敏(又称扑尔敏)是重要的抗过敏类非处方药品,临床上广泛用于治疗各种过敏性疾病,是常用的抗感冒药的组方成分。目前,高效液相色谱法(HPLC)是制剂中马来酸氯苯那敏的含量测定的主要方法,但目前HPLC定量分析马来酸氯苯那敏的方法存在着明显的问题或争论。由于在色谱

Based on the single-factor test analysis,the extraction technology parameters,including extraction temperature,extraction time and material to solvent ratio(MSR) were optimized with response surface methodology.The parameters were as follows: extraction temperature 94 ℃ extraction time 4.7 hours,ratio of material to solvent 1 ∶ 21(g/mL).Under those conditions,the predicted value of polysaccharide extraction yield from peach blossoms was 11.30 %,while the actual value is 11.08 %.

OBJECTIVE: To establish spectrophotometry and HPLC methods for the determination of ferrous succinate tablet and to compare the results of above two methods with that of cerium sulphate method.METHODS: Ferrous reacted with 1,10-phenanthroline to form colored complex and spectrophotometry was applied to measure its absorbance.The detection wavelength was set at 510 nm.HPLC method was performed on C18 column with mobile phase consisted of 0.05 mol·L-1 KH2PO4 (pH=2.5) at detection wavelength of 215 nm.The method was quantified by an external standard mode.RESULTS: The linear range of ferrous in spectrophotometry was 0.042 44～2.546 4 mg·L-1 (r=0.999 8) with an average recovery of 99.5% (RSD<1.9%).Good linearity of ferrous was obtained (r=0.999 0) by HPLC within the range of 0.25～32.2 mmol·L-1 with an average recovery of 99.8% (RSD<2.1%).The average values obtained by cerium sulphate method,spectrophotometry and HPLC method were 36.28%,36.73% and 29.13%,respectively.CONCLUSIONS: There is no significant difference in detection of total ferrous between spectrophotometry and cerium sulphate method.Both methods can be used for the quality control of ferrous succinate tablet.HPLC can be used for the content determination of ferrous succinate.

Objective To study the extraction and identification of total flavonoids from Eriocaulon buergerianum Koern and its Scavenging activity on hydroxyl free radical.Methods The total flavonoids from Eriocaulon buergerianum Koern was extracted with ethanol as the solvent by ultrasonic.The quality of extracted flavonoids was identified by chemical methods,and the content of total flavonoids was determined with spectrophotometry.In addition,the effect of extracted flavonoids on hydroxyl free radical was investigated.Results The content of total flavonoids from Eriocaulon buergerianum Koern was 5.09% and the recovery rate was 102.1%.Conclusion It is an ideal way to extract flavonoids from Eriocaulon buergerianum Koern.The extracted flavonoids show strong scavenging activity on hydroxyl free radical.The results provide a theoretical basis for further development and use of Eriocaulon buergerianum Koern.

Objective To synthesize functionalized CdS quantum dots(QDs) and to study their interaction with bovine serum albumin(BSA).Methods Three kinds of functionalized CdS QDs were successfully synthesized with thiourea(Tu), mercaptoacetic acid(TGA) and L-cysteine(L-Cys) in aqueous solution at room temperature.The size and morphology of them were determined by UV-Vis absorption spectrum and transmission electron microscopy(TEM).Fluorescence properties of the QDs and the interaction between L-Cys-CdS QDs and bovine serum albumin(BSA) were also studied by fluorescence spectroscopy.Results The method can be used to obtain quickly and easily three kinds of functionalized CdS QDs with more stable and stronger fluorescent performance.And electrostatic or coordination effect may exist between L-Cys-CdS QDs and BSA. Conclusions The functionalized CdS QDs can be used as fluorescence probe in detecting biological samples.The results will be the basis for studying the interaction between the QDs and biological macromolecules.

To study the stability of glucosamine-α-aminoacids-copper(Ⅱ) ternary complexes,the formation constant of α-aminoacids-glucosamine-copper(Ⅱ) ternary complexes were determined at 298 K,I=0.1 mol/L KNO_3 by pH method.It was found that the two biologically active ligand,α-aminoacid and glucosamine accommodate each other when coordinated to copper(Ⅱ) ion.The stability of the ternary complexes is higher than that of the corresponding binary complex.The formation constant of the ternary complexes linearly increases with increase of the protonation constant of aminoacid and the stability of its binary complexes.Linear free energy relationship(LFER) does exist in the ternary system.

Objective To explore an optimum extraction technology of essential oil from peach blossom.Methods Orthogonal design L9(34) was carried out to find the optimum extraction technology by detecting the extracting ratio,among the soaking time(A),extracting time(B),adding water(C) and breaking-up degree of the blossoms(D).Results In all designs,the extracting ratio was the highest when the soaking time was 3h(A2),extracting time was 3 h(B2),adding water quality was 6 times(C3),the peach blossom was broken.Conclusion The optimum extraction technology is A2B2C3D3.and it is stable and feasible.

Objective To study chemical constituents of the roots in the Aster ageratoides var.oophyllus.Methods The extracted compounds from the plants were separated and purified by means of silica gel column chromatography(CC) and elucidated by spectroscopic experiments(~1H-NMR,~(13)C NMR,HMBC,MS,UV,IR) to confirm the structures in the compounds.Results Four compounds were obtained and established as 2β,3β,16α-trihydroxy-23α-al-12-en-noroleane(I),(24R)-5α-stigmast-7,22(E)-dien-3α-ol(II),α-sitosterol(III),glycerin 1,3-dilinoleate(IV).Conclusion Compound I was new and II-IV were all isolated from this plant for the first time.

本文建立了在等度洗脱下高效液相色谱法(HPLC)测定血清中同型半胱氨酸含量的方法.血浆同型半胱氨酸经巯基乙醇还原后用H2O2氧化巯基,与邻苯二甲醛(OPA)衍生,经C18 色谱柱在45℃分离,荧光检测.方法简便、快速,线性范围为0.685～148.0 μmol*L-1,r=0.9998;方法回收率为96.4%～100.1%.日内相对标准偏差小于5%,日间相对标准偏差小于6%.

用原子吸收分光光度法对乌梅叶和果进行8种微量元素的测定.结果表明:乌梅叶和果部分均含有丰富的人体必需的微量元素,具有较高的药用价值.

目的对卵叶三脉紫菀中三萜及甾体成分进行系列研究.方法利用柱层析进行化合物分离,用波谱法鉴定其结构.结果从此植物中分离到4个化合物,经结构鉴定分别为波甾醇、表木栓醇、β-谷甾醇、豆甾醇-β-D-葡萄糖甙.结论 4个化合物均为首次从此植物分离而得.

Objective To determine the content of total flavone in scutellaria barbata grown in Huixian.Methods The total flavone was extracted from scutellaria barbata using 70% C 2H 5OH.The content of total flavone was determined by ultraviolet spectrophotometry,and compared with rutin complex with Al 3+ at 271nm.Results The average content of total flavone was 4.11%.The results of recovery show that the method of this article was convenient, practicable and the results was reliable.Conclusion The content of total flavone of Huixian is in accord with the products in other growing places.

The content of mineral elements and amino acids in Aster tataricus was determined. The results showed that the contents of Cu?Zn?Fe?Mg?Ca?Mo?Mn and Se were 42.89?58.14?920.93?2548.25?3925.43?0.65?52.18 and 0.0005mg·Kg -1 respectively. The contents of amino acids of ASP?Glu?Gly?Thr?His+Arg?Ala?Met?Val?Phe?Ile?Leu and Lys were 26?17?40?36?190?36?37?80?8.7?4.5?13 and 41 mg·Kg -1 respectively.

用原子吸收分光光度法测定高莴苣和多裂山莴苣的8种微量元素,结果表明高莴苣和多裂山莴苣均含有丰富的人体必需的微量元素,为进一步研究和综合利用提供了新的科学依据.

Objective To determine the contents of free amino acids in fresh Ficus carica fruit.Methods Concentrations of 15 kinds of amino acids in Ficus carica fruit were determined by reversed-phase high performance liquid chromatography(RP-HPLC),with OPA and 2-mercapyo ethanol as precolumn derivative reagents.Gradient elution and fluorescence detector was used(λex=335nm,λem=430nm).Results Fifteen kinds of amino acids had been found, and there were many kinds of essential amino acids needed by human body in Ficus carica fruit.The contents of essential amino acids in the total free amino acids were 45.06% and concentrations of Ala, Ser, Lys and Asp were slightly higher.Conclusion Amino acids are rich in Ficus carica fruit which is valuable as special diet.

用原子吸收分光光度法测定多裂山莴苣中的Fe、Cu、Mn、Mg、Se、Ge、Mo等8种微量元素，结果表明，多裂山莴苣含有丰富的人体必需的微量元素，为多裂山莴苣的进一步研究和综合利用提供了新的科学依据。

用原子吸收分光光度法测定了紫菀和山紫菀中8种微量元素的含量。结果表明：紫菀和山紫菀含有丰富的人体必需的微量元素，具有较高的药用价值。

用原子吸收分光光度法测定半枝莲中的Fe、Cu、Zn、Mn、Mg、Se、Ge、Mo等8种微量元素。结果表明：半枝莲含有丰富的人体必需的微量元素，为半枝莲的进一步研究和综合利用提供了新的科学依据。

合成了富勒烯硅油,并首先将其用做固相微萃取(SPME)涂层,利用顶空固相微萃取与气相色谱联用技术(HS-SPME-GC)分析了水相中5种邻苯二甲酸酯,对实验条件诸如离子强度、吸附时间和热解吸时间进行了研究;与商用PDMS萃取头比较,结果显示该萃取头对高沸点半挥发性的邻苯二甲酸酯的萃取选择性优于PDMS.该方法的线性范围在0.001～1000μg/L,检出限在8.4×10-4～23.8μg/L,相对标准偏差均在15%以下.

用原子吸收分光光度法测定柳絮中的Fe、Mn、Cd、Zn、Cu、Ca、Ni、Pb等8种微量元素,结果表明:柳絮中含有丰富的微量元素,可作为食物对人体补充微量元素,也为柳絮药效的进一步研究和综合开发利用提供了新的科学依据.