alpha-Synuclein (alpha-syn) aggregation is a hallmark of Parkinson's disease (PD) pathology. In blood, alpha-syn aggregates (alpha-syn Agg) exist either freely (free-alpha-syn Agg) or bound to neuron-derived extracellular vesicles via L1CAM (L1EV-alpha-syn Agg). However, the lack of sensitive tools to distinguish these two forms hampers PD diagnostics. Here, a dual-channel biosensor that simultaneously detects free-alpha-syn and L1EV-alpha-syn Agg in serum using ultra-bright optical signals is introduced. The biosensor employs specific antibody pairs on magnetic beads and aggregation-induced emission (AIE) fluorophores to generate distinct orange-red and yellow-green fluorescence for each alpha-syn Agg type, forming stable immunosandwich complexes. It performs robustly in both liquid-phase and solid-phase assays. With AI-assisted image recognition, it enables rapid and accurate detection of alpha-syn Agg based on dual-color signatures. This strategy allows for effective differentiation of PD from healthy controls in clinical samples, achieving an AUC of 0.98 (specificity = 0.96, sensitivity = 0.90). By integrating dual dimensions of alpha-syn pathology, this method offers a promising tool for precise, non-invasive PD diagnosis, and sets the foundation for future clinical translation in neurodegenerative biomarker detection.

Objective(s): Dexmedetomidine (Dex) is a potent alpha 2-adrenergic receptor(alpha 2-AR) agonist that has been shown to protect against sepsis-induced lung injury, however, the underlying mechanisms of this protection are not fully understood. Autophagy and the Smad2/3 signaling pathway play important roles in sepsis-induced lung injury, but the relationship between Dex and Smad2/3 is not clear. This study aimed to investigate the role of autophagy and the Smad2/3 signaling pathway in Dex-mediated treatment of sepsis-induced lung injury. Sepsis was performed using cecal ligation and puncture (CLP) in C57BL/6J mice. Materials and Methods: Mice were randomly assigned to four groups (n=6 per group): sham, CLP, CLP-Dex, and CLP-Dex-YOH, Yohimbine hydrochloride (YOH) is an alpha 2-AR blocker. The cecum was carefully separated to avoid blood vessel damage and was identified and punctured twice with an 18-gauge needle. The pathological changes, inflammatory factor levels, oxidative stress, autophagy, Smad2/3 signaling pathway-related protein levels in lung tissues, and the activity of superoxide dismutase (SOD) and malonaldehyde (MDA) in the serum were measured. Results: CLP-induced lung injury was reflected by increased levels of inflammatory cytokines, apoptosis, and oxidative stress, along with an increase in the expression of autophagy and Smad2/3 signaling pathway-related proteins. Dex could reverse these changes and confer a protective effect on the lung during sepsis. However, the administration of YOH significantly reduced the positive effects of Dex in mice with sepsis. Conclusion: Dex exerts its beneficial effects against sepsis-induced lung injury through the regulation of autophagy and the Smad2/3 signaling pathway.

Abstract Objective P-type atypical lymphocytes may play important roles in the aetiology and therapy of schizophrenia. However, there is merely a direct immunological characterisation of it. The aim of this study is to explore the surface antigens of these cells and their comparative ultrastructure in schizophrenia. Methods We recruited 25 age-and gender-matched patients with unmedicated schizophrenia, other mental diseases and healthy individuals. Peripheral venous blood was smeared and stained. CD4+, CD8+ and CD19+ cell surface antigen- positive lymphocytes were purified using magnetic beads and prepared for light microscopy and electron microscopy. Results The percentages of P-type atypical lymphocytes (34.53% ± 9.92%) were significantly higher (p < 0.0001) in schizophrenia than that of other mental diseases (9.79% ± 3.45%). These cells could present CD4+, CD8+ and CD19+ surface antigens. Their relative ultrastructure differed from that of normal lymphocytes, especially in mitochondria, which showed abundant, aggregated and quite irregular mitochondria; for example, slight dilation of the foci, swelling, degeneration, and even cavity. Conclusions P-type atypical lymphocytes could be found among CD4+, CD8+, and CD19 + lymphocytes with schizophrenia. Their abnormal ultrastructure of mitochondria implied that energy metabolism might play an important role in the aetiology of schizophrenia.

Background:The effects of realgar against non-small cell lung cancer (NSCLC) have been massively studied, but the direct therapeutic targets of realgar remain unclear. This study aimed to identify the molecular targets of realgar against NSCLC and explore their therapeutic mechanisms based on a network pharmacology approach and experimental validations.Methods:The BATMAN-TCM and Digsee databases were used to predict realgar targets and NSCLC-related genes, respectively. A protein-protein interaction network was constructed for each gene set, and the overlapping genes were identified as potential targets of realgar against NSCLC. The correlation between potential targets and NSCLC was analyzed using The Cancer Genome Atlas and International Cancer Genome Consortium databases, and the key target was validated by in-silico and in-vitro experiments.Results:Twenty-three overlapping genes, including xanthine oxidase (XO), were identified as potential targets of realgar against NSCLC. XO was selected as the key target for validation, as it was found to be upregulated in NSCLC tumor tissue, which correlated with poor overall survival. A possible interaction between realgar and XO was revealed by molecular docking which was further validated experimentally. Realgar treatment suppressed the activity of XO in NSCLC cells, as demonstrated by the unchanged XO protein levels. Finally, the mechanism of action of XO as a target against NSCLC through the cell-cell junction organization pathway was investigated.Conclusions:Overall, this study proposes a potential molecular mechanism illustrating that XO is a target of realgar against NSCLC and highlights the usefulness of XO as a therapeutic target for NSCLC.

The patterns of communication among different chondrocyte subtypes in human cartilage degeneration and regeneration help us understand the microenvironment of osteoarthritis and optimize cell-targeted therapies. Here, a single-cell transcriptome dataset of chondrocytes is used to explore the synergistic and communicative patterns of different chondrocyte subtypes. We collected 1600 chondrocytes from 10 patients with osteoarthritis and analyzed the active communication patterns for the first time based on network analysis and pattern recognition at the single-cell level. Manifold learning and quantitative contrasts were performed to analyze conserved and specific communication pathways. We found that ProCs (Proliferative chondrocytes), ECs (Effector chondrocytes), preHTCs (Prehypertrophic chondrocytes), HTCs (Hypertrophic chondrocytes), and FCs (Fibrocartilage chondrocytes) are more active in incoming and outgoing signaling patterns, which is consistent with studies on their close functional cooperation. Among them, preHTCs play multiple roles in chondrocyte communication, and ProCs and preHTCs have many overlapping pathways. These two subtypes are the most active among all chondrocyte subtypes. Interestingly, ECs and FCs are a pair of "mutually exclusive" subtypes, of which ECs are predominant in incoming patterns and FCs in outgoing patterns. The active signaling pathways of ECs and FCs largely do not overlap. COLLAGEN and LAMININ are the main pivotal pathways, which means they are very important in the repair and expansion of joint homeostasis. Notably, only preHTCs assume multiple roles (including sender, receiver, mediator, and influencer) and are involved in multiple communication pathways. We have examined their communication patterns from the perspective of cellular interactions, revealed the relationships among different chondrocyte subtypes, and, in particular, identified a number of active subtypes and pathways that are important for targeted therapy in the osteoarthritic microenvironment. Our findings provide a new research paradigm and new insights into understanding chondrocyte activity patterns in the osteoarthritic microenvironment.

目的 研究三磷酸腺苷结合盒转运子A1(ABCA1)R219K基因多态性在2型糖尿病(T2DM)患者中的分布情况,探讨其与2型糖尿病患者血脂的关系.方法 选取2015年12月～2017年4月期间深圳市龙岗区人民医院收治的85例T2DM患者和90例健康体检对照组纳入研究,对两组研究对象进行血液标本采集,采用聚合酶链反应(PCR)-限制性片段长度多态性(RFLP)检测ABCA1 R219K等位基因和基因型频率情况,对比两组患者血脂水平以及研究组不同基因型患者血脂水平情况,进一步探讨T2DM患者ABCA1 R219K基因多态性及其与血脂水平的关系.结果 两组研究对象TC,TG,HDL-C和LDL-C水平对比差异均无统计学意义(t=0.191,0.853,0.755和0.597,均P>0.05).两组研究对象ABCA1 R219K等位基因和基因型频率对比差异均无统计学意义(t=0.860,2.393,均P>0.05).ABCA1 R219K等位基因三种基因型之间TC,TG和LDL-C水平差异无统计学意义(F=0.02,0.14,2.00和0.06,P>0.05);KK基因型HDL-C水平明显高于RR和RK基因型,差异有统计学意义(t=2.543和2.630,P<0.05);RR和RK基因型之间HDL-C水平差异无统计学意义(t=0.238,P>0.05).结论 ABCA1 R219K基因多态性并不是T2DM发病的遗传学标志,但其变异参与了T2DM患者脂代谢异常的调节.

目的 探究ABCA1基因功能性SNP对糖尿病患者胰岛β细胞功能的调控作用.方法 选取我院2015年12月-2017年4月收治的糖尿病患者332例,按胆固醇情况分为对照组和实验组,检测各生化指标并进行DNA提取和PCR测定,比较生化指标差异及其相关性,比较各功能性SNP基因频率.结果 TG、TC、HDL-C、INS、CP是与胰岛β细胞功能有关的独立影响因素,多态性位点rs2020927基因频率在对照组和实验组之间存在显著差异(P<0.05),而等位基因位点在两组之间无明显差异(P>0.05).结论 ABCA1基因多态性位点rs2020927可能与糖尿病患者胰岛β细胞功能缺陷有关.

Objective To investigate the value of the expression of Adenosine Triphosphate (ATP) binding cassette transporter A1 (ABCA1),peroxisome proliferator activated receptor γ(PPARγ) and sterol regulato-ry element binding protein (SREBP),adiponectin (ADPN) and liver X recepto α(LXRα) in type 2 diabetic pa-tients.Methods 71 patients with type 2 diabetes received in the hospital from June 2015 to June 2017 were se-lected as the observation group,and 60 healthy persons who underwent the health assessment from June 2015 to June 2017 were selected as the control group.Peripheral venous blood was collected from patients with an empty stomach in the morning,serum was isolated and serum human ADPN content were measured by radio-immunoassay.The levels of serum ABCA1,PPAR γ,SREBP and LXR α were measured by Enzyme linked im-munosorbent assay.Results The serum levels of ABCA1 and ADPN in the observation group were lower than those in the control group,while serum PPARγ SREBP and LXRα levels were higher than those in the control group (P< 0.05);the diagnostic sensitivity and specificity of ABCA 1+ PPARγ+ SREBP+ ADPN + LXRα were higher than those of single detection of ABCA1,PPARγ,SREBP,ADPN and LXRα.Conclusion The levels of ABCA1 and ADPN decreased in patients with type 2 diabetes,while the levels of PPAR γ,SREBP and LXR α was increased.The five joint diagnosis of ABCA1+ PPAR γ+SREBP+ADPN+LXR α has high sensitivity and specificity.It was of important clinical value and worth further application.

目的 了解铅中毒时患儿血液多项元素发生的改变.方法 选取龙岗区人民医院89例确诊为铅中毒的儿童作为血铅异常组,另外选取89例同期该院门诊体检的血铅水平正常儿童作为正常对照组.分别检测2组研究对象的全血微量元素6项(铅、锰、铜、锌、铁、镁)和血清钙水平.结果 血铅异常组患儿铁水平低于正常对照组,锰水平高于正常对照组,差异有统计学意义(P＜0.05).相关性分析显示,血液铅与铁呈负相关关系,差异有统计学意义(r=-0.229,P=0.031).锰与铁、锌与铁、铁与镁呈正相关关系,差异有统计学意义(r=0.506,0.272,0.257;P=0.000,0.010,0.016).结论 铅中毒时,血液铁水平降低而锰水平升高.

目的 检测不同程度铅中毒患儿和对照组患儿铁代谢指标(铁和铁蛋白)水平,分析当铅中毒时,血铅水平是否会引起铁代谢的异常改变.方法 参考1991年美国疾病预防控制中心制定的儿童铅中毒指南将铅中毒儿童分为3组:铅中毒轻度组(100μg/L≤血铅<200μg/L)、铅中毒中度组(200μg/L≤血铅<450μg/L)、铅中毒重度以上组(血铅≥450μg/L).并以同期门诊铅中毒筛查中血铅水平正常的25例儿童作为对照组(血铅<100μg/L).分别检测每组儿童血中铅、铁及铁蛋白水平,并对各组结果进行统计分析比较.结果 血中铁和铁蛋白水平随铅水平升高而呈降低趋势(P<0.001).相关回归分析结果显示,铅与铁和铁蛋白均呈负相关(r=-0.582、-0.569,P<0.001).结论 铅中毒会引起血中铁和铁蛋白水平降低而导致铁代谢异常.

Objective To investigate the diagnostic value of IL-22,IL-27,VEGF,TF and TFPI inhibitor value in the benign and malignant pleural effusions.Method Selected ninety pleural patients were subdivided into two groups according to the results of pathology and iconography diagnosis,ie the benign pleural effusions (benign) group with 43 cases and malignant pleural effusions (malignant) group with 47 cases.To test the diagnostic ability of several indicators used alone and in combination,the concentrations of IL-22,IL-27,VEFG,TF and TFPI were determined by ELISA method,and the concentrations of these five biomarkers in pleural effusion were compared,then receiver operating curve (ROC) analysis was performed.Results A significant difference of IL-22,IL-27,VEGF,TF and TFPI levels were observed in patients with malignant and benign pleural effusion (P＜0.05).Receiver operating curve analysis indicated that several indicators used alone showed a less potent diagnostic ability of benign and malignant pleural effusion,the area under the ROC curve of less than 0.8;while these biomarkers used in combination showed an obvious improvement in sensitivity,specificity and accuracy.Conclusion The combination of several biomarkers can provide a reference for the differential diagnosis of benign and malignant pleural effusion.Because of the high accuracy,efficiency and simplicity,this method is worthy promotion.

Objective To investigate the hematological changes of patients with acquired immunedeifciency syndrome (AIDS) and to explore the inlfuencing factors.Methods Total of 122 patients with AIDS treated in our hospital from January 2013 to December 2014 were collected. The clinical materials, including the results of bone marrow smears and peripheral blood indexes, HIV RNA viral roads and T cell subgroups were analyzed, respectively. All the hematological indexes were compared.Results Among the 122 cases with AIDS, 112 patients (91.80%) were with abnormal peripheral blood, among whom, 47 patients (38.52%) with leukocytopenia, 102 patients (83.61%) with lymphocyte reduction, 89&nbsp;patients (72.95%) with hemoglobin reduction, 40 patients (32.79%) with thrombocytopenia. The results of bone marrow smears showed that 57 patients (46.72%) were with reduced hyperplasia of the nucleated cells, 92 patients (75.41%) with reduced hyperplasia of the megakaryocytes. Megakaryocytes were hardly seen among patients with AIDS. Furthermore, 90 cases (73.78%) were with lower red blood cell ratio. In contrast, the granulocyte ratio was increased in 79 cases (64.76%). As for bone marrow cell morphology, there was no significant abnormality, except for infectious bone marrow signs, increased and enlarged cytoplasmic granules in granulocyte, bubbles and tissue cells. In patients with CD4+ T cells < 10/μl, bone marrow smears showed 8 cases with hemophagocytic phenomenon, 4 cases withPenicillium marneffei infection, 1 case withHistoplasma infection, and 1 case with toxoplasma infection. Among the 6 patients with cells of unknown classiifcation, 1 case was diagnosed as lymphoma cells by histopathological biopsy and immunohistochemistry, 1 case as possibly lymphoma renal metastases as indicated by PET-CT result, 1 case with systemic lymph node enlargement as acute leukemia through morphology and immunological classiifcation. The levels of CD4+ T cell hyperplasia of bone marrow nucleated cells of 51-200/μl group and 0-50/μl group were signiifcantly higher than that of 201-500/μl group (bothP < 0.05).ConclusionsPatients with AIDS were easily to be aflficted by abnormal peripheral blood, peripheral blood was associated with whether HIV RNA load or antiviral therapy, moreover, the hyperplasia degree of the bone marrow was found to be reduced with the reduction of both the CD4+ T cell count and immunological functions, AIDS patients with delined immune function were easily complicated with blood system infection. Peripheral blood and bone marrow method was simple, quick and could relfect bone marrow hematopoietic function intuitively, and findfungi and parasitic infection and tumor cells timely, and had important clinical significance to complications diagnosis of patients with AIDS, timely reminder to strengthen the primary prevention of opportunistic infections.

目的 探讨胃癌患者外周血中中性粒细胞明胶酶相关载脂蛋白(neutrophil gelatinase-associated lipocalin,NGAL)与其侵袭、转移及预后的关系.方法 收集深圳市龙岗区人民医院2011年1月-2012年6月40例胃癌患者和28名健康体检者的血清标本,通过酶联免疫吸附法(ELISA)检测血清NGAL水平.分析血清NGAL水平与胃癌临床病理参数的关系;通过受试者ROC曲线分析确定血清NGAL与癌胚抗原(CEA)诊断胃癌的诊断性能.通过多因素分析,明确血清NGAL水平与胃癌患者预后的关系.结果 胃癌患者血清NGAL水平明显高于健康对照者.血清NGAL水平与Lauren's分型、淋巴结转移、浸润深度、远处转移及TNM分期有关(P＜0.05).NGAL取12.51 ng/ml作为血清诊断胃癌的最佳截点值,其灵敏度为84％,特异度为90％.NGAL阳性和阴性患者生存期中位数分别为12个月和27个月,差异有统计学意义(P＜0.05).结论 血清NGAL水平与胃癌的发生、浸润和转移相关,用于胃癌早期筛查、预后评估有重要的临床应用价值.

Objective To explore the application value of IL‐22 ,IL‐27 ,VEGF ,TF and TFPI in the identification of benign and malignant pleural effusion .Methods The enzyme‐linked immunosorbent assay(ELISA) was adopted to detected the five molecular markers levels of IL‐22 ,IL‐27 ,VEGF ,TF and TFPI levels in 67 cases of benign pleural effusion and 73 cases of malignant pleural effusion .Results The levels of IL‐22 ,IL‐27 ,VEGF ,TF and TFPI in the malignant pleural effusion group was significantly higher than those in the benign pleural effusion group ,and the differences were statistically significant (P<0 .05) .The multi‐factor Logis‐tic aggression analysis results showed that only VEGF was an independent influencing factor for identifying benign and malignant pleural effusion .Conclusion The detection of pleural effusion IL‐22 ,IL‐27 ,VEGF ,TF and TFPI is helpful for clinical diagnosis and treatment of the patients with benign and malignant pleural effusion ,in which VEGF can be regarded as an independent factor influ‐encing the identification of benign and malignant pleural effusion .

Objective To investigate the correlation between serum level of tumor antigen(CEA),neuron specific CYFRA21-1(NSE),and the pathological stage of non small cell lung cancer(NSCLC).Methods 86 patients with NSCLC were studied by clinical,pathological and imaging examination.The serum levels of NSE,CYFRA21-1 and CEA were detected by chemiluminescence immunoassay,and the correlation between them was analyzed.Results serum tumor markers CEA,CYSRA21-1 levels were increased gradually with the increase of NSCLC in patients with T and N,and the difference was statistically significant(P <0.05).And the level of NSE was significantly different between T1 and T3(P <0.05),and the difference was not statistically significant(P > 0.05).By analysis,NSCLC,N,T and CEA were positively correlated with the level of and CYSRA21-1(r=0.56,0.71,P <0.05).Conclusion the preopera-tive serum CEA and CYSRA21-1 levels in patients with NSCLC can be used to determine the pathological staging, which has a high clinical application and wide spread value.

目的：分析原发性胆汁性肝硬化（PBC ）合并自身免疫性疾病患者免疫指标及补体水平变化，探讨其与病情评估及诊断的临床意义。方法收集PBC患者85例作为研究对象，根据是否合并其他免疫自身疾病分为PBC组46例，PBC合并自身免疫疾病组39例。其中PBC合并干燥综合征（SS）8例、甲状腺炎22例、系统性红斑狼疮3例、类风湿关节炎（RA ）6例。并随机抽取健康对照组50例，检测三组免疫指标、补体水平并进行比较，并对PBC合并自身免疫疾病组免疫指标及补体水平与肝功能指标（TBIL）及凝血酶原活动度（PTA）进行相关性分析。结果 PBC组、PBC合并自身免疫疾病组及对照组在免疫球蛋白G（IgG）、免疫球蛋白M（IgM）、免疫球蛋白A（IgA）、CD4＋、CD8＋、自然杀伤细胞（NK）、C3、C4方面比较差异均有统计学意义（P＜0．05），其中PBC合并自身免疫疾病组的IgG、IgM、IgA、CD4＋水平最高，CD8＋、NK、C3、C4水平均为最低。三组CD4＋／CD8＋水平比较，差异无统计学意义（P＞0．05）。对 PBC合并自身免疫疾病组免疫指标及补体水平与 TBIL及 PTA 进行相关性分析，发现IgG、IgM均与TBIL呈正相关（P＜0．05），C3、C4均与TBIL呈负相关（P＜0．05）。IgG、IgM、IgA、CD4＋均与PTA呈负相关（P＜0．05），CD8＋、NK、C3、C4均与PTA呈正相关（P＜0．05）。结论原发性胆汁性肝硬化合并自身免疫疾病患者的免疫指标（IgM、IgG、IgA、CD4＋、CD8＋、NK）和补体（C3、C4）水平在其病程中的变化体现了重要的相关性，联合检测免疫指标、补体，以及其与TBIL及PTA相关分析对于其病情评估和诊断有重要的临床意义。

Objective To explore the clinical application value of the changes in the levels of urine N‐acetyl‐β‐D‐glucosaminidase (NAG) ,retinol binding protein (RBP) ,transferring (TRF) ,and α1‐microglobulin (α1‐MG) for early diagnosis ,forecast and illness monitoring of type 2 diabetes mellitus (T2DM ) .Methods According to test result of urinary albumin excretion rate(UAER) ,83 patients suffering from T2DM were divided into normal UAER group ,early diabetic nephropathy group and clinical diabetic ne‐phropathy group ,meanwhile ,28 healthy persons were selected into the healthy control group ,the levels of NAG ,RBP ,TRF ,and α1‐MG were compared in the four groups .Results The levels of NAG ,RBP ,TRF ,and α1‐MG in the normal UAER group ,early dia‐betic nephropathy group and clinical diabetic nephropathy group were significant higher than the control group ,the differences were significant (P< 0 .05) .The four indicators in the early diabetic nephropathy group and clinical diabetic nephropathy group were sig‐nificant higher than those of the normal UAER group ,their differences were statistical significant (P< 0 .05) .In the clinical diabetic nephropathy group ,the positive rates of the indicators were highest ,with NAG 64 .0% ,RBP 52 .0% ,TRF 72 .0% and α1‐MG 76 .0% .For both the early diabetic nephropathy group and clinical diabetic nephropathy group ,the positive rates in the combined detection of four items were relatively high ,reaching 80 .0% and 96 .0% .Conclusion The combined detection of NAG ,RBP ,TRF , and α1‐MG showed relatively high positive rates ,could be taken as the basis for the early diagnosis of diabetic nephropathy .

目的 探讨三氯乙烯(TCE)药疹样皮炎患者血中微量元素和肝代谢酶基因表达水平改变.方法 采用全自动生化分析仪测定病例组和对照组血清中Ca,Mg,Fe和P等微量元素含量.采用实时荧光定量PCR(real-time quantitative PCR)技术检测外周血中CYP1A2,CYP2E1,CYP3A4和CYP2C9 mRNA表达水平.结果 TCE皮炎患者血清Ca水平下降明显,治疗后有所提高,差异有统计学意义(P＜0.05);血清Fe含量病例组治疗后较治疗前下降明显,差异有统计学意义(P＜0.05);血清P水平病例组治疗前与对照组比较显著下降,治疗后恢复正常,差异有统计学意义(P＜0.05);血清Mg含量变化不大,稍有下降,组间差异无统计学意义(P＞0.05).TCE皮炎患者外周血肝代谢酶CYP1A2,CYP2E1,CYP3A4和CYP2C9 mRNA表达水平均提高,与对照组比较差异有统计学意义(P＜0.05).结论 三氯乙烯可导致体内微量元素含量变动和肝代谢酶基因表达水平改变,提示某些微量元素和肝代谢酶基因可能参与TCE的毒性代谢机制.

目的 研究糖类抗原-125(CA125)、恶性肿瘤特异性生长因子(TSGF)、癌胚抗原(CEA)以及可溶性白细胞介素2受体(SIL-2R)四种血清肿瘤标志物联合检测在卵巢恶性肿瘤诊断中的价值.方法 选取深圳市龙岗区人民医院2010年2月至2013年7月恶性卵巢肿瘤患者50例,良性卵巢肿瘤患者62例,同时选取门诊健康女性60例作为健康组,测定各组人群CA125、TSGF、CEA以及SIL-2R水平,评价四种血清肿瘤标志物的敏感度和特异度.结果 四种血清肿瘤标志物在恶性卵巢肿瘤患者中明显高于良性组和健康人群组,差异有统计学意义(P＜0.01);四种血清肿瘤标志物诊断卵巢恶性肿瘤的敏感性依次为CA 125＞TSGF＞CEA＞SIL-2R,特异性高低依次为SIL-2R＞CEA＞TSGF＞CA125.四种肿瘤标志物进行平行诊断试验中敏感性高达100％,在系列诊断试验中特异性高达98.39％.结论 CA125、TSGF、CEA以及SIL-2R四种血清肿瘤标志物联合检测诊断卵巢恶性肿瘤具有较强的可靠性,临床上值得推广使用.

Objective To investigateand analyze of AIDS virus,hepatitis C virus and syphilis infection in Longgang district of Shenzhen city from 2012 to 2013,and provide the scientific basis for the prevention and intervention of drug addicts.Methods Questionnaire investigation survey of the health condition and HCV, HIV, TP and serological test of the drug users were performed in 2012-2013. Results 227 HCV antibody positive cases, 15 HIV antibody positive cases and 65TP antibody positive cases were detected among 801 drug users.The infection rate of HCV of male(29.58%) was significantly higher than that of female(17.28%)(χ2=16.3, P0.01); male and female HIV infection rates were 1.87% and 2.47%,respectively, without significant differences; male TP infection rate(7.50%) was lower than that of female(13.58%)(χ2=7.90, P0.01).The infection rate of HCV of 41~ years old age group was the highest, accounted for 50.00%, the second was the 31~ years old age group(37.80%).HIV antibodies positive cases were mainly 21~ years old age group(2.67%) and 31~years oldage group(1.22%); TP antibodies positive cases were mainly 31~(15.04%) and 41~(18.75%) years old age group.Co-infection rates of HCV and TP, HCV and HIV, HIV and TP were 3.37%, 0.62%, and 0.12%, respectively. HCV and HIV and TP mixed infection rate was zero. Conclusions In recent years, HIV, HCV and TP infection rates among the drug users in Longgang district of Shenzhen city were high. Health education and effective prevention and treatment interventions should be strengthened toreduce the infection rate among drug users in infectious diseases.