Objective To explore the clinical value of RDW,NLR,T cell subsets and their combined value in the diagnosis of connective tissue disease-associated interstitial lung disease. Methods A total of 86 patients with connective tissue disease admitted to Guangyuan Central Hospital of Sichuan Province from January 2021 to December 2021 were selected and grouped. 46 patients with connective tissue diseaseassociated interstitial lung disease were selected as the CTD-ILD group. The CTD group included 40 patients without interstitial lung disease associated connective tissue disease. The levels of RDW,NLR and T cell subsets were compared between the two groups. Point-biserial correlation was used to analyze the association between RDW,NLR and T cell subsets and the occurrence of interstitial lung disease associated with connective tissue disease. Receiver operating curve（ROC）was drawn to analyze the clinical value of RDW,NLR,T cell subsets（CD4 + and CD8 + ）and their combined value in diagnosis of interstitial lung disease associated with connective tissue disease. Results The levels of RDW and NLR in the CTD-ILD group were significantly higher than those in the CTD group,and the levels of CD4 + and CD8 + were significantly lower than those in the control group,with statistical significance（t=2.630、3.073,3.225、2.846;P＜0.05）. Point-biserial correlation analysis showed that RDW and NLR were positively correlated with interstitial lung disease associated with connective tissue disease,while T cell subsets were negatively correlated with interstitial lung disease associated with connective tissue disease（P＜0.05）. The ROC analysis results showed that the areas under the curve of RDW,NLR,T cell subsets（CD4 + ,CD8 + ）and the combined effect in the diagnosis of connective tissue diseaseassociated interstitial lung disease were 0.658,0.672,0.690,0.673,0.771,respectively. Conclusion RDW,NLR,and T cell subsets have a certain correlation with connective tissue disease-related interstitial lung disease,and the combined diagnosis of connective tissue disease-related interstitial lung disease has high application value.

Objective To investigate the effect of resveratrol on the growth activity of lung cancer cells and its possible mechanism. Methods The lung cancer cell line H292 was stably cultured, and the medium containing resveratrol of different concentrations was added for intervention. CCK-8 test, flow cytometry and cell colony formation test were used to detect the effects of resveratrol on the growth activity of H292 cells. Immunofluorescence and Western blotting were used to detect the effects of resveratrol on the expressions of Akt/mTOR signaling pathway-related proteins in H292 cells. Results（1）CCK-8 results showed that resveratrol significantly inhibited the proliferation of H292 cells（P＜0.05） in a time-and dosedependent manner. At the same time, resveratrol had a significant effect on the cell cycle of H292, which could arrest the cell cycle at G2/M phase.（2） Resveratrol significantly inhibited the colony formation of H292 cells in a dose-dependent manner（P＜0.05）.（3） Western blotting showed that resveratrol induced the accumulation of LC3-Ⅱ in H292 cells in a dose-dependent manner. Immunofluorescence assay showed that resveratrol induced a significant increase of GFP-LC3 in the nucleus and mitochondria.（4） Autophagy inhibitor 3-MA effectively weakened the inhibitory effect of resveratrol on the viability of H292 cells. Lysosomal acidification inhibitor Bafilomycin A1 promoted the accumulation of LC3-Ⅱ which was induced by resveratrol.（5） Resveratrol down-regulated the expressions of p-Akt, p-P70S6K and p-mTOR proteins in H292 cells（P＜0.05）, but had no significant effects on the expressions of Akt, P70S6K and mTOR proteins（P＞0.05）. Conclusion Resveratrol can inhibit the proliferation of lung cancer cells by suppressing the Akt/mTOR signal pathway activity.

目的:探讨供血动脉化疗栓塞联合氩气刀及冷冻治疗中晚期中央型肺鳞癌的疗效、安全性和对肺功能改善影响.方法:161例中晚期中央型肺鳞癌随机分为供血动脉化疗栓塞联合氩气刀及冷冻治疗组(干预组)和单纯全身静脉化疗组(对照组),两组化疗方案均为GP方案(吉西他滨+顺铂).比较两组患者治疗6月后的临床疗效、不良反应发生及肺功能改善情况.结果:随访6个月,干预组和对照组治疗有效率分别为58.62％和36.48％,生存率分别为93.1％(81/87)和79.7％(59/74),两组差异统计学有意义(P<0.05).治疗后两组患者FEV1、FVC指标均有所改善,干预组改善情况更显著(P<0.05).两组患者不良反应的发生情况有所差异,干预组化疗常见副作用发生率较单纯全身静脉化疗组低.结论:供血动脉化疗栓塞联合支气管镜下氩气刀及冷冻治疗中晚期中央型肺鳞癌在近期疗效、肺功能改善及安全性方面皆优于传统化疗,临床应用价值大,值得推广应用.

目的 研究术前CT指标与全胸腔镜(VATS)肺叶切除手术中转开胸之间的关系.方法 选取2016年12月～2019年12月我院871例肺部占位性病变患者为样本进行前瞻性研究,均完成CT检查并进行VATS肺叶切除术,其中中转开胸手术者为28例(3.21％),作为中转组;采用随机数字表法从顺利完成VATS手术的患者中抽取100例作为VATS组,比较两组一般资料、CT检查结果及手术结果,分析术前CT检查对VATS中转开胸的影响和预测价值.结果 VATS组手术时间、术中出血量、引流时间及住院时间均低于中转组,差异有统计学意义(P<0.05);两组术后感染、气胸及肺不张等并发症差异无统计学意义(P>0.05);VATS组CT征象中最大淋巴结短径以及肿瘤浸润和胸膜凹陷征发生率均低于中转组(P<0.05),两组肺野病灶位置、病灶最大径和淋巴结钙化比较差异无统计学意义(P>0.05);Logistics回归分析显示肿瘤浸润是影响VATS中转开胸的独立危险因素(P<0.05);ROC曲线分析示肿瘤浸润、最大淋巴结短径、胸膜凹陷征以及3项CT指标联合预测VATS中转开胸的灵敏度分别为82.14％、67.86％、67.86％和78.57％,特异度分别为89.00％、69.00％、93.00％和93.00％.结论 VATS中转开胸可对患者术后康复造成不良影响,其主要原因为肿瘤浸润或淋巴结粘连导致血管等解剖结构分离难度增加,肿瘤浸润、最大淋巴结短径和胸膜凹陷征等术前CT指标对评估中转开胸风险具有一定参考价值.

目的:探讨linc00675在肺癌组织和细胞中的表达以及其对肺癌细胞迁移、侵袭的影响.方法:采用qRT-PCR检测linc00675在35例肺癌组织和癌旁组织及肺癌细胞株SPCA1、A549、NCI-H446和人肺正常上皮细胞BEAS-2B中的表达.利用细胞转染实验对肺癌细胞SPCA1进行LV-linc00675和siRNA-linc00675及相关阴性对照的转染,采用qRT-PCR检测linc00675的表达水平.采用Transwell实验检测过表达及干扰linc00675表达对SPCA1细胞迁移和侵袭能力的影响.通过Western blot实验检测细胞上皮标志蛋白E-钙黏蛋白(E-cadherin)、间质标志蛋白N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)的表达水平.结果:linc00675在肺癌组织中的表达显著低于癌旁组织,在肺癌细胞株中的表达显著低于人肺正常上皮细胞.转染LV-linc00675可显著增加linc00675在肺癌细胞中的表达,转染siRNA-linc00675可显著降低linc00675在肺癌细胞中的表达.Transwell实验结果显示,过表达linc00675可显著抑制肺癌细胞的迁移和侵袭能力,干扰linc00675表达可显著增强肺癌细胞的迁移和侵袭能力.Western blot实验结果显示,过表达linc00675可显著抑制SPCA1细胞上皮间质转化(EMT)的发生,干扰linc00675表达后SPCA1细胞发生了明显的EMT.结论:linc00675在肺癌细胞中发挥抑癌作用,linc00675可能通过抑制肺癌细胞EMT的发生从而减弱其迁移和侵袭能力,提示linc00675可能成为肺癌治疗的新靶点.

目的:探讨荧光支气管镜活检诊断小细胞肺癌的临床价值.方法:回顾性分析经荧光支气管镜活检诊断的84例小细胞肺癌患者的临床资料,观察小细胞肺癌患者镜下表现、钳取技巧和临床特点.结果:经荧光支气管镜钳检阳性率(97.62％)高于刷检阳性率(90.05％),差异有统计学意义(P<0.05).84例小细胞肺癌的影像学表现为胸腔积液4例、阻塞性肺炎21例、肺周结节或团块29例、纵膈以及肺门团块影45例;病灶位置为左侧44例、右侧40例;镜下分型为混合型6例、管腔外压型10例、管壁浸润型28例、管内增生型40例.结论:小细胞肺癌影像学表现缺乏明显特征,结界、肿块是小细胞肺癌的主要表现;经荧光支气管镜钳检诊断小细胞肺癌阳性率较高,可为临床诊治提供参考依据.

此次呼吸道新型冠状病毒感染,特别是新型冠状病毒肺炎,对我国造成巨大影响.截至2020年2月22日,确诊患者76 392例,死亡2 348例.我们对这些不幸离世同胞表示深切的哀悼!

新型冠状病毒肺炎的诊断不仅要重视病毒核酸检测,还应结合病史、影像学等临床信息综合判断,同时要甄别容易进展为危重症的患者.在治疗上,要根据患者的基础疾病及肺炎进展速度分层、个体化治疗.重症患者需加强呼吸支持、营养支持,部分患者可给予激素治疗.

目的:探讨磁共振扩散加权成像(diffusion weighted magnetic resonance imaging,DW-MRI)对肺癌患者的鉴别诊断效果.方法:前瞻性选取广元市中心医院2015年5月至2018年2月经常规CT检查发现肺结节的68例患者为研究对象,患者于手术或穿刺活检前行常规MRI和DW-MRI检查,以病理学检验结果为诊断金标准,对不同扩散敏感系数(b值)下良性肺结节和恶性肺结节病变与脊髓信号强度百分比(lesion-to-spinal cord ratio,LSR)进行检测,对具有鉴别诊断价值的LSR进行诊断效果分析.结果:68例肺结节患者经手术或穿刺活检病理检查恶性肺结节患者38例(恶性组),良性肺结节患者30例(良性组).在b值为400 s/mm2和600 s/mm2时,恶性组LSR值明显高于良性组(P<0.05);与病理结果相对照,b值为400 s/mm2时的灵敏度为63.16％,特异度为73.33％,准确率为67.65％;b值为600 s/mm2时的灵敏度为71.05％,特异度为66.67％,准确率为69.12％.根据受试者工作特征(receive operating characteristic,ROC)曲线,b值为400 s/mm2时,LSR值67.86％为最佳临界值,曲线下面积(area under the curve,AUC)为0.691,b值为600 s/mm2时,LSR值56.58％为最佳临界值,AUC为0.711.结论:DW-MRI计算LSR值对诊断恶性肺结节有一定的临床价值.

目的:观察丙酸倍氯米松雾化吸入联合孟鲁司特钠对咳嗽变异性哮喘(CVA)患者气道炎症控制及外周血免疫球蛋白4(IgG 4)及细胞间黏附分子-1(ICAM-1)水平影响.方法:采用便利抽样的方法,纳入120例CVA患者为调查对象,按照数字表法将其随机分成对照组和研究组,每组60例.对照组仅给予吸入丙酸倍氯米松混悬液进行治疗,研究组在对照组治疗基础上加用孟鲁司特钠进行治疗,两组均连续治疗12周,比较研究组和对照组中患者治疗成功率和复发率,并分析两组患者治疗前后血清白细胞介素(IL-5、IL-8和IL-10)、外周血免疫球蛋白4(IgG 4)、细胞间黏附分子-1(ICAM-1)水平以及肺功能支气管舒张试验的变化情况.结果:研究组哮喘控制率优于对照组(75.00％/50.00％),差异有统计学意义(x2=4.658,P=0.031);治疗后,研究组患者IL-5、IL-8、IL-10和TNF-α的水平明显低于对照组(P＜0.05);治疗后研究组血清IgG 4水平高于对照组,ICAM-1水平低于对照组,差异均有统计学意义(P＜0.05);治疗后随访3个月,对照组复发11例(18.33％),研究组复发2例(3.33％),两组比较差异有统计学意义(x2 =6.988,P=0.008).结论:丙酸氟替卡松通过与孟鲁司特钠的联合作用,对于治疗咳嗽变异性哮喘效果显著,有效降低炎症因子及ICAM-1水平,并且对提高IgG 4水平也有很大疗效,值得推广和应用.

目的:分析纤维支气管镜(纤支镜)支气管肺泡灌洗液早期诊断肺曲霉病的效能,讨论诊断阈值的选择.方法 :选取92例因肺部感染入住呼吸重症科的患者,开展前瞻性对照研究.患者均于治疗前接受纤支镜支气管肺泡灌洗液采集,按照患者诊断结果,将其分别纳入肺曲霉病组(n=35,确诊、临床诊断肺曲霉病)、对照组(n=57,非肺曲霉病),对比两组患者支气管肺泡灌洗液半乳甘露聚糖(Galactomannan,GM)检测结果,并分析不同标本吸光度与标准吸光度比值(I)在早期诊断肺曲霉病中的参考效能.结果 :肺曲霉病组支气管肺泡灌洗液GM平均吸光度为(1.33±0.29),高于对照组的(0.41±0.08),差异有统计学意义(P<0.05).以I≥0.5为肺曲霉病的诊断阈值,其灵敏度、特异性分别为88.00％(22/25)、82.09％(55/67);以I≥1.0为肺曲霉病的诊断阈值,其灵敏度、特异性分别为68.00％(17/25)、100.00％(67/67).ROC曲线得出,以I≥0.95为肺曲霉病诊断阈值,其灵敏度、特异性分别为92.00％(23/25)、97.04％(65/67),其AUC达0.920.结论 :支气管肺泡灌洗液GM检测能够为肺曲霉病的早期诊断提供可靠参考,以I≥0.95为肺曲霉病的诊断阈值,能够达到最佳诊断效能.

Objective Diagnostic value of diffusion-weighted imaging with MRI in voxel pheromone for pulmonary atelectasis in patients with lung cancer. Methods A total of 177 patients with atelectasis admitted to Guangyuan Central Hospital of Sichuan Province were selected. Among them, 112 patients with pulmonary atelectasis caused by central lung cancer were experimental group, and patients with pulmonary atelectasis caused by pneumonia were 65 control subjects. In both groups, pleural MRI images of central lung cancer and pneumonia were detected by MR pheripherial incoherent motion diffusion weighted imaging. The image-related parameters of the two groups were analyzed. Results The results of diffusion-weighted imaging of MRI in voxels in both groups showed that central lung cancer tissues expressed high signals on T2 WI, while those in obstructed lung tissues had low signals, and their signal intensity was different. Patients are identified and classified. By comparing the parameters of lung lesions in the two groups of patients, the D value and D * value of the experimental group were (0.65 ± 0.18) × 10-3 mm2/s and (19.76 ± 6.24) × 10-3 mm2/s, respectively. The D value and D* value of the group were (0.66 ± 0.15) × 10-3 mm2/s and (20.13 ± 8.31) × 10-3 mm2/s; the data in the experimental group was lower than the control group, but the difference was not statistically significant (P＞ 0.05), f (％) value of the test group and the control group, the difference was statistically significant (t =-5.1751, P ＜ 0.05). Conclusion The diffusion-weighted imaging of incoherent motion in MRI voxels has high clinical diagnostic value for central pulmonary atelectasis.

目的 探讨表皮生长因子受体-酪氨酸激酶抑制药(EGFR-TKI)靶向治疗对是否伴恶性胸腔积液非小细胞肺癌(NSCLC)患者的疗效差异.方法 回顾性分析120例晚期NSCLC患者的病历资料,根据患者是否伴有恶性胸腔积液分为A组66例(伴有胸腔积液)和B组54例(不伴有胸腔积液),两组均采用基础治疗+EGFR-TKI治疗,比较两组患者的疗效差异及治疗前和治疗3个月后的KPS评分、血清癌胚抗原(CEA)、鳞状上皮细胞癌抗原(SCC)、神经元特异性烯醇化酶(NSE)、血管内皮细胞生长因子(VEGF)水平的变化.结果 A组患者的缓解率为50.00％、总有效率为81.82％,均低于B组的70.37％、94.44％,两组比较,差异均有统计学意义(P﹤0.05).治疗前,A组和B组患者的血清CEA、SCC、NSE、VEGF水平比较,差异均无统计学意义(P﹥0.05);治疗后,两组患者的血清CEA、SCC、NSE、VEGF水平均较本组治疗前降低(P﹤0.05),且A组患者的血清CEA、SCC、NSE、VEGF水平均明显高于B组,差异均有统计学意义(P﹤0.01).治疗前、治疗后两组患者的KPS评分比较,差异均有统计学意义(P﹤0.05),但A组患者治疗前后的KPS评分差值明显低于B组患者,差异有统计学意义(P﹤0.01).结论 EGFR-TKI靶向治疗对是否伴恶性胸腔积液NSCLC患者均具有一定的临床效果,但是对不伴恶性胸腔积液的患者效果更好,患者的KPS评分提高的更多.

Objective To investigate the diagnostic value of spiral CT,spectral CT and PET-CT scanning in patients with lung cancer.Methods Spiral CT,spectral CT,PET-CT scanning and serological CEA,CYFRA21-1 and NSE detection were carried out in 61 patients with suspected lung cancer.The diagnostic value of tumor markers and dif-ferent scanning methods were compared.Results Of the 61 patients,43 cases (70.49％) diagnosed with PET-CT,40 cases(59.02 ％) diagnosed with spectral CT CT and 35 cases (59.02 ％) diagnosed with spectral CT.There were differences of diagnosis rate among the three methods.Of 16 patients with lung adenocarcinoma,14 patients were found with spectral CT.13 and 12 patients were found with PET-CT and spiral CT.The sensitivity of PET-CT and spectral CT scan (93.48％ and 86.96％) were higher than that of spiral CT (76.09％,P ＜ 0.05).The accuracy of PET-CT (91.80％) and spectral CT (81.97％) were much higher than that of spiral CT(68.85％,P ＜0.05).PET-CT and spectral CT for mediastinum metastatic lymph node detection rate (88.89％ and 77.78％) were significantly higher than that of spiral CT (61.11％,P ＜ 0.05).The specificity of PET-CT and spectral CT combined tumor markers (86.67％ and 78.57％) were higher than that of spiral CT combined tumor markers (66.67％,P ＜ 0.05).The accuracy of PET-CT and spectral CT combined tumor markers (93.44％ and 88.52％) were higher than that of spiral CT combined tumor markers (78.69％,P ＜ 0.05).The sensitivity of spectral CT and PET-CT combined tumor markers (91.49,93.48) and accuracy (88.52,91.80) had no obvious difference (P ＜ 0.05).Conclusion Spectral CT and PET-CT in the diagnosis of lung cancer have high sensitivity and accuracy.Spectral CT and PET CT in diagnosis of mediastinal metastatic lymph nodes also have obvious effect.spectral CT combined tumor markers and PET-CT combined tumor markers in the diagnosis of lung cancer have similar clinical effects.

Objective To investigate the relationship between the mutation of epidermal growth factor receptor (EGFR) gene and the biological behavior of lung cancer patients with malignant pleural effusion. Method 100 patients with lung cancer, including 43 cases with malignant pleural effusion (study group), and 57 patients without pleural effu-sion (control group), were observed for the EGFR mutation status. Result The overall EGFR mutation rate in the study group was 72.09%, significantly higher than that of the control group at 19.30%(P<0.05);the EGFR mutation rate of pa-tients with high, moderate and low differentiation in study group was 69.23%, 71.43%and 77.78%, which were signifi-cantly higher than those in the control group, respectively (P<0.05);gender, age, smoking status and differentiation de-gree were independent of the EGFR mutation rate in study group (P>0.05);the Kappa value of the EGFR gene test results obtained from pleural effusion and biopsy specimens was 0.466 (P<0.05), indicating a moderate consistency. Conclusion EGFR mutation rate is higher in lung cancer patients with malignant pleural effusion, but is not associated with gender, age , smoking status and differentiation degree.

目的 比较舒利迭气雾剂吸入及氟替卡松气雾剂吸入联合孟鲁司特口服治疗哮喘的效果.方法 将66例支气管哮喘患者随机分为SFC组、FP/M组各33例.SFC组吸入舒利迭气雾剂,每次1吸,2次/d;FP/M组吸入氟替卡松气雾剂50 μg,每次2吸,2次/d,每晚口服孟鲁司特10 mg/次;两组均治疗12周.比较两组治疗后无症状白天、夜间占比及无缓解性药物使用白天、夜间占比,支气管激发试验中使FEV1下降20%时所需乙酰甲胆碱的累积浓度(PC20).检测比较两组FEV1及白天、夜间呼气峰流速(PEF).检测并比较两组诱导痰中的中性粒细胞、嗜酸性粒细胞、巨噬细胞、淋巴细胞和气道上皮细胞水平,诱导痰中的半胱氨酰白三烯(C-LT)、组胺和IL-8水平.比较两组不良反应发生情况.结果 两组治疗后无症状白天、夜间占比及无缓解性药物使用白天、夜间占比均较治疗前提高(P均<0.05);SFC组治疗后无缓解性药物使用夜间占比高于FP/M组(P均<0.05);SFC组治疗后无症状白天、夜间占比及无缓解性药物使用白天占比与FP/M组相比,P均>0.05.两组治疗后PC20均高于治疗前(P均<0.05),但两组治疗后PC20相比,P>0.05.两组治疗后FEV1及白天、夜间PEF均较治疗前提高,P均<0.05.SFC组治疗后白天、夜间PEF均高于FP/M组治疗后,P均<0.05;但两组治疗后FEV1相比,P>0.05.SFC组、FP/M组分别有17、19例完成实验并获得诱导痰标本.SFC组、FP/M组治疗前后及两组治疗后诱导痰中的嗜酸性粒细胞、巨噬细胞、淋巴细胞、气道上皮细胞、组胺和IL-8水平相比,P均>0.05.SFC组治疗后中性粒细胞较治疗前低(P<0.05),但与FP/M组治疗后相比,P均>0.05.FP/M组治疗后C-LT水平较治疗前低(P<0.01),与SFC组治疗后比较P<0.05.SFC组9例(27%)、FP/M10例(30%)出现不良反应,两组不良反应发生率相比,P>0.05.结论 舒利迭气雾剂吸入、氟替卡松气雾剂吸入联合孟鲁司特口服对哮喘患者诱导痰中炎症细胞数量均无明显改善作用,但舒利迭气雾剂吸入在改善哮喘患者PEF和控制临床症状方面效果更优.

Objective To analyze the pathogen and drug resistance of lung cancer patients complicated with pulmonary infection. Methods The sputum specimens of 130 lung cancer patients complicated with pulmonary in-fection were given pathogenic bacteria culture and drug sensitive test, and the results were analyzed. Results There were a total of 221 strains of pathogenic bacteria isolated, mainly including gram-negative bacteria (59. 73%). Path-ogenic bacteria showed different degree of resistance to all kinds of antibacterial drugs, and there existed multiple drug resistance. Conclusion The main pathogen of lung cancer patients complicated with lung infection is gram-neg-ative bacteria, and it shows serious drug resistance and multiple drug resistance. Etiology examination and drug re-sistance monitoring should be strengthened to provide the basis for clinical rational use of antibiotics.

Objective: To improve the interventional treatment of bronchial artery malformation feeding artery embolization intractable hemoptysis better therapeutic effect. Methods: From May 2009 - December 2013 DSA angiography confirmed cases of hemoptysis caused by bronchial artery malformation, 54 cases were randomly divided into treatment group and control group, treatment group gelatin sponge (GS) joint polyvinyl alcohol particles (PVA) and / or coils of 32 patients in the control group was simply GS22 cases. All patients 1d, 1 week, 1 month, 6 months, 1 year follow-up data variance, rank sum test for analysis.Results:Follow-up treatment group were cured 28 cases, effective in three cases, one case effectively. In the control group were cured 14 cases, effective in 5 cases, 2 cases. Treatment group was higher efficiency, lower relapse rate than the control group, the two groups and testP<0.05, the difference was statistically significant.Conclusion: Feeding artery embolization for the treatment of bronchial artery malformation intractable hemoptysis significant effect, method is safe, durable, bleeding quickly, recurrence rate, effective intervention to improve hemoptysis.Effectively improve the success rate of interventional treatment of hemoptysis.

Objective To evaluate the clinical value of CT guided percutaneous lung biopsy.Methods Lung lesions of 153 patients underwent percutaneous lung biopsy by automatic cutting needle biopsy(18G).Results A total of 153 patients were punctured successfully,including 129 cases of cancer(48 cases had consistent pathologic and biopsy results),7 cases of inflammation,7 cases of necrotic tissue,5 cases of tuberculosis and 4 cases of fungus.Thirty-six patients had compilactions,including 32 cases of pneumothorax(20.9%),3 cases of hemoptysis or bloody sputum(1.9%)and 1 case of pleural bleeding.All the patients were discharged after treatment.Conclusion CT-guided percutaneous needle biopsy of lung lesions is safe and effective,with high diagnosis rate,fewer complications and high clinical value.

Objective To explore the prognosis factor of patients with severe pandemic H1N1 influenza.Methods To analysize manifestation and treatment of 6 cases with severe pandemic H1N1 influenza.Results 6 cases with severe pandemic H1N1 influenza recovered through active anti-virus and mechanical ventination treatment.Conclusion Anti-virus and mechanical ventination treatment can improve the prognosis of patients with severe pandemic H1N1 influenza.