[目的]对比运动性肩袖损伤关节镜修复与保守治疗的疗效.[方法]2016年5月-2020年11月治疗100例运动性肩袖损伤患者.依据治疗前医患沟通结果,50例采用手术治疗,50例采用保守治疗.比较两组疗效.[结果]两组患者均顺利完成治疗,无严重并发症.随时间推移,两组QOL、UCLA和Constant-Murley评分,以及外展上举ROM和外旋ROM均显著增加(P＜0.05).治疗前两组间QOL、UCLA和Constant-Murley评分,以及外展上举ROM和外旋ROM的差异均无统计学意义(P＞0.05),但术后8周,3、6个月,手术组QOL、UCLA和Constant-Murley评分,以及外展上举ROM和外旋ROM均显著优于保守组(P＜0.05).[结论]与保守治疗相比,关节镜手术治疗运动性肩袖损伤效果确切,可有效减轻疼痛,改善肩关节功能和生活质量.

OBJECTIVE:To investigate the short-term effectiveness of femoral neck system (FNS) in the treatment of femoral neck fracture.METHODS:The clinical data of 34 patients with femoral neck fracture admitted between January 2019 and April 2020 who met the selection criteria were retrospectively analyzed and divided into group A (19 patients were treated with conventional cannulated screw internal fixation) and group B (15 patients were treated with FNS internal fixation) according to the different methods of internal fixation. There was no significant difference between the two groups in general data such as gender, age, affected side, cause of injury, fracture type, and time from injury to operation ( P>0.05). The operation time, intraoperative blood loss, fluoroscopy frequency, and fracture healing time were recorded and compared between the two groups. X-ray film and CT examinations were performed postoperatively to evaluate fracture reduction and internal fixation, and the shortening of the femoral neck on the affected side was measured compared with that on the healthy side. The Harris score was used to evaluate hip function.RESULTS:There was 1 unsatisfied reduction case in groups A and B respectively, the rest of the patients in both groups were obtained satisfied reduction. There was no significant difference in the quality of reduction between the two groups ( Z=-0.195, P=0.854). There was no significant difference in operation time between the two groups ( t=0.649, P=0.064). The intraoperative blood loss in group A was significantly less than that in group B, and the fluoroscopy frequency was significantly more than that in group B, with significant differences ( P<0.05). Except for 1 case in group A with screw out at 3 months after operation and no obvious callus formation, all fractures in the two groups reached clinical healing, and there was no significant difference in fracture healing time between the two groups ( t=-0.127, P=0.899). There was no necrosis of femoral head in the two groups. At last follow-up, there were 4 cases of femoral neck shortening in group A and 2 cases in group B. The hip function of both groups recovered well, and there was no significant difference in Harris score at last follow-up ( t=0.956, P=0.346).CONCLUSION:The treatment of femoral neck fracture using FNS has less trauma. Compared with cannulated screw internal fixation, it can reduce the intraoperative fluoroscopy frequency and obtain satisfactory short-term effectiveness.

目的 探讨髋关节不稳疾病患者采用限制性假体行髋关节置换治疗的效果和预后.方法 回顾性分析10例髋关节不稳定患者应用限制性假体行关节置换手术,术后随访1～3年,分别从临床评定,影像学评定,康复功能评定三个方面评价患者术后情况.结果 10例患者均获得1～3年随访,所有患者均无脱位,疼痛明显减轻,生活质量有所提高,临床评定采用Harris评分均高于术前,影像学评定术后有良好的骨长入,无假体松动和骨溶解,康复功能评定采用Barthel指数评分均比术前提高.结论 限制性假体在不稳定性髋关节疾病患者关节置换中有较好的疗效.

目的 探索黄芪多糖对去卵巢大鼠骨量和骨代谢以及BMP-2/Smads信号通路的影响.方法 30只雌性SD大鼠进行去卵巢手术或假手术.正常饲养12周后被分为黄芪多糖组(ASNT组)、对照组(CON组)和去卵巢组(OVX组),其中黄芪多糖组每天给予400 mg/kg黄芪多糖治疗.通过骨密度、骨代谢指标、Micro-CT以及WB检测评估ASNT对骨质疏松症大鼠骨量、骨代谢以及BMP-2/Smads信号通路的影响.结果 经过12周治疗,ASNT组大鼠骨密度较OVX组显著增加(P<0.05);ASNT治疗12周可以降低血清ALP和OC水平,增加血钙含量,增加股骨骨密度.经过12周治疗,ASNT组大鼠骨体积分数(BV/TV)、表面积体积分数(BS/TV)、骨小梁厚度(Tb.Th)、骨小梁数目(Tb.N)较OVX组显著增加;而骨小梁间隙(Tb.Sp)较OVX组显著降低,差异比较有统计学意义(P<0.05).WB结果表明OVX大鼠骨组织的BMP-2/Smsds信号通路受到抑制;ASNT可以通过上调BMP-2、p-Smad1和p-Smad5的表达显著激活BMP-2/Smsds信号通路传导.结论 研究表明ASNT对去卵巢大鼠骨密度和骨量具有保护作用,可能和激活BMP-2/Smads信号通路有关.

目的:探讨不同入路内固定术治疗旋后外旋型Ⅳ度踝关节骨折(AF)的疗效.方法:选取2016年1月至2018年3月本院旋后外旋型Ⅳ度AF患者120例,依据随机数字表分为经腓骨入路(TA)组和经腓骨后外侧入路(TPA)组,每组60例.TA组年龄42.50±6.58岁,男女比例为38/22,给予TA内固定术;TPA组年龄42.82±6.61岁,男女比例为36/24,给予TPA内固定术,比较两组手术、术后康复、踝功能、并发症、骨折愈合等情况.结果:TPA组术中出血量和手术、住院、骨折愈合时间明显低于TA组,差异有统计学意义(P<0.001);TPA组骨折愈合、踝功能优良率明显高于TA组,差异有统计学意义(P<0.05);TPA组并发症率明显低于TA组,差异有统计学意义(P<0.05).结论:与经腓骨入路比较,经腓骨后外侧入路内固定术治疗旋后外旋型Ⅳ度踝关节骨折的疗效更佳,有利于促进患者骨折愈合和踝功能恢复,且安全性更高.

目的 研究脂肪干细胞移植对绝经后骨质疏松模型大鼠c-Fos、c-Jun表达的影响.方法 将3月龄雌性Sprague-Dawley(SD)大鼠随机分为3组:假手术组、骨质疏松模型组与脂肪干细胞移植组.假手术组仅摘除大鼠卵巢周围的脂肪组织,骨质疏松组、脂肪干细胞移植组摘除大鼠双侧卵巢组织.造模成功后,脂肪干细胞组给予2×107个脂肪干细胞尾静脉注射,假手术组和骨质疏松组给予等量生理盐水尾静脉注射,每周1次.8周后,检测各组大鼠骨密度、骨形态、骨代谢指标及c-Fos、c-Jun表达.结果 与假手术组比较,骨质疏松组大鼠右侧股骨中骨保护素(OPG)的表达量及血清中骨碱性磷酸酶(BALP)、骨钙素(BGP)、总Ⅰ型胶原氨基端延长肽(PINP)的含量均明显降低(P＜0.05),右侧股骨中破骨细胞分化因子(RANKL)的表达量及血清中抗酒石酸酸性磷酸酶-5b(TRAP-5b)的含量明显增加(P＜0.05);与骨质疏松组比较,脂肪干细胞组大鼠右侧股骨中OPG的表达量及血清中BALP、BGP、PINP的含量均明显增加(P＜0.05),右侧股骨中RANKL的表达量及血清中TRAP-5b的含量明显降低(P＜0.05);与假手术组比较,骨质疏松组大鼠右侧股骨中c-Fos、c-Jun的表达量明显降低(P＜0.05);与骨质疏松组比较,脂肪干细胞组大鼠右侧股骨中c-Fos、c-Jun的表达量明显增加(P＜0.05).结论 脂肪干细胞移植能够改善绝经后骨质疏松大鼠的骨代谢并增加c-Fos、c-Jun的表达.

目的 研究脂肪干细胞移植对绝经后骨质疏松模型大鼠骨密度及骨组织形态学影响.方法 将3个月龄雌性SD大鼠随机分为假手术组(110只)、骨质疏松组(110只)、脂肪干细胞移植组(116只).假手术组摘除卵巢周围的脂肪组织;骨质疏松组、脂肪干细胞移植组摘除双侧卵巢组织;脂肪干细胞移植组给予2×107个脂肪干细胞尾静脉注射、假手术组和骨质疏松组给予等量生理盐水尾静脉注射,每周1次,8周后检测大鼠的骨密度、骨组织形态变化.结果 与假手术组比较,骨质疏松组大鼠股骨的骨密度明显降低(P＜0.05);与骨质疏松组比较,脂肪于细胞移植组大鼠股骨的骨密度明显升高(P＜0.05).与假手术组比较,骨质疏松组大鼠右侧股骨的骨小梁面积百分比、骨小梁厚度明显降低,骨小梁间距明显增加(P＜0.05);与骨质疏松组比较,脂肪干细胞移植组大鼠右侧股骨的骨小梁面积百分比、骨小梁厚度明显增加,骨小梁间距明显降低(P＜0.05);与假手术组比较,骨质疏松组大鼠左侧股骨中矿物质盐的含量明显降低(P＜0.05);与骨质疏松组比较,脂肪干细胞移植组大鼠左侧股骨中矿物质盐的含量明显增加(P＜0.05).结论 脂肪干细胞移植能够改善绝经后骨质疏松大鼠的骨密度及骨形态学指标.

目的 评估携带腺病毒介导的肝细胞生长因子基因(Ad-HGF)的脐血间充质干细胞对烧伤后大鼠血浆炎症因子的影响.方法 利用密度梯度法分离脐血间充质干细胞,并培养传代.将构建的Ad-HGF转染到脐血间充质干细胞(MOI=100 pfu/cell).正常大鼠3只,不作处理;将60只大鼠(雌雄对半)建立深II度烧伤模型并随机分为3组:模型对照组(模型组,n=12)、脐血间充质干细胞治疗组(治疗组,n=24)、Ad-HGF修饰的脐血间充质干细胞治疗组(Ad-HGF治疗组,n=24).将不同药物经尾静脉注射于大鼠体内.于伤后第1、7、14、21天分别检测C反应蛋白(CRP)、血清肿瘤坏死因子(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、集落刺激因子(G-CSF)、白细胞介素-10(IL-10)和白细胞介素-4(IL-4)含量.结果 烧伤后第1天,3组大鼠促炎因子(CRP、TNF-α、IL-1和IL-6)和抗炎因子(G-CSF、IL-10及IL-4)水平差异均无统计学意义(P均>0.05);在烧伤后第7、14、21天,治疗组和Ad-HGF治疗组大鼠促炎因子(CRP、TNF-α、IL-1和IL-6)和抗炎因子(G-CSF、IL-10及IL-4)水平均低于模型组,治疗组大鼠促炎因子(CRP、TNF-α、IL-1和IL-6)和抗炎因子(G-CSF、IL-10及IL-4)水平均高于Ad-HGF治疗组(P均<0.01).结论 携带Ad-HGF的脐血间充质干细胞可以调节烧伤后大鼠体内炎症因子的释放,从而减轻炎症反应.

目的 比较外侧“L”形入路与跗骨窦入路治疗跟骨SandersⅡ型、Ⅲ型骨折的效果.方法 将42例跟骨SandersⅡ型、Ⅲ型骨折患者,按手术入路的不同分为外侧“L”形入路组(A组)22例和跗骨窦人路组(B组)20例.对比2组患者手术时间、术中出血量、术后引流量、B(o)hler角、Gissane角,并对患者进行随访,比较2组骨折愈合时间,使用足部Maryland评分系统评价术后1年功能恢复情况.结果 42例患者均获随访,随访时间6～18个月,所有患者均未出现切口感染、皮肤坏死、钢板松动外露等并发症,骨折愈合时间3～6个月.参照美国足踝外科Maryland足评分系统评价术后足部功能,A组22例患者中优14例,良5例,可3例,优良率86.4％;B组20例,优14例,良4例,可2例,优良率90％.结论 与外侧“L”形人路相比,跗骨窦入路治疗跟骨SandersⅡ型、Ⅲ型骨折术后患者足踝功能恢复更好,临床疗效可靠.

目的 研究数字化设计结合3D打印技术辅助手术治疗复杂胫骨平台骨折的效果.方法 回顾性分析20例复杂胫骨平台骨折患者采用数字化设计结合3D打印技术辅助进行手术资料.术前收集复杂胫骨平台骨折病例及常用的胫骨平台钢板的CT数据扫描建立标准件库,利用Mimics软件辅助植入钢板及螺钉,记录手术时间、术中出血量、骨折愈合时间、钢板一次性放置准确率、术后并发症、术前术后胫骨平台内翻角及内外侧后倾角度数、术后膝关节功能Rasmussen评分和末次随访时的纽约特种外科医院膝关节评分(HSS).结果 20例患者随访时间12～30个月(平均17.6个月),骨折均完全愈合,X线影像学平均愈合时间为11.4周,平均手术时间为(84.40±13.21)min,术中平均失血量(350±14.24)mL,钢板一次性放置准确率100％.术后即刻随访X线Ras-mussen评分:优15例,良4例,可1例,优良率为95％;术后12个月随访时膝关节HSS评分:优14例,良4例,可2例,优良率为90％.术后即刻及3、12个月TPA及内外侧平台PA较术前明显恢复,差异均有统计学意义(P＜0.05);术后即刻及3、12个月的胫骨平台TPA及内外侧平台PA之间比较差异均无统计学意义(P＞0.05).外侧切口皮缘部分坏死、感染1例,术后出现腓总神经损伤症状1例.结论 数字化设计结合3D打印技术应用于复杂胫骨平台骨折有助于制订术前计划、术中精确复位,临床效果满意,可实现精准化、个体化治疗.

Objective To investigate the impact of extended target-guided echocardiography (eFATE)protocol in ICU distributive shock patients. Methods A total of 118 patients were randomly divided into the study group(conventional program + eFATE)group and control group (conventional program group). The patients in both group were assessed by the routine methods. Those parameters such as heart rates(HR),mean arterial pressure (MAP), central venous pressure (CVP), cardiac output(CO), ejection fraction (EF),central venous oxygen saturation(ScvO2),APACHE Ⅱ score, lactic acid were measured every 6 hours in the first week,recorded the fluid intake volume at 6h,24h,72h and 1week. Additionally, in the study group,the patients assessed by ultrasound . The fluid intake volume , the goal-directed therapy(GDT)compliance rate,cardiomyopathy rate,ICU Check-in time,ICU mortality and 28-day mortality between the two groups were compared. Results The fluid intake volumes of 6 hours(P=0.027) and 24 hours liquid(P=0.018) in the study group were significantly lower than those in the control group. The GDT compliance rate of the study group was significantly higher than that in the control group(P=0.019),the ICU stay was significantly lower than that of the control group(P=0.005). The 28-day mortality was significantly lower in the study group than in the control group(P=0.039). Conclusion The extended target-guided echocardiographic scheme has significant positive effect on ICU distributive shock patient's capacity assessment,which can reduce the amount of fluid replacement during the clinical treatment, increase the GDT compliance rate and reduce the mortality.

目的:探讨MIPPO技术结合锁骨钢板镜像塑形技术治疗锁骨骨折的手术技巧和疗效.方法:自2014年9月～2015年12月行锁骨手术治疗并随访患者87例,随机分为2组,观察组45例,行MIPPO技术结合钢板镜像塑形技术治疗;对照组42例,行切开复位锁定加压钢板治疗,比较2组的临床疗效及术后不良事件发生率.结果:本组获得了3～9个月随访,观察组的治疗效果优良率显著高于对照组(P<0.05);2组不良事件例数的差异无统计学意义(P>0.05),但观察组各不良事件及总不良事件均少于对照组.结论:MIPPO技术结合钢板镜像塑形技术治疗锁骨骨折的疗效满意,扩大了锁骨MIPPO的适应证,值得推广.

目的:改良Berry间断皮内缝合法在骨科的临床应用,以同时达到降低感染率、减少术后切口瘢痕的形成的目的.方法:采用改良Berry间断缝合法缝合的178例患者,与以皮内缝合法缝合的142例患者比较,观察2组患者缝合时间、创口愈合情况、术后瘢痕大小及术后瘢痕评分.结果:改良Berry间断缝合法患者手术缝合时间长于对照组,差异有统计学意义(P<0.01);创口感染发生率低于对照组,差异均有统计学意义(P<0.05);患者术后瘢痕无明显差异,差异无统计学意义(P>0.01);术后瘢痕评分无明显差异,差异无统计学意义(P>0.01).结论:改良Berry间断皮内缝合法切口对合整齐,瘢痕小,伤口美观,无线头外露风险,利于伤口引流,降低感染率,值得临床推广应用.

Objective To study the clinical effect of arthroscopy in the treatment of knee discoid meniscus injury,and to analyze the influencing factors of postoperative pain. Methods The clinical data of 200 patients with knee discoid meniscus injury who underwent orthopedic treatment from Jan. 2013 to Aug. 2016 in Ningxia People's Hospital were retrospectively analyzed. There were 86 males and 114 females aged 12 to 76 (mean,45. 24) years, with left knee meniscus injury in 103 cases and right knee meniscus injury in 97 cases,and all meniscus injury types were discoid. Injury degree was based on MRI signal,withⅠdegree injury in 32 cases,Ⅱdegree injury in 67 cases and Ⅲ degree injury in 101 cases. All patients underwent arthroscopic treatment. The treatment effect was observed and they were divided into pain group and painless group according to whether there was pain after surgery. The Ly-sholm knee function score and the clinical data were compared between the two groups,and the factors influencing the postoperative pain were analyzed by single factor and multivariate logistic regression analysis. Results After arthroscopic treatment,the efficacy of the 200 patients were excellent in 119 cases,good in 75 cases,fair in 4 cases and poor in 2 cases,and the excellent rate was 97. 0％. A total of 56 patients after surgery developed knee pain,and the incidence was 28. 0％. The Lysholm score (67. 62 ± 12. 21) in the pain group was significantly lower than that in the painless group (83. 45 ± 13. 57,P<0. 05). The factors influencing postoperative pain were mainly age,artic-ular cartilage injury, premature postoperative weight bearing and no cold compress after operation. Conclusion Clinical efficacy of arthroscopic treatment for knee joint discoid meniscus injury is significant,but postoperative pa-tients are prone to knee pain. Corresponding treatment should be based on related factors.

目的 比较经尺骨鹰嘴截骨入路和经肱三头肌舌形瓣入路手术治疗C型肱骨髁间骨折的疗效.方法 将50例患者随机分成两组,经尺骨鹰嘴截骨术组24例,经肱三头肌舌形瓣术组26例.结果 48例患者随访6～19个月,平均12.8个月,骨折全部愈合,平均愈合时间5.6个月.经尺骨鹰嘴截骨入路术后优良率达91.30％,经肱三头肌舌形瓣入路手术优良率68.00％,差异有统计学意义(P<0.05).结论 经尺骨鹰嘴截骨入路手术治疗C型肱骨髁间骨折的疗效明显优于经肱三头肌舌形瓣人路.

目的 观察不同剂量地塞米松对人骨髓间充质干细胞中脂联素、过氧化物酶增殖物激活受体γ-2(PPARγ-2)的影响,探索糖皮质激素对人骨髓间充质干细胞中脂肪代谢的影响.方法 用人骨髓间充质干细胞,分为空白组、成脂细胞分化组、地塞米松组;将地塞米松组再分为:10-0 mol/L组、10-9 mol/L组、10-8 mol/L组、10-7 mol/L组、10-6 mol/L组、10-5 mol/L组.分别培养1、3、7、9d取材,进行脂联素、PPARγ-2、碱性磷酸酶、甘油三酯的检测.结果 不同浓度地塞米松对人骨髓间充质干细胞具有不同的影响,在10-8 mol/L组、10-7 mol/L组,具有一定的成骨作用.但随着地塞米松浓度的增加,脂联素的表达逐渐减少、PPARγ-2的表达逐渐增多.结论 地塞米松的浓度对脂联素、PPARγ-2的表达具有影响,并影响人骨髓间充质干细胞的分化.

Therapy using acellular spinal cord (ASC) scaffolds seeded with bone marrow stromal cells (BMSCs) has previously been shown to restore function of the damaged spinal cord and improve functional recovery in a rat model of acute hemisected spinal cord injury (SCI). The aim of the present study was to determine whether BMSCs and ASC scaffolds promote the functional recovery of the damaged spinal cord in a rat SCI model through regulation of apoptosis and immune responses. Whether this strategy regulates secondary inflammation, which is characterized by the infiltration of immune cells and inflammatory mediators to the lesion site, in SCI repair was investigated. Basso, Beattie, and Bresnahan scores revealed that treatment with BMSCs seeded into an ASC scaffold led to a significant improvement in motor function recovery compared with treatment with an ASC scaffold alone or untreated controls at 2 and 8 weeks after surgery (P<0.05). Two weeks after transplantation, the BMSCs seeded into an ASC scaffold significantly decreased the number of terminal deoxynucleotidyl transferase dUTP nick end labeling-positive cells, as compared with the ASC scaffold only and control groups. These results suggested that the use of BMSCs decreased the apoptosis of neural cells and thereby limited tissue damage at the lesion site. Notably, the use of BMSCs with an ASC scaffold also decreased the recruitment of macrophages (microglia; P<0.05) and T lymphocytes (P<0.05) around the SCI site, as indicated by immunofluorescent markers. By contrast, there was no inhibition of the inflammatory response in the control and ASC scaffold only groups. BMSCs regulated inflammatory cell recruitment to promote functional recovery. However, there was no significant difference in IgM-positive expression among the three groups (P>0.05). The results of this study demonstrated that BMSCs seeded into ASC scaffolds for repair of spinal cord hemisection defects promoted functional recovery through the early regulation of inflammatory cell recruitment with inhibition of apoptosis and secondary inflammation.

Objective To explore effect of Ad - HGF modified umbilical cord mesenchymal stem cells (UCMSCs)on myocardial injury in burn rats. Methods UCMSCs were collected,cultured and transfected with Ad-HGF. 60 Wistar rats were randomized into 3 groups:UCMSCs treatment(groupA ,n=24),transplantation of Ad-HGF modified UCMSCs (groupB ,n=24)and saline control (group C,n=12). According to different groups,drugs were injected though the tail vein in burn rats. On post burn 12,24,48 and 72 hours,cTnI,CK, CK-MB and BNP were anglyzed. Results At the 12th hour after burn,cTnI,CK,CK-MB and BNP were significantly increased,and by 24th h,CK and CK-MB significantly decreased, after 48th h, cTnI and BNP decreased gradually. During the observation time,the index of B group had statistical significance compared with the other groups. Conclusion Ad-HGF modified UCMSCs has postive effect on the myocardial injury in burn rats,providing a new idea for the clinical job.

Objective To evaluate the clinic effect of Nocling R resorbable medical film for preventing epidural adhesion after laminectomy. Methods A total of 125 laminectomy patients with lumbar disc herniation from June 2012 to December 2013 were enrolled, which included 89 males and 36 females, aged 22-68 years old. All of them were divided into experimental group (n=68, with Nocling R resorbable medical film) and control group(n=57, placement without Nocling R resorbable medical film). The clinic effect in 2 groups after treatment for 3-month was evaluated by Nakai method. Results The curative effect in experimental group was 94.12%, and that in control group was 80.70%. There was statistical significant difference between 2 groups (P<0.05). All of them were no systemic adverse reactions, swelling exudates and sinus formation, and complications as leakage of cerebrospinal fluid and nerve damage. Conclusion It is demonstrated that Nocling R resorbable medical film could effectively reduce epidural adhesion after laminectomy.

目的 评估携带腺病毒介导的人肝细胞生长因子基因(Ad-HGF)的人脐带血干细胞复合软骨脱细胞基质移植对兔膝关节软骨缺损修复后的生物力学影响.方法 利用密度梯度法分离脐血干细胞,培养传代.将构建的Ad-HGF转染脐血干细胞,接种在软骨脱细胞基质,共同培养.建立兔关节软骨缺损模型(32只,共64条膝).随机分为4组:A.Ad-HGF转染脐血干细胞组(n=18条膝);B.Ad-HGF转染脐血干细胞复合脱细胞基质组(n=18条膝);C.脱细胞基质组(n=18条膝);D.空白对照组(n=10条膝).将生长旺盛的Ad-HGF修饰的脐血干细胞(3×106细胞,0.5mL)、接种有Ad-HGF修饰的脐血干细胞(3×106)的软骨脱细胞基质//软骨脱细胞基质和0.5mL生理盐水根据不同组别分别移植于关节软骨缺损处.术后4、8、12周,评价关节活动度,测定再生关节软骨面积以及生物力学.结果 在各观察时间点,B组兔关节肿胀度较轻,且关节活动度明显优于其他组(P＜0.01).术后随着时间推移,软骨缺损处逐渐被新生组织覆盖.12周,B组兔膝关节再生软骨的表面积已基本覆盖造模缺损区,与其余三组比较差异有统计学意义(P＜0.01).各组实验兔的软骨缺损处的剪切强度,弹性模量随着时间的推移,逐渐升高,剪切应变随着时间推移,逐渐降低,B组与其余三组比较差异有统计学意义(P＜0.01).结论 携带HGF基因的人脐带血干细胞复合软骨脱细胞基质移植可以提高兔关节软骨缺损后愈合的质量,缩短愈合时间,为临床软骨损伤的治疗提供新的思路.