Porcine respiratory coronavirus (PRCoV), porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), and pseudorabies virus (PRV) are significant viruses causing respiratory diseases in pigs. Sick pigs exhibit similar clinical symptoms such as fever, cough, runny nose, and dyspnea, making it very difficult to accurately differentially diagnose these diseases on site. In this study, a quadruplex one-step reverse-transcription real-time quantitative PCR (RT-qPCR) for the detection of PRCoV, PRRSV, SIV, and PRV was established. The assay showed strong specificity, high sensitivity, and good repeatability. It could detect only PRCoV, PRRSV, SIV, and PRV, without cross-reactions with TGEV, PEDV, PRoV, ASFV, FMDV, PCV2, PDCoV, and CSFV. The limits of detection (LODs) for PRCoV, PRRSV, SIV, and PRV were 129.594, 133.205, 139.791, and 136.600 copies/reaction, respectively. The intra-assay and inter-assay coefficients of variation (CVs) ranged from 0.29% to 1.89%. The established quadruplex RT-qPCR was used to test 4909 clinical specimens, which were collected in Guangxi Province, China, from July 2022 to September 2023. PRCoV, PRRSV, SIV, and PRV showed positivity rates of 1.36%, 10.17%, 4.87%, and 0.84%, respectively. In addition, the previously reported RT-qPCR was also used to test these specimens, and the agreement between these methods was higher than 99.43%. The established quadruplex RT-qPCR can accurately detect these four porcine respiratory viruses simultaneously, providing an accurate and reliable detection technique for clinical diagnosis.

African swine fever (ASF) is a highly contagious and lethal viral disease that causes severe hemorrhagic fever in pigs. It keeps spreading around the world, posing a severe socioeconomic risk and endangering biodiversity and domestic food security. ASF first outbroke in China in 2018, and has spread to most provinces nationwide. Genotypes I and II ASF virus (ASFV) as the etiological pathogens have been found in China. In this study, three pairs of specific primers and probes targeting the ASFV B646L gene, F1055L gene, and E183L gene were designed to detect universal, genotype I, and genotype II strains, respectively. A triplex crystal digital PCR (cdPCR) was established on the basis of optimizing various reaction conditions. The assay demonstrated remarkably sensitive with low limits of detection (LODs) of 5.120, 4.218, 4.588 copies/reaction for B646L, F1055L, and E183L gene, respectively; excellent repeatability with 1.24-2.01% intra-assay coefficients of variation (CVs) and 1.32-2.53% inter-assay CVs; good specificity for only detection of genotypes I and II ASFV, without cross-reactivity with PCV2, PRV, SIV, PRRSV, PEDV, FMDV, and CSFV. The triplex cdPCR was used to test 1,275 clinical samples from Guangxi province of China, and the positivity rates were 5.05, 3.22, and 1.02% for genotype I, genotype II, and co-infection of genotypes I and II, respectively. These 1,275 clinical samples were also detected using a reported reference triplex real-time quantitative PCR (qPCR), and the agreements of detection results between these two methods were more than 98.98%. In conclusion, the developed triplex cdPCR could be used as a rapid, sensitive, and accurate method to detect and differentiate genotypes I and II strains of ASFV.

Polymyxin is considered as one of the “last line of defense” for the treatment of multi-drug resistant bacteria. With the ever-increasing use of polymyxin in recent years, posing a serious risk to public health. In this study, 24 mcr-1-positive APEC isolates were detected from 493 APEC isolates which were isolated and identified from dead and sick poultry in China, and 57 isolates of APEC were selected for whole genome sequencing. The polymyxin MIC of 24 isolates of mcr-1-positive APEC was determined, and the results showed they were all resistant to polymyxin. Besides, 14 isolates of mcr-1-positive APEC plasmids were successfully conjugated to EC600. The whole genome sequencing results showed that the plasmid types related to the spread of mcr-1 gene were IncHI2 (15/24), IncX4 (10/ 24), IncI2 (4/24); two ST types ST93 (9/24), ST349 (4/24) and two serotypes O166:H15 (5/24), O21:H52 (7/24) accounted for a large proportion of APEC sequencing, showing that the isolated APEC have the diversity of ST types and serotypes. Besides, it is worth noting that 2 isolates of APEC resistant to meropenem were found, and they were detected to contain bla and bla genes, respectively. In this study, the epidemiological investigation of the mcr-1 gene was carried out on APEC in some coastal areas of China from 2019 to2020, and the results enriched the transmission data of the APEC isolates carrying the mcr-1 gene in waterfowl.

African swine fever (ASF) is a severe and highly contagious viral disease that affects domestic pigs and wild boars, characterized by a high fever and internal bleeding. The disease is caused by African swine fever virus (ASFV), which is prevalent worldwide and has led to significant economic losses in the global pig industry. In this study, three pairs of specific primers and TaqMan probes were designed for the ASFV B646L, MGF505-2R and I177L genes. After optimizing the reaction conditions of the annealing temperature, primer concentration and probe concentration, triplex crystal digital PCR (cdPCR) and triplex real-time quantitative PCR (qPCR) were developed for the detection and differentiation of the wild-type ASFV strain and the MGF505-2R and/or I177L gene-deleted ASFV strains. The results indicate that both triplex cdPCR and triplex qPCR were highly specific, sensitive and repeatable. The assays could detect only the B646L, MGF505-2R and I177L genes, without cross-reaction with other swine viruses (i.e., PRRSV, CSFV, PCV2, PCV3, PEDV, PDCoV and PRV). The limit of detection (LOD) of triplex cdPCR was 12 copies/reaction, and the LOD of triplex qPCR was 500 copies/reaction. The intra-assay and inter-assay coefficients of variation (CVs) for repeatability and reproducibility were less than 2.7% for triplex cdPCR and less than 1.8% for triplex qPCR. A total of 1510 clinical tissue samples were tested with both methods, and the positivity rates of ASFV were 14.17% (214/1510) with triplex cdPCR and 12.98% (196/1510) with triplex qPCR, with a coincidence rate of 98.81% between the two methods. The positivity rate for the MGF505-2R gene-deleted ASFV strains was 0.33% (5/1510), and no I177L gene-deleted ASFV strain was found. The results indicate that triplex cdPCR and triplex qPCR developed in this study can provide rapid, sensitive and accurate methods for the detection and differentiation of the ASFV B646L, MGF505-2R and I177L genes.

为了解广西部分地区(玉林市、大新县、那坡县、东兴市、靖西市和百色市)猪群中猪链球菌(Streptococcus suis,SS)毒力基因的流行情况和毒力水平,试验采用PCR方法检测分离到的201株(经鉴定猪链球菌2&1/2型24株,猪链球菌7型2株,猪链球菌9型15株,一共41株)猪链球菌的毒力基因orf2、sly、ef和mrp,并通过斑马鱼模型筛选毒力较强的菌株,测定菌株的半数致死剂量(LD50).结果表明:从广西部分地区分离的201株猪链球菌的4种毒力基因携带率从高到低依次为orf2(85.6％,172/201)＞sly(55.2％,111/201)＞ef(33.8％,68/201)＞mrp(26.4％,53/201);6 个市县主要流行的毒力基因不同,玉林市、大新县、那坡县、东兴市、靖西市和百色市分离的猪链球菌携带最多的毒力基因分别为 orf2(100％)、orf2(89.2％)、orf2(91.5％)、ef(44.4％)、orf2(86.7％)、orf2(40.0％);经鉴定分型的41株猪链球菌中,有7株(17.1％,7/41)同时携带了 4种毒力基因,有11株(26.8％,11/41)同时携带了 3种毒力基因,有17株(41.5％,17/41)同时携带了 2种毒力基因;斑马鱼模型筛选出7株致病力较强猪链球菌,LD50 为4.2×107～3.8×108 cfu/mL,且猪链球菌2型菌株毒力和猪链球菌9型菌株毒力差别不大.说明广西部分地区分离的猪链球菌毒力基因携带率较高,各地区流行的毒力基因多样,对斑马鱼有明显的致病性,但毒力不强,未来应当更加关注猪链球菌9型的流行情况和毒力水平.

African swine fever virus (ASFV) was first identified in 1921 and is extensively prevalent around the world nowadays, which has a significant negative impact on the swine industry. In China, genotype II ASFV was first discovered in 2018, and has spread quickly to different provinces in a very short time; genotype I ASFV was first found in 2020, and has been reported in several provinces since then. To establish an accurate method for detection and differentiation of genotypes I and II ASFV, three primers and probes were designed targeting the ASFV B646L gene for different genotypes, the F1055L gene for genotype I, and the E183L gene for genotype II, and a triplex real-time quantitative PCR (qPCR) for differential detection of genotypes I and II ASFV was developed after optimizing the reaction conditions. The assay showed high sensitivity, and the limits of detection (LOD) of the B646L, F1055L, and E183L genes were 399.647 copies/reaction, 374.409 copies/reaction, and 355.083 copies/reaction, respectively; the coefficients of variation (CVs) of the intra-assay and the inter-assay were 0.22-1.88% and 0.16-1.68%, respectively, showing that this method had good repeatability; the assay could detect only ASFV, without cross-reactivity with other swine viruses including PRRSV, PEDV, PDCoV, CSFV, PRV, and PCV2, showing excellent specificity of this method. A total of 3,519 clinical samples from Guangxi province, southern China, were tested by the developed assay, and 8.16% (287/3,519) samples were found to be positive for ASFV, of which 0.17% (6/3,519) samples were positive for genotype I, 7.19% (253/3,519) samples for genotype II, and 0.80% (28/3,519) samples for genotypes I and II. At the same time, these clinical samples were also tested by a previously reported multiplex qPCR, and the agreement between these two methods was more than 99.94%. In summary, the developed triplex qPCR provided a fast, specific and accurate method for detection and differentiation of genotypes I and II ASFV.

为探究广西部分地区猪群中猪链球菌(Streptococcus suis)的流行情况及耐药性,采集广西6个市县共527份猪组织及鼻腔拭子样品,通过选择培养基对样品中的猪链球菌进行分离和PCR鉴定,共分离到猪链球菌201株(38.1％).其中大新县和那坡县猪链球菌分离率最高,分别为64.0％(64/100)和65.4％(70/107),而靖西市截获的越南走私猪中猪链球菌分离率达29.3％(44/150).对分离到的猪链球菌进行血清型分型鉴定,结果显示201株猪链球菌血清型中2(1/2)型24株(11.9％),7型2株(1.0％),9型15株(7.5％),大新县、那坡县和东兴市主要流行2(1/2)型,靖西市的越南走私猪样品主要流行9型.采用纸片扩散法,测定41株广西猪链球菌分离菌株对临床上常见的34种9类抗生素的耐药性,其中有1株猪链球菌只对2类抗生素中度敏感,对7类抗生素不敏感;有12株(29.3％)细菌多重耐药;大多数猪链球菌(65.9％)对大环内酯类不敏感,对万古霉素(87.8％)、硝基呋喃(87.8％)、氟喹诺酮类(82.9％)敏感.由此可见广西地区猪链球菌分离株有较强的耐药性.

African swine fever virus (ASFV) causes African swine fever (ASF), a devastating hemorrhagic disease of domestic pigs and wild boars. Currently, the MGF505R, EP402R (CD2v) and I177L gene-deleted ASFV strains were confirmed to be the ideal vaccine candidate strains. To develop an assay for differentiating the wild-type and gene-deleted ASFV strains, four pairs of specific primers and TaqMan probes targeting the ASFV B646L (p72), I177L, MGF505-2R and EP402R (CD2v) genes were designed. A multiplex real-time qPCR assay for the differential detection of the wild-type and gene-deleted ASFV strains was developed after optimizing the reaction conditions, including the annealing temperature, primer concentration and probe concentration. The results showed that the multiplex real-time qPCR assay can specifically test the ASFV B646L (p72), I177L, MGF505-2R and EP402R (CD2v) genes with a limit of detection (LOD) of 32.1 copies/μL for the B646L (p72) gene, and 3.21 copies/μL for the I177L, MGF505-2R and EP402R (CD2v) genes. However, the assay cannot test for the classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), porcine circovirus type 2 (PCV2), PCV3 and pseudorabies virus (PRV). The assay demonstrated good repeatability and reproducibility with coefficients of variation (CV) less than 1.56% for both the intra- and inter-assay. The assay was used to test 4239 clinical samples, and the results showed that 12.60% (534/4239) samples were positive for ASFV, of which 10 samples lacked the EP402R gene, 6 samples lacked the MGF505-2R gene and 14 samples lacked the EP402R and MGF505-2R genes. The results indicated that the multiplex real-time qPCR developed in this study can provide a rapid, sensitive and specific diagnostic tool for the differential detection of the ASFV B646L, I177L, MGF505-2R and EP402R genes.

Objectives: Polymyxin is considered as one of the 'last lines of defense' for the treatment of multidrug resistant bacteria. Increased use of polymyxin during recent years poses a risk to public health. The purpose of this study was to investigate the carrying situation of the mcr-1 drug-resistance gene in waterfowl in some coastal areas of China from 2019 to 2020. Methods: Fifty-seven isolated avian pathogenic Escherichia coli strains were selected from 493 APEC isolates for whole-genome sequencing. The 24 mcr-1-positive APEC strains were tested for conjugation and genome-wide analysis, including sequence type (ST) analysis, serotype analysis, and drug-resistance gene analysis. Numerous mcr-1-positive E. coli were downloaded from the National Center for Biotechnology Information (NCBI) for comparative genomic analysis. Results: Antimicrobial susceptibility test results showed that 57 APEC isolates were highly resistant to gentamicin, cefotaxime, and ofloxacin, and 24 mcr-1-positive APEC isolates were resistant to polymyxin. Fourteen isolates of mcr-1-positive APEC plasmids were successfully conjugated to EC600. Both ST156 and ST10 were found in high proportions in human and avian sources through genome-wide analysis; it is worth noting that these two isolates of APEC were detected to contain the bla(NDM-1) and bla(NDM-4) genes, respectively. Conclusion: In this study, the epidemiological investigation of the mcr-1 gene was carried out on APEC in some coastal areas of China from 2019 to 2020, and our results have enriched the data on the transmission of APEC isolates carrying the mcr-1 gene in waterfowl. (C) 2022 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.

为了解广西壮族自治区部分地区马属动物携带梨形虫的情况及种类分布,2019-2020年在广西壮族自治区防城港、崇左、百色与河池等地区共采集162份抗凝血,抽提后运用巢式PCR方法扩增18S rRNA基因并进行测序分析,以了解种类情况.结果 显示,防城港、崇左、百色与河池等四地马属动物的马梨形虫平均感染率为26.54％,分别为40.00％、10.76％、44.82％和10.52％;涉及3个种类,分别是马泰勒焦虫、东方泰勒焦虫、瑟氏泰勒焦虫,其中马泰勒焦虫为优势种;此外还有新发人兽共患的病原体Colpodella sp.和Sarcocystis gjerdei.结果 表明,广西壮族自治区4个地区的马属动物都携带梨形虫,且感染率较高,涉及种类较为丰富,应给以足够重视.

兽医实验室中血清抗体的检测主要采用ELISA检测方法,本文结合兽医实验室的实际操作总结对日常ELISA检测中常出现的问题进行探讨,发现ELISA检测中常见问题的影响因素包括样品的处理、实验操作方法、实验仪器设备等.提示在兽医实验室检测中必须严格掌握ELISA操作方法,减少或避免因操作失误而导致的实验失败,本文可为兽医实验室同行提供帮助.

无浆体病是一种常见的动物血液传染病，会严 重危害到动物的健康和生命安全。目前，该病在我国 很多地区，以及不同类型的动物中均有分布。本文以 南宁市石漠化地区为例，通过调查研究的方式，了解 当地山羊无浆体感染现状。结果显示，无浆体感染在 南宁市石漠化地区山羊养殖中存在一定的发生率，不 同的饲养模式下以及不同品种山羊中，在无浆体感染 方面存在一定的差异。

2018年和2019年广西区动物疫病预防控制中心分别对广西南宁、贵港、防城港、柳州、河池、来宾、百色和桂林8个市进行马传染性贫血的抽样调查.重点监测养马场、马术队、马属俱乐部等的马匹及骡等马属动物.两年共采集马血清5050 份、骡血清842 份,经马传染性贫血琼脂凝胶免疫扩散试验检测均为阴性.结果表明,马传染性贫血在以上8个市区发病感染率为0.

为了解广西地区口蹄疫(FMD)、猪瘟(CSF)、猪繁殖与呼吸综合征(PRRS)、猪伪狂犬病(PR)、布鲁氏菌病和小反刍兽疫(PPR)等重大动物疫病的免疫动态、疫情流行风险,按照国家动物疫病定点监测实施方案相关要求,2016—2018年运用ELISA和实时荧光定量PCR方法,对玉林市和百色市20个定点监测场点的猪、牛、羊开展病原学监测和抗体监测.病原监测结果显示:2016—2018年,未在牛羊群中检出FMD、PPR病原;2016年和2017年CSF病原检出率均为0.38％;3年内均有PRRS病原检出,检出率介于1.89％～6.32％.抗体检测结果显示:2016—2018年,猪O型FMD、CSF、PRRS和PR-gB抗体阳性率均在70％以上,A型FMD抗体阳性率逐年上升,NSP抗体和PR-gE抗体阳性率呈波动状变化;牛O型FMD抗体阳性率分别为94.78％、18.26％、71.11％,亚洲I型分别为76.52％、25.22％、27.78％,NSP抗体阳性率在22.61％～41.74％之间波动;仅2016年检出1份牛布鲁氏菌抗体阳性;3年间,羊O型、亚洲I型FMD和PPR免疫抗体阳性率都很低,未达到70％,且呈下降趋势,NSP抗体阳性率在0.95％～7.62％之间波动,未检出羊布鲁氏菌抗体.结果表明,广西玉林市和百色市的猪群O型FMD、CSF、PRRS、PR免疫效果较好,但牛羊群FMD和PPR免疫效果较差,猪群中仍有CSF、PRRS、PR等疫病流行,牛羊群中存在FMD流行的风险,但牛羊布鲁氏菌病得到有效控制.结果提示,应继续加强上述疫病的免疫和监测,针对免疫效果较差、病原流行率偏高的疫病,要重点做好防控和净化.

为了解广西牛、羊群感染梨形虫情况,以及种类分布,从广西南宁、柳州、百色、河池、贺州等地区牛、羊养殖场采集抗凝血638份,抽提血液DNA组,运用套式PCR方法对梨形虫的18S rRNA进行扩增,并抽取一部分阳性样品进行测序,构建系统发育树.结果显示,5个地区梨形虫平均感染为24.76％,其中牛平均感染率为34.30％,羊平均感染为20.19％.南宁、河池、百色等地区牛平均感染率分别为40.00％、43.24％和23.86％;南宁、柳州、河池、百色、贺州等地区羊平均感染率为0、55.34％、11.48％、4.24％和23.40％.序列对比后涉及的种类有双芽巴贝斯虫、牛巴贝斯虫、环形泰勒虫、东方泰勒虫、吕氏泰勒虫,其中东方泰勒虫、吕氏泰勒虫是优势种.获得的序列与系统发育树中所有参考的种类进化树均在同一个分支上.结果表明,广西5个地区牛、羊梨形虫感染率较高,涉及5个种类,在日常监测和防治牛、羊寄生虫病中应引起足够重视.

本文对广西特种经济动物——蛇开展了寄生虫流行情况的调查,以了解蛇的寄生虫种类和感染情况,为诊断和防治蛇类寄生虫病提供参考和指导.本实验运用蠕虫完全解剖法解剖158条蛇,检查、收集寄生虫,结果显示,蛇寄生虫感染率为50.63％,其中30.38％、12.25％、8.14％和4.34％分别感染1、2、3和3种以上寄生虫.各种寄生虫的感染率及平均感染强度分别为裂头蚴22.15％、1.4;华首属线虫(Kalicephalus)17.72％、7.47;蛇棒状线虫(Rhabdia sp.)10.76％、0.42;环宫属吸虫(Encyclometra)5.76％、0.18;蛇蛔虫线虫(Ophidascaris sp.)0.63％、0.082;异双盘属吸虫(Paradistomum sp.)0.68％、0.01;毛细线虫(Capillararia sp.)5.06％、0.46;蛇棘头虫未定种3.16％、0.11;舌形虫(pentastomid) 15.19％、1.03.结果 表明,此次调查发现蛇感染裂头蚴、舌形虫及毛细线虫等人兽共患寄生虫的感染率较高,且感染强度大,此次调查结果对指导养殖户防治寄生虫及公共卫生具有重要的意义.

禽致病性大肠杆菌(APEC)是一种能引起鸡、火鸡和其他鸟类肠外感染的致病性大肠杆菌,可以导致肉鸡气囊炎、败血型全身感染、蜂窝织炎和蛋鸡输卵管炎、腹膜炎.为了了解广西地区禽致病性大肠杆菌的耐药表型以及耐药基因的携带情况,本实验室对2019年从广西分离到的69株APEC采取K-B药敏纸片法进行药敏试验,药敏结果显示,69株APEC对氧氟沙星(56.5％)、恩诺沙星(69.6％)、氟苯尼考(79.7％)、氨苄西林(91.3％)、四环素(98.6％)耐药率较高,而对美罗培南、丁胺卡那霉素、呋喃妥因均不耐药;其中,多重耐药现象严重,对10种抗菌药物以耐4种、5种、6种的情况居多.同时用PCR扩增的方法对其耐药基因,包括碳青霉稀类、β-内酰胺类、氨基糖苷类、黏菌素类、喹诺酮类、四环素类在内的6大类共计17种耐药基因进行了检测.特别值得关注的是,发现了7株携带mcr-1基因的多黏菌素耐药APEC.药敏纸片法检测菌株的耐药表型和耐药基因存在一定关联度.本研究可为养禽场临床用药提供参考,同时为减缓耐药菌传播、降低对人类健康和公共卫生安全威胁提供依据.

为了解广西地区规模种猪场接种口蹄疫O型和猪瘟疫苗的免疫情况以及更好地指导规模种猪场的生产实践工作,广西壮族自治区动物疫病预防控制中心在2016～2018年期间运用ELISA抗体检测试剂盒检测方法,对4200份广西地区各规模种猪场送检的免疫猪只血清样品进行口蹄疫O型和猪瘟免疫抗体水平检测.结果显示:广西规模场口蹄疫O型免疫抗体合格率分别为84.07％,86.00％,92.37％;猪瘟免疫抗体合格率分别为97.36％,99.08％,95.38％,免疫抗体平均合格率均达到农业农村部对猪群的群体抗体免疫效价70％以上的要求.广西规模猪场口蹄疫O型免疫抗体水平在2016～2018年呈持续上升趋势,这说明通过抗体水平持续跟踪监测、不断优化免疫程序、调整疫苗种类,广西规模猪场的口蹄疫得到了良好的控制.而广西规模猪场猪瘟免疫抗体合格率在2017年后有明显下降,这可能与2017年猪瘟退出强制免疫接种有关,但在各年份免疫抗体平均合格率均保持在95％以上,说明广西规模种猪场对猪群的猪瘟免疫管控到位.

为了了解广西地区竹鼠细小病毒的分子生物学特性与遗传变异情况,通过PCR、序列比对分析等方法,对采集到的某竹鼠场腹泻病料进行病原学检查.结果表明,用设计的引物A1/A2从竹鼠血清、内脏中检测到2株竹鼠细小病毒,将其分别命名为1-XQ、3-ZQ.用软件分析,1-XQ、3-ZQ核苷酸同源性为99.9％;与另外2株广西竹鼠细小病毒分离株的核苷酸同源性分别为96.8％～97.5％和96.9％～97.6％;与蝙蝠细小病毒的同源性较高,为98.9％～99.0％;与老鼠细小病毒的同源性为87.3％～87.6％;与牛、鹅、番鸭以及人类等其他种类细小病毒核苷酸同源性较低,为31.8％～41.6％.说明1-XQ、3-ZQ与广西竹鼠分离毒株、中国分离到的蝙蝠毒株亲缘关系最近,属于同一分支.

对常用的兰州兽医研究所和北京世纪元亨生产的两种口蹄疫非结构蛋白3ABC抗体的ELISA检测方法,在检测猪血清抗体时的敏感性进行比较,以期获得可应用于口蹄疫检疫的最佳试剂盒.采用上述两种阻断ELISA试剂盒对170份猪血清样品进行ELISA检测.结果表明,两种试剂盒的阳性检出率相差不大,阳性检出率分别为5.29％和5.88％.本试验表明:兰州兽医研究所和北京世纪元亨生产的口蹄疫非结构蛋白3ABC抗体检测试剂盒检测结果差异不大,均可应用于口蹄疫血清学筛选性试验.