Objective: To evaluate the combined predictive value of immune-inflammatory and tumor markers for lymph node metastasis (LNM) in gastric cancer (GC) patients. Methods: We conducted a retrospective study of 207 GC patients who underwent radical gastrectomy. Based on postoperative histology, patients were categorized into LNM and non-LNM groups. Preoperative serologic levels of markers including carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA199), carbohydrate antigen 72-4 (CA724), Neutrophil-to-Lymphocyte Ratio (NLR), Platelet-to-Lymphocyte Ratio (PLR), Lymphocyte-to-Monocyte Ratio (LMR), Interleukin-6 (IL-6), and C-Reactive Protein (CRP) were collected. A nomogram prediction model was developed using multivariate logistic regression. Internal validation was performed using Bootstrap resampling, and external validation was conducted on an independent cohort of 97 patients. Results: LNM was present in 55 (26.6%) patients in the training cohort. Multivariate analysis identified preoperative levels of CEA (odds ratio [OR]=1.52, P<0.001), CA724 (OR=1.24, P<0.001), NLR (OR=2.86, P<0.001), and IL-6 (OR=1.97, P<0.001) as independent risk factors for LNM. The nomogram model incorporating these four factors demonstrated excellent discrimination, with an area under the curve (AUC) of 0.93. The model significantly outperformed conventional clinicopathologic indicators (P<0.001). Good calibration and clinical utility were confirmed by calibration curves and decision curve analysis, respectively. The model maintained strong predictive performance in both internal (AUC=0.92) and external (AUC=0.91) validation cohorts. Conclusion: The combination of CEA, CA724, NLR, and IL-6 serves as an effective preoperative predictor of LNM in GC. The nomogram model based on these markers provides a reliable, non-invasive tool for individualized risk assessment and treatment planning.

Objective:Comparison of early cholecystectomy (EC) and delayed cholecystectomy (DC) after percutaneous transhepatic gallbladder drainage (PTGD) for over 80-year-old patients with acute cholecystitis.Methods:Clinical data of 297 over-80-year-old patients of with acute cholecystitis undergoing surgery in Shidong Hospital Affiliated to the University of Shanghai for Science and Technology from January 2016 to January 2023 were retrospectively analyzed, including 123 males and 174 females, aged (86.1±5.2) years. There were 176 cases in EC group and 121 in PTGD-DC group. Demographic data and perioperative outcomes were compared between the groups, including gender, age, ASA score, lab test, grades of acute cholecystitis, symptom, time before EC or PTGD, intraoperative blood loss, conversion, respiratory disfunction, gangrenous cholecystitis, abdominal drainage time, postoperative complication, hospital stay, intensive care time.Results:The baseline characteristics were similar between EC and PTGD-DC groups, including demographics, ASA score, grades of acute cholecystitis, white blood cell counting, platelet counting, level of serum procalcitonin, time before EC or PTGD, and percentage of comorbidity. Compared to PTGD-DC group, Patients in DC group experienced more intraoperative blood loss (118±62 vs 32±31ml], longer operative time (135±43 vs 61±31) min], higher incidence of gangrenouscholecystitis [23.2%(41/176) vs 9.9%(12/121)], more respiratory support [16.5%(29/176) vs 11.6%(14/121)], more conversion to open surgery [22.7%(40/176) vs 9.1%(11/121)], longer postoperative abdominal drainage time (9.1±2.6 vs 3.8±2.3d], longer hospitalization (8.2±3.1 vs 6.1±2.2 d], longer intensive care (9.0±0.3 vs 4.6±0.2 h] (all P<0.05). More complications were observed in EC group, such as bile leakage, postoperative bleeding, unscheduled reoperation, bile duct injury (all P<0.05). Conclusion:PTGD and DC could lower the perioperative risk of elderly patients with acute cholecystitis.

Objective: This study aims to investigate the potential role of relaxin, a peptide hormone, in preventing cellulardeterioration and death in gastric carcinoma cells under hypoxic conditions. It explores the effects of recombinantrelaxin 2 (RLXH2) on growth, cell differentiation, invasive potential, and oxidative damage in these cells.Materials and Methods: In this experimental study, the NCI-N87 cell line was cultured under normal conditions andthen subjected to hypoxia using cobalt chloride (CoCl2). The cells were treated with RLXH2, and various assayswere performed to assess cellular deterioration, death, and oxidative stress. Western blot and quantitative real timepolymerase chain reaction (qRT-PCR) were used to measure the expression levels of nuclear factor erythroid 2-relatedfactor 2 (Nrf2) and HO-1, and the translocation of Nrf2 to the nucleus was confirmed through Western blot analysis.Results: This study demonstrates, for the first time, that RLXH2 significantly reduces the formation of reactive oxygenspecies (ROS) and the release of lactate dehydrogenase (LDH) in gastric cancer cells under hypoxic conditions.RLXH2 also enhances the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase(CAT), leading to a decrease in hypoxia-induced oxidative damage. RLXH2 promotes the translocation of Nrf2 to thenucleus, resulting in HO-1 expression.Conclusion: Our findings suggest that RLXH2 plays a significant protective role against hypoxia-induced oxidativedamage in gastric carcinoma cells through the Nrf2/HO-1 signalling pathway. This research contributes to a betterunderstanding of the potential therapeutic applications of RLXH2 in gastric cancer treatment.

Acute mesenteric ischaemia is divided into different clinical entities which are usually considered separately. Here we report a case of acute mesenteric ischaemia complicated with acute anterior myocardial infarction. The clinical picture suggested that non-occlusive mesenteric ischaemia and acute mesenteric arterial thrombosis were both present in this case. Thus, non-occlusive and occlusive ischaemia may coexist in a coordinated and perceptible pattern.

目的 探讨双镜联合同期治疗胆囊结石合并胆总管结石的临床应用效果.方法 选择2019年1月-2022年3月收治的胆囊结石合并胆总管结石患者146例,按照手术类型分为双镜联合同期手术治疗组(n=73)与双镜分期手术治疗组(n=73),对比两组患者的一般资料、白细胞计数(WBC)、总胆红素(TBIL)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、血清淀粉酶(AMS)、白细胞介素-6(1L-6)、C反应蛋白(CRP)、总手术时间、术后疼痛时间、肛门排气期间、平均住院时间、手术费用、耗材费用、总住院费用;术后胰腺炎、胆漏、出血、腹腔感染等指标.结果 两组分别完成69、68例,双镜联合同期手术治疗患者总手术时间[(133.94±21.17)min vs.(158.64±21.22)min,P＜0.001]、术后疼痛时间[(110.6±23.7)h vs.(173.6±14.8)h,P＜0.001]、肛门排气时间[(115.61±33.1)h vs.(164.8±31.8)h,P=0.005]、平均住院时间[12(6～14)]d vs.[14(9～22)]d,P＜0.001｝、较对照组缩短,总住院费用[(35432.10±2784.17)元 vs.(56982.16±4384.54)元,P＜0.001]较对比组降低,差异有统计学意义.两种治疗方式在WBC、TBIL、AST、ALP、AMS、IL-6、CRP、手术费用、耗材费用,术后胰腺炎、胆漏、出血、腹腔感染差异无统计学意义(P＞0.05).结论 采用双镜联合同期治疗胆囊结石合并胆总管结石方式适用范围广,术后恢复快,减少花费,缩短住院时间,临床有一定优势.

Introduction:Mangiferin is a plant antitumor compound with poor water solubility and low bioavailability. In this study, transferrin-modified mangiferin-loaded solid lipid nanoparticles (Tf-modified MGF-SLNs) were prepared to overcome the above defects.Methods:Tf-modified MGF-SLNs were prepared by the emulsification-solvent evaporation method. The physicochemical properties of Tf-MGF-SLNs such as particle size, zeta potential and in vitro drug release were investigated. We also demonstrated the effect of Tf-MGF-SLNs in lung cancer.Results:The mean hydrodynamic diameter of the Tf-MGF-SLNs was 121.8±2.9 nm with a polydispersity index of 0.134±0.03. According to TEM micrographs, Tf-MGF-SLNs are spherical and uniform, and the EE% was found to be 72.5±2.4%. In vitro release, we identified an initial burst effect release, followed by controlled release, in SLNs at both pHs and the Tf-MGF-SLNs drug accumulation release percentages reached over 68% at pH 4.0 and 72% at pH 7.4 in 6 hours, respectively. In vivo studies showed that depending on surface modification, Tf-MGF-SLNs, which suggested that cell internalization was changed and more drugs entered the cells successfully.Discussion:Tf-MGF-SLNs were highly efficient in suppressing the tumor growth in xenograft tumor model. Sustained release of the drug delivery system and Tf-modified MGF-SLNs played a major role. Tf-MGF-SLNs would be a promising formulation for the treatment of lung cancer.

This study was design to investigate the effects of epirubicin combined with interferon-alpha on adverse reactions and regulatory T cells in patients with liver cancer; 78 patients with liver cancer treated during February 2018 to February 2020 were randomly divided into study group and control group, with 39 cases in each group. Patients in the control group were treated with epirubicin while patients in the study group were treated with epirubicin combined with interferon-alpha. Compared with the control group, the incidence of adverse reactions in the study group was significantly lower (p < 0.05). However, the short-term efficacy of the study group was significantly higher than control group (p < 0.05). After chemotherapy, compared to the control group, the levels of CD4(+)/CD8(+) and CD3(+) in the study group were higher (p < 0.05), while the level of CD4(+)/CD25(+) plus regulatory T cells was lower (p < 0.05), though there was no significant difference in NK cells between the control and study group (p < 0.05). Epirubicin combined with interferon-alpha in the treatment of liver cancer can effectively enhance the therapeutic effect, promote the improvement of inflammatory factors and reduce the possibility of a variety of adverse reactions, which can be adopted extensively.

This study was designed to evaluate the anti-cancer effects of bufalin against the human gastric cancer cells and unveil the underlying mechanism. The results showed that bufalin inhibited the proliferation and colony formation of the MGC-803 gastric cancer cells and exhibited an IC50 of 10 μM. These antiproliferative effects were found to be due to the induction of G2/M cell cycle arrest. The G2/M cell cycle arrest was also concomitant with inhibition of cdc2, cdc25 and cyclin B1. Furthermore, bufalin suppressed the epithelial-to-mesenchymal transition, migration, and invasion of the MGC-803 gastric cancer cells. The Western blot analysis revealed that bufalin exerted its effects via deactivation of EK/ERK signaling pathway. Taken together, these results suggest the potential of bufalin as the lead molecule for the development of chemotherapy for gastric cancer.

Colon adenocarcinoma (COAD) is one of the most common types of malignancy and accounts for >3 million deaths worldwide each year. The present study aimed to evaluate the role of notum palmitoleoyl-protein carboxylesterase (NOTUM) in in vivo and in vitro, and to identify the relationship between NOTUM and the apoptosis of COAD. Moreover, the present study aimed to investigate whether NOTUM regulated Fas cell surface death receptor (FAS)-mediated apoptosis was affected by the Wnt signaling pathway. Gene expression profiling interactive analysis (GEPIA) was used to predict the potential function of NOTUM. Western blotting and reverse transcription-quantitative PCR were conducted to detect the protein and mRNA expression levels of NOTUM in different tissues or cell lines. The occurrence and development of COAD was detected after NOTUM knockdown lentivirus administration. The apoptosis of COAD was also observed. SKL2001 was applied to examine whether the role of NOTUM was regulated by Wnt. GEPIA analysis demonstrated that NOTUM expression in COAD tumor tissue was higher compared with in normal tissues. Pair-wise gene correlation analysis identified a potential relationship between NOTUM and Wnt. NOTUM protein and mRNA expression levels in colon carcinoma tissues and RKO cells were increased. NOTUM knockdown lentivirus serves a role in inhibiting COAD development by reducing tumor proliferation, reducing tumor size, and increasing the level of apoptosis in vitro and in vivo. Moreover, NOTUM could increase apoptosis in COAD, which was regulated by FAS, and SKL2001 blocked the progress of apoptosis after NOTUM regulation by NOTUM knockdown lentivirus in vitro and in vivo. Collectively, the present results suggested that NOTUM may be able to regulate the apoptosis of COAD, and that Wnt may be the down-stream target signaling of NOTUM in apoptosis.

目的 探讨改良反穿刺技术用于经自然腔道取出标本(nature orifice specimen extraction surgery,NOSES)的全腹腔镜结直肠切除术的安全性和可行性.方法 回顾性分析2019年1～12月47例全腹腔镜结直肠切除术资料,病灶下缘距齿状线5 cm 5例,病灶位于直肠乙状结肠交界部、乙状结肠42例.肿瘤直径1.0～5.0 cm,平均2.8 cm.使用改良反穿刺技术,在腹腔镜下切开结肠壁,将抵钉座置入结肠,连接杆头端自结肠对系膜缘反向穿出,完成经肛门肠管吻合.结果 47例手术均获得成功,无中转开腹,手术时间40～280 min(平均98 min),抵钉座置入时间2～5 min(平均3.4 min),术后住院时间7～18 d(平均9.8 d).吻合口漏4例,保守治疗治愈,无其他并发症发生.术后随访3～12个月(平均7.9月),无并发症及复发.结论 改良反穿刺技术应用在全腹腔镜结直肠切除术中安全可靠,降低腹腔镜手术难度.

Background Gastric carcinoma (GC) is a ubiquitous malignant tumor worldwide. Circular RNA paired-related homeobox 1 (circ-PRRX1), one kind of non-coding RNAs, has been reported to act as a promoter in tumor growth. This study aims to explore the effects of circ-PRRX1 on proliferation, apoptosis, and metastasis in GC and the underlying regulatory mechanisms. Methods The expression of circ-PRRX1, miR-665, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) mRNA was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot was used to analyze YWHAZ protein expression. 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-Htetrazolium bromide (MTT), flow cytometry, and transwell assay were carried out to assess the viability, apoptosis, migration, and invasion in GC cells. The interaction between miR-665 and circ-PRRX1 or YWHAZ was predicted by StarBase v2.0 and identified by dual-luciferase reporter system. Xenograft mouse model was employed to determine the effects of circ-PRRX1 knockdown on GC growth in vivo. Results Compared with normal tissues and cells, circ-PRRX1 and YWHAZ levels were upregulated, and miR-665 was downregulated in GC tissues and cells. Functionally, circ-PRRX1 knockdown inhibited the viability, migration, and invasion and promoted apoptosis in GC cells, whereas anti-miR-665 abolished these effects. Mechanistically, circ-PRRX1 was confirmed as a sponge of miR-665 to regulate YWHAZ expression. Xenograft mouse model suggested that circ-PRRX1 knockdown reduced GC cells growth in vivo. Conclusion Circ-PRRX1 knockdown suppressed GC development by targeting miR-665 to inhibit YWHAZ expression, and the potential molecular mechanism may provide a theoretical basis for GC therapy.

目的 研究miR 301b 3p在肝细胞癌中的表达情况及其对肝癌细胞增殖的影响.方法 采用qRT-PCR检测miR 301b 3p在肝癌组织和细胞系中的表达情况.采用免疫印迹法和荧光素酶报告子法研究miR 301b 3p与SATB2蛋白的相互作用关系.采用CCK8实验评估细胞的增殖能力.结果 在本研究中,qRT-PCR结果证实miR-301b-3p在HCC组织和肝癌细胞系中下调表达.将miR-301b-3p mimic转染至HepG2细胞后,HepG2细胞的增殖能力显著被抑制.结合生物信息学分析、荧光素酶报告实验、qRT PCR以及免疫印迹实验中证实,SATB2是miR 301b 3p在肝癌细胞中的直接作用靶点.在HepG2细胞中,miR-301b 3p抑制SATB2的mRNA和蛋白表达水平.结论 本研究结果表明,miR-301b-3p通过抑制SATB2调控肝癌细胞增殖,提示miR-301b-3p可能是肝癌治疗的一个潜在的靶点.

OBJECTIVE: In recent years, long non-coding RNAs (lncRNAs) have emerged for regulating the development, as well as progression in colorectal cancer (CRC), which assists in finding new targets for CRC treatment. A previous study indicated that INHBA-AS1 promotes oral squamous cell progression by sponging miR-143-3p. However, the exact function possessed by lncRNA INHBA-AS1 in CRC development remains unclear. PATIENTS AND METHODS: The expression level of INHBA-AS1 in CRC tissues and cell lines was determined by qRT-PCR. The functional role of INHBA-AS1 in CRC was investigated by a series of in vitro assays. RNA immunoprecipitation (RIP), bioinformatics analysis was utilized to explore the potential mechanisms of INHBA-AS1. RESULTS: The present study identified INHBA-AS1 as a kind of lncRNA with high expression in CRC tissues and cells. Functionally, NHBA-AS1 downregulation in CRC cells suppressed CRC cell proliferation as well as colony formability. Mechanistically, INHBA-AS1/miR-422a/AKT1 established the ceRNA network to regulate MMP-2, -7, -9 expressions that participated the modulation of CRC progression. CONCLUSIONS: In summary, LncRNA INHBA-AS1 contributes to CRC progression through AKT1 pathway, and provides a new mechanism to regulate CRC development, as well as a potential target for treating CRC.

目的:观察术后早期肠内免疫营养支持对大肠癌患者营养状态、免疫功能及炎症反应的影响.方法:选取大肠癌患者120例,随机分为对照组和观察组各60例作为研究对象,观察组患者实施术后早期肠内免疫营养支持,对照组患者实施常规肠内营养支持;对比两组患者的营养指标、免疫功能指标和炎症指标.结果:两组治疗后血清转铁蛋白(TFN),白蛋白(Alb)及前白蛋白(PA)水平较治疗前显著升高(P<0.05),观察组治疗后以上指标均高于对照组(P<0.05);两组治疗后血清免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、免疫球蛋白A(IgA)、CD4+、CD4+/CD8+比值较治疗前显著升高(P<0.05),观察组治疗后以上指标均高于对照组(P<0.05),两组治疗后CD8+较治疗前显著降低(P<0.05),观察组治疗后CD8+低于对照组(P<0.05);两组治疗后血清C反应蛋白(CRP)、白细胞介素(IL-6)水平较治疗前显著降低(P<0.05),观察组治疗后以上指标均低于对照组(P<0.05);观察组患者胃肠道并发症发生率明显低于对照组,比较差异有统计学意义(P<0.05).结论:大肠癌患者术后实施早期肠内免疫营养支持能够显著改善术后患者营养状态与免疫功能,减少炎症反应和术后胃肠道并发症发生率.

Objective To observe the clinical efficacy of autologous materials in the repair of non-circumferential bile duct defects in Mirizzi syndrome.Methods The clinical data of 21 cases of Mirizzi syndrome with bile duct defect were analyzed retrospectively,who were treated with different autologous materials.There were 18 cases with type Ⅱ,of whom 15 cases were given gallbladder pedicle flap repair and 3 cases received umbilical vein flap repair.There were 3 cases with type Ⅲ,of whom 2 cases were treated with gallbladder pedicle flap repair and 1 case was given umbilical vein flap repair.The T tubes were placed for 4 to 9 months postoperatively in all cases.Results The operation was successfully performed in all the 21 patients without any death.After operation,the bile leakage occurred in 1 patient of type Ⅲ,who underwent biliary bladder flap repair and the leakage was healed after 2 weeks drainage.All cases were treated with T cholangiography.The patients were followed up for 1 to 6 years,which showed no case with abdominal pain,jaundice or bile duct stricture.Conclusion Repairing the non-circumferential bile duct defects with autologous materials is effective and safe in the patients with Mirizzi syndrome.

Recent studies have shown that miR-494-3p is oncogene and has a central role in many solid tumors; however, the role of miR-494-3p in the progression and prognosis of hepatocellular carcinoma (HCC) remains unknown. In this study, it was found that miR-494-3p was up-regulated in HCC tissues. The high level of miR-494-3p in HCC tumors was correlated with aggressive clinicopathological characteristics and predicted poor prognosis in HCC patients. Functional study demonstrated that miR-494-3p significantly promoted HCC cell metastasis in vitro and vivo. Since phosphoinositide 3-kinase/protein kinase-B (PI3K/AKT) signaling is a basic oncogenic driver in HCC, a potential role of miR-494-3p was explored as well as its target genes in PI3K/AKT activation. Of all the predicted target genes of miR-494-3p, the tumor-suppressor phosphatase and tensin homolog (PTEN) were identified. In conclusion, the data we collected could define an original mechanism of PI3K/AKT hyperactivation and sketch the regulatory role of miR-494-3p in suppressing the expression of PTEN. Therefore, targeting miR-494-3p could provide an effective therapeutic method for the treatment of the disease.

Objective To explore the clinical effect of tension-free repair in the treatment of inguinal hernia in elderly patient.Methods A total of 124 elderly patients with inguinal hernia admitted in our hospital in 2016 were randomly divided into a study group(n=62)and a control group (n=62).The control group was treated with open tension-free inguinal hernia repair,whereas with laparoscopic tension-free inguinal hernia repair in the study group.The operation time,intraoperative blood loss,the postoperative pain relief-time,mean days of hospitalization,postoperative recurrence rate,and complications rates were compared between the two groups.Results The more significant improvements were found in study group versus control group in the intraoperative bleeding volume [(19.9±2.0)ml vs.(36.8±-2.5)ml,t=41.564,P=0.000],in the mean hours of postoperative pain [(22.1 ± 4.2) h vs.(35.3 ± 7.0) h,t =12.732,P =0.000],in mean days of hospitalization [(5.5 ± 1.0)d vs.(9.2±1.9)d,t=13.569,P=0.000],in incidence rate of postoperative recurrence(0.0％ vs.6.5％,x2 =4.133,P=0.042),and in postoperative complications rate(3.2％ vs.12.9％,x2 =3.916,P=0.048).Nevertheless,the operation time was longer in the study group than in the control group[(87.0±5.0)min vs.(55.5±4.2)min,t=-37.984,=0.000],Conclusions As compared with open tension-free repair,the clinical efficacy of laparoscopic tension-free hernia repair is exactly sure in the treatment of inguinal hernia,with shorter postoperative hospitalization time and lower incidence of complications.

AIM To assess the value of combined acoustic radiation force impulse (ARFI) imaging, serological indexes and contrast-enhanced ultrasound (CEUS) in distinguishing between benign and malignant liver lesions. METHODS Patients with liver lesions treated at our hospital were included in this study. The lesions were divided into either a malignant tumor group or a benign tumor group according to pathological or radiological findings. ARFI quantitative detection, serological testing and CEUS quantitative detection were performed and compared. A comparative analysis of the measured indexes was performed between these groups. Receiver operating characteristic (ROC) curves were constructed to compare the diagnostic accuracy of ARFI imaging, serological indexes and CEUS, alone or in different combinations, in identifying benign and malignant liver lesions. RESULTS A total of 112 liver lesions in 43 patients were included, of which 78 were malignant and 34 were benign. Shear wave velocity (SWV) value, serum alpha-fetoprotein (AFP) content and enhancement rate were significantly higher in the malignant tumor group than in the benign tumor group (2.39 ± 1.20 m/s vs 1.50 ± 0.49 m/s, 18.02 ± 5.01 ng/mL vs 15.96 ± 4.33 ng/mL, 2.14 ± 0.21 dB/s vs 2.01 ± 0.31 dB/s; P < 0.05). The ROC curve analysis revealed that the areas under the curves (AUCs) of SWV value alone, AFP content alone, enhancement rate alone, SWV value + AFP content, SWV value + enhancement rate, AFP content + enhancement rate and SWV value + AFP content + enhancement rate were 85.1%, 72.1%, 74.5%, 88.3%, 90.4%, 82.0% and 92.3%, respectively. The AUC of SWV value + AFP content + enhancement rate was higher than those of SWV value + AFP content and SWV value + enhancement rate, and significantly higher than those of any single parameter or the combination of any two of parameters. CONCLUSION The combination of SWV, AFP and enhancement rate had better diagnostic performance in distinguishing between benign and malignant liver lesions than the use of any single parameter or the combination of any two of parameters. It is expected that this would provide a tool for the differential diagnosis of benign and malignant liver lesions.

早期诊断是降低肝癌患者病死率的重要方法[1].目前临床上针对肝肿瘤常用的早期检测方法有常规超声、声学造影(CEUS)、血清学检测等.血清学检测是肝脏恶性肿瘤的重要参考指标[2].超声技术简便、无创,CEUS能动态观察肝脏的血流动力学情况,这2种技术应用比较广泛但主要靠检查者肉眼判断,定性诊断肝脏有无占位性病变,受仪器设备和检查者的主观因素影响较大[3].