Objective:To explore the clinical efficacy of photoselective vaporization of the prostate(PVP) for treatment of benign prostatic hyperplasia(BPH).Methods:The clinical data of 167 cases with BPH were analyzed retrospectively, and divided into the observation group(PVP, n=78) and control group(TURP, n=89) according to the o surgical procedures.The operation time, bladder irrigation time, intubation time, drop in hemoglobin, blood loss, postoperative hospitalization time, complications, and the postoperative 1, 6 and 12 months change value of international prostate symptom score(IPSS), maximum urinary flow rate(Q max), postvoid residua(PVR) and quality of life(QOL)score of the two groups were compared. Results:The bladder irrigation time, intubation time, drop in hemoglobin, blood loss and postoperative hospitalization time were significant lower in observation group than in control group( t=-15.755, -7.458, -24.667, -30.332, -10.328, P<0.05). The operation time and hospitalization expenses in observation group was significantly higher than in control group( t=13.699, 5.179, P<0.05); There were no significant difference in IPSS, Q max, QOL and PVR between the two groups at postoperative1, 6, 12 months( P>0.05). The rate of transurethral resection syndrome, secondary bleeding and temporality dysuresia for observation group were lower than that for control group( χ2=4.517, 4.842, 4.722, P<0.05). Conclusions:PVP is as effective as TURP for the treatment of BPH, and as an novel excellent treatment for the treatment of BPH, it has the advantage of small trauma, faster recovery, less bleeding, shorter hospitalization time and less complications, and it is especially appropriate to the patients of high danger and advanced age.

目的 探讨经尿道选择性绿激光前列腺汽化术(PVP)治疗大体积(80mL以上)良性前列腺增生(BPH)的有效性及安全性.方法 回顾性分析采用PVP治疗的236例BPH患者的临床资料,根据前列腺体积大小分为大体积前列腺组(≥80 mL,观察组)和中、小体积前列腺组(＜80 mL,对照组).对比研究两组患者的手术时间、留置导尿时间、膀胱冲洗时间、血红蛋白降低指数、血Na+降低值、术后住院时间和术后并发症,并对两组患者术后1个月、6个月、12个月时的国际前列腺症状评分(IPSS)、生活质量(QOL)评分、最大尿流率(Qmax)、残余尿量(PVR)进行比较.结果 观察组68例,对照组168例,两组患者术前一般资料比较差异无统计学意义(P＞0.05);观察组手术时间较长,术后血红蛋白降低指数和血Na+降低较多,与对照组比较差异均有统计学意义(t=15.475、2.612、2.099,P＜0.05),两组患者在膀胱冲洗时间、留置导尿时间和术后住院时间比较差异均无统计学意义(t=1.577、1.288、1.758,P＞0.05).两组患者术后1、6、12个月IPSS、QOL、Qmax及PVR比较差异无统计学意义(P＞0.05);观察组患者逆行射精的发生率高于对照组(x=7.773,P＜0.05),两组患者其他术后并发症发生率比较差异无统计学意义(P＞0.05).结论 PVP治疗大体积BPH的有效性及安全性与中、小体积BPH相当,是一种治疗大体积BPH安全有效的新方法,值得临床推广使用.

The aim of the present study was to evaluate the functions of miR-200c in the regulation of tumor growth and metastasis in renal cancer cells, and to investigate the underlying mechanisms. In this study, miR-200c was up- and downregulated in two renal cancer cell lines, namely ACHN and A498, and the proliferation, colony formation, migration and invasion of the cells were measured. The expression levels of various mRNAs and proteins were then analyzed using reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. It was found that miR-200c suppressed proliferation, migration and invasion of the renal cancer cells and, conversely, the inhibition of endogenous miR-200c resulted in increased cell proliferation and metastasis. Furthermore, a luciferase reporter assay revealed that miR-200c directly targeted the 3' untranslated regions of the oncogenes B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) and E2F transcription factor 3 (E2F3) mRNAs, reduced the expression of Bmi-1 and E2F3 and regulated the expression of downstream genes, including E-cadherin, N-cadherin, vimentin, p14 and p16. These results indicate a tumor suppressor role for miR-200c in renal cancer cells via the direct targeting of Bmi-1 and E2F3.

目的：探讨MRI对子宫瘢痕妊娠（cesarean scar pregnancy，CSP）的诊断价值。方法：回顾性分析10例经临床证实的CSP患者MRI检查资料，并与临床病理对照。结果：10例孕囊及瘢痕显示良好，孕囊呈长T1长T2改变，瘢痕组织在T1WI、T2WI上均呈低信号；10例中8例位于子宫体下段前壁瘢痕处，1例位于子宫颈管前壁及宫腔中下段，1例位于子宫下段近宫颈内口右前壁；10例病理证实均为CSP或妊娠组织残留。结论：MRI对CSP的诊断有较高的价值，可为治疗方式的选择及预后评估提供重要依据。

Objective: To investigate medical postgraduates'quality of life ( QOL) and its influencing factors, to provide support for interven-tions.Methods:495 medical postgraduates were surveyed with the World Health Organization Quality of Life-Brie (QOL-B).Results: The scores of physiology, psychology, social relationship and environment domain were (61.40 ±11.91), (58.61 ±12.79), (60.71 ±15.62) and (52.91 ±13.82) respectively.In psychological field of quality of life, the scores of medical postgraduates from the most harmonious family are higher than the others; in physical and psychological field, the scores of the first-grader medical postgraduates were higher than the second-grade, and the scores of the Second-grade medical postgraduates are higher than the Third-grade.In social relationship and environmental field of quality of life, the scores of married postgraduates were higher than the unmarried.In physical, psychological and environmental field, the scores of medical postgraduates from the university of 985, 211 were higher than the others; The higher degree of professional satisfaction, the higher the quality of life score in all field; Logistic regression analysis showed the degree of professional satisfaction and health status influence medical postgraduates'self-evaluation of quality of life.Conclusion: Overall, the QOL of medical postgraduates is good, the degree of profes-sional satisfaction, health status, marriage and grades are the influencing factors of the QOL of medical postgraduates.

Objective To explore depression status in preoperative patients and its influence factors, and provide theoretical basis for psychological intervention. Methods A total of 734 preoperative patients were investigated by self-rating depression scale(SDS). Results Among the 734 patients, there were 558(76.02%) without depression,162(22.07%) of mild depression, 12(1.63%) of moderate depression, and 2(0.27%) of severe depression. The incidence of depression was 24.00%. Single factor analysis showed that gender, age, education level, career, income level,payment ways, smoking, and drug were correlated with depression among preoperative patients(P0.05). Logistic regression analysis showed that gender, age, income level and smoking were the influence factor of depression in preoperative patients. Conclusion The main influence factors of depression for preoperative patients are gender, age, income level and smoking, which should be paid attention to by medical staff and family in order to reduce the incidence of depression.

目的 探讨影响前列腺癌患者生存质量的相关因素.方法 采用世界卫生组织生存质量简表(WHOQOL-BREF)、家庭功能评估量表(APGAP)和抑郁自评量表(SDS)对我院泌尿外科住院的113名前列腺癌患者进行问卷调查.结果 全部113名前列腺癌患者生存质量得分生理、心理、社会关系和环境领域得分分别为(48.57±13.25)、(41.42±11.93)、(52.34±12.65)和(60.63±13.94)分;生理、心理、社会关系、环境领域生存质量得分高收入患者高于低收入患者(F=3.55、4.17、3.99、3.72,P＜0.05),医保、新农合患者高于自费患者(F=5.44、3.94、6.04、6.22,P＜0.05),无尿道压迫症状患者高于有尿道压迫症状患者(t =-2.22、-2.98、-2.03、-2.44,P＜0.05);生理、心理和环境领域生存质量得分高文化程度患者高于低文化程度患者(F=3.16、3.67、8.68,P＜0.05);生理、心理和社会关系领域生存质量得分病程短的患者高于病程长的患者(F=3.92、4.30、3.53,P＜0.05),单纯手术患者高于其他治疗方式的患者(F=10.42、7.37、4.42,P＜0.05);家庭功能障碍越严重,生存质量得分越低(F=6.66、4.02、3.79、1.68,P＜0.05),抑郁状况越严重,生存质量得分越低(F=4.36、6.43、3.45、4.81,P＜0.05);Logistic回归分析显示经济状况、尿道压迫症状、家庭功能和抑郁状况是前列腺癌患者生存质量自我综合评分的影响因素.结论 前列腺患者生存质量的影响因素主要有经济状况、付费方式、文化程度等.家庭功能、抑郁状况与前列腺癌患者生存质量明显相关.应充分利用家庭和社会的力量重视患者的身心健康,以提高前列腺患者的生存质量.

目的 探讨麻风病治愈者社会支持与生存质量的相关性,为提高其生存质量提供理论依据.方法 应用社会支持评定量表(SSRS)和简明健康测量量表SF-36对粤西地区8条麻风康复村共1 56例麻风病治愈者进行问卷调查.结果 典型相关分析结果显示,社会支持与生存质量存在相关关系(第一典型相关系数r=0.428,P＜0.01).客观支持与生理功能呈正相关;社会支持中影响较大的是客观支持与对支持利用度,生存质量中影响较大的是生理功能.对支持的利用度与生理功能呈负相关.结论 麻风病治愈者的社会支持与生存质量存在相关关系,应予以充分的社会支持,增强其自我认可感,从而提高其生存质量.

目的 了解廉江市居民糖尿病认知情况,为糖尿病健康教育提供科学依据.方法 2014年7～8月对廉江市503名居民进行糖尿病认知情况的问卷调查.结果 调查503人,糖尿病知识知晓的161人,总体知晓率为32.01％.男性知晓率高于女性(P＞0.05),文化程度大专及以上居民高于高中及以下居民(P＜0.01).63.62％的居民从未测过血糖,1.39％的居民获取糖尿病知识是通过政府宣传,34.45％的居民不清楚糖尿病是否可根治,41.74％的居民认为糖尿病会严重影响日常生活,超过半数(50.70％)的居民认为戒烟戒酒是预防糖尿病的主要途径.结论 廉江市居民对糖尿病相关知识认知不足.

BACKGROUND:MicroRNA-429 (miR-429), a short noncoding RNA belonging to the miR-200 superfamily, plays a crucial role in tumorigenesis and tumor progression. It also acts as a modulator of epithelial-to-mesenchymal transition, a cell development regulating process that affects tumor development and metastasis. The aim of this study was to investigate the potential role of miR-429 in regulating growth and metastasis of renal cell carcinoma.METHODS:miR-429 expression was stably up-regulated or down-regulated in the renal cell carcinoma ACHN and A498 cell lines, and cell proliferation and metastasis were assessed.RESULTS:miR-429 overexpression inhibited cell proliferation, colony formation, migration, and invasion. Suppression of endogenous miR-429 promoted cell growth and metastasis. miR-429 was shown to directly target the 3' untranslated regions of B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI1) and E2F transcription factor 3 (E2F3) transcripts, regulating their expression, as well as that of the downstream epithelial-to-mesenchymal transition markers E-cadherin, N-cadherin, vimentin, p14, and p16.CONCLUSIONS:These results revealed a tumor suppressive role for miR-429 in renal cell carcinoma through directly targeting BMI1 and E2F3.

Objective:To explore the value of diffusion-weighted imaging (DWI) combined with conventional magnetic resonance imaging (MRI) in diagnostic staging and the decision of treatment method in patients with cervical cancer.Methods:The data of DWl MRI from 42 patients pathologically confirmed with cervical cancer were retrospectively analyzed.The apparent diffusion coeffcient (ADC) and exponential ADC (eADC) values were measured.The ADC and eADC values were compared according to different pathologic types and stages of cervical cancer,and between the cervical cancer and normal cervix.The diagnostic accuracy of DWI combined with conventional MRI for pathologic staging was examined.The ADC and eADC values were compared between radiochemotherapy group and the surgery group.Results:The cervical cancer demonstrated high signal intensity on DWl,whereas the normal cervix showed no obviously abnormal signal intensity.The mean ADC value of 42 patients with cervical cancer was lower than that of normal cervix (P =0.000);the mean ADC value of cervical squamous-cell cancer was lower than that of adenocarcinoma (P =0.036);the mean ADC value of radiochemotherapy group before treatment was lower than that of surgery group (P =0.000),whereas the eADC value was higher than that of surgery group (P =0.000).There were no significant differences in mean ADC and eADC values among different pathologic stages of cervival cancer (all P values ＜ 0.05).The diagnostic accuracy of conventional MRI and DWI combined with conventional MRI for pathologic staging were 92.9％ (39/42) and 95.2％ (40/42),respectively (Kappa =0.903 5 and 0.935 8,respectively;P ＜ 0.01).Conclusion:DWl combined with MRI exerts a high value in diagnostic staging of cervical cancer.The ADC value can be used as reference in selection of therapeutic methods for stage Ⅱ B cervical cancer.

目的 了解硕士研究生医学统计学知识掌握情况及课程教学效果.方法 对广东医学院湛江校区2013级全体在读215名硕士研究生进行问卷调查.结果 调查215人,72.09％在硕士入学前学习过统计学,56.28％没参与过任何研究,88.84％认为十分有必要单独设置统计学软件教学课程,对常用实验设计方法熟悉者不足35％,多数研究生对基本统计方法掌握较好,但对高级统计方法掌握程度较低.36.74％的研究生比较满意医学统计学教学效果,60.93％感觉尚可,2.33％感到不满意.结论 硕士研究生医学统计学教学效果尚可,应进一步优化教学内容和教学方式,注重培养研究生统计设计及统计实践能力,达到更好的教学效果.

目的 了解手术病人术前焦虑现状及其影响因素.方法 2014年5月至2015年2月,采用焦虑自评量表(SAS)对734例术前病人进行问卷调查.结果 734例手术病人术前无焦虑463例(63.08％),轻度焦虑234例(31.88％),中度焦虑33例(4.50％),重度焦虑4例(0.54％),焦虑总发生率36.92％.焦虑的发生与性别[0.63(0.45～0.88)]、年龄[1.63(1.28～2.07)]、职业[2.07(1.26～3.41)]、月收入水平[0.69(0.51～0.93)]等因素相关.结论 手术病人术前存在一定程度的焦虑情绪,且受多方面因素的影响,应引起医护人员及家人的关注,降低其术前焦虑的发生率.

Objective To evaluate the diffusion weighted imaging (DWI) and conventional magnetic reso-nance imaging (MRI) in preoperative staging and diagnosis of endometrial carcinoma. Methods The MRI and DWI data of 38 cases with pathologically confirmed endometrial carcinoma cancer were retrospectively analyzed. Tumor of signal characteristics and invasion range were observed, and different staging of ADC value were measured. Invasive depth and extent were evaluated and compared with pathological findings. Results Endometrial carcinoma was usu-ally isointensity on T1WI, hyperintensity on T2WI and DWI. The average ADC value of endometrial carcinoma was (0.832 ± 0.081) × 10-3 mm2/s, which decreased gradually with the increasing. The differences in ADC value of different stages were statistically different (P<0.01). The accuracy of conventional MRI and DWI combined conventional MRI was 84.21%and 89.47%in assessing pathological staging, with no statistically significant difference (P>0.05). The ac-curacy of conventional MRI and DWI combined conventional MRI was 84.21%and 94.74%in assessing deep myo-metrial invasion, with no statistically significant difference (P>0.05). Conclusion DWI combined MRI has a higher value in assessing preoperative staging and diagnosis of endometrial carcinoma, providing an important basis for the selection of treatment method and prognosis of endometrial carcinoma.

MircroRNA(miRNAs)通过与信使RNA(mRNA)的序列互补来降解耙mRNA并抑制蛋白质翻译,在细胞增殖、分化、发育和凋亡等方面起着重要的作用.许多miRNA可作为原癌基因或抑癌基因,在肿瘤的发生和发展中发挥重要作用.越来越多的研究表明,微miR-135(miR-135)与肿瘤的发生发展密切相关,本文主要对近年来miR-135参与调节肿瘤细胞增殖、凋亡、侵袭、转移、耐药和预后等方面的机制作一综述,期望能对肿瘤的早期诊断、治疗和预后判断有所帮助.

目的 探讨湘西南少数民族地区居民生存质量现状,分析其影响因素.方法 随机抽取湖南省新宁县少数民族地区482名居民,采用世界卫生组织生存质量测定量表(WHOQOL-BREF)进行问卷调查.结果 全部482名居民生存质量得分生理领域(58.42±14.73)分,心理领域(57.37±13.39)分,社会关系领域(60.20±15.05)分,环境领域(52.40±13.92)分;生理、心理、社会关系、环境领域生存质量高文化程度居民高于低文化程度居民(F=23.235、24.222、19.602、16.838,P＜0.05),非农业劳动者高于农业劳动者(t=-3.838、-3.723、-3.139、-3.154,P＜0.05),高收入居民高于低收入居民(F=15.400、15.953、5.988、18.042,P＜0.05),未婚居民高于已婚居民、离婚和丧偶居民(F=15.400、15.953、5.988、18.042,P＜0.05),年龄越大,生存质量得分越低(F=31.191、14.614、12.146、11.211,P＜0.05);社会关系领域生存质量其他少数民族居民高于瑶族居民和汉族居民(F=4.514,P＜0.05);不同性别居民生存质量得分差异无统计学意义(P＞0.05).Logistic回归分析显示年龄、民族、文化程度是少数民族地区居民生存质量自我综合评分的影响因素(P＜0.05).结论 湘西南少数民族地区居民生存质量的影响因素主要有文化程度、民族、收入水平等.应从教育、民族交流、经济发展等方面提高少数民族居民的生存质量.

目的 了解湛江市民的交通意识和违规行为,为湛江交通改革和发展提供科学依据.方法 2014年1～2月随机抽取湛江市霞山区532名市民进行交通安全意识与行为的问卷调查.结果 95.86％的市民对湛江交通状况不满意,73.12％的市民认为湛江交通很混乱;45.86％的市民反映交通违规现象普遍,76.50％的市民承认自己曾有过违规行为.45.49％的市民完全同意实施“禁摩”,27.81％的市民反对实施“禁摩”.结论 湛江市城市交通混乱,交通基础设施不完善,市民交通意识薄弱和违规行为常见,相关部门应扩大宣传,强化市民的交通意识同时从严履行监管职责.

The aim of this study was to investigate the function of miR-183 in renal cancer cells and the mechanisms miR-183 regulates this process. In this study, level of miR-183 in clinical renal cancer specimens was detected by quantitative real-time PCR. miR-183 was up- and down-regulated in two renal cancer cell lines ACHN and A498, respectively, and cell proliferation, Caspase 3/7 activity, colony formation, in vitro migration and invasion were measured; and then the mechanisms of miR-183 regulating was analyzed. We found that miR-183 was up-regulated in renal cancer tissues; inhibition of endogenous miR-183 suppressed in vitro cell proliferation, colony formation, migration, and invasion and stimulated Caspase 3/7 activity; up-regulated miR-183 increased cell growth and metastasis and suppressed Caspase 3/7 activity. We also found that miR-183 directly targeted tumor suppressor, specifically the 3'UTR of three subunits of protein phosphatase 2A (PP2A-C alpha, PP2A-C beta, and PP2A-B56-gamma) transcripts, inhibiting their expression and regulated the downstream regulators p21, p27, MMP2/3/7 and TIMP1/2/3/4. These results revealed the oncogenes role of miR-183 in renal cancer cells via direct targeting protein phosphatase 2A. (C) 2014 Elsevier Inc. All rights reserved.