ObjectiveTo study the significance of changes of platelet 5-hydroxytryptamine (5-HT)in type 2 diabetes patients with hypertension.MethodsWe use the fluorospectrophotometry to measure the levels of platelet 5-HT and plasma 5-hydroxyindoles (5-HIS)in 35 normal controls, 59 patients with type 2 diabetes and 57 diabetes patients with hypertension. Results The level of platelet 5-HT in type 2 diabetes group (514.9±153.7)ng/10~ 9pt was apparently lower than that of ormal controls (618.4±194.1)ng/10~ 9pt (P0.05).When diabetes complicated with hypertension, platelet 5-HT (323.1±180.4)ng/10~ 9pt was significantly lower than that of the type 2 diabetes without group (P0.001) and normal controls(P0.001).The level of platelet 5-HT in the diabetes complicated with cardio-cerebrovascular was lower than that without complications.ConclusionDecreasing of platelet 5-HT was associated with elevated blood pressure and increased the risk factorsof cardio-cerebrovascular complications in diabetes patients with hypertesion.

小儿脑炎是造成小儿神经系统损害的重要原因之一.小儿脑炎进入恢复期后，临床症状虽已好转，但神经系统仍存在轻微损害，听觉功能损害是神经损害表现之一.

目的探讨糖尿病及其心脑血管并发症患者血小板5-羟色胺(5-HT)的变化及意义.方法应用磷光-荧光分光光度法测定43例2型糖尿病的患者,49例2型糖尿病心脑血管并发症患者和35例正常对照组的血小板5-HT 和血浆5-羟吲哚类物质(5-HIS)水平.结果 2型糖尿病组血小板5-HT明显低于正常对照组 (P<0.05),2型糖尿病心脑血管并发症组血小板5-HT明显低于2型糖尿病组(P<0.001)及正常对照组(P<0.001),与收缩压、舒张压呈负相关(P<0.05).结论血小板的激活、释放5-HT可能在2型糖尿病及其心脑血管并发症的发生发展中起着一定作用.

目的探讨神经内分泌疾病的血小板5-羟色胺代谢变化.方法用荧光分光光度法测定了37例急性脑梗塞、10例急性脑出血、48例糖尿病和38例甲状腺功能亢进患者的血小板5-羟色胺和血浆5-羟吲哚乙酸含量,并以37例健康成人的测定值作对照,进行对比分析.结果发现急性脑梗塞血浆5-羟吲哚乙酸显著降低,糖尿病血小板5-羟色胺和血浆5-羟吲哚乙酸降低也很显著,甲亢患者的血浆5-羟吲哚乙酸也显著降低,它们的P值均小于0.05.结论这4种疾病可能存在着单胺类神经介质5-羟色胺代谢的紊乱、血小板活性增高和释放5-羟色胺的增加.

Aim To study the relationship between the visual evoked potential(VEP),brain-stem auditory evoked potential(BAEP),somatosensory evoked potential(SEP),event-related potential(ERP) of type-2 diabetes mellitus(DM group) and their clinical parameters .Methods The Nicolet Viking TV type electromyography(EMG)/evoked potential machine was used for measuring VEP,BAEP,SEP and ERP of 30 type 2 DM patients and 30 normal people with similar age and sex .The relationship between the EP manifestations in DM group and their clinical parameters(including course of disease,blood sugar,plasma lipid,renal function and angiopathy) was analyzed with SPSS software parcel.Results In the DM group, abnormal VEP was found in 20 cases, BAEP in 18,MNSEP in 20 ,PTNSEP in 22, and ERP in 11.Compared with control group,the P100 latency of VEP,the latencies of waveⅠ and Ⅴ and the amplitude of waveⅤ as well as the interpeak latencies of each wave in BAEP, the latencies and wave amplitudes in N9 to P25/P38 of MNSEP and PTNSEP, the latency and amplitude of each wave of ERP were significantly increased (F=5.131- 45.213,t=2.695- 3.472,P< 0.05- 0.01) . Furthermore, the changes of latencies as well as the amplitudes of EP were closely related to the level of blood glucose and the concentration of the blood lipids and the renal function as well as vascular pathological changes.Conclusion The VEP,BAEP,SEP and ERP of DM patients are all abnormality in various extent,which parallel with course of disease.

Objectve:To explore the diagnostic value of brainstem auditory evoked potential(BAEP) towards the functional damage of auditory pathway in children.Methods: BAEP was detected in 30 children with encephalitis recovery stage and in 30 age and sex matched non-encephalitic healthy subjects as a control.The peak latency(PL) and change of wave Ⅰ,Ⅲ,Ⅴ, the interpeak latency(IPL) of wave Ⅰ～Ⅲ,Ⅲ～Ⅴ,Ⅰ～Ⅴ,Ⅴ/Ⅰ were observed.Results:BEAP was abnormal in 16 cases of encephalitis with the abnormal rate being 53.3%,showing normal wave Ⅰ,undifferentiation or disappearance of wave Ⅲ and Ⅲ～Ⅴ.Conclusion:BAEP detection of might be a sensitive method in determining the disfunction of central nervous system in recovery children with encephalitis.It contributes to the detection of the functional damage of auditory pathway in recovery children with encephalitis.

目的通过对2型糖尿病患者进行脑干听觉诱发电位(BAEP)的检测,早期发现2型糖尿病患者听觉通路功能的异常并明确其病变部位、受损程度以及相关因素.方法用NicoletViking TV型肌电图/诱发电位仪对30例2型糖尿病患者(DM组)及年龄和性别与之相匹配的正常人30例(对照组)进行脑干听觉诱发电位检测.应用SPSS统计软件采用t检验、x2检验和直线相关分析的方法比较两组间BAEP各项参数和率的差异,分析DM组BAEP参数的变化与病程、血糖、血脂、肾功能之间的关系.结果(1)DM组中18例(60%)有BAEP异常,表现为波形缺失、波潜伏期延长及波幅下降.(2)与对照组比较,DM组患者V波潜伏期明显延长(P＜0.05),波幅明显下降(P＜0.05);Ⅰ～Ⅲ、Ⅲ～Ⅴ、Ⅰ～Ⅴ波间期明显延长(P＜0.05).(3)DM组患者BAEP各参数的改变与病程、血脂、肾功能密切相关(P＜0.05).结论BAEP是检测2型糖尿病患者听觉传导通路功能的敏感指标,可较早期地诊断并显示听觉外周和中枢神经传导功能障碍的部位和程度.控制高血糖、高血脂,保护肾功能有利于阻止患者的脑干神经功能的异常和病变的发展.

目的通过对2型糖尿病(DM)患者进行刺激正中神经(MN)和胫后神经(PTN)诱发的体感诱发电位(SEP)的检查,了解病人躯体深感觉通路功能状态,早期发现病人的神经病变.方法用Nicolet Viking TV型肌电图/诱发电位仪对30例2型DM患者进行SEP的检测并与正常人对照.应用SPSS统计软件采用t检验、卡方检验及多元逐步回归分析比较两组间SEP各项参数的差异,分析DM组SEP参数变化与血糖、血脂、肾功能之间的关系.结果①DM组MNSEP异常20例(66.7%),PTNSEP异常22例(73.3%),表现为波形缺失、波潜伏期延长及波幅下降.②DM组MNSEP的N9～N20潜伏期、波幅异常(P＜0.05);PTNSEP的N9～P38潜伏期、波幅异常(P＜0.05).③DM组SEP异常与病程、血糖、血脂、肾功能有关(P＜0.05).结论①SEP是检测2型DM神经病变的敏感指标.②控制血糖、血脂、保护肾功能有利于阻止神经病变的发展.

目的: 了解2型糖尿病(2DM)患者的诱发电位(EP),包括视觉(VEP)、脑干听觉(BAEP)、体感(SEP) 和事件相关电位(ERP)的表现.方法: 用Nicolet Viking TV型肌电图/诱发电位仪对30例2 DM病人(DM组)及30例正常人(对照组)进行VEP、BAEP、SEP、ERP检测.比较两组间EP各项参数的差异.结果:DM组VEP异常20例(66.7%),BAE P 异常18例(60%),上肢正中神经SEP(MNSEP)及下肢胫后神经SEP(PTNSEP)异常分别为20例(66. 7%)和22例(73.3%),ERP异常11例(36.7%),表现为波形缺失、波潜伏期(PL)延长及波幅(Am p)下降.DM组VEP P100的PL,BAEP中Ⅰ波PL,Ⅴ波PL及Amp和各波间期(IPL),MNSEP中 N9～P20, PTNSEP中N9～ P38的PL及Amp、ERP中N2、P3的PL及Amp异常与对照组比较差异均有显著意义(P＜0.05或P＜0.001).结论:DM患者VEP、BAEP 、SEP、ERP都有不同程度异常,EP是检测DM神经病变的敏感指标.

目的:了解2型糖尿病患者的视觉诱发电位(VEP)、脑干听觉诱发电位(BAEP)、体感诱发电位(SEP)、事件相关电位(ERP)的表现及其与临床参量的关系.方法:用Nicolet Viking TV型肌电图/诱发电位仪对30例2型糖尿病患者(DM组)及年龄和性别与之匹配的正常人30例(对照组)进行VEP、BAEP、SEP、ERP检测.应用SPSS统计软件分析DM组的诱发电位(EP)表现及其与临床参量(病程、血糖、血脂、肾功能、血管病变)的关系.结果:DM组①VEP异常20例, BAEP 异常18例, MNSEP异常20例,PTNSEP异常22例,ERP异常11例.②VEP的P100潜伏期(PL)明显延长,BAEP的左侧Ⅰ波PL、双侧Ⅴ波PL、波幅(Amp)和各波间期(IPL)明显异常,SEP周围电位N9至皮层电位P25/P38的PL、Amp以及ERP中N2、P3a、P3b的PL、Amp都有明显异常,与对照组比较P＜0.05,P＜0.001.③EP改变与各临床参量有关.结论:糖尿病患者VEP、BAEP、SEP、ERP都有不同程度异常,其异常程度和病程平行.控制血糖、血脂、保护肾功能、防治血管病变有利于阻止神经病变的发展.

目的研究急性脑梗死患者血小板5-羟色胺(5-HT)与血浆5-羟吲哚乙酸(5-HIAA)的含量及其在脑缺血性损伤中的作用.方法用荧光分光仪测定60例早期脑梗死患者和60例正常对照者血小板5-HT与血浆5-HIAA的含量.结果脑梗死组血小板5-HT较对照组显著下降(P<0.01),其中,重症脑梗死组血小板5-HT水平较轻症组明显下降(P<0.05).各组血浆5-HIAA比较无明显差别.结论脑梗死患者早期存在5-HT代谢异常,血小板5-HT释放增加是缺血性脑血管病继发性脑损害的重要因素之一.

目的验证尼莫地平对脑缺血再灌迟发性神经元死亡(DND)是否有防治效果并探讨其机制.方法采用大鼠四血管脑缺血模型,用HE染色后光镜观察及原位末端标记(TUNEL)检测神经元坏死及凋亡,以药物治疗组与生理盐水对照组作比较,并作统计学处理.结果尼莫地平防治组与生理盐水对照组二者的DND(神经元凋亡及坏死)无显著性差异(P>0.05);HE及TUNEL检测形态均无差异.结论单用尼莫地平不能有效防治脑缺血再灌DND.

Pulsinelli四血管闭塞法全脑缺血模型[1]广泛应用于脑缺血再灌流神经损害的研究,但成功率低,稳定性差.参照该法,我们建立并改良了大鼠全脑缺血模型.通过反复实践和摸索,在如何提高模型的成功率,缩短模型制作周期等方面,积累了一些经验和体会.