ABSTRACT Background and Aims The prognosis and severity of hepatitis B virus‐associated acute‐on‐chronic liver failure (HBV‐ACLF) cannot be well‐identified by serum biomarkers. The present study aims to determine the role of serum Golgi protein 73 (GP73) in predicting the prognosis and severity of liver necrotizing inflammation induced by HBV‐ACLF. Methods A total of 427 chronic HBV‐infected patients were included for the present study. Among these patients, 179 patients had chronic hepatitis B (CHB), 96 patients had HBV‐related liver cirrhosis (LC), and 152 patients had HBV‐ACLF. The baseline and dynamic changes in serum GP73 levels were measured and compared in CHB, LC and HBV‐ACLF patients. Results The serum GP73 levels were significantly greater in HBV‐ACLF patients when compared to CHB and LC patients. Furthermore, serum GP73 demonstrated excellent performance in distinguishing HBV‐ACLF from CHB and LC, with an area under the curve of 0.969 and 0.824, respectively. In the logistic regression analysis, a high GP73 level was identified as an independent risk factor associated with death within 3 months, and the optimal cut‐off level was 274.59 ng/mL. The serum GP73 levels significantly decreased and remained stable at approximately 6 months for survivors. Conclusion Serum GP73 may serve as a valuable biomarker for the diagnosis and prognosis prediction of HBV‐ACLF patients.

As a noninvasive marker, serum alanine aminotransferase (ALT) has limitations, because a large proportion of patients chronically infected with hepatitis B virus (HBV) suffer from severe hepatic necroinflammation, but have normal or mildly elevated ALT. In the present study, we aimed to investigate the potential value of serum Golgi protein 73 (GP73) in predicting significant hepatic necroinflamation among chronic HBV infected patients. A cohort of 497 chronic HBV infected patients was retrospectively recruited. Liver biopsy was performed in all patients and serum GP73 levels were measured by enzyme-linked immunosorbent assay. Serum GP73 increased in parallel with the increase in hepatic necroinflammatory activity grade (r = 0.682) and the stage of liver fibrosis (r = 0.539). The positive correlation of serum GP73 with the degree of hepatic necroinflammatory activity was statistically significant, while serum GP73 with the stage of liver fibrosis was weaker than that with hepatic necroinflammation. Furthermore, serum GP73 levels were significantly greater in patients with normal or mildly elevated ALT and significant hepatic necroinflammation (≥G2) than in patients with minimal to mild hepatic necroinflammation. The sensitivity and specificity of GP73 for the diagnosis of G2 hepatic necroinflammation was 42.35% and 95.0%, respectively, at a cut-off value of 88.38 ng/mL. When the cut-off value was set at 124.76 ng/mL, the sensitivity and specificity of GP73 for the diagnosis of G3 hepatic necroinflammation was 55.56% and 97.29%, respectively. These findings indicate that GP73 holds promise as an important candidate for diagnosing significant hepatic necroinflammation.

本文重点研究了国家质量基础(简称NQI)的概念、作用和意义、国内的发展现状,以及NQI在设备监理行业中的运用,简要介绍了四川科特在NQI工作中一些做法,为设备监理行业未来在NQI的建设工作中提供依据.

The Department business learning credit system management was put into practice in the authors'hospital from the end of 2011.Quantitative assessment was used to realize the whole process of supervision and multi-dimensional evaluation.As a result, the department learning atmosphere became stronger, the develop-ment of personal growth and department were promoted, led to the comprehensive development of the hospital's medical, teaching, research, and the connotation construction.

BACKGROUND: The World Health Organization (WHO) has estimated that the number of cancer patients will increase by about 70% during the next 25 years world-wide. To deal with this problem, WHO has suggested a focus on prevention of tumor incidence and health screening for early detection of people with tumors.OBJECTIVE: To investigate the use of thymidine kinase 1 (TK1), CEA and AFP in serum to discover people with malignant tumors through health cancer screening.METHODS: Of a cohort in 486,085 people of a routine health screening at the Health Centre, Fujun 180 Hospital, Quanzhou city, China, 56,286 people were investigated according to the presence of cancer during 2009-2014. The concentration of CEA and AFP were determined by an electrochemiluminescence immunoassay from Roche Diagnostics e601GmbH and STK1 by a commercial kit based on an enhanced chemiluminescent dot blot assay.RESULTS: The cancer incident rate increased from 0.048/100,000 to 0.220/100,000. The most common types of tumors were those of the liver, cervix and lung. STK1 correlated to tumor growth rate, was more sensitive than CEA and AFP for discovering people with malignant tumors and more sensitive among people who had diagnosis of malignant tumor. STK1 was also a prognostic biomarker for death at 10-40 months follow-up, while CEA and AFP were not. A combination of these markers increased the sensitivity by about 30%.CONCLUSION: STK1 is a reliable biomarker for discovering people with malignant tumors in cancer screening.

Objective: To explore the application effects of opening the academic exchange forum on the local area network for hospital professional study. Method: Since January, 2013, the hospital has set an academic exchange forum on the local area network. Periodically upload some basic fundamental knowledge lessons, academic reports, the latest specialty analysis, the typical cases discussion and other posted contents so as to offer hospital staff a chance to download the materials and join the discussion, while the administrators periodically organize discussions and answer doubts. Collect and compare the average scores information of each staff who attended the three-fundamental knowledge examination organized in each season of 2013 and 2014. Results: Compared the average scores of the hospital staff in 2013 with those of the 2014, the latter have been improved greatly and their differences have statistical meanings(P<0.05). Conclusion: The establishment of the academic exchange forum on the local area network not only has improved the staff enthusiasms of studying the professional specialties, but also has enhanced the effectiveness of the academic professional specialties teaching. An uplifting, interesting, competing studying atmosphere has been built in the hospital, moreover the cooperation and mutual assistance of each teaching and research office and each department have been strengthened and promoted.

Objective To investigate the efficacy of intrahepatic transplantation of autologous bone marrow mesenchymal stem cells (BMSCs) in the treatment of patients with decompensated alcohol-induced liver cirrhosis (ALC). Methods Twenty-eight patients with decompensated alcohol-induced liver cirrhosis were recruited for intrahepatic transplantation of BMSCs from 2007 to 2012,and other 28 patients with ALC were selected by propensity scores matching for age,gender and complications of liver cirrhosis. Results The serum alanine aminotransferase levels declined to nearly normal levels,total bilirubin decreased,albumin (ALB) increased, prothrombin times shorten,and Child-Pugh scores (CPS) were improved gradually;The model of end stage liver disease scores were significant decreased to (20.6±5.2) at forth week,and significantly lower than in the control group(P=0.009),however,there was no remarkable differences after 72 weeks (P=0.536) between the two groups;The survival rates at 144 weeks in the two groups were not significantly different (32.1% vs. 25.6%,P=0.125);Further analysis showed that the survival rates in patients with CPS class B in the two groups were significantly different (66.7% vs. 36.4%,P=0.046),while there was no significant difference in patients with CPS class C (12.5% vs. 17.6%,P=0.788). Conclusions The short-term efficacy of BMSCs transplantation in the treatment of patients with decompensated ALC is favorable,but the long-term efficacy needs further investigation.

科技创新是医院持续发展的不竭动力，是医院转型发展的必由之路，军队中小医院目前正处于发展的机遇期和转型的关键期，机遇和挑战并存。本文从军队中小医院自身实际出发，阐述了科技创新在建设研究型医院中地位作用，提出了加强医院科技创新的着力点以及制度化、常态化推进办法，旨在探讨如何实现新形势下军队中小医院向研究型医院转型转变的发展。

Dendritic cells (DCs) are multifunctional cells that initiate adaptive immune responses. Patients with chronic hepatitis B virus (HBV) infection have reduced numbers of DCs which may be functionally impaired, a defect that may contribute to viral persistence. Autologous DC-based immunotherapy is considered to be a treatment option for chronic HBV infection (CHB). We evaluated the therapeutic efficacy of HBV-pulsed DCs in combination with the antiviral drug entecavir in patients with CHB. Eighty patients were divided into four groups: HBV-pulsed DCs only, HBV-pulsed DCs plus entecavir, entecavir only, and an untreated control group. Patients on combination therapy exhibited greater antiviral responses than patients on either monotherapy. The combination of HBV-pulsed DCs and entecavir resulted in the largest reduction in serum viral DNA levels and the highest percentage of virologic response. In addition, combination therapy resulted in viral e antigen (HBeAg) loss and seroconversion. These results suggest that the combination of HBV-pulsed autologous DCs and entecavir could be therapeutically advantageous for patients with CHB.

Objective To discuss the influence of compensation scheme of medicare outpatient with spe-cial diseases on patients overall satisfaction .Methods We took samples from the random questionnaires distribu-ted to patients who came to the department of neurology of our clinic From October 2014 to December 2014 , and compared the overall satisfaction of patients who bought the compensation scheme for special diseases and that of those who did not buy .Resutls A total of 487 questionnaires were taken back , among which 298 were written by males, 189 by females, aged 18 to 92 years ( with an average age of 53.4 ±27.8).The number of people who had healthcare was 174 whose overall satisfaction was 85.32 ±10.21(%), while the overall satisfaction of the 313 people in the control group was 73 .18 ±15 .84 (%) .The overall satisfaction of the former group was obviously higher than that of the control group, and the difference was statistically significant (P<0.05).Conclusion Compensation scheme of medicare outpatients with special diseases help strengthen patients' compliance, improvedtreatments, reduced economic burden, significantly improved patients overall satisfaction towards hospital and doc -tors, which greatly improved the doctor -patient relationship.

Objective To discuss the application effect of WeChat group interaction teaching mode in the clinical department vocational study.Method We selected the neurology as the pilot department.We implemen-ted the centralized classroom teaching on every Tuesday night and WeChat group interactive questions and answers, discussion on every Wednesday night taken turns by members of the department from January 2013 to December 2013.We compared it with professional theory examination scores of each month under WeChat group of interactive teaching in 2012 and the examination results of each quarter’s hospital three kinds of basic theories (neurology pro-fessional knowledge).Results Each month specialized theoretical examination average scores of 2013 improved more obviously than 2012, and the difference was statistically significant ( P <0.05); every quarter’s average point of hospital three kinds of basic theories examination has increased significantly, and the difference was statisti-cally significant (P <0.05).Conclusion Our hospital implemented the model of instruction of WeChat group in-teraction on the basis of cyber interactive, which greatly improved the learning enthusiasm of medical personnel, significantly promoted the doctors’understanding of knowledge and learning and greatly improved the effectiveness of the business learning.It is worth popularizing widely.

HSE培训是建立HSE管理体系中很重要的一个步骤，HSE体系的执行力也离不开员工的参与，通过培训增强员工的HSE责任意识，保障员工的健康安全，同时也是提高作业现场员工按章操作主观能动性的助推剂，是否经过培训和培训效果的好坏也是决定HSE管理体系是否长效运行的重要因素之一。

本文介绍一种具有打捞带独立多气囊淡水袋式救生衣.该救生衣克服了目前国内外使用的泡沫填充式和单气囊气胀式救生衣穿着不舒适、水中阻力大、不方便打捞,海上易翻滚、易破损漏气、不能贮存淡水等缺点,具有体积小、重量轻、穿着舒适,多个独立气囊,打捞方便,落水后能保证头面朝天,延长漂浮时间等优点,从而为部队海上训练和作战、抗洪抢险、水上作业等提供有力保障.

<正>为解决官兵水上训练或作战时,水上救生衣因无打捞带而不方便打捞,以及受伤官兵落水后无法自动改变体位以保持头面部朝上等问题,我们设计了能够自动充气膨出的救生衣打捞带。现将该救生衣打捞带研制体会报告如下。

The body of a valve is one of the most important parts of the valve,and the design and improvement of valve is important for new products and old products.To design a valve body with uniform strength and improve the valve structure for decreasing accumulation of stress is a much discussed topic in the machinery industry.In this paper,we first build the three dimensional model for the major assembly of a flat valve,such as its valve body,valve plate and valve cup,and carry out the assembly process in a virtual environment by SolidWorks.Then we mix them seamlessly by SolidWorks and Algor.Finally we insert the major parts into Algor and carry out finite element analysis and find out the stress accumulation points according to finite element analysis results.We also present measures for improvement according to the result.

在油气开采过程中,随着烃类流体的不断采出,油藏内部的孔隙压力随之下降,在外部没有任何补给压力的情况下,储层压力发生衰竭是必然的。油藏的这种变化不仅对孔洞中流体的流动状态有影响,储层岩石的微观和宏观结构也必然会产生一定的变化,宏观表现如压实、地面沉降、套管变形,以及地层滑移、裂缝的闭合和再生等;微观表现如孔隙度和渗透率的变化、孔喉中毛管压力以及流体渗流特性的变化等。压力衰竭引发的一系列问题给钻井、完井以及开发方案的制定和调整提出了新的挑战。

本文阐述了军队医院网络医疗服务新模式的构建和应用,即运用网络信息化技术,并结合最新的为兵服务理念、良好的医院管理理念,在部队伤病员就医流程中提供方便、快捷的信息化就医服务,实现军队医院与基层卫生机构的信息化联合管控,增强远程教学帮带作用,从而提高军人就医效率,增强部队防治能力,确保部队战斗力。

2004年以来,我院充分利用"军区部队卫勤信息化中心"的人才、技术和设备优势,对"军队医院面向体系部队网络医疗服务"的课题进行了深入研究,创建了我院独特的军人就诊网络化服务新模式.现将我们的主要做法报告如下.

目的探讨甘草酸二铵联合促肝细胞生长素治疗慢性乙型肝炎(CHB)的临床疗效。方法172例CHB患者随机分为A、B两组。A组采用甘草酸二铵联合促肝细胞生长素治疗,B组甘草酸单铵联合丹参治疗,疗程2个月。结果治疗后A组肝功能、TNF-α及IL-6、IL-8与对照组对比,差异有显著性(P<0.05),A组肝组织TGF-β表达显著下降,与对照组对比差异有显著性(x2=6.538,P<0.05)。结论甘草酸二铵联合促肝细胞生长素有明显改善肝功能、抑制炎症反应和抗肝纤维化等作用。

声发射作为一种成熟的无损检测技术在航天航空、采矿、土木工程、电力、交通运输等方面都有广泛的应用,声发射技术作为一种非常规的无损检测手段,在石油工程领域的应用也有越来越多,并为石油钻井、完井、油气开采等各项施工作用提供理论依据。本文主要建立在声发射与材料的力学性质的关系上,参考国内外文献资料,综合声发射在油气田开发中的应用,为以后的声发射技术研究提供参考。