The pressure on people’s lives and work is increasing with the continuous development of modern society, and generalized anxiety disorder (GAD) has become a relatively common psychological problem. Nevertheless, the pathological mechanisms underlying anxiety disorders remain poorly understood, and specific biomarkers available for identification and clinical diagnosis are still lacking. Serum samples were collected from 20 patients with GAD and 20 healthy controls. Untargeted metabolomics based on liquid chromatography-mass spectrometry (LC-MS) was used for detection and analysis to screen differential metabolites and perform correlation analyses. Compared with those in healthy controls, a total of 1271 differential metabolites were identified in patients with GAD, including 986 upregulated metabolites and 285 downregulated metabolites. Among all the identifiable metabolite classes, the top six categories were lipids and lipid-like molecules (32.53

To identify the differentially expressed peptides that influenced the generalized anxiety disorder (GAD) by adopting peptidomics and to further provide new targets for the diagnosis and treatment of clinical GAD. 20 patients with GAD as the experimental group and 20 healthy volunteers as the control group were included in the experiment. Differentially expressed peptides were compared and analyzed after collecting serum samples from the two groups and performing peptidomics testing. In addition, GO analysis, COG analysis, and enrichment pathway analysis were utilized to explore the biological processes and function distribution of the differentially expressed peptides. A total of 1149 significantly differentially expressed peptides were identified in the sera of patients with GAD compared to healthy controls (p< 0.05 or P-value-chitest< 0.05), the up-regulated polypeptides are predominant among them. Bioinformatics analysis revealed that the differentially expressed peptide precursor proteins are primarily involved in biological processes such as cellular cytoskeleton organization, intracellular signal transduction, and vesicular trafficking, and are primarily localized in subcellular structures such as the cytoplasm and vesicles, suggesting a possible role in the pathogenesis of GAD by influencing cellular cytoskeletal dynamics, synaptic plasticity, and intercellular signal transduction. Further analysis revealed that the corresponding proteins of these significantly differentially expressed peptides (such as SLAM family member 5, Talin-1, Clusterin, and IGHG1) are involved in immunoregulation, integrin activation, neuroprotection, and antibody-mediated immune responses, indicating that immune-inflammatory imbalance, abnormal synaptic structure, and function may be important molecular mechanisms in the pathogenesis of GAD. GAD is closely associated with immune-inflammatory imbalance and synaptic abnormalities, with significant differences in key proteins such as SLAM family member 5, Talin-1, Clusterin, and IGHG1. This study preliminarily explores differentially expressed peptides in GAD, providing new insights for clinical diagnosis and the screening of therapeutic targets.

臀上皮神经卡压综合征是临床常见的疾病之一，具有病程长、临床表现不典型、易误诊等特点。由于现代人们长期的姿势不良，因此造成近年来的发病率呈逐渐增长的趋势，严重影响人们的生活。临床中治疗本病多数采用非手术治疗，而推拿作为“无创，无痛”疗效确切的绿色疗法，非常值得推广。近年来，医学界逐渐重视本病，并且在推拿手法治疗中取得一定的研究进展。本文基于上述背景，对推拿治疗臀上皮神经卡压综合征的研究进展进行探究。通过检索关于臀上皮神经卡压综合征的文献，发现推拿及推拿结合其它中医疗法治疗该病的效果良好。通过收集及整理不同治疗该病的推拿方法，为以后优化该病的临床治疗方案提供借鉴。

目的:观察基于筋骨平衡理论采用中药溻渍治疗慢性非特异性腰背痛(chronic nonspecific lowback pain,CNLBP)的临床疗效.方法:选择河南中医药大学第三附属医院推拿科门诊招募的 CNLBP 患者 100 例,采用数字随机分组法分为两组,每组 50 例.对照组给予塞来昔布胶囊,200 mg/次,1 次/d,餐后半小时口服.若疼痛较重,可改为 200 mg/次、2 次/d,病情好转后再恢复为 200 mg/次、1 次/d.连续服用 14 d.治疗组在对照组治疗基础上加用局部中药溻渍治疗,同时使用 TDP 灯照射,20～30 min/次,2 次/d.连续治疗 14 d.结果:治疗后,治疗组疼痛视觉模拟评分法评分和 Oswestry功能障碍指数下降程度优于对照组,前屈、后伸、健侧旋转、患侧旋转的活动度较对照组改善更明显,塞来昔布胶囊的药物用量和人均使用时间均少于对照组,差异有统计学意义(P＜0.01).结论:基于筋骨平衡理论采用中药溻渍治疗 CNLBP 能够缓解疼痛,改善腰部功能和腰椎活动度,减轻西药用量,提高患者生活质量,值得临床推广运用.

正脊,作为推拿重要的一部分,除了对疼痛类疾病有良好的疗效外,临床试验中亦发现其对脊源性内科疾病的治疗也有良好的效果.对近年正脊治疗脊源性内科疾病的文献进行归纳总结发现,从中医的角度分析,正脊通过调节阳气、调节督脉、膀胱经对脏腑起到良好调节作用,从而实现治疗脊源性内科疾病的目的;从现代医学的角度分析,正脊通过恢复力学平衡、缓解神经受压迫状态、调节免疫、调节内分泌的途径实现脊源性内科疾病的治疗.

功能性腹胀主要发病机制为中焦气机不利,运化升降失常,属本虚标实之证,与肝、脾、胃三脏关系密切.郭现辉教授在辨证思想体系指导下,基于该病本虚标实之证,采用"益气通经"推拿法治疗功能性腹胀.根据经脉所过,主治所及的思想,该法主要作用于腹部任脉经穴,以运行周天,调和阴阳.操作手法上以指针法重点按揉气海穴,发挥气海主治一切气机之病的作用,结合旋推法施术上、中、下脘穴,最后与摩腹法相配合达到补益元气、通经调腑、畅达气机、消胀除满的作用.

颈源性高血压是继发于颈椎病的血压升高,临床治疗中若只关注高血压的表象而忽视颈椎病的病理学基础,则不能取得良好、稳固的疗效.中医药治疗颈源性高血压重视标本兼顾,多以舒筋活络、潜阳平肝、滋补肝肾等药物同用;针灸常选取颈部腧穴以疏通经络,并常与足三里、三阴交等特定穴位配合;推拿主要采用理筋松解、正骨等恢复颈椎的生物力学平衡.中医药治疗遵循治病求本、标本兼治的原则,在提高疗效的同时,还可保持疗效的稳定.但目前针对颈源性高血压尚未形成统一的诊断标准,未来应加强对其诊断方法的探究,做到早诊断、早治疗,促进患者康复.

颈源性失眠是临床常见的一种病症,失眠是主要表现,但根本原因在于各种原因导致的颈椎病.临床医师在治疗失眠时常从内科方面考虑,很容易忽视颈椎病的原因.临床治疗中,中药治疗颈源性失眠多采用舒经通络、安神定志的方药,针灸多采用头颈部腧穴以及内关等特定穴治疗,推拿则主要采用正骨、点穴等手法进行治疗,取穴多与针灸相近.此外,针灸推拿常配合腹部及背部腧穴治疗,调整脏腑功能,由外而治,由内而愈,最终使疾病内外同治,标本兼顾,既可提高疗效,又可提高其治疗的稳定性.

推拿学属于中医学的范畴,是以中医基础理论为指导,阐述和研究运用手法和功法防治疾病的方法、规律和原理的一门医学学科,遵循中医学哲学思想体系,具有统一的哲学思想体系和系统.本文通过对推拿的历史源流、相关理论著作与当今发展状况的介绍,并不断促进思政教育与推拿专业教育相融合,使人们认识到推拿事业具有广阔发展前景.在科学研究的基础上实事求是,坚持一切从实际出发,使推拿学更具有说服力、权威性.

对于推拿医学生后期临床教学而言,如何理论结合实践,动手不忘动脑,动脑促进动手,才会真正进步.因此引进新的教学模式:如功法每日练、由学生当带教讲解的小课堂、临床技能pk的科室大练兵,可为枯燥的专业知识学习增加活力,也会增加学生学习的主观能动性,变学生为教学的主体,从而调动学生学习的积极性,深化重点内容的记忆和掌握.达到学生教、学相长效果,培养合格的推拿学科临床医生.

颈源性头痛是一种常见病、多发病.近年来,随着工作方式的改变,该病的发病率日益增高.通过查阅资料发现中医学治疗该病疗效显著,尤其是推拿治疗.通过收集推拿治疗该病的不同方案,发现推拿治疗颈源性头痛疗效肯定,对病情均有不同程度的改善,为以后优化该病的临床治疗方案提供参考.

推拿学作为中医学的一部分,旨在培养拥有完备的诊断治疗知识体系,并且道德品质高尚,能够全心全意为患者服务的医生.在当今社会的医疗背景下,为了培养出优秀的医生,响应课程思政改革的号召,就要把课程思政应用到推拿学教学工作中去.文章从三个角度探讨课程思政在推拿学中的应用:把课程思政应用到推拿学教育中的必要性、把课程思政应用到推拿学的具体措施、关键问题的解决.希望能制订一套完整的推拿学教育的具体方案,提高学生的思想道德素质,培养拥有完备知识体系并且道德品质高尚的医学人才.

OBJECTIVE:Study the effect of electroacupuncture on permenopausal depressive disorder (PDD) model through the peri-menopausal depression model mice. METHODS:KM female mice were selected. Except for the blank group (BG), the other groups of mice were removed by castration method. The mice of PDD was prepared by combining chronic unpredictable stimulation. Mice in the model group (MG) were not treated and fed normally. The western medicine group (WG) was given the corresponding drug for treatment. The electroacupuncture group (EAG) was given the electroacupuncture for treatment, and consecutive for 28 days. The levels of T, E2, FSH and LH in serum of mice were measured, and the brain tissue of 5-HT, DA and NE level were measured. Through the HE staining observed the morphological changes of mice hypothalamus. RESULTS:Compared with MG, EAG could increase the number of spontaneous activities of PDD model mice, the level of T, E2 in serum and the level of 5-HT, DA, NE in brain tissue was improved, and the level of FSH, LH in serum was reduced, and the hypothalamic lesions was improved. CONCLUSION:Electroacupuncture could improve the activity and memory of PDD mice, adjust the disorder of sex hormone, and increased the levels of monoamine transmitters (5-HT, NE, DA), and it could effectively improve the behavior and related biochemical indexes of PDD, and thus play an important therapeutic role.

微课作为一种新型的教育信息资源形式,以“主题突出、精简、交互性好、应用面广”等特点被广泛认可,已成为我国当前教育信息化资源建设的研究热点和重点.推拿手法学是我国传统医学的瑰宝,是一门既古老而又新型的学科,是推拿基础和推拿临床的桥梁,推拿手法实训对培养合格推拿医师至关重要.笔者通过开展微课教学使学生更容易掌握相关知识,能积极调动学生的学习积极性和对新知识的探索.在推拿手法教学中运用微课教学有明显的效果.

目的 观察平乐正骨理论治疗膝关节寒痹的临床疗效.方法 随机选取60例确诊的膝关节寒痹的患者,给予平乐正骨历节风方、特异性点穴手法、平乐正骨二乌散、温针灸及功能锻炼疗法,观察其疗效,并对治疗前后进行评分比较.结果 总有效率为98.34％,其中临床控制16例(26.67％),显效21例(35.00％),有效22例(36.67％),无效1例(1.66％);膝关节功能评分治疗前后的比较,P ＜0.05,差异有统计学意义.结论 运用平乐正骨理论治疗膝关节寒痹的临床疗效显著,能够在广大社区医院推广应用.

儿科历来素有哑科之称,自古有望而知之谓之神之说,望诊在小儿疾病诊断、治疗及判断预后方面具有非常重要的意义.故通过总结历代医家对山根的认识,尤其是山根脉络的颜色、位置、色泽、脉络的分散或聚集的意义,结合多年的临床实践并进行总结,系统阐述山根脉络的变化在小儿疾病诊断、治疗中具有重要的指导意义,特别是山根青色脉络的位置、色泽及脉络的聚集与否在指导小儿推拿治疗脾胃病及对疾病的判断、治疗、预后方面的应用具有重要指导意义.

神经根型颈椎病是颈椎退行性改变导致颈部脊神经根受压或刺激的疾病,属于脊柱筋骨疾病.笔者基于平乐郭氏正骨提出的筋骨互用平衡理论以及多年临床经验,从“筋、骨”方面指导该病的诊治.在治疗该病时运用手法、功能锻炼及中药外敷等综合疗法,进行“理筋、养骨”.在中医辨证基础上配合中药以调理气血,补益肝肾,恢复机体的筋骨平衡.文章即从“筋、骨”平衡方面来探讨神经根型颈椎病的临床诊疗思路,旨在为临床治疗该病提供可靠的临床依据和资料.

目的:观察透穴埋线治疗单纯性肥胖症的临床疗效.方法:选择河南中医药大学第三附属医院针灸门诊收治的单纯性肥胖症患者1206例,给予透穴埋线治疗(中脘、梁门、天枢、大横、阴交、章门、京门、丰隆、局部阿是穴加减)治疗1次为1个疗程,30 d后判断疗效.结果:男性及女性使用透穴埋线减肥的疗效对比无统计学意义(P>0.05);透穴埋线治疗肥胖症的疗效与其肥胖程度基本成正比(P<0.01);青少年患者的疗效优于中、老年患者;春季和夏季治疗的患者疗效优于秋季患者.结论:透穴埋线法减肥的疗效和性别无明显关联,和肥胖度基本成正比;青少年比中、老年患者疗效显著;春、夏季比秋冬季疗效显著.

经脉、络脉、血脉有何区别，临床在诊脉的过程中，诊的是经脉还是血脉，这些问题一直有所争议，本文对三者之间的区别以及其与脉诊的关系浅谈自己的拙见。

Objective To observe the clinical efficacy of acupoint thread -embedding combined with pidotimod therapy on children's recurrent respiratory tract infection at non-acute phase. Methods Seventy cases of children with recurrent respiratory tract infection were randomly divided into the treatment group and control group. Control group applied pidotimod oral therapy once a day in the morning on an empty stomach. Patients in the treatment group were treated with same therapy of the control group as well as acu-point thread-embedding treatment at the main acuoints of Dazhui,Fengmen,Feishu,Gaohuangshu,Pishu, Shenshu,Guanyuan,Zusanli. After three months'treatment,the two groups were compared to observe its clini-cal efficacy and Immunological indicators. Results The effective rate of the treatment group was 97. 14%, and the effective rate of the control group 82. 86%. The comparative difference between the two groups had statistical significance(P﹤0. 05);the immunological indexes all increased at different degrees after the treat-ment in the two groups(P﹤0. 05 or 0. 01),and the IgG,IgM,IgA for the treatment group increased signifi-cantly compared with the control group after treatment(P﹤0. 05 or 0. 01). This indicated that the treatment group was better than the control group. Conclusion The clinical efficacy of acupoint thread-embedding therapy combined with pidotimod therapy in non-acute stage of children with recurrent respiratory tract in-fection is more effective than pidotimod therapy.